CN102382768A - Microbial straw decomposition additive as well as preparation method and application thereof - Google Patents

Microbial straw decomposition additive as well as preparation method and application thereof Download PDF

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Publication number
CN102382768A
CN102382768A CN2011102909848A CN201110290984A CN102382768A CN 102382768 A CN102382768 A CN 102382768A CN 2011102909848 A CN2011102909848 A CN 2011102909848A CN 201110290984 A CN201110290984 A CN 201110290984A CN 102382768 A CN102382768 A CN 102382768A
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China
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microbial
thoroughly decomposed
straw
agent
becoming thoroughly
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刘全礼
谷序文
叶劲松
黄海英
曹芬
蔡君
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WUHAN THRIVE BIOLOGICAL TECHNOLOGY Co Ltd
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WUHAN THRIVE BIOLOGICAL TECHNOLOGY Co Ltd
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Abstract

The invention discloses microbial straw decomposition additive, which belongs to the field of straw decomposition additive and is obtained through combining four bacteria: bacillus subtilis, aspergillus niger, trichoderma reesei and saccharomyces cerevisiae. The microbial straw decomposition additive utilizes the temperature rise benefit of the aspergillus niger spore enzyme activity for raising the environment temperature of decomposition, the further temperature rise benefit in the growth process of the bacillus subtilis is cooperated, and the temperature required by the decomposition is ensured, so the decomposition effect of straws is improved. The microbial straw decomposition additive has the obvious temperature rise benefit and can be used for the in-site field returning decomposition of the straws, the straw decomposition can be better accelerated in low-temperature and normal-temperature environment, the bottleneck problem of straw utilization is solved, the straw burning can be effectively reduced, the decomposition work procedure and the labor cost are saved, the fertilizer feeding is reduced, the soil structure is improved, and the planting benefit is improved.

Description

The microbial straw agent of becoming thoroughly decomposed
Technical field
The present invention relates to the agent of becoming thoroughly decomposed of a kind of stalk, relate in particular to a kind of microorganism-decomposing agent of quick composting stalk, the invention still further relates to the preparation method and the application of this microorganism-decomposing agent, belong to the stalk agent field of becoming thoroughly decomposed.
Background technology
Research shows, problems such as the serious day by day arable soil of China hardens, soil fertility decline are because soil organic matter content is low excessively, uses chemical fertilizer in a large number and fewly causes with fertilizer.The means of employing straw-returning can effectively improve the content of the soil organism; Thereby improve the physico-chemical property of soil; But the direct returning to farmland of excessive stalk has caused agriculture safety and environmental problem again with burning; So the agent of becoming thoroughly decomposed of exploitation biological straw is used for straw-returning and has very important realistic meaning.
There is the defective of the stalk that heats up slowly, is difficult to become thoroughly decomposed rapidly mostly in the agent of becoming thoroughly decomposed of prior biological stalk, haves much room for improvement.
Summary of the invention
Technical problem to be solved by this invention be overcome the prior biological stalk become thoroughly decomposed the existing intensification of agent slowly, the defective of the stalk that is difficult to become thoroughly decomposed rapidly; The agent of becoming thoroughly decomposed of a kind of new microbial straw is provided, this microbial straw advantages such as agent has quick heating, the stalk that can become thoroughly decomposed rapidly, completely of becoming thoroughly decomposed.
Technical problem to be solved by this invention realizes through following technical scheme:
The agent of becoming thoroughly decomposed of a kind of microbial straw, composed of the following components: subtilis (Bacillus subtilis) microbial inoculum, black mold (Aspergillus niger) microbial inoculum, Trichodermareesei (Trichoderma reesei) microbial inoculum, S. cervisiae (Saccharomyces cerevisiae) microbial inoculum.
The present invention is directed to current biological straw become thoroughly decomposed agent when being applied to straw-returning, heat up under the existing coldcondition difficult, become thoroughly decomposed and wait problem slowly; Through a large amount of microbial strainss is screened; The final discovery; With the resultant together microbial preparation of black mold microbial inoculum, yeast saccharomyces cerevisiae bacteria agent, Trichodermareesei microbial inoculum and bacillus subtilis microbial agent compatibility; Four kinds of function yeast are combined has synergistic function, can promote stalk to become thoroughly decomposed rapidly and go back the field, and the additional soil organism, the abundant n p k nutrition that utilizes wherein that discharges improve the soil microorganisms environment simultaneously.Black mold (Aspergillus niger) is the powerful decomposer of occurring in nature " three elements "; Tool higher " three plain enzymes " enzyme is lived; Its a large amount of spores can produce heat sprouting rapidly more than 0 ℃, improve envrionment temperature, the temperature condition of necessity are provided for the quick breeding of self and other function yeast; Trichodermareesei (Trichoderma reesei) has outstanding cellulase activity, is promoting with the Mierocrystalline cellulose to be that main becoming thoroughly decomposed of stalk has obvious effect.Subtilis (Bacillus subtilis) has strong resolving power to " three elements "; Tool higher " three plain enzymes " enzyme is lived, and sprouts rapidly by black mold intensification effect, brings persistently overheating effect to environment; Further improve enzyme and live, quicken straw and become thoroughly decomposed; And yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) can decompose the meta-bolites that utilizes the first three function yeast; Eliminate their growth-inhibiting to the former; Promote the growth and breeding of other indigenous microorganism simultaneously, quicken the speed of becoming thoroughly decomposed and improve the soil microorganisms environment.
The present invention finds through rotten indexs such as the effect that relatively heats up, cellulase activity and humic acids content; Just black mold microbial inoculum, yeast saccharomyces cerevisiae bacteria agent, Trichodermareesei microbial inoculum and bacillus subtilis microbial agent carry out compatibility according to different mass ratios, are promoting there is significant difference on the technique effect that stalk becomes thoroughly decomposed.The present invention finds through a large amount of shaker tests, four kinds of microbial inoculums is carried out compatibility, that is: bacillus subtilis microbial agent according to following mass ratio: the black mold microbial inoculum: the Trichodermareesei microbial inoculum: yeast saccharomyces cerevisiae microbial inoculum=2-4: 10-3: 2-4: 0.5-1.5; Preferred, bacillus subtilis microbial agent: black mold microbial inoculum: Trichodermareesei microbial inoculum: yeast saccharomyces cerevisiae microbial inoculum=3: 2: 3: 1; Become thoroughly decomposed effect and the intensification benefit of resulting microbial preparation are the most remarkable; In 4 ℃ of left and right sides environment; The stalk temperature reaches more than 15 ℃ after becoming thoroughly decomposed 3 days; About 18 days wheat, paddy rice, rape are gone back the field stalk on the spot and become thoroughly decomposed fully, in the environment, promptly reach the effect of well becoming thoroughly decomposed about 10 days more than 20 ℃.
