CN112830825A - Bean dreg biofertilizer technology - Google Patents
Bean dreg biofertilizer technology Download PDFInfo
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- CN112830825A CN112830825A CN201911063984.7A CN201911063984A CN112830825A CN 112830825 A CN112830825 A CN 112830825A CN 201911063984 A CN201911063984 A CN 201911063984A CN 112830825 A CN112830825 A CN 112830825A
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- biofertilizer
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention provides a bean dreg biofertilizer technology, which comprises the following steps: s1, taking bean dregs for sterilization and then carrying out drying treatment; s2, inoculating Aspergillus niger and Bacillus subtilis on a culture medium for culture, washing mature spores, filtering, and stirring to prepare a spore suspension; s3, mixing the bean dregs with the spore suspension, fermenting, culturing, sterilizing and drying to obtain fermented bean dregs; s4, mixing the pepper, the copper hammerhead ribbon, the ovateleaf holly bark, the curculigo orchioides and the cassia seeds, powdering, soaking with water, inoculating lactobacillus, adjusting the pH value, fermenting, culturing, sterilizing, and continuously drying to obtain fermented traditional Chinese medicine; s5, mixing the fermented bean dregs and the fermented traditional Chinese medicines, adding the chicken manure, the straws and the leaves, uniformly mixing, and crushing to obtain the feed. The bean dreg biofertilizer prepared by the invention takes fermented bean dregs and fermented traditional Chinese medicines as main raw materials, has stable performance, wide functions and no toxic or side effect, and has good application prospect in the aspect of crop planting.
Description
Technical Field
The invention relates to the technical field of fertilizers, in particular to a bean dreg biofertilizer technology.
Background
Since the middle of the 60 s of the 20 th century, the structure of the fertilizer in China is fundamentally changed, and the proportion of the organic fertilizer in the total fertilizer consumption is reduced from 100% in 1949 to about 50% at present. The chemical fertilizer is applied in large quantity, and plays a great role in increasing the yield in agricultural history in China. But the use of chemical fertilizers only for decades has revealed its drawbacks: the method has the advantages of great damage to the cultivated land quality, soil pollution, soil consolidation, soil fertility reduction and agricultural product quality reduction. The development of ecological agriculture emphasizes the inapplicable or less applicable chemical synthetics. Natural resources are fully utilized through biological measures, and development of biological fertilizers must be vigorously developed.
The biological fertilizer is a novel bacterial fertilizer which is efficient, nontoxic, pollution-free and nuisanceless, and can meet the requirements of people on keeping ecological balance, reasonable development and nature reformation. A great deal of biological fertilizer development is available, including biological fertilizer of single bacterial species and biological fertilizer of compound bacterial species, but has certain defects in the aspects of bacterial species efficacy and rich nutrition supply.
The soybeans are also called soybeans, green black beans and mud beans, are various in variety, including winter beans, autumn beans and kidney beans, have spherical shapes, oval shapes and the like, and have various colors, such as: greenish, black, yellow, etc. Soybeans are used in a wide variety of applications and can be processed into various bean products or processed into soybean oil and the like. The soybean is a crop with seeds rich in vegetable protein and sugar, the nutritive value of the soybean is very high, and the soybean is mainly produced in northeast China. In the industrial production of soybean, a lot of bean dregs are produced, which account for 13% -18% of the dry weight of the whole bean, the dry matter contains 55% -65% of carbohydrate and 21% -26% of protein, and further contains various minerals and trace elements, and can be used for extracting soluble dietary fiber or preparing insoluble fiber, and some oligopeptides also have special physiological functions. The biological fertilizer prepared from the microbial organic fertilizer has rich nutrition and can provide sufficient raw materials for microorganisms.
Disclosure of Invention
The invention provides a bean dreg biofertilizer technology, which can be used for obtaining various traditional Chinese medicine active ingredients and fermentation products, promoting the absorption of nutrient components in soil and improving the crop yield.
