CN105948842A - Resource production of Bacillus licheniformis microbial fertilizer by utilizing excrements of livestock - Google Patents
Resource production of Bacillus licheniformis microbial fertilizer by utilizing excrements of livestock Download PDFInfo
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- CN105948842A CN105948842A CN201610284586.8A CN201610284586A CN105948842A CN 105948842 A CN105948842 A CN 105948842A CN 201610284586 A CN201610284586 A CN 201610284586A CN 105948842 A CN105948842 A CN 105948842A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention relates to a low-cost production method for producing a Bacillus licheniformis microbial fertilizer based on the resource utilization of the excrements of livestock, wherein the Bacillus licheniformis microbial fertilizer prevents and cures plant diseases and promotes the plant growth. The production method belongs to the technical field of livestock excrement treatment and microbial fertilizers. According to the technical scheme of the invention, a bacterial strain, separated and screened from the soil, is an efficient plant growth-promoting bacterium named Bacillus licheniformis strain B4. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) and the preservation number of the strain is CGMCC No. 6677. Meanwhile, the excrements of livestock and the starch wastewater are adopted to form a fermentation culture medium and the fermentation culture medium is used for the large-scale culture of Bacillus licheniformis. In this way, the microbial fertilizer capable of preventing and curing plant diseases and promoting the plant growth is prepared. The above production process is low in cost and simple in operation. The resource utilization of the excrements of livestock is realized and the pollutant discharge is reduced. The produced microbial fertilizer is used for the production of tea leaves and vegetables. By utilizing the microbial fertilizer, not only the yield is increased, but also the quality of a tea garden and vegetables is improved. Therefore, the microbial fertilizer has a wide application prospect.
Description
Technical field
The invention belongs to poultry excrement and urine disposal and microbial manure technical field.
Technical background
In recent years, the development of China's intensive livestock and poultry breeding industry is swift and violent, it is thus achieved that huge Social benefit and economic benefit, also creates substantial amounts of animal faeces wastes simultaneously.The data in national environmental protection portion show, the annual wastes from livestock and poultry of China, more than 2,500,000,000 tons, has become as the main source of Rural Plane Source Pollution.Owing to most of plants do not have Pollution treatment installation, or environmental protection facility imperfection, a large amount of feces, sewage enter in environment without effectively process, polluted source and air, spread disease germs, a series of harm such as polluted agricultural land, serious impact is brought, it is therefore necessary to carry out harmless treatment to agricultural production and ecological environment.It addition, feces of livestock and poultry is the good nutrition substrate of growth of microorganism containing a large amount of organic matters, microbiological culture media, fermenting and producing microniological proudcts can be made.Therefore, exploiting natural resources utilizes feces of livestock and poultry to produce microbial manure technology, it is possible not only to turn waste into wealth, reduce polluter, and the application of microbial manure can also reduce the usage amount of the chemical fertilizer in agricultural production and pesticide, save the energy, improve the quality of agricultural product, Complex Development recycling economy and the requirement of green agriculture.
The processing method of feces of livestock and poultry of document report mainly has compost, fermentation methane, the most agricultural etc..Chinese patent (application number: 200410060847.5) discloses a kind of swine manure wastewater and processes qualified discharge technology, can effectively control it and pollute environment.Chinese patent (application number: 200410060846.0) discloses a kind of livestock excrement composting treatment technology, the fertilizer of high-quality can be produced, be used for improveing soil.Chinese patent (application number: 200610089709.9) discloses a kind of animal dung and liquid dung fast harmless biological treating process, use waxy Bacillus, colloid bacillus cereus, Trichoderma spp., Bacillus coagulans, yeast, the complex micro organism fungicide of bacillus subtilis composition, it is inoculated in feces, add inserts, fermented after form fertilizer.Chinese patent (application number: 200810153855.2) discloses a kind of harmless treatment method for livestock manure, be feces of livestock and poultry is mixed with straw, Caulis et Folium Oryzae, sawdust after, addition farm manure and zymophyte powder, compost fermentation forms.Disclosing the preparation method of a kind of microorganism-decomposing agent for producing biological organic fertilizer by livestock and poultry manure fermentation in Chinese patent (application number: 201010247234.4), this decomposing agent includes: beer yeast, bacillus acidophilus, Rhodopseudomonas palustris.Chinese patent (application number: 201010594669.X) discloses a kind of harmless treatment method for livestock manure, cultivates four steps including animal wastes fermentation, fly maggot breeding, biogas preparation and microorganism.
Bacillus licheniformis (Bacillus licheniformis) is widely distributed in nature, it it is a kind of important biocontrol microorganisms, it suppresses plant pathogenetic bacteria, fungus, virus, nematicide by producing antibiotic, antibacterial protein or polypeptide isoreactivity material, thus plays the effect of Biological control；It is also the plant rhizosphere growth-promoting antibacterial that a class is important simultaneously, shows the effect that various plants promotes growth.Such as, Chinese invention patent (application number: 200310115535.5) discloses a bacillus licheniformis B-0A12 bacterial strain, deposit number is CGMCC No.1035, for preventing and treating soybean cyst nematode and the microbial root rot of reaping hook, the culture medium used in this strain fermentation incubation is: soluble starch 1-2%, soybean cake powder 1-2%, Carnis Bovis seu Bubali cream 0.5-1.0%, glucose 0.1-0.5%, the most a small amount of inorganic salt, during fermentation ends, bacterial content is 7 × 108Individual/milliliter.Chinese invention patent (application number: 200810026137.9) discloses a plants endogenetic Bacillus licheniformis R21 bacterial strain, deposit number is CCTCC M 207113, there is wide spectrum antagonistic plant pathogenic fungi ability, dissolving P capacity and promotion plant growing ability, its fermentation have employed LB culture medium, and disclose its preventing and treating Herba Apii graveolentis verticillium wilt, banana blight, and promote the application in terms of Herba Apii graveolentis growth.Chinese invention patent (application number: 200810183072.9) discloses bacillus licheniformis 201 bacterial strain, deposit number is CCTCC No.M207092, this bacterial strain has antagonism to multiple pathogenic fungi such as banana blight, watermelon blight, tomato wilt, banana anthracnose, wheat scabs, and the cultivation of this bacterial strain uses beef-protein medium.Disclosing bacillus licheniformis 202 bacterial strain in Chinese invention patent (application number: 200810183075.2), deposit number is CCTCC No.M207093, and it also has the biological and ecological methods to prevent plant disease, pests, and erosion function similar with 201 bacterial strains.
The Bacillus licheniformis also ratio of application industrially is wide.Such as, Chinese invention patent (application number: 200810227858.6) discloses a bacillus licheniformis U1-3 bacterial strain, deposit number is CGMCC No.2437, this bacterial strain can be used to the displacement of reservoir oil in petroleum production engineering field, improving oil recovery, its culture medium have employed liquid paraffin, yeast powder and plurality of inorganic salt.Chinese invention patent (application number: 200810055068.4) discloses a bacillus licheniformis WX-02 bacterial strain, deposit number is CCTCC No.M208065, for producing Polyurethane-epoxy resin, its culture medium have employed sodium glutamate, carbon source (one in starch or starch hydrolyzate, glucose, fructose, maltose, sucrose), inorganic nitrogen-sourced and other plurality of inorganic salt.Disclosing a bacillus licheniformis HM-3 bacterial strain in Chinese invention patent (application number: 200810026812.8), deposit number is CGMCC No.1970, is used for producing alkaline protease, and culture medium have employed yeast extract, peptone and municipal sludge.Chinese invention patent (application number: 200610053548.8) discloses a bacillus licheniformis B-05571 bacterial strain, deposit number is CCTCC No.M206082, for converting glycerol into dihydroxy acetone, culture medium with glycerol as primary carbon source, also yeast powder, peptone, Carnis Bovis seu Bubali cream, plurality of inorganic salt.
Have no that recycling feces of livestock and poultry produces the report of Bacillus licheniformis (Bacillus licheniformis) microbial manure at present.Utilize feces of livestock and poultry to carry out grown place clothing micro-organisms bacillus fertilizer to be possible not only to reduce production cost, and can be with this type of organic waste of recycling treatment.
The definition in agricultural industry criteria " microbial manure term " (NY/T 1113-2006) according to microbial manure, microbial manure is the goods containing specified microorganisms live body, it is applied to agricultural production, vital movement by microorganism contained therein, increase the supply of plant nutrient or promote plant growing, improve yield, improve quality of agricultural product and agroecological environment.At present, microbial manure includes microbial inoculant, composite microbiological fertilizer and biological organic fertilizer.
Summary of the invention
It is an object of the invention to provide a kind of harmless treatment feces of livestock and poultry and for the method producing lichens micro-organisms bacillus fertilizer.
Bacterial strain used in the present invention is to be promoted plant growing, Bacillus licheniformis (Bacillus licheniformis) the B4 bacterial strain of antagonistic phytopathogen by inventor's separation screening plant height effect out from soil, this bacterial strain is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on October 15th, 2012, deposit number is: CGMCC No.6677, depositary institution address: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101.
The method that feces of livestock and poultry harmless treatment of the present invention produces lichens micro-organisms bacillus fertilizer is as follows:
1, prepared by fluid medium: adding such as the starch wastewater of starch production process discharge in feces of livestock and poultry, the percentage by weight of regulation to solid content is 4%～8%, pulls an oar with refiner, 115 DEG C of autoclavings 15～30 minutes.
2, prepared by liquid spawn: inoculate the Bacillus licheniformis of slant preservation in the triangular flask of the fluid medium good equipped with pretreatment, in 30～37 DEG C, and 120～200 revs/min of shaken cultivation 20～36 hours, obtain liquid seeds.
3, liquid spawn amplification culture: inoculate the liquid spawn that volume ratio is 3%～10% in the fermentation tank of the fluid medium good equipped with pretreatment, in 30～37 DEG C, logical filtrated air is cultivated 18～36 hours；Cultured fermentation liquor count plate, viable count reaches 2 × 109More than CFU/ milliliter, can be as liquid microbe Fertilizer application.
4, solid fermentation: with pulverize straw, sawdust or rice husk as conditioner, mix according to weight ratio 1:5～3:1 with feces of livestock and poultry, after making mixing, the water content of material is 50%～55%, cultured liquid microbe fertilizer is added in mixed material according to the inoculum concentration that weight ratio is 5%～10%, stir, static culture 10～15 days, forced ventilation or turning in 1～3 day 1 time in incubation, hot stage controls temperature of charge between 56～68 DEG C, maintain more than 5 days, it is ensured that kill the pathogen in feces of livestock and poultry and parasitic ovum；I.e. obtain microbial manure primary product.
5, the preparation of solid microbe fertilizer: add the liquid microbe fertilizer that third step obtains in microbial manure primary product in right amount, make the viable count of Bacillus licheniformis in product reach 5 × 108More than CFU/ gram, aeration-drying is to water content less than 25%；Packaging, leaves shady and cool being dried in and locates.
Detailed description of the invention:
The microbial manure that the technical scheme that the present invention provides produces is applied to agricultural production and can reduce the usage amount of chemical fertilizer and pesticide, thus improves farm environment quality；And production cost is the lowest, make high-concentration waste water get utilization, reduce pollutant emission.Detailed description of the invention is as follows:
Embodiment 1: livestock and poultry dung recovery process for producing Bacillus licheniformis microbial manure
1, prepared by fluid medium: to the starch wastewater of feces of livestock and poultry Zhong Jia Starch Production enterprise discharge, and the percentage by weight regulated to solid content is 6%, pulls an oar with refiner, 115 DEG C of autoclavings 20 minutes.
2, prepared by liquid spawn: inoculate the Bacillus licheniformis of slant preservation in the triangular flask of the fluid medium good equipped with pretreatment, in 33 DEG C, and 150 revs/min of shaken cultivation 26 hours, obtain liquid seeds.
3, liquid spawn amplification culture: inoculating volume ratio in the fermentation tank of the fluid medium good equipped with pretreatment is the liquid spawn of 5%, in 33 DEG C, logical filtrated air is cultivated 20 hours；Cultured fermentation liquor count plate, viable count reaches 2 × 109More than CFU/ milliliter, can be as liquid microbe Fertilizer application.
4, solid fermentation: with pulverize straw, sawdust or rice husk as conditioner, mix according to weight ratio 1:1 with feces of livestock and poultry, after making mixing, the water content of material is 50%～55%, cultured liquid microbe fertilizer is added in mixed material according to the inoculum concentration that weight ratio is 8%, stir, static culture 12 days, forced ventilation or turning in 1～3 day 1 time in incubation, hot stage controls temperature of charge between 56～68 DEG C, maintain more than 5 days, it is ensured that kill the pathogen in feces of livestock and poultry and parasitic ovum；I.e. obtain microbial manure primary product.
5, the preparation of solid microbe fertilizer: add the liquid microbe fertilizer that third step obtains in microbial manure primary product in right amount, make the viable count of Bacillus licheniformis in product reach 5 × 108More than CFU/ gram, aeration-drying is to water content less than 25%；Packaging, leaves shady and cool being dried in and locates.
Embodiment 2: Bacillus licheniformis microbial manure is applied to Tea Production
Select one piece of Camellia sinensis growing way uniform experimental field in tea place, being evenly dividing is 6 communities, about 200 square metres, each community, it is respectively set to comparison (CK) of not applying fertilizer, application of organic fertilizers (OF) and use microbial manure (MF) 3 kinds process, every kind of process 2 is parallel, the isolation strip of about 2 meters it is designed with between each process, fertilizer and microbial manure are according to the consumption of 500 kilograms every mu, ditch spread 1 time before entering the winter, gather spring Folium Camelliae sinensis, result shows: the spring tea yield increased volume increase about 17% of application of organic fertilizers, and use the spring tea yield increased volume increase about 35% of microbial manure, and use water extraction in the Folium Camelliae sinensis of microbial manure, tea polyphenols and amino acid whose content are significantly higher than comparison.
Claims (2)
1. a livestock and poultry dung recovery utilizes and produces Bacillus licheniformis (Bacillus licheniformis) microorganism
The method of fertilizer, it is characterised in that the method comprises the steps:
(1) prepared by fluid medium: add such as the starch wastewater of starch production process discharge in feces of livestock and poultry,
Regulation is 4%～8% to the percentage by weight of solid content, pulls an oar with refiner, 115 DEG C of high pressure
Sterilizing 15～30 minutes；
(2) prepared by liquid spawn: inoculates inclined-plane in the triangular flask of the fluid medium good equipped with pretreatment and protects
The Bacillus licheniformis hidden, in 30～37 DEG C, 120～200 revs/min of shaken cultivation 20～36 hours,
Obtain liquid seeds；
(3) liquid spawn amplification culture: inoculum in the fermentation tank of the fluid medium good equipped with pretreatment
Long-pending ratio is the liquid spawn of 3%～10%, and in 30～37 DEG C, logical filtrated air cultivates 18～36
Hour；Cultured fermentation liquor count plate, viable count reaches 2 × 109CFU/ milliliter with
On, can be as liquid microbe Fertilizer application；
(4) solid fermentation: with pulverize straw, sawdust or rice husk as conditioner, with feces of livestock and poultry according to weight
Measuring and mix than 1:5～3:1, after making mixing, the water content of material is 50%～55%, by cultured
Liquid microbe fertilizer adds in mixed material according to the inoculum concentration that weight ratio is 5%～10%, stirs
Mix uniformly, static culture 10～15 days, forced ventilation or turning in 1～3 day 1 time in incubation,
Hot stage control temperature of charge, between 56～68 DEG C, maintains more than 5 days, it is ensured that kill poultry
Pathogen in feces and parasitic ovum；I.e. obtain microbial manure primary product；
(5) preparation of solid microbe fertilizer: add third step in microbial manure primary product in right amount
The liquid microbe fertilizer obtained, makes the viable count of Bacillus licheniformis in product reach 5 × 108
More than CFU/ gram, aeration-drying is to water content less than 25%；Packaging, leaves shady and cool being dried in and locates.
2. the Bacillus licheniformis (Bacillus licheniformis) described in claim 1, it is characterised in that should
Bacterial strain was deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms on October 15th, 2012
The heart, deposit number is: CGMCC No.6677.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108558484A (en) * | 2017-10-24 | 2018-09-21 | 河北丰禾绿光农业科技有限公司 | A kind of selenium-enriched microbe fertilizer and preparation method thereof |
| CN109020644A (en) * | 2018-09-05 | 2018-12-18 | 河北大学 | Utilize the method and application of Pig raising wastewater fermenting and producing microbial manure |
| CN109456091A (en) * | 2018-09-05 | 2019-03-12 | 河北大学 | Utilize the method and application of cowboying wastewater fermentation production microbial manure |
| CN114940635A (en) * | 2022-06-08 | 2022-08-26 | 中资蓝天生态环境科技有限公司 | Method for reducing emission of greenhouse gas by organic solid waste semipermeable membrane fermentation and preparing microbial fertilizer |
| CN115786171A (en) * | 2022-09-20 | 2023-03-14 | 鲁东大学 | Alcaligenes faecalis and application of bacterial agent thereof in reducing pathogenic bacteria in treatment of livestock and poultry manure |
| CN117736036A (en) * | 2024-02-04 | 2024-03-22 | 广东中施龙泰低碳科技有限公司 | Method for preparing bio-organic fertilizer by recycling earthworm feces |
| EP4182289A4 (en) * | 2020-07-15 | 2024-09-04 | Locus Solutions Ipco Llc | Improved methods and compositions for processing manure |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820349A (en) * | 2012-11-16 | 2014-05-28 | 中国科学院生态环境研究中心 | Bacillus licheniformis and preparation method and application of fungicide |
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- 2016-05-03 CN CN201610284586.8A patent/CN105948842B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820349A (en) * | 2012-11-16 | 2014-05-28 | 中国科学院生态环境研究中心 | Bacillus licheniformis and preparation method and application of fungicide |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108558484A (en) * | 2017-10-24 | 2018-09-21 | 河北丰禾绿光农业科技有限公司 | A kind of selenium-enriched microbe fertilizer and preparation method thereof |
| CN109020644A (en) * | 2018-09-05 | 2018-12-18 | 河北大学 | Utilize the method and application of Pig raising wastewater fermenting and producing microbial manure |
| CN109456091A (en) * | 2018-09-05 | 2019-03-12 | 河北大学 | Utilize the method and application of cowboying wastewater fermentation production microbial manure |
| EP4182289A4 (en) * | 2020-07-15 | 2024-09-04 | Locus Solutions Ipco Llc | Improved methods and compositions for processing manure |
| CN114940635A (en) * | 2022-06-08 | 2022-08-26 | 中资蓝天生态环境科技有限公司 | Method for reducing emission of greenhouse gas by organic solid waste semipermeable membrane fermentation and preparing microbial fertilizer |
| CN115786171A (en) * | 2022-09-20 | 2023-03-14 | 鲁东大学 | Alcaligenes faecalis and application of bacterial agent thereof in reducing pathogenic bacteria in treatment of livestock and poultry manure |
| CN117736036A (en) * | 2024-02-04 | 2024-03-22 | 广东中施龙泰低碳科技有限公司 | Method for preparing bio-organic fertilizer by recycling earthworm feces |
| CN117736036B (en) * | 2024-02-04 | 2024-08-23 | 广东中施龙泰低碳科技有限公司 | Method for preparing bio-organic fertilizer by recycling earthworm feces |
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