CN113402327A - Special microbial compound fertilizer for vegetables and preparation method thereof - Google Patents
Special microbial compound fertilizer for vegetables and preparation method thereof Download PDFInfo
- Publication number
- CN113402327A CN113402327A CN202110855076.2A CN202110855076A CN113402327A CN 113402327 A CN113402327 A CN 113402327A CN 202110855076 A CN202110855076 A CN 202110855076A CN 113402327 A CN113402327 A CN 113402327A
- Authority
- CN
- China
- Prior art keywords
- vegetables
- parts
- strain
- compound fertilizer
- special
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Inorganic Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Soil Sciences (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a special microbial compound fertilizer for vegetables, which is prepared from the following components in parts by weight: 60-100 parts of a carrier and 15-25 parts of a composite liquid microbial inoculum; and provides a preparation method thereof. The special microbial compound fertilizer for vegetables and the preparation method thereof provided by the invention have the following characteristics and advantages: the rhizosphere microorganism composite inoculant can increase the yield of vegetables and improve the quality of the vegetables. In addition, the product can also be used for dissolving insoluble phosphorus in soil and fixing nitrogen in air, so that sufficient nutrients are provided for plants, and meanwhile, the absorption of the plants on other nutrient elements can be promoted, the growth of the plants can be regulated and promoted, besides, the product can also help the plants to resist the infection of pathogenic fungi, and the yield can be improved.
Description
Technical Field
The invention relates to the technical field of agricultural biology, in particular to a special microbial compound fertilizer for vegetables and a preparation method thereof.
Background
Vegetables are an important component of agricultural production, are also important sources of vitamins and mineral elements taken by people, and are daily indispensable food for human beings. Along with the improvement of living standard of people, higher requirements are also put forward on the quality of vegetables, particularly on the aspects of nutrition, sanitation and safety. However, vegetables generally have higher dependence on nitrogen and phosphate fertilizers, traditional agriculture mainly depends on chemical fertilizers to provide nutrition for the vegetables, farmers pursue higher yield, and the use amount of the chemical fertilizers seriously exceeds the standard, so that not only is the purchasing investment increased, but also the environmental problems such as soil degradation are caused, meanwhile, hidden dangers are brought to food safety, for example, leaf vegetables such as Chinese cabbages are easy to enrich nitrite, and the accumulation is aggravated by the application of excessive chemical nitrogen fertilizers, so that the harm is generated to the human health. Therefore, how to reduce the fertilizer consumption is an important issue in the current vegetable production.
In recent years, domestic and foreign researches show that a large number of growth-promoting bacteria (a large class of bacteria capable of promoting plant growth) exist in the plant rhizosphere, for example, phosphorus-dissolving bacteria can convert phosphorus which is difficult to be directly absorbed and utilized by plants in soil into a plant absorbable state; the nitrogen fixing bacteria (which are symbiotic, combined and free nitrogen fixing) can fix nitrogen in the atmosphere into a plant absorbable state; the plant hormone-secreting bacteria can secrete plant hormones (such as auxin, gibberellin, cytokinin and the like); the pathogenic antagonistic bacteria (also called biocontrol bacteria) can inhibit or kill plant pathogenic bacteria, etc. Meanwhile, some growth-promoting bacteria have the characteristics of dissolving phosphorus, fixing nitrogen, secreting phytohormone and the like. The composite inoculant (or bacterial manure) developed by using the high-efficiency growth-promoting bacteria separated from the plant rhizosphere can activate the indissolvable phosphorus source of soil, fix nitrogen in the air, provide nitrogen and phosphorus nutrition, promote the absorption of crops on other nutrient elements, improve the nutrient structure of plants, regulate and promote the growth of the plants and enhance the disease resistance of the plants. In addition, the growth-promoting bacteria can also improve the soil structure, increase the organic matter content of the soil, improve saline-alkali soil, prevent soil degradation and the like. Therefore, the research on plant growth promoting bacteria resources and the research and development of the compound inoculant (bacterial manure) have extremely important practical significance.
Nowadays, green agriculture is actively developed at home and abroad, and safe and pollution-free green food is advocated to be produced. It is desirable to produce green food products without, or with minimal (or limited) use of chemical fertilizers, pesticides, and other chemicals. It requires that the fertilizer must first protect and promote growth and improve quality of the subject to which it is applied; secondly, the generation and accumulation of harmful substances of the application object are not caused; and thirdly, no adverse effect is caused to the ecological environment.
The compound microbial fertilizer is compounded with specific phosphate solubilizing, plant hormone secreting, nitrogen fixing microbe or other identified microbe and nutrient matter, and is one kind of living microbe product capable of providing, maintaining or improving plant nutrients, raising yield of agricultural product and improving quality of agricultural product.
