CN106187345A - A kind of Radix Angelicae Sinensis microbial fertilizer special and preparation method thereof - Google Patents
A kind of Radix Angelicae Sinensis microbial fertilizer special and preparation method thereof Download PDFInfo
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- CN106187345A CN106187345A CN201610585180.3A CN201610585180A CN106187345A CN 106187345 A CN106187345 A CN 106187345A CN 201610585180 A CN201610585180 A CN 201610585180A CN 106187345 A CN106187345 A CN 106187345A
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- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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Abstract
The present invention relates to bio-organic fertilizer production technical field, be specifically related to a kind of Radix Angelicae Sinensis microbial fertilizer special and preparation method thereof.Including fertilizer, weathered coal and organic carrier, fertilizer is excrement of animals, organic carrier contains Brassica campestris L pseudomonas fermentation liquid and wall fermentation of bacillus liquid, and above-mentioned substance is formulated by following weight portion: excrement of animals 50～80 parts, weathered coal 20～60 parts, Brassica campestris L pseudomonas fermentation liquid 5～15 parts, wall fermentation of bacillus liquid 5～15 parts.On Weiyuan, Gansu, Minxian County, the west of Gansu Province and other places, through the contrast test of fertilizer efficiency of 3 years, result shows: the microbial manure that this technical scheme produces is used on Radix Angelicae Sinensis has significant anti-soil-borne disease and effect of increasing production, is 45%～48% to angelica root maize ear rot prevention effect, increases production 12%～16%.
Description
Technical field
The present invention relates to bio-organic fertilizer production technical field, be specifically related to a kind of Radix Angelicae Sinensis microbial fertilizer special and
Preparation method.
Background technology
Since oneth century, commercial production high development causes air, soil pollution, and plant husbandry utilizes pesticide, change in a large number
The destruction of production environment is grown with each passing day by fertile, hormone, holds along with country " combines, comprehensive control " implementing of building policy with supporting
OK, people have a profound understanding of soil protection in production practices, the importance preserved the ecological environment and urgency, " Soil conservation and fertilization "
Start " green engineering " to implement integrally, use have that the bio-feritlizer of environmental protection and health care become agricultural development must be by it
Road.
Radix Angelicae Sinensis is grown in China's western region and generally plants in the area of height above sea level higher (1700～3000 meters), has happiness cold
Cool dark and damp feature, is a kind of low temperature long-day plant.Cultivation resource is distributed mainly on Gansu, Yunnan, Sichuan, Hubei etc. and saves
District, additionally also there is introducing and planting in Shaanxi, Ningxia, Shanxi and Guizhou.Gansu is the major production areas that successive dynasties Radix Angelicae Sinensis is genuine, cultivation history
The longest, there are about the history of more than 1700 year, the from ancient times to the present differentiation of Radix Angelicae Sinensis popular name i.e. XIGUI-Radix Angelicae Sinensis-Min returns, deep and bright
Overview works as the genuine traditional product being classified as Gansu, and Gansu Angelica is pushed away haunt and plague by successive dynasties physician, is profuse in praise, at home and abroad wins
Obtained the highest reputation.Radix Angelicae Sinensis in the long-term cultivated area of our province more than 490,000 mu, yield more than 9.5 ten thousand tons, occupy complete
More than the 80% of state's market circulation amount, mainly originates in the ground such as Minxian County, Dangchang, Zhang County, Weiyuan, Lintan.Along with opening of Radix Angelicae Sinensis new product
Send out with application, people the health demand of natural prodcuts and the developing of overseas market, the market demand to Radix Angelicae Sinensis in recent decades
Measuring increasing, its market potential also can deeply be excavated, and has a extensive future.Therefore, peasant planting enthusiasm is the highest so that cultivation
Area constantly expands, and the continuous cropping plantation causing Radix Angelicae Sinensis is more and more extensive.Continuous cropping for many years causes adding year by year of Radix Angelicae Sinensis pest and disease damage
Weight, moral character degenerate, quality decline, low yield not before, drastically influence the sustainable development of Radix Angelicae Sinensis industry.Many scholar's research
Point out: " soil-borne disease is to cause the topmost factor of Radix Angelicae Sinensis continuous cropping obstacle ", wherein root rot is that the most serious disease occurs
One of, causing root yield and quality degradation, have a strong impact on yield and the quality of genuine medicinal materials Radix Angelicae Sinensis, plantation efficiency is notable
Decline, cause extreme loss to medicinal herb grower, the serious contusion enthusiasm of medicinal herb grower.Additionally, Radix Angelicae Sinensis producing region is distributed in High aititude mostly
Remote mountain areas, production technology is the most backward, and the preventing and treating of Chinese crude drug disease is realized weakness by peasant, blindly uses pesticide, causes working as
Return the staggered frequently generation of disease large area such as root rot, fiber crops stomatosis, and in ascendant trend year by year, it has also become Genuine producing area Radix Angelicae Sinensis is raw
The key constraints produced.The outer research to Radix Angelicae Sinensis of Present Domestic focuses primarily upon pharmacological effect, preparation extracts and chemical composition
Research, the research to Radix Angelicae Sinensis root disease is less, especially root rot Biological control is not yet had system in-depth study, the most right
Preventing and treating in this disease is also compared blindly, and not only prevention effect is undesirable, also results in serious problem of environmental pollution, and to medicine
Material quality also brings harmful effect, does not meets Producing medicinal herbs management regulation requirement.By developing a kind of micro-life of new type functional
Thing fertilizer, carries out seedling seed dressing or spreads manuer in holes process, can cut off the propagation of pathogenic bacteria from source, for delaying Drug resistance produce and carry
High chemical prevention dynamics provides effective and ensures, is the urgent needs promoting Radix Angelicae Sinensis industry fast-developing further.
