CN102951957A - Microbial viable bacterium fruit tree water flush fertilizer, preparation technology and application method thereof - Google Patents
Microbial viable bacterium fruit tree water flush fertilizer, preparation technology and application method thereof Download PDFInfo
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Abstract
The invention relates to a microbial viable bacterium fruit tree water flush fertilizer, a preparation technology and an application method thereof. The improvement is that the microbial viable bacterium fruit tree water flush fertilizer comprises the following components in parts by weight: 30-50 parts of brown sugar, 3-10 parts of yeast extract, 5-10 parts of microbial agent, 0.2-0.4 part of borax, 0.2-0.4 part of magnesium sulfate, 0.3-0.6 part of ferrous sulfate, 0.003-0.006 part of potassium dihydrogen phosphate and 0.2-0.5 part of manganese sulfate. The fruit tree water flush fertilizer improves the blooming rate, fruit setting rate and yield of the fruit tree so that the blooming rate of the fruit tree is improved by 20%, the fruit setting rate is improved by 30% and the yield per plant is improved by 21%.
Description
Technical field
The present invention relates to fertilizer, specifically, relate to fruit tree punching fertilising, preparation and method of application thereof.
Background technology
China is No.1 populous nation in the world today, large agricultural country especially, and the agricultural of China has been supported the population in the world 22% in the arable land in the whole world 7%, gasp in admirationd the large miracle for human history.Yet the ground contradiction of having a large population in a small area is more and more fiery.Agricultural be to plough at all, the arable land be soil at all, soil be fertility at all.But because of long-term application chemical fertilizer and its amount of application of cumulative year after year, its negative effect has allowed common people exclaim, it has not only caused, and soil compaction, soil property change, the regenerative power of soil is very low, fruit quality descends, and returns the ecotope that the mankind depend on for existence and has caused destruction in various degree.
Application number: 200610068499.5, denomination of invention be " a kind of liquid composite microorganism fertilizer and preparation method thereof " disclose " a kind of preparation method of liquid composite microorganism fertilizer; step is as follows: (1) is with cereuisiae fermentum; plant lactobacillus and Lactobacterium acidophilum are bacterial strain, inoculates respectively Erlenmeyer flask and prepares second-class liquid isolate; (2) by weight with potato 1-10 part, brown sugar 1-10 part, dipotassium hydrogen phosphate 0.01-0.2 part, sodium-chlor 0.1-2 part is mixed, and adds clean water 69.6-96.8 part dissolving, and heat treating method is sterilized, as substratum; (3) in above-mentioned substratum, inoculate first the secondary sterilization of liquids of cereuisiae fermentum, cultivated 16-24 hour for 28-32 ℃, and then 1: 1 mixed solution of the second-class liquid isolate of inoculation plant lactobacillus and Lactobacterium acidophilum, continuing to cultivate 24-30 hour at 34-37 ℃, the pH value is down to 3.5-4.5 and is got final product; The inoculation total amount is the 0.5-4 weight part; (4) product of step (3) is aseptic subpackaged, make liquid composite microorganism fertilizer." this patent application proposes for the utilization ratio of disease-resistant performance and fertilizer; and test for tomato; its stimulation ratio is 23%; test for cucumber; its stimulation ratio is 25.2%; but owing to only having dipotassium hydrogen phosphate and sodium-chlor so that the nutritive ingredient of plant is obviously not enough, the Comprehensive that needs for plant-growth does not replenish its needed nutrition, and liquid form, the difficult heavy dose packing that realizes, and its cost that needs of packed liq fertilizer is handed over high, therefore, is unfavorable for industrial large-scale production.
Application number: " 200710093109.4, denomination of invention is that " fowl dung fermentation fertilizer and preparation method thereof " discloses that " a kind of fowl dung fermentation fertilizer is characterized in that, described fertilizer comprises that following raw material makes; Drying rear weight is the fresh chicken manure of 600-700 kilogram, 246.8-371.2 kilogram auxiliary material, 1.6-2.4 kilogram zymophyte I and 2.3-3.6 kilogram fermented bacterium II; Wherein, auxiliary material comprises: 200-300 kilogram chaff powder, 20-30 kg corn face, 15-20 kilogram wheat flour, 3-8 kilogram brown sugar, 8-12 kilogram lime powder ... "
Not processed or become thoroughly decomposed and be applied directly on the crop owing to chicken manure, can there be very large harm and hidden danger, be mainly manifested in:
1. transmissible disease insect pest.Contain germ and the insects such as colibacillus, nematode in the ight soil, directly use can cause propagation, the crop morbidity of disease and pest, and the HUMAN HEALTH of food agricultural produce is also exerted an influence; When the humus organic material material does not ferment, easily grow germ and insect pest in soil, also cause the generation of plant pest;
2. seedling is burnt in fermentation.After the organic materialss such as azymous living excrement are executed in the ground, when fermentation condition possesses, at the movable bottom fermentation of microorganism, near and crop plant is hour apart from root when the fermentation position, the heat that fermentation produces can affect plant growth, burns crop root, causes plant dead when serious.
3. poison gas harm.In decomposition course, produce the obnoxious flavoures such as methane, ammonia, make soil and make deposits yields acid evil and the root system damage.
4. soil anoxic.The organic oxygen that consumes in decomposition course in the soil makes the temporary anoxic condition that is in of soil, and plant growth is suppressed.
5. fertilizer efficiency is slow.Nutrient mostly is not organic or slow attitude in the organic fertilizer of fermentation maturity, can not directly be absorbed by crop, only has decomposition and inversion to become the utilization absorbed by crops of quick-acting attitudes ability, so when the fertilizer that do not ferment is directly used fertilizer efficiency is slowed down.
6. the high unprocessed direct use of cost, the organic materials volume is large, and effective constituent is low, transportation inconvenience, use cost improves.
Based on defects, chicken manure must through becoming thoroughly decomposed fully, therefore need strict red-tape operati, and above-mentioned patent not provide effective explanation for fully becoming thoroughly decomposed of chicken manure before using.
