CN103098980A - Microorganism straw feed fermenting agent - Google Patents

Microorganism straw feed fermenting agent Download PDF

Info

Publication number
CN103098980A
CN103098980A CN2013100326923A CN201310032692A CN103098980A CN 103098980 A CN103098980 A CN 103098980A CN 2013100326923 A CN2013100326923 A CN 2013100326923A CN 201310032692 A CN201310032692 A CN 201310032692A CN 103098980 A CN103098980 A CN 103098980A
Authority
CN
China
Prior art keywords
fermentation
straw feed
fermenting agent
straw
saccharomycete
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2013100326923A
Other languages
Chinese (zh)
Inventor
辛亚平
昝林森
杜双田
田万强
刘晓辉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Northwest A&F University
Original Assignee
Northwest A&F University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Northwest A&F University filed Critical Northwest A&F University
Priority to CN2013100326923A priority Critical patent/CN103098980A/en
Publication of CN103098980A publication Critical patent/CN103098980A/en
Pending legal-status Critical Current

Links

Landscapes

  • Fodder In General (AREA)

Abstract

The invention discloses a novel microorganism straw feed fermenting agent. The microorganism straw feed fermenting agent proportionally consists of lactobacillus, bacillus subtilis, saccharomycetes and aspergillus niger. The novel microorganism straw feed fermenting agent has the advantages of high vitality, short fermenting time and growth promotion and disease prevention effects, and strains comply with relevant stipulations of the state; a product is high in stability, is good in liquidity, is capable of being uniformly mixed with raw materials of a straw feed and is convenient in use; and a thallus is pure, low in infectious microbe content and high in safety, so that the novel microorganism straw feed fermenting agent is a green feed fermenting agent free of toxin, residue and drug resistance generation.