Summary of the invention
The purpose of the present invention is aiming at above-mentioned defect of the prior art, it is provided that one can effectively overcome continuous cropping to cause
The Radix Angelicae Sinensis bio-organic fertilizer special material of root rot penalty issue and preparation method thereof.
Radix Angelicae Sinensis microbial fertilizer special, including fertilizer, weathered coal and organic carrier, fertilizer is excrement of animals, organic
Carrier contains Brassica campestris L pseudomonas fermentation liquid and wall fermentation of bacillus liquid, and above-mentioned substance is formulated by following weight portion: domestic animal
Animal manure just 50～80 parts, weathered coal 20～60 parts, Brassica campestris L pseudomonas fermentation liquid 5～15 parts, wall fermentation of bacillus liquid 5～15
Part.
Formulated by following weight portion: excrement of animals 50～60 parts, weathered coal 30～50 parts, the fermentation of Brassica campestris L pseudomonas
Liquid 6～12 parts, wall fermentation of bacillus liquid 6～12 parts.
Formulated by following weight portion: excrement of animals 50 parts, weathered coal 50 parts, Brassica campestris L pseudomonas fermentation liquid 7.5 parts,
Wall fermentation of bacillus liquid 7.5 parts.
Described excrement of animals is sheep stool, cattle manure or the mixture of the two.
Described Brassica campestris L pseudomonas fermentation liquid, wall fermentation of bacillus liquid are through two-stage fermentation, wherein
One-level Brassica campestris L pseudomonas fermentation medium components: Carnis Bovis seu Bubali cream 3g, peptone 10g, sodium chloride 5g, water 1000ml, uses hydrogen
Sodium oxide regulation pH value 7.2～7.4, the fermentation condition of one-level Brassica campestris L pseudomonas: ventilation is 4.5NL/min, mixing speed
300r/min, cultivation temperature 30 DEG C, incubation time is 18～24 hours；
Two grades of Brassica campestris L pseudomonas expanding propagation strain fermentation medium components: glucose 20 g, peptone 10g, dipotassium hydrogen phosphate 1g,
Potassium dihydrogen phosphate 1g, Magnesium sulfate heptahydrate 0.5g, sodium chloride 1g, calcium chloride 1 g, water 1000ml, with sodium hydroxide or hydrochloric acid
Regulation pH value 7.0～7.2, two grades of Brassica campestris L pseudomonas expanding propagation strain fermentation conditions: ventilation is 4.5NL/min, mixing speed
300r/min, cultivation temperature, 30～33 DEG C, incubation time is 24～36 hours；
One-level wall fermentation of bacillus medium component: Carnis Bovis seu Bubali cream 3g, peptone 10g, sodium chloride 5g, water 1000ml, use hydrogen-oxygen
Change sodium regulation pH value 7.2～7.4, one-level wall fermentation of bacillus condition: ventilation is 4.5NL/min, mixing speed 300r/
Min, cultivation temperature 30 DEG C, incubation time is 24～48 hours；
Two grades of wall bacillus cereus expanding propagation strain fermentation medium components: glucose 5g, starch 3g, Semen sojae atricolor powder 20g, yeast extract 0.2
G, dipotassium hydrogen phosphate 3g, potassium dihydrogen phosphate 1.5g, manganese sulfate 0.006g, Magnesium sulfate heptahydrate 0.5g, ferric chloride 0.1g, water
1000ml, with sodium hydroxide or salt acid for adjusting pH value 7.0～7.2, two grades of wall bacillus cereus expanding propagation strain fermentation conditions: ventilation
Amount is 4.5NL/min, and mixing speed 300r/min, cultivation temperature 30 DEG C, incubation time is 72～96 hours.
