CN103274834B - Special insecticidal sterilizing microbial fertilizer for garlic and preparation method thereof - Google Patents
Special insecticidal sterilizing microbial fertilizer for garlic and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a special insecticidal sterilizing microbial fertilizer for garlic and a preparation method thereof. The special insecticidal sterilizing microbial fertilizer for the garlic comprises the raw materials in percentage by weight as follows: 8-12 parts of potassium chloride, 0.2-1.0 part of manganese sulfate, 1-3 parts of zinc sulfate, 20-25 parts of grass carbon, 5-15 parts of fowl manure, 4-6 parts of chicken slaughter house waste, 16-18 parts of stalks, 10-12 parts of residue of monosodium glutamate, 18-20 parts of soya bean meal powder, 0.3-0.5 parts of bacillus amyloliquefaciens, 0.2-0.4 parts of bacillus subtilis, 0.4-0.6 parts of jelly-shaped bacillus and the like. With the adoption of the fertilizer, the yield range of young garlic shoot and garlic is larger, the quality is improved obviously, and deinsectization, sterilization, low toxicity and pollution-free performance are achieved.
Description
Technical field
The present invention relates to the degerming microbial fertilizer of a kind of desinsection, be specifically related to degerming microbial fertilizer of a kind of garlic special pesticide and preparation method thereof, belong to fertilizer field.
Background technology
Garlic has the effect of antisepsis and anti-inflammation, and can protect liver, regulate blood sugar, protect cardiovascular, lipidemia and arteriosclerosis, anti-platelet aggregation, therefore has a very important role to the mankind.National Cancer Institute is advancing one to be intended to improve national food habits, cancer morbidity is reduced " plan of design food " of half.In the pyramid structure figure of the likely important foodstuffs of preventing cancer, garlic ranks top, namely the most effective.Therefore the plantation of garlic also more and more comes into one's own.Garlic root distribution is shallow, and root hair is few, and fertilizer sucting ability is weak, requires higher to the quality and quantity of base manure.Owing to advocating Organic farming now, therefore the fertilising that organises of garlic is also very necessary.But at present traditional common chemical fertilizer fertilising also being rested on to the fertilising of garlic, the Garlic yield produced and quality are not high.Because adding that vegetable grower applies these chemical fertilizer and do not consider that the nutrient quantity of local soil nutrient supply situation and Crop is to determine ratio, both contaminated soil environment, limit again the yield and quality of crop, to crop, soil lacks specific aim, fertilizer efficiency utilization ratio is low, also large to environmental hazard, rivers and underground water eutrophication are on the rise, soil organic matter content reduces, in soil, beneficial microorganism living space diminishes, quantity reduces, farm crop disease-resistant, cold-resistant, drought-resistant ability reduces, the generation of disease and pest is more and more serious, Garlic quality is caused to decline, output reduces.
Microbial fertilizer as a kind of important agrotechnical measure development an agriculture featuring high yields, fine quality and high efficiency in effect more and more be familiar with by people, this provides sizable development space to its exploitation, popularization, application, particularly at Development of Organic Agriculture, the irreplaceability produced in A level, AA level green food, fully demonstrate its potential glamour.There is chemical fertilizer and use the problems such as excessive, specific aim is poor, nutrient is unbalanced, existing microbial fertilizer application effect is unstable in the production of current garlic, and also rarely has report to the report of the degerming microbial fertilizer of garlic special pesticide at present.
Summary of the invention
The present invention, mainly for the deficiencies in the prior art, provides degerming microbial fertilizer of a kind of garlic special pesticide and preparation method thereof, can improve beneficial microorganism content in soil, reduces disease and pest, improves the yield and quality of garlic.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
The degerming microbial fertilizer of a kind of garlic special pesticide, it is characterized in that, the raw material of the degerming fertilizer of described desinsection is made up of the composition of following weight ratio: Repone K 8-12 part, manganous sulfate 0.2-1.0 part, zinc sulfate 1-3 part, peat composed of rotten mosses 20-25 part, chicken manure 5-15 part, chicken slaughterhouse waste 4-6 part, waste of flax 16-18 part, residue of monosodium glutamate 10-12 part, bean cake powder 18-20 part, bacillus amyloliquefaciens 0.3-0.5 part, subtilis 0.2-0.4 part, bacillusmusilaginosiengineering 0.4-0.6 part, bacstearothermophilus 0.8-1 part, D-lactic acid genus bacillus 0.2-0.4 part, aspergillus oryzae 0.9-1.1 part, hemicellulase 0.5-0.7 part, stover ferment agent 1.2-1.4 part, Chinese prickly ash 20-30 part, ginkgo seed coat 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, matrine 5-10 part, Seed of Chinese Soapberry exosper 55-60 part.
