CN111876342A - Low-temperature straw decomposing microbial inoculum and preparation method and application thereof - Google Patents
Low-temperature straw decomposing microbial inoculum and preparation method and application thereof Download PDFInfo
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Abstract
The invention is suitable for the technical field of straw returning, and provides a low-temperature straw-decomposing inoculant, a preparation method and an application thereof, wherein the straw-decomposing inoculant comprises a bacillus subtilis culture solution and a trichoderma asperellum culture solution; the bacillus subtilis culture solution is obtained by activating bacillus subtilis and then culturing the activated bacillus subtilis in a culture medium containing corn paste; the culture solution of trichoderma asperellum is obtained by activating trichoderma asperellum and then culturing the activated trichoderma asperellum in a culture medium containing corn paste. The straw decomposition microbial inoculum provided by the invention can play a role in synergy by compounding the bacillus subtilis and the trichoderma asperellum, has activity and degradation rate obviously higher than those of a single strain, has higher capability of degrading cellulase activity and cellulose under a low-temperature condition, and can be used for decomposing straws of corn, rice and the like at a low temperature.
Description
Technical Field
The invention belongs to the technical field of straw returning, and particularly relates to a low-temperature straw decomposing microbial inoculum, and a preparation method and application thereof.
Background
The straw returning is a yield increasing measure for fertilizing soil, which is generally regarded as important at present, and has the functions of increasing fertilizer and increasing yield while avoiding atmospheric pollution caused by straw burning. Particularly, the straw returning can increase soil organic matters, improve the soil structure, loosen the soil, increase the porosity, reduce the capacity and promote the activity of microorganisms and the development of crop roots.
However, due to the fact that the temperature is low when the whole amount of the corn is returned to the field after harvesting in autumn in the north, the corn cannot be well decomposed, and sowing and seedling emergence in spring of the next year can be affected. In addition, the straws are anaerobically decomposed after the full amount of returning to the paddy field and transplanting rice to generate methane and N2Toxic gases such as O and the like cause poor quality of seedlings and dead seedlings in severe cases.
Therefore, at present, a straw decomposition agent which can resist low temperature and has high decomposition rate is urgently needed to be researched so as to solve the problem existing in returning the straws to the field in the north.
Disclosure of Invention
The embodiment of the invention aims to provide a low-temperature straw decomposing inoculant, and aims to solve the problems in the background art.
The embodiment of the invention is realized in such a way that the low-temperature straw decomposing inoculant comprises the following components in parts by weight: 30-70 parts of a bacillus subtilis culture solution and 30-70 parts of a trichoderma asperellum culture solution; the bacillus subtilis culture solution is obtained by activating bacillus subtilis and then culturing the activated bacillus subtilis in a culture medium containing corn paste; the trichoderma asperellum culture solution is obtained by activating trichoderma asperellum and then culturing the activated trichoderma asperellum in a culture medium containing corn paste.
As a preferable scheme of the embodiment of the invention, the straw decomposing inoculant comprises the following components in parts by weight: 40-60 parts of a bacillus subtilis culture solution and 40-60 parts of a trichoderma asperellum culture solution.
As another preferable mode of the embodiment of the present invention, the Trichoderma asperellum is Trichoderma asperellum T-37.
Another object of the embodiment of the present invention is to provide a method for preparing the straw-decomposing inoculant, which comprises the following steps:
activating bacillus subtilis, and then culturing in a bacillus subtilis liquid culture medium added with corn paste to obtain a bacillus subtilis culture solution for later use;
activating the trichoderma asperellum, and then culturing the activated trichoderma asperellum in a trichoderma asperellum liquid culture medium added with corn paste to obtain a trichoderma asperellum culture solution for later use;
weighing a bacillus subtilis culture solution and a trichoderma asperellum culture solution according to the weight parts of the components for later use;
and mixing the weighed bacillus subtilis culture solution and the trichoderma asperellum culture solution to obtain the straw decomposition microbial inoculum.
As another preferable mode of the embodiment of the present invention, the Bacillus subtilis liquid culture medium comprises the following components per liter: 4-6 g of yeast extract, 8-12 g of peptone and 8-12 g of sodium chloride; the trichoderma asperellum liquid culture medium comprises the following components per liter: 1-3 g of sodium carbonate, 0.8-1.2 g of monopotassium phosphate, 0.4-0.6 g of magnesium sulfate, 0.4-0.6 g of potassium chloride, 0.005-0.015 g of ferric sulfate and 15-25 g of glucose .