The invention provides a bean dreg biofertilizer technology which comprises the following steps:
s1, sterilizing bean dregs, putting the bean dregs into a drying box for drying treatment, drying at the temperature of 55-65 ℃ until the weight is not changed, and cooling to room temperature;
s2, inoculating Aspergillus niger and Bacillus subtilis on an agar slant culture medium, culturing at 28 ℃ for 24-48h, adding sterile physiological saline, shaking to wash down mature spores, filtering with sterile gauze, transferring into a triangular flask, and stirring on a magnetic stirrer for 0.5-1h to disperse the spores to prepare spore suspension;
s3, mixing the bean dregs obtained in the step S1 with the spore suspension obtained in the step S2, fermenting and culturing the mixture in a biochemical incubator for 48 to 72 hours, sterilizing the mixture, cooling the mixture to room temperature, continuously drying the mixture at the temperature of between 55 and 65 ℃ until the weight of the mixture is not changed, and cooling the mixture to the room temperature to obtain fermented bean dregs;
s4, mixing the pepper, the coptidis rhizoma, the ovate leaf holly bark, the common curculigo rhizome and the cassia seed, powdering, soaking with water, inoculating lactic acid bacteria, spraying HCl solution to adjust the pH, performing fermentation culture in a biochemical incubator for 24-72h, sterilizing, cooling to room temperature, continuously performing drying treatment, drying at 105 ℃ until the weight is not changed, and cooling to the room temperature to obtain the fermented traditional Chinese medicine;
s5, mixing the fermented bean dregs obtained in the step S3 with the fermented traditional Chinese medicine obtained in the step S4, adding chicken manure, straws and leaves, uniformly mixing and crushing to obtain the feed.
As a further improvement of the invention, the spore suspension has a concentration of 106-107cfu/mL。
As a further improvement of the invention, the sterilization method is sterilization by water vapor at the temperature of 120-125 ℃ for 15-30 min.
As a further improvement of the invention, in the step S2, the fermentation condition in the biochemical incubator is that the temperature is 28-30 ℃ and the humidity is 70-80%; in the step S3, the fermentation conditions in the biochemical incubator are 30-45 ℃ and 65-75% of humidity.
As a further improvement of the invention, the mass ratio of the pepper, the copper hammering leucorrhea, the ovate leaf holly bark, the curculigo rhizome, the cassia seed, the bean dregs, the chicken manure, the straw and the leaves is (5-10): (10-15): (5-15): (2-5): (5-10): (30-50): (5-10): (15-30): 10.
as a further improvement of the invention, the inoculation amount of the lactic acid bacteria is 3-5%.
As a further improvement of the invention, the concentration of the HCl solution is 1-2 mol/L.
As a further improvement of the invention, the pH is adjusted to 5.5 to 6.2.
As a further improvement of the invention, the water adding amount in the step S4 is the same as the total mass of the pepper, the cuprammonium odoratum, the jiubiying, the rhizoma curculiginis and the cassia seed.
The invention further protects the bean dreg biofertilizer obtained by the bean dreg biofertilizer technology.
The invention has the following beneficial effects: the bean dreg biofertilizer prepared by the invention takes fermented bean dregs and fermented traditional Chinese medicines as main raw materials, has stable performance, wide functions and no toxic or side effect, and has good application prospect in the aspect of crop planting. The obtained biofertilizer can promote the absorption of nutrients in soil and increase the yield of crops because Aspergillus niger and Bacillus subtilis are used to ferment bean dregs to obtain a large amount of micromolecule nutrients, and lactobacillus is used to ferment traditional Chinese medicines to obtain various traditional Chinese medicine active ingredients and leavening.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely below, and it should be apparent that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A bean dreg biofertilizer technology comprises the following steps:
s1, sterilizing 30g of bean dregs (the sterilization method is sterilizing for 15min by using water vapor at 120 ℃), putting the bean dregs into a drying box for drying treatment, drying at 55 ℃ until the weight is not changed, and cooling to room temperature;
s2, inoculating Aspergillus niger and Bacillus subtilis on an agar slant culture medium, culturing at 28 ℃ for 24h, adding sterile normal saline, shaking to wash mature spores, filtering with sterile gauze, transferring into a triangular flask, stirring for 0.5h on a magnetic stirrer to disperse the spores, and making into spore suspension with spore suspension concentration of 3.22 × 106cfu/mL；
S3, mixing the bean dregs obtained in the step S1 with the spore suspension obtained in the step S2, fermenting and culturing for 48 hours in a biochemical incubator (the temperature is 28 ℃ and the humidity is 70%), sterilizing by using water vapor at 120 ℃ for 15 minutes, cooling to room temperature, continuing drying, drying at 55 ℃ until the weight is not changed, and cooling to the room temperature to obtain fermented bean dregs;
s4, mixing 5g of pepper, 10g of copper hammerhead, 5g of ovateleaf holly bark, 2g of rhizoma curculiginis and 5g of semen cassiae, powdering, adding 27g of water for wetting, inoculating lactic acid bacteria, wherein the inoculation amount is 3%, spraying HCl solution (the concentration of the HCl solution is 1mol/L) to adjust the pH value to 5.5, fermenting and culturing for 24 hours in a biochemical incubator (the temperature is 30 ℃ and the humidity is 65%), sterilizing by using 120 ℃ water vapor for 15min, cooling to the room temperature, continuing drying, drying at the temperature of 105 ℃ until the weight is not changed, and cooling to the room temperature to obtain the fermented traditional Chinese medicine;
s5, mixing the fermented bean dregs obtained in the step S3 with the fermented traditional Chinese medicine obtained in the step S4, adding 5g of chicken manure, 15g of straws and 10g of leaves, uniformly mixing, and crushing to obtain the traditional Chinese medicine.
Example 2
A bean dreg biofertilizer technology comprises the following steps:
s1, sterilizing 50g of bean dregs (the sterilization method is to sterilize the bean dregs by using steam at 125 ℃ for 30min), putting the bean dregs into a drying box for drying treatment, drying the bean dregs at 65 ℃ until the weight of the bean dregs is not changed, and cooling the bean dregs to room temperature;
s2, inoculating Aspergillus niger and Bacillus subtilis on an agar slant culture medium, culturing at 28 ℃ for 48h, adding sterile normal saline, shaking to wash mature spores, filtering with sterile gauze, transferring into a triangular flask, stirring for 1h on a magnetic stirrer to disperse the spores, and making into spore suspension with spore suspension concentration of 7.35 × 106cfu/mL；
S3, mixing the bean dregs obtained in the step S1 with the spore suspension obtained in the step S2, performing fermentation culture in a biochemical incubator (with the temperature of 30 ℃ and the humidity of 80%) for 72 hours, and then sterilizing, wherein the sterilization method comprises the steps of sterilizing by using water vapor with the temperature of 120 ℃ and 125 ℃ for 30 minutes, cooling to room temperature, continuing drying, drying at the temperature of 65 ℃ until the weight is not changed, and cooling to the room temperature to obtain fermented bean dregs;
s4, mixing 10g of pepper, 15g of copper hammerhead, 15g of ovateleaf holly bark, 5g of rhizoma curculiginis and 10g of semen cassiae, powdering, adding 55g of water for wetting, inoculating lactic acid bacteria, wherein the inoculation amount is 5%, spraying HCl solution (the concentration of the HCl solution is 2mol/L) to adjust the pH value to 6.2, fermenting and culturing for 72 hours in a biochemical incubator (the temperature is 45 ℃ and the humidity is 75%), sterilizing by using 125 ℃ steam for 30min, cooling to the room temperature, continuing drying, drying at the temperature of 105 ℃ until the weight is not changed, and cooling to the room temperature to obtain the fermented traditional Chinese medicine;
s5, mixing the fermented bean dregs obtained in the step S3 with the fermented traditional Chinese medicine obtained in the step S4, adding 10g of chicken manure, 30g of straws and 10g of leaves, uniformly mixing, and crushing to obtain the traditional Chinese medicine.