The preparation method of the degerming microbial fertilizer of garlic special pesticide, it comprises the following steps:
(1) fermentation culture of microorganism:
1. be inoculated on the inclined-plane of PDA substratum by bacillus amyloliquefaciens, subtilis, bacillusmusilaginosiengineering, the initial pH of substratum controls at 6-8; Culture temperature controls at 25-35 DEG C; Cultivate and make bacteria suspension after 15-17 hour, more in proportion 1% inoculum size by the bacterial suspension inoculation for preparing in PDA liquid nutrient medium, concussion is cultivated, and temperature controls at 28-32 DEG C, cultivates 50-70 hour;
2. bacstearothermophilus, D-lactic acid genus bacillus are being improved on Camp-BAP substratum, the initial pH of substratum controls at 6-8, temperature is 15-20 DEG C, cultivate 16-18 days, increase the temperature to 22-24 DEG C to continue to cultivate 12-16 days, the inoculum size of 1.5% is inoculated in improvement Camp-BAP liquid nutrient medium and ferments in proportion again, at 25-30 DEG C of temperature, cultivate 80-90 hour;
(2) process of crop material:
Waste of flax is spread out airing to moisture content below 10 parts; Then smashed in cutting machine internal cutting by above-mentioned stalk, the length after cutting is 2-3cm;
(3) fermentative processing:
1. bacillus amyloliquefaciens, subtilis, bacillusmusilaginosiengineering, hemicellulase, stover ferment agent are mixed according to described ratio, add water and stir, join in the mixture of the peat composed of rotten mosses, waste of flax, residue of monosodium glutamate, bean cake powder, mix and carry out fermentative processing, by mixture covered rearing with plastic film, become thoroughly decomposed time 2-3 days, when discovery temperature is more than 60 DEG C, open film and mixture carried out overturning, the timed interval be 1-2 days once, fermented through 8-10 days;
2. bacstearothermophilus, D-lactic acid genus bacillus, aspergillus oryzae are joined in chicken manure and chicken slaughterhouse waste, throwing is turned in mechanize, with opening material, increase amount of oxygen, cultivate after 1-2 days, by mixture covered rearing with plastic film, become thoroughly decomposed time 3-4 days, open film and mixture carried out overturning, the timed interval be 1-2 days once, overturning 2-4 time, and then by the process of plastics film jack, every square metre of slotting 15-25 1-2cm
2sky, suitably strengthen amount of oxygen, temperature controls at 50-55 DEG C, when discovery material loosens, does not have the stink that material is original, and slightly ammonia taste, ferments complete, obtain the fermented product that moisture content is 35-40 part when producing white hypha in heap;
(4) Repone K, manganous sulfate, zinc sulfate, Chinese prickly ash, ginkgo seed coat, Fructus Cnidii, Cortex Meliae, Rangooncreeper Fruit, Turkey-galls, Root of Pubescent Holly, matrine, Seed of Chinese Soapberry exosper are mixed, obtained mixed fertilizer;
(5) material that step (3) and step (4) obtain is mixed, stir;
(6) dry, pulverize, make finished product: dried through fluidized-bed by above-mentioned material, temperature controls below 80 DEG C of degree, and water content control, within the scope of 10-15%, pulverizes and sieves, and fineness reaches 60 orders; Then send into roller pelletizer after crushed with travelling belt, in cylinder, uperize makes heating material to 50-60 DEG C, makes material balling-up by cylinder not stall is dynamic; Then drying roller is sent in balling-up, input hot-air seasoning, then cool, sieve, canned one-tenth finished product.