As another preferred scheme of the embodiment of the present invention, the step of mixing the weighed bacillus subtilis culture solution and trichoderma asperellum culture solution to obtain the straw-decomposing inoculant specifically includes:
mixing the weighed bacillus subtilis culture solution and the trichoderma asperellum culture solution, and adding a compound culture medium for culturing to obtain the straw decomposition microbial inoculum; the composite culture medium comprises the following components in percentage by mass: 53.5 to 84.5 percent of oak sawdust, 10 to 30 percent of production waste of oyster mushroom and/or mushroom, 5 to 15 percent of wheat bran and 0.5 to 1.5 percent of calcium carbonate, wherein the sum of the mass percent of the components is 100 percent.
The embodiment of the invention also aims to provide the straw decomposing inoculant prepared by the preparation method.
As another preferable scheme of the embodiment of the invention, the enzyme activity of the straw-decomposing inoculant at 4-8 ℃ is not lower than 36.85U, and the degradation rate is not lower than 38.83%.
The embodiment of the invention also aims to provide application of the straw decomposing inoculant in straw returning.
As another preferred scheme of the embodiment of the invention, the straw returning method comprises the following steps:
crushing the straws, and uniformly throwing the straws in the field;
spraying the straw decomposing inoculant and urea on the straws in the field, and then deeply turning the straws.
The low-temperature straw-decomposing inoculant provided by the embodiment of the invention can play a role of synergistic interaction by compounding the bacillus subtilis and the trichoderma asperellum, has the activity and degradation rate obviously higher than those of a single strain, has higher cellulase activity and cellulose degradation capacity under the low-temperature condition of 4-8 ℃, can be used for decomposing straws of corn, rice and the like at low temperature, and solves the bottleneck problem of direct full-scale returning of straws to fields under the low-temperature conditions of returning the straws to fields in autumn and spring in the north. Specifically, the embodiment of the invention utilizes the trichoderma asperellum low-temperature enzyme activity to ferment the straws, the temperature near the straws is increased in the fermentation process, then the straws are further decomposed in cooperation with the growth process of the bacillus subtilis, and the temperature can be further increased in the decomposition process so as to ensure the temperature required by the decomposition of the straws under the low-temperature condition, thereby improving the decomposition effect of the straws.
Compared with the prior art, the embodiment of the invention has the following beneficial effects: 1. accelerating the decomposition of the corn or rice straws; 2. improving the organic matter content of the soil; 3. the content of absorbable nutrients in the soil is increased, and the using amount of the fertilizer is reduced; 4. environmental pollution and atmospheric pollution caused by burning the straws are reduced; 5. the production cost of the decomposition agent is reduced; 6. because the low-temperature decomposition capability is improved, the complete returning of the northern straws to the field becomes possible.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
The embodiment provides a low-temperature straw-decomposing inoculant, and the preparation method of the straw-decomposing inoculant comprises the following steps:
s1, taking the two seed tanks, respectively carrying out high-temperature steam sterilization for 0.5 hour, cooling, respectively adding a bacillus subtilis liquid culture medium and a trichoderma asperellum liquid culture medium into the two seed tanks, and carrying out autoclaving. Wherein the temperature of the high-temperature steam is 121 ℃, and the pressure is 0.103 MPa; in addition, the following components are included in each liter of the bacillus subtilis liquid culture medium: yeast extract 4g, peptone 8g, sodium chloride 8 g; the trichoderma asperellum liquid culture medium comprises the following components in each liter: 1g of sodium carbonate, 0.8g of monopotassium phosphate, 0.4g of magnesium sulfate, 0.4g of potassium chloride, 0.005g of ferric sulfate and 15g of glucose .
S2, activating bacillus subtilis at 28 ℃, then placing the activated bacillus subtilis into the bacillus subtilis liquid culture medium, adding corn paste as a culture raw material, and culturing the bacillus subtilis for 36 hours to obtain a bacillus subtilis culture solution for later use; wherein, the Bacillus subtilis can be commercially available common Bacillus subtilis strain.
