CN107129327A - Microbial manure and preparation method thereof - Google Patents

Microbial manure and preparation method thereof Download PDF

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Publication number
CN107129327A
CN107129327A CN201710249955.4A CN201710249955A CN107129327A CN 107129327 A CN107129327 A CN 107129327A CN 201710249955 A CN201710249955 A CN 201710249955A CN 107129327 A CN107129327 A CN 107129327A
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China
Prior art keywords
trichoderma
microbial manure
solid
preparation
culture
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Inventor
白志辉
姚东
焦海华
吕鹏翼
张换彩
赵品胜
张邦杰
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Hebei Fenghe Green Light Agricultural Science And Technology Co Ltd
Yueyang Green Light Agricultural Science And Technology Co Ltd
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Hebei Fenghe Green Light Agricultural Science And Technology Co Ltd
Yueyang Green Light Agricultural Science And Technology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

It is 5.0~6.0 the invention discloses the pH of a kind of microbial manure and preparation method thereof, including regulation cassava alcohol waste water, sterilizing obtains trichoderma liquid seed culture medium;The trichoderma viride of slant preservation is inoculated with to trichoderma liquid seed culture medium, trichoderma liquid seed is obtained after culture;Trichoderma liquid seed is inoculated with into cassava alcohol waste water, blowing air culture obtains trichoderma bacterium solution;Using the mixture of feces of livestock and poultry, bacteria residue and the stalk of crushing as solid culture based raw material, trichoderma bacterium solution is added in solid culture based raw material, static state is banked up, blowing air culture obtains product by solid-state fermentation;Processing is dried to product by solid-state fermentation, microbial manure is obtained.The present invention is not only able to reduce production cost in terms of raw materials for production and production process two, while high-concentration waste water, the disposal of pollutants of feces of livestock and poultry the like waste can also be reduced, improves salt affected soil improved performance.

Description

Microbial manure and preparation method thereof
Technical field
The present invention relates to technical field of biological fertilizer, and in particular to and a kind of cassava alcohol waste water and biomass solid waste liquid- Solid-state combined ferment production salt affected soil improvement microbial manure and preparation method thereof.
Background technology
The 1/2 of China's per capita area of cultivated farmland deficiency world average level, and middle-and-low-yielding fields account for 2/3.Chinese salt-soda soil distribution The salt-soda soil gross area in 17 provinces and regions including northwest, northeast, North China and coastal region, saline-alkali wasteland and influence arable land exceedes 500000000 mu, wherein with agricultural use potentiality up to 200,000,000 mu.Saline alkali land resource is developed, to alleviating after soil pressure, increase Emergency field deposit, Ensuring Food Safety, improve the ecological environment etc. it is significant.
Contain substantial amounts of beneficial microbe and organic matter in microbial manure, organic matter produces substantial amounts of in decomposable process Organic acid, can improve the physicochemical property of salinized soil and improve microbial activity, soil can be improved using microbial manure Earth physicochemical property (such as the reduction soil weight, increase soil porosity), reduces moisture evaporation, suppresses salt accumulation in soil;Improve soil Microecosystem, strengthens the fertility of soil;Improvement salinized soil is played a positive role.
Chinese invention patent (application number 201110203021.X) discloses a kind of soil improvement of improvement soil salt alkalescence Agent, its preparation method be by by Rhodopseudomonas palustris, Pichia membranaefaciens bacterium, Lactobacillus casei, streptomyces microflavus with sea Algae slurry is mixed, and organically combines multiple beneficial microbial bacteria and marine alga through repeatedly fermentation, new micro- life of compound criteria Thing live bacteria agent-seaweed bio-fungus, and humic acid, organic matter, activated zeolite powder and the molasses of appropriate number are added, then it is fermented Tunning is dried afterwards.Chinese invention patent (application number 200810017565.5) discloses a kind of biological water-retention saline alkali soil Modifying agent, is by plant straw powder, humic acid, water-loss reducer, urea, gypsum, high-activity biological preparation, sodium ion adsorbent, acid Mixed with certain proportion, then add binding agent, granulation is obtained.Chinese invention patent (application number 201210286131.1) is open A kind of saline-alkali soil conditioner, is by fish aquatic products processing discarded object powder, oyster shell whiting, powder of straw, diatomite, high-activity biological Preparation, acid, medical stone powder mix gained with certain proportion.Chinese invention patent (application number 201510393131.5) discloses one The microbial organic fertilizer with alkaline land improving effect is planted, the step of complex microorganism two is passed through using marine alga, stalk, excrement as raw material It is prepared by zymotechnique.Chinese invention patent (application number 201510567766.2) discloses a kind of bio-fertilizer in improvement salt-soda soil Material, its component includes:Ferment mushroom residue, ammonium sulfate, calcium superphosphate, composite modifying agent, EDTA- metallo-chelates, ammonium molybdate, boron Sand and complex micro organism fungicide.Chinese invention patent (application number 201610599170.5) discloses a kind of full nutrition salt-soda soil soil Earth modifying agent, it contains microorganism species, fertilizer, plant growth regulator, insecticide and rare earth silicon-calcium mineral matter, various raw materials Uniform mixed dissolution.Chinese invention patent (application number 201610523918.3) discloses a kind of salt-soda soil improvement fertilizer And preparation method thereof, its raw material includes:Maleic anhydride, citric acid, calcium superphosphate, sodium ion adsorbent, microbial bacterial agent, yellow corruption Acid, trace-element fertilizer, chicken manure fermenting fertilizer, peat, perlite, vermiculite and stalk pulverized particles.Chinese invention patent (application number 201610769583.3) a kind of secondary salinization of land improvement nutritional agents based on phosphorus preparation is disclosed, its raw material includes:Crops are given up Expect, it is the excrement that becomes thoroughly decomposed, nano-sized carbon, nano-calcium carbonate, purple coneflower leaf slag material, pyroligneous liquor, azotobacter chroococcum, fertilizer polymerizer, micro- Bio-fermentation agents.Chinese invention patent (application number 201610398210.X) discloses a kind of alkaline land improving fertilizer and its made Method, is that ardealite, sodium ion adsorbent and medical stone are mixed to obtain into A material;Acid conditioner, soluble humic acid and pyroligneous liquor Well mixed, obtained B liquid, the A being added in batch mixer is mixed evenly in expecting, obtains after slurry C, farmers' is put into thereto Fertilizer, straw powder are well mixed, and microbial bacterial agent, Tween-80 finally are put into well mixed in above-mentioned feed liquid produce.
In microbial bacterial agent preparation process, culture medium high-temperature sterilization consumption mass energy, while adding production cost. During organic fertilizer production, organic matter material decomposed period is long, and ammonia nitrogen therein is readily volatilized, not only loses nutrient, and dirty Air is contaminated, increases production cost.It can be seen that, the production cost of current microbial manure class saline-alkali soil conditioner is higher:One is Raw materials for production cost is higher, two be production or process energy consumption it is big, cause cost higher.
The content of the invention
In view of this, it is an object of the invention to propose a kind of microbial manure and preparation method thereof, microorganism fertilizer is reduced The production cost of material.
The preparation method of the microbial manure provided based on above-mentioned purpose, the present invention is comprised the following steps:
1) pH of regulation cassava alcohol waste water is 5.0~6.0, and sterilizing obtains trichoderma liquid seed culture medium;
2) trichoderma viride of slant preservation is inoculated with to trichoderma liquid seed culture medium, trichoderma bacterium solution is obtained after culture Body seed;
3) trichoderma liquid seed is inoculated with into cassava alcohol waste water, blowing air culture obtains trichoderma bacterium solution;
4) using the mixture of feces of livestock and poultry, bacteria residue and the stalk of crushing as solid culture based raw material, trichoderma bacterium solution is added Into solid culture based raw material, static state is banked up, blowing air culture, obtains product by solid-state fermentation;
5) processing is dried to product by solid-state fermentation, microbial manure is obtained.
In some embodiments of the invention, in step 1) in, the pH of cassava alcohol waste water is adjusted using limewash.
In some embodiments of the invention, in step 3) in, into cassava alcohol waste water be inoculated with volume ratio be 2%~ 10% trichoderma liquid seed, in 25~30 DEG C, blowing air culture 15~36 hours obtains trichoderma bacterium solution.