Description

The agent of a kind of microbial straw feed fermentation
Technical field
The present invention relates to a kind of leavening, particularly a kind of microbial straw feed fermentation agent.
Background technology
China founds a state with agriculture, is grain-production big country, is also stalk big producing country; Roughly with 1 mu of corn and wheatland energy 800 kilograms of maize straws of output and 400 kilograms of wheat stalks estimations respectively, approximately more than 7.0 hundred million ton of the stalk output in China every year, wherein the cereal crops stalk has accounted for 90.5% of total amount, maize straw accounts for 36.7%, rice straw accounts for 27.5%, and wheat stalk accounts for 26.3%.The feeding value of utilizing microbial fermentation technology to improve stalk has become the focus of herding circle, the research of feed circle.The biological straw fermented feed is a kind of nuisanceless, noresidue, pollution-free, the biological high-tech novel fodder of clean and environment-friendly, to utilize the reasonable constituents such as basidiomycetes, Aspergillus, Trichoderma, lactic acid bacteria, saccharomycete, bacillus, photosynthetic bacteria and monad, mutually collaborative, symbiosis is good, and comprehensive function is strong.Screening, the optimum organization of fermentating formula, the optimization of fermentation culture conditions, excellent species seed selection by microorganism fungus kind, set up practicable process conditions, the development of new additive for microbe feedstuff, improve the straw feed nutritive value, be to solve the protein feeds shortage, improve the emphasis of straw feed utilization rate.
Probiotic claims again regulator or bacteria-promoting agent, refers to have the microorganism formulation that prevents from suffering from diarrhoea and promote the growth of animal effect.Because probiotic does not have antibiotic residual, drug resistance problem, animal is not had any harmful effect, application prospect is more wide.Particularly to promoting growth, the general level of the health that improves animal has good action.Probiotic is as a class feed addictive, and the position of working is the digestive organs such as intestines and stomach, and main function is protected GI intrinsic profitable strain for regulating animal micro ecology of gastrointestinal tract balance, destroys GI pernicious bacteria.The action principle of probiotic, the one, set up enteron aisle effective microbe dominant population, suppress the harmful bacteria population; The 2nd, the probiotic miospore consumes the oxygen in enteron aisle in a large number when enteron aisle is sprouted, cause the enteron aisle anaerobic environment, has improved micro-ecological environment; The 3rd, be adsorbed onto competitively on enteron aisle with harmful bacteria; The 4th, produce lactic acid, acetic acid, propionic acid etc. at enteron aisle the jejunal content pH value is descended, keep micropopulation in normal enteron aisle, can prevent or treat diarrhea; The 5th, reduce the generation of harmful substance, reduce ammonia amount in the interior ight soil of intestines and blood; The 6th, produce hydrogen peroxide, suppress and the kill harmful bacterial micro-organism; The 7th, metabolism produces multiple digestive ferment and the B family vitamins such as amylolytic enzyme, protease in enteron aisle, increases absorbing of the mineral matter elements such as calcium in blood, magnesium.
And there is following shortcoming in probio use procedure of the prior art:
1) probio is generally directly added to without In Vitro Fermentation and feeds in concentrated feed to animal, has affected feeding effect.
2) result of use of probiotic is unstable, due to the interference of the original pernicious bacteria of enteron aisle, make probiotic can not be in enteron aisle field planting and playing a role well.First do pre-treatment with antibiotic, can play the effect of clearing up in advance enteron aisle.If but continue the long-term antibiotic that uses, and not only easily cause drawbacks such as producing drug resistance and medicament residue, also be easy to destroy the intestinal microecology balance, be unfavorable for the prebiotic effect of the normal profitable strain of enteron aisle.
3) treatment of present Animal diseases is still mainly take antibiotic as main, and probiotic is auxiliary.The treatment speed of probiotic is rapid not as antibiotic, and preventive effect is greater than therapeutic action.
4) in probiotic, microbe species is too many, exists the mutual restrictive function of relative complex between microorganism, as antagonism, alternate, neutrality, dwell give birth to, help give birth to, wilfully, parasitic and the effect such as engulf.Weakened on the contrary the effect of probiotic, therefore good probiotic is not to weigh with few with the many of microbe species, but decides with action effect.
5) aspect the probiotic bacteria containing amount, be not that to contain the bacterium number The more the better, should pay attention to addition and result of use.Bacteria containing amount is too high, has on the one hand increased the material consumption of microorganism in intestines, causes food consumption to increase and output does not increase; Too much microorganism excretes with ight soil on the other hand, causes waste.
6) Lactobacillus in probiotic is more unstable, can not tolerate the destruction of HTHP in the feed process, can not long-term storage.
Summary of the invention
The object of the invention is to, the agent of a kind of microbial straw feed fermentation is provided, adopt this microbial straw feed fermentation agent, have that cost is low, digestibility is high, good palatability, making length in season, long shelf-life, farming season out of question, the characteristics such as nontoxic, simple for production, that protein content is high, nutritional labeling is abundant.
In order to realize above-mentioned task, the present invention takes following technical solution:
The agent of a kind of microbial straw feed fermentation is characterized in that, forms in mass ratio maize straw leavening, wheat stalk leavening and rice straw leavening by Bacillus acidi lactici, bacillus subtilis, saccharomycete and black-koji mould, wherein;
The mass ratio of maize straw leavening is:
Bacillus acidi lactici: bacillus subtilis: saccharomycete: black-koji mould=1.0:2.5:5.0:1.0;
The mass ratio of wheat stalk leavening is:
Bacillus acidi lactici: bacillus subtilis: saccharomycete: black-koji mould=1.0:2.8:5.0:1.0;
The mass ratio of rice straw leavening is:
Bacillus acidi lactici: bacillus subtilis: saccharomycete: black-koji mould=1.0:3.0:5.0:1.0
Utilize this microbial straw feed fermentation agent production biological straw fermented feed, according to modern germ theory, using gene engineering, molecular engineering, Fermentation Engineering, enzyme engineering, Animal nutrition balance theory, adopt solid deep layer static fermentation, by the heat power dry technology, again by the animal nutritional need, production biological straw fermented feed, to the development of straw animal and comprehensive utilization of crop straw resources highly significant.Cellulase can improve degraded and the conversion of fibrous matter in feedstuff, thereby is absorbed by animal, plays an important role to improving food conversion ratio; The biological straw fermented feed is a kind of nuisanceless, noresidue, pollution-free, the biological high-tech novel fodder of clean and environment-friendly; Be the novel fodder new varieties of encourage growth in national feed product, vast potential for future development is arranged.
The specific embodiment
The present embodiment provides the agent of a kind of microbial straw feed fermentation, forms maize straw leavening, wheat stalk leavening and rice straw leavening by Bacillus acidi lactici, bacillus subtilis, saccharomycete, black-koji mould according to mass ratio, wherein;
The mass ratio of maize straw leavening is:
Bacillus acidi lactici (A): bacillus subtilis (B): saccharomycete (C): black-koji mould (D)=1.0:2.5:5.0:1.0;
The mass ratio of wheat stalk leavening is:
Bacillus acidi lactici (A): bacillus subtilis (B): saccharomycete (C): black-koji mould (D)=1.0:2.8:5.0:1.0;
The mass ratio of rice straw leavening is:
Bacillus acidi lactici (A): bacillus subtilis (B): saccharomycete (C): black-koji mould (D)=1.0:3.0:5.0:1.0.
Bacillus acidi lactici can be lactic acid with breakdown of glucose, produces ethanol, acetic acid, carbon dioxide and hydrogen etc., and is acidproof, and generally under anaerobic breeding can suppress the various bacteria growth, improves the output of immunoglobulin A.
Bacillus subtilis is aerobic bacteria, can consume rapidly the oxygen free gas in environment in alimentary canal, form the enteron aisle low-oxygen environment, promote useful anaerobic bacteria Growth and reproduction, produce the organic acids such as lactic acid, reduce the enteron aisle pH value, improve the growing environment of Bacillus acidi lactici, keep the intestinal microecology system surely to weigh, improve the animal body resistance against diseases, reduce the enterogastric diseases occurrence probability, the conditioned pathogen of pathogenic bacteria or autogenous infection is had obvious inhibitory action, indirectly suppress other pathogenic bacteria growth.
Saccharomycete can the manufacture order cell protein, B family vitamin, nucleic acid and mineral matter, also can produce some health care active materials simultaneously.
Black-koji mould destroys the plant cell wall in feed, the macromolecular substances such as cellulose, pectic substance is degraded to monose and oligosaccharides, and can produces multiple digestive ferment, amino acid, vitamin and mycoprotein; Promote growth of animal, improve immunity of organism, suppress and kill harmful microorganism.
The selection of bacterial classification is vital in straw fermented feed, and bacterial classification commonly used has mould, saccharomycete, lactic acid bacteria, actinomyces etc. at present.Be not that bacterial classification is better during fermented stalk, the factors such as the adding proportion of bacterial strain and order, condition of culture can affect the straw fermented feed effect.Mixed culture mould and yeast improve the stalk protein content greatly, and protein content increases with the increase of yeast specie, and saccharomycete not only can play derepression, and the growth of fungi is had positive-effect.
Below provide the concrete specification requirement of microbial straw feed fermentation agent:
1, the requirement of bacterial classification:
The energy decomposition of cellulose can utilize inorganic nitrogen to be converted into mycoprotein, can synthesize and secrete nutriment, palatability that can feed change can produce multiple catabolic enzyme, does not produce noxious material, have somatotrophic characteristics, thalline patience is high, is not easy self-dissolving and decomposes.
2, the composition of bacterial classification:
Bacterial classification comprises Bacillus acidi lactici, bacillus subtilis, saccharomycete, aspergillus niger.Wherein, lactic acid bacteria changes palatability, and bacillus subtilis reduces enteron aisle pH value, and saccharomycete improves protein content, and black-koji mould decomposes fiber.
3, determining of optimum growth temperature:
Take pH as 7 design temperature gradients, be a gradient from 2 ℃ of 22 ℃~32 ℃ every increases, determine by the thalli growth situation optimum temperature range that degraded cellulose is required.
4, culture medium:
Culture medium is holocellulos powder-MandelsShi nutritive salt agar double-layer plating medium, its formula is: potassium dihydrogen phosphate 2.0g, sulfate of ammoniac 1.4g, epsom salt 0.3g, calcium chloride 0.3g, green-vitriol 0.005g, manganese sulfate 0.0015g, zinc chloride 0.0017g, cobalt chloride 0.002g, sucrose 25g, water 1000ml, pH=7.0.
5, operating procedure:
Comprise the steps such as the preparation, sterilization of culture medium, cooling, inoculation, mark, inversion cultivation, observation or detection.Wherein:
The preparation of A, culture medium: holocellulos powder-MandelsShi nutritive salt agar double-layer plating medium method routinely is prepared.
B, sterilization: utilize high-pressure steam sterilizing pan to sterilize.
C, cooling: be cooled to fermentation temperature from sterilising temp.
D, inoculation: plate streak.
E, mark: every bottle of culture medium is all labelled, and indicates preparation sterilization and title, date or shelf-life.
F, inversion are cultivated: according to the characteristic of thalline, in different temperatures, be inverted culture dish under illumination and cultivate thalline.Be inverted to cultivate and to reduce scattering and disappearing of moisture in culture dish, to guarantee strain growth.
The difference of G, observation or detection microscopic examination colony colour and shape and different strains.
6, the anti-test of different strain association
1) test material
Select Bacillus acidi lactici, bacillus subtilis, saccharomycete and 4 kinds of bacterial strains of black-koji mould.
2) method of operating
Comprise the preparation of culture medium, sterilization, cooling, inoculation, mark is inverted and is cultivated, the steps such as observation or detection, wherein:
The preparation of A, culture medium:
Preparation PDA culture medium (potato agar culture medium): take required peeling potato (potato that sprouts can not be used), be cut into small pieces, add water boil 20min or soak 1h in the hot water of 80 ℃, with 4~6 layers of filtered through gauze, be configured to 20% potato and soak juice; Every 100ml potato is soaked juice and adds glucose 2g, and heating adds 2g agar after boiling again, continues heating and dissolves and supply dehydration; 121 ℃, 30min sterilization, packing, packing namely gets solid-state PDA culture medium.
B, inoculation:
Adopt the dibbling method.Each is processed and does 3 repetitions.Attention is made sure to keep in mind and transfer needle calcination on alcolhol burner flame will be sterilized when changing bacterial classification, and when it recovered preference temperature, the lower a kind of bacterial classification of inoculation was on culture medium.
C, inversion are cultivated:
Cultivated 3~5 days under 28 ℃ of constant temperatures, observe and record.Select several groups of many strain combinations (table 1) that antagonism does not occur, do the solid fermentation test.
Table 1: the antagonistic experiment combination of bacterial classification
Figure BDA00002784259000071
Annotate: "+" shows and adds bacterial classification on culture medium.A, B, C, D are the bacterial classification (A represents Bacillus acidi lactici, and B represents bacillus subtilis, and C represents saccharomycete, and D represents black-koji mould) that filters out.
7, solid fermentation experiment
1) preparation of seed bacterial classification
Choose bacterial classification: Bacillus acidi lactici (A), bacillus subtilis (B), saccharomycete (C) and 4 kinds of bacterial classifications of black-koji mould (D).
First enlarge respectively with bran mass and cultivate the bacterial classification of selecting.Wheat bran is cultivated the preparation method: the wheat bran of weighing 25g, add the water of total amount 60%, and mixing makes wheat bran fully absorb moisture; Be in the triangular flask of 150ml with the capacity of packing into of the wheat bran after mixing; Seal with sealed membrane; Mark, sterilization, cooling rear bottling (water content is 60% for 150ml, wheat bran 25g/ bottle wherein).
Difference inoculating lactobacillus A, bacillus subtilis B, saccharomycete C and black-koji mould D, be placed under 28 ℃ of constant temperature and cultivated 7 days in bottled bran mass.
2) stalk fermentation formula