S3, activating the trichoderma asperellum at 28 ℃, then placing the activated trichoderma asperellum into the trichoderma asperellum liquid culture medium, adding corn paste as a culture raw material, and culturing the trichoderma asperellum for 36 hours to obtain a trichoderma asperellum culture solution for later use; among them, Bacillus subtilis can be selected from Trichoderma asperellum T-37 strain introduced in Russia on the market.
S4, mixing the 3kg of bacillus subtilis culture solution and the 7kg of trichoderma asperellum culture solution to obtain the straw decomposing inoculant.
Example 2
The embodiment provides a low-temperature straw-decomposing inoculant, and the preparation method of the straw-decomposing inoculant comprises the following steps:
s1, taking the two seed tanks, respectively carrying out high-temperature steam sterilization for 1 hour, cooling, respectively adding a bacillus subtilis liquid culture medium and a trichoderma asperellum liquid culture medium into the two seed tanks, and carrying out autoclaving. Wherein the temperature of the high-temperature steam is 125 ℃, and the pressure is 0.168 MPa; in addition, the following components are included in each liter of the bacillus subtilis liquid culture medium: 6g of yeast extract, 12g of peptone and 12g of sodium chloride; the trichoderma asperellum liquid culture medium comprises the following components in each liter: 3g of sodium carbonate, 1.2g of monopotassium phosphate, 0.6g of magnesium sulfate, 0.6g of potassium chloride, 0.015g of ferric sulfate and 25g of glucose .
S2, activating bacillus subtilis at 28 ℃, then placing the activated bacillus subtilis into the bacillus subtilis liquid culture medium, adding corn paste as a culture raw material, and culturing the bacillus subtilis for 36 hours to obtain a bacillus subtilis culture solution for later use; wherein, the Bacillus subtilis can be commercially available common Bacillus subtilis strain.
S3, activating the trichoderma asperellum at 28 ℃, then placing the activated trichoderma asperellum into the trichoderma asperellum liquid culture medium, adding corn paste as a culture raw material, and culturing the trichoderma asperellum for 36 hours to obtain a trichoderma asperellum culture solution for later use; among them, Bacillus subtilis can be selected from Trichoderma asperellum T-37 strain introduced in Russia on the market.
S4, mixing the 7kg of bacillus subtilis culture solution and 3kg of trichoderma asperellum culture solution to obtain the straw decomposing inoculant.
Example 3
The embodiment provides a low-temperature straw-decomposing inoculant, and the preparation method of the straw-decomposing inoculant comprises the following steps:
s1, taking the two seed tanks, respectively carrying out high-temperature steam sterilization for 0.8 hour, cooling, respectively adding a bacillus subtilis liquid culture medium and a trichoderma asperellum liquid culture medium into the two seed tanks, and carrying out autoclaving. Wherein the temperature of the high-temperature steam is 123 ℃, and the pressure is 0.13 MPa; in addition, the following components are included in each liter of the bacillus subtilis liquid culture medium: 5g of yeast extract, 10g of peptone and 10g of sodium chloride; the trichoderma asperellum liquid culture medium comprises the following components in each liter: 2g of sodium carbonate, 1g of monopotassium phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 0.01g of ferric sulfate and 20g of glucose .
S2, activating bacillus subtilis at 28 ℃, then placing the activated bacillus subtilis into the bacillus subtilis liquid culture medium, adding corn paste as a culture raw material, and culturing the bacillus subtilis for 36 hours to obtain a bacillus subtilis culture solution for later use; wherein, the Bacillus subtilis can be commercially available common Bacillus subtilis strain.
S3, activating the trichoderma asperellum at 28 ℃, then placing the activated trichoderma asperellum into the trichoderma asperellum liquid culture medium, adding corn paste as a culture raw material, and culturing the trichoderma asperellum for 36 hours to obtain a trichoderma asperellum culture solution for later use; among them, Bacillus subtilis can be selected from Trichoderma asperellum T-37 strain introduced in Russia on the market.
S4, mixing the 4kg of bacillus subtilis culture solution and the 6kg of trichoderma asperellum culture solution at the temperature of 24 ℃, adding a compound culture medium to culture for 14 days, and preparing a solid biological preparation to obtain a straw decomposition microbial inoculum; wherein the mass content of each component in the composite culture medium is as follows: 84.5 percent of oak sawdust, 10 percent of oyster mushroom production waste, 5 percent of wheat bran and 0.5 percent of calcium carbonate.
