CN102153420A - Compound biofertilizer and preparation method thereof - Google Patents
Compound biofertilizer and preparation method thereof Download PDFInfo
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- CN102153420A CN102153420A CN2010105886843A CN201010588684A CN102153420A CN 102153420 A CN102153420 A CN 102153420A CN 2010105886843 A CN2010105886843 A CN 2010105886843A CN 201010588684 A CN201010588684 A CN 201010588684A CN 102153420 A CN102153420 A CN 102153420A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to an agricultural compound biofertilizer, in particular to a compound biofertilizer capable of improving the physical and chemical properties of soil and improving the yield and quality of agricultural products and a preparation method thereof. The fertilizer contains the following materials in percentage by weight: 30-40% of organic substance, 15-25% of plant straw, 14-15% of mixed biological fermentation bacterium liquid and 30-31% of inorganic nutrient, wherein the biological bacteria used in the invention comprise Rhodopseudomonas palustris, microzyme, azotobacter, phosphorus-solubilizing Bacillus megaterium and silicate bacteria; the organic substance components are humic acid, worm excretion and bean cakes; and the inorganic nutrient comprises N, P and K nutrient. The compound biofertilizer can be applied as basal fertilizer or additional fertilizer, and is applicable to all crops. By using the compound biofertilizer, the yield increase amplitude can be 8-10%.
Description
Technical field
The present invention relates to a kind of agricultural composite bio-fertilizer, particularly a kind of physicochemical character of improving the soil, improve composite bio-fertilizer of quality of agricultural product and preparation method thereof.
Background technology
Soil is the carrier of plant-growth, can offer the most of essential element in the plant life metabolic process, but in recent years, because people blindly pursue high yield, a large amount of mineral manure is applied in the soil, caused soil harden and aging, most fertilizer is by fixed, can not be caused the wasting of resources by plant utilization.Chemical fertilizer also can produce some toxic substances in process of production simultaneously, and serious contaminate environment is so utilize a kind of novel fertilizer to come the use of all or part of replacement chemical fertilizer to be necessary.Composite biological fertilizer is a kind of beneficial microorganism new-type fertilizer that contains, this kind fertilizer not only can self is contained the nutrient supply plant absorbing utilize, also has activating soil simultaneously, discharge in the soil ability of the nutrient that can not be absorbed by crop, this kind fertilizer can also be converted into the nitrogen in the atmosphere ammonia that can be utilized by plant absorbing, can reduce the amount of application of chemical fertilizer, have good ecological, good application prospects is arranged.
Summary of the invention
The purpose of this invention is to provide a kind of physicochemical character of improving the soil, improve composite bio-fertilizer of quality of agricultural product and preparation method thereof.
For achieving the above object, the technical solution used in the present invention is:
A kind of composite bio-fertilizer: by weight percentage, the biological bacteria of the mixed fermentation of the organic matter of 30-40%, the straw of 15-25%, 14-15% and the inorganic nutrients of 30-31%.The biological bacteria of described mixed fermentation is fermented to the mixed fermentation thing of the Rhodopseudomonas palustris fermented liquid of logarithmic phase, saccharomycetes to make fermentation liquid, vinelandii fermented liquid, phosphate solubilizing bacteria fermented liquid and silicate bacteria fermented liquid, and wherein 2-4% Rhodopseudomonas palustris fermented liquid, 1-2% saccharomycetes to make fermentation liquid, 3-5% vinelandii fermented liquid, 3% phosphate solubilizing bacteria fermented liquid and 3% silicate bacteria fermented liquid mix by weight percentage.
Described straw is one or more in wheat stalk, rice straw, soybean stalk, the maize straw.Described organic matter is one or more in humic acids, wormcast, the dregs of fat, the soya-bean cake.The effective constituent of described inorganic nutrients is nitrogen, phosphorus and potassium, and by weight percentage, nitrogen, phosphorus and potassium account for 9%, 12% respectively, 10-11%.
The preparation method of composite microbiological fertilizer: by weight percentage, 15-25% drying in the sun straw is pulverized, the organic matter, 1-2% saccharomycetes to make fermentation liquid, the 2-4% Rhodopseudomonas palustris fermented liquid that then add 30-40% mix, in 30-40 ℃ of bottom fermentation 3-4 days, add 3% phosphate solubilizing bacteria, 3-5% vinelandii and 3% silicate bacteria then and continue fermentation 1-2 days in 25-30 ℃, add the inorganic nutrients of 30-31% at last, be composite bio-fertilizer.