Example 3
A bean dreg biofertilizer technology comprises the following steps:
s1, taking 40g of bean dregs, sterilizing (the sterilization method is that water vapor at 122 ℃ is used for sterilizing for 22min), then putting the bean dregs into a drying box for drying treatment, drying at the temperature of 60 ℃ until the weight is not changed, and cooling to room temperature;
s2, inoculating Aspergillus niger and Bacillus subtilis on an agar slant culture medium, culturing at 28 ℃ for 36h, adding sterile normal saline, shaking to wash mature spores, filtering with sterile gauze, transferring into a triangular flask, stirring for 0.75h on a magnetic stirrer to disperse the spores, and making into spore suspension with spore suspension concentration of 6.52 × 106 cfu/mL；
S3, mixing the bean dregs obtained in the step S1 with the spore suspension obtained in the step S2, fermenting and culturing for 60 hours in a biochemical incubator (the temperature is 29 ℃ and the humidity is 75%), sterilizing by using 122 ℃ steam for 22 minutes, cooling to room temperature, continuing drying, drying at the temperature of 60 ℃ until the weight is not changed, and cooling to the room temperature to obtain fermented bean dregs;
s4, mixing 8g of pepper, 12g of copper hammerhead, 10g of ovateleaf holly bark, 3g of rhizoma curculiginis and 7g of semen cassiae, powdering, adding 40g of water for wetting, inoculating lactic acid bacteria, wherein the inoculation amount is 3-5%, spraying HCl solution (the concentration of the HCl solution is 1.5mol/L) for adjusting the pH value to 6, performing fermentation culture in a biochemical incubator (the temperature is 36 ℃ and the humidity is 70%) for 48 hours, sterilizing by using 122 ℃ steam for sterilization for 17min, cooling to the room temperature, continuing drying, drying at the temperature of 105 ℃ until the weight is not changed, and cooling to the room temperature to obtain the fermented traditional Chinese medicine;
s5, mixing the fermented bean dregs obtained in the step S3 with the fermented traditional Chinese medicine obtained in the step S4, adding 7g of chicken manure, 22g of straws and 10g of leaves, uniformly mixing, and crushing to obtain the traditional Chinese medicine.
Test example 1
Cucumber cultivation was carried out using the bean dreg biofertilizers prepared in examples 1-3 and a commercially available bean dreg biofertilizer (purchased from Guanyue treasure shop, the development area of Danyang), the fertilization method and daily maintenance management were carried out according to a conventional method, and finally the per mu yields and average individual fruit weights of each group of cucumbers were compared, and the results are shown in Table 1.