Another technical problem to be solved by this invention provides a kind of become thoroughly decomposed method of agent of mentioned microorganism stalk for preparing;
Another technical problem to be solved by this invention realizes through following technical scheme:
A kind of become thoroughly decomposed method of agent of mentioned microorganism stalk for preparing may further comprise the steps:
(1) prepares subtilis (Bacillus subtilis) microbial inoculum, black mold (Aspergillus niger) microbial inoculum, Trichodermareesei (Trichoderma reesei) microbial inoculum, S. cervisiae (Saccharomyces cerevisiae) microbial inoculum respectively;
(2) with after four kinds of prepared microbial inoculum drying and crushing, mix, promptly get.
Subtilis of the present invention (Bacillus subtilis), black mold (Aspergillus niger), Trichodermareesei (Trichoderma reesei) and S. cervisiae (Saccharomyces cerevisiae) all can be bought through commercial sources and obtain; The composition and the fermentation culture method of the substratum that is improved according to bacterial classification supplier, those skilled in the art can prepare the solid fungicide of above-mentioned four kinds of bacterial classifications.As a reference, the substratum of the used four kinds of bacterium of the present invention composition and fermentation culture conditions are following:
The liquid nutrient medium of four kinds of bacterium is PDA SM (20% yam+2% sucrose).Wherein, liquid of subtilis and solid fermentation temperature are 37 ℃, and solid medium consists of: wheat bran is 30%, and rice bran is 68%, and inorganic salt comprise sucrose 1%, ammonium sulfate 0.5%, unslaked lime 0.5%; Material-water ratio is 1: 0.8 before the inoculation.
The solid of Trichodermareesei and black mold and liquid fermenting temperature are 28 ℃, and solid medium consists of: wheat bran 50%, rice bran 49%, ammonium sulfate and potassium primary phosphate each 0.5%; Material-water ratio is 1: 0.6 before the inoculation;
Yeast saccharomyces cerevisiae solid and liquid fermenting temperature are 30 ℃, and the solid culture based formulas is a wheat bran 80%, and rice bran 20% does not add inorganic salt.
The present invention is prepared to be obtained the become thoroughly decomposed viable count of agent product of microbial straw and reaches 100,000,000 CFU/ gram; Cellulase activity reaches the 1000U/ gram, and lignoenzyme work reaches 500U/ gram (annotating: per hour generate the required enzyme amount of 1mmol glucose by substrate and be defined as an enzyme activity unit (U)).
The become thoroughly decomposed method of use of agent of microbial straw of the present invention:
1, concentrate the stack retting method of becoming thoroughly decomposed: the agricultural crop straw that canes such as corn, Chinese sorghum is more thick and stiff pulverize or be cut into kibbler 3-5 centimetre subsequent use, wheat straw, straw, leaf, weeds, peanut seedling, beanstalk etc. can directly be used for fermentation.Add the agent of becoming thoroughly decomposed of 0.5-1.0 kilogram microbial straw of the present invention by tangerine bar per ton (doing), water waters the tangerine bar then, and diammonium phosphate (with the stalk dry weight basis) or the ight soil water and the stalk that add 2-5% when watering mix, and the stalk water cut is grasped at 60-70%.Play the heap back with plastics film abdomen lid, divide evaporation, the diffusion of heap temperature and nutrient loss to prevent water.Generally, can become thoroughly decomposed in stalk 15-20 days, overstrike or chocolate are at organic fertilizer.
2, tangerine bar direct returning to farmland quick composting method: after agricultural crop straw (wheat, paddy rice, corn etc.) is scattered in the field; Convert water 30-50 kilogram by 5 kilograms in every mu of 2 kilograms+urea and evenly spray with the microbial straw of the present invention agent of becoming thoroughly decomposed on stalk surface; To regulate the stalk C/N ratio; Then with agricultural crop straw and microbial straw of the present invention become thoroughly decomposed agent together plough under underground, the degree of depth about 10-15 centimetre, make divide stalk to imbed in the soil extremely greatly after; Keep soil humidity at 50-60%, can reach the effect of becoming thoroughly decomposed in 20-30 days.
Suitable object: do, stalk of fresh corn, straw, rice straw, paddy stalk, Ipomoea batatas rattan, broad bean straw, rape stalk, weeds, leaf, domestic refuse that fibrous matter content is high etc.
The scope of application: the tangerine bar is the field fast rotting also, and handle the early stage of auxiliary materials such as peasant household's compost, making seedling medium, fertilizer factory stalk.
The present invention is directed to the existing stalk agent problem that existing intensification is slow, difficulty is become thoroughly decomposed when becoming thoroughly decomposed field stalk also of becoming thoroughly decomposed,, at first carry out single bacterial strain experiment according to the characteristic of selected function bacterial classification; Investigate under the low temperature of each single bacterium the intensification ability with become thoroughly decomposed ability, four kinds of bacterium are made up the intensification of more various combinations and the ability of becoming thoroughly decomposed according to the function of each bacterium; Obtain best of breed; Then consumption is optimized, then the products production zymotechnique is optimized, carry out field test and field experiment at last; The agent of becoming thoroughly decomposed of the high biological straw of safe, efficient and simple suitability price ratio has been developed in checking product application effect and security.
The present invention makes up subtilis, black mold, Trichodermareesei, four kinds of bacterium of yeast saccharomyces cerevisiae and obtains the stalk agent of becoming thoroughly decomposed; Make full use of aspergillus niger spore enzyme intensification benefit alive and improve the envrionment temperature of becoming thoroughly decomposed; The further intensification benefit of collaborative subtilis process of growth; Assurance is become thoroughly decomposed temperature required, solves the current stalk agent difficult subject matter that heats up of becoming thoroughly decomposed.The present invention utilizes Mierocrystalline cellulose and the wooden mould enzyme active benefit of subtilis, black mold, Trichodermareesei three strain bacterium, the promotes growth benefit of auxiliary yeast saccharomyces cerevisiae, and reasonable combination improves the effect of becoming thoroughly decomposed.
The microbial straw of the present invention agent intensification benefit of becoming thoroughly decomposed is obvious; Can be used for stalk on the spot and become thoroughly decomposed in the field, practiced thrift become thoroughly decomposed operation and labor cost greatly, solved the bottleneck problem of straw utilization; Under low temperature and normal temperature environment, all can quicken stalk well becomes thoroughly decomposed; Can effectively reduce crop straw burning, chemical fertilizer input, the structure of improving the soil improves planting benefit.
Description of drawings
Become thoroughly decomposed agent preparing method's process route chart of Fig. 1 microbial straw of the present invention.
Embodiment
Further describe the present invention below in conjunction with specific embodiment, advantage of the present invention and characteristics will be more clear along with description.But these embodiment only are exemplary, scope of the present invention are not constituted any restriction.It will be understood by those skilled in the art that and down can make amendment with form or replace without departing from the spirit and scope of the present invention, but these modifications and replacing all fall in protection scope of the present invention the details of technical scheme of the present invention.
Test materials
Used four bacterial classifications of the present invention are all purchased in China Committee for Culture Collection of Microorganisms agricultural mikrobe center; Wherein the commodity purchasing of subtilis is numbered: ACCC 02973, and the goods number of black mold is: ACCC 31494, and the goods number of Trichodermareesei is: ACCC 30912, and the goods number of yeast saccharomyces cerevisiae is: ACCC 20064.