Above-mentioned bacterial classification is bacterium commonly used, and light and bacterium are Rhodopseudomonas palustris (Rhodop seudanonaspalustris), and available from Chinese agriculture microbial strains preservation center, culture presevation number is ACCC10649; Vinelandii are azotobacter chroococcum (Azotobacter chroococcum), and available from Chinese agriculture microbial strains preservation center, culture presevation number is ACCC21686; Phosphate solubilizing bacteria be bacillus megaterium (Bacillusmegaterium de Bray) available from Chinese industrial microbial strains preservation center, culture presevation number is CICC21976; Described silicate bacteria (Silicate bacteria) is available from Chinese agriculture microbial strains preservation center, and culture presevation number is ACCC02983; Yeast is available from Chinese industrial microbial strains preservation center, and culture presevation number is ACCC20157.
The advantage that the present invention had:
1. composite bio-fertilizer of the present invention can change the physicochemical character of soil, prevents salting of soil, increases the porosity of soil, promotes the formation of soil aggregate, decomposes and is difficult to the nutrient that absorbed by crop in the soil, reduces the amount of application of chemical fertilizer.
2. composite bio-fertilizer of the present invention is equipped with straw, biological bacteria mixed fermentation because of it adds organic matter, and add inorganic nutrients again and make, its organic content height, nutritious, under action of microorganisms, fertilizer effective rate of utilization height.
3. composite bio-fertilizer of the present invention can improve crop yield, improves crop quality.
Embodiment
Below in conjunction with concrete case study on implementation composite bio-fertilizer provided by the invention and preparation method thereof is elaborated.
Embodiment 1
Composite biological fertilizer is by weight percentage: the tunning of 40% organic matter, 15% maize straw, 14% biological bacteria fermented liquid, add 31% inorganic nutrients at last, and be composite bio-fertilizer.
Preparation: 15% maize straw is dried, is ground into Powdered being placed in the fermentation vat, add an amount of water, agglomerating to hold, there is not water droplet to drip, one bumps promptly diffusing being advisable, add 40% organic matter, 1% saccharomycetes to make fermentation liquid, 2% Rhodopseudomonas palustris fermented liquid again and carry out mixed fermentation, in 30-40 ℃ of bottom fermentation 3-4 days, adding 3% bacillus megaterium, 5% azotobacter chroococcum and 3% silicate bacteria then fermented 1 day in 25-30 ℃ again, add 8% nitrogen nutrient, 12% phosphorus nutrient, 11% potassium nutrition at last, be composite bio-fertilizer.
Organic matter is humic acids and the soya-bean cake compounding substances in 1: 1 ratio.
Described Rhodopseudomonas palustris fermented liquid, saccharomycetes to make fermentation liquid, azotobacter chroococcum fermented liquid, bacillus megaterium fermented liquid and silicate bacteria fermented liquid all can make according to ordinary method, present embodiment provides is a kind of in numerous culture condition, is respectively:
The azotobacter chroococcum fermented liquid: in the liquid nutrient medium after azotobacter chroococcum sterilized, at 30-35 ℃, pH 7.2, aerobic conditions was cultivated 2-3 days down, can obtain blown-ball Azotobacter ferment liquid;
Described liquid culture based component is dipotassium hydrogen phosphate 0.08% by weight percentage, potassium primary phosphate 0.02%, sal epsom 0.02%, calcium sulfate 0.01%, iron(ic) chloride trace, Na
2MoO
42H
2The O trace, yeast extract paste 0.05%, N.F,USP MANNITOL 2%, its surplus is a distilled water;
Rhodopseudomonas palustris fermented liquid: Rhodopseudomonas palustris is inserted in the liquid nutrient medium after having sterilized under 25-30 ℃, transfer PH to 6.8-7.0, cultivated 3-4 days, can obtain the Rhodopseudomonas palustris fermented liquid;
Described liquid culture based component is, yeast powder 0.3% by weight percentage, peptone 0.3%, and sal epsom 0.05%, calcium chloride 0.03%, surplus is a distilled water.
Bacillus megaterium fermented liquid: the bacillus megaterium kind is inserted in the liquid nutrient medium after having sterilized, under 30 ℃ of conditions, transfer PH to 7.0, cultivated 1-2 days, can obtain the bacillus megaterium fermented liquid; Described liquid culture based component is, peptone 0.5%, and extractum carnis 0.3%, sodium-chlor 0.5%, its surplus is a distilled water; In every liter of substratum, add 5mg MnSO simultaneously
4H
2O.
