CN102199051A - Trichoderma microbial fertilizer production by reutilizing livestock and poultry manure - Google Patents
Trichoderma microbial fertilizer production by reutilizing livestock and poultry manure Download PDFInfo
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- CN102199051A CN102199051A CN2010101301328A CN201010130132A CN102199051A CN 102199051 A CN102199051 A CN 102199051A CN 2010101301328 A CN2010101301328 A CN 2010101301328A CN 201010130132 A CN201010130132 A CN 201010130132A CN 102199051 A CN102199051 A CN 102199051A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention relates to a Trichoderma microbial fertilizer production method, and belongs to the technical fields of microbial fertilizer and livestock and poultry manure reutilization. According the method, livestock and poultry manure and biomass waste are used as production raw materials, and a liquid-solid combined fermentation method is adopted to produce Trichoderma microbial fertilizer. The production technology comprises the following steps: (1) livestock and poultry manure is used as medium to inoculate Trichoderma strains, and liquid seeds are prepared in a liquid culture; (2) livestock and poultry manure is used as fermentation raw material, and liquid seeds are inoculated to prepare liquid Trichoderma products by a large amount; (3) cultured liquid seeds or liquid Trichoderma products are added into a biomass material, and solid Trichoderma products are prepared after solid state fermentation; (4) after drying processing, the fermentation products are added with a stabilizing agent and an auxiliary agent, then crushing and package are carried out. Trichoderma microbial fertilizer prepared by the method has low production costs, is environment friendly, can obviously improve soil quality, raise crop output, and control a plurality of plant diseases. In addition, an effective processing method is found for the reutilization of livestock and poultry manure to reduce environment pollution.
Description
Technical field
The invention belongs to microbial fertilizer and utilization of waste as resource technical field.
Technical background
In recent years, China's intensification livestock and poultry breeding industry development is swift and violent, has obtained huge social benefit and economic benefit, has also produced a large amount of feces of livestock and poultry wastes simultaneously.The data presentation of national environmental protection portion, the annual feces of livestock and poultry total emission volumn of China surpasses 2,500,000,000 tons, has become the main source of rural area pollution of area source.Because most of plants do not have the prevention and cure of pollution facility, or environmental protection facility imperfection, a large amount of ight soil, sewage enter in the environment without effectively handling, polluted source and air, spread disease germs, a series of harm such as polluted agricultural land, brought for agriculture production and ecotope and seriously influence.And contain the good nutrition substrate that a large amount of organic matters are microorganism growth in the feces of livestock and poultry, can make microbiological culture media, the fermentative production microniological proudcts.Therefore, exploiting natural resources utilizes feces of livestock and poultry to produce the microbial fertilizer technology, not only can turn waste into wealth, reduce source of pollution, and the application of microbial fertilizer can also reduce the chemical fertilizer in the agriculture production and the usage quantity of agricultural chemicals, save energy is improved the quality of agricultural-food, compound developing a circular economy and the requirement of green agriculture.
The treatment process of the feces of livestock and poultry of bibliographical information mainly contains compost, fermentation methane, directly agricultural etc.Chinese patent (application number: disclose a kind of swine manure wastewater 200410060847.5) and handled the qualified discharge technology, can effectively control its contaminate environment.Chinese patent (application number: disclose a kind of livestock excrement composting treatment technology 200410060846.0), can produce high-quality fertilizer, be used to improve the soil.Do not see at present the report that utilizes feces of livestock and poultry to produce the Trichoderma microbial fertilizer.
Trichoderma (Trichoderma sp.) is imperfect fungi Moniliales fungi on classification position, widely distributed at occurring in nature, and wood is mould to have antagonistic action to multiple important plant pathogenic fungi, is acknowledged as the very effective biological control factor.Find again that in recent years the mould growth to various plants of art plays promoter action, therefore all develop some based on the mould bio-bacterial manure of wood both at home and abroad, in the promotion plant growth, improved the disease resistance of crop.Microbial fertilizer is meant that a class contains the particular formulations of living microorganism, is applied in the agriculture production, can obtain specific fertilizer effect.It can the activating soil nutrient, strengthens soil supplying nutrient capability, stimulates plant growth, improves crop yield and quality, prevents the breeding of harmful bacterium, increases beneficial microorganism quantity in the soil, to improving ecological environment of soil good promoter action is arranged.
The substratum that production method adopted of the Trichoderma of bibliographical information is main nutrient matter source with grain and byproduct thereof.As: (publication number: disclose the production method of a kind of Trichoderma CN 1554242A), its substratum mainly is millet, rice, corn, barley, wheat or the jowar of particle diameter between the 1-5 millimeter to Chinese patent.(publication number: disclose the production method of a kind of Trichoderma CN 1422946A), its raw materials for production mainly are barley, wheat bran, naked oats etc. to Chinese patent.And the method for producing Trichoderma as substratum with feces of livestock and poultry yet there are no report.Trichoderma can multiple Mierocrystalline cellulose of metabolism and hemicellulase, can effectively utilize various biomass, as: stalk, rice husk, bagasse, beet pulp etc.Contain a large amount of organic and inorganic nutrient elements in the feces of livestock and poultry, can provide multiple nutrients for the Trichoderma growth.Therefore, produce Trichoderma with feces of livestock and poultry and not only can reduce production costs, and can effectively handle organic waste.
Summary of the invention
The purpose of this invention is to provide a kind of method of utilizing feces of livestock and poultry and abandoned biomass (stalk, bagasse, beet pulp, rice husk etc.) to produce wooden mould microbial fertilizer.
The production method of the mould microbial fertilizer of wood of the present invention is as follows:
1, seed preparation: the Trichoderma kind is conventional agricultural bacterial classification as viride (Trichoderma viride), trichoderma harziarum (Trichoderma harziamum), yellow green trichoderma (Trichoderma auxeoviride), koning trichoderma (Trichodermakoningii) etc.The feces of livestock and poultry water content is adjusted to 80%-98%, in 105 ℃-126 ℃ high temperature steaming 15-30 minute, cooling back inoculation Trichoderma kind, inoculum density 10
6-10
7Individual spore/milliliter feces of livestock and poultry in 20-35 ℃, shakes bottle 100-200 rev/min and cultivated 24-72 hour, promptly gets liquid seed.
2, liquid state fermentation: the feces of livestock and poultry water content is adjusted to 80%-98%, in 105 ℃-126 ℃ high temperature steaming 15-30 minute.The liquid seed of inoculation 2%-20%, in 20-35 ℃, the aerobic cultivation of ventilation is 24-72 hour in the fermentor tank, promptly gets liquid Trichoderma product.
3, solid state fermentation: with abandoned biomass (stalk of pulverizing, bagasse, beet pulp, rice husk etc.) is amendment and upholder, cultured liquid seed or liquid Trichoderma product are added in the biomass amendment according to 2-4 times of weight, stir, in 20-35 ℃, static cultivation 3-20 days, forced ventilation or turning in 2 days are 1 time in the culturing process, promptly get solid-state Trichoderma product.
4, aftertreatment: liquid Trichoderma product adds microbial-bacterial fertilizer stablizer commonly used and auxiliary agent based on mycelium, and cryopreservation uses as early as possible.Solid-state Trichoderma product after natural draft drying is handled, adds microbial-bacterial fertilizer stablizer commonly used and auxiliary agent based on conidium, pulverizes packing.Also can directly be used in agricultural land soil according to the region situation, can increase soil fertility, can improve the soil again, effectively prevent and treat soil-borne disease.
Produce wooden mould microbial fertilizer by technical solution of the present invention, it is low to have a production cost, eco-friendly characteristics, and product can obviously improve soil environment quality, improve crop yield, and can prevent and treat plurality of plant diseases.Find effective treatment process for the efficient feces of livestock and poultry of handling simultaneously.Below describe enforcement of the present invention in detail by specific embodiment, purpose is to help the reader to understand spirit of the present invention better, but not as to the qualification of the scope of the present invention.
Embodiment 1
Feces of livestock and poultry liquid state-solid-state combined ferment is produced the viride microbial fertilizer:
The seed preparation: the feces of livestock and poultry water content is adjusted to 90%, in 115 ℃ of sterilizations 20 minutes, cooling back inoculation viride bacterial classification, inoculum density 10
7Individual spore/milliliter substratum in 26 ℃, shakes bottle and cultivated 36 hours for 150 rev/mins, promptly gets liquid seed.
Liquid state fermentation: the feces of livestock and poultry water content is adjusted to 90%, in 100 ℃ of boilings 20 minutes.Inoculate 5% liquid seed, in 26 ℃, the aerobic cultivation of ventilation is 36 hours in the fermentor tank, promptly gets liquid trichoderma viride product.
Solid state fermentation: with the waste in sugar refinery---bagasse is amendment and upholder, cultured liquid seed or liquid Trichoderma product are added in the biomass amendment according to 2.5 times of weight, stir, in 28 ℃, static cultivation 10 days, forced ventilation or turning in 2 days are 1 time in the culturing process, promptly get solid-state trichoderma viride product.
Aftertreatment: solid-state trichoderma viride product after natural draft drying is handled, adds microbial-bacterial fertilizer stablizer commonly used and auxiliary agent based on conidium, pulverizes packing.The product spore content can reach 6 * 10
9Individual spore/gram.
Embodiment 2
Feces of livestock and poultry liquid state-solid-state combined ferment is produced the trichoderma harziarum microbial fertilizer:
The seed preparation: the feces of livestock and poultry water content is adjusted to 90%, in 121 ℃ of sterilizations 15 minutes, cooling back inoculation trichoderma harziarum bacterial classification, inoculum density 10
6Individual spore/milliliter substratum in 28 ℃, shakes bottle and cultivated 48 hours for 150 rev/mins, promptly gets liquid seed.
Liquid state fermentation: the feces of livestock and poultry water content is adjusted to 90%, in 121 ℃ of sterilizations 15 minutes, inoculates 10% liquid seed, in 28 ℃, the aerobic cultivation of ventilation is 36 hours in the fermentor tank, promptly gets liquid trichoderma harzianum product.
Solid state fermentation: with the beet pulp is amendment and upholder, and cultured liquid seed or liquid Trichoderma product are added in the beet pulp according to 3 times of weight, stirs, and in 28 ℃, the static ventilation cultivated 10 days, promptly got solid-state trichoderma harzianum product.
Aftertreatment: solid-state trichoderma harzianum product after natural draft drying is handled, adds microbial-bacterial fertilizer stablizer commonly used and auxiliary agent based on conidium, pulverizes packing.The product spore content reaches 3 * 10
9Individual spore/gram.
Claims (1)
1. method of utilizing feces of livestock and poultry to produce the Trichoderma microbial fertilizer, it is characterized in that, this method is to produce wooden mould microbial fertilizer with the feces of livestock and poultry for the main medium raw material carries out liquid state-solid-state combined ferment, and the production method of the mould microbial fertilizer of described wood comprises the steps:
(1) seed preparation: the Trichoderma kind is conventional agricultural bacterial classification, comprising: viride (Trichoderma viride), trichoderma harziarum (Trichoderma harziamum), yellow green trichoderma (Trichoderma auxeoviride), koning trichoderma (Trichoderma koningii).The feces of livestock and poultry water content is adjusted to 80%-98%, in 105 ℃-126 ℃ high temperature steaming 15-30 minute, cooling back inoculation Trichoderma kind, inoculum density 10
6-10
7Individual spore/milliliter feces of livestock and poultry in 20-35 ℃, shakes bottle 100-200 rev/min and cultivated 24-72 hour, obtains liquid seed.
(2) liquid state fermentation: the feces of livestock and poultry water content is adjusted to 80%-98%, in 105 ℃-126 ℃ high temperature steaming 15-30 minute.The liquid seed of inoculation 2%-20%, in 20-35 ℃, the aerobic cultivation of ventilation is 24-72 hour in the fermentor tank, promptly gets liquid Trichoderma product.
(3) solid state fermentation: with abandoned biomass (stalk of pulverizing, bagasse, beet pulp, rice husk) is amendment and upholder, cultured liquid seed or liquid Trichoderma product are added in the biomass amendment according to 2-4 times of weight, stir, in 20-35 ℃, static cultivation 3-20 days, forced ventilation or turning in 2 days are 1 time in the culturing process, promptly get solid-state Trichoderma product.
(4) aftertreatment: liquid Trichoderma product adds microbial-bacterial fertilizer stablizer commonly used and auxiliary agent, cryopreservation.Solid-state Trichoderma product adds microbial-bacterial fertilizer stablizer commonly used and auxiliary agent after natural draft drying is handled, pulverize packing.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103408335A (en) * | 2013-08-13 | 2013-11-27 | 广西壮族自治区农业科学院葡萄与葡萄酒研究所 | Microbiological method based harmlessness treatment technology for agricultural wastes generated by grape cultivation |
| CN103772056A (en) * | 2014-02-18 | 2014-05-07 | 山东粮源生物有机肥有限公司 | Organic bioactive fertilizer produced by popping technology and preparation method thereof |
| CN103910545A (en) * | 2014-03-18 | 2014-07-09 | 福建省农业科学院农业生物资源研究所 | Method for using pig manure waste to produce trichoderma harzianum fertilizer |
| CN106008087A (en) * | 2016-05-19 | 2016-10-12 | 宁夏泰瑞制药股份有限公司 | Microorganism bacterium fertilizer and preparation method thereof |
| CN106069683A (en) * | 2016-06-29 | 2016-11-09 | 固镇县华原家庭农场 | A kind of Herba Apii graveolentis selenium-rich seedling medium |
| CN106069339A (en) * | 2016-06-29 | 2016-11-09 | 安徽省固镇县谷阳有机蔬菜专业合作社 | A kind of Sweet Pepper Seedlings substrate of diseases prevention growth promoting |
| CN106134717A (en) * | 2016-06-29 | 2016-11-23 | 固镇县华原家庭农场 | A kind of implantation methods of high yield high-quality Herba Apii graveolentis |
| CN106883998A (en) * | 2017-03-23 | 2017-06-23 | 河南工业大学 | A kind of preparation method of microorganism multifunctional fertilizer is constituted with fermentation medium |
| CN107129327A (en) * | 2017-04-11 | 2017-09-05 | 岳阳绿光农业科技有限公司 | Microbial manure and preparation method thereof |
| CN110156511A (en) * | 2019-04-12 | 2019-08-23 | 浙江省农业科学院 | A kind of biological organic fertilizer and preparation method thereof based on mineral humus and vegetables residuum trichoderma fermentation material |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1594538A (en) * | 2004-07-08 | 2005-03-16 | 中国海洋大学 | Microbial community and its preparation method and use |
| CN101204159A (en) * | 2006-12-22 | 2008-06-25 | 中国科学院生态环境研究中心 | Biological control agents for plant diseases extracting from high concentration organic wastewater |
| CN101391911A (en) * | 2008-11-07 | 2009-03-25 | 四川天正化工有限责任公司 | Method for preparing organic fertilizer by using aminonitrile waste liquid |
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2010
- 2010-03-23 CN CN2010101301328A patent/CN102199051A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1594538A (en) * | 2004-07-08 | 2005-03-16 | 中国海洋大学 | Microbial community and its preparation method and use |
| CN101204159A (en) * | 2006-12-22 | 2008-06-25 | 中国科学院生态环境研究中心 | Biological control agents for plant diseases extracting from high concentration organic wastewater |
| CN101391911A (en) * | 2008-11-07 | 2009-03-25 | 四川天正化工有限责任公司 | Method for preparing organic fertilizer by using aminonitrile waste liquid |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103408335A (en) * | 2013-08-13 | 2013-11-27 | 广西壮族自治区农业科学院葡萄与葡萄酒研究所 | Microbiological method based harmlessness treatment technology for agricultural wastes generated by grape cultivation |
| CN103772056A (en) * | 2014-02-18 | 2014-05-07 | 山东粮源生物有机肥有限公司 | Organic bioactive fertilizer produced by popping technology and preparation method thereof |
| CN103772056B (en) * | 2014-02-18 | 2016-01-20 | 山东粮源生物有机肥有限公司 | Organic biological active fertilizer utilizing swollen quick-fried technology to produce and preparation method thereof |
| CN103910545A (en) * | 2014-03-18 | 2014-07-09 | 福建省农业科学院农业生物资源研究所 | Method for using pig manure waste to produce trichoderma harzianum fertilizer |
| CN103910545B (en) * | 2014-03-18 | 2016-05-18 | 福建省农业科学院农业生物资源研究所 | Utilize pig manure discarded object to produce the method for Trichoderma harzianum bacterial manure |
| CN106008087A (en) * | 2016-05-19 | 2016-10-12 | 宁夏泰瑞制药股份有限公司 | Microorganism bacterium fertilizer and preparation method thereof |
| CN106069683A (en) * | 2016-06-29 | 2016-11-09 | 固镇县华原家庭农场 | A kind of Herba Apii graveolentis selenium-rich seedling medium |
| CN106069339A (en) * | 2016-06-29 | 2016-11-09 | 安徽省固镇县谷阳有机蔬菜专业合作社 | A kind of Sweet Pepper Seedlings substrate of diseases prevention growth promoting |
| CN106134717A (en) * | 2016-06-29 | 2016-11-23 | 固镇县华原家庭农场 | A kind of implantation methods of high yield high-quality Herba Apii graveolentis |
| CN106883998A (en) * | 2017-03-23 | 2017-06-23 | 河南工业大学 | A kind of preparation method of microorganism multifunctional fertilizer is constituted with fermentation medium |
| CN106883998B (en) * | 2017-03-23 | 2021-01-19 | 河南工业大学 | Preparation method of multifunctional microbial fertilizer and fermentation medium composition |
| CN107129327A (en) * | 2017-04-11 | 2017-09-05 | 岳阳绿光农业科技有限公司 | Microbial manure and preparation method thereof |
| CN110156511A (en) * | 2019-04-12 | 2019-08-23 | 浙江省农业科学院 | A kind of biological organic fertilizer and preparation method thereof based on mineral humus and vegetables residuum trichoderma fermentation material |
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Application publication date: 20110928 |