Described stover ferment agent buys the stover ferment agent produced from agriculture Citroen zx bio tech ltd.
The invention has the beneficial effects as follows:
(1) the present invention adds bacillus amyloliquefaciens, can reduce the generation of anthrax in being manured into soil, and effectively can suppress the generation of gray mold; Contained subtilis has secretion organic acid function, and make garlic rhizosphere soil acidification, chelating phosphorus potassium, reduces phosphorus potassium fixation, and then reduces fertilizer amount, and Term Fertilization balances more in addition, improves fertilizer utilization efficiency;
(2) fertilizer product of the present invention's acquisition, the materials such as the stalk utilizing microorganism species fermentative processing to cross and ight soil have passed through the stage of becoming thoroughly decomposed fully, there is thorough, sufficient feature of fermenting of becoming thoroughly decomposed, and the microorganism species produced can continue breeding in being manured into soil, can coexist with original probiotic bacterium, do not affect the pathogenic micro-organism of plant growth, worm's ovum etc., environmental sound;
(3) decrease the consumption of conventional macroelement, reduce the environmental risk that chemical fertilizer loss is brought;
(4) of the present inventionly can be used for broadcasting soil treatment before rear seedling, prevent and kill off the sterilization idiocratic of farmland weed and wide spectrum.Compared with prior art, it is good, environmentally safe that distinctive formula synergy formulation of the present invention has herbicidal effect, and weeds such as not easily to develop immunity to drugs at the feature, have a extensive future.To people and animals, relatively safety is to eyes, no skin irritation in bactericidal and herbicidal agent of the present invention, and be low toxicity through day skin test checking its acute toxicity bright, easily decompose in air and soil, non-poisonous material remains; The present invention's seed dressing is little on the impact of farmland natural enemy, contributes to keeping ecotope, more friendly to environment, meets the production requirement of crop.
Embodiment
Below preferred embodiment of the present invention is described in detail, can be easier to make advantages and features of the invention be readily appreciated by one skilled in the art, thus more explicit defining is made to protection scope of the present invention.
embodiment 1
The degerming microbial fertilizer of a kind of garlic special pesticide, it is characterized in that, the raw material of the degerming fertilizer of described desinsection is made up of the composition of following weight ratio: Repone K 8-12 part, manganous sulfate 0.2-1.0 part, zinc sulfate 1-3 part, peat composed of rotten mosses 20-25 part, chicken manure 5-15 part, chicken slaughterhouse waste 4-6 part, waste of flax 16-18 part, residue of monosodium glutamate 10-12 part, bean cake powder 18-20 part, bacillus amyloliquefaciens 0.3-0.5 part, subtilis 0.2-0.4 part, bacillusmusilaginosiengineering 0.4-0.6 part, bacstearothermophilus 0.8-1 part, D-lactic acid genus bacillus 0.2-0.4 part, aspergillus oryzae 0.9-1.1 part, hemicellulase 0.5-0.7 part, stover ferment agent 1.2-1.4 part, Chinese prickly ash 20-30 part, ginkgo seed coat 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, matrine 5-10 part, Seed of Chinese Soapberry exosper 55-60 part.