The silicate bacteria fermented liquid: silicate bacteria is inserted in the liquid nutrient medium after having sterilized, and under 25～35 ℃, the pH value is 7.5～8.0, cultivates 2-4 days, can obtain the silicate bacteria fermented liquid; Described liquid culture based component is, sucrose 5% by weight percentage, Sodium phosphate dibasic 0.2%, and sal epsom 0.05%, lime carbonate 0.01%, iron trichloride 0.0005%, surplus is a distilled water.
Saccharomycetes to make fermentation liquid: yeast is inserted in the liquid nutrient medium after having sterilized, and pH cultivates 5～6, can obtain saccharomycetes to make fermentation liquid; Described liquid culture based component is by weight percentage: potato 20%, and glucose 2%, surplus is a distilled water.
Embodiment 2
Difference from Example 1 is:
Composite bio-fertilizer, by weight percentage, humic acids 20%, soya-bean cake 10%, mixed plant stalk 25%, 15% fermenting organism bacterium liquid i.e. the tunning of 2% yeast, 4% Rhodopseudomonas palustris, 3% phosphate solubilizing bacteria, 3% silicate bacteria, 3% vinelandii and 30% inorganic nutrients, is composite bio-fertilizer.Wherein, the mixed plant stalk is the mixture of wheat stalk, rice straw, soybean stalk and the maize straw of equivalent weight.
Preparation: by weight percentage, 25% mixed plant stalk is dried, be ground into Powdered back in fermentation vat, add an amount of water, agglomerating to hold, there is not water droplet to drip, one bumps promptly diffusing being advisable, add 20% humic acids again, 10% soya-bean cake, 2% yeast, the mixing of 4% Rhodopseudomonas palustris is fermented, in 30-40 ℃ of bottom fermentation 3-4 days, add 3% bacillus megaterium (Bacillusmegateriumde Bray) at last, 3% azotobacter chroococcum (Azotobacter chroococcum) and 3% silicate bacteria add 8% nitrogen nutrient at last in 30 ℃ of condition bottom fermentations 1 day, 12% phosphorus nutrient, 9% potassium nutrition is composite bio-fertilizer.
The present invention is applicable to all crops, applies with the base manure or the form of topdressing when using, can activating soil after using, increase soil fertility.Can obviously improve crop yield after using this product simultaneously, improve quality, output increase rate about 8～10%.
The soil organism in the fertilizer of the present invention has good effect to everyways such as soil physics, chemistry and biological chemistries.Containing elements such as very abundant phosphorus, sulphur in the organic matter, is the main existence form of plant nutrient substance in soil.Organic mineralization through microorganism discharges plant nutrient, supplies with the needs of plant and microorganism life.The soil ulmin soil ulmin that organic humification forms produces cohesion, makes the dispersive soil particle cementation become coacervate, thereby the contradiction between nutrient, moisture and the air in the adjusting soil is created the required good condition of growth and development of plants.Can improve the nutrient preserving capability of soil in addition, promote crop growth, extremely low soil ulmin molecular solution, plant there is hormesis, if can changes the metabolism of carbohydrate in the plant materials, promote the accumulation of reducing sugar, improve the osmotic pressure of cell, thereby improved the drought of doing of plant; Can improve oxidasic activity, improve the saturating property of cytolemma and, promote the growth of root system nutrient absorbing.Humic mass-energy absorption and dissolve some agricultural chemicals, and can form water-soluble complex compound with heavy metal is discharged soil with water, reduces to Toxicity in Crops with to the pollution of soil.Humic acids is the macromolecular organic matter of a class, humic acids and product-use thereof are extensive, on agricultural, combine the humic acid fertilizer made from elements such as nitrogen, phosphorus, potassium, effect all clearly, as: with in the ammonia and humic acids can be made into ammonium humate fertilizer, can improve the soil, stimulate plant growth, improve function such as agricultural product quality; Nitro humic acid can be used as the raising rice seedlings acidity regulator; Humic acids magnesium, zinc humic acid, humic acid urea iron have good effect respectively on additional soil magnesium deficiency, the scarce zinc of corn, fruit tree iron deficiency; Combined use of pesticides such as humic acids and nitrofen, atrazine can improve drug effect, suppress residual hazard; Sodium salts humic acids is fine to the effect of treatment apple tree canker.
Yeast in the biological bacteria can quicken the fermentation to stalk simultaneously; Photosynthetic bacteria can carry out physiological functions such as light compositing, aerobic repiration, fixed nitrogen, solid carbon, and rich in proteins, VITAMIN, somatomedin, nutritive ingredient such as immune factor, and have more security, photosynthetic bacteria preparation also has unique disease-resistant, promotes growth function, and it is purifying water, all is being widely used aspect the exploitation of raising fish and shrimp, its feeding, organic fertilizer and new forms of energy; And vinelandii, phosphate solubilizing bacteria, silicate bacteria can be decomposed and are difficult to be made for thing by the non-available nutrient of crop utilization in the soil and absorb.
Contain abundant nutritive element in the stalk of farm crop, according to crop material kind difference, contained nutritive element is also different, and the leguminous crop stalk is nitrogenous more, and it is many that the gramineous crop stalk contains potassium.It can improve physics, chemistry and biology proterties, the increase crop yield of soil.
Application examples
With the greenhouse tomato is subjects, the test group of design control group and use composite microbe fertilizer, and the usage quantity of composite microbe fertilizer is a 2500kg/ mu.Each test treatment group is established 5 repetitions, and each repeats 200m
2According to the ordinary method management, measure the influence of composite microbe fertilizer to tomato yield, see table 1 for details.
Table 1 is used the influence of composite microbe fertilizer to tomato yield
Claims (5)
1. composite bio-fertilizer is characterized in that: by weight percentage, and the biological bacteria of the mixed fermentation of the organic matter of 30-40%, the straw of 15-25%, 14-15% and the inorganic nutrients of 30-31%.The biological bacteria of described mixed fermentation is fermented to the mixed fermentation thing of the Rhodopseudomonas palustris fermented liquid of logarithmic phase, saccharomycetes to make fermentation liquid, vinelandii fermented liquid, phosphate solubilizing bacteria fermented liquid and silicate bacteria fermented liquid, and wherein 2-4% Rhodopseudomonas palustris fermented liquid, 1-2% saccharomycetes to make fermentation liquid, 3-5% vinelandii fermented liquid, 3% phosphate solubilizing bacteria fermented liquid and 3% silicate bacteria fermented liquid mix by weight percentage.
2. by the described composite bio-fertilizer of claim 1, it is characterized in that: described straw is one or more in wheat stalk, rice straw, soybean stalk, the maize straw.
3. by the described composite bio-fertilizer of claim 1, it is characterized in that: described organic matter is one or more in humic acids, wormcast, the dregs of fat, the soya-bean cake.
4. by claim 1 composite bio-fertilizer, it is characterized in that: the effective constituent of described inorganic nutrients is nitrogen, phosphorus and potassium, and by weight percentage, nitrogen, phosphorus and potassium account for 9%, 12% respectively, 10-11%.
5. the preparation method of the described composite microbiological fertilizer of claim 1, it is characterized in that: by weight percentage, 15-25% drying in the sun straw is pulverized, the organic matter, 1-2% saccharomycetes to make fermentation liquid, the 2-4% Rhodopseudomonas palustris fermented liquid that then add 30-40% mix, in 30-40 ℃ of bottom fermentation 3-4 days, add 3% phosphate solubilizing bacteria, 3-5% vinelandii and 3% silicate bacteria then and continue fermentation 1-2 days in 25-30 ℃, add the inorganic nutrients of 30-31% at last, be composite bio-fertilizer.
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| CN102503605A (en) * | 2011-09-29 | 2012-06-20 | 山东光大肥业科技有限公司 | Biological organic fertilizer specially used for carthamus tinctorious L. and preparation method thereof |
| CN102503606A (en) * | 2011-10-11 | 2012-06-20 | 山东光大肥业科技有限公司 | Biological fertilizer special for organic peanuts and preparation method thereof |
| CN102503630A (en) * | 2011-10-24 | 2012-06-20 | 山东光大肥业科技有限公司 | Dedicated organic/inorganic compound fertilizer for kiwi and preparation method thereof |
| CN102584487A (en) * | 2012-02-03 | 2012-07-18 | 官会林 | Special fertilizer for high-quality and high-yield green cherry culture in fresh water and sea water source areas |
| CN102875202A (en) * | 2012-10-12 | 2013-01-16 | 湖北吉农沃尔特农业有限公司 | Wormcast organic mature and preparation method thereof |
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| CN103396229A (en) * | 2013-07-31 | 2013-11-20 | 常熟市常福有机复合肥有限公司 | Selenium-enriched biological organic fertilizer |
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| CN104892210A (en) * | 2015-06-13 | 2015-09-09 | 王娟 | Plug-in type bio-fertilizer box for cherry blossom trees |
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| CN105601364A (en) * | 2016-02-18 | 2016-05-25 | 姜薇 | Preparation method of special fertilizer for corn |
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Application publication date: 20110817 |