The compound microbial fertilizer integrates the advantages of organic fertilizer, chemical fertilizer and microbial fertilizer, overcomes the defects of large application amount and unstable quality of the organic fertilizer in the past, and is very convenient to apply. Overcomes the defects of soil physicochemical property degradation and agricultural product quality degradation caused by a single-purification chemical fertilizer.
The main characteristics are: 1. increasing soil fertility: for example, the application of nitrogen-fixing and phosphorus-dissolving microorganisms can fix nitrogen and decompose insoluble phosphorus in soil, thereby improving the effective utilization rate of chemical fertilizers, the soil fertility and the crop nutrition level. 2. Biological control of harmful microorganisms: the biocontrol bacteria grow and propagate in a large amount at the roots of the crops, play a role in antagonizing and inhibiting the growth and the propagation of pathogenic microorganisms, achieve the effect of reducing crop diseases, and improve the quality and the yield of the crops and the continuous cropping resistance.
Although more compound microbial fertilizers in the prior art are applied to agricultural production practices, most compound microbial fertilizers have far lower effect than chemical fertilizers due to unreasonable strain composition or improper preparation operation, so that the market acceptance is very low.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
The invention aims to explore, explore and utilize new microbial resources, save energy and protect environment aiming at the current situation that the chemical fertilizer is excessively depended on in vegetable planting, the use efficiency of the chemical fertilizer is reduced and the ecological environment pollution is caused in the prior art at home and abroad. The invention relates to a method for preparing a composite microbial fertilizer for vegetables, which is characterized in that a phosphorus-dissolving strain, a nitrogen-fixing strain, a plant hormone secreting strain and a pathogen antagonistic strain are screened from the rhizosphere of various vegetables in different habitats such as Gansu and Ningxia, and the like, nitrogen-fixing strain, plant hormone secreting strain and pathogen antagonistic strain are screened according to a certain proportion, so that a novel environment-friendly fertilizer and a disease-preventing composite microbial fertilizer are provided for vegetable production, an industrial fertilizer is partially replaced, the invasion of diseases at the root of the vegetables is prevented, and the purchasing investment is reduced.
The above object of the present invention can be achieved by the following technical solutions:
the invention provides a special microbial compound fertilizer for vegetables, which is prepared from the following components in parts by weight: 60-100 parts of carrier and 15-25 parts of composite liquid microbial inoculum.
Further, the composite liquid microbial agent comprises any one or more of bacillus subtilis VGP004 strain fermentation liquor, bacillus megaterium VGP625 strain fermentation liquor, azotobacter chroococcum VGP021 strain fermentation liquor and bacillus brevis VGP123 strain fermentation liquor;
can be selected as follows: the bacillus subtilis VGP004 strain fermentation liquor (004 for short) is singly used, the bacillus megaterium VGP625 strain fermentation liquor (625 for short) is singly used, the azotobacter chroococcum VGP021 strain fermentation liquor (021 for short) is singly used, the bacillus brevis VGP123 strain fermentation liquor (123 for short) is singly used, 004+625 compounding, 004+021 compounding, 004+123 compounding, 625+021 compounding, 625+123 compounding, 021+123 compounding, 004+625+021 compounding, 004+625+123 compounding, 004+021+123 compounding, 625+021+123 compounding, 004+625+021+123 compounding and the like.
The weight of the mixed strain system in the mixed strain suspension is negligible, since the proportion of the mixed strain system is less than one ten thousandth of the weight of the used culture medium.
More than 300 strains of bacteria screened from the rhizosphere of various vegetables in different habitats, such as cruciferae, cucurbitaceae, Umbelliferae, solanaceae, compositae and the like are screened, and high-efficiency phosphorus-dissolving strains, combined nitrogen-fixing strains, plant hormone-secreting strains and disease-resistant strains are screened out through the determination of various indexes such as antagonism and interaction characteristics among the strains, phosphorus dissolving, nitrogen fixation, plant hormone secretion (IAA), salt tolerance, antagonistic pathogenic bacteria and the like and the experimental determination of the growth promoting effect and the quality influence on the vegetables by indoor and field use, so that the vegetable rhizosphere composite microbial fertilizer is developed.
Further, in the microbial compound fertilizer special for vegetables, the bacillus subtilis VGP004 strain is preserved in China center for type culture Collection with the preservation number of CCTCC NO: m2021578, the preservation time is 24 days at 5 months in 2021, and the preservation address is university of Wuhan, China.
Bacillus subtilisBacillus subtilisVGP004 strain with phosphorus solubilizing, plant growth hormone secretion, Fusarium (F.) (B.) antagonistic effectFusariumspp) And Rhizoctonia solani (F.), (Rhizoctonia solani) And the like.
Further, in the microbial compound fertilizer special for vegetables, the bacillus megaterium VGP625 strain is preserved in China center for type culture Collection with the preservation number of CCTCC NO: m2021583, the preservation time is 24 days at 5 months in 2021, and the preservation address is university of Wuhan, China.
