CN104609995A - Plant growth promoting bio-organic fertilizer for saline-alkali land - Google Patents
Plant growth promoting bio-organic fertilizer for saline-alkali land Download PDFInfo
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- CN104609995A CN104609995A CN201510030096.0A CN201510030096A CN104609995A CN 104609995 A CN104609995 A CN 104609995A CN 201510030096 A CN201510030096 A CN 201510030096A CN 104609995 A CN104609995 A CN 104609995A
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- trichoderma asperellum
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses plant growth promoting bio-organic fertilizer for saline-alkali land, and belongs to the technology of soil improvement. The bio-organic fertilizer produced by fermenting agricultural organic solid wastes through microbial strains can increase the survival rate and promote growth of salt-tolerant plants planted in mud-flat soil and increases a soil fertility improvement speed and speeds up an improvement progress. Compost is subjected to secondary fermentation by trichoderma asperellum 12 and bacillus subtilis D9, wherein the content of trichoderma asperellum 12 in a product is larger than or equal to 0.2*10<8> cfu (colony forming unit) g<-1>, the content of bacillus subtilis D9 in the product is larger than or equal to 0.5*10<8> cuf g<-1>, a pH value is 5.5, the mass ratio of the water content is smaller than or equal to 30% and the mass ratio of organic matter content is larger than or equal to 45%. An experiment indicates that compared with treatment without application of the bio-organic fertilizer, the biomass of salt-tolerant plants including suaeda galuce, cyperus malaccensis and kochia scoparia planted in soil is increased by 49.1%, 56.3% and 52.2 respectively after the bio-organic fertilizer is applied to the mud-flat saline-alkali soil.
Description
Technical field
The present invention relates to a kind of saltings growth-promoting biological organic fertilizer, belong to microbial technology field.
Background technology
There is the continent water front of 1.8 ten thousand kilometers in China, and because Sediment Siltation forms a large amount of beach soil, the high-quality exploitation of mud-flat soil, can provide a large amount of agricultural lands, for society brings huge economic benefit and ecological environment benefit.
Improvement main method in beach saline-alkali ground is by the plantation salt-tolerant plant that digs trenches to drain the water away, and digs trenches to drain the water away and is conducive to Infiltration, accelerate desalination.Because most beach is used for the fresh water of wash-out soil salt, main dependence precipitation, and precipitation mainly concentrates on the summer of the 6-8 month, the precipitation that short period of time is a large amount of, if the vegetation that saltings mud-flat soil covers is less, form with rainwash flows away by rainfall major part, is difficult to reach infiltrate the object of the desalinization of soil by flooding or leaching.And except 6-8 month, other in month the transpiration rate of soil be the several times of quantity of precipitation, can not effectively reduce earth's surface transpiration, be easy to cause the soil accumulation of salt in the surface soil.Therefore, plantation salt tolerant crop, the improved speed for mud-flat soil has conclusive impact.
Beach saline-alkali ground due to structure poor, soil beneficial microorganism content is less, and the salt-tolerant plant speed of growth of plantation is slow, and biomass is lower, the serious improvement that have impact on soil.Growth-promoting microorganism (Plant Growth Promoting Rhizobacteria, PGPR or Plant Growth Promoting Fungi, PGPF) growth promoting plant has been widely used in, because growth-promoting microorganism can secrete plant hormone, fixing nitrogen or activation phosphorus element etc.But beach saline-alkali ground, saltiness is high, and soil transpiration rate is fast, requires higher to the resistance of functional microorganism.
Document 1 is the granted patent of publication number CN201310307561.1, a kind of biocontrol strains, biological organic fertilizer and preparation method thereof of prevention and control rice sheath blight disease are disclosed, invention utilize to rice sheath blight disease have remarkable biological control effect Trichoderma asperellum strain 12 (
trichoderma asperellum12), this biocontrol strains and rice husk, stalk, livestock excrement composting is utilized to make bio-organic fertilizer, containing 0.5 × 10 in fertilizer
8cfu g
-1above this bacterial strain statospore, water content for being less than 15%, organic content is greater than 35%.
Obviously, document 1 provides a kind of microbial organic fertilizer of biological and ecological methods to prevent plant disease, pests, and erosion rice sheath blight disease, but not promotes the microbial fertilizer of beach saline-alkali soil plantation salt tolerant crop growth.