The preparation method of Radix Angelicae Sinensis microbial fertilizer special, comprises the steps:
A, stacking ferment
Excrement of animals and weathered coal mixing heap make buttress, the interior temperature 50 C of stacking ferment control bar buttress～70 DEG C, carry out every 2 days
Turning, through the fermentation of 20～30 days；
B, mixed fermentation
After the material of step A is mixed homogeneously with Brassica campestris L pseudomonas fermentation liquid, wall fermentation of bacillus liquid, standing for fermentation 7～
10 days, obtain Radix Angelicae Sinensis microbial fertilizer special.
Material after described step A fermentation ends carry out air-dry process moisture content to 10-15%, pulverized 80 mesh sieves.
Excrement of animals and weathered coal are mixed heap and make buttress, wide 1.8～3 meters, high 1.2～1.5 meters, length by described step A
Bar buttress more than or equal to 2 meters.
Described step B standing for fermentation 7～10 days under conditions of 18 DEG C～30 DEG C.
The invention have the benefit that
1, the adding proportion of fertilizer, weathered coal and organic carrier in claims 1 to 3, it is ensured that the acid-base value of fertilizer exists
Between 6.9-7.2, close with the second order fermentation product pH value of organic carrier.Integral production operation pH value tends towards stability, and is only having
Finely tune as required during airborne body second order fermentation.Under above-mentioned pH value, the content of organic matter is higher, viable count is higher, second order fermentation
In two kinds of viable counts be above 200,000,000.Product quality easily reaches in the industry standard NY/T 798-2015 of composite microbiological fertilizer
Regulation.
2, carry out the advantage of bar buttress formula fermentation according to 50 parts of excrements of animals of weight ratio and the mixing of 50 parts of weathered coals and be feces
In nitrogen loss few, ammonia volatilization is low, and environmental pollution is the lightest.
3, application two-stage fermentation technique fermentation, fermentation time is short, and viable count is high, and spore production is high.2 kinds of strain one grade fermemtations
Seed culture fluid uses general beef extract-peptone, and formula is simple, has versatility, and second order fermentation uses unique joining
Side, Brassica campestris L pseudomonas strain second order fermentation culture fluid, produce number of viable many, bacterium solution anti-Radix Angelicae Sinensis pine root fungus ability is strong, wall bud
Spore bacillus species second order fermentation culture fluid, produces number of viable many, and spore conversion ratio is high, and more than 90%.
Diseases prevention Radix Angelicae Sinensis microbial fertilizer special that the present invention provides in sum and preparation method thereof, for Radix Angelicae Sinensis road real estate
District's crop varieties is single, and the staggered frequently generations of disease large area such as the angelica root maize ear rot generally caused, numb stomatosis are planted in continuous cropping
Problem, solves the soil-borne disease penalty issue that continuous cropping causes, and under conditions of reducing pesticide, fertilizer amount, improves Radix Angelicae Sinensis yield,
By adding two kinds of organic carriers, not only promoted to grow but also improve resistance against diseases.Take full advantage of agriculture and animal husbandry waste resource, decrease ring
The pollution in border, solves the support material problem produced needed for Radix Angelicae Sinensis microbial fertilizer special；
The present invention produces Radix Angelicae Sinensis microbial fertilizer special, on Weiyuan, Gansu, Minxian County, the west of Gansu Province and other places, through the fertilizer efficiency of 3 years to having a competition
Testing, result shows: the microbial manure that this technical scheme produces is used on Radix Angelicae Sinensis has significant anti-soil-borne disease and volume increase effect
Really, it is 45%～48% to angelica root maize ear rot prevention effect, increases production 12%～16%.
Detailed description of the invention
Radix Angelicae Sinensis microbial fertilizer special, it is characterised in that include fertilizer, weathered coal and organic carrier, fertilizer is domestic animal
Feces, organic carrier contains Brassica campestris L pseudomonas fermentation liquid and wall fermentation of bacillus liquid, and above-mentioned substance is joined by following weight portion
System forms: excrement of animals 50～80 parts, weathered coal 20～60 parts, Brassica campestris L pseudomonas fermentation liquid 5～15 parts, wall bacillus cereus are sent out
Ferment liquid 5～15 parts.
Preferably formulated by following weight portion: excrement of animals 50～60 parts, weathered coal 30～50 parts, Brassica campestris L vacation unit cell
Fermented liquid 6～12 parts, wall fermentation of bacillus liquid 6～12 parts.
Preferably formulated by following weight portion: excrement of animals 50 parts, weathered coal 50 parts, Brassica campestris L pseudomonas fermentation liquid
7.5 parts, wall fermentation of bacillus liquid 7.5 parts.
Described excrement of animals is sheep stool, cattle manure or the mixture of the two.
Described Brassica campestris L pseudomonas fermentation liquid, wall fermentation of bacillus liquid are through two-stage fermentation, wherein
One-level Brassica campestris L pseudomonas fermentation medium components: Carnis Bovis seu Bubali cream 3g, peptone 10g, sodium chloride 5g, water 1000ml, uses hydrogen
Sodium oxide regulation pH value 7.2～7.4, the fermentation condition of one-level Brassica campestris L pseudomonas: ventilation is 4.5NL/min, mixing speed
300r/min, cultivation temperature 30 DEG C, incubation time is 18～24 hours；
Two grades of Brassica campestris L pseudomonas expanding propagation strain fermentation medium components: glucose 20 g, peptone 10g, dipotassium hydrogen phosphate 1g,
Potassium dihydrogen phosphate 1g, Magnesium sulfate heptahydrate 0.5g, sodium chloride 1g, calcium chloride 1 g, water 1000ml, with sodium hydroxide or hydrochloric acid
Regulation pH value 7.0～7.2, two grades of Brassica campestris L pseudomonas expanding propagation strain fermentation conditions: ventilation is 4.5NL/min, mixing speed
300r/min, cultivation temperature, 30～33 DEG C, incubation time is 24～36 hours；
One-level wall fermentation of bacillus medium component: Carnis Bovis seu Bubali cream 3g, peptone 10g, sodium chloride 5g, water 1000ml, use hydrogen-oxygen
Change sodium regulation pH value 7.2～7.4, one-level wall fermentation of bacillus condition: ventilation is 4.5NL/min, mixing speed 300r/
Min, cultivation temperature 30 DEG C, incubation time is 24～48 hours；
Two grades of wall bacillus cereus expanding propagation strain fermentation medium components: glucose 5g, starch 3g, Semen sojae atricolor powder 20g, yeast extract 0.2
G, dipotassium hydrogen phosphate 3g, potassium dihydrogen phosphate 1.5g, manganese sulfate 0.006g, Magnesium sulfate heptahydrate 0.5g, ferric chloride 0.1g, water
1000ml, with sodium hydroxide or salt acid for adjusting pH value 7.0～7.2, two grades of wall bacillus cereus expanding propagation strain fermentation conditions: ventilation
Amount is 4.5NL/min, and mixing speed 300r/min, cultivation temperature 30 DEG C, incubation time is 72～96 hours.
The preparation method of Radix Angelicae Sinensis microbial fertilizer special, comprises the steps:
A, stacking ferment
Excrement of animals and weathered coal mixing heap make buttress, wide 1.8～3 meters, high 1.2～1.5 meters, the length bar more than or equal to 2 meters
Buttress, the interior temperature 50 C of stacking ferment control bar buttress～70 DEG C, carried out turning every 2 days, through the fermentation of 20～30 days；Fermentation knot
Material after bundle carry out air-dry process moisture content to 10-15%, pulverized 80 mesh sieves.
B, mixed fermentation
After the material of step A is mixed homogeneously with Brassica campestris L pseudomonas fermentation liquid, wall fermentation of bacillus liquid, at 18 DEG C～30 DEG C
Under conditions of standing for fermentation 7～10 days, obtain Radix Angelicae Sinensis microbial fertilizer special.
Material used in the present invention is originated:
Brassica campestris L pseudomonas and wall Bacillus: prepare the most voluntarily.
Manure of livestock and poultry: sheep raising field or vaccary are purchased.
Weathered coal: buy from Tian Wang fertilizer company limited of Wuhai of Inner Mongolia.
Embodiment 1:
Radix Angelicae Sinensis microbial fertilizer special, it is characterised in that include fertilizer, weathered coal and organic carrier, fertilizer is animal manure
Just, organic carrier contains Brassica campestris L pseudomonas fermentation liquid and wall fermentation of bacillus liquid, and above-mentioned substance is prepared by following weight portion
Form: excrement of animals 50 parts, weathered coal 20 parts, Brassica campestris L pseudomonas fermentation liquid 5 parts, wall fermentation of bacillus liquid 5 parts.Described domestic animal
Animal manure is just sheep stool, cattle manure or the mixture of the two.
Described Brassica campestris L pseudomonas fermentation liquid, wall fermentation of bacillus liquid are through two-stage fermentation, wherein
One-level Brassica campestris L pseudomonas fermentation medium components: Carnis Bovis seu Bubali cream 3g, peptone 10g, sodium chloride 5g, water 1000ml, uses hydrogen
Sodium oxide regulation pH value 7.2～7.4, the fermentation condition of one-level Brassica campestris L pseudomonas: ventilation is 4.5NL/min, mixing speed
300r/min, cultivation temperature 30 DEG C, incubation time is 18～24 hours；
Two grades of Brassica campestris L pseudomonas expanding propagation strain fermentation medium components: glucose 20 g, peptone 10g, dipotassium hydrogen phosphate 1g,
Potassium dihydrogen phosphate 1g, Magnesium sulfate heptahydrate 0.5g, sodium chloride 1g, calcium chloride 1 g, water 1000ml, with sodium hydroxide or hydrochloric acid
Regulation pH value 7.0～7.2, two grades of Brassica campestris L pseudomonas expanding propagation strain fermentation conditions: ventilation is 4.5NL/min, mixing speed
300r/min, cultivation temperature, 30～33 DEG C, incubation time is 24～36 hours；
One-level wall fermentation of bacillus medium component: Carnis Bovis seu Bubali cream 3g, peptone 10g, sodium chloride 5g, water 1000ml, use hydrogen-oxygen
Change sodium regulation pH value 7.2～7.4, one-level wall fermentation of bacillus condition: ventilation is 4.5NL/min, mixing speed 300r/
Min, cultivation temperature 30 DEG C, incubation time is 24～48 hours；
Two grades of wall bacillus cereus expanding propagation strain fermentation medium components: glucose 5g, starch 3g, Semen sojae atricolor powder 20g, yeast extract 0.2
G, dipotassium hydrogen phosphate 3g, potassium dihydrogen phosphate 1.5g, manganese sulfate 0.006g, Magnesium sulfate heptahydrate 0.5g, ferric chloride 0.1g, water
1000ml, with sodium hydroxide or salt acid for adjusting pH value 7.0～7.2, two grades of wall bacillus cereus expanding propagation strain fermentation conditions: ventilation
Amount is 4.5NL/min, and mixing speed 300r/min, cultivation temperature 30 DEG C, incubation time is 72～96 hours.
The preparation method of Radix Angelicae Sinensis microbial fertilizer special, comprises the steps:
A, stacking ferment
Excrement of animals and weathered coal mixing heap make buttress, wide 1.8 meters, high 1.2 meters, the length bar buttress equal to 2 meters, stacking fermentation control
Temperature 50 C～70 DEG C in bar processed buttress, carried out turning every 2 days, through the fermentation of 20 days；Material after fermentation ends carries out wind
Dry-cure moisture content, to 10%, pulverized 80 mesh sieves.
B, mixed fermentation
After the material of step A is mixed homogeneously with Brassica campestris L pseudomonas fermentation liquid, wall fermentation of bacillus liquid, at 18 DEG C～30 DEG C
Under conditions of standing for fermentation 7 days, obtain Radix Angelicae Sinensis microbial fertilizer special.
Embodiment 2:
Radix Angelicae Sinensis microbial fertilizer special, it is characterised in that include fertilizer, weathered coal and organic carrier, fertilizer is animal manure
Just, organic carrier contains Brassica campestris L pseudomonas fermentation liquid and wall fermentation of bacillus liquid, and above-mentioned substance is prepared by following weight portion
Form: excrement of animals 80 parts, weathered coal 60 parts, Brassica campestris L pseudomonas fermentation liquid 15 parts, wall fermentation of bacillus liquid 15 parts.
Described excrement of animals is sheep stool, cattle manure or the mixture of the two.