Bacillus megateriumPsdeuomnoda fluoerncne VGP625 strain with nitrogen-fixing and plant-growing secretion effectsLong hormone antagonistic fusarium (FusariumsppAnd Rhizoctonia solani: (Rhizoctonia solani) And the like.
Further, the azotobacter chroococcum VGP021 strain is preserved in the China center for type culture Collection, and the preservation number is CCTCC NO: m2021584, the preservation time is 24 days at 5 months in 2021, and the preservation address is university of Wuhan, China.
Azotobacter chroococcumAzotobacter chroococcum VGP021 strain has the functions of fixing nitrogen and secreting plant growth hormone.
Further, in the microbial compound fertilizer special for vegetables, the brevibacillus brevis VGP123 strain is preserved in China center for type culture Collection with the preservation number of CCTCC NO: m2021581, the preservation time is 24 days at 5 months in 2021, and the preservation address is university of Wuhan, China.
Brevibacillus brevisBrevibacillus brevisVGP123 strain has the functions of dissolving phosphorus, fixing nitrogen and secreting plant growth hormone.
Furthermore, in the composite liquid microbial agent, by weight, 20-40 parts of bacillus subtilis VGP004 strain fermentation liquid, 10-30 parts of bacillus megaterium VGP625 strain fermentation liquid, 10-30 parts of azotobacter chroococcum VGP021 strain fermentation liquid and 10-30 parts of brevibacillus brevis VGP123 strain fermentation liquid are contained.
Furthermore, in the special microbial compound fertilizer for vegetables, the number of thalli in the compound liquid microbial inoculum is more than or equal to 109cfu/mL; the strain culture solution is NA liquid culture medium.
The mixed strain can grow on a culture medium containing less than 5 percent of NaCl; the determination of the mixed strain system shows that no antagonistic reaction exists among the four strains, and the mixed strain system is determined in various determinations such as phosphorus dissolution, nitrogen fixation, secretion of plant growth hormone (IAA), salt tolerance and the like: the available phosphorus content is 117.5 mg.L-1Compared with the single culture of the strains in the combination, the culture efficiency is improved by 25.8-56.4%; the azotase activity is 884.2 nmol C2H4 h-1 mL-1Comparing the strains in the combination respectivelyThe yield is improved by 43.3-64.7 when the culture is carried out alone; the IAA content was 19.16. mu.g/mL-1And the content is increased by 41.3-83.4 (shown in Table 1) compared with the strains in the combination when the strains are respectively cultured independently.
Table 1 shows the comparison of the use effect of the complex microbial inoculum and the single strain microbial inoculum, which is specifically shown in the following table:
further, the carrier is prepared from the following raw materials in parts by weight: 25-35 parts of flower soil, 5-15 parts of potato residue, 20-30 parts of grass carbon and 10-20 parts of charcoal; the carrier raw materials are crushed and then screened by a 2mm sieve.
The second invention of the invention provides a preparation method of a special microbial compound fertilizer for vegetables, wherein the special microbial compound fertilizer for vegetables is the microbial compound fertilizer, and the preparation method comprises the following steps:
s1, respectively and fully drying carrier raw materials in the sun, removing impurities, crushing, sieving and fully and uniformly mixing according to a ratio; the carrier raw materials comprise, by weight, 25-35 parts of flower soil, 5-15 parts of potato residue, 20-30 parts of grass carbon and 10-20 parts of charcoal; sieving with a 2mm sieve;
s2, sterilizing the uniformly mixed carrier at high temperature and high pressure, and cooling for later use;
sterilizing at 121 deg.C under 0.1MPa for 1 min;
s3, preparing NA liquid culture medium, respectively inoculating bacterial strains and culturing, wherein the quantity of the bacterial strains is more than or equal to 109Terminating the culture when cfu/mL, and uniformly mixing the bacterial liquid to obtain a composite liquid microbial inoculum;
the culture conditions of the strains are as follows: culturing at 30 deg.C for 24-48 hr, counting thallus number in culture solution with a blood counting plate under microscope, and counting the thallus number more than 109Terminating the culture at cfu/ml;
s4, inoculating the composite liquid microbial inoculum into the sterilized carrier according to the proportion, and culturing the composite liquid microbial inoculum under an aseptic condition for use or placing the composite liquid microbial inoculum in an aseptic sealed container for normal-temperature storage for later use;
the culture under the aseptic condition comprises the following steps: culturing at 28-30 deg.C under aseptic condition for 5 days.