Document 2 is the patent application of publication number CN201410261688.9, discloses a kind of saltings growth-promoting biological organic fertilizer and preparation method thereof, utilizes bacillus subtilis strain and Li's Trichoderma strains, produces reed wormwood artemisia Straw decomposing microbial inoculum.This microbial inoculum can accelerate the decomposition rate of reed wormwood artemisia crop straw stubble in reed wormwood artemisia continuous cropping soil, suppresses the breeding hyperplasia of pathogenic micro-organism on crop straw stubble simultaneously, reduces the harm of reed wormwood artemisia continuous cropping obstacle.Functional microorganism quantity>=2 × 10 in microbial inoculum
8cfu g
-1, water content is less than 15%, and organic content is greater than 35%.
Obviously, document 2 provides a kind of saltings growth-promoting biological organic fertilizer and preparation method thereof, but not promotes the microbial fertilizer of beach saline-alkali soil plantation salt tolerant crop growth.
Document 3 is publication number CN201410262147.8 patent application, disclose and a kind of there is microbial organic fertilizer preventing and kill off reed wormwood artemisia continuous cropping obstacle effect and preparation method thereof, utilize bacillus subtilis bacteria solid fermentation agricultural organic solid castoff, produce the microbial organic fertilizer preventing and kill off reed wormwood artemisia continuous cropping obstacle.Functional microorganism quantity>=2 × 10 in this microbial organic fertilizer
8cfu g
-1, water content is less than 30%, and organic content is greater than 30%.
Obviously, document 3 provides a kind of and has microbial organic fertilizer preventing and kill off reed wormwood artemisia continuous cropping obstacle effect and preparation method thereof, but not promotes the microbial fertilizer of beach saline-alkali soil plantation salt tolerant crop growth.
Therefore, developing a kind of saltings growth-promoting biological organic fertilizer, improve serike surviving rate and the speed of growth, is the effective way accelerating beach saline-alkali soil improvement.Along with social development; to the attention all the more of environment protection; adopt nuisanceless, efficient, sustainable bio-technology improvement soil to suit social demand all the more, therefore, to beach saline-alkali the salt-tolerant plant of the planting biological organic fertilizer with growth-promoting functions there are very large market outlook.
Up to now, the bibliographical information finding to relate to present subject matter is not had.
Summary of the invention
Object: in order to overcome the deficiencies in the prior art, the invention provides a kind of saltings growth-promoting biological organic fertilizer, biological organic fertilizer utilize be separated to from beach saline-alkali soil and salinification plastic shed soil respectively, can the trichoderma asperellum 12 of Promoting plant growth and subtilis D9, Secondary Fermentation agricultural solid residue is made, in product, functional microorganism bacterial strain can adapt to salt affected soil poor environment, plays the effect of growth promoting effects.
Technical scheme: for solving the problems of the technologies described above, the technical solution used in the present invention is:
A kind of saltings growth-promoting biological organic fertilizer, is characterized in that: described saltings growth-promoting biological organic fertilizer is for making by trichoderma asperellum 12 and subtilis D9 fermentation agricultural solid residue.
Described trichoderma asperellum 12(
trichoderma asperellum12), on May 16th, 2013 is preserved in China typical culture collection center, and preservation address is Luo Jia Shan, wuchang, wuhan, and bacterial strain preserving number is CCTCC NO:M2013213.Described subtilis D9(
bacillus subtilisd9
)be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on May 16th, 2014, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, and bacterial strain preserving number is CGMCC NO.9170.The Main Biological of subtilis D9 is: bacterium colony is for white, edge is irregular, surface drying is opaque, Gram-positive, tool mobility, by electron microscopic observation, shaft-like, raw in gemma, the blunt circle in two ends.
In the growth-promoting biological organic fertilizer of described saltings, trichoderma asperellum 12 quantity>=0.2 × 10
8cfu g
-1, subtilis D9>=0.5 × 10
8cfu g
-1, pH value 5.5, water content mass ratio≤30%, quality of organic matter is than content>=45%.