Described Brassica campestris L pseudomonas fermentation liquid, wall fermentation of bacillus liquid are through two-stage fermentation, wherein
One-level Brassica campestris L pseudomonas fermentation medium components: Carnis Bovis seu Bubali cream 3g, peptone 10g, sodium chloride 5g, water 1000ml, uses hydrogen
Sodium oxide regulation pH value 7.2～7.4, the fermentation condition of one-level Brassica campestris L pseudomonas: ventilation is 4.5NL/min, mixing speed
300r/min, cultivation temperature 30 DEG C, incubation time is 18～24 hours；
Two grades of Brassica campestris L pseudomonas expanding propagation strain fermentation medium components: glucose 20 g, peptone 10g, dipotassium hydrogen phosphate 1g,
Potassium dihydrogen phosphate 1g, Magnesium sulfate heptahydrate 0.5g, sodium chloride 1g, calcium chloride 1 g, water 1000ml, with sodium hydroxide or hydrochloric acid
Regulation pH value 7.0～7.2, two grades of Brassica campestris L pseudomonas expanding propagation strain fermentation conditions: ventilation is 4.5NL/min, mixing speed
300r/min, cultivation temperature, 30～33 DEG C, incubation time is 24～36 hours；
One-level wall fermentation of bacillus medium component: Carnis Bovis seu Bubali cream 3g, peptone 10g, sodium chloride 5g, water 1000ml, use hydrogen-oxygen
Change sodium regulation pH value 7.2～7.4, one-level wall fermentation of bacillus condition: ventilation is 4.5NL/min, mixing speed 300r/
Min, cultivation temperature 30 DEG C, incubation time is 24～48 hours；
Two grades of wall bacillus cereus expanding propagation strain fermentation medium components: glucose 5g, starch 3g, Semen sojae atricolor powder 20g, yeast extract 0.2
G, dipotassium hydrogen phosphate 3g, potassium dihydrogen phosphate 1.5g, manganese sulfate 0.006g, Magnesium sulfate heptahydrate 0.5g, ferric chloride 0.1g, water
1000ml, with sodium hydroxide or salt acid for adjusting pH value 7.0～7.2, two grades of wall bacillus cereus expanding propagation strain fermentation conditions: ventilation
Amount is 4.5NL/min, and mixing speed 300r/min, cultivation temperature 30 DEG C, incubation time is 72～96 hours.
The preparation method of Radix Angelicae Sinensis microbial fertilizer special, comprises the steps:
A, stacking ferment
Excrement of animals and weathered coal mixing heap make buttress, and wide 3 meters, high 1.5 meters, the bar buttress of long 100 meters, stacking ferment control bar is piled up neatly
Interior temperature 50 C～70 DEG C, carried out turning every 2 days, through the fermentation of 30 days；Material after fermentation ends carries out air-drying process
Moisture content, to 15%, pulverized 80 mesh sieves.
B, mixed fermentation
After the material of step A is mixed homogeneously with Brassica campestris L pseudomonas fermentation liquid, wall fermentation of bacillus liquid, at 18 DEG C～30 DEG C
Under conditions of standing for fermentation 10 days, obtain Radix Angelicae Sinensis microbial fertilizer special.
Embodiment 3:
Radix Angelicae Sinensis microbial fertilizer special, it is characterised in that include fertilizer, weathered coal and organic carrier, fertilizer is animal manure
Just, organic carrier contains Brassica campestris L pseudomonas fermentation liquid and wall fermentation of bacillus liquid, formulated by following weight portion: domestic animal
50 parts of feces, weathered coal 50 parts, Brassica campestris L pseudomonas fermentation liquid 7.5 parts, wall fermentation of bacillus liquid 7.5 parts.
Described excrement of animals is sheep stool, cattle manure or the mixture of the two.
Described Brassica campestris L pseudomonas fermentation liquid, wall fermentation of bacillus liquid are through two-stage fermentation, wherein
One-level Brassica campestris L pseudomonas fermentation medium components: Carnis Bovis seu Bubali cream 3g, peptone 10g, sodium chloride 5g, water 1000ml, uses hydrogen
Sodium oxide regulation pH value 7.2～7.4, the fermentation condition of one-level Brassica campestris L pseudomonas: ventilation is 4.5NL/min, mixing speed
300r/min, cultivation temperature 30 DEG C, incubation time is 18～24 hours；
Two grades of Brassica campestris L pseudomonas expanding propagation strain fermentation medium components: glucose 20 g, peptone 10g, dipotassium hydrogen phosphate 1g,
Potassium dihydrogen phosphate 1g, Magnesium sulfate heptahydrate 0.5g, sodium chloride 1g, calcium chloride 1 g, water 1000ml, with sodium hydroxide or hydrochloric acid
Regulation pH value 7.0～7.2, two grades of Brassica campestris L pseudomonas expanding propagation strain fermentation conditions: ventilation is 4.5NL/min, mixing speed
300r/min, cultivation temperature, 30～33 DEG C, incubation time is 24～36 hours；
One-level wall fermentation of bacillus medium component: Carnis Bovis seu Bubali cream 3g, peptone 10g, sodium chloride 5g, water 1000ml, use hydrogen-oxygen
Change sodium regulation pH value 7.2～7.4, one-level wall fermentation of bacillus condition: ventilation is 4.5NL/min, mixing speed 300r/
Min, cultivation temperature 30 DEG C, incubation time is 24～48 hours；
Two grades of wall bacillus cereus expanding propagation strain fermentation medium components: glucose 5g, starch 3g, Semen sojae atricolor powder 20g, yeast extract 0.2
G, dipotassium hydrogen phosphate 3g, potassium dihydrogen phosphate 1.5g, manganese sulfate 0.006g, Magnesium sulfate heptahydrate 0.5g, ferric chloride 0.1g, water
1000ml, with sodium hydroxide or salt acid for adjusting pH value 7.0～7.2, two grades of wall bacillus cereus expanding propagation strain fermentation conditions: ventilation
Amount is 4.5NL/min, and mixing speed 300r/min, cultivation temperature 30 DEG C, incubation time is 72～96 hours.