Example 4
The embodiment provides a low-temperature straw-decomposing inoculant, and the preparation method of the straw-decomposing inoculant comprises the following steps:
s1, taking the two seed tanks, respectively carrying out high-temperature steam sterilization for 0.8 hour, cooling, respectively adding a bacillus subtilis liquid culture medium and a trichoderma asperellum liquid culture medium into the two seed tanks, and carrying out autoclaving. Wherein the temperature of the high-temperature steam is 122 ℃, and the pressure is 0.14 MPa; in addition, the following components are included in each liter of the bacillus subtilis liquid culture medium: 5g of yeast extract, 10g of peptone and 10g of sodium chloride; the trichoderma asperellum liquid culture medium comprises the following components in each liter: 2g of sodium carbonate, 1g of monopotassium phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 0.01g of ferric sulfate and 20g of glucose .
S2, activating bacillus subtilis at 28 ℃, then placing the activated bacillus subtilis into the bacillus subtilis liquid culture medium, adding corn paste as a culture raw material, and culturing the bacillus subtilis for 36 hours to obtain a bacillus subtilis culture solution for later use; wherein, the Bacillus subtilis can be commercially available common Bacillus subtilis strain.
S3, activating the trichoderma asperellum at 28 ℃, then placing the activated trichoderma asperellum into the trichoderma asperellum liquid culture medium, adding corn paste as a culture raw material, and culturing the trichoderma asperellum for 36 hours to obtain a trichoderma asperellum culture solution for later use; among them, Bacillus subtilis can be selected from Trichoderma asperellum T-37 strain introduced in Russia on the market.
S4, mixing the 6kg of bacillus subtilis culture solution and the 4kg of trichoderma asperellum culture solution at 26 ℃, adding a compound culture medium to culture for 14 days, and preparing a solid biological preparation to obtain a straw decomposition microbial inoculum; wherein the mass content of each component in the composite culture medium is as follows: 53.5 percent of oak sawdust, 30 percent of mushroom production waste, 15 percent of wheat bran and 1.5 percent of calcium carbonate.
Example 5
The embodiment provides a low-temperature straw-decomposing inoculant, and the preparation method of the straw-decomposing inoculant comprises the following steps:
s1, taking the two seed tanks, respectively carrying out high-temperature steam sterilization for 0.8 hour, cooling, respectively adding a bacillus subtilis liquid culture medium and a trichoderma asperellum liquid culture medium into the two seed tanks, and carrying out autoclaving. Wherein the temperature of the high-temperature steam is 123 ℃, and the pressure is 0.135 MPa; in addition, the following components are included in each liter of the bacillus subtilis liquid culture medium: 5g of yeast extract, 10g of peptone and 10g of sodium chloride; the trichoderma asperellum liquid culture medium comprises the following components in each liter: 2g of sodium carbonate, 1g of monopotassium phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 0.01g of ferric sulfate and 20g of glucose .
S2, activating bacillus subtilis at 28 ℃, then placing the activated bacillus subtilis into the bacillus subtilis liquid culture medium, adding corn paste as a culture raw material, and culturing the bacillus subtilis for 36 hours to obtain a bacillus subtilis culture solution for later use; wherein, the Bacillus subtilis can be commercially available common Bacillus subtilis strain.
S3, activating the trichoderma asperellum at 28 ℃, then placing the activated trichoderma asperellum into the trichoderma asperellum liquid culture medium, adding corn paste as a culture raw material, and culturing the trichoderma asperellum for 36 hours to obtain a trichoderma asperellum culture solution for later use; among them, Bacillus subtilis can be selected from Trichoderma asperellum T-37 strain introduced in Russia on the market.
S4, mixing the 5kg of bacillus subtilis culture solution and the 5kg of trichoderma asperellum culture solution at the temperature of 25 ℃, adding a compound culture medium to culture for 14 days, and preparing a solid biological preparation to obtain a straw decomposition microbial inoculum; wherein the mass content of each component in the composite culture medium is as follows: 69% of oak sawdust, 20% of production waste of oyster mushrooms and lentinus edodes, 10% of wheat bran and 1% of calcium carbonate.
The enzyme activity and the degradation rate of the bacillus subtilis culture solution, the trichoderma asperellum culture solution and the straw decomposition microbial inoculum obtained in the example 5 at 4-8 ℃ are respectively tested, and the test results are shown in table 1.