The preparation method of above-mentioned saltings growth-promoting biological organic fertilizer, is characterized in that, comprise the following steps:
1) trichoderma asperellum 12 is inoculated into PDA nutrient solution, carry out liquid fermenting production, the condition of its fermentative production is: initial pH scope is 6.5-7.2, culture temperature 25-30 DEG C, dissolved oxygen air flow scope is 30-100%, 170rpm, fermentation later stage mycelium pellet is all broken into mycelia fragment and obtains trichoderma asperellum 12 fermented liquid, colony-forming unit>=1 × 10 of trichoderma asperellum 12 fermented liquid bacterial strain
8cfu ml
-1;
2) by vinegar grain: wheat bran 5:1 mixing in mass ratio, 120 DEG C of sterilizing 30min make solid fermentation matrix; Be inoculated in solid fermentation matrix by trichoderma asperellum 12 fermented liquid, the inoculum size of trichoderma asperellum 12 fermented liquid is the volume ratio of solid fermentation matrix 5%, ferments 6 days, and oven drying at low temperature obtains trichoderma asperellum 12 solid fermentation bacterial classification;
3) subtilis D9 is inoculated into beef soup peptone liquid fermentation medium, produce subtilis D9 fermented liquid, the condition of its fermentative production is: initial pH scope is 6.5-7.2, culture temperature 35-37 DEG C, dissolved oxygen air flow scope is 30-100%, 120-180rpm, Number of spores>=2 × 10 in fermentation 15h, subtilis D9 fermented liquid
8cfu ml
-1;
4) by vinegar grain: wheat bran 3:1 mixing in mass ratio, 120 DEG C of sterilizing 30min, obtained solid fermentation substratum; Obtained subtilis D9 fermented liquid is inoculated into solid fermentation substratum according to volume ratio 10% ratio, leavening temperature is 37 DEG C, and fermented incubation time is 5 days, within fermenting process every 10-20 hour, stirs once, fermentation ends cryodrying, obtains subtilis D9 solid fermentation bacterial classification;
5) trichoderma asperellum 12 solid fermentation bacterial classification and subtilis D9 solid fermentation bacterial classification are mixed according to 5:1 mass ratio, pulverize to obtain mixed bacterium powder with pulverizer;
6) by obtained mixed bacterium powder according to the volume ratio of compost 5-6% be inoculated into become thoroughly decomposed vinegar grain compost or digest process with the addition of in the compost of cow dung or pig manure, stirring and evenly mixing thoroughly, bar buttress formula is banked up fermentation, leavening temperature is 30-50 DEG C, turning in every 3 days 1 time in fermenting process, ferment and terminate after 15-20 days, trichoderma asperellum 12 content reaches 0.2 × 10
8cfu g
-1above, subtilis D9 content reaches 0.5 × 10
8cfu g
-1above, bacterial strain stable content, obtains saltings growth-promoting biological organic fertilizer.
The preparation method of described saltings growth-promoting biological organic fertilizer, it is characterized in that: measure with volume 1L, the compound method of PDA nutrient solution is: be cut into small pieces to be put in water after peeling with 100 g potatoes and boil, 30 min are boiled after boiling, 5g common sucrose and 2g rice straw powder is added after filtering in filtrate, be settled to 1000 ml, pH value nature, 115 DEG C of sterilizing 30 min.
The preparation method of described saltings growth-promoting biological organic fertilizer, is characterized in that: measure with volume 1L, and the compound method of beef soup peptone liquid fermentation medium is: extractum carnis 3g, peptone 10g, NaCl 3g, tap water 1000ml, pH scope 7.0-7.5,121 DEG C of sterilizing 20min.
The preparation method of described saltings growth-promoting biological organic fertilizer, is characterized in that: the compost that step 6) is used, quality of organic matter than content >=45%, total nutrient >=5%, water content mass ratio≤30%.
Above-mentioned saltings growth-promoting biological organic fertilizer can be used in the improvement of beach saline-alkali soil.