The preparation method of Radix Angelicae Sinensis microbial fertilizer special, comprises the steps:
A, stacking ferment
Excrement of animals and weathered coal mixing heap make buttress, and wide 2 meters, high 1.5 meters, the bar buttress of long 10 meters, stacking ferment control bar is piled up neatly
Interior temperature 50 C～70 DEG C, carried out turning every 2 days, through the fermentation of 25 days；Material after fermentation ends carries out air-drying process
Moisture content, to 10%, pulverized 80 mesh sieves.
B, mixed fermentation
After the material of step A is mixed homogeneously with Brassica campestris L pseudomonas fermentation liquid, wall fermentation of bacillus liquid, at 18 DEG C～30 DEG C
Under conditions of standing for fermentation 10 days, obtain Radix Angelicae Sinensis microbial fertilizer special.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to aforementioned reality
Executing example to be described in detail the present invention, for a person skilled in the art, it still can be to aforementioned each enforcement
Technical scheme described in example is modified, or wherein portion of techniques feature is carried out equivalent.All essences in the present invention
Within god and principle, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (9)
1. Radix Angelicae Sinensis microbial fertilizer special, it is characterised in that include fertilizer, weathered coal and organic carrier, fertilizer is animal manure
Just, organic carrier contains Brassica campestris L pseudomonas fermentation liquid and wall fermentation of bacillus liquid, and above-mentioned substance is prepared by following weight portion
Form: excrement of animals 50～80 parts, weathered coal 20～60 parts, Brassica campestris L pseudomonas fermentation liquid 5～15 parts, wall fermentation of bacillus
Liquid 5～15 parts.
Radix Angelicae Sinensis microbial fertilizer special the most according to claim 1, it is characterised in that formulated by following weight portion: domestic animal
Animal manure just 50～60 parts, weathered coal 30～50 parts, Brassica campestris L pseudomonas fermentation liquid 6～12 parts, wall fermentation of bacillus liquid 6～12
Part.
Radix Angelicae Sinensis microbial fertilizer special the most according to claim 2, it is characterised in that formulated by following weight portion: domestic animal
Animal manure just 50 parts, weathered coal 50 parts, Brassica campestris L pseudomonas fermentation liquid 7.5 parts, wall fermentation of bacillus liquid 7.5 parts.
Radix Angelicae Sinensis microbial fertilizer special the most according to claim 2, it is characterised in that described excrement of animals is sheep stool, cattle manure
Or the mixture of the two.
Radix Angelicae Sinensis microbial fertilizer special the most according to claim 4, it is characterised in that described Brassica campestris L pseudomonas fermentation liquid,
Wall fermentation of bacillus liquid is through two-stage fermentation, wherein
One-level Brassica campestris L pseudomonas fermentation medium components: Carnis Bovis seu Bubali cream 3g, peptone 10g, sodium chloride 5g, water 1000ml, uses hydrogen
Sodium oxide regulation pH value 7.2～7.4, the fermentation condition of one-level Brassica campestris L pseudomonas: ventilation is 4.5NL/min, mixing speed
300r/min, cultivation temperature 30 DEG C, incubation time is 18～24 hours；
Two grades of Brassica campestris L pseudomonas expanding propagation strain fermentation medium components: glucose 20 g, peptone 10g, dipotassium hydrogen phosphate 1g,
Potassium dihydrogen phosphate 1g, Magnesium sulfate heptahydrate 0.5g, sodium chloride 1g, calcium chloride 1 g, water 1000ml, with sodium hydroxide or hydrochloric acid
Regulation pH value 7.0～7.2, two grades of Brassica campestris L pseudomonas expanding propagation strain fermentation conditions: ventilation is 4.5NL/min, mixing speed
300r/min, cultivation temperature, 30～33 DEG C, incubation time is 24～36 hours；
One-level wall fermentation of bacillus medium component: Carnis Bovis seu Bubali cream 3g, peptone 10g, sodium chloride 5g, water 1000ml, use hydrogen-oxygen
Change sodium regulation pH value 7.2～7.4, one-level wall fermentation of bacillus condition: ventilation is 4.5NL/min, mixing speed 300r/
Min, cultivation temperature 30 DEG C, incubation time is 24～48 hours；
Two grades of wall bacillus cereus expanding propagation strain fermentation medium components: glucose 5g, starch 3g, Semen sojae atricolor powder 20g, yeast extract 0.2
G, dipotassium hydrogen phosphate 3g, potassium dihydrogen phosphate 1.5g, manganese sulfate 0.006g, Magnesium sulfate heptahydrate 0.5g, ferric chloride 0.1g, water
1000ml, with sodium hydroxide or salt acid for adjusting pH value 7.0～7.2, two grades of wall bacillus cereus expanding propagation strain fermentation conditions: ventilation
Amount is 4.5NL/min, and mixing speed 300r/min, cultivation temperature 30 DEG C, incubation time is 72～96 hours.