The preparation of preparation embodiment 1 subtilis solid fungicide
1, Bacillus subtilis strain is inoculated in the substratum (the formulated substratum that provides by supplier) cultivates; With storing in 4 ℃ of refrigerators of medium slant seed bacterial strain; Take out activation twice before using, the bacterial strain after the activation is changeed 250 milliliters of eggplant bottle nutrient agar mediums cultivate, culture temperature is 37 ℃; Wash with aqua sterilisa during 48 hours well-growns, as the inoculum of seeding tank;
2, in 1 ton fermentor tank, add substratum, it consists of: wheat bran is 30%, and rice bran is 68%, and inorganic salt comprise sucrose 1%, ammonium sulfate 0.5%, unslaked lime 0.5%; Initial pH7.5; Material-water ratio is 1: 0.8 before the inoculation.To producing a jar inoculation, begin fermentation from seeding tank.Fermentation condition: culture temperature is 37 ℃, and ventilation is 1: 1.1, and tank pressure is 0.05mpa/cm 2, stirring velocity is 220rpm, inoculates after 20 hours and whenever takes a sample once at a distance from 4 hours; Observe the thalli growth situation, whether pollution arranged, wait to find spore generate reach 90% or more after, the liquid fermenting completion; In zymocyte liquid, add conventional sorbent material and weighting agent, get bacillus subtilis microbial agent.
The preparation of preparation embodiment 2 black mold solid fungicides
1, aspergillus niger strain is inoculated in the substratum (the formulated substratum that provides by supplier) cultivates; With storing in 4 ℃ of refrigerators of medium slant seed bacterial strain; Take out activation twice before using, the bacterial strain after the activation is changeed 250 milliliters of eggplant bottle nutrient agar mediums cultivate, culture temperature is 28 ℃; Wash with aqua sterilisa during 48 hours well-growns, as the inoculum of seeding tank;
2, in 1 ton fermentor tank, add substratum, it consists of: wheat bran 50%, rice bran 49%, ammonium sulfate and potassium primary phosphate each 0.5%; Initial pH7.5; Material-water ratio is 1: 0.6 before the inoculation.To producing a jar inoculation, begin fermentation from seeding tank.Fermentation condition: culture temperature is 28 ℃, and ventilation is 1: 1.1, and tank pressure is 0.05mpa/cm 2, stirring velocity is 220rpm, inoculates after 20 hours and whenever takes a sample once at a distance from 4 hours; Observe the thalli growth situation, whether pollution arranged, wait to find spore generate reach 90% or more after, the liquid fermenting completion; In zymocyte liquid, add conventional sorbent material and weighting agent, get the black mold microbial inoculum.
The preparation of preparation embodiment 3 Trichodermareesei solid fungicides
1, the Trichodermareesei bacterial classification is inoculated in the substratum (the formulated substratum that provides by supplier) cultivates; With storing in 4 ℃ of refrigerators of medium slant seed bacterial strain; Take out activation twice before using, the bacterial strain after the activation is changeed 250 milliliters of eggplant bottle nutrient agar mediums cultivate, culture temperature is 28 ℃; Wash with aqua sterilisa during 48 hours well-growns, as the inoculum of seeding tank;
2, in 1 ton fermentor tank, add substratum, it consists of: wheat bran 50%, rice bran 49%, ammonium sulfate and potassium primary phosphate each 0.5%; Initial pH7.5; Material-water ratio is 1: 0.6 before the inoculation.To producing a jar inoculation, begin fermentation from seeding tank.Fermentation condition: culture temperature is 28 ℃, and ventilation is 1: 1.1, and tank pressure is 0.05mpa/cm 2, stirring velocity is 220rpm, inoculates after 20 hours and whenever takes a sample once at a distance from 4 hours; Observe the thalli growth situation, whether pollution arranged, wait to find spore generate reach 90% or more after, the liquid fermenting completion; In zymocyte liquid, add conventional sorbent material and weighting agent, get the Trichodermareesei microbial inoculum.
The preparation of preparation embodiment 4 yeast saccharomyces cerevisiae solid fungicides
1, the S. cervisiae bacterial classification inoculation is cultivated in substratum (the formulated substratum that provides by supplier); Medium slant seed bacterial strain added behind the MO store in 4 ℃ of refrigerators, take out activation twice before using, the bacterial strain after the activation is changed in 250 milliliters of eggplant bottles cultivate; Culture temperature is 30 ℃; Cultivated 48 hours, and with sterile saline it was washed when strain growth is good, eluate is the inoculum of seeding tank.
2, in 1 ton fermentor tank, add substratum: wheat bran 80%, rice bran 20% does not add inorganic salt.To producing a jar inoculation, inoculum size is 50kg, begins fermentation from seeding tank.Fermentation condition: culture temperature is 30 ℃, and tank pressure is 0.05mpa/cm 2, stirring velocity is 220rpm, ventilating ratio 1: 1.1; Inoculate after 20 hours and whenever took a sample once, observe the thalli growth situation, whether pollution is arranged, after liquid fermenting is accomplished at a distance from 4 hours; In zymocyte liquid, add conventional sorbent material and weighting agent XG 550, get the yeast saccharomyces cerevisiae bacteria agent.
The become thoroughly decomposed preparation of agent of embodiment 1 microbial straw of the present invention
1, takes by weighing each microbial inoculum by following weight: bacillus subtilis microbial agent 3kg, black mold microbial inoculum 2kg, Trichodermareesei microbial inoculum 3kg, yeast saccharomyces cerevisiae microbial inoculum 1kg;
2, with after four kinds of microbial inoculum drying and crushing, mix, promptly get.
The become thoroughly decomposed preparation of agent of embodiment 2 microbial straws of the present invention
1, takes by weighing each microbial inoculum by following weight: bacillus subtilis microbial agent 2kg, black mold microbial inoculum 1kg, Trichodermareesei microbial inoculum 2kg, yeast saccharomyces cerevisiae microbial inoculum 0.5kg;
2, with after four kinds of microbial inoculum drying and crushing, mix, promptly get.
The become thoroughly decomposed preparation of agent of embodiment 3 microbial straws of the present invention
1, takes by weighing each microbial inoculum by following weight: bacillus subtilis microbial agent 4kg, black mold microbial inoculum 3kg, Trichodermareesei microbial inoculum 4kg, yeast saccharomyces cerevisiae microbial inoculum 1.5kg;
2, with after four kinds of microbial inoculum drying and crushing, mix, promptly get.
The intensification effect test of Test Example 1 single microbial inoculum
Straw powder after pulverizing packed into seal in the Cans, every bottle of 250g, 115 ℃ of sterilization 15min, inoculation after putting into refrigerator behind the low-temperature test naturally cooling and making its temperature drop to 8 ℃, the cold test direct inoculation, inoculum size 2 ‰ adds 5 ‰ urea simultaneously.The low-temperature test Cans are placed on to be put into 8 ℃ of refrigerators and cultivates in the thermos cup; The product test places 20 ℃ of incubators to cultivate; Low-temperature test was measured temperature in 12 hours at interval, and cellulase activity was measured in low temperature and cold test at interval in 12 hours in the single microbial inoculum single factor experiment.
The intensification effect of the following four kinds of bacterium of coldcondition is: black mold>subtilis>Trichodermareesei>S. cervisiae, wherein the zymic activity very a little less than, suitable with the intensification of blank.The intensification effect of black mold is best, possibly make system be rapidly heated because spore produces a large amount of biological heat when sprouting, and selected yeast is not high 8 ℃ of following activity.
The enzyme work of black mold, subtilis and three experimental group of Trichodermareesei has obvious lifting in the experiment of 20 ℃ of normal temperature; And Trichodermareesei>black mold>subtilis; And in 8 ℃ of experiments of low temperature; These three experimental group are measured enzymes and are lived and obvious lifting is also all arranged and be more or less the same, and the enzyme of yeast saccharomyces cerevisiae is lived all very lowly in twice test, and this also is as expected.And can find out that from the comparison that twice test enzyme lived the influence that temperature is lived to enzyme is that leading indicator comes the portfolio ratio of bacterial classification is optimized greater than the individual influence of three kinds of bacterium producing multi enzyme preparation black molds, subtilis and Trichodermareesei so select temperature.
2 four kinds of function yeast proportionings of Test Example gradient is confirmed the optimum proportion test
1, subtilis gradient test
In the 1-7 jar, respectively add black mold 0.1g, Trichodermareesei 0.1g, yeast saccharomyces cerevisiae 0.1g; Add subtilis 0g, 0.05g, 0.1g, 0.15g, 0.2g, 0.25g, 0.3g respectively at 7 jars again; Be respectively test group 1-7, put 8 ℃ of condition bottom fermentations in the refrigerator.
Through comparing the mensuration result of different experiments group, the optimal dose that mixes subtilis in the bacterium is 0.15g, and when promptly adding the 0.15g subtilis, the activity of the agent of becoming thoroughly decomposed performance is the strongest.Subtilis produces a large amount of gemma in the process of solid fermentation.Gemma is sprouted under appropriate condition, heats up thereby produce the great amount of heat system that makes.And subtilis can produce a large amount of cellulases, the Mierocrystalline cellulose in the decomposing straw powder.
2, black mold gradient test
In the 1-7 jar, respectively add Trichodermareesei 0.12g, yeast saccharomyces cerevisiae 0.1g and subtilis; Add black mold 0g, 0.05g, 0.1g, 0.15g, 0.2g, 0.25g, 0.3g respectively at 7 jars again; Be respectively test group 1-7, put 8 ℃ of condition bottom fermentations in the refrigerator.Wherein subtilis dosage is confirmed by subtilis dose gradient test-results.
Through comparing the mensuration result of different experiments group, the optimal dose that mixes black mold in the bacterium is 0.1g, and when promptly adding the 0.10g black mold, the activity of the agent of becoming thoroughly decomposed performance is the strongest.Contain a large amount of black spores in the black mold of solid fermentation production, spore is sprouted under suitable temperature and humidity.In the black mold microbial inoculum, contain a large amount of cellulases, can decomposition of cellulose as the carbon source material of spore germination and mycelial growth.
3, Trichodermareesei gradient test
In the 1-7 jar, respectively add yeast saccharomyces cerevisiae 0.1g and black mold and subtilis; Add Trichodermareesei 0g, 0.05g, 0.1g, 0.15g, 0.2g, 0.25g, 0.3g respectively at 7 jars again; Be respectively test group 1-7, put 8 ℃ of condition bottom fermentations in the refrigerator.Wherein subtilis dosage is confirmed by subtilis dose gradient test-results; Black mold dosage is confirmed by black mold dose gradient test-results.
Through comparing the mensuration result of different experiments group, the optimal dose that mixes Trichodermareesei in the bacterium is 0.15g, and when promptly adding the 0.15g Trichodermareesei, the activity of the agent of becoming thoroughly decomposed performance is the strongest.Testing used Trichodermareesei is the cellulose mycophenolate superior strain, contains a large amount of cellulases in the microbial inoculum of solid fermentation, and the Mierocrystalline cellulose in can the decomposing straw powder is as carbon source.
4, yeast saccharomyces cerevisiae gradient test
In the 1-7 jar, respectively add black mold, the mould and subtilis of wood, add yeast saccharomyces cerevisiae 0g, 0.05g, 0.1g, 0.15g, 0.2g, 0.25g, 0.3g respectively at 7 jars again, be respectively test group 1-7, put 8 ℃ of condition bottom fermentations in the refrigerator.Wherein subtilis dosage is confirmed by subtilis dose gradient test-results; Black mold dosage is confirmed by black mold dose gradient test-results; The mould dosage of wood is confirmed by Trichodermareesei dose gradient test-results.
Through comparing the mensuration result of different experiments group; Each addition group intensification difference on effect of yeast saccharomyces cerevisiae is little; Have than evident difference with control group behind 36h but add the yeast saccharomyces cerevisiae group, and the single bacterium elevated temperature test explanation in front, single yeast saccharomyces cerevisiae is not had a low temperature thermogenic action; Here the difference that embodies should be embodied by black mold, subtilis and Trichodermareesei growth differences; And each yeast interpolation group difference is not obvious, considers economic cause, so select minimum dose group 0.05g to be the righttest addition.
Through summing up and relatively four kinds of single bacterium gradients tests in front, obtain at last stalk become thoroughly decomposed in the optimum proportion of four kinds of test strains be subtilis: black mold: Trichodermareesei: yeast saccharomyces cerevisiae=3: 2: 3: 1.
The become thoroughly decomposed dosage optimization test of agent of Test Example 3 microbial straws of the present invention
Press subtilis: black mold: Trichodermareesei: yeast saccharomyces cerevisiae=3: 2: 3: 1 optimized proportion is respectively by 1 ‰, 1.5 ‰, 2 ‰; 2.5 ‰; 3 ‰ inoculum size inserts microbial inoculum, and low-temperature test is put 8 ℃ of condition bottom fermentations in the refrigerator, and cold test places 20 ℃ of incubators to ferment.Low-temperature test 18 days was measured temperature and cellulase activity at interval in 3 days, and cellulase activity was measured in cold test 12 days at interval in 2 days.
From normal temperature and low-temperature test result; In low temperature rise environment, 2 ‰ experimental group intensification abilities are the strongest, and the cellulase activity that 3 ‰ experimental group are produced is the highest; From absolute value; 2 ‰, the 2.5 ‰ and 3 ‰ experimental group intensification abilities and the ability difference of becoming thoroughly decomposed also are so in cold test not quite and all far above 1 ‰ and 1.5 ‰ two experimental group, are optimum quantity so select 2 ‰ consumptions.
The become thoroughly decomposed agent rice straw pilot scale compliance test result test of Test Example 4 microbial straws of the present invention
With the woven bag of 120*60cm, every packed 5kg rice straw of going into, the total consumption by 2 ‰ adds embodiment 1 prepared composite microbial inoculum and 25g urea (test group); Control group only adds urea, adds water saturates, and it is dark to imbed underground 15cm; Tiling plastics film in woven bag below prevents that the moisture seepage runs off, and becomes thoroughly decomposed 21 days; Measured temperature in per three days, cellulase activity and humic acids content, rate of emergence is measured in the back of finishing dealing with.