In some embodiments of the invention, in step 4) in, feces of livestock and poultry, bacteria residue, the weight ratio for the stalk crushed are 1 ︰ (0.3~1.2) ︰ (0.2~1.5).
In some embodiments of the invention, in step 4) in, use calcium superphosphate to adjust pH for 5.0~5.5.
In some embodiments of the invention, in step 4) in, trichoderma bacterium solution is added to according to weight ratio for 2%~10% In solid culture based raw material, stir, and control material water content is 60%~70%, static state is banked up, and blowing air culture 5~ 8 days.
In some embodiments of the invention, in step 4) in, in incubation every turning in 1~2 day once, and 55 DEG C maintained above 3~5 days, obtains product by solid-state fermentation.
In some embodiments of the invention, in step 4) in obtained product by solid-state fermentation Green Trichoderma with mitogenetic Based on spore, organic matter material is semi-decomposed state, and pH is 4.5~5.5.
In some embodiments of the invention, in step 5) in, natural draft drying processing is carried out to product by solid-state fermentation, Water content is set to reach less than 30%, spore content reaches 7 × 108Individual spore/gram more than.
The present invention also provides a kind of microbial manure, and the microbial manure is according to the preparation method of mentioned microorganism fertilizer Obtain.
The microbial manure that the present invention is provided, which is applied to salt affected soil improvement and agricultural production, can reduce soil moisture Evaporation accumulation it is saline and alkaline, strengthen plant anti-salt alkali ability, or replacing fertilizer and agricultural chemicals usage amount, change so as to meet salt affected soil Good and green agriculture production demand;And production cost is relatively low, provided cassava alcohol waste water and biomass castoff Sourceization is utilized, and reduces pollutant emission.Therefore, the present invention is not only able to reduce production in terms of raw materials for production and production process two Cost, while salt affected soil improved performance can also be improved.The microbial manure that the present invention is provided can promote crop to nutrient Absorb and utilize, controlling plant diseases, reduce the usage amount of chemical fertilizer and agricultural chemicals, so as to significantly improve farm environment quality, improve The quality of agricultural product.
Embodiment
For the object, technical solutions and advantages of the present invention are more clearly understood, below in conjunction with specific embodiment, to this hair It is bright to be further described.
Cassava alcohol can be widely applied to the industries such as medicine, chemical industry, food, and can be used as regenerative resource and cleaning energy Source, market potential is huge.But, during using cassava fermenting and producing alcohol, a large amount of high concentrated organic wastewaters can be produced (mg/litre of COD concentration numbers ten thousand), 1 ton of alcohol of production needs to discharge about 15 tons of high-concentration waste water, handles up to standard extremely difficult, place Manage cost high, manufacturing enterprise is difficult to bear.Cassava alcohol waste water is mainly the largely useless mash produced in alcohol production process, its Mainly contain remaining starch, sugar, crude protein, cellulose, various inorganic salts and thalline material etc..It can be given birth to as microbial bacterial agent The cheap raw material of production.The excrement and high-concentration waste water that the current livestock and poultry breeding industry of China is discharged every year reach billions of tons, and environment is dirty Dye is serious;More than annual 3000 ten thousand tons of China's edible mushroom yield, a large amount of bacteria residues also have become environmental problem urgently to be resolved hurrily, by this A little discarded objects, as the raw materials for production of saline alkali soil conditioner, are the effective ways of recycling.
The present invention starts with from higher and production or process the two the big links of energy consumption of raw materials for production cost, reduction life The energy consumption of production process, reduces nutrient loss, reduces production cost, improves the saline and alkaline performance of improved product.
Embodiment 1
The preparation method for the microbial manure that the present invention is provided comprises the following steps:
1) preparation of trichoderma liquid seed culture medium:The pH that cassava alcohol waste water is adjusted with limewash is 5.5, in 115 DEG C sterilizing 10 minutes, obtain trichoderma liquid seed culture medium.
2) prepared by trichoderma liquid seed:The trichoderma viride of slant preservation is inoculated with to trichoderma liquid seed culture medium, In 28 DEG C, 150 revs/min of shaken cultivations 24 hours obtain trichoderma liquid seed.
3) Trichoderma liquid culture:The wood that volume ratio is 5% is inoculated with into the fermentation tank equipped with fresh cassava alcohol waste water Mould liquid seeds, in 28 DEG C, blowing air culture 24 hours obtains trichoderma bacterium solution.Cultured trichoderma bacterium solution is sent out as solid-state Ferment seed liquor is used.In the incubation, fresh wastewater saves energy consumption without sterilizing.
4) solid state fermentation:Solid culture based raw material, three are used as using the mixture of feces of livestock and poultry, bacteria residue and the stalk of crushing Weight ratio is the ︰ 0.5 of 1 ︰ 0.8, is 5.5 with calcium superphosphate regulation pH;Trichoderma bacterium solution is added to solid training according to weight ratio for 10% Support in based raw material, stir, and control material water content is 65%, static state is banked up, blowing air culture 5~8 days, incubation In every turning in 1~2 day once, it is and maintained above 5 days at 55 DEG C, produce product by solid-state fermentation.In this process, material need not Become thoroughly decomposed and stabilize completely, member-retaining portion organic acid reduces the ammonia nitrogen discharge in feces of livestock and poultry, is conducive to salt affected soil to improve.
5) prepared by microbial manure:In step 4) in obtained product by solid-state fermentation Green Trichoderma using conidium as Main, organic matter material is semi-decomposed state, and pH is 5 or so;Handled through natural draft drying, water content is reached 25% or so, Spore content reaches 2 × 109Individual spore/gram or so, that is, obtain microbial manure.It can also further granulate, pack, be deposited in At cool place is dried.
Embodiment 2
The preparation method for the microbial manure that the present invention is provided comprises the following steps:
1) preparation of trichoderma liquid seed culture medium:The pH that cassava alcohol waste water is adjusted with limewash is 5.8, in 118 DEG C sterilizing 15 minutes, obtain trichoderma liquid seed culture medium.
2) prepared by trichoderma liquid seed:The trichoderma viride of slant preservation is inoculated with to trichoderma liquid seed culture medium, In 26 DEG C, 180 revs/min of shaken cultivations 30 hours obtain trichoderma liquid seed.
3) Trichoderma liquid culture:It is 6.5% that volume ratio is inoculated with into the fermentation tank equipped with fresh cassava alcohol waste water Trichoderma liquid seed, in 29 DEG C, blowing air culture 29 hours obtains trichoderma bacterium solution.Cultured trichoderma bacterium solution is used as solid-state Fermentation seed liquid is used.In the incubation, fresh wastewater saves energy consumption without sterilizing.
4) solid state fermentation:Solid culture based raw material, three are used as using the mixture of feces of livestock and poultry, bacteria residue and the stalk of crushing Weight ratio is the ︰ 0.8 of 1 ︰ 0.5, is 5.0 with calcium superphosphate regulation pH;Trichoderma bacterium solution is added to solid culture according to weight ratio for 3% In based raw material, stir, and control material water content is 63%, static state is banked up, blowing air culture 5~8 days, in incubation Every turning in 1~2 day once, it is and maintained above 4 days at 55 DEG C, produce product by solid-state fermentation.In this process, material is without complete Become thoroughly decomposed and stabilize entirely, member-retaining portion organic acid reduces the ammonia nitrogen discharge in feces of livestock and poultry, is conducive to salt affected soil to improve.
5) prepared by microbial manure:In step 4) in obtained product by solid-state fermentation Green Trichoderma using conidium as Main, organic matter material is semi-decomposed state, and pH is 5.2 or so;Handled through natural draft drying, water content is reached that 28% is left The right side, spore content reaches 1.2 × 109Individual spore/gram or so, that is, obtain microbial manure.It can also further granulate, pack, It is deposited at shady and cool dry.
Embodiment 3
The preparation method for the microbial manure that the present invention is provided comprises the following steps:
1) preparation of trichoderma liquid seed culture medium:The pH that cassava alcohol waste water is adjusted with limewash is 6.0, in 112 DEG C sterilizing 30 minutes, obtain trichoderma liquid seed culture medium.
2) prepared by trichoderma liquid seed:The trichoderma viride of slant preservation is inoculated with to trichoderma liquid seed culture medium, In 27 DEG C, 200 revs/min of shaken cultivations 33 hours obtain trichoderma liquid seed.
3) Trichoderma liquid culture:The wood that volume ratio is 10% is inoculated with into the fermentation tank equipped with fresh cassava alcohol waste water Mould liquid seeds, in 27 DEG C, blowing air culture 31 hours obtains trichoderma bacterium solution.Cultured trichoderma bacterium solution is sent out as solid-state Ferment seed liquor is used.In the incubation, fresh wastewater saves energy consumption without sterilizing.
4) solid state fermentation:Solid culture based raw material, three are used as using the mixture of feces of livestock and poultry, bacteria residue and the stalk of crushing Weight ratio is the ︰ 0.3 of 1 ︰ 1, is 5.1 with calcium superphosphate regulation pH;Trichoderma bacterium solution is added to solid medium according to weight ratio for 9% In raw material, stir, and control material water content is 60%, static state is banked up, blowing air culture 5~8 days, every in incubation Every turning in 1~2 day once, it is and maintained above 3 days at 55 DEG C, produce product by solid-state fermentation.In this process, material is without completely Become thoroughly decomposed and stabilize, member-retaining portion organic acid reduces the ammonia nitrogen discharge in feces of livestock and poultry, is conducive to salt affected soil to improve.
5) prepared by microbial manure:In step 4) in obtained product by solid-state fermentation Green Trichoderma using conidium as Main, organic matter material is semi-decomposed state, and pH is 4.5 or so;Handled through natural draft drying, water content is reached that 30% is left The right side, spore content reaches 1 × 109Individual spore/gram or so, that is, obtain microbial manure.It can also further granulate, pack, protect Ensconce at shady and cool dry.
Embodiment 4
The preparation method for the microbial manure that the present invention is provided comprises the following steps:
1) preparation of trichoderma liquid seed culture medium:The pH that cassava alcohol waste water is adjusted with limewash is 5.9, in 110 DEG C sterilizing 15 minutes, obtain trichoderma liquid seed culture medium.
2) prepared by trichoderma liquid seed:The trichoderma viride of slant preservation is inoculated with to trichoderma liquid seed culture medium, In 25 DEG C, 200 revs/min of shaken cultivations 20 hours obtain trichoderma liquid seed.
3) Trichoderma liquid culture:The wood that volume ratio is 2% is inoculated with into the fermentation tank equipped with fresh cassava alcohol waste water Mould liquid seeds, in 30 DEG C, blowing air culture 35 hours obtains trichoderma bacterium solution.Cultured trichoderma bacterium solution is sent out as solid-state Ferment seed liquor is used.In the incubation, fresh wastewater saves energy consumption without sterilizing.
4) solid state fermentation:Solid culture based raw material, three are used as using the mixture of feces of livestock and poultry, bacteria residue and the stalk of crushing Weight ratio is the ︰ 1.5 of 1 ︰ 0.3, is 5.3 with calcium superphosphate regulation pH;Trichoderma bacterium solution is added to solid culture according to weight ratio for 6% In based raw material, stir, and control material water content is 67%, static state is banked up, blowing air culture 5~8 days, in incubation Every turning in 1~2 day once, it is and maintained above 4 days at 55 DEG C, produce product by solid-state fermentation.In this process, material is without complete Become thoroughly decomposed and stabilize entirely, member-retaining portion organic acid reduces the ammonia nitrogen discharge in feces of livestock and poultry, is conducive to salt affected soil to improve.
5) prepared by microbial manure:In step 4) in obtained product by solid-state fermentation Green Trichoderma using conidium as Main, organic matter material is semi-decomposed state, and pH is 4.5 or so;Handled through natural draft drying, water content is reached that 27.5% is left The right side, spore content reaches 8 × 108Individual spore/gram or so, that is, obtain microbial manure.It can also further granulate, pack, protect Ensconce at shady and cool dry.
It should be noted that Trichoderma viride used in above-described embodiment can be commercially available or by inventor Plant height effect is separated from mushroom culture medium waste residue and promotes plant growth, the trichoderma viride of antagonistic phytopathogen (Trichoderma viride) EBL13 bacterial strains, the bacterial strain was deposited in Chinese microorganism strain preservation on October 18th, 2013 Administration committee's common micro-organisms center, deposit number is:CGMCC No.8363, depositary institution address:Chaoyang District, Beijing City north The institute 3 of occasion West Road 1, Institute of Microorganism, Academia Sinica, postcode:100101.
The application process for the microbial manure that the present invention is provided is as follows:
After autumn harvest, entered before the winter, according to every mu of 1-5 tons of consumption, be uniformly sprinkling upon on salt-soda soil, plough deeply, for nursing one's health soil Earth, reduces the evaporation accumulation of salt in the surface soil;Prior to seeding, according to every mu of 1-5 tons of consumption, uniformly it is sprinkling upon on salt-soda soil, ploughs deeply, makes as base manure With.
Comparative example:Salt affected soil improves application of the microbial manure in the repair process of salt-soda soil
In the farmland of Typical North salinized soil, selection sorghum growing way consistent experimental field 5 mu or so, field management It is consistent, simply soil conditioning is different.5 pieces are experimental field divided into, 5 processing are corresponded to respectively, between each processing Have provided with 2 meters or so of isolation strip.Bio-feritlizer prepared by the present invention is applied at salt affected soil, 5 according to different amounts Reason does not nurse one's health control (CK0) respectively, (CK3) is compareed using common organic fertilizer (3 tons/mu/year), using common organic fertilizer (5 Ton/mu/year) control (CK5), using low dosage microbial manure (3 tons/mu/year) conditioning (L3), administered with high dose microorganism fertilizer Material (5 tons/mu/year) conditioning (H5).Each processing applies the 50% of whole year amount of application in an identical manner before the winter is entered and is used as soil Nurse one's health, and the 50% of the annual amount of application of preceding administration is sowed in spring and be used as base manure.Field management is carried out according to unified approach, until Autumn sorghum is harvested, and whole process collection sample is analyzed.
As a result show:Compared with compareing CK0, all it is remarkably improved using the microbial manure of common organic fertilizer and the present invention Yield, CK3, CK5, L3, H5 increase production 16%, 23%, 37%, 51% respectively;Containing in the soil after distinct methods are nursed one's health in addition Salt amount all decreases than control.
It can be seen that, the microbial manure that the present invention is provided, which is applied to agricultural production, can be reduced or replacing fertilizer and agricultural chemicals Usage amount so that meet organic farm products production demand;And production cost is relatively low, make Sweet Potato Starch Processing Waste Water and sweet potato useless Gurry gets utilization, and reduces pollutant emission.Therefore, the present invention is not only able to from raw materials for production and the side of production process two Face reduces production cost, while salt affected soil improved performance can also be improved.
As can be seen here, the microbial manure that the present invention is provided can significantly alleviate influence of the salt affected soil to plant growth, Promote absorption and utilization of the crop to nutrient, improve farm environment quality, improve the quality of agricultural product, and can effectively anti-Zhiduo County Plant plant disease.
Those of ordinary skills in the art should understand that:The discussion of any of the above embodiment is exemplary only, not It is intended to imply that the scope of the present disclosure (including claim) is limited to these examples;Under the thinking of the present invention, above example Or can also not be combined between the technical characteristic in be the same as Example, step can be realized with random order, and be existed such as Many other changes of upper described different aspect of the invention, for simplicity, they are provided not in details.Therefore, it is all Within the spirit and principles in the present invention, any omission, modification, equivalent substitution, improvement for being made etc. should be included in the present invention's Within protection domain.

Claims (10)

1. a kind of preparation method of microbial manure, it is characterised in that comprise the following steps:
1) pH of regulation cassava alcohol waste water is 5.0~6.0, and sterilizing obtains trichoderma liquid seed culture medium;
2) trichoderma viride of slant preservation is inoculated with to trichoderma liquid seed culture medium, trichoderma liquid kind is obtained after culture Son;
3) trichoderma liquid seed is inoculated with into cassava alcohol waste water, blowing air culture obtains trichoderma bacterium solution;
4) using the mixture of feces of livestock and poultry, bacteria residue and the stalk of crushing as solid culture based raw material, trichoderma bacterium solution is added to solid In body culture medium raw material, static state is banked up, blowing air culture, obtains product by solid-state fermentation;
5) processing is dried to product by solid-state fermentation, microbial manure is obtained.
2. the preparation method of microbial manure according to claim 1, it is characterised in that in step 1) in, using lime Water adjusts the pH of cassava alcohol waste water.
3. the preparation method of microbial manure according to claim 1, it is characterised in that in step 3) in, to cassiri The trichoderma liquid seed that volume ratio is 2%~10% is inoculated with smart waste water, in 25~30 DEG C, blowing air culture 15~36 is small When, obtain trichoderma bacterium solution.
4. the preparation method of microbial manure according to claim 1, it is characterised in that in step 4) in, feces of livestock and poultry, Bacteria residue, the weight ratio for the stalk crushed are 1 ︰ (0.3~1.2) ︰ (0.2~1.5).
5. the preparation method of microbial manure according to claim 1, it is characterised in that in step 4) in, used phosphorus Sour calcium regulation pH is 5.0~5.5.
6. the preparation method of microbial manure according to claim 1, it is characterised in that in step 4) in, by Trichoderma Liquid is 2%~10% to be added in solid culture based raw material according to weight ratio, is stirred, and control material water content be 60%~ 70%, static state is banked up, blowing air culture 5~8 days.
7. the preparation method of microbial manure according to claim 1, it is characterised in that in step 4) in, cultivating In journey every turning in 1~2 day once, it is and maintained above 3~5 days at 55 DEG C, obtain product by solid-state fermentation.
8. the preparation method of microbial manure according to claim 1, it is characterised in that in step 4) in obtained solid-state Tunning Green Trichoderma is based on conidium, and organic matter material is semi-decomposed state, and pH is 4.5~5.5.
9. the preparation method of microbial manure according to claim 1, it is characterised in that in step 5) in, solid-state is sent out Ferment product carries out natural draft drying processing, water content is reached less than 30%, spore content reaches 7 × 108Individual spore/gram with On.
10. a kind of microbial manure, it is characterised in that the microbial manure is according to any one in claim 1~9 The preparation method of microbial manure obtain.
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CN107840706A (en) * 2017-10-17 2018-03-27 中国科学院生态环境研究中心 A kind of microbial manure and its application using the production of cassava alcohol waste water
CN108558484A (en) * 2017-10-24 2018-09-21 河北丰禾绿光农业科技有限公司 A kind of selenium-enriched microbe fertilizer and preparation method thereof
CN111320511A (en) * 2020-03-31 2020-06-23 王美娟 Soil fertilizer for preventing and treating soil-borne diseases and preparation method thereof
CN113277898A (en) * 2021-06-07 2021-08-20 海南瑞民农业科技有限公司 Special salt-containing organic fertilizer for coconut nursery stage
CN114457000A (en) * 2022-01-20 2022-05-10 中国林业科学研究院森林生态环境与自然保护研究所 Application of wood vinegar liquid in promoting Esteya vermicola blastospore germination to generate crescent spores
CN114835517A (en) * 2022-05-23 2022-08-02 河南康盈环保科技有限公司 Method for solid-state fermentation of trichoderma viride bio-organic fertilizer by utilizing kitchen waste

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CN102050645A (en) * 2009-10-27 2011-05-11 中国科学院生态环境研究中心 Method for producing trichoderma microbial fertilizer by utilizing sweet potato starch wastewater
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Cited By (6)

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Publication number Priority date Publication date Assignee Title
CN107840706A (en) * 2017-10-17 2018-03-27 中国科学院生态环境研究中心 A kind of microbial manure and its application using the production of cassava alcohol waste water
CN108558484A (en) * 2017-10-24 2018-09-21 河北丰禾绿光农业科技有限公司 A kind of selenium-enriched microbe fertilizer and preparation method thereof
CN111320511A (en) * 2020-03-31 2020-06-23 王美娟 Soil fertilizer for preventing and treating soil-borne diseases and preparation method thereof
CN113277898A (en) * 2021-06-07 2021-08-20 海南瑞民农业科技有限公司 Special salt-containing organic fertilizer for coconut nursery stage
CN114457000A (en) * 2022-01-20 2022-05-10 中国林业科学研究院森林生态环境与自然保护研究所 Application of wood vinegar liquid in promoting Esteya vermicola blastospore germination to generate crescent spores
CN114835517A (en) * 2022-05-23 2022-08-02 河南康盈环保科技有限公司 Method for solid-state fermentation of trichoderma viride bio-organic fertilizer by utilizing kitchen waste

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