Beneficial effect: a kind of saltings provided by the invention growth-promoting biological organic fertilizer, produced by the bacterium and fungus twice fermentation agricultural solid residue with the effect of Promoting plant growth, microbial organic fertilizer of the present invention tool compared with product in the market has the following advantages: the functional microorganism 1) in this biological organic fertilizer is mainly derived from beach geavy salt soil and salinification continuous cropping soil, cucumber seeds root length wherein through trichoderma asperellum 12 fermentation liquor treatment is untreated 2.1 times, cucumber seeds root length through subtilis D9 fermentation liquor treatment is untreated 1.7 times, two strain bacterial strains can adapt to high salinity environment, be conducive to surely growing in salt affected soil, 2) carrier of this biological organic fertilizer use is for adding pig manure or the cow dung compost of rice husk in vinegar grain compost or digest process, due to beach saline-alkali wait salt affected soil easily harden, soil depletion, through the rice husk of the rotten process of heap, can effectively keep soil from packing together, surely growing for growth-promoting microorganism and grow provides enough air and heat, 3) due to be biological bacterial strain and cultural solid organic waste by certain explained hereafter, growth-promoting functional microorganism can be determined to grow for a long time in soil, and applied once is permanently effective, does not pollute, is conducive to the Sustainable Exploitation of beach saline-alkali soil.
Accompanying drawing explanation
Fig. 1 is the effectiveness comparison figure after the present invention and existing fertilizer process.
Embodiment
Below in conjunction with embodiment, the present invention is further described.
(1) microbial inoculum is produced
1) trichoderma asperellum 12 is inoculated into PDA nutrient solution, carry out liquid fermenting production, the condition of its fermentative production is: initial pH scope is 6.5-7.2, culture temperature 25-30 DEG C, dissolved oxygen air flow scope is 30-100%, 170rpm, fermentation later stage mycelium pellet is all broken into mycelia fragment, colony-forming unit>=1 × 10 of fermented liquid bacterial strain
8cfu ml
-1; PDA nutrient solution compound method used is (to prepare 1L substratum): be cut into small pieces to be put in water after peeling with 100 g potatoes and boil, boil 30 min after boiling, add 5g common sucrose and 2g rice straw powder after filtering in filtrate, be settled to 1000 ml, pH value nature, 115 DEG C of sterilizing 30 min.
2) be inoculated in solid fermentation matrix by trichoderma asperellum 12 fermented liquid, inoculum size is volume ratio 5%, ferments 6 days, oven drying at low temperature; Solid fermentation matrix used is vinegar grain: wheat bran mass ratio is 5:1,120 DEG C of sterilizing 30min;
3) subtilis D9 is inoculated into beef soup peptone liquid fermentation medium, produce subtilis D9 fermented liquid, the condition of its fermentative production is: initial pH scope is 6.5-7.2, culture temperature 35-37 DEG C, dissolved oxygen air flow scope is 30-100%, 120-180rpm, Number of spores>=2 × 10 in fermentation 15h, subtilis D9 fermented liquid
8cfu ml
-1; The compound method of beef soup peptone liquid fermentation medium used is prepare according to 1L substratum: extractum carnis 3g, peptone 10g, NaCl 3g, tap water 1000ml, pH scope 7.0-7.5,121 DEG C of sterilizing 20min;
4) obtained subtilis D9 fermented liquid is inoculated into solid fermentation substratum according to volume ratio 10% ratio, leavening temperature is 37 DEG C, fermented incubation time is 5 days, within in fermenting process every 10-20 hour, stir once, fermentation ends obtains subtilis D9 solid fermentation bacterial classification, cryodrying; Solid fermentation substratum used is vinegar grain: wheat bran mass ratio is 3:1,120 DEG C of sterilizing 30min.
5) trichoderma asperellum 12 solid fermentation bacterial classification and subtilis D9 solid fermentation bacterial classification are mixed thoroughly according to 5:1 mass ratio, pulverize to obtain mixed bacterium powder with pulverizer.
(2) biological organic fertilizer is produced
Be inoculated into according to the volume ratio of 5%-6% by mixed bacterium powder in the vinegar grain compost become thoroughly decomposed, stirring and evenly mixing thoroughly, bar buttress formula is banked up fermentation, leavening temperature is 30-50 DEG C, turning in every 3 days 1 time in fermenting process, ferment and terminate after 15-20 days, trichoderma asperellum 12 content reaches 0.2 × 10
8cfu g
-1above, subtilis D9 content reaches 0.5 × 10
8cfu g
-1above, bacterial strain stable content; Used become thoroughly decomposed vinegar grain compost, quality of organic matter than content>=45%, total nutrient>=5%, water content mass ratio≤30%.The goods obtained are saltings of the present invention growth-promoting biological organic fertilizer.
(3) mud-flat soil long-term cropping Biological control
Experiment soil Yancheng, Jiangsu Province Coastal beach soil, soil salt content 25 ‰, pH value 8.39.
Setup Experiments three process, is respectively: 1, control treatment, and 2, fertilizer process, 3, biological organic fertilizer process of the present invention.
Test, cyperus malaccensis fluffy by basin pot culture kind alkali and summer cypress, soil 5kg placed by every alms bowl, and the rate of fertilizer of fertilizer and biological organic fertilizer process is respectively the 5%(w/w of soil quality).Each process plants that alkali is fluffy respectively, cyperus malaccensis and summer cypress.Plant after 30 days, measure the biomass of the plant of each process.
Control treatment alkali is fluffy, cyperus malaccensis and summer cypress surviving rate are respectively 80%, 87% and 68%, and the surviving rate of fertilizer process 3 kind of plant is respectively 100%, 100% and 85%, and biological organic fertilizer process 3 kind of plant surviving rate all reaches 100%.The biomass of different treatment measures as shown in Figure 1, compared to control treatment, fertilizer process alkali is fluffy, cyperus malaccensis and summer cypress biomass add 9.1%, 15.6% and 11.9% respectively, and biological organic fertilizer process biomass adds 49.1%, 56.3% and 52.2% respectively.Biological organic fertilizer of the present invention can plant surviving and growing of salt-tolerant plant with promoting beach saline-alkali significantly.
The above is only the preferred embodiment of the present invention; be noted that for those skilled in the art; under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (7)
1. a saltings growth-promoting biological organic fertilizer, is characterized in that: described saltings growth-promoting biological organic fertilizer is for making by trichoderma asperellum 12 and subtilis D9 fermentation agricultural solid residue.
2. saltings according to claim 1 growth-promoting biological organic fertilizer, it is characterized in that: described trichoderma asperellum 12, on May 16th, 2013 is preserved in China typical culture collection center, and preservation address is Luo Jia Shan, wuchang, wuhan, and bacterial strain preserving number is CCTCC NO:M2013213; Described subtilis D9 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on May 16th, 2014, and preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, and bacterial strain preserving number is CGMCC NO.9170.
3. saltings according to claim 1 growth-promoting biological organic fertilizer, is characterized in that: in the growth-promoting biological organic fertilizer of described saltings, trichoderma asperellum 12 quantity>=0.2 × 10
8cfu g
-1, subtilis D9>=0.5 × 10
8cfu g
-1, pH value 5.5, water content mass ratio≤30%, quality of organic matter is than content>=45%.
4. the preparation method of the saltings growth-promoting biological organic fertilizer according to any one of claims 1 to 3, is characterized in that, comprise the following steps:
1) trichoderma asperellum 12 is inoculated into PDA nutrient solution, carry out liquid fermenting production, the condition of its fermentative production is: initial pH scope is 6.5-7.2, culture temperature 25-30 DEG C, dissolved oxygen air flow scope is 30-100%, 170rpm, fermentation later stage mycelium pellet is all broken into mycelia fragment and obtains trichoderma asperellum 12 fermented liquid, colony-forming unit>=1 × 10 of trichoderma asperellum 12 fermented liquid bacterial strain
8cfu ml
-1;
2) by vinegar grain: wheat bran 5:1 mixing in mass ratio, 120 DEG C of sterilizing 30min make solid fermentation matrix; Be inoculated in solid fermentation matrix by trichoderma asperellum 12 fermented liquid, the inoculum size of trichoderma asperellum 12 fermented liquid is the volume ratio of solid fermentation matrix 5%, ferments 6 days, and oven drying at low temperature obtains trichoderma asperellum 12 solid fermentation bacterial classification;
3) subtilis D9 is inoculated into beef soup peptone liquid fermentation medium, produce subtilis D9 fermented liquid, the condition of its fermentative production is: initial pH scope is 6.5-7.2, culture temperature 35-37 DEG C, dissolved oxygen air flow scope is 30-100%, 120-180rpm, Number of spores>=2 × 10 in fermentation 15h, subtilis D9 fermented liquid
8cfu ml
-1;
4) by vinegar grain: wheat bran 3:1 mixing in mass ratio, 120 DEG C of sterilizing 30min, obtained solid fermentation substratum; Obtained subtilis D9 fermented liquid is inoculated into solid fermentation substratum according to volume ratio 10% ratio, leavening temperature is 37 DEG C, and fermented incubation time is 5 days, within fermenting process every 10-20 hour, stirs once, fermentation ends cryodrying, obtains subtilis D9 solid fermentation bacterial classification;
5) trichoderma asperellum 12 solid fermentation bacterial classification and subtilis D9 solid fermentation bacterial classification are mixed according to 5:1 mass ratio, pulverize to obtain mixed bacterium powder with pulverizer;
6) by obtained mixed bacterium powder according to the volume ratio of compost 5-6% be inoculated into become thoroughly decomposed vinegar grain compost or digest process with the addition of in the compost of cow dung or pig manure, stirring and evenly mixing thoroughly, bar buttress formula is banked up fermentation, leavening temperature is 30-50 DEG C, turning in every 3 days 1 time in fermenting process, ferment and terminate after 15-20 days, trichoderma asperellum 12 content reaches 0.2 × 10
8cfu g
-1above, subtilis D9 content reaches 0.5 × 10
8cfu g
-1above, bacterial strain stable content, obtains saltings growth-promoting biological organic fertilizer.
5. the preparation method of saltings according to claim 4 growth-promoting biological organic fertilizer, it is characterized in that: measure with volume 1L, the compound method of PDA nutrient solution is: be cut into small pieces to be put in water after peeling with 100 g potatoes and boil, 30 min are boiled after boiling, 5g common sucrose and 2g rice straw powder is added after filtering in filtrate, be settled to 1000 ml, pH value nature, 115 DEG C of sterilizing 30 min.
6. the preparation method of saltings according to claim 4 growth-promoting biological organic fertilizer, it is characterized in that: measure with volume 1L, the compound method of beef soup peptone liquid fermentation medium is: extractum carnis 3g, peptone 10g, NaCl 3g, tap water 1000ml, pH scope 7.0-7.5,121 DEG C of sterilizing 20min.
7. the preparation method of saltings according to claim 4 growth-promoting biological organic fertilizer, is characterized in that: the compost that step 6) is used, quality of organic matter than content >=45%, total nutrient >=5%, water content mass ratio≤30%.
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| CN105481486A (en) * | 2016-01-04 | 2016-04-13 | 南京农业大学 | Method for producing trichoderma bio-organic fertilizer from straw and filtrated mud and obtained product |
| CN105819913A (en) * | 2016-03-14 | 2016-08-03 | 北京天工环境治理有限责任公司 | Composite bacterial fertilizer, preparation method and application thereof |
| CN106190138A (en) * | 2016-06-30 | 2016-12-07 | 山东胜伟园林科技有限公司 | A kind of anti-salt alkali composition containing Semen Strychni and preparation method thereof |
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| CN110603923A (en) * | 2019-09-25 | 2019-12-24 | 正大农业科技(浙江)有限公司 | Biological improvement method for saline-alkali soil |
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| CN111647527A (en) * | 2020-05-21 | 2020-09-11 | 浙江工业大学 | Method for improving saline-alkali soil and preserving fertilizer by utilizing bacillus subtilis |
| CN111876342A (en) * | 2020-06-19 | 2020-11-03 | 吉林地富肥业科技有限责任公司 | Low-temperature straw decomposing microbial inoculum and preparation method and application thereof |
| CN112746024A (en) * | 2021-01-19 | 2021-05-04 | 南京农业大学 | Trichoderma composite microbial agent and bio-organic fertilizer prepared from same |
| CN113214034A (en) * | 2021-05-24 | 2021-08-06 | 宁夏润地农牧科技有限公司 | Organic fertilizer applicable to sandy saline-alkali soil and preparation method thereof |
| CN114685201A (en) * | 2022-04-20 | 2022-07-01 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Method for changing substance conversion metabolism and pathogenic microorganism in compost fermentation |
| CN115011353A (en) * | 2022-06-28 | 2022-09-06 | 黑龙江八一农垦大学 | Application of trichoderma asperellum in repairing saline-alkali farmland soil and/or promoting plant growth |
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