Temperature variation result: preceding 12 days temperature of control group slowly rise; Be raised to real 19.2 ℃ of top temperature by the 12nd day, back temperature slowly descends, and high temperature does not reach 20 ℃; And preceding 9 days of test group are the temperature rise period; The temperature of becoming thoroughly decomposed rises to 22.4 ℃ from 11.4 ℃, and the 9th day to 15 days temperature continue to keep the quick composting that higher temperature helps rice straw more than 20 ℃.
The cellulase activity result of variations: in 21 days of test, the control group cellulase activity continued slowly to rise always, finally rose to 118.6Ug-1 at the 21st day; And test group was the fastest cellulase activity rising in preceding 9 days, promptly reached 266.4Ug-1 at the 9th day, after the 9th day, slowly rose; And rose to 342.66Ug-1 at the 21st day; No matter be the enzyme absolute value that the speed that rises or enzyme live of living, experimental group all obviously is superior to control group, explains that the product of the present invention effect of becoming thoroughly decomposed is remarkable.
Humic acids content result: control group and test group humic acids content in digest process all is to continue to increase; But just from lift velocity and content; Test group all obviously is superior to control group, explains that to use after the agent of originally becoming thoroughly decomposed the effect of becoming thoroughly decomposed to rice straw obvious.
Rate of emergence (GI) test-results: all than higher and difference is little, the degree of becoming thoroughly decomposed that rice straw is described is to not significantly influence of rate of emergence for three test group GI values.
The agent wheat stalk pilot scale compliance test result test brief summary of becoming thoroughly decomposed of Test Example 5 microbial straws of the present invention
With the woven bag of 120*60cm, every packed 5kg wheat stalk of going into, the total consumption by 2 ‰ adds the embodiment of the invention 2 prepared composite microbial inoculums and 25g urea (experimental group); Control group only adds urea, adds water saturates, and it is dark to imbed underground 15cm; Tiling plastics film in woven bag below prevents that the moisture seepage runs off, and becomes thoroughly decomposed 21 days; Measured temperature in per three days, cellulase activity and humic acids content, rate of emergence is measured in the back of finishing dealing with.
Temperature variation result: preceding 12 days temperature of control group slowly rise; Be raised to real 18.8 ℃ of top temperature by the 12nd day, back temperature slowly descends, and high temperature does not reach 20 ℃; And preceding 9 days of experimental group are the stage of being rapidly heated; The temperature of becoming thoroughly decomposed rises to 21.8 ℃ from 11.4 ℃, and the 9th day to 15 days temperature continue to keep the quick composting that higher temperature helps wheat stalk more than 20 ℃.
The cellulase activity result of variations: in 21 days of test, the control group cellulase activity continued slowly to rise always, finally rose to 112.4Ug-1 at the 21st day; And experimental group was the fastest cellulase activity rising in preceding 9 days, promptly reached 287.2Ug-1 at the 12nd day, after the 12nd day, slowly rose; And rose to 336.8Ug-1 at the 21st day; No matter be the enzyme absolute value that the speed that rises or enzyme live of living, experimental group all obviously is superior to control group, explains that the agent product that originally the becomes thoroughly decomposed effect of becoming thoroughly decomposed is remarkable.
Humic acids content result: control group and experimental group humic acids content in digest process all is to continue to increase; But just from lift velocity and content; Experimental group all obviously is superior to control group, explains that to use after the agent of originally becoming thoroughly decomposed the effect of becoming thoroughly decomposed to wheat stalk obvious.
Rate of emergence (GI) test-results: all than higher and difference is little, the degree of becoming thoroughly decomposed that wheat stalk is described is to not significantly influence of rate of emergence for three experimental group GI values.
The agent plot experiment brief summary as a result of becoming thoroughly decomposed of Test Example 5 microbial straws of the present invention
1 Huang Zhou district king Yu office test-results
1.1, different treatment is to the stalk velocity effect of becoming thoroughly decomposed
The microbial straw of the present invention agent of becoming thoroughly decomposed is handled: 3 days variable color → 7 day putresce → and to rot in 14 days 50% or more → all rotted in 22 days, the rotten speed of straw is fast;
The equivalent fine earth is handled: putresced in 14 days → only rotted 30% in 22 days, the straw speed of rotting is slow;
Straw is handled: rotting rate had only about 20% in 22 days, and the rotten speed of straw is the slowest.
Can find out from the straw speed of becoming thoroughly decomposed of different treatment; Microbial straw of the present invention becomes thoroughly decomposed agent owing to contain mushrooms such as a large amount of Mierocrystalline celluloses, semicellulose and xylogen, has promoted the straw quick composting, and then has improved soil organic matter content; Improve the soil nutrient situation, increase soil fertility.
1.2, different treatment is to the become thoroughly decomposed influence of degree of straw
The microbial straw of the present invention agent of becoming thoroughly decomposed is handled: straw all rots, and color becomes brown and chocolate, and soil color is also deepened to some extent, and the solid degree of soil lightens;
The equivalent fine earth is handled: though color is deepened to some extent, stalk is harder, and the overwhelming majority is not rotted, and the solid degree of soil does not have to change basically;
Explain that microbial straw of the present invention becomes thoroughly decomposed in the agent mikrobe under optimum conditions; Give full play to its Decomposition, straw is fully become thoroughly decomposed, soil organic matter content is increased; The paddy soil physicochemical character improves, thereby haves laid a good foundation for improving crop yield.
1.3, experiment conclusion
The microbial straw effect of agent on straw that become thoroughly decomposed according to the present invention proves that the microbial straw of the present invention agent (microbial preparation) of becoming thoroughly decomposed has the effect of becoming thoroughly decomposed preferably to straw; The one, accelerate the straw speed of becoming thoroughly decomposed, under suitable condition, about 20 days just straw become thoroughly decomposed basically; The 2nd, heighten the straw degree of becoming thoroughly decomposed, after being rotted, straw darkens, be brown or chocolate, and the soil texture lightens, physicochemical character improves, and this promotes that to increasing soil fertility agricultural sustainable development is significant.
2, Wuxue City Mei Chuan town test-results
Test-results shows, putresces with the microbial straw of the present invention three days rice straws of agent treatment group that become thoroughly decomposed, and shows as the field temperature and is higher than contrast B (add water and urea does not add the agent of becoming thoroughly decomposed), and water is slightly acidic; Most of vascular bundle of seven days aftertreatment group rice straws and the mesophyll epidermis that comes off rot to be netted, and control group B putresces; Handle after ten days, the agent group of becoming thoroughly decomposed is rotten fully, and it is netted that bar is, and leaf is broken wadding to be adorned, and handles after 15 days stalk and rots into pasty state (rotting fully, it is ripe to macerate) in whole rotten process, seedling growth is normal.
Rice straw uses microbial straw of the present invention to become thoroughly decomposed after agent adds urea and handle, and compares with independent use urea, and it putresces early 7 days time, and safety rots to macerate 10 days ripe morning; And rotten degree is superior to the latter, and the speed of the rotting degree of still rotting all has notable difference, and does not influence paddy growth.Therefore the microbial straw of the present invention agent of becoming thoroughly decomposed can be used on paddy rice.