TABLE 1
| Mu yield (kg/mu) | Average weight of Single fruit (g) | |
| Example 1 | 8753* | 352* |
| Example 2 | 8944* | 384* |
| Example 3 | 9025* | 375* |
| Is commercially available | 6673 | 202 |
Note: p <0.05 compared to the commercial group
Compared with the prior art, the bean dreg biofertilizer disclosed by the invention takes fermented bean dregs and fermented traditional Chinese medicines as main raw materials, is stable in performance, wide in function, free of toxic and side effects, and good in application prospect in the aspect of crop planting. The obtained biofertilizer can promote the absorption of nutrients in soil and increase the yield of crops because Aspergillus niger and Bacillus subtilis are used to ferment bean dregs to obtain large-amount small-molecular nutrients and lactic acid bacteria are used to ferment Chinese medicines to obtain various Chinese medicine active components and leavening.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. The bean dreg biofertilizer technology is characterized by comprising the following steps:
s1, sterilizing bean dregs, putting the bean dregs into a drying box for drying treatment, drying at the temperature of 55-65 ℃ until the weight is not changed, and cooling to room temperature;
s2, inoculating Aspergillus niger and Bacillus subtilis on an agar slant culture medium, culturing at 28 ℃ for 24-48h, adding sterile physiological saline, shaking to wash down mature spores, filtering with sterile gauze, transferring into a triangular flask, and stirring on a magnetic stirrer for 0.5-1h to disperse the spores to prepare spore suspension;
s3, mixing the bean dregs obtained in the step S1 with the spore suspension obtained in the step S2, placing the mixture in a biochemical incubator for fermentation culture for 48-72 hours, sterilizing, cooling to room temperature, continuing drying, drying at the temperature of 55-65 ℃ until the weight is not changed, and cooling to room temperature to obtain fermented bean dregs;
s4, mixing the pepper, the coptidis rhizoma, the ovate leaf holly bark, the common curculigo rhizome and the cassia seed, powdering, soaking with water, inoculating lactic acid bacteria, spraying HCl solution to adjust pH, fermenting and culturing in a biochemical incubator for 24-72h, sterilizing, cooling to room temperature, continuously drying, drying at 105 ℃ until the weight is not changed, and cooling to the room temperature to obtain the fermented traditional Chinese medicine;
s5, mixing the fermented bean dregs obtained in the step S3 with the fermented traditional Chinese medicine obtained in the step S4, adding chicken manure, straws and leaves, uniformly mixing and crushing to obtain the feed.
2. The bean dreg biofertilizer technology as claimed in claim 1, wherein the concentration of the spore suspension is 106-107cfu/mL。
3. The bean dreg biofertilizer technology as claimed in claim 1, wherein the sterilization method is sterilization with steam at 125 ℃ for 15-30 min.
4. The bean dreg biofertilizer technology according to claim 1, wherein the fermentation conditions in the biochemical incubator in step S2 are 28-30 ℃ and 70-80% humidity; in the step S3, the fermentation conditions in the biochemical incubator are 30-45 ℃ and 65-75% of humidity.
5. The bean dreg biofertilizer technology as claimed in claim 1, wherein the mass ratio of the pepper, the coptidis rhizoma, the ovate leaf holly bark, the curculigo orchioides, the cassia seeds, the bean dregs, the chicken manure, the straw and the leaves is (5-10): (10-15): (5-15): (2-5): (5-10): (30-50): (5-10): (15-30): 10.
6. the bean dreg biofertilizer technology according to claim 1, wherein the inoculation amount of lactic acid bacteria is 3-5%.
7. The bean dreg biofertilizer technology as claimed in claim 1, wherein the concentration of the HCl solution is 1-2 mol/L.
8. The okara biofertilizer technology according to claim 1, wherein the pH is adjusted to 5.5-6.2.
9. The bean dreg biofertilizer technology as claimed in claim 1, wherein the water addition amount in step S4 is the same as the total mass of pricklyash peel, cuprammonium odoratum, holly bark, curculigo orchioides and cassia seed.
10. An okara biofertilizer obtained by an okara biofertilizer technique according to any one of claims 1 to 9.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113307706A (en) * | 2021-06-30 | 2021-08-27 | 上海为绿景观建设有限公司 | Composting method of agricultural solid waste, fertilizer product and application of fertilizer product |
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| CN108440037A (en) * | 2018-06-25 | 2018-08-24 | 毕言铎 | A kind of growth promoting increases income organic fertilizer and preparation method thereof |
| US20180327330A1 (en) * | 2017-05-11 | 2018-11-15 | Xiong Bing Hong | Organic Fertilizer for Vegetables and Its Preparation Method |
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| CN103274834A (en) * | 2013-06-09 | 2013-09-04 | 苏州仁成生物科技有限公司 | Special insecticidal sterilizing microbial fertilizer for garlic and preparation method thereof |
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Application publication date: 20210525 |