TABLE 1
| Example 5 | Enzyme activity/U | Percent of degradation/%) |
| Bacillus subtilis culture solution | 29.18 | 25.38 |
| Trichoderma asperellum culture solution | 34.82 | 28.17 |
| Straw decomposition bacterial agent | 36.85 | 38.83 |
As can be seen from Table 1, the straw-decomposing inoculant obtained in the embodiment of the invention can play a role of synergy by compounding the bacillus subtilis and the trichoderma asperellum, and the activity and the degradation rate of the straw-decomposing inoculant are obviously higher than those of a single strain.
In addition, the corn straws are directly returned to the field in full, and the straw decomposition microbial inoculum provided by the embodiment 5 can effectively decompose the straws, so that the sowing quality is improved, the emergence rate is improved by more than 5%, and the yield is increased by more than 8%; after the seedlings are transplanted, the anaerobic fermentation is reduced by more than 50%, the toxic gas is reduced by more than 50%, the seedling quality is effectively improved, the tillering number, the plant height and the color of the seedlings are effectively improved, the yield and character indexes such as spike grain number, hundred grain weight and the like are obviously increased, and the yield is increased by more than 10% compared with the traditional straw returning technology.
The first test example:
the straw-decomposing inoculant provided by the embodiment 5 is used for a test in a straw return field of a northern rice field, and is used as a test group I, and the straw return field is used without adding the straw-decomposing inoculant as a control group I.
Specifically, the method for returning rice straws to the field of the test group I comprises the following steps: 1. when the rice is harvested, the straws are crushed and uniformly thrown in the field, and the crop straws are less than 5 cm. 2. Ploughing or rotary tillage: 2 kg/mu of the straw-decomposing inoculant and 5 kg/mu of urea provided by the embodiment 5 are sprayed on the straws in autumn, and the decomposing inoculant and the urea are uniformly distributed in the field by turning over or rotary tillage; irrigating and soaking the rice field in the spring of the 2 nd year to reach the state of waiting for transplanting.
The rice yield and soil nutrients before and after returning the straws to the field of the test group I and the control group are measured, and the measurement results are respectively shown in tables 2 and 3.
TABLE 2
TABLE 3
Test example two:
the straw-decomposing inoculant provided in the example 5 is subjected to a 5 hectare corn straw full-depth turning returning test in the Jilin Jizhuging city towards the sunny slope town as a second test group, and the straw is returned to the field without adding the straw-decomposing inoculant as a second control group.
Specifically, the corn stalk returning method of the test group two is as follows: 1. harvesting: harvesting seeds by a large-scale machine in autumn, simultaneously carrying out straw crushing operation and uniformly throwing the crushed straws in the field, wherein the crop straws are less than 5 cm; 2. 2 kg/mu of straw decomposition microbial inoculum and 5 kg/mu of urea provided by the embodiment 5 are sprayed; 3. the straws are deeply ploughed to a soil layer of 20-30 cm by a hydraulic amplitude modulation turnover plow with power of more than 140 horsepower, and soil preparation is carried out by a light rake or rotary tillage in combination with local soil conditions to reach a state of waiting for sowing.
The corn yield and soil nutrients before and after straw returning of the test group II and the control group II are measured, and the measurement results are respectively shown in tables 4 and 5.
TABLE 4
TABLE 5
As can be seen from tables 2-5, when the straw is returned to the field, the straw decomposition rate can be obviously improved by using the straw decomposition microbial inoculum provided by the embodiment of the invention, so that various indexes such as seedling tiller number, plant height, kernel number, yield and the like are obviously improved, and the nutrient content of soil can be obviously improved.
Test example three:
the average growth rates of different Trichoderma asperellum strains (strain numbers T-1, T-5, T-9, T-28, T-37 and T-49 respectively) were tested at 4-8 ℃, and the test results are shown in Table 6.
TABLE 6
As can be seen from the above table 6, the Trichoderma asperellum T-37 strain has a good linear growth rate at a low temperature of 4-8 ℃, so the Trichoderma asperellum T-37 strain is preferably used as a raw material of the straw decomposition microbial inoculum.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. The low-temperature straw decomposing inoculant is characterized by comprising the following components in parts by weight: 30-70 parts of a bacillus subtilis culture solution and 30-70 parts of a trichoderma asperellum culture solution; the bacillus subtilis culture solution is obtained by activating bacillus subtilis and then culturing the activated bacillus subtilis in a culture medium containing corn paste; the trichoderma asperellum culture solution is obtained by activating trichoderma asperellum and then culturing the activated trichoderma asperellum in a culture medium containing corn paste.
2. The low-temperature straw-decomposing inoculant according to claim 1, wherein the straw-decomposing inoculant comprises the following components in parts by weight: 40-60 parts of a bacillus subtilis culture solution and 40-60 parts of a trichoderma asperellum culture solution.
3. The low-temperature straw-decomposing inoculant according to claim 1 or 2, wherein the Trichoderma asperellum is Trichoderma asperellum T-37.
4. A preparation method of the straw-decomposing inoculant according to any one of claims 1 to 3, comprising the following steps:
activating bacillus subtilis, and then culturing in a bacillus subtilis liquid culture medium added with corn paste to obtain a bacillus subtilis culture solution for later use;
activating the trichoderma asperellum, and then culturing the activated trichoderma asperellum in a trichoderma asperellum liquid culture medium added with corn paste to obtain a trichoderma asperellum culture solution for later use;
weighing a bacillus subtilis culture solution and a trichoderma asperellum culture solution according to the weight parts of the components for later use;
and mixing the weighed bacillus subtilis culture solution and the trichoderma asperellum culture solution to obtain the straw decomposition microbial inoculum.
5. The method for preparing a straw-decomposing inoculant according to claim 4, wherein each liter of the liquid culture medium of the bacillus subtilis comprises the following components: 4-6 g of yeast extract, 8-12 g of peptone and 8-12 g of sodium chloride; the trichoderma asperellum liquid culture medium comprises the following components per liter: 1-3 g of sodium carbonate, 0.8-1.2 g of monopotassium phosphate, 0.4-0.6 g of magnesium sulfate, 0.4-0.6 g of potassium chloride, 0.005-0.015 g of ferric sulfate and 15-25 g of glucose .
6. The method for preparing a straw-decomposing inoculant according to claim 4, wherein the step of mixing the weighed bacillus subtilis culture solution and trichoderma asperellum culture solution to obtain the straw-decomposing inoculant specifically comprises:
mixing the weighed bacillus subtilis culture solution and the trichoderma asperellum culture solution, and adding a compound culture medium for culturing to obtain the straw decomposition microbial inoculum; the composite culture medium comprises the following components in percentage by mass: 53.5 to 84.5 percent of oak sawdust, 10 to 30 percent of production waste of oyster mushroom and/or mushroom, 5 to 15 percent of wheat bran and 0.5 to 1.5 percent of calcium carbonate, wherein the sum of the mass percent of the components is 100 percent.
7. A straw-decomposing inoculant prepared by the preparation method of any one of claims 4 to 6.
8. The straw decomposing inoculant according to claim 7, wherein the enzyme activity of the straw decomposing inoculant at 4-8 ℃ is not lower than 36.85U, and the degradation rate is not lower than 38.83%.
9. Use of the straw-decomposing inoculant according to any one of claims 1-3 and 7-8 in straw reduction.
10. The application of claim 9, wherein the straw returning method comprises the following steps:
crushing the straws, and uniformly throwing the straws in the field;
spraying the straw decomposing inoculant and urea on the straws in the field, and then deeply turning the straws.
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| CN114262672A (en) * | 2021-11-29 | 2022-04-01 | 吉林农业大学 | Composite microbial inoculum for low-temperature degradation of corn straws |
| CN114657094A (en) * | 2022-03-14 | 2022-06-24 | 河北新世纪周天生物科技有限公司 | High-efficiency straw decomposition agent suitable for low-temperature environment |
| CN116924839A (en) * | 2023-07-25 | 2023-10-24 | 江苏省农业科学院 | Bacillus subtilis is used for promoting crop straw decomposition and reducing soil N 2 Application in O emission |
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| CN114657094A (en) * | 2022-03-14 | 2022-06-24 | 河北新世纪周天生物科技有限公司 | High-efficiency straw decomposition agent suitable for low-temperature environment |
| CN116924839A (en) * | 2023-07-25 | 2023-10-24 | 江苏省农业科学院 | Bacillus subtilis is used for promoting crop straw decomposition and reducing soil N 2 Application in O emission |
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