The Test Example 5 microbial straws of the present invention agent field experiment of becoming thoroughly decomposed
1, microbial straw of the present invention becomes thoroughly decomposed agent to wheat-corn crop rotation field field experiment of becoming thoroughly decomposed also on the spot
The variation of A, soil porosity
Wheat straw on the spot also the Tian Qianzhi seeding stage handle 1 and 2 soil porosities and drop to 44.2% and 56.4% by 55.4% respectively and drop to 46.6%, descend 11.2 and 9.8 percentage points.And contrasting 1 and 2 only 4.9 and 6.3 percentage points, fall is obvious.Explain that adding microbial straw of the present invention becomes thoroughly decomposed and accelerated the rotten degree of wheat straw after the agent, improved soil porosity, favourable soil moisturizing, protect fertilizer and crop root growth.
The variation of B, the soil organism
Also handle 1 and 2 soil organism respectively by 14.8 rise to 32.3 and 13.6 rise to 31.8, every kg soil organic matter content respectively rise 17.5g and 18.2g on the spot by the Tian Qianzhi seeding stage for wheat straw.And contrasting 1 and 2 every kg soil organic matter contents rise respectively 4.9g and 7.7g, ascensional range is obvious.Help strengthening soil fertility.
C, the material color of becoming thoroughly decomposed, material structure change
Also the field is to the sowing 15 day time of corn on the spot from straw, and it is dark that the color treatments of wheat straw contrasts color, draws out material and observes, and is all softer; But arrived the emergence of corn phase when observing wheat straw once more and becoming thoroughly decomposed proterties, the color of treatment group contrast is darker, becomes and ravels with the microbial straw of the present invention wheat straw of agent (embodiment 1-3 is prepared) after handling of becoming thoroughly decomposed, and is soft state and contrast.Show the difference of obviously becoming thoroughly decomposed.From corn growth time in seedling stage, infer that processing contrasts rotten degree and shifts to an earlier date 20-30 days.
D, C/N are than changing
The average C/N ratio of corn seeding phase treatment group is 23.9: 1, and standard C/N ratio is 20: 1 a reference value near becoming thoroughly decomposed; And the average C/N ratio between contrast is 33.95: 1, and surpassing the standard C/N ratio that becomes thoroughly decomposed is 20: 1 reference value.Arrived the emergence of corn phase, the average C/N ratio of treatment group is 20.55: 1, and reaching the standard C of becoming thoroughly decomposed/N ratio basically is 20: 1 reference value; And the average C/N between contrast is to be 27.1: 1, and the average C/N that surpasses corn seeding phase treatment group was than 23.9: 1.Show that adding microbial straw of the present invention becomes thoroughly decomposed and accelerated the rotten degree of wheat straw, observations supposition basically identical among become thoroughly decomposed in advance time and the C after the agent (embodiment 1-3 is prepared).
E, wheat straw are also sowed the bud ratio of corn and the mutation analysis of output behind the field on the spot
On average bud ratio 84.9% is high 11.3 percentage points between contrast for the average bud ratio high 98.2% of treatment group.The corn mean yield of treatment group is 3082 kilograms, flat 308 kilograms of mu, and only 2683 kilograms of the mean yields of contrast, flat 268 kilograms of mu, 40 kilograms of the flat raising the output of mu, rate of increase 7.4%.
F, conclusion
Wheat straw is the maize planting and become thoroughly decomposed in the field on the spot; Become thoroughly decomposed color, material structure and water-soluble carbon nitrogen than analyzing from little wheat stalk; Added the rotten degree of having accelerated wheat straw after (embodiment 1-3 is prepared); Make the wheat straw time of becoming thoroughly decomposed shift to an earlier date 20-30 days, the corn bud ratio improves 11.3 percentage points, and output improves 7.4%.
Test Example 6 microbial straws of the present invention become thoroughly decomposed agent to corn-wheat crop rotation field field experiment of becoming thoroughly decomposed also on the spot
The variation of A, soil porosity
Wheat straw on the spot also the Tian Qianzhi seeding stage handle 1 and 2 soil porosities and drop to 46.2% and 57.5% by 58.6% respectively and drop to 47.5%, descend 12.4 and 10 percentage points.And contrasting 1 and 2 only 8.88 and 7.2 percentage points, fall is obvious.Explain that adding microbial straw of the present invention becomes thoroughly decomposed and accelerated the rotten degree of corn stalk after the agent (embodiment 1-3 is prepared), improved soil porosity, favourable soil moisturizing, protect fertilizer and crop root growth.
The variation of B, the soil organism
Also handle 1 and 2 soil organism respectively by 16.5 rise to 31.3 and 14.6 rise to 30.8, every kg soil organic matter content respectively rise 14.8g and 16.2g on the spot by the Tian Qianzhi seeding stage for corn tangerine bar.And contrasting 1 and 2 every kg soil organic matter contents rise respectively 6.4g and 8.0g, ascensional range is obvious.Help strengthening soil fertility.
C, the material color of becoming thoroughly decomposed, material structure change
Also the field is to the sowing 30 day time of wheat on the spot from straw, and it is dark that the color treatments of corn stalk contrasts color, draws out material and observes, and treatment group is soft, frangible, and control group is soft slightly, the difficulty is broken; But arrived the wheat seeding stage when observing corn stalk once more and becoming thoroughly decomposed proterties, the color of treatment group contrast is darker, becomes soft broken with the microbial straw of the present invention corn stalk of agent (embodiment 1-3 is prepared) after handling that become thoroughly decomposed, and is soft state and contrast.Show the difference of obviously becoming thoroughly decomposed.From wheat growth time in seedling stage, infer that processing contrasts rotten degree and shifts to an earlier date 20-30 days.
D, C/N are than changing
The average C/N ratio of sowing stage of wheat treatment group is 28.8: 1, surpasses to become thoroughly decomposed standard C/N than 8.8 points of 20: 1 of reference value; And the average C/N ratio between contrast is 38.4: 1, surpasses 18.4 points of standard C/N than 20: 1 reference values that become thoroughly decomposed.Arrived the wheat seeding stage, the average C/N ratio of treatment group is 23.0: 1, near becoming thoroughly decomposed the reference value of standard C/N than 20: 1; And the average C/N between contrast is to be 28.9: 1, surpasses 5.8 points of average C/N than 23.0: 1 of sowing stage of wheat treatment group.Show that adding microbial straw of the present invention becomes thoroughly decomposed and accelerated the rotten degree of corn stalk, time and the C observations of becoming thoroughly decomposed in advance supposition basically identical after the agent (embodiment 1-3 is prepared).
E, corn stalk are also sowed the bud ratio of wheat and the mutation analysis of output behind the field on the spot
On average bud ratio 86.5% is high 9.6 percentage points between contrast for the average bud ratio 96.1% of treatment group.The wheat mean yield of treatment group is 4130 kilograms, flat 413 kilograms of mu, and only 3936 kilograms of the mean yields of contrast, flat 393.6 kilograms of mu, 19.4 kilograms of the flat raising the output of mu, rate of increase 4.9%.
