CN113880616A - Application of microbial fertilizer in soil-borne disease resistance and/or yield increase of angelica sinensis - Google Patents
Application of microbial fertilizer in soil-borne disease resistance and/or yield increase of angelica sinensis Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/70—Controlling the treatment in response to process parameters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention belongs to the technical field of microbial fertilizers, and particularly relates to a special microbial fertilizer for angelica sinensis as well as a preparation method and application thereof. The microbial fertilizer comprises an organic fertilizer, weathered coal and an organic carrier, wherein the organic fertilizer is livestock manure, the organic carrier contains pseudomonas brassicae fermentation liquor and bacillus muralis fermentation liquor, and the microbial fertilizer is prepared from the following components in parts by weight: 50-80 parts of livestock manure, 20-60 parts of weathered coal, 5-15 parts of pseudomonas brassicae fermentation liquor and 5-15 parts of bacillus muralis fermentation liquor. The fertilizer is applied to angelica districts such as Weiyuan, Mingxian and Longxi, has obvious effects of resisting soil-borne diseases and increasing yield, has the effect of preventing and treating the root rot of the angelica sinensis by 45-48 percent, increases the yield by 12-16 percent and has great significance for green production of the angelica sinensis.
Description
The application is a divisional application with the application date of 2016, 25/07, application number of 201610585180.3 and the name of 'a microbial fertilizer special for angelica and a preparation method thereof'.
Technical Field
The invention belongs to the technical field of microbial fertilizers, and particularly relates to an application of a microbial fertilizer in the soil-borne disease resistance and/or yield increase of angelica.
Background
Over a century, the high development of industrial production causes air and soil pollution, the damage of the planting industry to the production environment by using pesticides, fertilizers and hormones in large quantity is increasing day by day, and the use of biological fertilizers with environmental protection and health care functions becomes a necessary way for agricultural development.
Angelica sinensis grows in western regions of China, is usually planted in regions with higher altitude (1700-3000 m), has the characteristic of preference for cold, cool, shady and humid, and is a low-temperature long-day plant. The cultivation resources are mainly distributed in Gansu, Yunnan, Sichuan, Hubei provinces and other provinces, and Shaanxi, Ningxia, Shanxi and Guizhou provinces also have introduction cultivation. Gansu is the main genuine production area of angelica in all ages, the cultivation history is the longest, the history is about 1700 years, the evolution of the custom name of angelica from ancient to present is western angelica-Qin Gui-Min Gui, the deep and well-known summary is the traditional genuine product in Gansu, and angelica in Gansu is a popular and profound product for doctors in all ages, and wins high reputation at home and abroad. The planting area of the angelica in Gansu province is over 49 million mu, the yield is over 9.5 million tons, the angelica occupies over 80 percent of the national market traffic, and the angelica is mainly produced in Min county, Dangchang, Zhang county, Weiyuan, lintan and other places. With the development and application of new angelica products, the demand of people on healthy natural products and the development of overseas markets, the demand of the markets for angelica is more and more increased in recent decades, the market potential can be deeply excavated, and the prospect is wide. Therefore, farmers have high planting enthusiasm, so that the cultivation area is continuously enlarged, and the continuous cropping planting of angelica is more and more extensive. The continuous cropping for many years leads to the gradual increase of the diseases and insect pests of the angelica, the qualitative degeneration, the quality reduction and the continuous yield wandering, and seriously influences the sustainable development of the angelica industry. Many scholars have studied to note that: the soil-borne disease is the most main factor causing the continuous cropping obstacle of the angelica, wherein the root rot is one of the most serious diseases, which causes the serious reduction of the yield and the quality of medicinal materials, seriously influences the yield and the quality of the genuine medicinal material angelica, obviously reduces the planting efficiency, causes great loss to drug-delivery farmers and seriously contusions the enthusiasm of the drug-delivery farmers. In addition, the angelica sinensis production areas are mostly distributed in remote mountainous areas at high altitude, the production technology is quite backward, the prevention and control consciousness of farmers on the Chinese medicinal material diseases is weak, the diseases such as angelica root rot, scurf and the like frequently occur in a large area in a staggered way due to blind use of pesticides, and the Chinese angelica sinensis production areas are in a trend of rising year by year and become the main limiting factor for the angelica sinensis production in real estate areas. At present, the research on angelica at home and abroad mainly focuses on the research on pharmacological efficacy, preparation extraction and chemical components, and the biological control of the angelica root rot is not researched systematically, so that the control of the angelica root rot is relatively blind, the control effect is not ideal, the problem of serious environmental pollution is caused, the quality of medicinal materials is also affected, and the requirements of the production and management specifications of the Chinese medicinal materials are not met. Through developing a novel functional microbial fertilizer, seed dressing or hole application treatment of seedlings is carried out, the propagation of germs can be cut off from the source, effective guarantee is provided for delaying the generation of drug resistance and improving the chemical prevention and control strength, and the fertilizer is an urgent need for promoting the further rapid development of the angelica industry.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides the application of the microbial fertilizer in the soil-borne disease resistance and/or yield increase of the angelica.
The invention provides an application of a microbial fertilizer in the soil-borne disease resistance and/or yield increase effect of angelica, wherein the microbial fertilizer consists of an organic fertilizer, weathered coal and an organic carrier; the organic fertilizer is livestock manure; the organic carrier is a pseudomonas brassicae fermentation liquid and a bacillus muralis fermentation liquid.
Preferably, the microbial fertilizer is prepared from the following components in parts by weight: 50-80 parts of livestock manure, 20-60 parts of weathered coal, 5-15 parts of pseudomonas brassicae fermentation liquor and 5-15 parts of bacillus muralis fermentation liquor.
Preferably, the microbial fertilizer is prepared from the following components in parts by weight: 50-60 parts of livestock manure, 30-50 parts of weathered coal, 6-12 parts of pseudomonas brassicae fermentation liquor and 6-12 parts of bacillus muralis fermentation liquor.
Preferably, the microbial fertilizer is prepared from the following components in parts by weight: 50 parts of livestock manure, 50 parts of weathered coal, 7.5 parts of pseudomonas brassicae fermentation liquor and 7.5 parts of bacillus muralis fermentation liquor.
Preferably, the livestock manure is sheep manure and/or cow manure.
Preferably, the pseudomonas brassicae fermentation liquor and the bacillus muralis fermentation liquor are subjected to two-stage fermentation, wherein,
the components of the primary rape pseudomonas fermentation medium are as follows: 3g of beef extract, 10g of peptone, 5g of sodium chloride and 1000ml of water, and adjusting the pH value to 7.2-7.4 by using sodium hydroxide; fermentation conditions of the pseudomonas brassicae: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 18-24 hours;
the components of the fermentation medium of the second-level pseudomonas brassicae propagation strain are as follows: 20g of glucose, 10g of peptone, 1g of dipotassium hydrogen phosphate, 1g of monopotassium phosphate, 0.5g of magnesium sulfate heptahydrate, 1g of sodium chloride, 1g of calcium chloride and 1000ml of water, wherein the pH value is adjusted to 7.0-7.2 by using sodium hydroxide or hydrochloric acid, and the fermentation conditions of the secondary pseudomonas brassicae propagation strain are as follows: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30-33 ℃, and the culture time is 24-36 hours;
the first-stage bacillus muralis fermentation medium comprises the following components: 3g of beef extract, 10g of peptone, 5g of sodium chloride and 1000ml of water, adjusting the pH value to 7.2-7.4 by using sodium hydroxide, and adjusting the fermentation conditions of first-stage bacillus muralis: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 24-48 hours;
the components of the fermentation medium of the second-level Geobacillus propagation strain are as follows: 5g of glucose, 3g of starch, 20g of soybean meal, 0.2g of yeast extract, 3g of dipotassium hydrogen phosphate, 1.5g of monopotassium phosphate, 0.006g of manganese sulfate, 0.5g of magnesium sulfate heptahydrate, 0.1g of ferric trichloride and 1000ml of water, wherein the pH value is adjusted to be 7.0-7.2 by using sodium hydroxide or hydrochloric acid, and the fermentation conditions of the bacillus muralis II are as follows: the ventilation rate is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 72-96 hours.
Preferably, the preparation method of the microbial fertilizer comprises the following steps:
A. stack fermentation
Mixing and stacking the livestock manure and the weathered coal uniformly to form a stack, controlling the temperature in the stack of the stack fermentation control strips to be 50-70 ℃, turning the stack every 2 days, and fermenting for 20-30 days;
B. mixed fermentation
And D, uniformly mixing the material obtained in the step A with the pseudomonas campestris fermentation liquor and the bacillus muralis fermentation liquor, and standing and fermenting for 7-10 days to obtain the special microbial fertilizer for the angelica.
Preferably, after the fermentation in the step A is finished, the method further comprises the step of air-drying the fermented material; the water content of the air-dried material is 10-15%; after the air drying treatment, the method also comprises the step of crushing the air-dried materials and sieving the crushed materials with a 80-mesh sieve.
Preferably, the livestock manure and the weathered coal are uniformly stacked in the step A to form a stack, wherein the stack is a strip stack with the width of 1.8-3 meters, the height of 1.2-1.5 meters and the length of more than or equal to 2 meters.
Preferably, the step B is performed with standing fermentation for 7-10 days at the temperature of 18-30 ℃.
The invention has the beneficial effects that:
1. the adding proportion of the organic fertilizer, the weathered coal and the organic carrier in the organic carrier fertilizer can ensure that the pH value of the fertilizer is between 6.9 and 7.2 and is close to the pH value of a secondary fermentation product of the organic carrier. The pH value of the whole production process tends to be stable, and the pH value is only finely adjusted as required in the secondary fermentation process of the organic carrier. The pH value is higher in organic matter content and viable count, and the two viable counts in the secondary fermentation are both higher than 2 hundred million. The product quality can easily reach the specification in the industrial standard NY/T798-2015 of the compound microbial fertilizer.
2. The advantages of mixing 50 parts of livestock manure and 50 parts of weathered coal by weight ratio and performing stack fermentation are that the nitrogen loss in the manure is less, the ammonia volatilization is low, and the environmental pollution is light.
3. The two-stage fermentation process is applied for fermentation, the fermentation time is short, the number of viable bacteria is high, and the spore yield is high. The 2 kinds of strain primary fermentation seed culture solutions adopt general beef extract peptone, the formula is simple, the universality is realized, the respective unique formulas are adopted for the secondary fermentation, the quantity of the produced viable bacteria of the pseudomonas rapae strain secondary fermentation culture solution is large, the bacterium solution has strong capability of resisting angelica root rot bacteria, the quantity of the produced viable bacteria of the bacillus muralis strain secondary fermentation culture solution is large, the spore conversion rate is high, and the spore conversion rate is more than 90%.
In conclusion, the disease-prevention angelica sinensis special microbial fertilizer and the preparation method thereof provided by the invention solve the problem of aggravation of soil-borne diseases caused by continuous cropping aiming at the problem that the variety of crops in an angelica sinensis genuine area is single and the diseases such as angelica root rot, scurf and the like commonly caused by continuous cropping planting are frequently generated in a large area in a staggered manner, improve the yield of angelica sinensis under the condition of reducing the using amount of pesticides and fertilizers, and promote growth and improve the disease resistance by adding two organic carriers;
the special microbial fertilizer for angelica sinensis produced by the invention has the following results in 3-year fertilizer effect comparison tests in Gansu Weiyuan, Min county, Longxi and other places: the microbial fertilizer produced by the technical scheme has obvious soil-borne disease resistance and yield increase effect when applied to angelica sinensis, has the control effect on the root rot of the angelica sinensis of 45-48 percent, and increases the yield by 12-16 percent.
Detailed Description
The special microbial fertilizer for the angelica comprises an organic fertilizer, weathered coal and an organic carrier, wherein the organic fertilizer is livestock manure, the organic carrier contains pseudomonas brassicae fermentation liquor and bacillus muralis fermentation liquor, and the special microbial fertilizer is prepared from the following components in parts by weight: 50-80 parts of livestock manure, 20-60 parts of weathered coal, 5-15 parts of pseudomonas brassicae fermentation liquor and 5-15 parts of bacillus muralis fermentation liquor.
The composition is preferably prepared from the following components in parts by weight: 50-60 parts of livestock manure, 30-50 parts of weathered coal, 6-12 parts of pseudomonas brassicae fermentation liquor and 6-12 parts of bacillus muralis fermentation liquor.
The composition is preferably prepared from the following components in parts by weight: 50 parts of livestock manure, 50 parts of weathered coal, 7.5 parts of pseudomonas brassicae fermentation liquor and 7.5 parts of bacillus muralis fermentation liquor.
The livestock manure is sheep manure, cow manure or a mixture of the sheep manure and the cow manure.
The pseudomonas brassicae fermentation liquor and the bacillus muralis fermentation liquor are subjected to two-stage fermentation, wherein
The components of the primary rape pseudomonas fermentation medium are as follows: 3g of beef extract, 10g of peptone, 5g of sodium chloride and 1000ml of water, adjusting the pH value to 7.2-7.4 by using sodium hydroxide, and adjusting the fermentation conditions of the first-grade pseudomonas brassicae: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 18-24 hours;
the components of the fermentation medium of the second-level pseudomonas brassicae propagation strain are as follows: 20g of glucose, 10g of peptone, 1g of dipotassium hydrogen phosphate, 1g of monopotassium phosphate, 0.5g of magnesium sulfate heptahydrate, 1g of sodium chloride, 1g of calcium chloride and 1000ml of water, wherein the pH value is adjusted to 7.0-7.2 by using sodium hydroxide or hydrochloric acid, and the fermentation conditions of the secondary pseudomonas brassicae propagation strain are as follows: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30-33 ℃, and the culture time is 24-36 hours;
the first-stage bacillus muralis fermentation medium comprises the following components: 3g of beef extract, 10g of peptone, 5g of sodium chloride and 1000ml of water, adjusting the pH value to 7.2-7.4 by using sodium hydroxide, and adjusting the fermentation conditions of first-stage bacillus muralis: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 24-48 hours;
the components of the fermentation medium of the second-level Geobacillus propagation strain are as follows: 5g of glucose, 3g of starch, 20g of soybean meal, 0.2g of yeast extract, 3g of dipotassium hydrogen phosphate, 1.5g of monopotassium phosphate, 0.006g of manganese sulfate, 0.5g of magnesium sulfate heptahydrate, 0.1g of ferric trichloride and 1000ml of water, wherein the pH value is adjusted to be 7.0-7.2 by using sodium hydroxide or hydrochloric acid, and the fermentation conditions of the bacillus muralis II are as follows: the ventilation rate is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 72-96 hours.
The preparation method of the microbial fertilizer special for angelica comprises the following steps:
A. stack fermentation
Uniformly stacking the livestock manure and weathered coal into a stack, wherein the width of the stack is 1.8-3 m, the height of the stack is 1.2-1.5 m, the length of the stack is more than or equal to 2 m, stacking and fermenting the stack at the temperature of 50-70 ℃ in the stack, turning the stack every 2 days, and fermenting for 20-30 days; air drying the fermented material to water content of 10-15%, pulverizing, and sieving with 80 mesh sieve.
B. Mixed fermentation
And D, uniformly mixing the material obtained in the step A with the pseudomonas campestris fermentation liquor and the bacillus muralis fermentation liquor, and standing and fermenting for 7-10 days at the temperature of 18-30 ℃ to obtain the special microbial fertilizer for the angelica.
The material sources used by the invention are as follows:
pseudomonas brassicae and bacillus paridis species: prepared by the method.
Livestock manure: and (4) purchasing in a sheep farm or a cattle farm.
Weathered coal: purchased from Tianwang fertilizer Co., Ltd, Ughai city, inner Mongolia.
Example 1:
the special microbial fertilizer for the angelica comprises an organic fertilizer, weathered coal and an organic carrier, wherein the organic fertilizer is livestock manure, the organic carrier contains pseudomonas brassicae fermentation liquor and bacillus muralis fermentation liquor, and the special microbial fertilizer is prepared from the following components in parts by weight: 50 parts of livestock manure, 20 parts of weathered coal, 5 parts of pseudomonas brassicae fermentation liquor and 5 parts of bacillus muralis fermentation liquor. The livestock manure is sheep manure, cow manure or a mixture of the sheep manure and the cow manure.
The pseudomonas brassicae fermentation liquor and the bacillus muralis fermentation liquor are subjected to two-stage fermentation, wherein
The components of the primary rape pseudomonas fermentation medium are as follows: 3g of beef extract, 10g of peptone, 5g of sodium chloride and 1000ml of water, adjusting the pH value to 7.2-7.4 by using sodium hydroxide, and adjusting the fermentation conditions of the first-grade pseudomonas brassicae: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 18-24 hours;
the components of the fermentation medium of the second-level pseudomonas brassicae propagation strain are as follows: 20g of glucose, 10g of peptone, 1g of dipotassium hydrogen phosphate, 1g of monopotassium phosphate, 0.5g of magnesium sulfate heptahydrate, 1g of sodium chloride, 1g of calcium chloride and 1000ml of water, wherein the pH value is adjusted to 7.0-7.2 by using sodium hydroxide or hydrochloric acid, and the fermentation conditions of the secondary pseudomonas brassicae propagation strain are as follows: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30-33 ℃, and the culture time is 24-36 hours;
the first-stage bacillus muralis fermentation medium comprises the following components: 3g of beef extract, 10g of peptone, 5g of sodium chloride and 1000ml of water, adjusting the pH value to 7.2-7.4 by using sodium hydroxide, and adjusting the fermentation conditions of first-stage bacillus muralis: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 24-48 hours;
the components of the fermentation medium of the second-level Geobacillus propagation strain are as follows: 5g of glucose, 3g of starch, 20g of soybean meal, 0.2g of yeast extract, 3g of dipotassium hydrogen phosphate, 1.5g of monopotassium phosphate, 0.006g of manganese sulfate, 0.5g of magnesium sulfate heptahydrate, 0.1g of ferric trichloride and 1000ml of water, wherein the pH value is adjusted to be 7.0-7.2 by using sodium hydroxide or hydrochloric acid, and the fermentation conditions of the bacillus muralis II are as follows: the ventilation rate is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 72-96 hours.
The preparation method of the microbial fertilizer special for angelica comprises the following steps:
A. stack fermentation
Mixing and stacking the livestock manure and the weathered coal uniformly into a stack, stacking strips with the width of 1.8 meters, the height of 1.2 meters and the length of 2 meters, controlling the temperature in the stack to be 50-70 ℃ by stacking and fermenting, and turning the stack every 2 days for 20 days; and (3) air-drying the fermented material until the water content is 10%, and crushing and sieving the material by a 80-mesh sieve.
B. Mixed fermentation
And D, uniformly mixing the material obtained in the step A with the pseudomonas campestris fermentation liquor and the bacillus muralis fermentation liquor, and standing and fermenting for 7 days at the temperature of 18-30 ℃ to obtain the special microbial fertilizer for the angelica.
Example 2:
the special microbial fertilizer for the angelica comprises an organic fertilizer, weathered coal and an organic carrier, wherein the organic fertilizer is livestock manure, the organic carrier contains pseudomonas brassicae fermentation liquor and bacillus muralis fermentation liquor, and the special microbial fertilizer is prepared from the following components in parts by weight: 80 parts of livestock manure, 60 parts of weathered coal, 15 parts of pseudomonas brassicae fermentation liquor and 15 parts of bacillus muralis fermentation liquor.
The livestock manure is sheep manure, cow manure or a mixture of the sheep manure and the cow manure.
The pseudomonas brassicae fermentation liquor and the bacillus muralis fermentation liquor are subjected to two-stage fermentation, wherein
The components of the primary rape pseudomonas fermentation medium are as follows: 3g of beef extract, 10g of peptone, 5g of sodium chloride and 1000ml of water, adjusting the pH value to 7.2-7.4 by using sodium hydroxide, and adjusting the fermentation conditions of the first-grade pseudomonas brassicae: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 18-24 hours;
the components of the fermentation medium of the second-level pseudomonas brassicae propagation strain are as follows: 20g of glucose, 10g of peptone, 1g of dipotassium hydrogen phosphate, 1g of monopotassium phosphate, 0.5g of magnesium sulfate heptahydrate, 1g of sodium chloride, 1g of calcium chloride and 1000ml of water, wherein the pH value is adjusted to 7.0-7.2 by using sodium hydroxide or hydrochloric acid, and the fermentation conditions of the secondary pseudomonas brassicae propagation strain are as follows: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30-33 ℃, and the culture time is 24-36 hours;
the first-stage bacillus muralis fermentation medium comprises the following components: 3g of beef extract, 10g of peptone, 5g of sodium chloride and 1000ml of water, adjusting the pH value to 7.2-7.4 by using sodium hydroxide, and adjusting the fermentation conditions of first-stage bacillus muralis: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 24-48 hours;
the components of the fermentation medium of the second-level Geobacillus propagation strain are as follows: 5g of glucose, 3g of starch, 20g of soybean meal, 0.2g of yeast extract, 3g of dipotassium hydrogen phosphate, 1.5g of monopotassium phosphate, 0.006g of manganese sulfate, 0.5g of magnesium sulfate heptahydrate, 0.1g of ferric trichloride and 1000ml of water, wherein the pH value is adjusted to be 7.0-7.2 by using sodium hydroxide or hydrochloric acid, and the fermentation conditions of the bacillus muralis II are as follows: the ventilation rate is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 72-96 hours.
The preparation method of the microbial fertilizer special for angelica comprises the following steps:
A. stack fermentation
Mixing and stacking the livestock manure and the weathered coal uniformly into a stack, stacking strips with the width of 3 meters, the height of 1.5 meters and the length of 100 meters, controlling the temperature in the stack to be 50-70 ℃ by stacking and fermenting, and turning the stack every 2 days for 30 days; and (3) air-drying the fermented material until the water content is 15%, crushing and sieving by a 80-mesh sieve.
B. Mixed fermentation
And D, uniformly mixing the material obtained in the step A with the pseudomonas campestris fermentation liquor and the bacillus muralis fermentation liquor, and standing and fermenting for 10 days at the temperature of 18-30 ℃ to obtain the special microbial fertilizer for the angelica.
Example 3:
the special microbial fertilizer for the angelica comprises an organic fertilizer, weathered coal and an organic carrier, wherein the organic fertilizer is livestock manure, and the organic carrier contains pseudomonas brassicae fermentation liquor and bacillus muralis fermentation liquor, and is prepared from the following components in parts by weight: 50 parts of livestock manure, 50 parts of weathered coal, 7.5 parts of pseudomonas brassicae fermentation liquor and 7.5 parts of bacillus muralis fermentation liquor.
The livestock manure is sheep manure, cow manure or a mixture of the sheep manure and the cow manure.
The pseudomonas brassicae fermentation liquor and the bacillus muralis fermentation liquor are subjected to two-stage fermentation, wherein
The components of the primary rape pseudomonas fermentation medium are as follows: 3g of beef extract, 10g of peptone, 5g of sodium chloride and 1000ml of water, adjusting the pH value to 7.2-7.4 by using sodium hydroxide, and adjusting the fermentation conditions of the first-grade pseudomonas brassicae: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 18-24 hours;
the components of the fermentation medium of the second-level pseudomonas brassicae propagation strain are as follows: 20g of glucose, 10g of peptone, 1g of dipotassium hydrogen phosphate, 1g of monopotassium phosphate, 0.5g of magnesium sulfate heptahydrate, 1g of sodium chloride, 1g of calcium chloride and 1000ml of water, wherein the pH value is adjusted to 7.0-7.2 by using sodium hydroxide or hydrochloric acid, and the fermentation conditions of the secondary pseudomonas brassicae propagation strain are as follows: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30-33 ℃, and the culture time is 24-36 hours;
the first-stage bacillus muralis fermentation medium comprises the following components: 3g of beef extract, 10g of peptone, 5g of sodium chloride and 1000ml of water, adjusting the pH value to 7.2-7.4 by using sodium hydroxide, and adjusting the fermentation conditions of first-stage bacillus muralis: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 24-48 hours;
the components of the fermentation medium of the second-level Geobacillus propagation strain are as follows: 5g of glucose, 3g of starch, 20g of soybean meal, 0.2g of yeast extract, 3g of dipotassium hydrogen phosphate, 1.5g of monopotassium phosphate, 0.006g of manganese sulfate, 0.5g of magnesium sulfate heptahydrate, 0.1g of ferric trichloride and 1000ml of water, wherein the pH value is adjusted to be 7.0-7.2 by using sodium hydroxide or hydrochloric acid, and the fermentation conditions of the bacillus muralis II are as follows: the ventilation rate is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 72-96 hours.
The preparation method of the microbial fertilizer special for angelica comprises the following steps:
A. stack fermentation
Mixing and stacking the livestock manure and the weathered coal uniformly into a stack, stacking strips with the width of 2 meters, the height of 1.5 meters and the length of 10 meters, controlling the temperature in the stack to be 50-70 ℃ by stacking and fermenting, and turning the stack every 2 days for 25 days; and (3) air-drying the fermented material until the water content is 10%, and crushing and sieving the material by a 80-mesh sieve.
B. Mixed fermentation
And D, uniformly mixing the material obtained in the step A with the pseudomonas campestris fermentation liquor and the bacillus muralis fermentation liquor, and standing and fermenting for 10 days at the temperature of 18-30 ℃ to obtain the special microbial fertilizer for the angelica.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The application of the microbial fertilizer in the soil-borne disease resistance and/or yield increase of the angelica sinensis is characterized in that the microbial fertilizer consists of an organic fertilizer, weathered coal and an organic carrier; the organic fertilizer is livestock manure; the organic carrier is a pseudomonas brassicae fermentation liquid and a bacillus muralis fermentation liquid.
2. The application of claim 1, wherein the microbial fertilizer is prepared from the following components in parts by weight: 50-80 parts of livestock manure, 20-60 parts of weathered coal, 5-15 parts of pseudomonas brassicae fermentation liquor and 5-15 parts of bacillus muralis fermentation liquor.
3. The application of claim 1, wherein the microbial fertilizer is prepared from the following components in parts by weight: 50-60 parts of livestock manure, 30-50 parts of weathered coal, 6-12 parts of pseudomonas brassicae fermentation liquor and 6-12 parts of bacillus muralis fermentation liquor.
4. The use according to claim 2 or 3, wherein the microbial fertilizer is formulated from the following parts by weight: 50 parts of livestock manure, 50 parts of weathered coal, 7.5 parts of pseudomonas brassicae fermentation liquor and 7.5 parts of bacillus muralis fermentation liquor.
5. Use according to claim 1, wherein the livestock manure is sheep manure and/or cattle manure.
6. The use according to claim 1, wherein the Pseudomonas brassicae fermentation broth, the Bacillus muralis fermentation broth are subjected to a two-stage fermentation, wherein,
the components of the primary rape pseudomonas fermentation medium are as follows: 3g of beef extract, 10g of peptone, 5g of sodium chloride and 1000ml of water, and adjusting the pH value to 7.2-7.4 by using sodium hydroxide; fermentation conditions of the pseudomonas brassicae: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 18-24 hours;
the components of the fermentation medium of the second-level pseudomonas brassicae propagation strain are as follows: 20g of glucose, 10g of peptone, 1g of dipotassium hydrogen phosphate, 1g of monopotassium phosphate, 0.5g of magnesium sulfate heptahydrate, 1g of sodium chloride, 1g of calcium chloride and 1000ml of water, wherein the pH value is adjusted to 7.0-7.2 by using sodium hydroxide or hydrochloric acid, and the fermentation conditions of the secondary pseudomonas brassicae propagation strain are as follows: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30-33 ℃, and the culture time is 24-36 hours;
the first-stage bacillus muralis fermentation medium comprises the following components: 3g of beef extract, 10g of peptone, 5g of sodium chloride and 1000ml of water, adjusting the pH value to 7.2-7.4 by using sodium hydroxide, and adjusting the fermentation conditions of first-stage bacillus muralis: the ventilation volume is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 24-48 hours;
the components of the fermentation medium of the second-level Geobacillus propagation strain are as follows: 5g of glucose, 3g of starch, 20g of soybean meal, 0.2g of yeast extract, 3g of dipotassium hydrogen phosphate, 1.5g of monopotassium phosphate, 0.006g of manganese sulfate, 0.5g of magnesium sulfate heptahydrate, 0.1g of ferric trichloride and 1000ml of water, wherein the pH value is adjusted to be 7.0-7.2 by using sodium hydroxide or hydrochloric acid, and the fermentation conditions of the bacillus muralis II are as follows: the ventilation rate is 4.5NL/min, the stirring speed is 300r/min, the culture temperature is 30 ℃, and the culture time is 72-96 hours.
7. The use according to claim 1, characterized in that the preparation process of the microbial fertilizer comprises the following steps:
A. stack fermentation
Mixing and stacking the livestock manure and the weathered coal uniformly to form a stack, controlling the temperature in the stack of the stack fermentation control strips to be 50-70 ℃, turning the stack every 2 days, and fermenting for 20-30 days;
B. mixed fermentation
And D, uniformly mixing the material obtained in the step A with the pseudomonas campestris fermentation liquor and the bacillus muralis fermentation liquor, and standing and fermenting for 7-10 days to obtain the special microbial fertilizer for the angelica.
8. The application of claim 7, wherein the step A further comprises air drying the fermented material after the fermentation is finished; the water content of the air-dried material is 10-15%; after the air drying treatment, the method also comprises the step of crushing the air-dried materials and sieving the crushed materials with a 80-mesh sieve.
9. The use of claim 7, wherein step A comprises stacking the animal manure and weathered coal in a stack of strips having a width of 1.8-3 m, a height of 1.2-1.5 m, and a length of 2 m or more.
10. The use of claim 7, wherein the fermentation in step B is carried out at 18-30 ℃ for 7-10 days.
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| CN201610585180.3A CN106187345A (en) | 2016-07-25 | 2016-07-25 | A kind of Radix Angelicae Sinensis microbial fertilizer special and preparation method thereof |
| CN202111261353.3A CN113880616A (en) | 2016-07-25 | 2016-07-25 | Application of microbial fertilizer in soil-borne disease resistance and/or yield increase of angelica sinensis |
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| CN108821835A (en) * | 2018-07-20 | 2018-11-16 | 贵州珍安药业有限公司 | A kind of fertilizer special for Chinese angelica and its production method |
| CN113402327B (en) * | 2021-07-28 | 2023-02-03 | 甘肃省农业科学院植物保护研究所 | Special microbial compound fertilizer for vegetables and preparation method thereof |
| CN114181017A (en) * | 2021-12-15 | 2022-03-15 | 北京嘉博文生物科技有限公司 | Composition for relieving angelica sinensis continuous cropping obstacle and preparation method and application thereof |
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