The stalk fermentation formula is made (table 2) according to the following table formula, carries out stalk fermentation test (water yield that adds designs by following requirement of experiment).Note material soluble in water first water-soluble (as sugarcane during preparation
Sugar, urea and salt), then pour in the material of mixing and mix.
Table 2: stalk fermentation formula (﹪)
Figure BDA00002784259000081
3) experimental condition optimization
Screen best fermentation condition by orthogonal test, 3 factor 3 horizontal quadrature test L9(3 are selected in the fermented by mixed bacterium test 3) the confirmed test scheme, namely divide 3 horizontal research experiment (table 3) with fermentation medium composition, bacterium liquid inoculative proportion and material-water ratio, cultivate under 28 ℃ of constant temperature, measure its crude protein and crude fiber content, and thick protein increment rate and coarse-fibred degradation rate.The thermometer that to sterilize by the sealed membrane of triangular flask inserts culture medium, every the 24h record once, measures bacterial classification variations in temperature in growth course, when its temperature descends through the steady of acme, stops fermentation (table 4).
Table 3: orthogonal test factor level table
Figure BDA00002784259000082
In table: A, B, C, D are the bacterial classification (A represents Bacillus acidi lactici, and B represents bacillus subtilis, and C represents saccharomycete, and D represents black-koji mould) that filters out.
Table 4: the optimum organization of fermentation medium, mixed bacteria inoculative proportion, material-water ratio
Figure 20131003269231000021
Figure BDA00002784259000091
8, bacterial classification preparation method
Bacterial classification divides solid spawn and liquid spawn.Liquid spawn be suitable for large-scale production, but one-time investment is large for adopting the bacterial classification of fermentation tank Liquid Culture, and energy consumption is high, requires auxiliary equipment and professional technical high, and bacterial classification is deposited difficulty; The Liquid Culture production of hybrid seeds time is short, and efficient is high, and spawn activity is strong, and after bacterial classification access raw material, breeding is fast.Solid spawn produces that to have method simple, invest littlely, and bacterial classification such as easily deposits at the characteristics, and shortcoming is that the production cycle is long, and efficient is low, and bacterial classification accesses raw material has the lag phase longer.
Liquid fermentation process: bacterial growth fermentation tank---recovery system---microencapsulation---stiffening system---recovery system---freeze-drying finished product.
Solid-state fermentation process is: slant strains---first class inoculum---second class inoculum---------drying---pulverizing---finished product that adds carrier of producing fermentation.
9, nutrient component determining before and after the fermentation
Nutrient component determining: comprise determination of moisture (GBT6436-92), crude protein is measured (Micro-kjoldahl method), coarse-fibred mensuration (GBT6434-2006), the mensuration of lignin (Klason method), fermentation yield rate (the front dry biomass of dry biomass/fermentation after fermentation yield rate=fermentation).
Adopt the microbial straw feed fermentation agent of the present embodiment to nutritional labeling (seeing Table 5) before and after the large powder of straw fermentation of corn, wheat and paddy rice three.(per kilogram powder of straw 25g leavening)
Table 5: nutritional labeling unit: % before and after three large powder of straw fermentations
Figure BDA00002784259000092
Figure BDA00002784259000101
10, straw fermented feed manufacturing process is controlled
For aerobic solid fermentation process, at the fermentation initial stage, often room temperature, material temperature are all lower, should improve room temperature this moment as far as possible the production bacterium is grown as early as possible, in time suppress varied bacteria growing.Answer simultaneously moisturizing, the compost surface is covered with produce bacterium, reduce microbes in air and pollute.
Straw fermented feed fabrication processing: raw material---batching---sterilization---inoculation---primary fermentation---after fermentation---drying---check---finished product (straw fermented feed).
Wherein, raw material need to refer to the raw material of fermentation process;
Batching refers to prepare according to the proportioning that table 2 provides.
The general method of steam-sterilizing that adopts of sterilization;
Primary fermentation, this stage will be cultivated microbial cell, accumulates effective enzyme and metabolite thereby make microbial cell obtain amount reproduction; And then glucoside, gossypol, collagen, cellulose etc. are decomposed or obtain more microprotein;
After fermentation refers to existing microbial cell is carried out break process;
Drying refers to the fermentation base-material dry, makes moisture meet feed request;
Check refers to fermented feed is carried out the check of the indexs such as salmonella, Escherichia coli, total number of molds, total number of bacteria, aflatoxin, isothiocyanates and crude protein, amino acid, moisture.

Claims (1)

1. microbial straw feed fermentation agent is characterized in that, forms in mass ratio maize straw leavening, wheat stalk leavening and rice straw leavening by Bacillus acidi lactici, bacillus subtilis, saccharomycete and black-koji mould, wherein;
The mass ratio of maize straw leavening is:
Bacillus acidi lactici: bacillus subtilis: saccharomycete: black-koji mould=1.0:2.5:5.0:1.0;
The mass ratio of wheat stalk leavening is:
Bacillus acidi lactici: bacillus subtilis: saccharomycete: black-koji mould=1.0:2.8:5.0:1.0;
The mass ratio of rice straw leavening is:
Bacillus acidi lactici: bacillus subtilis: saccharomycete: black-koji mould=1.0:3.0:5.0:1.0.
CN2013100326923A 2013-01-28 2013-01-28 Microorganism straw feed fermenting agent Pending CN103098980A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2013100326923A CN103098980A (en) 2013-01-28 2013-01-28 Microorganism straw feed fermenting agent

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2013100326923A CN103098980A (en) 2013-01-28 2013-01-28 Microorganism straw feed fermenting agent

Publications (1)

Publication Number Publication Date
CN103098980A true CN103098980A (en) 2013-05-15

Family

ID=48307439

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2013100326923A Pending CN103098980A (en) 2013-01-28 2013-01-28 Microorganism straw feed fermenting agent

Country Status (1)

Country Link
CN (1) CN103098980A (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103749955A (en) * 2013-12-28 2014-04-30 邵素英 Method for preparing ensilage feed by using composite microorganism fermentation agent
CN103844002A (en) * 2014-03-26 2014-06-11 金乡县大蒜研究所 Preparation method of whole garlic straw silage
CN103922824A (en) * 2014-04-18 2014-07-16 安徽省淮北市农药厂 Method for preparing foliar fertilizer with coproduct feed or organic fertilizer through wheat straws
CN104286405A (en) * 2014-10-23 2015-01-21 上海交通大学 Method for preparing feed by fermenting fresh soybean straws with compound probiotics
CN107912603A (en) * 2017-12-12 2018-04-17 湖南德人牧业科技有限公司 A kind of ramie feed and preparation method thereof
CN110522704A (en) * 2019-09-02 2019-12-03 广州美中生物科技有限公司 A kind of stalk face cleaning composition and preparation method thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1552233A (en) * 2003-05-26 2004-12-08 哈尔滨中冠生物工程有限公司 Method for producing multi-vitamins and multi-enzymes high protein microorganic agent by multi-bacteria solid fermentation
CN1698457A (en) * 2005-05-27 2005-11-23 湖南农业大学 Production method of biotic straw feedstuff
CN101058792A (en) * 2007-02-13 2007-10-24 韩农 Highly effective straw decomposition composite flora
CN102132762A (en) * 2011-03-29 2011-07-27 南开大学 Straw feed and preparation method thereof
CN102640839A (en) * 2012-04-27 2012-08-22 赤峰市农牧科学研究院 Efficient chicken manure fermenting agent and preparation method and application thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1552233A (en) * 2003-05-26 2004-12-08 哈尔滨中冠生物工程有限公司 Method for producing multi-vitamins and multi-enzymes high protein microorganic agent by multi-bacteria solid fermentation
CN1698457A (en) * 2005-05-27 2005-11-23 湖南农业大学 Production method of biotic straw feedstuff
CN101058792A (en) * 2007-02-13 2007-10-24 韩农 Highly effective straw decomposition composite flora
CN102132762A (en) * 2011-03-29 2011-07-27 南开大学 Straw feed and preparation method thereof
CN102640839A (en) * 2012-04-27 2012-08-22 赤峰市农牧科学研究院 Efficient chicken manure fermenting agent and preparation method and application thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
任佐华: "多菌种混合发酵水稻秸秆的研究", 《中国优秀硕士学位论文全文数据库-农业科技辑(月刊)》 *
孙文: "复合微生物菌剂处理玉米秸秆及对育成牛应用效果的研究", 《中国优秀硕士学位论文全文数据库-农业科技辑》 *
易安妮等: "利用多菌种固态发酵降解玉米秸秆", 《吉林农业科学》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103749955A (en) * 2013-12-28 2014-04-30 邵素英 Method for preparing ensilage feed by using composite microorganism fermentation agent
CN103844002A (en) * 2014-03-26 2014-06-11 金乡县大蒜研究所 Preparation method of whole garlic straw silage
CN103922824A (en) * 2014-04-18 2014-07-16 安徽省淮北市农药厂 Method for preparing foliar fertilizer with coproduct feed or organic fertilizer through wheat straws
CN104286405A (en) * 2014-10-23 2015-01-21 上海交通大学 Method for preparing feed by fermenting fresh soybean straws with compound probiotics
CN107912603A (en) * 2017-12-12 2018-04-17 湖南德人牧业科技有限公司 A kind of ramie feed and preparation method thereof
CN110522704A (en) * 2019-09-02 2019-12-03 广州美中生物科技有限公司 A kind of stalk face cleaning composition and preparation method thereof