F, conclusion
Corn stalk is the plantation wheat and become thoroughly decomposed in the field on the spot; Become thoroughly decomposed color, material structure and water-soluble carbon nitrogen than analyzing from corn tangerine bar; Add the microbial straw of the present invention agent (embodiment 1-3 is prepared) of becoming thoroughly decomposed and accelerated the rotten degree of corn stalk; Make the corn stalk time of becoming thoroughly decomposed shift to an earlier date 20-30 days, the wheat bud ratio improves 9.6 percentage points, and output improves 4.9%.
Test Example 7 microbial straws of the present invention become thoroughly decomposed agent (embodiment 1-3 is prepared) to wheat-paddy rice crop rotation field field experiment of becoming thoroughly decomposed also on the spot
The variation of A, soil porosity
Wheat straw on the spot also the Tian Qianzhi seeding stage handle 1 and 2 soil porosities and drop to 42.2% and 56.8% by 54.4% respectively and drop to 46.6%, descend 12.2 and 13.2 percentage points.And contrasting 1 and 2 only 7.2 and 9.2 percentage points, fall is obvious.Explain that adding microbial straw of the present invention becomes thoroughly decomposed and accelerated the rotten degree of wheat straw after the agent, has improved soil porosity, favourable rice root growth.
The variation of B, the soil organism
Also the Tian Qianzhi rice transplanting pre-treatment 1 and 2 soil organism rise to 33.8 and 13.4 by 13.6 respectively and rise to 34.6, every kg soil organic matter content rise respectively 20.2g and 21.2g wheat straw on the spot.And contrasting 1 and 2 every kg soil organic matter contents rise respectively 7.0g and 8.7g, ascensional range is obvious.Help strengthening soil fertility.
C, the material color of becoming thoroughly decomposed, material structure change
Also the field is to the preceding 30 day time of rice transplanting on the spot from straw, and wheat straw becomes and ravels with " Shi Ruifu " biological straw wheat straw of agent after handling of becoming thoroughly decomposed, and contrast is soft state.Show the difference of obviously becoming thoroughly decomposed.The physicochemical character analysis of wheat straw before the rice transplanting is inferred to handle to contrast rotten degree and shift to an earlier date 15-20 days.
D, C/N are than changing
The average C/N ratio of rice transplanting pre-treatment group is 19.4: 1, is lower than the reference value of standard C/N than 20: 1 of becoming thoroughly decomposed; And the average C/N ratio between contrast is 28: 1, is higher than the standard C of becoming thoroughly decomposed/N ratio and is 8 points of reference value of 20: 1.Show that adding microbial straw of the present invention becomes thoroughly decomposed and accelerated the rotten degree of wheat straw, time and the C observations of becoming thoroughly decomposed in advance supposition basically identical after the agent.
E, wheat straw are gone back the mutation analysis of the output behind the field on the spot
The paddy rice mean yield of treatment group is 1365 kilograms, flat 650 kilograms of mu, and only 1218 kilograms of the mean yields of contrast, flat 580 kilograms of mu, 70 kilograms of the flat raising the output of mu, rate of increase 7.96%.
F, conclusion
Wheat straw is the rice cultivation and become thoroughly decomposed in the field on the spot; Become thoroughly decomposed color, material structure and water-soluble carbon nitrogen than analyzing from little wheat stalk; Added microbial straw of the present invention and become thoroughly decomposed and accelerated the rotten degree of wheat straw after the agent, made the wheat straw time of becoming thoroughly decomposed shift to an earlier date 15-20 days, rice yield improves 7.96%.
Test Example 8 microbial straws of the present invention become thoroughly decomposed agent (embodiment 1-3 is prepared) to paddy rice-wheat crop rotation field field experiment of becoming thoroughly decomposed also on the spot
The variation of A, soil porosity
The paddy rice bar drops to 40.5% and Tian Qianzhi wheat seeding stage processing 1 and 2 soil porosities drop to 41.2% and 55.5% by 54.3% respectively on the spot, descends 13.1 and 15 percentage points.And contrasting 1 and 2 only 7.0 and 8.3 percentage points, fall is obvious.Explain that adding microbial straw of the present invention becomes thoroughly decomposed and accelerated the rotten degree of paddy rice bar after the agent, improved soil porosity, favourable soil moisturizing, protect fertilizer and crop root growth.
The variation of B, the soil organism
Also handle 1 and 2 soil organism respectively by 15.4 rise to 35.6 and 15.6 rise to 38.8, every kg soil organic matter content respectively rise 20.2g and 23.2g on the spot by the Tian Qianzhi wheat seeding stage for paddy rice tangerine bar.And contrasting 1 and 2 every kg soil organic matter contents rise respectively 11g and 13.5g, ascensional range is obvious.Help strengthening soil fertility.
C, the material color of becoming thoroughly decomposed, material structure change
Find out that from last table also the field is to the sowing 30 day time of wheat on the spot from paddy stalk, it is dark that the color treatments of paddy rice bar contrasts color, draws out material and observe, and treatment group is soft, frangible, though and control group is soft, difficult broken; But arrived the wheat seeding stage when observing the paddy rice bar once more and becoming thoroughly decomposed proterties, the color of treatment group contrast is darker, becomes soft broken with the microbial straw of the present invention paddy rice bar of agent after handling that become thoroughly decomposed, and is soft state and contrast.Show the difference of obviously becoming thoroughly decomposed.From wheat growth time in seedling stage, infer that processing contrasts rotten degree and shifts to an earlier date 20-30 days.
D, C/N are than changing
Find out that from last table the average C/N ratio of sowing stage of wheat treatment group is 21.7: 1, near the standard C/N that becomes thoroughly decomposed than reference value 20: 1; And the average C/N ratio between contrast is 34.9: 1, surpasses 14.9 points of standard C/N than 20: 1 reference values that become thoroughly decomposed.Arrived the wheat seeding stage, the average C/N ratio of treatment group is 19.0: 1, is lower than the reference value of standard C/N than 20: 1 of becoming thoroughly decomposed; And the average C/N between contrast is to be 27.5: 1, surpasses 7.5 points of average C/N than 20.0: 1 of sowing stage of wheat treatment group.Show that adding microbial straw of the present invention becomes thoroughly decomposed and accelerated the rotten degree of paddy rice bar, time and the C observations of becoming thoroughly decomposed in advance supposition basically identical after the agent.
E, paddy rice bar are also sowed the bud ratio of wheat and the mutation analysis of output behind the field on the spot
Find out that from last table on average bud ratio 87.3% is high 6.3 percentage points between contrast for the average bud ratio 93.6% of treatment group.The wheat mean yield of treatment group is 844.5 kilograms, flat 402 kilograms of mu, and the mean yield of contrast is 801 kilograms, flat 381 kilograms of mu, 21 kilograms of the flat raising the output of mu, rate of increase 5.5%.