The special microbial compound fertilizer for vegetables and the preparation method thereof provided by the invention have the following characteristics and advantages: compared with untreated fruits and vegetables, such as tomatoes, cucumbers, peppers and the like, treated by the rhizosphere compound microbial fertilizer disclosed by the invention, the fruit yield, the single fruit weight and the variety are obviously improved, and the maturation period is averagely advanced by 3-5 days; the yield, leaf area and average plant height of leaf vegetables such as celery, Chinese cabbage and lettuce are obviously improved, and the leaf color is obviously deepened. Therefore, the rhizosphere microorganism composite inoculant can increase the yield of vegetables and improve the quality of the vegetables simultaneously. In addition, the product can also be used for dissolving insoluble phosphorus in soil and fixing nitrogen in air, so that sufficient nutrients are provided for plants, and meanwhile, the absorption of the plants on other nutrient elements can be promoted, the growth of the plants can be regulated and promoted, besides, the product can also help the plants to resist the infection of pathogenic fungi, and the yield can be improved.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
a special microbial compound fertilizer for vegetables is prepared from the following components in parts by weight: 60kg of carrier and 15kg of composite liquid microbial inoculum.
The composite liquid microbial inoculum comprises any one or more of bacillus subtilis VGP004 strain fermentation liquor, bacillus megaterium VGP625 strain fermentation liquor, azotobacter chroococcum VGP021 strain fermentation liquor and bacillus brevis VGP123 strain fermentation liquor.
In the composite liquid microbial inoculum, according to the weight ratio, the fermentation liquor of the bacillus subtilis VGP004 strain: fermentation liquor of bacillus megaterium VGP625 strain: fermentation liquor of azotobacter chroococcum VGP021 strain: the fermentation liquor of the brevibacillus brevis VGP123 strain is 2:1: 1: 1.
in the composite liquid microbial inoculum, the number of thalli is more than or equal to 109cfu/mL; the strain culture solution is NA liquid culture medium.
The carrier is prepared from the following raw materials in parts by weight: 25kg of flower soil, 5kg of potato residues, 20kg of grass carbon and 10kg of charcoal; the carrier raw materials are crushed and then screened by a 2mm sieve.
The preparation method of the special microbial compound fertilizer for the vegetables comprises the following steps:
s1, respectively and fully drying carrier raw materials in the sun, removing impurities, crushing, sieving by a 2mm sieve, and fully and uniformly mixing according to the proportion;
s2, sterilizing the uniformly mixed carrier at high temperature and high pressure, wherein the sterilization process is 121 ℃, sterilizing at high temperature and high pressure of 0.1MPa for 1min, and cooling for later use;
s3, preparing NA liquid culture media, respectively inoculating and culturing strains, wherein the strain culture conditions are as follows: culturing at 30 deg.C for 24 hr, counting thallus number in culture solution with a blood counting plate under microscope, and counting the thallus number more than 109Terminating the culture when cfu/ml, and uniformly mixing the bacterial liquid to obtain a composite liquid microbial inoculum;
s4, inoculating the composite liquid microbial inoculum into the sterilized carrier according to the proportion, and culturing the composite liquid microbial inoculum under an aseptic condition for use or placing the composite liquid microbial inoculum into an aseptic sealed container for storage at normal temperature for later use, wherein the culture under the aseptic condition is as follows: culturing at 28 deg.C for 5 days under aseptic condition.
Example 2:
a special microbial compound fertilizer for vegetables is prepared from the following components in parts by weight: 100kg of carrier and 25kg of composite liquid microbial inoculum.
The composite liquid microbial inoculum comprises any one or more of bacillus subtilis VGP004 strain fermentation liquor, bacillus megaterium VGP625 strain fermentation liquor, azotobacter chroococcum VGP021 strain fermentation liquor and bacillus brevis VGP123 strain fermentation liquor.
In the composite liquid microbial inoculum, according to the weight ratio, the fermentation liquor of the bacillus subtilis VGP004 strain: fermentation liquor of bacillus megaterium VGP625 strain: fermentation liquor of azotobacter chroococcum VGP021 strain: the fermentation liquor of the brevibacillus brevis VGP123 strain is 4:3: 3: 3.
in the composite liquid microbial inoculum, the number of thalli is more than or equal to 109cfu/mL; the strain culture solution is NA liquid culture medium.
The carrier is prepared from the following raw materials in parts by weight: 35kg of flower soil, 15kg of potato residues, 30kg of grass carbon and 20kg of charcoal; the carrier raw materials are crushed and then screened by a 2mm sieve.
The preparation method of the special microbial compound fertilizer for the vegetables comprises the following steps:
s1, respectively and fully drying carrier raw materials in the sun, removing impurities, crushing, sieving by a 2mm sieve, and fully and uniformly mixing according to the proportion;
s2, sterilizing the uniformly mixed carrier at high temperature and high pressure, wherein the sterilization process is 121 ℃, sterilizing at high temperature and high pressure of 0.1MPa for 1min, and cooling for later use;
s3, preparing NA liquid culture media, respectively inoculating and culturing strains, wherein the strain culture conditions are as follows: culturing at 30 deg.C for 48 hr, counting thallus number in culture solution with a blood counting plate under microscope, and counting the thallus number more than 109Terminating the culture when cfu/ml, and uniformly mixing the bacterial liquid to obtain a composite liquid microbial inoculum;
s4, inoculating the composite liquid microbial inoculum into the sterilized carrier according to the proportion, and culturing the composite liquid microbial inoculum under an aseptic condition for use or placing the composite liquid microbial inoculum into an aseptic sealed container for storage at normal temperature for later use, wherein the culture under the aseptic condition is as follows: culturing at 30 deg.C for 5 days under aseptic condition.
