CN106916756A - Fungal bacterial strain, microbial bacterial agent, stalk soil-repairing agent and its application - Google Patents
Fungal bacterial strain, microbial bacterial agent, stalk soil-repairing agent and its application Download PDFInfo
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Abstract
The present invention relates to technical field of microbe application, in particular to a kind of fungal bacterial strain, microbial bacterial agent, stalk soil-repairing agent and preparation method and application.The bacterial strain is trichoderma asperellum WL, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is：CGMCC NO.13766；The preservation time is：On 03 23rd, 2017.Described bacterial strain has the effect of enriching heavy metal material, and with cellulose family degradation function, can well be used for straw degradative and soil remediation.Microbial bacterial agent containing the bacterial strain more facilitates and effective in the application of straw degradative and follow-up soil.The stalk soil-repairing agent is obtained by above-mentioned microbial inoculum and stalk fermentation, not only overcome the difficulty that existing straw from village is dissolved, and turn waste into wealth, the discarded stalk in rural area becomes soil-repairing agent, saline and alkaline soil can be repaired, the heavy metal of contaminated land and the pollution sources of petrochemical industry class are removed, there is no the danger of any side effect and secondary pollution.
Description
Technical field
The present invention relates to technical field of microbe application, in particular to a kind of fungal bacterial strain, microbial bacterial agent, straw
Stalk soil-repairing agent and preparation method and application.
Background technology
Compared with atmosphere and water pollution, soil remediation seems more difficult.Ministry of Agriculture's rapid test paper heavy metal-polluted soil is dirty
Dye preventing and treating panel member, College of Resources and Environmental Sciences, China Agricultural University professor Su Dechun represent that not only soil pollution harm is tight
Weight, and it is different from some organic pollutions, and the heavy metal in soil cannot degrade, very big with soil separating difficulty, repair
It is extremely difficult.If heavy metal pollution of soil owner mercury, lead, copper, zinc, chromium, nickel, tin and metallic arsenic etc..At present, soil remediation
Technology has a lot, it may be said that be to cut both ways, from both at home and abroad it is various about Heavy Metal Pollution Control in terms of document report can be with
It was found that, its governance way mostly uses Physical, chemical method and bioanalysis.Physical method effect is good, is not limited by edaphic condition
System, but substantial amounts of human and material resources and financial resources are needed, and investment is big, it is slow to take effect, and can also destroy soil texture and the micro- life of soil
Thing；Chemical method instant effect, but often capital intensive, need to complicated appointed condition, upset soil layer construction and easily bring secondary
Pollution etc., is even more to the pollution of large area and has no way out, therefore, these methods can not all tackle the problem at its root.With people
To the pay attention to day by day of environmental protection, administered in the urgent need to finding one in the case where soil physical chemistry property is not destroyed
The new way of heavy-metal contaminated soil, thus biological prosthetic is prefered method.
Stalk treatment is the problem that current rural area is most had a headache, crop straw burning pollution environment after the annual harvest season, serious dirty
Dye air increases PM2.5, allows the flight cannot landing when serious.Based on this, the Chinese government encourages peasant's straw-returning, straw always
Bar also field is a traditional agricultural measures, and to improving soil organic matter content, preservation of fertility realizes the sustainable of agricultural
Development has great importance.In recent years, with mechanization of agriculture and the development of microbial technique (microorganism formulation), straw is straight
Connect also field and be commonly available attention, and large-scale popularization application in China.Correct for burning the wave of straw to a certain extent
Take phenomenon, reduce the destruction to atmospheric environment and ecological environment, while soil fertility of having fostered and apply fertilizer, effectively increases the soil organism and contain
Amount.
After the soil being contaminated is repaired, farming is often still cannot be used for due to the missing of soil with organic matter
Thing is grown, and substantial amounts of organic matter can be then produced after stalk is degraded by microorganisms, and is effective source of soil and fertilizer.Therefore, if
Can provide it is a kind of it is effective soil remediation is dissolved the technology that is combined with stalk, can undoubtedly play a multiplier effect.
In view of this, it is special to propose the present invention.
The content of the invention
The first object of the present invention is to provide a kind of fungal bacterial strain, and described bacterial strain has complexing enriching heavy metal material
Effect, and with cellulose family degradation function, can well be used for straw degradative and soil remediation.
The second object of the present invention is to provide a kind of microbial bacterial agent containing the bacterial strain, so that the bacterial strain is in straw
Stalk is degraded and the application of follow-up soil more facilitates and effective.