The preparation method of the degerming microbial fertilizer of garlic special pesticide, is characterized in that comprising the following steps:
(1) fermentation culture of microorganism:
1. be inoculated on the inclined-plane of PDA substratum by bacillus amyloliquefaciens, subtilis, bacillusmusilaginosiengineering, the initial pH of substratum controls at 6-8; Culture temperature controls at 25-35 DEG C; Cultivate and make bacteria suspension after 15-17 hour, more in proportion 1% inoculum size by the bacterial suspension inoculation for preparing in PDA liquid nutrient medium, concussion is cultivated, and temperature controls at 28-32 DEG C, cultivates 50-70 hour;
2. bacstearothermophilus, D-lactic acid genus bacillus are being improved on Camp-BAP substratum, the initial pH of substratum controls at 6-8, temperature is 15-20 DEG C, cultivate 16-18 days, increase the temperature to 22-24 DEG C to continue to cultivate 12-16 days, the inoculum size of 1.5% is inoculated in improvement Camp-BAP liquid nutrient medium and ferments in proportion again, at 25-30 DEG C of temperature, cultivate 80-90 hour;
(2) process of crop material:
Waste of flax is spread out airing to moisture content below 10 parts; Then smashed in cutting machine internal cutting by above-mentioned stalk, the length after cutting is 2-3cm;
(3) fermentative processing:
1. bacillus amyloliquefaciens, subtilis, bacillusmusilaginosiengineering, hemicellulase, stover ferment agent are mixed according to described ratio, add water and stir, join in the mixture of the peat composed of rotten mosses, waste of flax, residue of monosodium glutamate, bean cake powder, mix and carry out fermentative processing, by mixture covered rearing with plastic film, become thoroughly decomposed time 2-3 days, when discovery temperature is more than 60 DEG C, open film and mixture carried out overturning, the timed interval be 1-2 days once, fermented through 8-10 days;
2. bacstearothermophilus, D-lactic acid genus bacillus, aspergillus oryzae are joined in chicken manure and chicken slaughterhouse waste, throwing is turned in mechanize, with opening material, increase amount of oxygen, cultivate after 1-2 days, by mixture covered rearing with plastic film, become thoroughly decomposed time 3-4 days, open film and mixture carried out overturning, the timed interval be 1-2 days once, overturning 2-4 time, and then by the process of plastics film jack, every square metre of slotting 15-25 1-2cm
2sky, suitably strengthen amount of oxygen, temperature controls at 50-55 DEG C, when discovery material loosens, does not have the stink that material is original, and slightly ammonia taste, ferments complete, obtain the fermented product that moisture content is 35-40 part when producing white hypha in heap;
(4) Repone K, manganous sulfate, zinc sulfate, Chinese prickly ash, ginkgo seed coat, Fructus Cnidii, Cortex Meliae, Rangooncreeper Fruit, Turkey-galls, Root of Pubescent Holly, matrine, Seed of Chinese Soapberry exosper are mixed, obtained mixed fertilizer;
(5) material that step (3) and step (4) obtain is mixed, stir;
(6) dry, pulverize, make finished product: dried through fluidized-bed by above-mentioned material, temperature controls below 80 DEG C of degree, and water content control, within the scope of 10-15%, pulverizes and sieves, and fineness reaches 60 orders; Then send into roller pelletizer after crushed with travelling belt, in cylinder, uperize makes heating material to 50-60 DEG C, makes material balling-up by cylinder not stall is dynamic; Then drying roller is sent in balling-up, input hot-air seasoning, then cool, sieve, canned one-tenth finished product.
Microbial fertilizer prepared by table 1 the present invention and contrast 1 are compared
| Garlic stems increment (part) | Garlic increment (part) | |
| Embodiment 1 | 11 | 14 |
The degerming fertilizer of the desinsection (contrast 2 that the degerming microbial fertilizer of desinsection embodiment 1 obtained and Qufu City Jin Shili science and technology Fei Ye company limited produce, its total nutrient >=45 part, N-P-K is 15-15-15) be applied to Jinxiang hybridization garlic respectively, garlic is unified plantation, outdoor cropping, unified management; Embodiment is identical with the fertilizer amount of contrast 2.Respectively its garlic stems output, Garlic yield contrast are compared, obtain its comparing result.Concrete as table 2:
Microbial fertilizer prepared by table 2 the present invention and contrast 2 are compared
| Garlic stems increment (part) | Garlic increment (part) | |
| Embodiment 1 | 15 | 20 |
Can find from above contrast, the present invention compares with ordinary compound fertilizer with common bio-organic fertilizer, and amount of increase in production is comparatively large, and oil recovery enhancement is obvious, garlic stems rate of putting forth is high, and output is high, and color and luster is bud green, and quality is tender and crisp, a kind of sedge bud does not expand, without deformity and mildew and rot phenomenon, and even thickness, complete submissive; A kind of sedge base length is greater than 32 centimetres.The garlic head of garlic mean diameter using fertilizer of the present invention is 5.32 centimetres, average substance 179 grams; Use the garlic of common fertilizer, head of garlic mean diameter is 4.29 centimetres, average substance 140 grams, and the ultimate production of the head of garlic increases; Seedling rate of the present invention on average reaches 82%, and contrast treatment reaches conspicuous level, is 75.6% to the inhibiting rate of the common disease of potato.
