CN108624539B

CN108624539B - Straw and cow dung ultrahigh-temperature decomposition agent and preparation and application thereof

Info

Publication number: CN108624539B
Application number: CN201810586225.8A
Authority: CN
Inventors: 王松艳; 刘辉; 周天惠; 王灵敏; 田丽君; 戎新克; 赵峰; 张彤; 黄卫; 连振鹏; 姜利杰
Original assignee: Hebei Rimidori Technology Co ltd
Current assignee: Hebei Rimidori Technology Co ltd
Priority date: 2018-06-08
Filing date: 2018-06-08
Publication date: 2021-10-08
Anticipated expiration: 2038-06-08
Also published as: CN108624539A

Abstract

The invention discloses a straw and cow dung ultrahigh-temperature decomposing inoculant and a preparation method and application thereof, and belongs to the field of decomposing inoculant. The decomposing inoculant is obtained by mixing, fermenting and drying straw, cow dung and a decomposing composite inoculant, wherein the decomposing composite inoculant comprises 1-10 parts of a thermophilic bacteria inoculant, 1-6 parts of a bacillus licheniformis inoculant, 1-6 parts of an aspergillus oryzae inoculant, 1-6 parts of a lactobacillus plantarum inoculant, 1-6 parts of a sugarcane orchid inoculant and 1-6 parts of a cold-resistant brevibacterium inoculant. Aiming at the problem of fermentation of the existing complex microbial inoculum: the compost has the advantages of slow decomposition and temperature rise, low fermentation temperature, long fermentation time, incomplete decomposition, poor compost quality and the like, and provides the decomposition agent with fast decomposition and temperature rise, fast temperature rise, high fermentation temperature, short fermentation time, more thorough decomposition and higher quality, and the composting method and the application thereof.

Description

Straw and cow dung ultrahigh-temperature decomposition agent and preparation and application thereof

Technical Field

The invention belongs to the field of a decomposing inoculant, and particularly relates to a straw and cow dung ultrahigh-temperature decomposing inoculant as well as a preparation method and an application thereof.

Background

With the development of agriculture and animal husbandry, the treatment of crop straws and animal wastes becomes a big problem, and once the treatment is not good, serious harm is caused to the survival and development of human beings, and great burden is brought to the environment. However, from the perspective of resource recycling, the straw and animal waste contain complex components, such as organic substances and inorganic salts, such as sugars, lipids, proteins, nitrogen, phosphorus, potassium, magnesium, sulfur, etc., and can be changed into valuable substances after treatment, and microbial fermentation is the most common method at present. As the main components of crop straws and cow dung are cellulose and substances which are difficult to decompose, the traditional natural fermentation method is slow in temperature rise, incomplete in decomposition and long in fermentation period, so that the efficiency is low, and secondary pollution can be caused to the environment. At present, the patents on microbial decomposing inocula are more:

chinese patent CN103614326B discloses a microbial inoculum with the highest fermentation temperature of 50-60 ℃, the microbial inoculum comprises the following components in percentage by weight (3-5): (1-2): (1-2): (1-2): (1-2), although the invention saves energy and reduces the loss of nitrogen, the fermentation temperature is lower, and the invention has certain influence on the degree of decomposition.

Chinese patent CN103642701B discloses a microbial inoculum with the highest fermentation temperature of 50-60 ℃, which is prepared from 5-7 parts of long spiny hair shell freeze-dried strain powder, 4-6 parts of trichoderma koningii freeze-dried strain powder, 3-5 parts of round fermentation hair shell freeze-dried strain powder, 3-5 parts of trichoderma viride freeze-dried strain powder and 2-3 parts of geotrichum candidum freeze-dried strain powder.

Chinese patent CN102660479B discloses a microbial inoculum with the highest fermentation temperature of 75 ℃, the microbial inoculum consists of 14 strains, the microbial inoculum can increase the total number of viable bacteria of products, and is beneficial to improving the maturity of materials, but the microbial inoculum has a long maturity period, and approximately takes 36 days to finish maturity, thereby greatly reducing the maturity efficiency.

Chinese patent CN1460664A discloses a microbial inoculum with the highest fermentation temperature of 70-90 ℃, which consists of 4-8 strains of thermophilic lignocellulose degrading bacteria, the invention promotes the compost maturity by the synergistic action of high-temperature bacteria and mesophilic bacteria, improves the product maturity quality, but in the primary fermentation stage, in order to ensure that the microorganism in the composite microbial inoculum 1 keeps activity and better plays a degrading function, the highest temperature must be controlled below 75 ℃, and the standard is difficult to meet in the production process.

The above-mentioned patent microbial inoculum is of many kinds, and the fermentation temperature diverse that can reach almost all is the medium temperature fungus, and it is slow to rise the temperature, and the fermentation temperature is low to lead to the ferment to degrade thoroughly, and miscellaneous fungus, worm's ovum and virus are more in its ferment, because the fermentation temperature is lower can not make it lethal, therefore the ferment is applied to fertilizer and can become the main pollution sources of seedling, wood, soil infection plant diseases and insect pests, and it can all cause the injury to seedling, wood, soil to be applied to orchard and field. The temperature of the fermentation product is quickly raised by selecting a proper microbial inoculum, the high-quality decomposition requirement is met, the fermentation is quickly decomposed, and the shortening of the fermentation time is very important.

Disclosure of Invention

The invention solves the problem that the existing complex microbial inoculum is fermented: the composting agent has the advantages of slow composting temperature rise, low fermentation temperature, long fermentation time, incomplete composting, poor composting quality, difficulty in meeting the quality requirements of people for using fermented composts and the like, and provides the composting agent with the advantages of fast composting temperature rise, fast temperature rise, high fermentation temperature, short fermentation time, complete composting and higher quality, and the composting method and the application thereof.

In order to achieve the above purpose, the following experimental scheme is adopted in the invention:

the utility model provides a straw and cow dung ultra-temperature decomposition agent, is dried after mixing fermentation by straw, cow dung and the compound microbial inoculum of becoming thoroughly decomposed and obtains its characterized in that: the decomposed complex microbial inoculum is prepared from the following raw materials in parts by weight: 1-10 parts of a thermophilic bacteria agent, 1-6 parts of a bacillus licheniformis agent, 1-6 parts of an aspergillus oryzae agent, 1-6 parts of a lactobacillus plantarum agent, 1-6 parts of a lancina sacchari agent and 1-6 parts of a cold-resistant brevibacterium agent.

Preferably, the decomposed complex microbial inoculum mainly comprises the following raw materials in parts by weight:

3-8 parts of a thermophilic bacteria agent, 2-5 parts of a bacillus licheniformis agent, 2-5 parts of an aspergillus oryzae agent, 2-5 parts of a lactobacillus plantarum agent, 2-5 parts of a sugarcane orchid bacterium agent and 2-5 parts of a cold-resistant brevibacterium agent;

more preferably, the decomposed complex microbial inoculum mainly comprises the following raw materials in parts by weight:

6 parts of a thermophilic bacteria agent, 4 parts of a bacillus licheniformis agent, 4 parts of an aspergillus oryzae agent, 4 parts of a lactobacillus plantarum agent, 4 parts of a lancina sacchari agent and 4 parts of a cold-resistant brevibacterium agent;

preferably, the thermophilic bacterium is a thermophilic bacterium (Thermalobacter composition) GW with the preservation number of CGMCC No. 15721;

preferably, the preparation method of the thermophilic bacteria agent comprises the following steps: inoculating thermophilic bacteria into high-temperature bacteria seed solid slant culture medium A, culturing at 60-90 deg.C for 18-22 hr, eluting to obtain eluted bacterial liquid, and adjusting bacterial concentration of the bacterial liquid to 4-6 × 10¹¹cfu/mL, inoculating 50-150mL/kg culture medium into solid culture medium B, mixing, standing at 60-90 deg.C for 16-20 hr, supplementing water for 1-3 times/day, drying, pulverizingObtaining the thermophilic bacteria agent with the bacteria agent content more than or equal to 6 multiplied by 10¹¹cfu/g。

More preferably, the thermophilic bacteria are dried for 0.5-1.5 days at 70-85 ℃ after fermentation culture, and are crushed by a crusher and sieved by a sieve with 30-50 meshes to obtain the thermophilic bacteria agent.

More preferably, the content of the thermophilic bacteria agent is 6-7 multiplied by 10¹¹cfu/g。

Further, the thermophilic bacteria seed solid culture medium A comprises the following components: 2-3g/L of yeast extract, 2-3g/L of bacterial peptone, 2-3g/L of glucose, 6-8g/L of PIPES, 8-12g/L of plant gel, 8-12g/L of agar powder and the balance of water, adjusting the pH value to 7.2-7.4, and sterilizing at 121 ℃ for 30 min.

Further, the solid medium B consists of: 100 portions of rice husk-containing material, 110 portions of bran-containing material, 100 portions of corn flour-containing material, 115 portions of bean cake powder, 100 portions of glucose-containing material, 2-3 portions of calcium sulfate, 2-4 portions of calcium oxide, 1-3 portions of dipotassium hydrogen phosphate and 0.5-1.2 portions of magnesium sulfate, wherein the weight ratio of the total amount of solid materials to water is as follows: 5-6:4-5 adding water into the solid material, and sterilizing at 121 deg.C for 45 min.

Preferably, the bacillus licheniformis is any one or more of bacillus licheniformis CICC10037, bacillus licheniformis CICC10084, bacillus licheniformis CICC10085 and bacillus licheniformis CICC 10092.

More preferably, the bacillus licheniformis is bacillus licheniformis cic 10037.

Preferably, the preparation method of the bacillus licheniformis agent comprises the following steps: inoculating Bacillus licheniformis into NA seed solid culture medium C, standing at 30-40 deg.C for 1-2d, eluting to obtain eluate, and adjusting the concentration of the eluate to 3-5 × 10¹⁰cfu/mL, inoculating the solid culture medium D according to the inoculation amount of 50-120mL/kg culture medium, stirring and mixing, standing and culturing at 30-40 ℃ for 1-2D, drying and crushing to obtain the bacillus licheniformis microbial inoculum, wherein the microbial inoculum content is more than or equal to 4 multiplied by 10¹⁰cfu/g；

More preferably, after the bacillus licheniformis is fermented and cultured, the bacillus licheniformis is dried for 3-4 days at the temperature of 30-40 ℃, crushed by a crusher and sieved by a sieve with 30-50 meshes to obtain the bacillus licheniformis microbial inoculum.