Example 3:
a special microbial compound fertilizer for vegetables is prepared from the following components in parts by weight: 80kg of carrier and 20kg of composite liquid microbial inoculum.
The composite liquid microbial inoculum comprises any one or more of bacillus subtilis VGP004 strain fermentation liquor, bacillus megaterium VGP625 strain fermentation liquor, azotobacter chroococcum VGP021 strain fermentation liquor and bacillus brevis VGP123 strain fermentation liquor.
In the composite liquid microbial inoculum, according to the weight ratio, the fermentation liquor of the bacillus subtilis VGP004 strain: fermentation liquor of bacillus megaterium VGP625 strain: fermentation liquor of azotobacter chroococcum VGP021 strain: the fermentation liquor of the brevibacillus brevis VGP123 strain is 3:2: 2: 2.
composite liquid bacterial agentIn the amount of the cells of 10 or more9cfu/mL; the strain culture solution is NA liquid culture medium.
The carrier is prepared from the following raw materials in parts by weight: 30kg of flower soil, 10kg of potato residue, 25kg of grass carbon and 15kg of charcoal; the carrier raw materials are crushed and then screened by a 2mm sieve.
The preparation method of the special microbial compound fertilizer for the vegetables comprises the following steps:
s1, respectively and fully drying carrier raw materials in the sun, removing impurities, crushing, sieving by a 2mm sieve, and fully and uniformly mixing according to the proportion;
s2, sterilizing the uniformly mixed carrier at high temperature and high pressure, wherein the sterilization process is 121 ℃, sterilizing at high temperature and high pressure of 0.1MPa for 1min, and cooling for later use;
s3, preparing NA liquid culture media, respectively inoculating and culturing strains, wherein the strain culture conditions are as follows: culturing at 30 deg.C for 36 hr, counting thallus number in culture solution with a blood counting plate under microscope, and counting the thallus number more than 109Terminating the culture when cfu/ml, and uniformly mixing the bacterial liquid to obtain a composite liquid microbial inoculum;
the preparation method of the NA liquid culture medium comprises the following steps: sequentially adding 10.0g of peptone, 3.0g of beef powder and 5.0g of sodium chloride into 1L of distilled water;
s4, inoculating the composite liquid microbial inoculum into the sterilized carrier according to the proportion, and culturing the composite liquid microbial inoculum under an aseptic condition for use or placing the composite liquid microbial inoculum into an aseptic sealed container for storage at normal temperature for later use, wherein the culture under the aseptic condition is as follows: culturing at 29 deg.C for 5 days under aseptic condition.
Example 4:
the invention provides a compound microbial fertilizer which comprises, by weight, 30 parts of flower soil, 10 parts of potato residue, 25 parts of grass peat, 15 parts of charcoal and 20 parts of liquid microbial inoculum. The liquid microbial inoculum is prepared from bacillus subtilisBacillus subtilisVGP004 strain fermentation liquor and bacillus megateriumBacillus megaterium VGP625 strain fermentation liquor and azotobacter chroococcumAzotobacter chroococcum VGP021 strain fermentation liquid and Brevibacillus brevisBrevibacillus brevis The VGP123 strain fermentation liquor is composed according to the proportion of 2:1:2: 1.
Example 5:
the inventionThe composite microbial fertilizer comprises, by weight, 25 parts of flower soil, 15 parts of potato residue, 30 parts of grass peat, 10 parts of charcoal and 20 parts of liquid microbial inoculum. The liquid microbial inoculum is prepared from bacillus subtilisBacillus subtilisVGP004 strain fermentation liquor and bacillus megateriumBacillus megaterium VGP625 strain fermentation liquor and azotobacter chroococcumAzotobacter chroococcum VGP021 strain fermentation liquid and Brevibacillus brevisBrevibacillus brevis The VGP123 strain fermentation liquor is composed according to the proportion of 4:3:2: 1.
Example 6:
the invention provides a compound microbial fertilizer which comprises, by weight, 35 parts of flower soil, 5 parts of potato residues, 20 parts of grass peat, 20 parts of charcoal and 20 parts of liquid microbial inoculum. The liquid microbial inoculum is prepared from bacillus subtilisBacillus subtilisVGP004 strain fermentation liquor and bacillus megateriumBacillus megaterium VGP625 strain fermentation liquor and azotobacter chroococcumAzotobacter chroococcum VGP021 strain fermentation liquid and Brevibacillus brevisBrevibacillus brevis The VGP123 strain fermentation liquor is composed according to the proportion of 3:3:2: 2.
The preparation method of the vegetable rhizosphere compound microbial fertilizer in the embodiments 4-6 comprises the following steps: (1) drying flower soil, potato residue, grass peat, charcoal and other raw materials respectively, removing impurities, pulverizing, and sieving with a 2mm sieve; (2) fully and uniformly mixing raw materials such as flower soil, potato residues, turf, charcoal and the like according to the weight ratio of 6:2:5: 3; (3) sterilizing the above mixed carrier at 121 deg.C and 0.1MPa for 1min, and cooling; (4) preparing NA liquid culture medium, inoculating 4 strains described in claim 2, culturing at 30 ℃ for 24-48 h, counting the number of thalli in the culture medium under a microscope by using a blood counting plate, wherein the number of thalli exceeds 109Terminating the culture at cfu/ml, and fully and uniformly mixing 4 bacterial solutions; (5) and (3) mixing the cultured bacterial liquid according to the carrier: inoculating the bacterial liquid to a sterilized carrier in a ratio of 8:2, and culturing at 28-30 ℃ for 5 days under an aseptic condition to obtain the special composite bacterial fertilizer for the vegetables. The inoculant prepared by the method is stored at room temperature, and the viable count of the inoculant after 6 months is detected to be 5.15 multiplied by 108cfu/g. Is in line with ChinaThe agricultural industry Standard of the people's republic of China (NY/T1113-2006).
The vegetable rhizosphere compound microbial fertilizer provided by the embodiments 4-6 is applied to tomatoes, Chinese cabbages and cucumbers respectively (the test places are located in Lanzhou city, Gansu province) through field experiments, and compared with CK (blank control) and various effects of the existing commercial bacillus subtilis bacterial fertilizer.
Table 2 shows the comparison between the application effects of the special microbial compound fertilizer for vegetables provided by the present invention and the application effects of the blank (no fertilizer application) and the conventional fertilizer, which are specifically as follows:
in table 2, compared with the CK blank (no fertilizer application, each index is 100%), the total yield of the tomatoes using the composite bacterial fertilizer of the invention is increased by 15.2%, the plant height is increased by 7.6%, the weight of a single fruit is increased by 3.5% on average, and the maturation period is advanced by 4 days; the total yield of the Chinese cabbage is increased by 9.4 percent, the plant height is increased by 3.4 percent, and the weight of a single plant is increased by 1.8 percent on average by using the composite bacterial fertilizer; the total yield of the cucumber using the compound bacterial fertilizer is increased by 11.7%, the plant height is increased by 8.5%, the weight of a single fruit is increased by 5.6% on average, and the maturation period is advanced by 5 days.
Compared with the commercial bacillus subtilis bacterial fertilizer, the total yield of the tomatoes using the composite bacterial fertilizer is increased by 10.2 percent, the plant height is increased by 4.9 percent, the weight of a single fruit is increased by 2.4 percent on average, and the maturation period is advanced by 3 days; the total yield of the Chinese cabbage is increased by 7.6 percent, the plant height is increased by 2.2 percent, and the weight of a single plant is increased by 1.2 percent on average by using the composite bacterial fertilizer; the total yield of the cucumber using the compound bacterial fertilizer is increased by 9.1%, the plant height is increased by 6.3%, the weight of a single fruit is increased by 4.0% on average, and the maturation period is advanced by 4 days.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. The special microbial compound fertilizer for the vegetables is characterized by being prepared from the following components in parts by weight: 60-100 parts of carrier and 15-25 parts of composite liquid microbial inoculum.
2. The special microbial compound fertilizer for vegetables as claimed in claim 1, wherein the compound liquid microbial inoculum comprises any one or more of bacillus subtilis VGP004 strain fermentation broth, bacillus megaterium VGP625 strain fermentation broth, azotobacter chroococcum VGP021 strain fermentation broth and bacillus brevis VGP123 strain fermentation broth.
3. The special microbial compound fertilizer for vegetables as claimed in claim 2, wherein the bacillus subtilis VGP004 strain is preserved in China center for type culture Collection with the preservation number of CCTCC NO: m2021578.
4. The special microbial compound fertilizer for vegetables as claimed in claim 2, wherein the bacillus megaterium VGP625 strain is preserved in China center for type culture Collection with the preservation number of CCTCC NO: m2021583.
5. The special microbial compound fertilizer for vegetables as claimed in claim 2, wherein the azotobacter chroococcum VGP021 strain is preserved in China center for type culture Collection with the preservation number of CCTCC NO: m2021584.
6. The special microbial compound fertilizer for vegetables as claimed in claim 2, wherein the brevibacillus brevis VGP123 strain is preserved in China center for type culture Collection with the preservation number of CCTCC NO: m2021581.
7. The special microbial compound fertilizer for vegetables as claimed in any one of claims 1-6, wherein in the compound liquid microbial agent, by weight, 20-40 parts of Bacillus subtilis VGP004 strain fermentation broth, 10-30 parts of Bacillus megaterium VGP625 strain fermentation broth, 10-30 parts of azotobacter chroococcum VGP021 strain fermentation broth, and 10-30 parts of Brevibacillus brevis VGP123 strain fermentation broth.
8. The special microbial compound fertilizer for vegetables as claimed in claim 7, wherein the number of thalli in the compound liquid microbial inoculum is more than or equal to 109cfu/mL; the strain culture solution is NA liquid culture medium.
9. The special microbial compound fertilizer for vegetables as claimed in claim 8, wherein the carrier is prepared from the following raw materials in parts by weight: 25-35 parts of flower soil, 5-15 parts of potato residue, 20-30 parts of grass carbon and 10-20 parts of charcoal; the carrier raw materials are crushed and then screened by a 2mm sieve.
10. A method for preparing a special microbial compound fertilizer for vegetables, which is characterized in that the special microbial compound fertilizer for vegetables is the microbial compound fertilizer as claimed in any one of claims 1 to 9, and the preparation method comprises the following steps:
s1, respectively and fully drying carrier raw materials in the sun, removing impurities, crushing, sieving and fully and uniformly mixing according to a ratio;
s2, sterilizing the uniformly mixed carrier at high temperature and high pressure, and cooling for later use;
s3, preparing NA liquid culture medium, respectively inoculating bacterial strains and culturing, wherein the quantity of the bacterial strains is more than or equal to 109Terminating the culture when cfu/mL, and uniformly mixing the bacterial liquid to obtain a composite liquid microbial inoculum;
s4, inoculating the composite liquid microbial inoculum into the sterilized carrier according to the proportion, and culturing the composite liquid microbial inoculum under an aseptic condition for use or placing the composite liquid microbial inoculum in an aseptic sealed container for normal-temperature storage for later use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110855076.2A CN113402327B (en) | 2021-07-28 | 2021-07-28 | Special microbial compound fertilizer for vegetables and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110855076.2A CN113402327B (en) | 2021-07-28 | 2021-07-28 | Special microbial compound fertilizer for vegetables and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113402327A true CN113402327A (en) | 2021-09-17 |
| CN113402327B CN113402327B (en) | 2023-02-03 |
Family
ID=77687840
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110855076.2A Active CN113402327B (en) | 2021-07-28 | 2021-07-28 | Special microbial compound fertilizer for vegetables and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113402327B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114149287A (en) * | 2021-10-27 | 2022-03-08 | 哈尔滨师范大学 | Preparation method and application of solid microbial fertilizer |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101239847A (en) * | 2008-03-07 | 2008-08-13 | 山东省农业科学院土壤肥料研究所 | Liquid composite microbial fertilizer and its preparation method |
| CN101659932A (en) * | 2009-09-18 | 2010-03-03 | 南京农业大学 | Antagonistic bacteria preventing and removing continuous cropping tobacco bacterial wilt and microbial organic fertilizer thereof |
| CN102153402A (en) * | 2010-12-15 | 2011-08-17 | 沈阳科丰牧业科技有限公司 | Liquid complex microorganism bacterial manure and preparation method thereof |
| CN102965306A (en) * | 2012-11-09 | 2013-03-13 | 中华人民共和国潍坊出入境检验检疫局 | Bacillus subtilis and application of same in resisting aspergillus |
| CN104478521A (en) * | 2014-12-20 | 2015-04-01 | 菏泽市百花生物科技有限公司 | Organic fertilizer |
| CN104628461A (en) * | 2014-12-23 | 2015-05-20 | 天长市高新技术创业服务中心 | Special compound fertilizer for cucumbers |
| CN106187345A (en) * | 2016-07-25 | 2016-12-07 | 甘肃省农业科学院土壤肥料与节水农业研究所 | A kind of Radix Angelicae Sinensis microbial fertilizer special and preparation method thereof |
| CN108586035A (en) * | 2018-04-28 | 2018-09-28 | 广西中农富玉国际农业科技有限公司 | A kind of melon water planting formula |
| CN109081757A (en) * | 2018-08-14 | 2018-12-25 | 深圳市三林生物科技工程有限公司 | Biological organic fertilizer and preparation method thereof |
| CN111542507A (en) * | 2017-08-09 | 2020-08-14 | 皮沃特生物股份有限公司 | Methods and compositions for improving engineered microorganisms |
| CN112980738A (en) * | 2021-04-09 | 2021-06-18 | 中国科学院沈阳应用生态研究所 | Compound microbial fertilizer for preventing and treating root-knot nematode and preparation method thereof |
-
2021
- 2021-07-28 CN CN202110855076.2A patent/CN113402327B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101239847A (en) * | 2008-03-07 | 2008-08-13 | 山东省农业科学院土壤肥料研究所 | Liquid composite microbial fertilizer and its preparation method |