In addition, application number: " 200710016131.9, denomination of invention is that " bio-feritlizer " discloses " a kind of bio-feritlizer, it is characterized in that: described bio-feritlizer comprises Ah's rhzomorph mixture, ammonium sulfate, monoammonium phosphate, vitriolate of tartar, borax, zinc sulfate, ferrous sulfate, calcium powder, root-growing agent, brown sugar, chicken manure, zanthoxylum powder, peanut cake or Semen Ricini cake." this patent application patent discloses the combination of brown sugar and ferrous sulfate; there are simultaneously Ah's rhzomorph mixture and chicken manure; it proposes for disease; and cost can reduce 20-30%; but how many not concrete its stimulation ratio can realize; and fruit-setting rate is all less than explanation, in addition, owing to there being harmful unwanted bacteria in the chicken manure, Ah's rhzomorph mixture is with when chicken manure mixes, need to destroy the unwanted bacteria in the part chicken manure, and then carry out the fermentation of other components, finally cause the disease problem of plant easily to produce the defective of undertreatment.
Summary of the invention
The object of the invention is in view of the foregoing defects the prior art has, a kind of microorganism live bacteria fruit tree punching fertilising is provided, this fruit tree punching fertilising can obviously improve blooming, bear fruit and output of fruit tree, the fruit tree flowering rate is improved reach 20%, fruit-setting rate improve reach 30%, single plant yield improves and reaches 21%.
A kind of microorganism live bacteria fruit tree punching fertilising provided by the invention, its improvements are to comprise the by weight following component of component meter: 25-50 part brown sugar, 3-10 part yeast extract paste, 5-10 part microbiobacterial agent, 0.2-0.4 part borax, 0.2-0.4 part sal epsom, 0.3-0.6 part ferrous sulfate, 0.003-0.006 part potassium primary phosphate and 0.2-0.5 part manganous sulfate.
The provided by the invention first preferred microorganism live bacteria fruit tree punching fertilising comprises the by weight following component of component meter: 25-45 part brown sugar, 5-9 part yeast extract paste, 6-9 part microbiobacterial agent, 0.25-0.35 part borax, 0.25-0.38 part sal epsom, 0.32-0.55 part ferrous sulfate, 0.0035-0.0056 part potassium primary phosphate and 0.22-0.47 part manganous sulfate.
The provided by the invention second preferred microorganism live bacteria fruit tree punching fertilising comprises the by weight following component of component meter: 30-40 part brown sugar, 7-8 part yeast extract paste, 7-8 part microbiobacterial agent, 0.28-0.3 part borax, 0.3-0.35 part sal epsom, 0.4-0.5 part ferrous sulfate, 0.004-0.005 part potassium primary phosphate and 0.3-0.4 part manganous sulfate.
The provided by the invention the 3rd preferred microorganism live bacteria fruit tree punching fertilising, described microbiobacterial agent is bacterium, yeast, fungi and actinomycetes.
The provided by the invention the 4th preferred microorganism live bacteria fruit tree punching fertilising, described bacterium are one or more the mixed bacterium that is selected from subtilis, Bacillus licheniformis, bacillus cereus, the pseudomonas; Described yeast is Pichia yeast and/or intends the ball yeast; Described fungi is trichoderma harziarum and/or viride; Described actinomycetes are Streptomycin sulphate.
The Bacillus licheniformis (Bacilluslicheniformis) of the provided by the invention the 5th preferred microorganism live bacteria fruit tree punching fertilising, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center (being called for short CGMCC), address: No. 3, No. 1 institute in Beichen Lu, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101, its deposit number is CGMCC No.4920; Preservation date is on 06 01st, 2011, and the preservation name is called POLO-BLN1103.
The preparation technology of the fruit tree punching fertilising of microorganism live bacteria provided by the invention may further comprise the steps:
1), preparation composite flora: with above-mentioned bacterium, yeast, fungi and actinomycetes by weight, and press (1-2): (1-5): (0.5-7): part by weight (1-2) is made composite flora;
2), spawn culture: culture dish is placed in the 27.1-28.5 ℃ of incubator to cultivate chose bacterium in 3-7 days, bacterium is chosen in after cultivate the 3rd day in batches continuously, to reduce the interference of miscellaneous bacteria, select single bacterium colony according to colonial morphology when choosing bacterium colony, purifying is transferred to slant medium;
3), zymotechnique
Adopt distilled water by 200: the volume ratio of 0.01-0.1 dissolves into the aqueous solution with above-mentioned brown sugar, yeast extract paste, microbiobacterial agent, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight, when water temperature is 18-22 ℃ above-mentioned composite bacteria and yeast extract paste is by weight put into the aqueous solution; Stir every day and once make it even, the secondary fermentation surface grew white hair in 2-3 days; Through stirring once 2-3 days every days, foam occurs and discharge simultaneously pure fragrance again, fermentation stops to obtain fermented liquid, and this moment, sugar decomposition changed into the glucokinase activity higher alcohols; Add above-mentioned trace element by weight: first trace element is dissolved with warm water, be added in the fermented liquid and mix.
The method of application of the fruit tree punching fertilising of microorganism live bacteria provided by the invention, its improvements are:
2), spawn culture: culture dish is placed in 28 ℃ of incubators to cultivate can chooses bacterium in 3 days, chose continuously bacterium in the 3rd day after cultivate in batches, to reduce the interference of miscellaneous bacteria, select single bacterium colony according to colonial morphology when choosing bacterium colony, purifying is transferred to slant medium;
3), zymotechnique:
Adopt distilled water by 200: the volume ratio of 0.01-0.1 dissolves into the aqueous solution with above-mentioned brown sugar, yeast extract paste, microbiobacterial agent, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight, when water temperature is 20 ℃ above-mentioned composite bacteria and yeast extract paste is by weight put into the aqueous solution; Stir every day and once make it even, the secondary fermentation surface grew white hair in 2 days; Through stirring once 2-3 days every days, foam occurs and discharge simultaneously pure fragrance again, fermentation stops to obtain fermented liquid, and this moment, sugar decomposition changed into the glucokinase activity higher alcohols; Add above-mentioned trace element by weight: first trace element is dissolved with warm water, be added in the fermented liquid and mix.