Of the present invention through field comparison test, all obtain positive effect, main manifestations is as follows: containing abundant organic and humic acid, can improve Soil structure, vegetable soil, promote that soil is built up fertility.With other fertilizer ratio such as nutrient such as grade, crop yield 21%.Formulate and coordinate farm crop and absorb nutrition, stimulate and regulation and control plant growth, reduce or reduce crop pest, affecting crop quality.Or fertilizer amount need not be reduced, be beneficial to protection of the environment.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.
Claims (1)
1. the degerming microbial fertilizer of garlic special pesticide, it is characterized in that, the raw material of the degerming fertilizer of described desinsection is made up of the composition of following weight ratio: Repone K 8-12 part, manganous sulfate 0.2-1.0 part, zinc sulfate 1-3 part, peat composed of rotten mosses 20-25 part, chicken manure 5-15 part, chicken slaughterhouse waste 4-6 part, waste of flax 16-18 part, residue of monosodium glutamate 10-12 part, bean cake powder 18-20 part, bacillus amyloliquefaciens 0.3-0.5 part, subtilis 0.2-0.4 part, bacillusmusilaginosiengineering 0.4-0.6 part, bacstearothermophilus 0.8-1 part, D-lactic acid genus bacillus 0.2-0.4 part, aspergillus oryzae 0.9-1.1 part, hemicellulase 0.5-0.7 part, stover ferment agent 1.2-1.4 part, Chinese prickly ash 20-30 part, ginkgo seed coat 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, matrine 5-10 part, Seed of Chinese Soapberry exosper 55-60 part, being prepared by following preparation method of the degerming microbial fertilizer of described desinsection:
(1) fermentation culture of microorganism:
1. be inoculated on the inclined-plane of PDA substratum by bacillus amyloliquefaciens, subtilis, bacillusmusilaginosiengineering, the initial pH of substratum controls at 6-8; Culture temperature controls at 25-35 DEG C; Cultivate and make bacteria suspension after 15-17 hour, more in proportion 1% inoculum size by the bacterial suspension inoculation for preparing in PDA liquid nutrient medium, concussion is cultivated, and temperature controls at 28-32 DEG C, cultivates 50-70 hour;
2. bacstearothermophilus, D-lactic acid genus bacillus are being improved on Camp-BAP substratum, the initial pH of substratum controls at 6-8, temperature is 15-20 DEG C, cultivate 16-18 days, increase the temperature to 22-24 DEG C to continue to cultivate 12-16 days, the inoculum size of 1.5% is inoculated in improvement Camp-BAP liquid nutrient medium and ferments in proportion again, at 25-30 DEG C of temperature, cultivate 80-90 hour;
(2) process of crop material:
Waste of flax is spread out airing to moisture content below 10 parts; Then smashed in cutting machine internal cutting by above-mentioned stalk, the length after cutting is 2-3cm;
(3) fermentative processing:
1. bacillus amyloliquefaciens, subtilis, bacillusmusilaginosiengineering, hemicellulase, stover ferment agent are mixed according to described ratio, add water and stir, join in the mixture of the peat composed of rotten mosses, waste of flax, residue of monosodium glutamate, bean cake powder, mix and carry out fermentative processing, by mixture covered rearing with plastic film, become thoroughly decomposed time 2-3 days, when discovery temperature is more than 60 DEG C, open film and mixture carried out overturning, the timed interval be 1-2 days once, fermented through 8-10 days;
2. bacstearothermophilus, D-lactic acid genus bacillus, aspergillus oryzae are joined in chicken manure and chicken slaughterhouse waste, throwing is turned in mechanize, with opening material, increase amount of oxygen, cultivate after 1-2 days, by mixture covered rearing with plastic film, become thoroughly decomposed time 3-4 days, open film and mixture carried out overturning, the timed interval be 1-2 days once, overturning 2-4 time, and then by the process of plastics film jack, every square metre of slotting 15-25 1-2cm
2sky, suitably strengthen amount of oxygen, temperature controls at 50-55 DEG C, when discovery material loosens, does not have the stink that material is original, and slightly ammonia taste, ferments complete, obtain the fermented product that moisture content is 35-40 part when producing white hypha in heap;
(4) Repone K, manganous sulfate, zinc sulfate, Chinese prickly ash, ginkgo seed coat, Fructus Cnidii, Cortex Meliae, Rangooncreeper Fruit, Turkey-galls, Root of Pubescent Holly, matrine, Seed of Chinese Soapberry exosper are mixed, obtained mixed fertilizer;
(5) material that step (3) and step (4) obtain is mixed, stir;
(6) dry, pulverize, make finished product: dried through fluidized-bed by above-mentioned material, temperature controls below 80 DEG C of degree, and water content control, within the scope of 10-15%, pulverizes and sieves, and fineness reaches 60 orders; Then send into roller pelletizer after crushed with travelling belt, in cylinder, uperize makes heating material to 50-60 DEG C, makes material balling-up by cylinder not stall is dynamic; Then drying roller is sent in balling-up, input hot-air seasoning, then cool, sieve, canned one-tenth finished product.
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| CN103613448A (en) * | 2013-11-08 | 2014-03-05 | 苏州仁成生物科技有限公司 | Chinese herbal medicine polyelement green fertilizer and preparation method thereof |
| CN104496573B (en) * | 2014-12-04 | 2018-02-09 | 上海绿乐生物科技有限公司 | A kind of grape special biological and ecological methods to prevent plant disease, pests, and erosion composite microbiological fertilizer, production method and application |
| CN105198556A (en) * | 2015-08-17 | 2015-12-30 | 苏州玖沃生物科技有限公司 | Microbial fertilizer capable of improving sweetness of oranges and preparation method thereof |
| CN108841766A (en) * | 2018-07-27 | 2018-11-20 | 苏州汉德瑞生物工程有限公司 | A kind of preparation method of the fresh-keeping bacteria strain of microorganism |
| CN112830825A (en) * | 2019-11-04 | 2021-05-25 | 湖南满师傅食品有限公司 | Bean dreg biofertilizer technology |
| CN110951644B (en) * | 2019-12-23 | 2021-12-10 | 河南新仰韶生物科技有限公司 | Garlic composite microbial agent containing multiple microorganisms and preparation method thereof |
| CN111205144A (en) * | 2020-03-25 | 2020-05-29 | 杭州迹童精密机械有限公司 | Microbial fertilizer and preparation method thereof |
| CN115466150A (en) * | 2022-09-14 | 2022-12-13 | 辽宁拓展农业发展有限公司 | Fertilizer containing plant for expelling insects and sterilizing and preparation method thereof |
| CN115725470A (en) * | 2022-11-29 | 2023-03-03 | 河南康盈环保科技有限公司 | Preparation method and application of garlic planting soil improvement microbial inoculum |
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| CN1785938A (en) * | 2005-10-31 | 2006-06-14 | 阎成谦 | Plant composite insecticidal fertilizer and its production technology |
| CN101580420A (en) * | 2008-05-15 | 2009-11-18 | 周广辉 | Preparation method of garlic yield-increasing fertilizer |
| CN102010255A (en) * | 2009-09-08 | 2011-04-13 | 周广辉 | Garlic yield-increasing fertilizer |
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| CN1304912A (en) * | 2000-12-31 | 2001-07-25 | 李承海 | Process for preparing microecological organic fertilizer |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1785938A (en) * | 2005-10-31 | 2006-06-14 | 阎成谦 | Plant composite insecticidal fertilizer and its production technology |
| CN101580420A (en) * | 2008-05-15 | 2009-11-18 | 周广辉 | Preparation method of garlic yield-increasing fertilizer |
| CN102010255A (en) * | 2009-09-08 | 2011-04-13 | 周广辉 | Garlic yield-increasing fertilizer |
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