6. according to the preparation method of the Radix Angelicae Sinensis microbial fertilizer special described in any one of claim 1 to 5, it is characterised in that include
Following steps:
A, stacking ferment
Excrement of animals and weathered coal mixing heap make buttress, the interior temperature 50 C of stacking ferment control bar buttress～70 DEG C, carry out every 2 days
Turning, through the fermentation of 20～30 days；
B, mixed fermentation
After the material of step A is mixed homogeneously with Brassica campestris L pseudomonas fermentation liquid, wall fermentation of bacillus liquid, standing for fermentation 7～
10 days, obtain Radix Angelicae Sinensis microbial fertilizer special.
The preparation method of Radix Angelicae Sinensis microbial fertilizer special the most according to claim 6, it is characterised in that described step A is fermented
Material after end carry out air-dry process moisture content to 10-15%, pulverized 80 mesh sieves.
The preparation method of Radix Angelicae Sinensis microbial fertilizer special the most according to claim 6, it is characterised in that described step A is by domestic animal
Animal manure just makes buttress, wide 1.8～3 meters, high 1.2～1.5 meters, the length bar buttress more than or equal to 2 meters with weathered coal mixing heap.
The preparation method of Radix Angelicae Sinensis microbial fertilizer special the most according to claim 6, it is characterised in that described step B is 18
DEG C～30 DEG C under conditions of standing for fermentation 7～10 days.
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| CN201610585180.3A CN106187345A (en) | 2016-07-25 | 2016-07-25 | A kind of Radix Angelicae Sinensis microbial fertilizer special and preparation method thereof |
| CN202111261353.3A CN113880616A (en) | 2016-07-25 | 2016-07-25 | Application of microbial fertilizer in soil-borne disease resistance and/or yield increase of angelica sinensis |
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| CN201610585180.3A CN106187345A (en) | 2016-07-25 | 2016-07-25 | A kind of Radix Angelicae Sinensis microbial fertilizer special and preparation method thereof |
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| CN202111261353.3A Division CN113880616A (en) | 2016-07-25 | 2016-07-25 | Application of microbial fertilizer in soil-borne disease resistance and/or yield increase of angelica sinensis |
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| CN202111261353.3A Pending CN113880616A (en) | 2016-07-25 | 2016-07-25 | Application of microbial fertilizer in soil-borne disease resistance and/or yield increase of angelica sinensis |
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Cited By (3)
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| CN108821835A (en) * | 2018-07-20 | 2018-11-16 | 贵州珍安药业有限公司 | A kind of fertilizer special for Chinese angelica and its production method |
| CN113402327A (en) * | 2021-07-28 | 2021-09-17 | 甘肃省农业科学院植物保护研究所 | Special microbial compound fertilizer for vegetables and preparation method thereof |
| CN114181017A (en) * | 2021-12-15 | 2022-03-15 | 北京嘉博文生物科技有限公司 | Composition for relieving angelica sinensis continuous cropping obstacle and preparation method and application thereof |
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| CN105622289A (en) * | 2015-12-23 | 2016-06-01 | 成都新柯力化工科技有限公司 | Ecological fertilizer special for Chinese angelica and preparation method of ecological fertilizer |
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| CN102190524A (en) * | 2010-03-17 | 2011-09-21 | 甘肃省农业科学院土壤肥料与节水农业研究所 | Biological potassic fertilizer special for potatoes and preparation method thereof |
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| CN114181017A (en) * | 2021-12-15 | 2022-03-15 | 北京嘉博文生物科技有限公司 | Composition for relieving angelica sinensis continuous cropping obstacle and preparation method and application thereof |
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| CN113880616A (en) | 2022-01-04 |
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Application publication date: 20161207 |