The fruit tree of the provided by the invention first preferred microorganism live bacteria rushes the method for application of fertilising, and spraying concentration is for using above-mentioned spraying of preparing after fertilizer dilutes 500-600 times.
The fruit tree of the provided by the invention second preferred microorganism live bacteria rushes the method for application of fertilising, during the sowing seed dressing the above-mentioned fertilizer that sprays for preparing is diluted 400 times of seed dressings, the rear sowing of drying in the shade.
The method of application of the fruit tree punching fertilising of the provided by the invention the 3rd preferred microorganism live bacteria is filled with root, when watering root the above-mentioned fertilizer that sprays for preparing is diluted 1000-1200 doubly, injects root.
Weight part unit is for commonly used: gram, kilogram, ton etc.; Fruit tree is suitable for apple tree, pear tree etc.
The method of application of microorganism live bacteria fruit tree punching fertilising
1, spray period: seedling stage, vegetative period, florescence, expanding stage, boot stage, filling stage are sprayed even getting final product, and decide because of the crop demand concrete period.
2, the time: before at 10 o'clock in the morning or after at 16 o'clock in afternoon, calm fine day carries out.
Brown sugar is without highly concise, the whole compositions in the sugarcane juice have almost been kept, except the function that possesses sugar, also contain VITAMIN and trace element, such as iron, zinc, manganese, chromium etc., add brown sugar and can significantly reduce the generation of dehiscent fruit phenomenon, increase substantially yield and quality, fruit face light, mouthfeel are sweet, and can ripen in advance 3-7 days, significantly improve economic benefit; Improve photosynthetic function and directly mend sugar, improve the trophic level of young fruit development phase, promote young fruit and leaf development, reduce the physiological fruit drop phenomenon and occur.
Borax: borax soln, but activated plant cell viability, promote pollen tube to grow, improve fruit-setting rate.
Sal epsom: magnesium is chlorophyllous core element, can strengthen the plant reduction process, improves resistance against diseases, promotes the activation of enzyme, makes the loosing soil that hardens strong, plants living well developed root system, fully absorbs nutrition, makes plant more luxuriant; Sulphur is to consist of the interior protein of plant materials and the main component of enzyme, can promote root growth, strengthens plant materials intracellular metabolite effect and redoxomorphism, thereby promotes that crop growth is vigorous, increases output; Contain magnesium punching fertilising than not containing rushing fertilising and can making farm crop growth 15-50% of magnesium.
Ferrous sulfate: ferro element is that the formation plant chlorophyll is necessary, and during iron deficiency, chlorophyllous formation is obstructed and is made plant generation chlorosis, and blade becomes faint yellow; Add ferrous sulfate, can improve the acidity of soil, satisfy the needs of plant-growth; Can prevent and treat the generation of yellows.Use ferrous sulfate, if in general the solution with weight part 0.1%-0.25 directly waters soil, have certain effect, but owing to the soluble iron that waters in people's soil, can be fixed to soon insoluble iron containing compounds and lost efficacy, therefore, for avoiding the loss of ferro element, can adopt the copperas solution of weight part 0.3-0.6 that plant is carried out foliage-spray.Because the movability of ferro element in plant materials be very little, therefore should be continuously to foliage-spray 3～5 times, make whole blades all can touch ferrous solution.Therefore, correctly using ferrous sulfate should be to take 0.3～0.6% the aqueous solution to carry out foliage-spray.Ferrous sulfate is soluble in water, should be used while allocating, can not be stored for a long time, and prevent its oxidation and lost efficacy.
Potassium primary phosphate is fertilizer, important growth regulator especially, its Main Function have following some: without chlorine, salt index is low, can be water-soluble.Be rich in the phosphorus potassium nutrition, safety non-toxic did not stored for a long timely lose efficacy; Can cushion the soil soda acid and change the pH value of stable soil.Can promote flowers rhizospheric microorganism flora growth and breeding.Can improve soil, make flower root system flourishing, the blade plumpness is dark green, and cane is sturdy, and disease resistance, resistance strengthen.Absorption rate can be regulated flower growth up to 80% to 90% when being used for foliage-spray, supplies nutrition at crucial moment.Potassium ion in the potassium primary phosphate and phosphate anion can directly be absorbed by flower plant, and effect is fast and obvious.
Seed soaking, seed dressing: can stimulate seed germination and promote seedling strong, make its cane sturdy, tall and straight anti-fall.Foliage application: spray the blade face in vegetative period through commonly used 0.3% potassium dihydrogen phosphate, can make branch strong full of leaves, the flower polychrome is gorgeous.Foliage-spray absorbs from leaf stoma and directly enters in the strain body, not enough to remedy the phosphorus potassium nutrition, can strengthen photosynthesis, prevent plant early ageing, strengthen Ye Zhuangen, improve the resist ability to low temperature, high temperature, promote the dynamic accumulation of starch, sugar, the formation of promotion anthocyanidin.
Manganous sulfate: can promote the growth of crop to increase output.
In addition, also any proportioning of the ratio of bacterial classification; Each raw material can the arbitrary proportion combination in above-mentioned selected component by weight.
Compared with prior art, a kind of microorganism live bacteria fruit tree provided by the invention rushes fertilising, preparation technology and method of application thereof and has the following advantages:
1, plant absorbing is fast, instant effect, and institute's fertilise namely can partly be absorbed and utilize in 20 hours, effectively reduce paying of repeatedly foliage-spray labour intensity, and application process is simple, the complex operations that does not need machinery to spread manuer in holes;
2, utilization rate of fertilizer is high, and the fertilizer of spreading manuer in holes can be not tight because covering, and the reasons such as weather arid cause fertilizer loss; Punching fertilising is water-soluble, enter underground, therefore large with the root system of plant contact surface by plant absorbing, absorb soon, specific absorption is high, has reduced because of plant to have little time to absorb the fertilizer loss that causes;
3, do not damage farm crop, machinery topdresses and can destroy the part root system of plant, affects plant to the absorption of fertilizer, also can to a certain degree affect the normal growth of plant.Machinery sprays, and people trade union causes physical abuse to Young Plant stem, sprout, young fruit, flower etc.;
4, except efficient useful living microorganism is arranged, also contain abundant organic matter and micro-; Existing pollution-free, nuisanceless, fertilizer efficiency is lasting, and strong sprout is disease-resistant, and improvement soil improves output, improves the advantages such as crop quality, can overcome again the environmental pollution that a large amount of use chemical fertilizer, agricultural chemicals bring, the drawbacks such as ecological damage;
5, refining multi-functional, the full nutrition a new generation that forms of vanguard technology contains the viable bacteria biological liquid fertilizer, this fertilizer is rich in multiple amino acids, trace element and multiple beneficial microbial metabolites, nutrition is complete, increase production high, effective, consumption is few, absorption is fast, namely safety is economical again, is to produce the ideal of green non-pollution agricultural-food with fertile.
Embodiment
By the following examples a kind of microorganism live bacteria fruit tree provided by the invention being rushed fertilising, preparation technology and method of application thereof is described in further detail;
Correlation test
Test 1: microorganism live bacteria fruit tree punching fertilising is on the impact of fruit tree
Apple tree, pear tree, peach, cherry tree, Ficus carica L. and the orange tree of selecting same experimental plot different times to plant are tested, and respectively selected fruit tree are imposed separately nitrogenous fertilizer and use the present invention to rush fertilising to compare, and comparing result is added up such as following table 1:
The blooming, bear fruit and output of table 1 fruit tree
| Project | No. of inflorescences (individual) | Inflorescence fruit-setting rate (%) | Single plant yield (Kg) |
| Apple tree single administration nitrogenous fertilizer | 746 | 32.1 | 189.6 |
| Apple tree is used the present invention and rushes fertilising | 878.042 | 41.3 | 221.8 |
| Pear tree single administration nitrogenous fertilizer | 740 | 31 | 187 |
[0057]
| Pear tree is used the present invention and rushes fertilising | 888 | 71.3 | 226.27 |
| Peach single administration nitrogenous fertilizer | 738 | 30 | 185 |
| Peach is used the present invention and rushes fertilising | 881 | 70 | 225 |
| Ficus carica L. single administration nitrogenous fertilizer | 738 | 29 | 185 |
| Ficus carica L. is used the present invention and is rushed fertilising | 885 | 70.3 | 225.7 |
| Orange fruit tree single administration nitrogenous fertilizer | 741 | 31.1 | 187.8 |
| Orange tree is used the present invention and is rushed fertilising | 880 | 71 | 225.2 |
In addition, fruit tree punching fertilising of the present invention can increase the growth of trunk, reduces the shoot growth amount, thereby obviously improves percentage of fertile fruit, sees Table 2 as an example of thornbuss example:
Table 2 percentage of fertile fruit
| Project | Dry week growth amount (cm) | Shoot growth amount (cm) | Setting quantity (Kg) |
| Single administration nitrogenous fertilizer | 46.70 | 63.80 | 106.70 |
| Use the present invention and rush fertilising | 65.6 | 43.7 | 450.6 |
Test 3: the microorganism live bacteria fruit tree punching fertilising of different components is on the impact of apple tree
The apple tree of selecting same experimental plot to plant is tested, and respectively selected apple tree is imposed the fertilizer of different components and uses the present invention to rush fertilising to compare, and comparing result is added up such as following table 3:
Table 3 percentage of fertile fruit
After used punching fertilising of the present invention is product 15 grams that embodiment 1 produces and dilutes 1000 times when above-mentioned the experiment spring fruit tree send tender shoots (vegetative period) and pour into root.
Embodiment 1
The microorganism live bacteria fruit tree punching fertilising of the present embodiment comprises the by weight following component of component meter: 30 gram brown sugar, 3 gram yeast extract pastes, 5 gram microbiobacterial agents, 0.2 gram borax, 0.2 gram sal epsom, 0.3 gram ferrous sulfate, 0.003 gram potassium primary phosphate and 0.2 gram manganous sulfate.
Microbiobacterial agent is bacterium, yeast, fungi and actinomycetes.
Bacterium is pseudomonas; Yeast is for intending the ball yeast; Fungi is viride; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
The preparation technology of the fruit tree punching fertilising of microorganism live bacteria may further comprise the steps:
1), preparation composite flora: with above-mentioned bacterium, yeast, fungi and actinomycetes by weight, and by 1: 1: 7: 2 part by weight is made composite flora;
2), spawn culture: culture dish is placed in 27.1 ℃ of incubators to cultivate can chooses bacterium in 7 days, the 3d after cultivating chooses bacterium in batches continuously, to reduce the interference of miscellaneous bacteria, selects single bacterium colony according to colonial morphology when choosing bacterium colony, and purifying is transferred to slant medium;
3), zymotechnique:
Employing distilled water dissolves into the aqueous solution by 200: 0.01 volume ratio with above-mentioned brown sugar, yeast extract paste, microbiobacterial agent, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight, when water temperature is 18 ℃ above-mentioned composite bacteria and yeast extract paste is by weight put into the aqueous solution; Stir every day and once make it even, the secondary fermentation surface grew white hair in 2 days; Through stirring once 2 day every day, foam occurs and discharge simultaneously pure fragrance again, fermentation stops to obtain fermented liquid, and this moment, sugar decomposition changed into the glucokinase activity higher alcohols; Add above-mentioned trace element by weight: first trace element is dissolved with warm water, be added in the fermented liquid and mix.
Be seedling stage, vegetative period, florescence, expanding stage, filling stage the period of spraying.Spraying concentration is for using above-mentioned spraying of preparing after fertilizer dilutes 500 times.During the sowing seed dressing the above-mentioned fertilizer that sprays for preparing is diluted 400 times of seed dressings, the rear sowing of drying in the shade.Fill with root, when watering root the above-mentioned fertilizer that sprays for preparing diluted 1000 times, inject root.
Thornbuss is bloomed improve 17.7%, fruit-setting rate improves 28.9%, single plant yield improves 18%.
Embodiment 2
The microorganism live bacteria fruit tree punching fertilising of the present embodiment comprises the by weight following component of component meter: 50 gram brown sugar, 10 gram yeast extract pastes, 10 gram microbiobacterial agents, 0.4 gram borax, 0.4 gram sal epsom, 0.6 gram ferrous sulfate, 0.006 gram potassium primary phosphate and 0.5 gram manganous sulfate.
Microbiobacterial agent is bacterium, yeast, fungi and actinomycetes.
Bacterium is the mixed bacterium that is selected from subtilis, Bacillus licheniformis, bacillus cereus; Yeast is Pichia yeast; Fungi is trichoderma harziarum; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
The preparation technology of the fruit tree punching fertilising of microorganism live bacteria may further comprise the steps:
1), preparation composite flora: with above-mentioned bacterium, yeast, fungi and actinomycetes by weight, and by 1: 5: 0.5: 2 part by weight is made composite flora;
2), spawn culture: culture dish is placed in 28.5 ℃ of incubators cultivates 6d and can choose bacterium, the 3d after cultivating chooses bacterium in batches continuously, to reduce the interference of miscellaneous bacteria, selects single bacterium colony according to colonial morphology when choosing bacterium colony, and purifying is transferred to slant medium;
3), zymotechnique:
Employing distilled water dissolves into the aqueous solution by 200: 0.1 volume ratio with above-mentioned brown sugar, yeast extract paste, microbiobacterial agent, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight, when water temperature is 22 ℃ above-mentioned composite bacteria and yeast extract paste is by weight put into the aqueous solution; Stir every day and once make it even, the secondary fermentation surface grew white hair in 2 days; Through stirring once 2 day every day, foam occurs and discharge simultaneously pure fragrance again, fermentation stops to obtain fermented liquid, and this moment, sugar decomposition changed into the glucokinase activity higher alcohols; Add above-mentioned trace element by weight: first trace element is dissolved with warm water, be added in the fermented liquid and mix.
Be seedling stage, vegetative period, florescence, expanding stage, boot stage the period of spraying, and spraying concentration is for using above-mentioned spraying of preparing after fertilizer dilutes 600 times.
During the sowing seed dressing the above-mentioned fertilizer that sprays for preparing is diluted 400 times of seed dressings, the rear sowing of drying in the shade.Fill with root, when watering root the above-mentioned fertilizer that sprays for preparing diluted 1200 times, inject root.
Grapevine is bloomed improve 19%, fruit-setting rate improves 29%, single plant yield improves 19%.
Embodiment 3
Microorganism live bacteria fruit tree punching fertilising comprises the by weight following component of component meter: 25 gram brown sugar, 5 gram yeast extract pastes, 6 gram microbiobacterial agents, 0.25 gram borax, 0.25 gram sal epsom, 0.32 gram ferrous sulfate, 0.0035 gram potassium primary phosphate and 0.22 gram manganous sulfate.
Microbiobacterial agent is bacterium, yeast, fungi and actinomycetes.
Bacterium is the mixed bacterium in the subtilis pseudomonas; Yeast is for intending the ball yeast; Fungi is trichoderma harziarum and viride; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
The preparation technology of the fruit tree punching fertilising of microorganism live bacteria may further comprise the steps:
1), preparation composite flora: with above-mentioned bacterium, yeast, fungi and actinomycetes by weight, and by 1: 5: 0.5: 2 part by weight is made composite flora;
2), spawn culture: culture dish is placed in 28.5 ℃ of incubators to cultivate can chooses bacterium in 5 days, chose continuously bacterium in the 3rd day after cultivate in batches, to reduce the interference of miscellaneous bacteria, select single bacterium colony according to colonial morphology when choosing bacterium colony, purifying is transferred to slant medium;
3), zymotechnique:
Employing distilled water dissolves into the aqueous solution by 200: 0.1 volume ratio with above-mentioned brown sugar, yeast extract paste, microbiobacterial agent, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight, when water temperature is 20 ℃ above-mentioned composite bacteria and yeast extract paste is by weight put into the aqueous solution; Stir every day and once make it even, the secondary fermentation surface grew white hair in 3 days; Through stirring once 2 day every day, foam occurs and discharge simultaneously pure fragrance again, fermentation stops to obtain fermented liquid, and this moment, sugar decomposition changed into the glucokinase activity higher alcohols; Add above-mentioned trace element by weight: first trace element is dissolved with warm water, be added in the fermented liquid and mix.
Be seedling stage, vegetative period, florescence, expanding stage, boot stage or filling stage the period of spraying.Spraying concentration is for using above-mentioned spraying of preparing after fertilizer dilutes 550 times.During the sowing seed dressing the above-mentioned fertilizer that sprays for preparing is diluted 400 times of seed dressings, the rear sowing of drying in the shade.Fill with root, when watering root the above-mentioned fertilizer that sprays for preparing diluted 1100 times, inject root.
Ficus carica L. is bloomed improve 20%, fruit-setting rate improves 28.7%, single plant yield improves 17%.
Embodiment 4
Microorganism live bacteria fruit tree punching fertilising comprises the by weight following component of component meter: 45 gram brown sugar, 9 gram yeast extract pastes, 9 gram microbiobacterial agents, 0.35 gram borax, 0.38 gram sal epsom, 0.55 gram ferrous sulfate, 0.0056 gram potassium primary phosphate and 0.47 gram manganous sulfate.
Microbiobacterial agent is bacterium, yeast, fungi and actinomycetes.
Bacterium is the mixed bacterium in subtilis, Bacillus licheniformis, the pseudomonas; Yeast be Pichia yeast and; Fungi is trichoderma harziarum and viride; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
The preparation technology of the fruit tree punching fertilising of microorganism live bacteria may further comprise the steps:
1), preparation composite flora: with above-mentioned bacterium, yeast, fungi and actinomycetes by weight, and by 1: 1: 0.5: 1 part by weight is made composite flora;
2), spawn culture: culture dish is placed in 28 ℃ of incubators to cultivate can chooses bacterium in 3 days, chose continuously bacterium in the 3rd day after cultivate in batches, to reduce the interference of miscellaneous bacteria, select single bacterium colony according to colonial morphology when choosing bacterium colony, purifying is transferred to slant medium;
3), zymotechnique:
Employing distilled water dissolves into the aqueous solution by 200: 0.1 volume ratio with above-mentioned brown sugar, yeast extract paste, microbiobacterial agent, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight, when water temperature is 20 ℃ above-mentioned composite bacteria and yeast extract paste is by weight put into the aqueous solution; Stir every day and once make it even, the secondary fermentation surface grew white hair in 2 days; Through stirring once 3 day every day, foam occurs and discharge simultaneously pure fragrance again, fermentation stops to obtain fermented liquid, and this moment, sugar decomposition changed into the glucokinase activity higher alcohols; Add above-mentioned trace element by weight: first trace element is dissolved with warm water, be added in the fermented liquid and mix.
Be seedling stage, vegetative period, florescence, expanding stage, boot stage or filling stage the period of spraying.
Spraying concentration is for using above-mentioned spraying of preparing after fertilizer dilutes 600 times.During the sowing seed dressing the above-mentioned fertilizer that sprays for preparing is diluted 400 times of seed dressings, the rear sowing of drying in the shade; Fill with root, when watering root the above-mentioned fertilizer that sprays for preparing diluted 1200 times, inject root.
Cherry tree is bloomed improve 17.7%, fruit-setting rate improves 30%, single plant yield improves 21%.
Embodiment 5
Microorganism live bacteria fruit tree punching fertilising comprises the by weight following component of component meter: 35 gram brown sugar, 8 gram yeast extract pastes, 8 gram microbiobacterial agents, 0.3 gram borax, 0.3 gram sal epsom, 0.35 gram ferrous sulfate, 0.004 gram potassium primary phosphate and 0.4 gram manganous sulfate.
Microbiobacterial agent is bacterium, yeast, fungi and actinomycetes.
Bacterium is Bacillus licheniformis; Yeast is for intending the ball yeast; Fungi is viride; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
The preparation technology of the fruit tree punching fertilising of microorganism live bacteria may further comprise the steps:
1), preparation composite flora: with above-mentioned bacterium, yeast, fungi and actinomycetes by weight, and by 1: 1: 0.5: 2 part by weight is made composite flora;
2), spawn culture: culture dish is placed in 27 ℃ of incubators to cultivate can chooses bacterium in 7 days, chose continuously bacterium in the 3rd day after cultivate in batches, to reduce the interference of miscellaneous bacteria, select single bacterium colony according to colonial morphology when choosing bacterium colony, purifying is transferred to slant medium;
3), zymotechnique:
Employing distilled water dissolves into the aqueous solution by 200: 0.08 volume ratio with above-mentioned brown sugar, yeast extract paste, microbiobacterial agent, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight, when water temperature is 21 ℃ above-mentioned composite bacteria and yeast extract paste is by weight put into the aqueous solution; Stir every day and once make it even, the secondary fermentation surface grew white hair in 2 days; Through stirring once 2 day every day, foam occurs and discharge simultaneously pure fragrance again, fermentation stops to obtain fermented liquid, and this moment, sugar decomposition changed into the glucokinase activity higher alcohols; Add above-mentioned trace element by weight: first trace element is dissolved with warm water, be added in the fermented liquid and mix.
Be seedling stage, vegetative period, florescence, expanding stage, boot stage or filling stage the period of spraying.Spraying concentration is for using above-mentioned spraying of preparing after fertilizer dilutes 600 times.The method of application of the fruit tree punching fertilising of microorganism live bacteria is diluted 400 times of seed dressings, the rear sowing of drying in the shade with the above-mentioned fertilizer that sprays for preparing when it is characterized in that sowing seed dressing.Fill with root, when watering root the above-mentioned fertilizer that sprays for preparing diluted 1000 times, inject root.
Be used for peach, peach bloomed improve 18%, fruit-setting rate improves 29%, single plant yield improves 18%.
Embodiment 6
Microorganism live bacteria fruit tree punching fertilising comprises the by weight following component of component meter: 40 gram brown sugar, 7 gram yeast extract pastes, 7 gram microbiobacterial agents, 0.32 gram borax, 0.33 gram sal epsom, 0.5 gram ferrous sulfate, 0.005 gram potassium primary phosphate and 0.28 gram manganous sulfate.
Microbiobacterial agent is bacterium, yeast, fungi and actinomycetes.
Bacterium is subtilis; Yeast is for intending the ball yeast; Fungi is viride; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
The preparation technology of the fruit tree punching fertilising of microorganism live bacteria may further comprise the steps:
1), preparation composite flora: with above-mentioned bacterium, yeast, fungi and actinomycetes by weight, and by 1: 1: 0.5: 1 part by weight is made composite flora;
2), spawn culture: culture dish is placed in 27.5 ℃ of incubators to cultivate can chooses bacterium in 5 days, chose continuously bacterium in the 3rd day after cultivate in batches, to reduce the interference of miscellaneous bacteria, select single bacterium colony according to colonial morphology when choosing bacterium colony, purifying is transferred to slant medium;
3), zymotechnique:
Adopt distilled water by 200: the volume ratio of 0.01-0.1 dissolves into the aqueous solution with above-mentioned brown sugar, yeast extract paste, microbiobacterial agent, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight, when water temperature is 21.5 ℃ above-mentioned composite bacteria and yeast extract paste is by weight put into the aqueous solution; Stir every day and once make it even, the secondary fermentation surface grew white hair in 3 days; Through stirring once 3 day every day, foam occurs and discharge simultaneously pure fragrance again, fermentation stops to obtain fermented liquid, and this moment, sugar decomposition changed into the glucokinase activity higher alcohols; Add above-mentioned trace element by weight: first trace element is dissolved with warm water, be added in the fermented liquid and mix.
Be seedling stage, vegetative period, florescence, expanding stage, boot stage or filling stage the period of spraying.Spraying concentration is for using above-mentioned spraying of preparing after fertilizer dilutes 540 times.During the sowing seed dressing the above-mentioned fertilizer that sprays for preparing is diluted 400 times of seed dressings, the rear sowing of drying in the shade.Fill with root, when watering root the above-mentioned fertilizer that sprays for preparing diluted 1000 times, inject root.
The fertilizer for preparing is used for orange tree, orange tree is bloomed improve 17.7-20%, fruit-setting rate raising 28.7-30%, single plant yield raising 17-21%.
Embodiment 7
Microorganism live bacteria fruit tree punching fertilising comprises the by weight following component of component meter: 30 gram brown sugar, 7 gram yeast extract pastes, 7 gram microbiobacterial agents, 0.28 gram borax, 0.3 gram sal epsom, 0.4 gram ferrous sulfate, 0.004 gram potassium primary phosphate and 0.3 gram manganous sulfate.
Microbiobacterial agent is bacterium, yeast, fungi and actinomycetes.
Bacterium is the mixed bacterium that is selected from subtilis, Bacillus licheniformis, bacillus cereus; Yeast is Pichia yeast and intends the ball yeast; Fungi is trichoderma harziarum and viride; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are CGMCC No 4920; The preservation name is called POLO-BLN1103.
The preparation technology of the fruit tree punching fertilising of microorganism live bacteria may further comprise the steps:
1), preparation composite flora: with above-mentioned bacterium, yeast, fungi and actinomycetes by weight, and by 2: 5: 7: 2 part by weight is made composite flora;
2), spawn culture: culture dish is placed in 27.1 ℃ of incubators to cultivate can chooses bacterium in 4 days, chose continuously bacterium in the 3rd day after cultivate in batches, to reduce the interference of miscellaneous bacteria, select single bacterium colony according to colonial morphology when choosing bacterium colony, purifying is transferred to slant medium;
3), zymotechnique:
Adopt distilled water by 200: the volume ratio of 0.01-0.1 dissolves into the aqueous solution with above-mentioned brown sugar, yeast extract paste, microbiobacterial agent, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight, when water temperature is 22 ℃ above-mentioned composite bacteria and yeast extract paste is by weight put into the aqueous solution; Stir every day and once make it even, the secondary fermentation surface grew white hair in 2 days; Through stirring once 3 day every day, foam occurs and discharge simultaneously pure fragrance again, fermentation stops to obtain fermented liquid, and this moment, sugar decomposition changed into the glucokinase activity higher alcohols; Add above-mentioned trace element by weight: first trace element is dissolved with warm water, be added in the fermented liquid and mix.
Be seedling stage, vegetative period, florescence, expanding stage, boot stage or filling stage the period of spraying, and spraying concentration is for using above-mentioned spraying of preparing after fertilizer dilutes 580 times; During the sowing seed dressing the above-mentioned fertilizer that sprays for preparing is diluted 400 times of seed dressings, the rear sowing of drying in the shade.Fill with root, when watering root the above-mentioned fertilizer that sprays for preparing diluted 1200 times, inject root.
The fertilizer for preparing is used for pear tree, pear tree is bloomed improve 20%, fruit-setting rate improves 30%, single plant yield improves 21%.
Embodiment 8
Microorganism live bacteria fruit tree punching fertilising comprises the by weight following component of component meter: 40 gram brown sugar, 8 gram yeast extract pastes, 8 gram microbiobacterial agents, 0.3 gram borax, 0.35 gram sal epsom, 0.5 gram ferrous sulfate, 0.005 gram potassium primary phosphate and 0.4 gram manganous sulfate.
Microbiobacterial agent is bacterium, yeast, fungi and actinomycetes.
Bacterium is the mixed bacterium of subtilis, Bacillus licheniformis, bacillus cereus, pseudomonas; Described yeast is Pichia yeast; Fungi is trichoderma harziarum; Actinomycetes are Streptomycin sulphate.
Bacillus licheniformis, its deposit number are C6MCC No 4920; The preservation name is called POLO-BLN1103.
The preparation technology of the fruit tree punching fertilising of microorganism live bacteria may further comprise the steps:
1), preparation composite flora: with above-mentioned bacterium, yeast, fungi and actinomycetes by weight, and by 1.5: 3: 5: 1.5 part by weight is made composite flora;
2), spawn culture: culture dish is placed in 27.8 ℃ of incubators to cultivate can chooses bacterium in 4 days, chose continuously bacterium in the 3rd day after cultivate in batches, to reduce the interference of miscellaneous bacteria, select single bacterium colony according to colonial morphology when choosing bacterium colony, purifying is transferred to slant medium;
3), zymotechnique:
Employing distilled water dissolves into the aqueous solution by 200: 0.08 volume ratio with above-mentioned brown sugar, yeast extract paste, microbiobacterial agent, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight, when water temperature is 19 ℃ above-mentioned composite bacteria and yeast extract paste is by weight put into the aqueous solution; Stir every day and once make it even, the secondary fermentation surface grew white hair in 3 days; Through stirring once 3 day every day, foam occurs and discharge simultaneously pure fragrance again, fermentation stops to obtain fermented liquid, and this moment, sugar decomposition changed into the glucokinase activity higher alcohols; Add above-mentioned trace element by weight: first trace element is dissolved with warm water, be added in the fermented liquid and mix.
Be seedling stage, vegetative period, florescence, expanding stage, boot stage or filling stage the period of spraying.
Spraying concentration is for using above-mentioned spraying of preparing after fertilizer dilutes 600 times; During the sowing seed dressing the above-mentioned fertilizer that sprays for preparing is diluted 400 times of seed dressings, the rear sowing of drying in the shade.Fill with root, when watering root the above-mentioned fertilizer that sprays for preparing diluted 1150 times, inject root.
The fertilizer for preparing is used for apple tree, apple tree is bloomed improve 17.7%, fruit-setting rate improves 28.7%, single plant yield improves 17%.
Above embodiment is only in order to illustrate that technical scheme of the present invention is not intended to limit; after although those skilled in the art reads the application; with reference to above-described embodiment the present invention is carried out various modifications or change; but these modifications or change are all within right application requirement protection domain of the present invention is awaited the reply in application.
Claims (11)
1. a microorganism live bacteria fruit tree punching fertilising is characterized in that comprising the by weight following component of component meter: 25-50 part brown sugar, 3-10 part yeast extract paste, 5-10 part microbiobacterial agent, 0.2-0.4 part borax, 0.2-0.4 part sal epsom, 0.3-0.6 part ferrous sulfate, 0.003-0.006 part potassium primary phosphate and 0.2-0.5 part manganous sulfate.
2. microorganism live bacteria fruit tree punching fertilising according to claim 1 is characterized in that comprising the by weight following component of component meter: 25-45 part brown sugar, 5-9 part yeast extract paste, 6-9 part microbiobacterial agent, 0.25-0.35 part borax, 0.25-0.38 part sal epsom, 0.32-0.55 part ferrous sulfate, 0.0035-0.0056 part potassium primary phosphate and 0.22-0.47 part manganous sulfate.
3. microorganism live bacteria fruit tree punching fertilising according to claim 1 is characterized in that comprising the by weight following component of component meter: 30-40 part brown sugar, 7-8 part yeast extract paste, 7-8 part microbiobacterial agent, 0.28-0.3 part borax, 0.3-0.35 part sal epsom, 0.4-0.5 part ferrous sulfate, 0.004-0.005 part potassium primary phosphate and 0.3-0.4 part manganous sulfate.
4. arbitrary described microorganism live bacteria fruit tree punching fertilising according to claim 1-3 is characterized in that described microbiobacterial agent is bacterium, yeast, fungi and actinomycetes.
5. microorganism live bacteria fruit tree according to claim 3 punching fertilising is characterized in that described bacterium is one or more the mixed bacterium that is selected from subtilis, Bacillus licheniformis, bacillus cereus, the pseudomonas; Described yeast is Pichia yeast and/or intends the ball yeast; Described fungi is trichoderma harziarum and/or viride; Described actinomycetes are Streptomycin sulphate.
6. Bacillus licheniformis claimed in claim 3, its deposit number is CGMCC No 4920; The preservation name is called POLO-BLN1103.
7. the fruit tree of arbitrary described microorganism live bacteria of a claim 1-3 rushes the preparation technology of fertilising, may further comprise the steps:
1), preparation composite flora: with above-mentioned bacterium, yeast, fungi and actinomycetes by weight, and press (1-2): (1-5): (0.5-7): part by weight (1-2) is made composite flora;
2), spawn culture: culture dish is placed in the 27.1-28.5 ℃ of incubator to cultivate chose bacterium in 3-7 days, bacterium is chosen in after cultivate the 3rd day in batches continuously, to reduce the interference of miscellaneous bacteria, select single bacterium colony according to colonial morphology when choosing bacterium colony, purifying is transferred to slant medium;
3), zymotechnique:
Adopt distilled water by 200: the volume ratio of 0.01-0.1 dissolves into the aqueous solution with above-mentioned brown sugar, yeast extract paste, microbiobacterial agent, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight, when water temperature is 18-22 ℃ above-mentioned composite bacteria and yeast extract paste is by weight put into the aqueous solution; Stir every day and once make it even, the secondary fermentation surface grew white hair in 2-3 days; Through stirring once 2-3 days every days, foam occurs and discharge simultaneously pure fragrance again, fermentation stops to obtain fermented liquid, and this moment, sugar decomposition changed into the glucokinase activity higher alcohols; Add above-mentioned trace element by weight: first trace element is dissolved with warm water, be added in the fermented liquid and mix.
8. the fruit tree of microorganism live bacteria according to claim 5 rushes the method for application of fertilising, it is characterized in that:
Spawn culture: culture dish is placed in 28 ℃ of incubators to cultivate can chooses bacterium in 3 days, chose continuously bacterium in the 3rd day after cultivate in batches, to reduce the interference of miscellaneous bacteria, select single bacterium colony according to colonial morphology when choosing bacterium colony, purifying is transferred to slant medium;
Zymotechnique:
Adopt distilled water by 200: the volume ratio of 0.01-0.1 dissolves into the aqueous solution with above-mentioned brown sugar, yeast extract paste, microbiobacterial agent, borax, sal epsom, ferrous sulfate, potassium primary phosphate and manganous sulfate by weight, when water temperature is 20 ℃ above-mentioned composite bacteria and yeast extract paste is by weight put into the aqueous solution; Stir every day and once make it even, the secondary fermentation surface grew white hair in 2 days; Through stirring once 2-3 days every days, foam occurs and discharge simultaneously pure fragrance again, fermentation stops to obtain fermented liquid, and this moment, sugar decomposition changed into the glucokinase activity higher alcohols; Add above-mentioned trace element by weight: first trace element is dissolved with warm water, be added in the fermented liquid and mix.
9. the method for application of the fruit tree of microorganism live bacteria claimed in claim 8 punching fertilising is characterized in that spraying concentration is that above-mentioned spraying of preparing used after fertilizer dilutes 500-600 times.
10. the method for application of the fruit tree of microorganism live bacteria claimed in claim 8 punching fertilising is diluted 400 times of seed dressings, the rear sowing of drying in the shade with the above-mentioned fertilizer that sprays for preparing when it is characterized in that sowing seed dressing.
11. the method for application of the fruit tree of microorganism live bacteria claimed in claim 8 punching fertilising is characterized in that filling with root, when watering root the above-mentioned fertilizer that sprays for preparing is diluted 1000-1200 doubly, injects root.
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