The third object of the present invention is to provide a kind of stalk soil-repairing agent, and the stalk soil-repairing agent not only overcomes
The difficulty that existing straw from village is dissolved, and turn waste into wealth, the discarded stalk in rural area becomes soil-repairing agent, can repair saline-alkali soil
Ground, removes the heavy metal of contaminated land and the pollution sources of petrochemical industry class, does not have the danger of any side effect and secondary pollution.
In order to realize above-mentioned purpose of the invention, spy uses following technical scheme：
One fungus strains, the bacterial strain is trichoderma asperellum (Trichoderma asperellum) WL, is preserved in China
Microbiological Culture Collection administration committee common micro-organisms center, deposit number is：CGMCC NO.13766；The preservation time is：
On 03 23rd, 2017.
The strain Classification system be Trichoderma asperellum, Chinese scientific name be trichoderma asperellum, the entitled WL of bacterial strain,
Obtained from the screening of heavy metal pollution area.By identification, the Pseudomonas is in Fungi Imperfecti door, hyphomycetales, fungus Trichoderma.
The biological property of the bacterial strain is：Bacterium colony 27~29 DEG C of cultures on PDA plate, expand to 10cm, initial stage for 3-4 days
Faint yellow sparse, mycelia forms bottle green Chan Bao areas, reverse side white afterwards in surface sprawl growth；Conidium is spherical, nearly ball
Shape, it is single near colourless, it is in pistac during aggregation, wall is smooth.Morphological feature meets Trichoderma asperellum feature.
Microbial bacterial agent containing described trichoderma asperellum WL or its spore fall within this application claims scope.
During practical application, it is contemplated that it may need the reasons such as transport, it is necessary to which its Amplification Culture is made into microbial bacterial agent
Form expanding its range of application.
Above-mentioned microbial bacteria microbial inoculum, preferably liquid bacterial agent, in the liquid bacterial agent, nutrient solution is preferably Liquid Culture
Base or the further nutrients such as addition glucose, thiamine, biotin and nicotinic acid.
Preferably, every milliliter of microbial inoculum contains 1 × 1010~12Trichoderma asperellum WL spores.
Preferably, microbial bacterial agent as described above, also contains 1 × 10 in the microbial bacterial agent10~12Stearothermophilus
Native bacillus work spore.
The Geobacillus stearothermophilus are bought in ThermoFisher, trade name Culti-LoopsTM stearothermophilus
Native bacillus12980TM, article No. is R4601219.The bacterium can be well matched with trichoderma asperellum WL and be fermented,
Increase fermentation efficiency.
Preferably, microbial bacterial agent as described above, the trichoderma asperellum WL lives with the Geobacillus stearothermophilus
The mixed proportion of spore is (5~10):(0~10).
The mixed proportion is in terms of live spore count or viable count.
Preferably, microbial bacterial agent as described above, it is characterised in that also contain plant breast bar in the microbial bacterial agent
It is bacterium, trichoderma reesei, De Shi lactobacillus lactis, methane is thermophilic micrococcus luteus, thermophilic metal fever anaerobic bacillus(cillus anaerobicus), Thermoactinomyces albus, withered
In careless bacillus, sporotrichum thermophile, bacillus licheniformis, bacillus stearothermophilus, Bacillus cereus, citric acid bacillus
One or more.
A kind of new method of stalk of dissolving, is dissolved using microbial bacterial agent as described above to stalk.
A kind of stalk soil-repairing agent, the stalk soil-repairing agent is mixed by microbial bacterial agent as described above with fermentation
Thing fermentation is obtained；
The fermenting mixture includes stalk, sludge and domestic organic garbage.
Sludge can provide necessary cultivation mechanism for the growth of microorganism, and with the function of holding moisture, organic rubbish
Rubbish can provide abundant carbon source and nutriment for microbial fermentation so that the microorganism in the microbial inoculum that the application is provided gives birth to rapidly
Logarithmic phase is grown to, to shorten fermentation time.
Preferably, stalk soil-repairing agent as described above, tells that stalk is particle diameter 1~5mm pulverized particles.
Preferably, stalk soil-repairing agent as described above, the mass ratio of the stalk, sludge and domestic organic garbage is
(5~8)：(1~5)：(1~5)；
Preferably, the water content of the fermenting mixture is 40%~60%.
Domestic organic garbage refers to the discarded object containing organic components in house refuse, including pericarp, dish skin leaf, leftovers
Dish, wood, waste paper, dish skin etc..
Application of the stalk soil-repairing agent in the in-situ immobilization of salt affected soil as described above.
Application of the stalk soil-repairing agent as described above in heavy metal-polluted soil reparation.
Compared with prior art, beneficial effects of the present invention are：
(1) turn waste into wealth.The problem of stalk treatment is solved, it is resource to become rubbish.Can also be by city when fermenting simultaneously
Organic waste twice laid；With production and application advantage：Small investment, energy consumption is low, wide material sources.
(2) soil heavy metal pollution and salt-soda soil are solved the problems, such as, while the excellent soil of soil remediation.Stalk soil is repaiied
Multiple agent contains substantial amounts of beneficial bacterium, and the various elements needed for plant growth, has adsorbed high-efficiency nitrogen-fixing bacterium, phosphorus-solubilizing bacteria, potassium decomposing
The biological organic and inorganic fertilizer material of the soil profitable strain such as bacterium, Antagonistic Fungi, the facilitation to crop growth is significantly better than now
Compost, be conducive to improve quality of agricultural product, increase economic efficiency.
(3) stalk soil-repairing agent raw material of the invention is cheap, process is simple, is suitable to heavy industrialization utilization, practical
Property is strong.
The trichoderma asperellum (Trichoderma asperellum) that the application is provided, the entitled WL of bacterial strain is preserved in China micro-
Biological inoculum preservation administration committee common micro-organisms center, preservation address is：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3,
Institute of Microorganism, Academia Sinica；The preservation time is：On 03 23rd, 2017, deposit number CGMCC NO.13766.Through protecting
Tibetan center was detected as survival strains on 03 23rd, 2017.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the present invention.It is unreceipted specific in embodiment
Condition person, the condition advised according to normal condition or manufacturer is carried out.Agents useful for same or the unreceipted production firm person of instrument, are
The conventional products for obtaining can be bought by city.
Embodiment 1
Microbial bacterial agent containing trichoderma asperellum conidia powder and preparation method thereof is present embodiments provided, the method is included such as
Lower step：
The strain of preservation is first connected on PDA culture medium flat board, in 28 DEG C of activation cultures to spore is produced, by the conidia powder of gained
Sieving (200 mesh), obtains the trichoderma asperellum conidia powder；
It is 1 × 10 to take conidia powder and be configured to concentration11The suspension of individual spore/mL, obtains microbial liquid microbial inoculum.
Wherein, the collocation method that the PDA culture medium addressed contains is：200g potatos are weighed, clean peeling is cut into small
Block, the 1000ml that adds water boils half an hour, filtered through gauze, then Jia 10~20g glucose, fully dissolving after constant volume to 1000ml,
121 degree of sterilizing 30min..
Embodiment 2
Microbial bacterial agent containing trichoderma asperellum conidia powder and preparation method thereof is present embodiments provided, the method is included such as
Lower step：
1), the strain of preservation is first connected on PDA culture medium flat board, in 28 DEG C of activation cultures to spore is produced, by the spore of gained
Sub- powder sieves (200 mesh), obtains the trichoderma asperellum conidia powder；
It is 1 × 10 to take conidia powder and be configured to concentration11The suspension of individual spore/mL, obtains the first microbial liquid microbial inoculum.
Wherein, the collocation method that the PDA culture medium addressed contains is：200g potatos are weighed, clean peeling is cut into small
Block, the 1000ml that adds water boils half an hour, filtered through gauze, then Jia 10~20g glucose, fully dissolving after constant volume to 1000ml,
121 degree of sterilizing 30min..
2), Geobacillus stearothermophilus by specification is recorded and cellar culture and is counted, subsequent solvent suspension, obtain the
Two microbial liquid microbial inoculums；
Wherein, the first microbial liquid microbial inoculum is with the solvent formula used by the second microbial liquid microbial inoculum：Quality hundred
The emulsifying agent GeronolVO/01 of fraction 8%~20%, 0.5%~5% sodium carboxymethylcellulose, 10%~15% lignin sulfonic acid
Calcium, 0.1%~3% butylated hydroxy anisole, 3%~10% organobentonite, 0.1%~0.6% dextrin, 0.1%~2% are prevented
Rotten agent and 24.4%~77.7% vegetable oil are constituted.
3), the first microbial liquid microbial inoculum is mixed with the second microbial liquid microbial inoculum, gained liquid bacterial agent is measured
In for trichoderma asperellum spore content be 1 × 1011Individual/ml.The trichoderma asperellum spore and the Geobacillus stearothermophilus
The mixed proportion of spore living is 8:5.
Embodiment 3
In the present embodiment, preparation manipulation is with embodiment 2, as different from Example 2, in the present embodiment, except addition
Outside Geobacillus stearothermophilus, also extra addition Lactobacillus plantarum, De Shi lactobacillus lactis, methane is thermophilic micrococcus luteus, white
Thermophilic actinomycete and citric acid bacillus, above-mentioned strain contain according to being added after the composite bacteria liquid for waiting the bacterium solution volume proportioning of ratio to constitute
Bacterium amount is 1-9 × 1010Individual/ml.
Embodiment 4
A kind of solid fungicide and preparation method thereof is present embodiments provided, following steps are specifically included：
The liquid bacterial agent that above-described embodiment 3 is made (is carried with 20 grams of talcum powder according to every 1 kilogram liquid microbial inoculum as solid
Body) ratio add be sufficiently stirred for into talcum powder mix, filtering, dry, crush, obtain final product solid fungicide.
Embodiment 5
In the present embodiment, with embodiment 4, as different from Example 4, in the present embodiment, used consolidates preparation manipulation
Body carrier is the mixture of milk powder, talcum powder, fine sand and clay equal proportion.
Embodiment 6
The preparation method of stalk soil-repairing agent is present embodiments provided, the method comprises the following steps：
By two-step microbial fermentation stalk is produced using stalk, sludge and domestic organic garbage and branch leaf soil is repaiied
Multiple agent, method is simple, with low cost.
1), pretreatment of raw material：
The impurity such as plastics, metal, bone in a, removal fermentation raw material；
B, stalk is entered disintegrating machine, after crushing, the particle diameter of raw material is in 1~5cm；
C, it is broken after stalk mix with other fermentation raw materials, the mixing quality ratio of stalk, sludge and domestic organic garbage
It is 6：3：3.The material obtained after mixing is entered into mixer, the adjustment that adds water and is stirred moisture content so that fermentation materials it is aqueous
Rate is 40%~60%.
2), obtained in step upwards in fermentation materials addition fermentation tank, add Anaerobic rumen microbial bacterial group and infusorian,
Stirring carries out pre fermentation.Temperature 25~35 DEG C of holding spontaneous fermentation 2~3 days, Rapid Fermentation after fermentation body is degenerated substantially, wood
Matter fiber is decomposed.Make temperature in tank rise to 70~80 DEG C to be kept for 2~3 days, kill grass seeds and harmful bacteria；Treat temperature drop in tank
To 30~35 DEG C, 5%~10% yeast, 5~12% silicate bacteria are added, stirred, control temperature at 30~35 DEG C, after
Supervention ferment 3~4 days；Use Filter Press；Gained filtrate can be used as can reuse fermentative microflora.Filter cake can be used as soil
Sill carries out adding strain fermentation in drying subsequent handling processed.
3), plus the microbial inoculum for preparing of embodiment 2 ferments 2~3 days again to top gained tunning, by microbial inoculum with it is upper
The fermentate that step is obtained is by weight 1:1000 ratio is well mixed.Short time temperature is increased at 55-85 DEG C simultaneously, is kept
Germ composition is killed again within 48 hours.
4), allow the product dehydration obtained after fermenting to dry, make moisture less than 15%, crude product is crushed to particle diameter 1
Packaging is made stalk soil-repairing agent after~2mm.
Experimental example 1
The stalk soil-repairing agent prepared using the embodiment of the present invention 6 carries out soil improvement experiment.
Plot A areas and Beijing are polluted to plant of Daxing district, Beijing, China using stalk soil-repairing agent proposed by the present invention
Sludge of sewage treatment plant heap carries out improved test.Concrete operations are：2 mu of wastelands are respectively chosen as experimental plot in two places and detect soil
Earth content of beary metal.In October, 2014 applies product of the present invention, after turn over flood irrigation, June 5 was sowed, and crop is eggplant.Broadcast
Plant preceding soil sampling on the two carries out nutrient detection again, and sowing carries out emergence rate measure two weeks after.What test plot 1~3 was applied divides
Not Wei the soil conditioner that provides of embodiment 2~4, its application process uniformly spread fertilizer over the fields in soil conditioner disposably to spread fertilizer over the fields
Top layer, plowed soils 0cm~20cm, make soil-repairing agent be well mixed with soil, every mu of 300 kilograms of consumption.
Through statistics, plant of Daxing district, Beijing, China pollution plot A areas eggplant emergence rate is 92%, sewage treatment plant of Beijing
The eggplant emergence rate of sludge is 93%.Eggplant yield also can reach the 80%~90% of normal soil harvest.
And if in normal Planting Crops, because soil-repairing agent that the application is provided is by fermentation after stalk
Arrive, therefore itself also can be used as Fertilizer application.The stalk soil-repairing agent that directly applying embodiment 6 is prepared also has very
Obvious production-increasing function, the yield of corn can be dramatically increased using soil-repairing agent, as corn base manure, specific yield highest,
Yield per mu reaches 559.04kg/ mus, than traditional 461.13kg/ mus of volume increase of administration azophoska cultural method per mu yield
21.23%.From the point of view of full grains degree, mass of 1000 kernel reaches 434.62g, than the cultural method of traditional administration azophoska
379.26g increased 14.59%.From the point of view of plant height, difference is not notable.
Although illustrate and describing the present invention with specific embodiment, but will be appreciated that without departing substantially from of the invention
Many other changes and modification can be made in the case of spirit and scope.It is, therefore, intended that in the following claims
Including belonging to all such changes and modifications in the scope of the invention.
Claims (10)
1. fungus strains, it is characterised in that the bacterial strain is trichoderma asperellum (Trichoderma asperellum) WL, is protected
China Committee for Culture Collection of Microorganisms's common micro-organisms center is hidden in, deposit number is：CGMCC NO.13766；Preservation
Time is：On 03 23rd, 2017.
2. the microbial bacterial agent of bacterial strain described in claim 1 is contained；
Preferably, every milliliter of microbial inoculum contains 1 × 1010~12Trichoderma asperellum WL spores.
3. microbial bacterial agent according to claim 2, it is characterised in that also contain 1 in every milliliter of microbial bacterial agent
×1010~12Geobacillus stearothermophilus live spore；
Preferably, the trichoderma asperellum WL and the mixed proportion of Geobacillus stearothermophilus work spore are (5~10):(0
~10).
4. the microbial bacterial agent according to Claims 2 or 3, it is characterised in that also contain plant in the microbial bacterial agent
Lactobacillus, trichoderma reesei, De Shi lactobacillus lactis, methane is thermophilic micrococcus luteus, thermophilic metal fever anaerobic bacillus(cillus anaerobicus), white high temperature unwrapping wire
Bacterium, bacillus subtilis, sporotrichum thermophile, bacillus licheniformis, bacillus stearothermophilus, Bacillus cereus, citric acid
One or more in bacillus.
5. a kind of new method of stalk of dissolving, it is characterised in that the microbial bacterial agent described in usage right 2~4 any one of requirement
Stalk is dissolved.
6. a kind of stalk soil-repairing agent, it is characterised in that the stalk soil-repairing agent is by any one of claim 2~4 institute
The microbial bacterial agent stated is obtained with fermenting mixture fermentation；
The fermenting mixture includes stalk, sludge and domestic organic garbage.
7. a stalk soil-repairing agent according to claim 6, it is characterised in that tell stalk for 1~5mm of particle diameter is crushed
Grain.
8. stalk soil-repairing agent according to claim 6, it is characterised in that the stalk, sludge and organic rubbish of living
The mass ratio of rubbish is (5~8)：(1~5)：(1~5)；
Preferably, the water content of the fermenting mixture is 40%~60%.
9. application of the stalk soil-repairing agent described in any one of claim 6~8 in the in-situ immobilization of salt affected soil.
10. application of the stalk soil-repairing agent described in any one of claim 6~8 in heavy metal-polluted soil reparation.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108277166A (en) * | 2018-03-27 | 2018-07-13 | 山东农业大学 | One plant of trichoderma asperellum and its application in lead-contaminated soil reparation |
| CN108949587A (en) * | 2018-08-14 | 2018-12-07 | 北京绿安创华环保科技有限公司 | Microbial bacterial agent, organic waste prone soil renovation agent and the preparation method and application thereof |
| CN109160861A (en) * | 2018-10-31 | 2019-01-08 | 周口师范学院 | A kind of simple trichoderma-straw bacterial manure preparation method |
| CN115011353A (en) * | 2022-06-28 | 2022-09-06 | 黑龙江八一农垦大学 | Application of trichoderma asperellum in repairing saline-alkali farmland soil and/or promoting plant growth |
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| CN109160861A (en) * | 2018-10-31 | 2019-01-08 | 周口师范学院 | A kind of simple trichoderma-straw bacterial manure preparation method |
| CN115011353A (en) * | 2022-06-28 | 2022-09-06 | 黑龙江八一农垦大学 | Application of trichoderma asperellum in repairing saline-alkali farmland soil and/or promoting plant growth |
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