F, conclusion
Paddy stalk is the plantation wheat and become thoroughly decomposed in the field on the spot; Become thoroughly decomposed color, material structure and water-soluble carbon nitrogen than analyzing from paddy rice tangerine bar; Having added microbial straw of the present invention becomes thoroughly decomposed and has accelerated the rotten degree of paddy rice bar after the agent; Make the paddy stalk time of becoming thoroughly decomposed shift to an earlier date 20-30 days, the wheat bud ratio improves 6.3 percentage points, and output improves 5.5%.
Test Example 9 mikrobe straws of the present invention become thoroughly decomposed agent (embodiment 1-3 is prepared) to paddy rice-rape crop rotation field field experiment of becoming thoroughly decomposed also on the spot
The variation of A, soil porosity
The paddy rice bar drops to 40.2% and Tian Qianzhi rape seeding stage processing 1 and 2 soil porosities drop to 41.4% and 55.2% by 54.5% respectively on the spot, descends 13.1 and 15 percentage points.And contrasting 1 and 2 only 7.0 and 8.3 percentage points, fall is obvious.Explain that adding mikrobe straw of the present invention becomes thoroughly decomposed and accelerated the rotten degree of paddy rice bar after the agent, improved soil porosity, favourable soil moisturizing, protect fertilizer and crop root growth.
The variation of B, the soil organism
Also handle 1 and 2 soil organism respectively by 15.6 rise to 35.1 and 15.2 rise to 38.4, every kg soil organic matter content respectively rise 19.5g and 23.1g on the spot by the Tian Qianzhi rape seeding stage for paddy rice tangerine bar.And contrasting 1 and 2 every kg soil organic matter contents rise respectively 10.8g and 13g, ascensional range is obvious.Help strengthening soil fertility.
C, the material color of becoming thoroughly decomposed, material structure change
Find out that from test-results also the field is to the sowing 30 day time of rape on the spot from paddy stalk, it is dark that the color treatments of paddy rice bar contrasts color, draws out material and observe, and treatment group is soft, frangible, though and control group is soft, difficult broken; But arrived the rape seeding stage when observing the paddy rice bar once more and becoming thoroughly decomposed proterties, the color of treatment group contrast is darker, becomes soft broken with the mikrobe straw of the present invention paddy rice bar of agent after handling that become thoroughly decomposed, and is soft state and contrast.Show the difference of obviously becoming thoroughly decomposed.From the rape seedling growth time, infer that processing contrasts rotten degree and shifts to an earlier date 20-30 days.
D, C/N are than changing
Find out that from test the average C/N ratio of rape seed phase treatment group is 22.3: 1, near the standard C/N that becomes thoroughly decomposed than reference value 20: 1; And the average C/N ratio between contrast is 30.8: 1, surpasses 14.9 points of standard C/N than 20: 1 reference values that become thoroughly decomposed.Arrived the rape seeding stage, the average C/N ratio of treatment group is 18.8: 1, is lower than the reference value of standard C/N than 20: 1 of becoming thoroughly decomposed; And the average C/N between contrast is 25.1: 1, surpasses 5.1 points of average C/N than 20.0: 1 of rape seed phase treatment group.Show that adding mikrobe straw of the present invention becomes thoroughly decomposed and accelerated the rotten degree of paddy rice bar, time and the 3-2-1 observations of becoming thoroughly decomposed in advance supposition basically identical after the agent.
E, paddy rice bar are also sowed the bud ratio of rape and the mutation analysis of output behind the field on the spot
On average bud ratio 87.3% is high 6 percentage points between contrast for the average bud ratio 93.3% of treatment group.The rape mean yield of treatment group is 1444.5 kilograms, flat 144.4 kilograms of mu, and the mean yield of contrast is 1326 kilograms, flat 132.6 kilograms of mu, 11.8 kilograms of the flat raising the output of mu, rate of increase 8.9%.
F, conclusion
Paddy stalk is the plantation rape and become thoroughly decomposed in the field on the spot; Become thoroughly decomposed color, material structure and water-soluble carbon nitrogen than analyzing from paddy rice tangerine bar; Having added mikrobe straw of the present invention becomes thoroughly decomposed and has accelerated the rotten degree of paddy rice bar after the agent; Make the paddy stalk time of becoming thoroughly decomposed shift to an earlier date 20-30 days, the rape bud ratio improves 6.3 percentage points, and output improves 8.9%.

Claims (7)

1. microbial straw agent of becoming thoroughly decomposed comprises following composition: subtilis (Bacillus subtilis) microbial inoculum, black mold (Aspergillus niger) microbial inoculum, Trichodermareesei (Trichoderma reesei) microbial inoculum, S. cervisiae (Saccharomyces cerevisiae) microbial inoculum.
2. according to the agent of becoming thoroughly decomposed of the described microbial straw of claim 1, it is characterized in that the weight part of each composition is: bacillus subtilis microbial agent 2-4 part, black mold microbial inoculum 1-3 part, Trichodermareesei microbial inoculum 2-4 part, yeast saccharomyces cerevisiae bacteria agent 0.5-1.5 part.
3. according to the agent of becoming thoroughly decomposed of the described microbial straw of claim 2, it is characterized in that the weight part of each composition is: 3 parts of bacillus subtilis microbial agents, 2 parts of black mold microbial inoculums, 3 parts of Trichodermareesei microbial inoculums, 1 part of yeast saccharomyces cerevisiae bacteria agent.
4. one kind prepares the become thoroughly decomposed method of agent of any one said microbial straw of claim 1-3, may further comprise the steps:
(1) prepares subtilis (Bacillus subtilis) microbial inoculum, black mold (Aspergillus niger) microbial inoculum, Trichodermareesei (Trichoderma reesei) microbial inoculum, S. cervisiae (Saccharomyces cerevisiae) microbial inoculum respectively;
(2) with after four kinds of prepared microbial inoculum drying and crushing, mix, promptly get.
5. any one said microbial straw of the claim 1-3 purposes of agent in the field stalk also of becoming thoroughly decomposed of becoming thoroughly decomposed.
6. according to the described purposes of claim 5; It is characterized in that; Comprise: convert water 30-50 kilogram by 5 kilograms in every mu of 2 kilograms+urea and evenly spray on stalk surface with the agent of becoming thoroughly decomposed of said microbial straw; Together plough under the agent of becoming thoroughly decomposed of agricultural crop straw and microbial straw of the present invention underground then, the degree of depth keeps soil humidity at 50-60% at 10-15 centimetre.
7. according to the described purposes of claim 5; It is characterized in that, comprising: add the agent of becoming thoroughly decomposed of the said microbial straw of 0.5-1.0 kilogram by tangerine bar per ton, water waters the tangerine bar then; With the stalk dry weight basis; The diammonium phosphate of adding 2-5% or ight soil water and stalk mix when watering, and the stalk water cut is controlled at 60-70wt%, play the heap back and cover with the plastics film abdomen.
CN2011102909848A 2011-09-29 2011-09-29 Microbial straw decomposition additive as well as preparation method and application thereof Pending CN102382768A (en)

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CN108083859A (en) * 2018-01-20 2018-05-29 商丘职业技术学院 A kind of wheat stalk quick rotting agent and preparation method thereof
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