Similar Documents

Publication Publication Date Title
CN102168045B (en) Bacillus subtilis preparation and preparation method thereof
CN101390566B (en) Manifold microbe mixed culture fermentation agent and method for producing high energy protein biology feedstuff
CN109251874A (en) A kind of probiotics preparation and its preparation method and application
CN101869184B (en) Microbial feed additive and preparation method thereof
CN103444985B (en) Feed additive and manufacturing method and application thereof
CN107857689A (en) The technique that a kind of bioanalysis prepares bio-organic fertilizer using chicken manure
CN106173362A (en) The method producing cattle and sheep complete feed for raw material ferment in second time with Broussonetia papyrifera branch and leaf
CN101019609A (en) Solid fermentation process for producing natto bacillus feed additive with bean dregs
CN102226162A (en) Preparation method and application of composite microbial feed additive
CN100405922C (en) Method for producing thallus protein forage by using solid state fermentation of pomace and dreg or stalks with mixed aerobic bacteria liquor
CN100408673C (en) Organic microbial composite and use
CN103988977A (en) Feed microecological preparation and preparation method thereof
CN103098980A (en) Microorganism straw feed fermenting agent
CN101849610B (en) Wrapped low-moisture rapid-orientation fermented straw nutrition forage grass
CN104012803A (en) Preparation method of fermented feed capable of preventing cow mastitis
CN106173364A (en) The method producing cattle and sheep complete feed for raw material ferment in second time with corn straw
CN102406068B (en) Method for preparing microbial fermented feed capable of keeping intestinal health of pigs
CN106234755B (en) The method for producing cattle and sheep complete feed as raw material staged fermentation using bagasse
CN1934989A (en) Microbial fodder additive
CN108485995A (en) A kind of complex micro organism fungicide and biological organic fertilizer promoting hickory chick growth
CN101584378B (en) Method for producing high protein feed by using sweet sorghum straw as raw material
CN106396862A (en) Disease controlling microbial fertilizer special for tuberous crops and preparation method thereof
CN103960463B (en) Ensiling tangerine dregs of rice feed and preparation technology thereof
CN102334602A (en) Preparation method of bioactive composite high-efficiency feed nutrient solution used for livestock and poultry
CN102823733A (en) Production method of biological feed additive based on bacillus natto

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C12 Rejection of a patent application after its publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20130515