More preferably, the content of the bacillus licheniformis agent is 4-5 multiplied by 10¹⁰cfu/g；

Further, the composition of the NA seed solid medium C is as follows: 10-12g/L of peptone, 3-4g/L of beef powder, 10-12g/L of sodium chloride, 18-20g/L of agar and the balance of water, adjusting the pH value to 7.0-7.2, and sterilizing at 121 ℃ for 30 min;

further, the composition of the solid medium D is: 275 parts of 250-containing rice husk, 750 parts of 500-containing bran, 625 parts of 375-containing corn flour, 275 parts of 200-containing bean cake powder, 250 parts of 225-containing starch, 275 parts of 250-containing red soil, 50-62 parts of glucose, 12-15 parts of calcium sulfate, 15-20 parts of calcium oxide, 5-10 parts of dipotassium hydrogen phosphate and 5-6 parts of magnesium sulfate, wherein water is added into the solid materials according to the weight ratio of the total amount of the solid materials to the water of 7-15:2-5 for mixing, and the solid materials are sterilized at the temperature of 121 ℃ for 45 min;

preferably, the aspergillus oryzae is any one or more of aspergillus oryzae CICC2001, aspergillus oryzae CICC2011, aspergillus oryzae CICC2016 and aspergillus oryzae CICC 2022.

More preferably, the Aspergillus oryzae is Aspergillus oryzae CICC 2001;

preferably, the preparation method of the aspergillus oryzae microbial inoculum comprises the following steps: inoculating Aspergillus oryzae strain in PDA solid culture medium, standing at 25-30 deg.C for 5-6d, eluting to obtain eluate, and adjusting the concentration of the eluate to 2-4 × 10¹¹cfu/mL, inoculating in solid culture medium B according to inoculum size of 50-120mL/kg, standing at 25-30 deg.C for 5-6d, drying, and pulverizing to obtain Aspergillus oryzae viable bacteria agent with bacterium content of not less than 8 × 10¹⁰cfu/g。

More preferably, the aspergillus oryzae is dried for 3-5 days at 25-30 ℃ after being fermented and cultured, and is crushed by a crusher and sieved by a 30-50-mesh sieve to obtain the bacillus licheniformis agent.

More preferably, the content of the Aspergillus oryzae microbial inoculum is 8-10 multiplied by 10¹⁰cfu/g。

Further, the PDA solid medium comprises the following components: potato 200-250g/L, glucose 20-25g/L, agar 20-22g/L and water in balance, the pH is natural, and the sterilization is carried out for 30min at 121 ℃.

Preferably, the lactobacillus plantarum is lactobacillus plantarum CGMCC 1.2437.

Preferably, the preparation method of the lactobacillus plantarum microbial inoculum comprises the following steps: inoculating Lactobacillus plantarum in seed solid culture medium C, standing at 30-40 deg.C for 2-3d, eluting to obtain eluate, and adjusting concentration to 4-5 × 10¹⁰cfu/mL, inoculating the bacterial liquid into a solid culture medium F according to the inoculation amount of 50-120mL/kg culture medium, standing and culturing for 2-3d at 30-40 ℃, drying and crushing to obtain the lactobacillus plantarum microbial inoculum, wherein the microbial inoculum content is more than or equal to 8 multiplied by 10¹⁰cfu/g。

More preferably, the lactobacillus plantarum is dried for 2-3 days at the temperature of 30-40 ℃ after being subjected to fermentation culture, crushed by a crusher and sieved by a 30-50-mesh sieve to obtain the lactobacillus plantarum microbial inoculum.

More preferably, the content of the lactobacillus plantarum microbial inoculum is 8-9 multiplied by 10¹⁰cfu/g。

Further, the preparation method of the solid medium F comprises the following steps: 500 parts of bean cake powder 300-.

Preferably, the Hibiscus saccharensis is specifically Hibiscus sacchari (Laceyella sacchari) CP with the preservation number of CGMCC No. 13928;

preferably, the preparation method of the Langeria sacchari agent comprises the following steps: inoculating Hill-minck Saccharum in NA seed solid culture medium C, standing at 50-70 deg.C for 1-2d, eluting thallus to obtain eluate, and adjusting concentration to 5-5.5 × 10¹¹cfu/mL, inoculating the bacterial liquid into a solid culture medium A according to the inoculation amount of 50-120mL/kg culture medium, standing and culturing at 50-70 ℃ for 4-5d, drying and crushing to obtain the Living bacterial agent of the sugarcane orchid bacterium, wherein the content of the bacterial agent is not less than 7 x 10¹¹cfu/g；

More preferably, the Hizikia saccharatum is dried for 2-4 days at 50-70 ℃ after fermentation culture, crushed by a crusher and sieved by a sieve with 30-50 meshes to obtain the Hizikia saccharatum microbial inoculum.

More preferably, the content of the Hispania saccharina microbial inoculum is 7-8 multiplied by 10¹¹cfu/g；

Preferably, the Brevibacterium fritolerans is Brevibacterium fritolerans (Brevibacterium fritolerans) NH with the preservation number of CGMCC No. 15722;

preferably, the preparation method of the cold-resistant brevibacterium sp agent comprises the following steps: inoculating Brevibacterium frigidum in NA seed solid culture medium C, standing at 8-10 deg.C for 2-3d, eluting to obtain eluate, and adjusting concentration to 8-9 × 10¹⁰cfu/mL, inoculating the bacterial liquid into a solid culture medium G according to the inoculum size of 50-110mL/kg, standing and culturing at 8-10 ℃ for 3-4d, drying and crushing to obtain the brevibacterium frigosum bacterial agent, wherein the bacterial agent content is more than or equal to 3 multiplied by 10¹¹cfu/g；

More preferably, the brevibacterium frigostabile is dried for 7-8 days at the temperature of 8-10 ℃ after being fermented and cultured, and is crushed by a crusher and sieved by a sieve with 30-50 meshes to obtain the sugarcane orchid bacterium agent.

More preferably, the content of the brevibacterium fritolerans microbial inoculum is 3-5 multiplied by 10¹¹cfu/g；

Further, the composition of the solid medium G is: 620g/L of bran 600-.

The invention also aims to provide a preparation method of the straw and cow dung ultrahigh-temperature decomposing inoculant, which comprises the following steps:

(1) and (3) mixing the microbial inoculum: mixing a thermophilic bacteria agent, a bacillus licheniformis agent, an aspergillus oryzae agent, a lactobacillus plantarum agent, a sugarcane orchid bacterium agent and a cold-resistant brevibacterium agent according to the weight parts, and sieving by a sieve of 30-40 meshes to obtain a decomposed composite microbial agent;

(2) fresh straws and cow dung are mixed according to the mass ratio of 10-20: 1-5, mixing to obtain a mixed pile body, wherein the water content of the mixed pile body is 55% -65%;

(3) inoculating the decomposed complex microbial inoculum into a mixed compost body according to the inoculation amount of 1-2 per mill of the mass ratio, mixing and stirring, carrying out decomposition treatment, and composting for 10-15d to obtain the straw and cow dung ultrahigh-temperature decomposing inoculant.

The invention also aims to provide the application of the straw and cow dung ultrahigh-temperature decomposition agent in the field of fruit and vegetable planting.

Preferably, the straw and cow dung ultrahigh-temperature decomposition agent is used in rape planting.

More preferably, the straw and cow dung ultrahigh-temperature decomposition agent is used in rape planting, and the steps are as follows: adding the high-temperature decomposition agent into soil according to the proportion of 1% by mass, then sowing and planting, and maturing after rape is sowed for 50 days.

The strain source is as follows:

1. the thermophilic bacteria (Thermalobacter compositi) GW is obtained by separating, purifying and screening from the fermentation compost of the Qinhuang island. The strain is preserved in China general microbiological culture Collection center (CGMCC for short, with the address of 100101, China academy of sciences, institute No. 3, West Lu No.1, Kyoho, Beijing city, Chaoyang, and the postal code is CGMCC No.15721, which is classified and named as thermophilic bacteria (Thermaerobacter comporti) in 2018, 5 months and 2 days.

The thermophilic bacteria (Thermaerobacter composti) can grow in the range of 50-100 ℃, and the optimal growth temperature is 80 ℃;

the thermophilic bacteria (Thermaerobacter composi) can grow well when being coated on a thermophilic bacteria flat plate and cultured in a liquid thermophilic bacteria culture medium test tube at 80 ℃ after being cultured in thermophilic bacteria liquid culture medium with different pH values of 3-11, and the activity content of detected bacteria is 4-6 multiplied by 10¹¹cfu/g, and two experiments prove that the strain can tolerate the pH value in a range of 3-11, is relatively wide and has relatively strong acid-base tolerance.

2. The bacillus licheniformis CICC10037, the bacillus licheniformis CICC10084, the bacillus licheniformis CICC10085 and the bacillus licheniformis CICC10092 are purchased from China center for culture collection of industrial microorganisms. The growth temperature of the bacillus licheniformis selected by the invention is between 37 and 50 ℃, and the bacillus licheniformis can exist in a spore form, thereby resisting the severe environment. Meanwhile, the antibacterial agent can generate an anti-active substance, has a unique biological oxygen-deprivation action mechanism, and can inhibit the growth and reproduction of pathogenic bacteria.

3. The aspergillus oryzae CICC2001, the aspergillus oryzae CICC2011, the aspergillus oryzae CICC2016 and the aspergillus oryzae CICC2022 are purchased from China industrial microorganism strain preservation management center. The growth temperature of the selected aspergillus oryzae is 28 ℃, amylase, glucoamylase, cellulase and the like can be produced besides protease, and cellulose, starch, protein and polysaccharide substances in straws and cow dung can be effectively degraded, so that the aspergillus oryzae can be effectively converted into fertilizers.

4. The lactobacillus plantarum is lactobacillus plantarum CGMCC1.2437 and is purchased from China general microbiological culture Collection center. The growth temperature of the lactobacillus plantarum used in the invention is 30-35 ℃, and the lactobacillus plantarum is a natural preservative and can effectively reduce the yield of ammonia nitrogen and nitrite in the process of decomposition.

5. The cyanobacteria CP is obtained by separating, purifying and screening cow dung high-temperature decomposed products in Qinhuang island city in Hebei province, the preservation number is CGMCC No.13928, and the cyanobacteria CP is named as cyanobacteria Lanceflla sacchara (Laceyella sacchara) in classification, and the cyanobacteria CP is disclosed in the invention patent with the publication number of CN106916772A and the invention name of acid and alkali resistant and alcohol resistant cyanobacteria capable of quickly decomposing vinasse and application thereof.

The growth temperature of the sugarcane Hibescus CP is 30-75 ℃, the sugarcane Hibescus CP has the characteristics of high temperature resistance, alcohol resistance and acid and alkali resistance, can generate various degrading enzymes such as protease, lipase and the like, has the tolerance pH value range of 3-12, has the tolerance alcoholic strength range of 1-13%, not only can effectively improve the adaptability of the fertilizer to different acid-base soils, but also has obvious tolerance to the production of an alcohol fermentation process in the fertilizer fermentation process.

6. The Brevibacterium frigoritolerans is specifically Brevibacterium frigoritolerans (Brevibacterium frigoritolerans) NH which is obtained by laboratory separation, the strain is preserved in the general microorganism center (CGMCC for short, the address is microorganism institute of China academy of sciences, No. 3, national institute of sciences, No.1, northwest way of sunward, north district, Beijing city, the postal code is 100101), the preservation number is CGMCC No.15722, and the Brevibacterium frigoritolerans (Brevibacterium frigoritolerans) is classified and named.

Has the advantages that:

1. according to the straw and cow dung ultra-high temperature decomposition agent prepared by the invention, according to the characteristics of large temperature difference in different regions, straw and cow dung composition structure and nutritional ingredients, strains which can bear low temperature, medium temperature, high temperature and decompose various ingredients are scientifically compounded, and the mutual synergistic effect of different decomposition microbial agents is utilized to fully degrade the cellulose, lignin, protein, starch, fat and other ingredients in the straw and cow dung, so that the degradation capability of the decomposition agent on the straw and cow dung is effectively improved.

2. The brevibacterium frigostabile and the thermophilic bacterium have obvious low temperature resistance and high temperature resistance.

The cold-resistant brevibacterium matches thermophilic bacteria to enable the high-temperature decomposing agent to be rapidly fermented and heated in a place with a low northern temperature by virtue of the characteristics of high and low temperature resistance and rapid heating and fermentation of the brevibacterium, so that the time for fermentation to enter a high-temperature period is greatly shortened (1d reaches 60 ℃ and 3d reaches 98 ℃), the thermophilic bacteria can survive in an ultrahigh-temperature environment, the condition that all strains with overhigh temperature die are not completely degraded is avoided, the material decomposition degree is greatly improved, the time for maintaining high-temperature fermentation is long (85-98 ℃ is maintained for 3d), the fermentation period is shortened (only 15d), the yield and the quality of the organic fertilizer are effectively improved, secondary pollution caused by incomplete decomposition of straws and cow dung is prevented, and waste of manpower, material resources, financial resources and time is provided for preparing the high-quality organic fertilizer by high-temperature decomposition of the straws and cow dung.

3. Thermophilic bacteria have obvious strain activity, can quickly complete fermentation, saves the time and cost for preparing microbial inoculum, is inoculated in a high-temperature bacteria seed solid slant culture medium A, cultured for 18-22h at 60-90 ℃, the thalli of the thermophilic bacteria can be fully paved on the slant, and inoculated in a solid culture medium B, and statically cultured for 16-20h at 60-90 ℃, thus completing the fermentation.

4. The bacillus licheniformis (bacillus licheniformis CICC10037, bacillus licheniformis CICC10084, bacillus licheniformis CICC10085 and bacillus licheniformis CICC 10092) disclosed by the invention can exist in a spore form, so that the bacillus licheniformis resists a severe environment. Can produce active resisting matter, has unique biological oxygen-taking action mechanism and can inhibit the growth and propagation of pathogenic bacteria.

5. The aspergillus oryzae disclosed by the invention can produce amylase, glucoamylase, cellulase and the like besides protease, and can effectively degrade cellulose, starch, protein and polysaccharide substances in straws and cow dung.

6. The lactobacillus plantarum disclosed by the invention is a natural preservative, and the yield of ammonia nitrogen and nitrite in the decomposing process is remarkably reduced.

7. The sugarcane Hill-bacteriaceae disclosed by the invention has the characteristics of high temperature resistance, alcohol resistance and acid and alkali resistance, can generate various degrading enzymes such as protease and lipase, and has the tolerance pH value range of 3-12 and the tolerance alcoholic strength range of 1-13%.

Drawings

FIG. 1 is a graph showing temperature changes in different decomposition treatments during composting of straw and cow dung;

FIG. 2 is a graph showing changes in pH values under different composting processes in the composting process of straw and cow dung;

FIG. 3 is the change of organic matter content under different decomposition treatments in the straw and cow dung composting process;

FIG. 4 is the change of total nitrogen content under different decomposition treatments in the straw and cow dung composting process;

FIG. 5 shows the variation of water content under different composting processes during the composting process of straw and cow dung.

Detailed Description

Example 1 preparation method of straw and cow dung ultrahigh-temperature decomposing inoculant

(1) And (3) mixing the microbial inoculum: mixing 6 parts of a thermophilic bacteria agent, 4 parts of a bacillus licheniformis agent, 4 parts of an aspergillus oryzae agent, 4 parts of a lactobacillus plantarum agent, 4 parts of a lancina sacchari agent and 4 parts of a cold-resistant brevibacterium agent, and sieving by a 40-mesh sieve to obtain a thoroughly decomposed composite microbial agent;

(2) mixing fresh straws and cow dung according to a mass ratio of 5:1 to obtain a mixed pile body, wherein the water content of the mixed pile body is 55-65%;

(3) inoculating the decomposed complex microbial inoculum into a mixed compost body according to the inoculation amount of 1 per mill of the mass ratio, mixing and stirring, carrying out decomposition treatment, and composting for 13d at the initial composting temperature of 10 ℃ to obtain the ultrahigh-temperature decomposing agent for straw and cow dung.

The preparation method of the thermophilic bacteria agent comprises the following steps: inoculating thermophilic bacteria slant strain to high-temperature bacteria seed solid culture medium A, culturing at 80 deg.C for 18 hr, eluting thallus with sterile water, and adjusting thallus concentration to 4 × 10¹¹cfu/mL, absorbing 100mL of bacterial liquid, inoculating into 1kg of solid culture medium B, culturing at 80 ℃ for 16h, replenishing water and ventilating once every morning and afternoon, drying at 80 ℃ for 1d, crushing by a crusher, and sieving by a 40-mesh sieve to obtain the high-temperature bacterial agent, wherein the viable bacteria content of the high-temperature bacteria is 6 multiplied by 10¹¹cfu/g。

The preparation method of the high-temperature bacterium seed solid culture medium A comprises the following steps: 2.5g of yeast extract, 2.5g of bacterial peptone, 2.5g of glucose, 7.5g of PIPES, 10g of plant gel, 10g of agar powder and 1L of sterile water, uniformly mixing, fully dissolving, adjusting the pH value to 7.2-7.4 (adjusting the pH value by solid NaOH), boiling, subpackaging into seeds, sterilizing at 121 ℃ for 30min, and placing on a slope.

The preparation method of the solid medium B comprises the following steps: 100g of rice hull, 100g of bran, 100g of corn flour, 100g of bean cake powder, 100g of glucose, 2.5g of calcium sulfate, 3g of calcium oxide, 1.5g of dipotassium hydrogen phosphate, 1g of magnesium sulfate and 400mL of water, uniformly mixing, and sterilizing at 121 ℃ for 45min to obtain the rice cake.

The bacillus licheniformis is bacillus licheniformis CICC 10037;

the preparation method of the bacillus licheniformis agent comprises the following steps: inoculating slant strain of Bacillus licheniformis to NA seed solid culture medium C, culturing at 40 deg.C for 1d, eluting with sterile water, and adjusting the concentration of the strain to 3 × 10¹⁰cfu/mL, absorbing 100mL of bacterial liquid, inoculating into 1kg of solid culture medium D, culturing at 40 ℃ for 2D, drying at 40 ℃ for 3D, pulverizing with a pulverizer, and sieving with a 40-mesh sieve to obtain the Bacillus licheniformis microbial agent with viable bacteria content of 4 × 10¹⁰cfu/g；

The preparation method of the NA seed solid culture medium C comprises the following steps: uniformly mixing 10g of peptone, 3g of beef powder, 10g of sodium chloride, 20g of agar and 1L of distilled water, fully dissolving, adjusting the pH value to 7.0, boiling, subpackaging in seed bottles, sterilizing at 121 ℃ for 30min for 80-100mL each bottle, and placing on an inclined plane to obtain the beef extract;

the preparation method of the solid medium D comprises the following steps: 260g of rice hull, 600g of bran, 500g of corn flour, 230g of bean cake powder, 230g of starch, 260g of laterite, 55g of glucose, 13g of calcium sulfate, 18g of calcium oxide, 8g of dipotassium hydrogen phosphate, 5g of magnesium sulfate and 1360mL of water, uniformly mixing, and sterilizing at 121 ℃ for 45min to obtain the corn bran-based red mud cake;

the Aspergillus oryzae is Aspergillus oryzae CICC 2001;

the preparation method of the aspergillus oryzae microbial inoculum comprises the following steps: inoculating Aspergillus oryzae slant strain to PDA seed solid culture medium E with inoculating needle, culturing at 28 deg.C for 5d, eluting with sterile water, and adjusting the concentration of thallus to 2 × 10¹¹cfu/mL, absorbing 100mL of bacterial liquid, inoculating into 1kg of solid culture medium B, culturing at 28 deg.C for 5d, drying at 28 deg.C for 3d, pulverizing with a pulverizer, sieving with 40 mesh sieve to obtain Bacillus licheniformis bacterial agent with Aspergillus oryzae viable bacteria content of 8 × 10¹⁰cfu/g。

The preparation method of the PDA seed solid culture medium E comprises the following steps: cutting potato 200g into small pieces, adding appropriate amount of sterile water, decocting for 20min, filtering with gauze to obtain filtrate, glucose 20g, agar 20g, adding distilled water to culture medium volume of 1L, mixing, dissolving completely, boiling, packaging into seed bottles with volume of 80-100mL each, sterilizing at 121 deg.C for 30min, and placing into inclined plane;

the lactobacillus plantarum is lactobacillus plantarum CGMCC 1.2437;

the preparation method of the lactobacillus plantarum microbial inoculum comprises the following steps: inoculating Lactobacillus plantarum slant strain to seed solid culture medium C with inoculating needle, culturing at 35 deg.C for 2 days, eluting thallus with sterile water, and adjusting thallus concentration to 4 × 10¹⁰cfu/mL, absorbing 100mL of bacterial liquid, inoculating into 1kg of solid culture medium F, culturing at 35 ℃ for 2d, drying at 35 ℃ for 2d, pulverizing with a pulverizer, and sieving with a 40-mesh sieve to obtain Lactobacillus plantarum microbial inoculum with viable bacteria content of 8 × 10¹⁰cfu/g。

The preparation method of the solid culture medium F comprises the following steps: 400g of bean cake powder, 350g of rice hull, 120g of cottonseed meal, 3g of peptone, 2g of glucose, 0.2g of magnesium sulfate and 547mL of water, uniformly mixing, and sterilizing at 121 ℃ for 45min to obtain the soybean cake.

The Hill-bacterium Saccharum is the Hill-bacterium Saccharum CGMCC No. 13928;

the preparation method of the Lanchow bacterium agent comprises the following steps: inoculating slant strain of Hill-Saccharum into NA seed solid culture medium C with inoculating needle, culturing at 60 deg.C for 1d, eluting with sterile water, scraping off thallus, and adjusting thallus concentration to 5 × 10¹¹cfu/mL, absorbing 100mL of bacterial liquid, inoculating the bacterial liquid into 1kg of solid culture medium A, culturing for 4 days at 60 ℃, drying for 2 days at 60 ℃, crushing by using a crusher, and sieving by using a 40-mesh sieve to obtain the Hizikia saccharalis CP microbial inoculum, wherein the content of viable bacteria of the Hizikia saccharalis is 7 multiplied by 10¹¹cfu/g；

The preparation method of the cold-resistant brevibacterium inoculant comprises the following steps: inoculating the slant strain of Brevibacterium frigidum to NA seed solid culture medium C with inoculating needle, culturing at 10 deg.C for 3d, eluting with sterile water, scraping, and adjusting the concentration to 8 × 10¹⁰cfu/mL, absorbing 100mL of bacterial liquid, inoculating the bacterial liquid into 1kg of solid culture medium G, culturing for 3d at 10 ℃, drying for 7d at 10 ℃, crushing by using a crusher, and sieving by using a 40-mesh sieve to obtain the sugarcane orchid bacterium agent, wherein the viable bacteria content of the brevibacterium frigidum is 3 multiplied by 10¹¹cfu/g；

The preparation method of the solid medium G comprises the following steps: 600g of bran, 200g of rice hull, 100g of peanut cake powder, 100g of soybean meal, 0.5g of magnesium sulfate, 5g of ammonium sulfate and 1L of water, uniformly mixing, and sterilizing at 121 ℃ for 45min to obtain the peanut cake.

Example 2 preparation method of straw and cow dung ultrahigh-temperature decomposing inoculant

(1) And (3) mixing the microbial inoculum: mixing 3 parts of a thermophilic bacteria agent, 2 parts of a bacillus licheniformis agent, 2 parts of an aspergillus oryzae agent, 2 parts of a lactobacillus plantarum agent, 2 parts of a lancina sacchari agent and 2 parts of a cold-resistant brevibacterium agent, and sieving by a 30-mesh sieve to obtain a thoroughly decomposed composite microbial agent;

(2) mixing fresh straws and cow dung according to the mass ratio of 11:6 to obtain a mixed pile body, wherein the water content of the mixed pile body is 60-65%;

(3) inoculating the decomposed complex microbial inoculum into a mixed compost body according to the inoculation amount of 1.5 per mill in mass ratio, mixing and stirring, carrying out decomposition treatment under the condition that the initial composting temperature is 10 ℃, and composting for 10d to obtain the ultrahigh-temperature decomposing agent for straw and cow dung.

The preparation method of the thermophilic bacteria agent comprises the following steps: inoculating thermophilic bacteria slant strain to high-temperature bacteria seed solid culture medium A, culturing at 75 deg.C for 4 days, eluting thallus with sterile water, and adjusting thallus concentration to 5 × 10¹¹cfu/mL, absorbing 125mL of bacterial liquid, inoculating into 1kg of solid culture medium B, culturing at 75 ℃ for 5d, replenishing water and ventilating once every morning and afternoon, drying at 75 ℃ for 1d, crushing by a crusher, and sieving by a 40-mesh sieve to obtain the high-temperature bacterial agent, wherein the viable bacteria content of the high-temperature bacteria is 6.5 multiplied by 10¹¹cfu/g。

The bacillus licheniformis is bacillus licheniformis CICC 10084;

the Aspergillus oryzae is Aspergillus oryzae CICC 2011;

the lactobacillus plantarum is lactobacillus plantarum CGMCC 1.2437;

the Hill-bacterium Saccharum is the Hill-bacterium Saccharum CGMCC No. 13928;

the preparation methods and the bacterial activity contents of the bacillus licheniformis microbial agent, the aspergillus oryzae microbial agent, the lactobacillus plantarum microbial agent and the lancina saccharatum microbial agent are the same as those in the embodiment 1;

in the preparation process, the preparation methods of the seed solid culture medium A, the solid culture medium B, the seed solid culture medium C, the solid culture medium D, the seed solid culture medium E, the solid culture medium F and the solid culture medium G are the same as the example 1.

Example 3 preparation method of straw and cow dung ultrahigh-temperature decomposing inoculant

(1) And (3) mixing the microbial inoculum: mixing 8 parts of a thermophilic bacteria agent, 5 parts of a bacillus licheniformis agent, 5 parts of an aspergillus oryzae agent, 5 parts of a lactobacillus plantarum agent, 5 parts of a lancina sacchari agent and 5 parts of a cold-resistant brevibacterium agent, and sieving by a 40-mesh sieve to obtain a thoroughly decomposed composite microbial agent;

(2) mixing fresh straws and cow dung according to the mass ratio of 12:5 to obtain a mixed pile body, wherein the water content of the mixed pile body is 55-65%;

(3) inoculating the decomposed complex microbial inoculum into a mixed compost body according to the inoculation amount of 1-2 per mill by mass ratio, mixing and stirring, carrying out decomposition treatment under the condition that the initial composting temperature is 11 ℃, and composting for 12d to obtain the ultrahigh-temperature decomposing agent for straw and cow dung.

The preparation method of the thermophilic bacteria agent comprises the following steps: inoculating thermophilic bacteria slant strain to high-temperature bacteria seed solid culture medium A, culturing at 82 deg.C for 19 hr, eluting thallus with sterile water, and adjusting thallus concentration to 6 × 10¹¹cfu/mL, absorbing 100mL of bacterial liquid, inoculating into 1.2kg of solid culture medium B, culturing at 82 ℃ for 19h, replenishing water and ventilating once in the morning and afternoon of each day, drying at 82 ℃ for 1d, crushing by a crusher, and sieving by a 40-mesh sieve to obtain the high-temperature bacterial agent, wherein the content of viable bacteria of the high-temperature bacteria is 7 multiplied by 10¹¹cfu/g。

The preparation method of the cold-resistant brevibacterium inoculant comprises the following steps: inoculating the slant strain of Brevibacterium frigidum to NA seed solid culture medium C with inoculating needle, culturing at 10 deg.C for 3d, eluting with sterile water, scraping, and adjusting the concentration to 9 × 10¹⁰cfu/mL, absorbing 100mL of bacterial liquid, inoculating the bacterial liquid into 1kg of solid culture medium G, culturing for 3d at 10 ℃, drying for 7d at 10 ℃, crushing by using a crusher, and sieving by using a 40-mesh sieve to obtain the sugarcane orchid bacterium agent, wherein the viable bacteria content of the brevibacterium frigosum is 4.5 multiplied by 10¹¹cfu/g；

The bacillus licheniformis is bacillus licheniformis CICC 10085;

the Aspergillus oryzae is Aspergillus oryzae CICC 2016;

the lactobacillus plantarum is lactobacillus plantarum CGMCC 1.2437;

the Hill-bacterium Saccharum is the Hill-bacterium Saccharum CGMCC No. 13928;

Example 4 preparation method of straw and cow dung ultrahigh-temperature decomposing inoculant

(1) And (3) mixing the microbial inoculum: mixing 1 part of a thermophilic bacteria agent, 1 part of a bacillus licheniformis agent, 1 part of an aspergillus oryzae agent, 1 part of a lactobacillus plantarum agent, 1 part of a lancina saccharatum agent and 1 part of a cold-resistant brevibacterium agent, and sieving by a 40-mesh sieve to obtain a thoroughly decomposed composite agent;

(2) mixing fresh straws and cow dung according to the mass ratio of 6:1 to obtain a mixed pile body, wherein the water content of the mixed pile body is 55-65%;

(3) inoculating the decomposed complex microbial inoculum into a mixed compost body according to the inoculation amount of 2 per mill of the mass ratio, mixing and stirring, carrying out decomposition treatment under the condition that the initial composting temperature is 12 ℃, and composting for 10 days to obtain the ultrahigh-temperature decomposing agent for straw and cow dung.

The preparation method of the thermophilic bacteria agent comprises the following steps: inoculating thermophilic bacteria slant strain to high-temperature bacteria seed solid culture medium A, culturing at 78 deg.C for 18 hr, eluting thallus with sterile water, and adjusting thallus concentration to 7 × 10¹¹cfu/mL, absorbing 100mL of bacterial liquid, inoculating into 1.0kg of solid culture medium B, culturing at 78 ℃ for 16h, replenishing water and ventilating once every morning and afternoon, drying at 78 ℃ for 1d, pulverizing with a pulverizer, and sieving with a 40-mesh sieve to obtain the high-temperature bacterial agent, wherein the content of viable bacteria of the high-temperature bacteria is 7.2 × 10¹¹cfu/g。

Preparation method and package of cold-resistant brevibacterium inoculantThe method comprises the following steps: inoculating the slant strain of Brevibacterium frigidum to NA seed solid culture medium C with inoculating needle, culturing at 10 deg.C for 3d, eluting with sterile water, scraping, and adjusting the concentration to 8 × 10¹⁰cfu/mL, absorbing 100mL of bacterial liquid, inoculating the bacterial liquid into 1kg of solid culture medium G, culturing for 3d at 10 ℃, drying for 7d at 10 ℃, crushing by using a crusher, and sieving by using a 40-mesh sieve to obtain the sugarcane orchid bacterium agent, wherein the viable bacteria content of the brevibacterium frigidum is 3 multiplied by 10¹¹cfu/g；

The bacillus licheniformis is bacillus licheniformis CICC 10092;

the Aspergillus oryzae is Aspergillus oryzae CICC 2022;

the lactobacillus plantarum is lactobacillus plantarum CGMCC 1.2437;

the Hill-bacterium Saccharum is the Hill-bacterium Saccharum CGMCC No. 13928;

Example 5 preparation method of straw and cow dung ultrahigh-temperature decomposing inoculant

(2) mixing fresh straws and cow dung according to the mass ratio of 18: 5, mixing to obtain a mixed pile body, wherein the water content of the mixed pile body is 55-65%;

The preparation method of the thermophilic bacteria agent and the packageThe method comprises the following steps: inoculating thermophilic bacteria slant strain to high-temperature bacteria seed solid culture medium A, culturing at 80 deg.C for 18 hr, eluting thallus with sterile water, and adjusting thallus concentration to 5 × 10¹¹cfu/mL, absorbing 100mL of bacterial liquid, inoculating into 1.2kg of solid culture medium B, culturing at 80 ℃ for 17h, replenishing water and ventilating once every morning and afternoon, drying at 80 ℃ for 1d, pulverizing with a pulverizer, and sieving with a 40-mesh sieve to obtain the high-temperature bacterial agent, wherein the viable bacteria content of the high-temperature bacteria is 5.6 multiplied by 10¹¹cfu/g。

The bacillus licheniformis is bacillus licheniformis CICC10037 and bacillus licheniformis CICC 10084;

the Aspergillus oryzae is Aspergillus oryzae CICC2001, Aspergillus oryzae CICC2011 and Aspergillus oryzae CICC 2016;

the lactobacillus plantarum is lactobacillus plantarum CGMCC 1.2437;

the Hill-bacterium Saccharum is the Hill-bacterium Saccharum CGMCC No. 13928;

in the preparation process, the preparation methods of the seed solid culture medium A, the solid culture medium B, the seed solid culture medium C, the solid culture medium D, the seed solid culture medium E, the solid culture medium F and the solid culture medium G are the same as the example 1;

the bacillus licheniformis CICC10037 and the bacillus licheniformis CICC10084 microbial inoculum are uniformly mixed according to the mass ratio of 2: 1;

the Aspergillus oryzae CICC2001, Aspergillus oryzae CICC2011 and Aspergillus oryzae CICC2016 bacterial agents are uniformly mixed according to the mass ratio of 1:1: 1.

Example 6 thermophilic bacteria pH tolerance test

Selecting a proper amount of thalli from thermophilic bacteria slant strains, inoculating the thalli into 100mL of high-temperature bacteria liquid culture medium, carrying out shake culture at 80 ℃ and 200r/min for 12h to obtain seed liquid, inoculating the seed liquid into the high-temperature bacteria liquid culture medium with the pH of 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 10.5, 11, 11.5 and 12 respectively in an inoculation amount of 1 per thousand (volume ratio), carrying out shake culture at 80 ℃ and 200r/min for 16h, coating an NA plate, and observing the growth condition; and simultaneously inoculating the seed solution into a high-temperature bacteria liquid culture medium test tube with the same pH value gradient by 1 per mill of inoculation amount, carrying out shake culture at 80 ℃ for 16h at 200r/min, and observing turbidity.

Wherein the high-temperature bacterium liquid culture medium comprises the following components: yeast extract 2.5g, peptone 2.5g, glucose 2.5g, PIPES 7.5g, pH 7.2-7.4 (adjusted by solid NaOH), and water 1L

And (3) test results: thermophilic bacteria are cultured in high temperature bacteria liquid culture medium with pH value of 3-11 at 80 deg.C for 16h, and the coated NA plate can grow well, and the bacteria activity content at pH value of 3 is 1-2 × 10¹⁰cfu/ml, pH 11 bacteria activity content of 2-4 × 10¹⁰cfu/ml, pH 5-9, and activity content of 5-6 × 10¹¹cfu/ml; after being cultured in a shake bed for 16h at the temperature of 80 ℃ and 200r/min in an NA liquid culture medium test tube, the growth is good, the turbidity is obvious, and the activity content of the bacteria detected by the pH value of 5-9 is 4.5-5.5 multiplied by 10¹¹cfu/ml, and both tests prove that the thermophilic bacteria can tolerate pH within the range of 3-11 and have wide tolerance of pH value.

Example 7 thermophilic bacteria temperature tolerance and genetic stability experiments

A proper amount of mycelia was selected from thermophilic bacteria slant culture, inoculated into 100mL of high temperature bacteria liquid culture medium (the composition was the same as in example 6), shake-cultured at 80 ℃ and 200r/min for 12 hours to obtain a seed solution, which was spread on a plate of a solid culture medium H (wherein the culture medium used for the plate was the high temperature bacteria seed solid slant culture medium A), and cultured at 40 ℃, 45 ℃, 50 ℃, 55 ℃, 60 ℃, 65 ℃, 70 ℃, 75 ℃, 80 ℃, 85 ℃, 90 ℃, 95 ℃, 100 ℃, 105 ℃, 110 ℃ for 18 hours, respectively, to observe the growth. Meanwhile, inoculating the seed solution into a high-temperature bacterium liquid culture medium test tube in an inoculation amount of 1 per mill (volume ratio), respectively placing the test tubes under the same temperature gradient, carrying out shaking table culture at 200r/min for 16h, and observing turbidity.

Wherein, the solid culture medium H comprises the following components: 2.5g of yeast extract, 2.5g of peptone, 2.5g of glucose, 7.5g of PIPES, 7.2-7.4 of pH value (regulated by solid NaOH), 1% of plant gel, 1% of agar and 1L of water.

And (3) test results: the thermophilic bacteria can grow well at 50-100 deg.C on a coated plate after 12 hr liquid culture, and the viable bacteria content is 5.5-6.0 × 10 by detecting culture at 100 deg.C¹¹cfu/ml; after being cultured in a high-temperature bacterium liquid culture medium test tube at 200r/min for 16h in a shaking table, the growth is good, the turbidity is very obvious, and the activity content of the detected bacterium is 5.5-6.5 multiplied by 10¹¹cfu/ml, and both tests prove that the thermophilic bacteria can tolerate the growth temperature of 50-100 ℃ and have higher temperature tolerance.

The thermophilic bacteria are subcultured according to the method, and the bacterial activity of each generation is detected to be not less than 10 after ten times of subculture¹⁰cfu/mL, and the growth morphology and characteristics are not changed, which shows that the strain can be stably inherited and can grow well.

Example 8 acid and alkali resistance measurement of Brevibacterium frigidum

The experimental method comprises the following steps: (1) adjusting the pH value of each culture medium bottle by using a pH meter to 3, 4, 5, 6, 7, 8, 9, 10, 11 and 12 respectively, sterilizing and pouring into a flat plate.

(2) Inoculating the strain of brevibacterium frigosum slant to NA seed solid culture medium by using an inoculating needle, culturing for 4d at 10 ℃, eluting and scraping the strain by using sterile water, diluting and coating the strain on 10 culture media with different pH values, and culturing for 1-2 days at 28 ℃ to observe the growth condition of the strain.

Wherein, the NA culture medium comprises: 10g of peptone, 3g of beef powder, 5g of NaCl, 7.2 of PH, 1% agar and 1L of water.

The experimental results are as follows: through observation of the growth conditions of bacterial colonies on plates with different pH values, the brevibacterium frigosum grows well on culture media with pH values of 5, 6, 7, 8, 9, 10, 11 and 12, and the strain activity is 3-5 multiplied by 10¹⁰One per ml. The solid culture medium with pH value of 3, 4 is not plated on the plateSolidifying, and preparing liquid culture medium with pH of 3 and 4 to shake culture strain with activity of 2-5 × 10¹⁰One per ml. From experimental results, the pH value of the Brevibacterium fritolerant strain is at least 3-12, and is possibly wider, and the strain activity of the pH value in the range of 3-12 is 0.5-2 × 10¹⁰One per ml.

Example 9 salt tolerance assay of Brevibacterium fritolerans

The experimental method comprises the following steps: (1) adding NaCl into each conical flask according to the proportion of 2%, 5%, 10% and 15%, sterilizing and pouring into a flat plate.

(2) Inoculating the strain of brevibacterium frigosum slant to NA seed solid culture medium by using an inoculating needle, culturing for 4d at 10 ℃, eluting and scraping the strain by using sterile water, diluting and coating the strain on four culture media with different salinity, activating 4 plates on each culture medium by each strain, and culturing for 1-2 days at 28 ℃ to observe the growth condition of the strain.

The experimental results are as follows: the hardy brevibacterium grows well on culture medium with salinity of 2%, 5%, 10% and 15%, and the strain activity reaches 0.5-4 × 10¹⁰One per ml.

Example 10 detection of temperature Range of tolerance to Brevibacterium fritolerans

The experimental method comprises the following steps: inoculating brevibacterium frigosum slant strain to NA seed solid culture medium with inoculating needle, culturing at 10 deg.C for 3d, eluting thallus with sterile water, scraping, diluting, spreading onto NA plate, culturing at 4 deg.C, 7 deg.C, 25 deg.C, 30 deg.C, 35 deg.C, 40 deg.C, 50 deg.C, 60 deg.C, and 70 deg.C for 1-2 days, and observing the result, with 4 plates per strain at each temperature as parallel.

The experimental results are as follows:

TABLE 1 Strain Activity at different temperatures

"+ + + +" indicates vigorous growth and 2-4X 10 of strain viability¹⁰One per ml, "+ +" indicates good growth and 0.5-2X 10 of strain viability¹⁰One per ml.

From the experimental results, the Brevibacterium fritolerant strain can grow well when cultured at 4 ℃, 7 ℃, 25 ℃, 30 ℃, 35 ℃, 40 ℃ and 50 ℃, but does not grow when cultured at 60 ℃ and 70 ℃. Therefore, it can be determined that the temperature range for the growth of Brevibacterium hardtii is at least 4 ℃ to 50 ℃, and the temperature range is possibly wider.

Example 11 detection of proteolytic Activity of Brevibacterium fritolerans

2.1.3 culture Medium

The experimental method comprises the following steps: preparing a protein decomposing culture medium, sterilizing, pouring the flat plate, inoculating the brevibacterium frigosum to the protein decomposing flat plate after the culture medium is solidified, inoculating 6 flat plates, culturing at 28 ℃ for 3 days, and observing an experimental result. Wherein: protein degradation culture medium: 3g of beef extract powder, 5g of sodium chloride, 2g of casein, 20g of agar powder and 1L of distilled water. Dissolving casein with small amount of 2% sodium hydroxide, wetting, stirring, adding appropriate amount of distilled water, boiling for dissolving, fixing volume, pH 7.6, and sterilizing at 121 deg.C for 30 min.

The experimental results are as follows: through observing the experimental result, the following results are found: the protein decomposing plate is provided with a protein decomposing ring, which shows that the brevibacterium frigosum has remarkable protein decomposing property, and the diameter of the protein ring is 3.2 cm.

Example 12 detection of starch-degrading Properties of Cold-resistant Brevibacterium

The experimental method comprises the following steps: and (3) sterilizing a starch-dissolving culture medium, pouring the culture medium into a flat plate, inoculating the brevibacterium frigosum to the starch-dissolving flat plate after the culture medium is solidified, inoculating 6 flat plates, culturing for 5 days at 28 ℃, dyeing the flat plates by using iodine solution, and observing an experimental result. Wherein: the composition of the starch decomposition culture medium is as follows: 10g of soluble starch, 10g of peptone, 3g of beef powder, 5g of sodium chloride, 1L of distilled water, 20g of agar and 7.2 of pH value.

The experimental results are as follows: through observing the experimental result, the following results are found: the starch-degrading flat plate is provided with a starch-degrading ring, and the diameter of the starch ring is 1.4cm, which indicates that the cold-resistant brevibacterium has the characteristic of degrading starch.

Example 13 fat-resolving ability of Brevibacterium fritolerans was examined.

The experimental method comprises the following steps: preparing a fat-dissolving culture medium, sterilizing, pouring a flat plate, inoculating the hardy brevibacterium to the fat-dissolving flat plate after the culture medium is solidified, and inoculating 6 flat plates for 5 days at 28 ℃ due to the fear of serious pollution of the flat plate, and observing the experimental result. Wherein the fat-decomposing culture medium: 10g of peptone, 5g of sodium chloride, 0.1g of calcium chloride, 7.4 of pH value, 1L of distilled water and 20g of agar powder, sterilizing at 121 ℃ for 30min, cooling to 40-50 ℃, respectively adding Tween40, Tween60 and Tween80 which are sterilized separately until the final concentration is 1%, shaking uniformly and pouring the mixture into a flat plate.

The experimental results are as follows: through observing the experimental result, the following results are found: the fat-decomposing plate is provided with a fat-decomposing ring, the diameter of the fat ring is 3cm, and the result shows that the hardy brevibacterium has remarkable fat-decomposing property.

Example 13 straw and cow dung compost maturity experiment by using ultra-high temperature maturity microbial inoculum

1. Straw and cow dung composting and decomposing process

The straw and cow dung decomposing inoculant prepared in the embodiment 1 of the invention is inoculated into a heap with pH of 5.7 and water content of 60% by an inoculation amount of 1.5 per mill by mass ratio, and is uniformly mixed, wherein the heap top width is 1 meter, the heap bottom width is 1.8 meters, the heap height is 1-1.2 meters, and the heap length is 200 meters. The initial temperature of the compost is 10 ℃, when the temperature is higher than 60 ℃, the compost is turned by a turning vehicle once, and then the compost is turned once every 1 to 2 days. The temperature was measured from five different locations of the compost each time, and a time point was selected for measurement each day, and the compost was turned over every 3-5 days (this treatment was test group L).

Two compost decomposing treatments with the same raw materials and scale sizes are set as controls and are respectively defined as CK1 and CK2, CK1 is inoculated with a commercially available decomposing inoculant, CK2 is not inoculated with the decomposing inoculant, and the test operation steps and the method are the same as those of the experimental group L.

Wherein the piles of the experimental group L and the control groups CK1 and CK2 are all fresh straws and cow dung according to the mass ratio of 16: 5 to obtain a mixed pile body, wherein the water content of the mixed pile body is 60 percent.

2. Test result of comparison between experimental group L and control group CK1 and CK2

2.1 temperature

As can be seen from the attached figure 1, under the condition that the initial composting temperature is 10 ℃, the temperature rise speed of the compost of an experimental group L is fastest, the 1 st day of the compost can reach 60 ℃, the 3 rd day of the compost reaches the highest temperature of 98 ℃ and lasts for 4 days at 88-95 ℃, the harmless standard of the compost can be reached, the composting is completed in 15 days of fermentation, and the whole process of the composting is completed in more than 70 ℃ for 7-10 days; the 5 th day of the CK1 compost of the control group reaches 60 ℃, the 8 th day of the CK1 compost reaches the maximum temperature of 75 ℃ and is only maintained for 1 day, the maximum temperature reached by the experimental group L is 23 ℃ higher than that of the CK1 of the control group, the CK1 of the control group finishes decomposing after 43 days, and the time for finishing decomposing by the experimental group L is 28 days earlier than that of the CK1 of the control group; the control CK2 reached the maximum temperature of 58 ℃ on day 12, and the decomposition was completed in 55 days, and the time for completion of the decomposition of the experimental group L was 40 days earlier than that of the control CK 2. The results show that the inoculation decomposition agent can accelerate the degradation of organic matters in the straw and cow dung compost, and compared with the commercially available straw and cow dung decomposition agent, the straw and cow dung ultrahigh-temperature decomposition agent prepared by the method has the remarkable advantages of low compost decomposition starting temperature, strong environment adaptability, high fermentation efficiency, high temperature rising speed, high maximum temperature, long high-temperature maintaining period, short compost decomposition fermentation period and the like.

2.2pH value

As can be seen from the attached FIG. 2, the pH values of the control CK1, the control CK2 and the test group L at the end of composting fermentation are 7.6, 7.8 and 7.3 respectively, and in general, the pH value of the inoculated decomposing agent compost is increased, but the lower pH value of the test group compared with the control group can reduce the volatilization of ammonia in the compost, thereby reducing the loss of nitrogen.

2.3 organic content

As can be seen from fig. 3, the organic content of the control CK1, the control CK2 and the experimental group L at the end of composting is 57.46%, 62.38% and 46.27%, respectively, which are respectively reduced by 33.97%, 27.05% and 43.16% compared with the initial organic content of 86.43%; according to the change of the organic matter content, the degradation effect of the inoculated decomposing inoculant on the organic matters of the compost is larger, and the degradation efficiency of the straw and cow dung ultra-temperature decomposing inoculant prepared by inoculating the method is obviously higher than that of the commercially available straw and cow dung decomposing inoculant.

2.4 Total Nitrogen content

As can be seen from the attached FIG. 4, when the compost is completely decomposed, the total nitrogen content of the experimental group L is 2.78%, the total nitrogen content of the control group CK1 is 1.62%, and the total nitrogen content of the control group CK2 is 1.28%. Compared with the three treatments, the total nitrogen content of the inoculated decomposing inoculant in the stack is obviously higher than that of a control group CK1, and the total nitrogen content of the inoculated straw and cow dung ultrahigh-temperature decomposing inoculant prepared by the method is slightly higher than that of the commercially available straw and cow dung high-temperature decomposing inoculant.

2.5 Water content

As can be seen from the attached figure 5, when the compost is completely decomposed, the water content of the experimental group L is 20.5%, the water content of the control group CK1 is 26.8%, the water content of the control group CK2 is 28.9%, and the water content of the inoculated ultrahigh-temperature decomposing inoculant provided by the invention is obviously lower than that of the inoculated commercial decomposing inoculant. Therefore, the addition of the decomposition microbial inoculum of the invention can lead the highest fermentation temperature to reach 98 ℃, which is the highest temperature which is not found so far, and the time for entering the high temperature is shorter, and the time for maintaining the highest temperature is longer, thus leading the water to be volatilized more quickly.

As can be seen from Table 2, the nitrogen, phosphorus and potassium contents (%) of each treatment were determined after the completion of the decomposition.

TABLE 2 Nitrogen, phosphorus and Potassium contents (%)

Note: P1-P5 are five parallel test groups

As can be seen from Table 2, the total amount of N, P, K specified in the organic fertilizer industry standard NY525-2012 should not be less than 5%, the organic matter content should not be less than 45%, and the pH value should be between 5.5-8.5, while the leavening agent of the present invention meets the industry standard. It should be noted that the straw and cow dung ultrahigh-temperature decomposition agent prepared in the embodiments 2-5 of the present invention also have the above-mentioned experimental effects, and the difference between the embodiments is not significant.

Example 14 detection of quality index of straw and cow dung ultra-high temperature decomposition agent

The straw and cow dung ultrahigh-temperature decomposition agent prepared in the embodiment 1 of the invention is used as a sample, and the technical indexes and harmless indexes of the organic fertilizer are detected according to national standard detection methods such as GB/T19524.1-determination of faecal coliform group in 2004 fertilizer, GB/T19524.2-determination of ascarid egg death rate in 2004 fertilizer, NY/T1978-determination of mercury, arsenic, lead and chromium contents in 2010 fertilizer, and the results are shown in tables 3 and 4:

table 3: technical indexes of biological organic fertilizer

Item	Standard value NY884-2012	Measured value
			Effective viable count (cfu/g)	≥0.2	6×10¹¹
Organic matter (dry basis,%)	≥40	46.27
			Moisture (%)	≤30	20.5
pH	5.5-8.5	7.3
			Effective period (moon)	≥6	12

Table 4: harmless index of bio-organic fertilizer

Item	Standard value NY884-2012	Measured value
			Faecal coliform count (number/g)	≤100	50
Death rate (%) of roundworm egg	≥95	99
			Total arsenic (As) (mg/kg on a dry basis)	≤15	9
Total cadmium (Cd) (mg/kg on a dry basis)	≤3	2
			Total lead (Pb) (mg/kg on a dry basis)	≤50	39
Total chromium (Cr) (mg/kg on a dry basis)	≤150	99
			Total mercury (Hg) (mg/kg on a dry basis)	≤2	0.8

The detection results show that the technical indexes and harmless indexes of the straw and cow dung ultrahigh-temperature decomposition agent prepared by the method reach or are superior to those of the quality calibration of the bio-organic fertilizer of Ministry of agriculture, a new shortcut is opened for the rapid and efficient high-temperature composting fermentation of the straw and cow dung to prepare the high-quality bio-organic fertilizer, and the method has better advancement and practicability.

It should be noted that the straw and cow dung ultrahigh-temperature decomposition agent prepared in the embodiments 2-5 of the present invention also have the above-mentioned experimental effects, and the difference between the embodiments is not significant.

Example 15 application effect test of straw and cow dung organic fertilizer in rape cultivation

1 test materials:

1.1 test plants: rape seed

1.2 test fertilizers: straw and cow dung ultrahigh-temperature organic fertilizer, commercial organic fertilizer and chemical fertilizer prepared in embodiment 1 of the invention

2, test method:

2.1 test treatment: the experiment is divided into four groups, namely T1 is not fertilized; t2 is a fertilizer; t3 is a commercial organic fertilizer; t4 is the straw and cow dung ultra-high temperature decomposition agent prepared in the embodiment 1 of the invention

2.2 test procedure: respectively adding four groups of fertilizers of T1, T2, T3 and T4 into potted soil according to the mass percentage of 0.05-0.15% of the fertilizers and the soil, wherein each potted soil contains 8kg of soil, and (the compositions of the four groups of soil of T1, T2, T3 and T4 are completely the same) selecting rapeseeds with full seeds, respectively sowing and field planting, sowing 10 seeds in each pot, and treating three repeated parallel experimental groups by each group of fertilizers. 50 days after rape sowing, indexes such as the yield, the plant height, the total nitrogen content, the soil fertility and the like of each treatment are measured, and the detection results are shown in tables 5 to 6.

3 results and analysis:

TABLE 5 yield and quality of rape at harvest stage for each treatment

TABLE 6 soil fertility at harvest stage of rape processed

As can be seen from Table 5, the T4 ultrahigh-temperature straw and cow dung organic fertilizer treatment method has the best growth and best quality of the rape, and the yield, the plant height and the chlorophyll content of the rape are higher than those of the rape treated by chemical fertilizers and commercially available organic fertilizers. The compost products fermented by the straw and cow dung ultrahigh-temperature decomposing inoculant obviously improve the soil fertility, are beneficial to the growth of rape and improve the yield of the rape.

As can be seen from Table 6, the contents of organic matters, hydrolyzed nitrogen, available phosphorus and quick-acting potassium in the rape harvest period treated by T4 are respectively increased by 7.46%, 31.1%, 36.3% and 42% compared with the rape harvest period treated without fertilization; compared with the fertilizer treatment, the amount of the fertilizer is increased by 6.64 percent, 27.7 percent, 7.7 percent and 53 percent; compared with the treatment of commercial organic fertilizer, the treatment amount is increased by 4.71%, 15.4%, 12.6% and 21%.

The rape yield was individually picked and recorded in groups and the data was analysed using the Duncan's new repolarisation test method of the DPS software (see table 7, table 8):

table 7: analysis of variance table

Cause of change	Sum of squares	Degree of freedom	Mean square	F value	Significance of
						Treatment room	1.4811	3	0.4937	1519.077	Is extremely remarkable
In-process	0.0026	8	0.0003
						Total variation	1.4837	11

Table 8: new repolarization differential method testing table for yield result

Note that lower case letters indicate the significance of the difference at the 5% level and upper case letters indicate the significance of the difference at the 1% level.

From table 7, it can be seen that F value (3, 8) ═ 1519.077> F0.01(3, 8) ═ 7.59, i.e. the difference between different treatments is very significant, and the application of straw and cow dung organic fertilizer has obvious effect of improving the yield of rape. At the level of difference between 1% and 5% (table 8), the yield of rape treated by 4 (applying straw and cow dung ultra-high temperature decomposed organic fertilizer) and 2 (applying fertilizer) is obviously improved greatly compared with that of treatment 3 using commercial organic fertilizer and treatment 1 without fertilizer. The yield of the rapes treated by the treatment 1, the treatment 2, the treatment 3 and the treatment 4 is very different at a 5% level and a 1% level, wherein the yield of the organic fertilizer decomposed at ultrahigh temperature by applying straw and cow dung is the highest.

The chlorophyll content of the rape is picked up and recorded separately in groups, and the data is analyzed by Duncan's new double-pole difference test method of DPS software (see tables 9 and 10):

table 9: analysis of variance table

Cause of change	Sum of squares	Degree of freedom	Mean square	F value	Significance of
						Treatment room	212.9624	3	70.9875	283.95	Is extremely remarkable
In-process	2	8	0.25
						Total variation	214.9624	11

Table 10: new repolarization differential method test table for chlorophyll content result

Treatment of	Mean value	5% significant level	1% very significant level
				Treatment
4	35.6	a	A
				Treatment
3	33.8	b	B
				Treatment
2	31.2	c	C
				Process
1	24.5	d	D

From table 9, it can be seen that F value (3, 8) ═ 283.95> F0.01(3, 8) ═ 7.59, i.e. the difference between different treatments is very significant, and the application of straw and cow dung ultra-high temperature organic fertilizer has obvious effect of improving the chlorophyll content of rape. At the level of difference between 1% and 5% (table 10), the chlorophyll content of the rape seeds treated by the treatment 4 (applying straw and cow dung ultra-high temperature decomposed organic fertilizer) and the treatment 3 (applying commercial organic fertilizer) is obviously improved greatly compared with the treatment 2 using chemical fertilizer and the treatment 1 without applying fertilizer. The chlorophyll content of the rape seeds treated by the treatment 1, the treatment 2, the treatment 3 and the treatment 4 is remarkably different from that of the rape seeds treated by the treatment 1 at the level of 5% and that of the rape seeds treated by the treatment 4 at the level of 1%, wherein the chlorophyll content of the organic fertilizer decomposed at the ultrahigh temperature by applying the straws and the cow dung is the highest.

The rape plant height is picked up and recorded separately in groups, and the data is analyzed by Duncan's new repolarization test method of DPS software (see tables 11 and 12):

table 11: analysis of variance table

Cause of change	Sum of squares	Degree of freedom	Mean square	F value	Significance of
						Treatment room	4130.25	3	1376.75	1694.462	Is extremely remarkable
In-process	6.5	8	0.8125
						Total variation	4136.75	11

Table 12: new repolarization differential method test table for plant height and fruit yield

Treatment of	Mean value	5% significant level	1% very significant level
				Treatment
4	82	a	A
				Treatment
2	78	b	B
				Treatment
3	60	c	C
				Process
1	35	d	D

From table 11, it can be seen that F value (3, 8) ═ 1694.462> F0.01(3, 8) ═ 7.59, i.e. the difference between different treatments is very significant, and the application of straw and cow dung organic fertilizer has obvious effect of improving the plant height of rape. At the level of difference between 1% and 5% (table 12), the heights of the rapes treated by 4 (applying straw and cow dung ultra-high temperature decomposed organic fertilizer) and 2 (applying fertilizer) are obviously improved greatly compared with the height of the rapes treated by 3 and 1 without fertilizer. The yield of the rapes treated by the treatment 1, the treatment 2, the treatment 3 and the treatment 4 is very different at a 5% level and a 1% level, wherein the height of the rapes subjected to the ultrahigh-temperature decomposed organic fertilizer by applying straws and cow dung is the highest.

The rape accumulated nitrogen uptake is picked up and recorded separately in groups, and the data is analyzed by Duncan's new repolarization test method of DPS software (see tables 13 and 14):

table 13: analysis of variance table

Table 14: new repolarization difference method testing table for accumulated nitrogen uptake result of rape

Treatment of	Mean value	5% significant level	1% very significant level
				Treatment
3	16.32	a	A
				Treatment
4	10.86	b	B
				Treatment
2	8.95	b	B
				Process
1	3.58	c	C

From table 13, it is known that F value (3, 8) ═ 82.98> F0.01(3, 8) ═ 7.59, i.e. the difference between different treatments is very significant, and the cumulative nitrogen uptake of rapes applied with commercial organic fertilizer has a significant improvement effect. On the level of difference between 1% and 5% (table 14), the accumulated nitrogen absorption of rapes treated by 4 (applied with straw and cow dung ultra-high temperature decomposed organic fertilizer) and 2 (applied with fertilizer) is obviously improved compared with that of treatment 1 without fertilizer. The cumulative nitrogen absorption of the rapes treated by the treatment 4 (application of straw and cow dung ultra-high temperature decomposed organic fertilizer) and the treatment 2 (application of fertilizer) is not obviously different at the level of 5% and at the level of 1%.

Randomly borrowing soil to detect the content of organic matters in the soil, and analyzing the data by using a Duncan's new repolarization test method of DPS software (see tables 15 and 16):

table 15: analysis of variance table

Cause of change	Sum of squares	Degree of freedom	Mean square	F value	Significance of
						Treatment room	100.4139	3	33.4713	133.885	Is remarkable in that
In-process	2	8	0.25
						Total variation	102.4139	11

Table 16: soil organic matter content result new complex pole difference method testing table

Treatment of	Mean value	5% significant level	1% very significant level
				Treatment
4	27.02	a	A
				Treatment
3	22.31	b	B
				Treatment
2	20.38	c	C
				Process
1	19.56	c	C

From table 15, it can be seen that F value (3, 8) ═ 133.885> F0.01(3, 8) ═ 7.59, i.e. the difference between different treatments is very significant, and the application of straw and cow dung ultra-high temperature organic fertilizer has obvious effect of improving the organic matter content of soil. On the level of difference between 1% and 5% (table 15), the organic matter content of the soil treated by the treatment 4 (applying straw and cow dung ultra-high temperature decomposed organic fertilizer) and the treatment 3 (applying commercial organic fertilizer) is obviously improved greatly compared with the treatment 2 using chemical fertilizer and the treatment 1 without applying fertilizer. The difference between the organic matter content of the soil treated by the treatment 1 and the organic matter content of the soil treated by the treatment 2 is not significant on the level of 5 percent and on the level of 1 percent, wherein the organic matter content of the soil subjected to the straw and cow dung ultrahigh-temperature decomposed organic fertilizer is the highest.

Randomly borrowing soil to detect the content of the hydrolyzed nitrogen in the soil, and analyzing the data by using a Duncan's new repolarization test method of DPS software (see tables 17 and 18):

table 17: analysis of variance table

Cause of change	Sum of squares	Degree of freedom	Mean square	F value	Significance of
						Treatment room	1785.751	3	595.2503	148.813	Is remarkable in that
In-process	32	8	4
						Total variation	1817.751	11

Table 18: soil hydrolysis nitrogen content result new repolarization difference method test table

Treatment of	Mean value	5% significant level	1% very significant level
				Treatment
4	173.6	a	A
				Treatment
3	158.2	b	B
				Treatment
2	145.9	c	C
				Process
1	142.5	c	C

From table 17, it can be seen that F value (3, 8) ═ 148.813> F0.01(3, 8) ═ 7.59, i.e. the difference between different treatments is very significant, and the application of straw and cow dung ultra-high temperature organic fertilizer has obvious effect of improving the content of soil hydrolysis nitrogen. At the level of difference between 1% and 5% (table 18), the nitrogen content of the soil hydrolysis in treatments 4 (application of straw and cow dung ultra-high temperature decomposed organic fertilizer) and 3 (application of commercial organic fertilizer) was significantly higher than in

treatments

2 and 1 with no fertilizer. The difference between the soil hydrolysis nitrogen content of the treatment 1 and the soil hydrolysis nitrogen content of the treatment 2 is not significant at a 5% level and at a 1% level, wherein the soil hydrolysis nitrogen content of the organic fertilizer which is decomposed at the ultrahigh temperature by applying straw and cow dung is the highest.

Randomly borrowing soil to detect the content of available phosphorus in soil, and analyzing the data by using a Duncan's new repolarization test method of DPS software (see tables 19 and 20):

table 19: analysis of variance table

Cause of change	Sum of squares	Degree of freedom	Mean square	F value	Significance of
						Treatment room	2204.55	3	734.85	18371.29	Is extremely remarkable
In-process	0.32	8	0.04
						Total variation	2204.87	11

Table 20: new complex pole difference method testing table for effective phosphorus content result

Treatment of	Mean value	5% significant level	1% very significant level
				Treatment
4	99.1	a	A
				Treatment
2	91.4	b	B
				Treatment
3	86.5	c	C
				Process
1	62.8	d	D

From table 19, it can be seen that F value (3, 8) ═ 18371.29> F0.01(3, 8) ═ 7.59, i.e. the difference between different treatments is very significant, and the application of straw and cow dung organic fertilizer has obvious effect of improving the content of available phosphorus in soil. At the level of difference between 1% and 5% (table 20), the effective phosphorus content of the soil treated by 4 (applied with straw and cow dung ultra-high temperature decomposed organic fertilizer) and 2 (applied with fertilizer) is obviously improved greatly compared with the treatment 3 with the commercial organic fertilizer and the treatment 1 without fertilizer. The yield of the rape treated by the treatment 1, the treatment 2, the treatment 3 and the treatment 4 is very different at a 5% level and a 1% level, wherein the effective phosphorus content of the ultrahigh-temperature decomposed organic fertilizer soil applied with straws and cow dung is the highest.

Randomly taking soil to detect the content of the quick-acting potassium in the soil, and analyzing the data by using a Duncan's new repolarization test method of DPS software (see tables 21 and 22):

table 21: analysis of variance table

Cause of change	Sum of squares	Degree of freedom	Mean square	F value	Significance of
						Treatment room	4950	3	1650	66	Is remarkable in that
In-process	200	8	25
						Total variation	5150	11

Table 22: soil quick-acting potassium content result new complex pole difference method testing table

Treatment of	Mean value	5% significant level	1% very significant level
				Treatment
4	136	a	A
				Treatment
3	115	b	B
				Process
1	94	c	C
				Treatment
2	83	c	C

From table 21, it can be seen that F value (3, 8) ═ 66> F0.01(3, 8) ═ 7.59, i.e. the difference between different treatments is very significant, and the application of straw and cow dung ultra-high temperature organic fertilizer has obvious effect of improving the content of quick-acting potassium in soil. At the level of difference between 1% and 5% (table 22), the soil quick-acting potassium content of the treated 4 (applied with straw and cow dung ultra-high temperature decomposed organic fertilizer) and the treated 3 (applied with commercial organic fertilizer) is obviously improved greatly compared with the treated 2 and non-applied 1 with fertilizers. The difference between the quick-acting potassium content of the soil in the treatment 1 and the soil in the treatment 2 is not significant at the level of 5 percent and at the level of 1 percent, wherein the quick-acting potassium content of the soil is the highest when the straw and cow dung are applied to the ultrahigh-temperature decomposed organic fertilizer.

The test results show that the straw and cow dung organic fertilizer obtained by the invention can replace chemical fertilizers, can obviously improve the yield and the quality of rape, and has obvious effects on the aspects of improving the utilization rate of nitrogen and improving the active organic matters of soil. Of course, the method can also be applied to cultivation of vegetables such as hot pepper, eggplant, Chinese cabbage and the like, and the effects are also achieved, but the method is not limited to the examples.

Claims

1. The utility model provides a straw and cow dung ultra-temperature decomposition agent, is dried after mixing fermentation by straw, cow dung and the compound microbial inoculum of becoming thoroughly decomposed and obtains its characterized in that: the decomposed complex microbial inoculum is composed of the following raw materials in parts by weight: 3-8 parts of thermophilic bacteria agent, 2-5 parts of bacillus licheniformis agent, 2-5 parts of aspergillus oryzae agent, 2-5 parts of lactobacillus plantarum agent, 2-5 parts of sugarcane orchid bacterium agent, 2-5 parts of cold-resistant brevibacterium agent,

the content of the thermophilic bacteria agent is 6-7 multiplied by 10¹¹cfu/g, wherein the thermophilic bacteria used for preparing the thermophilic bacteria agent is thermophilic bacteria (Therma bacterium composi) GW, and the preservation number is CGMCC No. 15721;

the content of the bacillus licheniformis agent is 4-5 multiplied by 10¹⁰cfu/g, the bacillus licheniformis used for preparing the bacillus licheniformis microbial agent is any one or more of bacillus licheniformis CICC10037, bacillus licheniformis CICC10084, bacillus licheniformis CICC10085 and bacillus licheniformis CICC 10092;

the content of Aspergillus oryzae microbial inoculum is 8-10 × 10¹⁰cfu/g, wherein the aspergillus oryzae used for preparing the aspergillus oryzae microbial inoculum is any one or more of aspergillus oryzae CICC2001, aspergillus oryzae CICC2011, aspergillus oryzae CICC2016 and aspergillus oryzae CICC 2022;

the lactobacillus plantarum microbial inoculum content is 8-9×10¹⁰cfu/g, the lactobacillus plantarum used for preparing the lactobacillus plantarum microbial inoculum is lactobacillus plantarum CGMCC 1.2437;

the content of the Langeria sacchari preparation is 7-8 multiplied by 10¹¹cfu/g, wherein the Langeria saccharensis used for preparing the Langeria saccharensis microbial inoculum is Lanceyerla sacchara (CP), and the preservation number is CGMCC No. 13928;

the content of the cold-resistant brevibacterium strain microbial inoculum is 3-5 multiplied by 10¹¹cfu/g, the Brevibacterium frigidum used for preparing the Brevibacterium frigidum microbial inoculum is Brevibacterium frigidum (Brevibacterium frigidariurans) NH, and the preservation number is CGMCC No. 15722.

2. The straw and cow dung ultrahigh-temperature decomposing agent as claimed in claim 1, wherein: the decomposed complex microbial inoculum is prepared from the following raw materials in parts by weight: 6 parts of a thermophilic bacteria agent, 4 parts of a bacillus licheniformis agent, 4 parts of an aspergillus oryzae agent, 4 parts of a lactobacillus plantarum agent, 4 parts of a lancina sacchari agent and 4 parts of a cold-resistant brevibacterium agent.

3. The straw and cow dung ultrahigh-temperature decomposing agent as claimed in claim 1 or 2, wherein:

the preparation method of the thermophilic bacteria agent comprises the following steps: inoculating thermophilic bacteria into high-temperature bacteria seed solid slant culture medium A, culturing at 60-90 deg.C for 18-22 hr, eluting to obtain eluted bacterial liquid, and adjusting bacterial concentration of the bacterial liquid to 4-6 × 10¹¹cfu/mL, inoculating the culture medium into the solid culture medium B according to the inoculation amount of 50-150mL/kg, mixing and stirring, standing and culturing at 60-90 ℃ for 16-20h, supplementing water for 1-3 times/day, drying and crushing to obtain the thermophilic bacteria agent;

the high-temperature bacterium seed solid culture medium A comprises the following components: 2-3g/L of yeast extract, 2-3g/L of bacterial peptone, 2-3g/L of glucose, 6-8g/L of PIPES, 8-12g/L of plant gel, 8-12g/L of agar powder and the balance of water, adjusting the pH value to 7.2-7.4, and sterilizing at 121 ℃ for 30 min;

the solid medium B comprises the following components: 100 portions of rice husk-containing material, 110 portions of bran-containing material, 100 portions of corn flour-containing material, 115 portions of bean cake powder, 100 portions of glucose-containing material, 2-3 portions of calcium sulfate, 2-4 portions of calcium oxide, 1-3 portions of dipotassium hydrogen phosphate and 0.5-1.2 portions of magnesium sulfate, wherein the weight ratio of the total amount of solid materials to water is as follows: 5-6:4-5 adding water into the solid material, and sterilizing at 121 deg.C for 45 min.

4. The straw and cow dung ultrahigh-temperature decomposing agent as claimed in claim 1 or 2, wherein: the preparation method of the bacillus licheniformis agent comprises the following steps: inoculating Bacillus licheniformis into NA seed solid culture medium C, standing at 30-40 deg.C for 1-2d, eluting to obtain eluate, and adjusting the concentration of the eluate to 3-5 × 10¹⁰cfu/mL, inoculating the solid culture medium D according to the inoculation amount of 50-120mL/kg culture medium, stirring and mixing, standing and culturing at 30-40 ℃ for 1-2D, and drying and crushing to obtain a bacillus licheniformis microbial agent;

the NA seed solid culture medium C comprises the following components: 10-12g/L of peptone, 3-4g/L of beef powder, 10-12g/L of sodium chloride, 18-20g/L of agar and the balance of water, adjusting the pH value to 7.0-7.2, and sterilizing at 121 ℃ for 30 min;

the solid medium D comprises the following components: 275 parts of 250-containing rice husk, 750 parts of 500-containing bran, 625 parts of 375-containing corn flour, 275 parts of 200-containing bean cake powder, 250 parts of 225-containing starch, 275 parts of 250-containing red soil, 50-62 parts of glucose, 12-15 parts of calcium sulfate, 15-20 parts of calcium oxide, 5-10 parts of dipotassium hydrogen phosphate and 5-6 parts of magnesium sulfate, wherein water is added into the solid materials according to the weight ratio of the total amount of the solid materials to the water of 7-15:2-5 for mixing, and the solid materials are sterilized for 45min at 121 ℃.

5. The straw and cow dung ultrahigh-temperature decomposing agent as claimed in claim 1 or 2, wherein:

the preparation method of the aspergillus oryzae microbial inoculum comprises the following steps: inoculating Aspergillus oryzae strain in PDA solid culture medium, standing at 25-30 deg.C for 5-6d, eluting to obtain eluate, and adjusting the concentration of the eluate to 2-4 × 10¹¹cfu/mL, inoculating the strain in a culture medium with an inoculation amount of 50-120mL/kg into a solid culture medium B, standing and culturing at 25-30 ℃ for 5-6 days, and drying and crushing to obtain the Aspergillus oryzae viable bacteria agent.

6. The straw and cow dung ultrahigh-temperature decomposing agent as claimed in claim 1 or 2, wherein:

the preparation method of the lactobacillus plantarum microbial inoculum comprises the following steps: inoculating Lactobacillus plantarum in seed solid culture medium C, standing at 30-40 deg.C for 2-3d, eluting to obtain eluate, and adjusting concentration to 4-5 × 10¹⁰cfu/mL, inoculating the bacterial liquid into a solid culture medium F according to the inoculation amount of 50-120mL/kg culture medium, standing and culturing for 2-3d at 30-40 ℃, and drying and crushing to obtain a lactobacillus plantarum microbial inoculum;

the preparation method of the solid culture medium F comprises the following steps: 500 parts of bean cake powder 300-.

7. The straw and cow dung ultrahigh-temperature decomposing agent as claimed in claim 1 or 2, wherein:

the preparation method of the Lanchow bacterium agent comprises the following steps: inoculating Hill-minck Saccharum in NA seed solid culture medium C, standing at 50-70 deg.C for 1-2d, eluting thallus to obtain eluate, and adjusting concentration to 5-5.5 × 10¹¹cfu/mL, inoculating the bacterial liquid into a solid culture medium A according to the inoculation amount of 50-120mL/kg culture medium, standing and culturing at 50-70 ℃ for 4-5 days, and drying and crushing to obtain the sugarcane orchid bacterium viable bacteria agent.

8. The straw and cow dung ultrahigh-temperature decomposing agent as claimed in claim 1 or 2, wherein:

the preparation method of the cold-resistant brevibacterium inoculant comprises the following steps: inoculating Brevibacterium frigidum in NA seed solid culture medium C, standing at 8-10 deg.C for 2-3d, eluting to obtain eluate, and adjusting concentration to 8-9 × 10¹⁰cfu/mL, inoculating the bacterial liquid into solid culture medium G according to the inoculum size of 50-110mL/kg, standing and culturing at 8-10 deg.C for 3-4d, drying, and pulverizingCrushing to obtain a cold-resistant brevibacterium inoculant;

the solid medium G comprises the following components: 620g/L of bran 600-.

9. The preparation method of the straw and cow dung ultrahigh-temperature decomposing agent as claimed in any one of claims 1 to 8, comprising the following steps:

(1) and (3) mixing the microbial inoculum: mixing a thermophilic bacteria agent, a bacillus licheniformis agent, an aspergillus oryzae agent, a lactobacillus plantarum agent, a sugarcane orchid bacterium agent and a cold-resistant brevibacterium agent according to the parts by weight to obtain a decomposed compound bacterium agent;

(3) inoculating the decomposed complex microbial inoculum into a mixed compost body according to the inoculation amount of 1-2 per mill of the mass ratio, stirring and mixing, carrying out decomposition treatment, and composting for 10-15d to obtain the straw and cow dung ultrahigh-temperature decomposing inoculant.

10. Use of the straw and cow dung ultrahigh-temperature decomposition agent according to any one of claims 1-8 in the field of fruit and vegetable planting.