| CN101659932A (en) * | 2009-09-18 | 2010-03-03 | 南京农业大学 | Antagonistic bacteria preventing and removing continuous cropping tobacco bacterial wilt and microbial organic fertilizer thereof |
| CN102153402A (en) * | 2010-12-15 | 2011-08-17 | 沈阳科丰牧业科技有限公司 | Liquid complex microorganism bacterial manure and preparation method thereof |
| CN102965306A (en) * | 2012-11-09 | 2013-03-13 | 中华人民共和国潍坊出入境检验检疫局 | Bacillus subtilis and application of same in resisting aspergillus |
| CN104478521A (en) * | 2014-12-20 | 2015-04-01 | 菏泽市百花生物科技有限公司 | Organic fertilizer |
| CN104628461A (en) * | 2014-12-23 | 2015-05-20 | 天长市高新技术创业服务中心 | Special compound fertilizer for cucumbers |
| CN106187345A (en) * | 2016-07-25 | 2016-12-07 | 甘肃省农业科学院土壤肥料与节水农业研究所 | A kind of Radix Angelicae Sinensis microbial fertilizer special and preparation method thereof |
| CN111542507A (en) * | 2017-08-09 | 2020-08-14 | 皮沃特生物股份有限公司 | Methods and compositions for improving engineered microorganisms |
| CN108586035A (en) * | 2018-04-28 | 2018-09-28 | 广西中农富玉国际农业科技有限公司 | A kind of melon water planting formula |
| CN109081757A (en) * | 2018-08-14 | 2018-12-25 | 深圳市三林生物科技工程有限公司 | Biological organic fertilizer and preparation method thereof |
| CN112980738A (en) * | 2021-04-09 | 2021-06-18 | 中国科学院沈阳应用生态研究所 | Compound microbial fertilizer for preventing and treating root-knot nematode and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114149287A (en) * | 2021-10-27 | 2022-03-08 | 哈尔滨师范大学 | Preparation method and application of solid microbial fertilizer |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113402327B (en) | 2023-02-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2020101394A4 (en) | Microbial fertilizer with double effects of fertilization and disease-resistance, and preparation method and use thereof | |
| KR101016859B1 (en) | Antagonistic bacteria and its microbial organic fertilizers to prevent the wilting of serial bananas | |
| EP2838988B1 (en) | Use of synergistic microorganisms and nutrients to produce signals that facilitate the germination and plant root colonization of mycorrhizal fungi in phosphorus rich environments | |
| CN101870608B (en) | Continuous cropping resisting biological organic and inorganic compound fertilizer and production method thereof | |
| KR101669599B1 (en) | Composition for eliminating odor and heavy metal comprising effective microorganism culture broth as effective component | |
| CN101870609B (en) | Phosphorus and calcium type efficient and refined multiple-strain microbial organic fertilizer and preparation method thereof | |
| CN104447001A (en) | Special compound microbial fertilizer for rice | |
| CN109650986B (en) | Carbon-based bio-organic fertilizer for improving degraded vegetable field soil and application thereof | |
| CN104447117B (en) | A kind of Fructus Vitis viniferae Special compound microbial fertilizer | |
| CN104387200A (en) | Special compound microbial fertilizer of corns | |
| KR101670651B1 (en) | Method for producing fermented liquid fertilizer of manure with reduced odor and heavy metal content using effective microorganism | |
| CN111574283A (en) | Microbial fertilizer and preparation method thereof | |
| CN107915555A (en) | A kind of slow-release fertilizer for preventing the salinization of soil and preparation method thereof | |
| CN112694370A (en) | Bio-organic fertilizer for improving stress resistance of crops and preparation method and application thereof | |
| CN109400401A (en) | A kind of preparation method and application of the new and effective composite microbic bacterial fertilizer of suitable various crop growth | |
| CN113812327B (en) | Vegetable seedling substrate based on waste utilization and preparation method thereof | |
| CN111235064A (en) | Agricultural compound microbial agent and application thereof | |
| CN113402327B (en) | Special microbial compound fertilizer for vegetables and preparation method thereof | |
| CN108034608B (en) | Bacillus methylotrophicus strain L-39 and application thereof | |
| CN110122485A (en) | A kind of trichoderma, Trichoderma and preparation method thereof | |
| CN104988094A (en) | Method for manufacturing quinclorac solid degrading inoculant | |
| CN111849829A (en) | Compound microbial agent and preparation method and application thereof | |
| CN102618527A (en) | Method for recycling production of paenibacillus polymyxa microbial agent from citric acid wastewater | |
| CN111418611A (en) | Growth-promoting and disease-preventing microbial agent special for barley crops and preparation method and application thereof | |
| CN110540461A (en) | Composite microbial liquid fertilizer for preventing and treating strawberry anthracnose as well as preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |