CN104609995B - Plant growth promoting bio-organic fertilizer for saline-alkali land - Google Patents
Plant growth promoting bio-organic fertilizer for saline-alkali land Download PDFInfo
- Publication number
- CN104609995B CN104609995B CN201510030096.0A CN201510030096A CN104609995B CN 104609995 B CN104609995 B CN 104609995B CN 201510030096 A CN201510030096 A CN 201510030096A CN 104609995 B CN104609995 B CN 104609995B
- Authority
- CN
- China
- Prior art keywords
- fermentation
- organic fertilizer
- salt
- bacillus subtilis
- growth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses plant growth promoting bio-organic fertilizer for saline-alkali land, and belongs to the technology of soil improvement. The bio-organic fertilizer produced by fermenting agricultural organic solid wastes through microbial strains can increase the survival rate and promote growth of salt-tolerant plants planted in mud-flat soil and increases a soil fertility improvement speed and speeds up an improvement progress. Compost is subjected to secondary fermentation by trichoderma asperellum 12 and bacillus subtilis D9, wherein the content of trichoderma asperellum 12 in a product is larger than or equal to 0.2*10＜8＞ cfu (colony forming unit) g＜-1＞, the content of bacillus subtilis D9 in the product is larger than or equal to 0.5*10＜8＞ cuf g＜-1＞, a pH value is 5.5, the mass ratio of the water content is smaller than or equal to 30% and the mass ratio of organic matter content is larger than or equal to 45%. An experiment indicates that compared with treatment without application of the bio-organic fertilizer, the biomass of salt-tolerant plants including suaeda galuce, cyperus malaccensis and kochia scoparia planted in soil is increased by 49.1%, 56.3% and 52.2 respectively after the bio-organic fertilizer is applied to the mud-flat saline-alkali soil.
Description
Technical field
The present invention relates to a kind of salt-soda soil growth-promoting biological organic fertilizer, belong to microbial technology field.
Background technology
There is 1.8 ten thousand kilometers of continent water front in China, because Sediment Siltation forms a large amount of beach soils, mud-flat soil high-quality
Exploitation, using the teaching of the invention it is possible to provide substantial amounts of agricultural land, be society bring huge economic benefit and ecological environment benefit.
Improvement main method in beach saline-alkali ground is, by the plantation salt-tolerant plant that digs trenches to drain the water away, to dig trenches to drain the water away and be conducive to moisture
Infiltrate, accelerate desalination.It is used for eluting the fresh water of soil salt due to most beach, relies primarily on precipitation, and precipitation is main
The summer of the 6-8 months, short time substantial amounts of precipitation are concentrated on, if the vegetation of salt-soda soil mud-flat soil covering is less, the big portion of rainfall
Dividing will be flowed away in the form of rainwash, it is difficult to reach the purpose for infiltrating the desalinization of soil by flooding or leaching.And except 6-8 months, other in months soil
Transpiration rate be precipitation several times, it is impossible to it is effective to reduce earth's surface transpiration, it is easy to cause the soil accumulation of salt in the surface soil.Therefore, plant
Salt tolerant crop is planted, there is conclusive influence for the improved speed of mud-flat soil.
Beach saline-alkali ground is poor due to structure, and soil beneficial microbe content is less, the salt-tolerant plant speed of growth of plantation
Slowly, biomass is relatively low, the serious improvement that have impact on soil.Growth-promoting microorganism (Plant Growth Promoting
Rhizobacteria, PGPR or Plant Growth Promoting Fungi, PGPF) it has been widely used in promotion plant
The growth of thing, because growth-promoting microorganism can secrete plant hormone, fixed nitrogen or activation phosphorus element etc..But beach saline-alkali
Ground, salt content is high, and soil transpiration rate is fast, and the resistance requirement to functional microorganism is higher.
Document 1 is the granted patent of publication number CN201310307561.1, discloses a kind of biology of prevention and control rice sheath blight disease
Preventing and treating bacterial strain, biological organic fertilizer and preparation method thereof, invention is using the spine spore for having notable biological control effect to rice sheath blight disease
Trichoderma strain 12 (Trichoderma asperellum12), using the biocontrol strains and rice husk, stalk, feces of livestock and poultry heap
Fertilizer is made bio-organic fertilizer, and 0.5 × 10 is contained in fertilizer8 cfu g-1More than the bacterial strain resting spore, water content be less than
15%th, the content of organic matter is more than 35%.
Obviously, document 1 provides a kind of microbial organic fertilizer of biological and ecological methods to prevent plant disease, pests, and erosion rice sheath blight disease, rather than promotes beach saline-alkali soil species
Plant the microbial manure of salt tolerant crop growth.
Document 2 is the patent application of publication number CN201410261688.9, discloses a kind of salt-soda soil growth-promoting biological organic
Fertilizer and preparation method thereof, using bacillus subtilis strain and Li's Trichoderma strains, produces reed wormwood artemisia Straw decomposing microbial inoculum.The microbial inoculum
Can speed up the decomposition rate of reed wormwood artemisia crop straw stubble in reed wormwood artemisia continuous cropping soil, at the same suppress crop straw stubble on pathogenic microorganism it is numerous
Hyperplasia is grown, the harm of reed wormwood artemisia continuous cropping obstacle is reduced.Functional microorganism quantity >=2 × 10 in microbial inoculum8 cfu g-1, water content is less than
15%, the content of organic matter is more than 35%.
Obviously, document 2 provides a kind of salt-soda soil growth-promoting biological organic fertilizer and preparation method thereof, rather than promotes beach saline-alkali soil
The microbial manure of earth plantation salt tolerant crop growth.
Document 3 is publication number CN201410262147.8 patent applications, disclose it is a kind of have prevent and kill off reed wormwood artemisia continuous cropping obstacle
Microbial organic fertilizer of effect and preparation method thereof, it is raw using bacillus subtilis bacteria solid fermentation agricultural organic solid castoff
Product prevents and kill off the microbial organic fertilizer of reed wormwood artemisia continuous cropping obstacle.Functional microorganism quantity >=2 × 10 in the microbial organic fertilizer8 cfu g-1, less than 30%, the content of organic matter is more than 30% to water content.
Obviously, document 3 provides a kind of with microbial organic fertilizer for preventing and kill off reed wormwood artemisia continuous cropping obstacle effect and preparation method thereof,
Rather than promote the microbial manure of beach saline-alkali soil plantation salt tolerant crop growth.
Therefore, a kind of salt-soda soil growth-promoting biological organic fertilizer is developed, serike survival rate and the speed of growth is improved, is to accelerate
The effective way of beach saline-alkali soil improvement.With social development, the attention all the more to environmental protection, using it is nuisanceless, efficient,
Sustainable bio-technology improvement soil suits all the more social demand, therefore, the salt-tolerant plant planted to beach saline-alkali has rush
The biological organic fertilizer of raw effect has very big market prospects.
So far, it is related to the document report of present subject matter without discovery.
The content of the invention
Purpose：In order to overcome the deficiencies in the prior art, the present invention to provide a kind of salt-soda soil growth-promoting biological organic fertilizer,
Biological organic fertilizer utilizes be separated to from beach saline-alkali soil and salination plastic shed soil respectively, spine can promoting plant growth
Spore trichoderma 12 and bacillus subtilis D9, secondary fermentation agricultural solid residue are made, and functional microorganism bacterial strain can in product
Salt affected soil poor environment is adapted to, the effect for promoting growth is played.
Technical scheme：In order to solve the above technical problems, the technical solution adopted by the present invention is：
A kind of salt-soda soil growth-promoting biological organic fertilizer, it is characterised in that：The salt-soda soil growth-promoting biological organic fertilizer is by spine
Spore trichoderma 12 and bacillus subtilis D9 fermentation agricultural solid residues are made.
The trichoderma asperellum 12（Trichoderma asperellum12）, on May 16th, 2013 is preserved in Chinese Typical Representative
Culture collection, preservation address is wuchang, wuhan Luo Jia Shan, and bacterial strain preserving number is CCTCC NO：M2013213.It is described
Bacillus subtilis D9（Bacillus subtilis D9）Chinese microorganism strain preservation pipe is preserved on May 16th, 2014
Reason committee common micro-organisms center, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and bacterial strain preserving number is
CGMCC NO.9170.The Main Biological of bacillus subtilis D9 is：Bacterium colony is white, edge is irregular, dry tack free
Opaque, Gram-positive has motility, shaft-like by electron microscopic observation, raw in gemma, two ends blunt circle.
In the salt-soda soil growth-promoting biological organic fertilizer, quantity >=0.2 × 10 of trichoderma asperellum 128 cfu g-1, bacillus subtilis
Bacterium D9 >=0.5 × 108 cfu g-1, pH value 5.5, water content mass ratio≤30%, quality of organic matter is than content >=45%.
The preparation method of above-mentioned salt-soda soil growth-promoting biological organic fertilizer, it is characterised in that comprise the following steps：
1）Trichoderma asperellum 12 is inoculated into PDA nutrient solutions, liquid fermentation production is carried out, the condition of its fermenting and producing is：Just
Beginning pH scope is 6.5-7.2, and 25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope is 30-100%, 170rpm, later stage bacterium of fermenting
Pompon is all broken into mycelia fragment and obtains the zymotic fluid of trichoderma asperellum 12, the CFU of the zymotic fluid bacterial strain of trichoderma asperellum 12
≥1×108cfu ml-1；
2）By vinegar grain：Wheat bran in mass ratio 5：1 mixing, 120 DEG C of sterilizing 30min are made solid fermentation matrix；By spine spore wood
Mould 12 zymotic fluid is inoculated into solid fermentation matrix, and the inoculum concentration of the zymotic fluid of trichoderma asperellum 12 is the volume of solid fermentation matrix 5%
Than, fermenting 6 days, low temperature drying obtains the solid fermentation strain of trichoderma asperellum 12；
3）Bacillus subtilis D9 is inoculated into steamed beef soup peptone liquid fermentation medium, bacillus subtilis D9 is produced
Zymotic fluid, the condition of its fermenting and producing is：Initial pH scopes are 6.5-7.2,35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope
It is 30-100%, 120-180rpm, ferment 15h, Number of spores >=2 × 10 in bacillus subtilis D9 zymotic fluids8cfu ml-1；
4）By vinegar grain：Wheat bran in mass ratio 3：1 mixing, 120 DEG C of sterilizing 30min, is obtained solid fermentation culture medium；To be obtained
Bacillus subtilis D9 zymotic fluids be inoculated into solid fermentation culture medium according to the ratio of volume ratio 10%, fermentation temperature is 37 DEG C, hair
Ferment incubation time is 5 days, and per stirring once within 10-20 hours in fermentation process, fermentation ends low temperature drying obtains bacillus subtilis
D9 solid fermentation strains；
5）By the solid fermentation strain of trichoderma asperellum 12 and bacillus subtilis D9 solid fermentation strains according to 5:1 mass ratio is mixed
Close, being crushed with pulverizer must mix bacterium powder；
6）Obtained mixing bacterium powder is inoculated into the vinegar grain compost that becomes thoroughly decomposed according to the volume ratio of compost 5-6% or was become thoroughly decomposed
Journey be with the addition of in the compost of cow dung or pig manure, thoroughly stir and evenly mix, and bar buttress formula is banked up fermentation, and fermentation temperature is 30-50 DEG C,
Every turning in 3 days 1 time in fermentation process, fermentation terminates after 15-20 days, and the content of trichoderma asperellum 12 reaches 0.2 × 108cfu g-1With
On, bacillus subtilis D9 contents reach 0.5 × 108cfu g-1More than, bacterial strain stable content, obtaining final product salt-soda soil growth-promoting biology has
Machine fertilizer.
The preparation method of described salt-soda soil growth-promoting biological organic fertilizer, it is characterised in that：Measured with volume 1L, PDA cultures
The compound method of liquid is：It is cut into small pieces to be put into water after being peeled with 100 g potatoes and boils, 30 min are boiled after boiling, filters after filtering
Add 5g common sucroses and 2g rice straw powders in liquid, be settled to 1000 ml, pH value nature, 115 DEG C of 30 min of sterilizing.
The preparation method of described salt-soda soil growth-promoting biological organic fertilizer, it is characterised in that：Measured with volume 1L, steamed beef soup egg
The compound method of peptone liquid fermentation medium is in vain：Beef extract 3g, peptone 10g, NaCl 3g, running water 1000ml, pH scope
7.0-7.5,121 DEG C of sterilizing 20min.
The preparation method of described salt-soda soil growth-promoting biological organic fertilizer, it is characterised in that：Step 6）Compost used, it is organic
Matter quality is than content >=45%, total nutrient >=5%, water content mass ratio≤30%.
Above-mentioned salt-soda soil growth-promoting biological organic fertilizer can be used in the improvement of beach saline-alkali soil.
Beneficial effect：A kind of salt-soda soil growth-promoting biological organic fertilizer that the present invention is provided, by with promotion plant growth
The bacterium of effect and fungus twice fermentation agricultural solid residue production, microbial organic fertilizer of the invention with the market
Product is compared and had the following advantages that：1）Functional microorganism in the biological organic fertilizer is mainly derived from beach geavy salt soil and salt marsh
Change continuous cropping soil, wherein be untreated 2.1 times by the cucumber seeds root length of the fermentation liquor treatment of trichoderma asperellum 12, by withered
The cucumber seeds root length of careless bacillus D9 fermentation liquor treatments is untreated 1.7 times, and two plants of bacterial strains can adapt to high salinity
Environment, is conducive to being colonized in salt affected soil；2）The carrier that the biological organic fertilizer is used is in vinegar grain compost or digest process
The pig manure or cow dung compost of rice husk are added, waits salt affected soil easily hardened due to beach saline-alkali, soil depletion is rotten by heap
The rice husk of process, can effectively be kept soil from packing together, and enough air and heat is provided for growth-promoting microorganism colonizes and grows；3）By
Then, by certain technique productions, growth-promoting functional microorganism can be in soil for biological bacterial strain and cultural solid organic waste
Long-term colonizes, and applied once is permanently effective, without pollution, is conducive to the Sustainable Exploitation of beach saline-alkali soil.
Brief description of the drawings
Fig. 1 is that the effect after the treatment with existing organic fertilizer of the invention compares figure.
Specific embodiment
The present invention is further described with reference to embodiment.
（One）Microbial inoculum is produced
1）Trichoderma asperellum 12 is inoculated into PDA nutrient solutions, liquid fermentation production is carried out, the condition of its fermenting and producing is：Just
Beginning pH scope is 6.5-7.2, and 25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope is 30-100%, 170rpm, later stage bacterium of fermenting
Pompon is all broken into mycelia fragment, CFU >=1 × 10 of zymotic fluid bacterial strain8cfu ml-1；PDA nutrient solutions used
Compound method is（As a example by preparing 1L culture mediums）：It is cut into small pieces to be put into water after being peeled with 100 g potatoes and boils, 30 is boiled after boiling
Min, adds 5g common sucroses and 2g rice straw powders after filtering in filtrate, be settled to 1000 ml, pH value nature, 115 DEG C of sterilizings
30 min。
2）The zymotic fluid of trichoderma asperellum 12 is inoculated into solid fermentation matrix, inoculum concentration is volume ratio 5%, is fermented 6 days, low
Temperature drying；Solid fermentation matrix used is vinegar grain：Wheat bran mass ratio is 5:1,120 DEG C of sterilizing 30min；
3）Bacillus subtilis D9 is inoculated into steamed beef soup peptone liquid fermentation medium, bacillus subtilis D9 is produced
Zymotic fluid, the condition of its fermenting and producing is：Initial pH scopes are 6.5-7.2,35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope
It is 30-100%, 120-180rpm, ferment 15h, Number of spores >=2 × 10 in bacillus subtilis D9 zymotic fluids8cfu ml-1；
The compound method of steamed beef soup peptone liquid fermentation medium used is to be prepared according to 1L culture mediums：Beef extract 3g, peptone
10g, NaCl 3g, running water 1000ml, pH scope 7.0-7.5,121 DEG C of sterilizing 20min；
4）Obtained bacillus subtilis D9 zymotic fluids are inoculated into solid fermentation culture medium according to the ratio of volume ratio 10%,
Fermentation temperature is 37 DEG C, and fermented incubation time is 5 days, and per stirring once within 10-20 hours in fermentation process, fermentation ends obtain withered
Careless bacillus D9 solid fermentation strains, low temperature drying；Solid fermentation culture medium used is vinegar grain：Wheat bran mass ratio is 3:1,
120 DEG C of sterilizing 30min.
5）By the solid fermentation strain of trichoderma asperellum 12 and bacillus subtilis D9 solid fermentation strains according to 5:1 mass Beecher
The mixing at bottom, being crushed with pulverizer must mix bacterium powder.
（Two）Biological organic fertilizer is produced
Mixing bacterium powder is inoculated into the vinegar grain compost for becoming thoroughly decomposed according to the volume ratio of 5%-6%, is thoroughly stirred and evenly mixed, bar pile
Formula is banked up fermentation, and fermentation temperature is 30-50 DEG C, every turning in 3 days 1 time in fermentation process, and fermentation terminates after 15-20 days, and spine spore is wooden
Mould 12 content reaches 0.2 × 108cfu g-1More than, bacillus subtilis D9 contents reach 0.5 × 108cfu g-1More than, bacterial strain
Stable content；The vinegar grain compost for becoming thoroughly decomposed used, quality of organic matter than content >=45%, total nutrient >=5%, water content mass ratio≤
30%.The product of acquisition is growth-promoting biological organic fertilizer in salt-soda soil of the invention.
（Three）Mud-flat soil long-term cropping Biological control
Experiment soil Yancheng, Jiangsu Province Coastal beach soil, soil salt content 25 ‰, pH value 8.39.
Three treatment of Setup Experiments, respectively：1st, control treatment, 2, organic fertilizer treatment, 3, biological organic fertilizer of the invention
Treatment.
Experiment passes through fluffy basin pot culture kind alkali, cyperus malaccensis and summer cypress, soil 5kg is placed per alms bowl, at organic fertilizer and biological organic fertilizer
The rate of fertilizer of reason is respectively the 5% of soil quality（w/w）.Fluffy alkali, cyperus malaccensis and summer cypress are planted in each treatment respectively.Plantation 30
After it, the biomass of the plant of each treatment is determined.
Control treatment alkali is fluffy, cyperus malaccensis and summer cypress survival rate are respectively 80%, 87% and 68%, organic fertilizer process 3 kinds of plants into
Motility rate is respectively 100%, 100% and 85%, and biological organic fertilizer processes 3 kinds of plant survival rates and reaches 100%.The plant of different disposal
Biomass estimation is as shown in figure 1, compared to control treatment, organic fertilizer treatment alkali is fluffy, cyperus malaccensis and summer cypress biomass increased respectively
9.1%th, 15.6% and 11.9%, biological organic fertilizer treatment biomass increased 49.1%, 56.3% and 52.2% respectively.Life of the invention
Organic fertilizer plants surviving and growing for salt-tolerant plant in which can be obviously promoted beach saline-alkali.
The above is only the preferred embodiment of the present invention, it should be pointed out that：For the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (5)
1. a kind of salt-soda soil growth-promoting biological organic fertilizer, it is characterised in that：The salt-soda soil growth-promoting biological organic fertilizer is by spine spore
Trichoderma 12 and bacillus subtilis D9 fermentation agricultural solid residues are made；In the salt-soda soil growth-promoting biological organic fertilizer, spine spore
Quantity >=0.2 × 10 of trichoderma 128 cfu g-1, bacillus subtilis D9 >=0.5 × 108 cfu g-1, pH value 5.5, water content matter
Amount ratio≤30%, quality of organic matter is than content >=45%；
The preparation method of described salt-soda soil growth-promoting biological organic fertilizer, it is characterised in that comprise the following steps：
1）Trichoderma asperellum 12 is inoculated into PDA nutrient solutions, liquid fermentation production is carried out, the condition of its fermenting and producing is：Initial pH
Scope is 6.5-7.2, and 25-30 DEG C of cultivation temperature, dissolved oxygen throughput scope is 30-100%, 170rpm, and ferment later stage mycelium pellet
All being broken into mycelia fragment obtains the zymotic fluid of trichoderma asperellum 12, CFU >=1 of the zymotic fluid bacterial strain of trichoderma asperellum 12
×108cfu ml-1；
2）By vinegar grain：Wheat bran in mass ratio 5：1 mixing, 120 DEG C of sterilizing 30min are made solid fermentation matrix；By trichoderma asperellum 12
Zymotic fluid is inoculated into solid fermentation matrix, and the inoculum concentration of the zymotic fluid of trichoderma asperellum 12 is the volume ratio of solid fermentation matrix 5%,
Fermentation 6 days, low temperature drying obtains the solid fermentation strain of trichoderma asperellum 12；
3）Bacillus subtilis D9 is inoculated into steamed beef soup peptone liquid fermentation medium, bacillus subtilis D9 fermentations are produced
Liquid, the condition of its fermenting and producing is：Initial pH scopes are 6.5-7.2, and 35-37 DEG C of cultivation temperature, dissolved oxygen throughput scope is 30-
100%, 120-180rpm, ferment 15h, Number of spores >=2 × 10 in bacillus subtilis D9 zymotic fluids8cfu ml-1；
4）By vinegar grain：Wheat bran in mass ratio 3：1 mixing, 120 DEG C of sterilizing 30min, is obtained solid fermentation culture medium；Will be obtained withered
Careless bacillus D9 zymotic fluids are inoculated into solid fermentation culture medium according to the ratio of volume ratio 10%, and fermentation temperature is 37 DEG C, fermentation training
The time of supporting is 5 days, and per stirring once within 10-20 hours in fermentation process, fermentation ends low temperature drying obtains bacillus subtilis D9 and consolidates
Body fermented bacterium；
5）By the solid fermentation strain of trichoderma asperellum 12 and bacillus subtilis D9 solid fermentation strains according to 5:1 mass ratio mixes,
Being crushed with pulverizer must mix bacterium powder；
6）Obtained mixing bacterium powder is inoculated into the vinegar grain compost that becomes thoroughly decomposed according to the volume ratio of compost 5-6% or digest process adds
Add in the compost of cow dung or pig manure, thoroughly stirred and evenly mixed, bar buttress formula has been banked up fermentation, fermentation temperature has been 30-50 DEG C, fermentation
During every turning in 3 days 1 time, end after fermentation 15-20 days, the content of trichoderma asperellum 12 reaches 0.2 × 108cfu g-1More than, it is withered
Careless bacillus D9 contents reach 0.5 × 108cfu g-1More than, bacterial strain stable content obtains final product salt-soda soil growth-promoting biological organic fertilizer.
2. growth-promoting biological organic fertilizer in salt-soda soil according to claim 1, it is characterised in that：The trichoderma asperellum 12,2013
On May 16, in is preserved in China typical culture collection center, and preservation address is wuchang, wuhan Luo Jia Shan, and bacterial strain preserving number is
CCTCC NO：M2013213；The bacillus subtilis D9 was preserved in Chinese microorganism strain preservation pipe on May 16th, 2014
Reason committee common micro-organisms center, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and bacterial strain preserving number is
CGMCC NO.9170。
3. growth-promoting biological organic fertilizer in salt-soda soil according to claim 1, it is characterised in that：Measured with volume 1L, PDA cultures
The compound method of liquid is：It is cut into small pieces to be put into water after being peeled with 100 g potatoes and boils, 30 min are boiled after boiling, filters after filtering
Add 5g common sucroses and 2g rice straw powders in liquid, be settled to 1000 ml, pH value nature, 115 DEG C of 30 min of sterilizing.
4. growth-promoting biological organic fertilizer in salt-soda soil according to claim 1, it is characterised in that：Measured with volume 1L, steamed beef soup
The compound method of peptone liquid fermentation medium is：Beef extract 3g, peptone 10g, NaCl 3g, running water 1000ml, pH model
Enclose 7.0-7.5,121 DEG C of sterilizing 20min.
5. growth-promoting biological organic fertilizer in salt-soda soil according to claim 1, it is characterised in that：Step 6）Compost used, has
Machine matter quality is than content >=45%, total nutrient >=5%, water content mass ratio≤30%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510030096.0A CN104609995B (en) | 2015-01-22 | 2015-01-22 | Plant growth promoting bio-organic fertilizer for saline-alkali land |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510030096.0A CN104609995B (en) | 2015-01-22 | 2015-01-22 | Plant growth promoting bio-organic fertilizer for saline-alkali land |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104609995A CN104609995A (en) | 2015-05-13 |
| CN104609995B true CN104609995B (en) | 2017-05-24 |
Family
ID=53144688
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510030096.0A Active CN104609995B (en) | 2015-01-22 | 2015-01-22 | Plant growth promoting bio-organic fertilizer for saline-alkali land |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104609995B (en) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105481486B (en) * | 2016-01-04 | 2019-01-18 | 南京农业大学 | Utilize the product of the method and acquisition of stalk and filter mud production trichoderma as biological organic fertilizer |
| CN105819913A (en) * | 2016-03-14 | 2016-08-03 | 北京天工环境治理有限责任公司 | Composite bacterial fertilizer, preparation method and application thereof |
| CN106190138A (en) * | 2016-06-30 | 2016-12-07 | 山东胜伟园林科技有限公司 | A kind of anti-salt alkali composition containing Semen Strychni and preparation method thereof |
| CN106834143B (en) * | 2017-04-10 | 2020-07-31 | 北京绿安创华环保科技有限公司 | Microbial agent, greening garbage soil remediation agent and application thereof |
| CN106916756B (en) * | 2017-04-10 | 2020-05-19 | 北京绿安创华环保科技有限公司 | Fungus strain, microbial agent, straw soil remediation agent and application thereof |
| CN107746297A (en) * | 2017-10-25 | 2018-03-02 | 山东胜景旅游发展有限公司 | A kind of salt-soda soil Fructus Akebiae plants fertilizer special for organic |
| CN107935672A (en) * | 2017-10-25 | 2018-04-20 | 山东胜景旅游发展有限公司 | A kind of salt-soda soil growth-promoting bio-bacterial manure |
| CN109554312A (en) * | 2018-12-19 | 2019-04-02 | 江苏沿江地区农业科学研究所 | A kind of Facultative Halophiles QM, forest seedling growth matrix and preparation method comprising Facultative Halophiles QM |
| CN109735465A (en) * | 2019-01-25 | 2019-05-10 | 江苏大学 | A kind of improvement beach ground microorganism formulation of plant growth and preparation method thereof |
| CN110603923A (en) * | 2019-09-25 | 2019-12-24 | 正大农业科技(浙江)有限公司 | Biological improvement method for saline-alkali soil |
| CN111406546B (en) * | 2020-03-13 | 2021-03-19 | 河海大学 | Ecological slope protection precast block and preparation method and application thereof |
| CN111647527B (en) * | 2020-05-21 | 2023-01-06 | 浙江工业大学 | Method for improving saline-alkali soil and preserving fertilizer by using bacillus subtilis |
| CN111876342A (en) * | 2020-06-19 | 2020-11-03 | 吉林地富肥业科技有限责任公司 | Low-temperature straw decomposing microbial inoculum and preparation method and application thereof |
| CN112746024A (en) * | 2021-01-19 | 2021-05-04 | 南京农业大学 | Trichoderma composite microbial agent and bio-organic fertilizer prepared from same |
| CN113214034B (en) * | 2021-05-24 | 2023-02-10 | 宁夏润地农牧科技有限公司 | Organic fertilizer applicable to sandy saline-alkali soil and preparation method thereof |
| CN114685201A (en) * | 2022-04-20 | 2022-07-01 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Method for changing substance conversion metabolism and pathogenic microorganism in compost fermentation |
| CN115011353A (en) * | 2022-06-28 | 2022-09-06 | 黑龙江八一农垦大学 | Application of trichoderma asperellum in repairing saline-alkali farmland soil and/or promoting plant growth |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101696390B (en) * | 2009-10-29 | 2010-12-08 | 南京农业大学资产经营有限公司 | Biological preventing and controlling strain of continuous cropping cucumber and watermelon blight and microbe organic fertilizer thereof |
| CN101928182B (en) * | 2010-09-01 | 2013-10-30 | 山西晨雨科技开发连锁经营有限公司 | Bio-organic fertilizer special for improving saline-alkali lands and preparation method thereof |
| CN102533553B (en) * | 2011-12-29 | 2013-06-26 | 湖南省微生物研究所 | Organic matter decomposition combined fungicide for rapidly degrading rice straws and application method thereof |
| CN104177202B (en) * | 2014-07-29 | 2017-05-17 | 天津市农乐制肥有限公司 | Organotrophic saline soil conditioner |
-
2015
- 2015-01-22 CN CN201510030096.0A patent/CN104609995B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104609995A (en) | 2015-05-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104609995B (en) | Plant growth promoting bio-organic fertilizer for saline-alkali land | |
| CN102976801B (en) | Method for producing functional microorganism organic fertilizer by using food residue | |
| CN103113167B (en) | Compound microbial organic fertilizer and preparation method thereof | |
| CN102344812B (en) | Microbiological preparation for improving alkaline land, its preparation method and its application | |
| CN105567612B (en) | A kind of degradation composite bacteria agent preparation of garden waste and application | |
| CN103820349B (en) | One bacillus licheniformis and the production method of microbial inoculum thereof and purposes | |
| CN101792669B (en) | Multi-functional biological agent containing bacillus subtilis | |
| CN101781569B (en) | Soil improvement and anti continuous cropping multifunctional biological agent | |
| CN105753537A (en) | Production method using food residues to prepare functional microorganism organic fertilizer | |
| CN104293694A (en) | Preparation method for sludge aerobic composting composite inoculum | |
| CN103695317B (en) | There is the production method of the efficient phosphate-solubilizing penicillium oxalicum microbial inoculum of heavy metal tolerance characteristic | |
| CN102557837A (en) | Halophilic and alkalophilic microorganism solid bacterial fertilizer of saline-alkaline habitat of desert and preparation method and application thereof | |
| CN103484396B (en) | New strain of streptomyces thermocarboxydus and application thereof | |
| CN102249753A (en) | Method for producing multifunctional bioorganic fertilizer and application of multifunctional bioorganic fertilizer | |
| CN103484410B (en) | Biological agent for compost treatment of livestock manure | |
| CN105695354A (en) | Process and application for treating municipal sewage sludge by means of ultrahigh-temperature aerobic composting fermentation | |
| CN1314325C (en) | Biological prepn. for preventing and controlling soft rot of konjak, and its application | |
| CN104762087B (en) | It is a kind of to be used to improve microorganism additive of beach saline-alkali soil texture and its preparation method and application | |
| CN103805204A (en) | Farmland soil microorganism water-holding product and preparation method thereof | |
| CN103642703B (en) | There is the production method of the efficient phosphate-solubilizing aspergillus japonicus microbial inoculum of heavy metal tolerance characteristic | |
| CN105964680A (en) | Coastal saline continuous cropping cotton soil ecological restoration agent and preparing method and application thereof | |
| CN107840706A (en) | A kind of microbial manure and its application using the production of cassava alcohol waste water | |
| CN102249754A (en) | Paenibacillus polymyxa microbial fertilizer produced through sweet potato starch waste water recovery | |
| CN109320355A (en) | A kind of saliferous bioactivity conditioner of improvement soil in protected field and its application | |
| CN105779323A (en) | Bacillus subtilis capable of efficiently degrading quorum sensing signal molecules and production method of inoculant thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |