CN101671205B - Composite microbial preparation for promoting growth of rape and preparation method thereof - Google Patents
Composite microbial preparation for promoting growth of rape and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a composite microbial preparation for promoting the growth of rape and a preparation method thereof. The composite microbial preparation for promoting the growth of the rape is characterized by comprising 20-40 parts by weight of strain culturing substance and 60-80 parts by weight of microbial carrier, wherein the strain culturing substance comprises the following raw materials in parts by weight: 2-8 parts of bacillus subtilis, 2-8 parts of bacillus megaterium, 5-10 parts of rhodopseudomonas palustris and 1-4 parts of azotobacter chroococcum. The preparation method of the composite microbial preparation for promoting the growth of the rape comprises the following steps: culturing 2-8 parts of bacillus subtilis, 2-8 parts of bacillus megaterium and the rhodopseudomonas palustris together, culturing the azotobacter chroococcum independently, mixing and fermenting. The invention has the advantages of promoting the growth and the propagation of beneficial microorganisms, increasing the absorption of the root system of the rape to nitrogen, phosphorus, potassium and other organic substances and promoting the normal development of the root system, thus promoting the growth of the rape and reducing the applied chemical fertilizer.
Description
Technical field
The present invention relates to a kind of complex microorganism preparations that promotes growth of rape and preparation method thereof, be applied in root system of plant in the soil, the nitrogen that is used for fixed air is organonitrogen or replenishes nitrogen in the soil by salt, belongs to the fertilizer field.
Background technology
Rape is about 8,000,000 hectares in the long-term cultivated area of China, and its seed oil content is 30%-50%, is the important oil crops of China.For a long time, China's rapeseed cultivation area and ultimate production all rank first in the world, and account for about 30% of world's rapeseed cultivation area and ultimate production.After the accession to WTO, the various markets for farm products of China all are faced with and are in for a long time protected status, lack the strong market competitiveness.Along with internationalization the further developing of market, can the rape industry of China and market spend this period stably is a challenge in addition.
China most of rape producing region, output all is lower than 200 kilograms every mu, is a bottleneck that restricts for a long time ultimate production.In recent years, owing to many factors such as planting benefit descend, the transformation of scientific and technical result is slow, market value is low, the significantly situation of minimizing appears in rapeseed area.Current, soaring, the exploitation of biofuel of world's Semen Brassicae campestris price, the supply and demand in market are not being met, for the rape development has brought opportunity.
From between 1950-1990 40 years, fertilizer production is used in the whole world has increased by 27 times unexpectedly, reaches 800,000,000 tons, and the at present stagnation of yield of rape and decline is because the incomplete absorption of nitrogen causes, and only has the amount of application that increases nitrogenous fertilizer just can keep or improve output.The environmental pollution that causes for the consumption that reduces Nonrenewable resources and fertilising; realize the sustainable development of agricultural; the protection eubiosis; people are just attempting to reduce fertilizer amount; hope addresses the above problem by some novel bio-feritlizers and biological nitrogen fixation, improves the particularly output of rape of crop.Since German microbiologist Lorenz Hiltner in 1904 has proposed " root circle " (rhizosphere) since this concept, about many-sided knowledge such as its biology, microbiology and ultrastructure has been studied by people and deep understanding arranged.The rhizosphere effect that in early days research of root circle mainly is limited to microorganism, after the sixties because the application of electron microscope in soil science and microbiological research, tentative confirmation exist the complex relationship of root system of plant, rhizosphere microorganism and edatope three interaction in the root circle micro-ecological environment.
Existing photosynthetic microorganism fertilizer generally all is single culture, and the vigor of bacterium is generally 10
6-10
8The cfu/ gram, function is also more single, and crop and microorganism are loose associating, do not form the weave construction of symbiosis between them, so the activity of fixed nitrogen are subjected to many Restricted requirements easily.
Summary of the invention
The purpose of this invention is to provide a kind of micro-ecological environment that can effectively improve crop root, promote the beneficial microorganism growth and breeding, promote simultaneously rape root to the absorption of nitrogen, phosphorus, potassium and other organic matters, promote the normal development of root system, use chemical fertilizer thereby promote growth of rape to reduce, reach a kind of complex microorganism preparations and preparation method of safe, green planting effect.
In order to realize above purpose, technical scheme of the present invention provides a kind of preparation method who promotes the complex microorganism preparations of growth of rape, it is characterized in that, concrete steps are:
The first step: with the first liquid substratum 115-130 ℃ of sterilization after 15-30 minute with tween with weight ratio 100: 0.3-0.5 mixes, with subtilis (Bacillus subtilis) ACCC 11060, the mixture of bacillus megaterium (Bacillus meyaterium) ACCC 10010 and Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC 10649 is by weight 1: the ratio of 50-100 is inoculated in the above-mentioned first liquid substratum mixture, 25-35 ℃ stir 15-25 minute with 150-300 rev/min speed after airtight standing for fermentation 3-5 days, sampling Detection thalli growth situation and measure pH value stops to ferment when 1,000,000,000/ml and pH value reach 3.0-4.0 when microscopically viable bacteria total concn number surpasses; Described first liquid substratum is comprised of following raw material in weight part: bean cake powder 3-5, soyflour 2-4, Semen Maydis powder 1-2, bicarbonate of ammonia 0.01-0.3, molasses 2-10, potassium primary phosphate 1.2-2, sal epsom 0.01-0.8, manganous sulfate 0.001-1, Sodium Propionate 0.1-0.5, ammonium chloride 0.05-0.5 and sterilized water 75.8-90.8;
Second step: the second liquid substratum was sterilized 15-30 minute at 115-130 ℃, first ventilation, ventilation volume was than 1: 20, again with blown-ball Azotobacter (Azotobacter chroococcum) ACCC 10007 by weight 1: 50-100 is inoculated in the second liquid substratum, standing for fermentation after the 25-35 ℃ of speed with 150-300 rev/min stirs 15-25 minute, continue ventilation in the fermenting process, the ventilation volume ratio is 1: 20, sampling Detection thalli growth situation, when microscopically viable bacteria total concn number surpasses 1,000,000,000/ml, stop fermentation; Described second liquid substratum is comprised of following raw material in weight part: N.F,USP MANNITOL 10-20, bean cake powder 2-10, molasses 2-10, Semen Maydis powder 2-10, potassium primary phosphate 0.5-1, SODIUM PHOSPHATE, MONOBASIC 1-2, sal epsom 0.3-1 and sterilized water 46-82.2;
The 3rd step: respectively with the microbial strain culture of the first step and second step gained respectively with the centrifugal 5-25 of 2000-4000 rev/min rotating speed minute, and at 42-45 ℃ of dry 10-12 hour water content is reduced to below the 30wt%, microbial strain culture is mixed in proportion, with microbial strain culture mixture and microbe carrier by weight 20-40: 60-80 mixes, broken in room temperature, cross the 60-100 mesh sieve;
The weight ratio of blown-ball Azotobacter (Azotobacter chroococcum) ACCC 10007 that used subtilis (Bacillus subtilis) ACCC 11060, bacillus megaterium (Bacillus meyaterium) ACCC 10010, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC 10649 and the second step of the first step is used is 2-8: 2-8: 5-10: 1-4;
The 3rd step, described microbe carrier was plant ash, wilkinite or their mixture.
Four kinds of bacterial classifications that adopt among the present invention are to buy from agriculture microbial strains preservation administrative center, and the information of each bacterial classification is as follows: subtilis (Bacillus subtilis) ACCC 11060; Bacillus megaterium (Bacillus meyaterium) ACCC 10010; Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC 10649; Blown-ball Azotobacter (Azotobacter chroococcum) ACCC 10007.
Principle of the present invention is as follows: the present invention is by the multiple beneficial the composition of the microorganism, utilizes their phosphorus decomposing, potassium, degraded toxic substance, improves crop anti-adversity, promotes the multiple efficacies such as soil ulmin formation.The carrier that also has simultaneously special adsorption function, can be in the medium-term and long-term survival of soil, can effectively improve the micro-ecological environment of crop root, promote the beneficial microorganism growth and breeding, suppress simultaneously and kill and wound to be harmful to humic bacterium or disease and pest, promote the normal development of root system, thereby promote growth of rape to reduce or stop to use chemical fertilizer, reach a kind of safe, green planting effect, adopt the probiotics of normal growth in soil in the product, quantity is repaired modifying agent up to 1,000,000,000/g and the planting soil microbial ecological that contains the various active metabolic substd.After use, can form benign eco-environment in the crop soil, promote crop that the absorption of nutrient is transformed, improve Soil structure, suppress the Growth and reproduction of harmful pathogenic bacteria, decompose the remaining agricultural chemicals in soil and the crop.
They can discharge potassium and the phosphorus of the invalid attitude of soil mineral for growth and development of plants to contain a lot of potassium bacteriums and phosphorus bacteria in the soil, the a lot of at present phosphate-solubilizing bacterias of report of the microbe species of phosphorus decomposing mainly contain bacillus (Bacillus) Rhodopseudomonas (Pseudomonas) rhizobium (Rhizobium) etc., roots of plants circle beneficial bacteria is plant-growth promoting rhizobacteria (Plant-Growth-promoting Rhizobacteria, be called for short PGPR), it is ubiquitous at the roots of plants circle, the multiple different bacterium that is active in the roots of plants circle, sick by pressing down, directly stimulating plant growth, increase can utilize nutrition, the Promoting plant growths such as the dross of enhancing root nodule bacterium plant root and generation induction of resistance, increase crop yield.The present invention is also also fermented subtilis, bacillus megaterium, Rhodopseudomonas palustris, and its quantity can be up to 10
9Individual/g.
Although above-mentioned different bacterium and fungi can play the part of a plurality of effects such as " phosphorus decomposings; potassium; degraded toxic substance; improve crop anti-adversity; promote soil ulmin " in planting soil, but microorganism is a kind of simple prokaryotic organism after all, its growth, pH value in the edatope is received in breeding, moisture, temperature, air content, impact Deng optimal growth conditions, the speed of growth of harmful pathogenic bacteria such as putrefactive bacterium is faster than them simultaneously, so growing environment or the carrier of a safety are provided, it is the prerequisite of these beneficial microorganism effects of performance, some simple inorganicss both can be used as soil improvement agent and had used in soil in fact, can be used as again the good carrier of beneficial microorganism, the carrier among the present invention is by plant ash, calcium carbonate forms.This class material has stronger adsorptive power, the grogs that disperses can be attached together, and forms soil agreegate, plays the effect of water conservation, moisturizing.
Advantage of the present invention is:
1. adopt and to promote the crop root growth and improve edatope multiple beneficial bacterium and fungi, reasonable compatibility, to promote crop root to grow, the effects such as phosphorus decomposing, potassium, degraded toxic substance, raising crop anti-adversity, the formation of promotion soil ulmin organically combine;
2. the extracellular enzyme that also has probiotics to produce in liquid culture such as proteolytic enzyme, cellulase etc., can decompose rapidly front trouble straw, increases operation rate, and reduces fertilizer application;
3. adopting food crop and byproduct commonly used is main medium, and cost is low, product safety is reliable.
Embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1
A kind of complex microorganism preparations that promotes growth of rape is comprised of microbial strain culture 20 weight parts and microbe carrier 80 weight parts; Wherein, described microbial strain culture is comprised of following raw material in weight part: 1 part of 2 parts of subtilises, 2 parts of bacillus megateriums, 5 parts of Rhodopseudomonas palustriss and blown-ball Azotobacter, and above bacterial classification all can be bought in Chinese agriculture microbial strains preservation administrative center; Described microbe carrier is plant ash.
The preparation method's of the complex microorganism preparations of above-mentioned promotion growth of rape concrete steps are:
The first step: the first liquid substratum is mixed with weight ratio with tween-80 after 15 minutes 115 ℃ of sterilizations at 100: 0.3, the mixture of subtilis, bacillus megaterium and the Rhodopseudomonas palustris ratio by weight 1: 50 is inoculated in the above-mentioned first liquid substratum mixture, the plastic tank standing for fermentation is 3 days put into sterilization after 25 ℃ of speed with 150 rev/mins stir 15 minutes after, sampling Detection thalli growth situation and measure pH value stops to ferment when 1,000,000,000/ml and pH value reach 3.0 when microscopically viable bacteria total concn number surpasses;
Described first liquid substratum is comprised of following raw material in weight part: bean cake powder 3, soyflour 2, Semen Maydis powder 1, molasses 2, potassium primary phosphate 1.2, manganous sulfate 0.001, Sodium Propionate 0.2, ammonium chloride 0.05 and sterilized water 90.6.
Second step: the second liquid substratum was sterilized 15 minutes at 115 ℃, first ventilation, ventilation ratio 1: 20 (volume ratio) was inoculated in blown-ball Azotobacter in the second liquid substratum by weight 1: 50 again, standing for fermentation after 25 ℃ of speed with 150 rev/mins stir 15 minutes, continue ventilation in the fermenting process, ventilation ratio is 1: 20 (volume ratio), sampling Detection thalli growth situation, when microscopically viable bacteria total concn number surpasses 1,000,000,000/ml, stop fermentation;
Described second liquid substratum is comprised of following raw material in weight part: N.F,USP MANNITOL 10, bean cake powder 2, molasses 2, Semen Maydis powder 10, potassium primary phosphate 1, SODIUM PHOSPHATE, MONOBASIC 2, sal epsom 1 and sterilized water 82.2.
The 3rd step: respectively with the microbial strain culture of the first step and second step gained with 2000 rev/mins rotating speed centrifugal 5 minutes, and 42 ℃ of dryings water content is reduced to below the 30wt%, microbial strain culture is mixed in proportion, microbial strain culture mixture and microbe carrier are mixed in proportion, in 25 ℃ of fragmentations of room temperature, cross 60 mesh sieves, the product after sieving is incorporated with in the packing bag of individual event purging valve, preserve at dry, lucifuge place.
Embodiment 2
A kind of complex microorganism preparations that promotes growth of rape is comprised of microbial strain culture 40 weight parts and microbe carrier 60 weight parts; Wherein, described microbial strain culture is comprised of following raw material in weight part: 4 parts of 8 parts of subtilises, 8 parts of bacillus megateriums, 10 parts of Rhodopseudomonas palustriss and blown-ball Azotobacters, and above bacterial classification all can be bought in Chinese agriculture microbial strains preservation administrative center; Described microbe carrier is wilkinite.
The preparation method's of the complex microorganism preparations of above-mentioned promotion growth of rape concrete steps are:
The first step: the first liquid substratum is mixed with weight ratio with tween-80 after 30 minutes 130 ℃ of sterilizations at 100: 0.5, the mixture of subtilis, bacillus megaterium and the Rhodopseudomonas palustris ratio by weight 1: 100 is inoculated in the above-mentioned first liquid substratum mixture, the plastic tank standing for fermentation is 5 days put into sterilization after 35 ℃ of speed with 300 rev/mins stir 25 minutes after, sampling Detection thalli growth situation and measure pH value stops to ferment when 1,000,000,000/ml and pH value reach 4.0 when microscopically viable bacteria total concn number surpasses;
Described first liquid substratum is comprised of following raw material in weight part: bean cake powder 5, soyflour 4, Semen Maydis powder 2, bicarbonate of ammonia 0.3, molasses 10, potassium primary phosphate 2, sal epsom 0.8, manganous sulfate 1, Sodium Propionate 0.2, ammonium chloride 0.1 and sterilized water 73.6.
Second step: the second liquid substratum was sterilized 30 minutes at 130 ℃, first ventilation, ventilation ratio 1: 20 (volume ratio) was inoculated in blown-ball Azotobacter in the second liquid substratum by weight 1: 100 again, standing for fermentation after 35 ℃ of speed with 300 rev/mins stir 25 minutes, continue ventilation in the fermenting process, ventilation ratio is 1: 20, sampling Detection thalli growth situation, when microscopically viable bacteria total concn number surpasses 1,000,000,000/ml, stop fermentation;
Described second liquid substratum is comprised of following raw material in weight part: N.F,USP MANNITOL 20, bean cake powder 10, molasses 10, Semen Maydis powder 10, potassium primary phosphate 1, SODIUM PHOSPHATE, MONOBASIC 2, sal epsom 1 and sterilized water 46.
The 3rd step: respectively with the microbial strain culture of the first step and second step gained with 4000 rev/mins rotating speed centrifugal 25 minutes, and 45 ℃ of dryings water content is reduced to below the 30wt%, microbial strain culture is mixed in proportion, microbial strain culture mixture and microbe carrier are mixed in proportion, 45 ℃ of fragmentations, cross 100 mesh sieves, the product after sieving is incorporated with in the packing bag of individual event purging valve, preserve at dry, lucifuge place.
Embodiment 3
A kind of complex microorganism preparations that promotes growth of rape is comprised of microbial strain culture 40 weight parts and microbe carrier 60 weight parts; Wherein, described microbial strain culture is comprised of following raw material in weight part: 3 parts of 6 parts of subtilises, 6 parts of bacillus megateriums, 6 parts of Rhodopseudomonas palustriss and blown-ball Azotobacters, and above bacterial classification all can be bought in Chinese agriculture microbial strains preservation administrative center; Described microbe carrier is that weight ratio is 2: 8 plant ash and bentonitic mixture.
The preparation method's of the complex microorganism preparations of above-mentioned promotion growth of rape concrete steps are:
The first step: the first liquid substratum is mixed with weight ratio with tween-80 after 20 minutes 125 ℃ of sterilizations at 100: 0.3, the mixture of subtilis, bacillus megaterium and the Rhodopseudomonas palustris ratio by weight 1: 100 is inoculated in the above-mentioned first liquid substratum mixture, the plastic tank standing for fermentation is 5 days put into sterilization after 35 ℃ of speed with 220 rev/mins stir 20 minutes after, sampling Detection thalli growth situation and measure pH value stops to ferment when 1,000,000,000/ml and pH value reach 4.0 when microscopically viable bacteria total concn number surpasses;
Described first liquid substratum is comprised of following raw material in weight part: bean cake powder 3, soyflour 2, Semen Maydis powder 1, molasses 10, potassium primary phosphate 1.2, manganous sulfate 0.001, Sodium Propionate 0.2, ammonium chloride 0.1 and sterilized water 79.5.
Second step: the second liquid substratum was sterilized 30 minutes at 130 ℃, blown-ball Azotobacter was inoculated in the second liquid substratum by weight 1: 50, standing for fermentation after 30 ℃ of speed with 230 rev/mins stir 20 minutes, continue ventilation in the fermenting process, ventilation ratio is 1: 20 (volume ratio), sampling Detection thalli growth situation when microscopically viable bacteria total concn number surpasses 1,000,000,000/ml, stops fermentation;
Described second liquid substratum is comprised of following raw material in weight part: N.F,USP MANNITOL 10, bean cake powder 5, molasses 5, Semen Maydis powder 2, potassium primary phosphate 0.5, SODIUM PHOSPHATE, MONOBASIC 1, sal epsom 0.5, sterilized water 76.
The 3rd step: respectively with the microbial strain culture of the first step and second step gained with 3000 rev/mins rotating speed centrifugal 25 minutes, and 45 ℃ of dryings water content is reduced to below the 25wt%, microbial strain culture is mixed in proportion, microbial strain culture mixture and microbe carrier are mixed in proportion, 45 ℃ of fragmentations, cross 80 mesh sieves, the product after sieving is incorporated with in the packing bag of individual event purging valve, preserve at dry, lucifuge place.
Embodiment 4
A kind of complex microorganism preparations that promotes growth of rape is comprised of microbial strain culture 40 weight parts and microbe carrier 60 weight parts; Wherein, described microbial strain culture is comprised of following raw material in weight part: 3 parts of 5 parts of subtilises, 10 parts of bacillus megateriums, 6 parts of Rhodopseudomonas palustriss and blown-ball Azotobacters, and above bacterial classification all can be bought in Chinese agriculture microbial strains preservation administrative center; Described microbe carrier is that weight ratio is 1: 9 plant ash and bentonitic mixture.
The preparation method's of the complex microorganism preparations of above-mentioned promotion growth of rape concrete steps are:
The first step: the first liquid substratum is mixed with weight ratio with tween-80 after 20 minutes 130 ℃ of sterilizations at 100: 0.5, the mixture of subtilis, bacillus megaterium and the Rhodopseudomonas palustris ratio by weight 1: 50 is inoculated in the above-mentioned first liquid substratum mixture, the plastic tank standing for fermentation is 5 days put into sterilization after 35 ℃ of speed with 200 rev/mins stir 20 minutes after, sampling Detection thalli growth situation and measure pH value stops to ferment when 1,000,000,000/ml and pH value reach 4.0 when microscopically viable bacteria total concn number surpasses;
Described first liquid substratum is comprised of following raw material in weight part: bean cake powder 5, soyflour 4, Semen Maydis powder 2, bicarbonate of ammonia 0.3, molasses 14, potassium primary phosphate 2, sal epsom 0.8, manganous sulfate 0.002, Sodium Propionate 0.2, ammonium chloride 0.1 sterilized water 72.6.
Second step: the second liquid substratum was sterilized 25 minutes at 130 ℃, blown-ball Azotobacter was inoculated in the second liquid substratum by weight 1: 100, standing for fermentation after 30 ℃ of speed with 230 rev/mins stir 20 minutes, continue ventilation in the fermenting process, ventilation ratio is 1: 20, sampling Detection thalli growth situation when microscopically viable bacteria total concn number surpasses 1,000,000,000/ml, stops fermentation;
Described second liquid substratum is comprised of following raw material in weight part: N.F,USP MANNITOL 8, bean cake powder 5, molasses 7, Semen Maydis powder 2, potassium primary phosphate 0.5, SODIUM PHOSPHATE, MONOBASIC 1, sal epsom 0.5, sterilized water 76.
The 3rd step: respectively with the microbial strain culture of the first step and second step gained with 3000 rev/mins rotating speed centrifugal 25 minutes, and 45 ℃ of dryings water content is reduced to below the 25wt%, microbial strain culture is mixed in proportion, microbial strain culture mixture and microbe carrier are mixed in proportion, 45 ℃ of fragmentations, cross 80 mesh sieves, the product after sieving is incorporated with in the packing bag of individual event purging valve, preserve at dry, lucifuge place.
In use, according to different soil, product can use separately or mix rear use with fertilizer, before sowing or the transplanting, amount by about five grams of every strain plant evenly places bar ditch or hole bottom with this product, on cover and loosen the soil about five centimeters, can sow or transplant, the more serious plot of front stubble disease and pest can be taken the circumstances into consideration dosage and be used.When using base manure, also this product evenly can be sneaked into organic fertilizer in 2 liters every mu ratio and use.This product need be deposited in the hermetically drying place, avoids mixing stacking with agricultural chemicals, strong stimulation article etc., uses as early as possible behind the Kaifeng.
This product Agricultural Park that advances in Songjiang, Shanghai after research and development are finished carries out the rape simultaneous test, disposable addition is 500 g/acres, yield of rape improves 8-15% behind this biological soil Fertilizer application, root length on average increases 2-5cm, crop pest reduces 40-45%, ripe 5-10 days in advance of rape.
Claims (1)
1. a preparation method who promotes the complex microorganism preparations of growth of rape is characterized in that, concrete steps are:
The first step: with the first liquid substratum 115-130 ℃ of sterilization after 15-30 minute with tween with weight ratio 100: 0.3-0.5 mixes, with subtilis (Bacillus subtilis) ACCC 11060, the mixture of bacillus megaterium (Bacillus meyaterium) ACCC 10010 and Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC 10649 is by weight 1: the ratio of 50-100 is inoculated in the above-mentioned first liquid substratum mixture, 25-35 ℃ stir 15-25 minute with 150-300 rev/min speed after airtight standing for fermentation 3-5 days, sampling Detection thalli growth situation and measure pH value stops to ferment when 1,000,000,000/ml and pH value reach 3.0-4.0 when microscopically viable bacteria total concn number surpasses; Described first liquid substratum is comprised of following raw material in weight part: bean cake powder 3-5, soyflour 2-4, Semen Maydis powder 1-2, bicarbonate of ammonia 0.01-0.3, molasses 2-10, potassium primary phosphate 1.2-2, sal epsom 0.01-0.8, manganous sulfate 0.001-1, Sodium Propionate 0.1-0.5, ammonium chloride 0.05-0.5 and sterilized water 75.8-90.8;
Second step: the second liquid substratum was sterilized 15-30 minute at 115-130 ℃, first ventilation, ventilation volume was than 1: 20, again with blown-ball Azotobacter (Azotobacter chroococcum) ACCC 10007 by weight 1: 50-100 is inoculated in the second liquid substratum, standing for fermentation after the 25-35 ℃ of speed with 150-300 rev/min stirs 15-25 minute, continue ventilation in the fermenting process, the ventilation volume ratio is 1: 20, sampling Detection thalli growth situation, when microscopically viable bacteria total concn number surpasses 1,000,000,000/ml, stop fermentation; Described second liquid substratum is comprised of following raw material in weight part: N.F,USP MANNITOL 10-20, bean cake powder 2-10, molasses 2-10, Semen Maydis powder 2-10, potassium primary phosphate 0.5-1, SODIUM PHOSPHATE, MONOBASIC 1-2, sal epsom 0.3-1 and sterilized water 46-82.2;
The 3rd step: respectively with the microbial strain culture of the first step and second step gained respectively with the centrifugal 5-25 of 2000-4000 rev/min rotating speed minute, and at 42-45 ℃ of dry 10-12 hour water content is reduced to below the 30wt%, microbial strain culture is mixed in proportion, the microbial strain culture mixture is mixed for 20-40: 60-80 by weight with microbe carrier, broken in room temperature, cross the 60-100 mesh sieve;
The weight ratio of blown-ball Azotobacter (Azotobacter chroococcum) ACCC 10007 that used subtilis (Bacillus subtilis) ACCC 11060, bacillus megaterium (Bacillus meyaterium) ACCC 10010, Rhodopseudomonas palustris (Rhodopseudomonas palustris) ACCC 10649 and the second step of the first step is used is 2-8: 2-8: 5-10: 1-4;
The 3rd step, described microbe carrier was plant ash, wilkinite or their mixture.
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1475560A (en) * | 2003-07-14 | 2004-02-18 | 刘佳鸣 | Composite biological fungus agent |
| CN1583676A (en) * | 2003-08-21 | 2005-02-23 | 广东省龙眼洞林场 | Manufacturing method for microbial manure for subtropical forest sprouts |
| CN1587372A (en) * | 2004-09-08 | 2005-03-02 | 滨洲绿然生物技术开发有限公司 | Farm composite bacteria prepn and preparing method |
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| CN101671205A (en) | 2010-03-17 |
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