CN113583919B - Solid composite microbial agent for improving cow dung fermentation effect and preparation method and application thereof - Google Patents
Solid composite microbial agent for improving cow dung fermentation effect and preparation method and application thereof Download PDFInfo
- Publication number
- CN113583919B CN113583919B CN202111038444.0A CN202111038444A CN113583919B CN 113583919 B CN113583919 B CN 113583919B CN 202111038444 A CN202111038444 A CN 202111038444A CN 113583919 B CN113583919 B CN 113583919B
- Authority
- CN
- China
- Prior art keywords
- mixture
- cow dung
- composite microbial
- improving
- solid composite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Fertilizers (AREA)
Abstract
The invention belongs to the technical field of composite microbial agents, and provides a solid composite microbial agent for improving cow dung fermentation effect, and a preparation method and application thereof. The invention mixes the mixture of bran and sawdust and glucose with high temperature resistant bacteria and low temperature resistant bacteria respectively to prepare the solid composite microbial inoculum capable of improving the cow dung fermentation effect. The prepared composite microbial inoculum is added into cow dung, so that the composting fermentation time is shortened, the composting efficiency and the quality of the organic fertilizer are improved, and the composite microbial inoculum has important significance in reducing environmental pollution, promoting crop growth and the like.
Description
Technical Field
The invention relates to the technical field of composite microbial agents, in particular to a solid composite microbial agent for improving the fermentation effect of cow dung, and a preparation method and application thereof.
Background
At present, according to investigation data of national agricultural rural areas, the annual output of livestock and poultry manure in China is increased year by year, and is expected to exceed 40 hundred million tons in 2022, and the manure is easy to breed pathogenic microorganisms, so that the manure is directly discharged too much, serious pollution is caused to the environment, the growth of crops is influenced, and the survival and the development of livestock and poultry farming industry are limited. Composting is called aerobic composting, which is to decompose substances which are not easy to decompose in the accumulated feces into substances which are beneficial to the environment and soil plants under the action of microorganisms. Composting is the most common mode for disposing excrement in farms, has the advantages of low processing cost, capability of killing pathogenic bacteria, effective degradation of organic pollutants and the like, is widely applied to northern provinces such as Hebei and inner Mongolia in China, but has low air temperature in autumn and winter in northern areas, so that composting fermentation is slow and low in efficiency, and smooth composting of cow dung under low-temperature conditions is a key problem to be solved, and meanwhile, difficult degradation substances such as cellulose and lignin in the cow dung are mainly decomposed in a high-temperature period, but most microorganisms cannot survive due to the fact that the temperature is too high.
With the rapid development of cattle raising industry, the pollution of cow dung to the environment is increasingly serious, and a composting method for realizing the resource utilization of cow dung is widely focused. When the ambient temperature is lower, the problems that the pile body is difficult to start, the pile body temperature cannot be increased, the manure cannot be fermented effectively and rapidly and the like occur in cow manure composting. Through a cow dung composting test, the solid composite microbial inoculum for improving the cow dung fermentation effect is developed, so that the heap temperature can be quickly improved, and the low-temperature start can be accelerated in autumn and winter, thereby achieving the purposes of quickly raising the initial heap temperature, prolonging the high-temperature period, shortening the fermentation period and improving the quality of the organic fertilizer and innocent treatment.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide a solid composite microbial inoculum capable of improving the fermentation efficiency of cow dung compost and improving the fermentation effect of cow dung, and a preparation method and application thereof.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a preparation method of a solid composite microbial inoculum for improving cow dung fermentation effect, which comprises the following steps:
(1) Mixing bran and sawdust to obtain a mixture A;
(2) Mixing the mixture A with glucose and high temperature resistant bacteria to obtain a mixture B;
(3) Mixing the mixture A with glucose and low temperature resistant bacteria to obtain a mixture C;
(4) And mixing the mixture B and the mixture C to obtain the solid composite microbial inoculum capable of improving the cow dung fermentation effect.
Preferably, in the step (1), the mass ratio of the bran to the sawdust is 1:0.5-1.5.
Preferably, the mass ratio of the mixture A in the step (2) to the mixture A in the step (3) is 3-5:11-13.
Preferably, in the step (2), the dosage ratio of the mixture A to glucose to the high temperature resistant bacteria is 350-450 g to 50-150 g to 350-450 mL;
the high temperature resistant bacteria consist of streptococcus thermophilus ACCC10651 and bacillus licheniformis ACCC19372 according to the volume ratio of 1:0.5-1.5.
Preferably, in the step (3), the dosage ratio of the mixture A, the glucose and the low-temperature resistant bacteria is 1100-1300 g:250-350 g:1100-1300 mL;
the low temperature resistant bacteria are composed of bacillus licheniformis ACCC 0172, bacillus licheniformis ACCC01958, bacillus megaterium ACCC02979, bacillus megaterium ACCC01997, bacillus cereus ACCC01547 and bacillus thuringiensis ACCC04323 according to the volume ratio of 1:0.5-1.5:0.5-1.5.
Preferably, in the step (4), a step of drying the mixture B and the mixture C is further included before the mixing, wherein the drying temperature of the mixture B is 50-60 ℃, the drying temperature of the mixture C is 20-30 ℃, and the drying is performed until the weight is constant.
The invention also provides a solid composite microbial inoculum for improving the cow dung fermentation effect.
The invention also provides application of the solid composite microbial inoculum for improving the cow dung fermentation effect in the cow dung composting fermentation process.
Preferably, the inoculation amount of the solid composite microbial inoculum for improving the cow dung fermentation effect is 0.90-0.95% of the total mass of cow dung compost, the fermentation temperature is 45-55 ℃, and the fermentation time is 5-7 days.
The beneficial effects of the invention are as follows:
according to the test, the solid composite microbial inoculum prepared by mixing the high temperature resistant bacteria and the low temperature resistant bacteria is added into cow dung, so that the composting fermentation time is shortened, the composting efficiency and the quality of the organic fertilizer are improved, and the test has important significance in reducing environmental pollution, promoting crop growth and the like.
Drawings
FIG. 1 is a graph showing temperature changes during composting;
fig. 2 is a graph showing the change in pH during composting (indicating significant differences);
fig. 3 shows the change in water content during composting (indicating significant differences);
fig. 4 shows the change in C/N during composting (x indicates significant difference; x indicates very significant difference);
fig. 5 shows the change in organic matter during composting (indicating significant difference; indicating very significant difference);
FIG. 6 is a variation of total phosphorus during composting;
FIG. 7 is a graph showing the variation of total potassium during composting;
fig. 8 shows changes in GI during composting (x represents significant differences; x represents very significant differences);
FIG. 9 is a graph of Alpha index sparseness;
FIG. 10 is a portal horizontal species composition;
FIG. 11 is a generic horizontal species composition.
Detailed Description
The invention provides a preparation method of a solid composite microbial inoculum for improving cow dung fermentation effect, which comprises the following steps:
(1) Mixing bran and sawdust to obtain a mixture A;
(2) Mixing the mixture A with glucose and high temperature resistant bacteria for fermentation to obtain a mixture B;
(3) Mixing the mixture A with glucose and low temperature resistant bacteria for fermentation to obtain a mixture C;
(4) And mixing the mixture B and the mixture C to obtain the solid composite microbial inoculum capable of improving the cow dung fermentation effect.
In the present invention, in the step (1), the mass ratio of the bran to the sawdust is preferably 1:0.5-1.5, and more preferably 1:1.
In the present invention, the mass ratio of the mixture A in the step (2) to the step (3) is preferably 3 to 5:11 to 13, and more preferably 1:3.
In the present invention, in the step (2), the dosage ratio of the mixture A, glucose and the thermophilic bacteria is preferably 350-450 g, 50-150 g, 350-450 mL, and more preferably 400g, 100g, 400mL;
the high temperature resistant bacteria are preferably composed of streptococcus thermophilus ACCC10651 and bacillus licheniformis ACCC19372 according to the volume ratio of 1:0.5-1.5, and more preferably according to the volume ratio of 1:1.
In the present invention, in the step (3), the dosage ratio of the mixture A, glucose and the low temperature resistant bacteria is preferably 1100-1300 g, 250-350 g, 1100-1300 mL, and more preferably 1200g, 300g, 1200mL;
the low temperature resistant bacteria are preferably composed of bacillus licheniformis ACCC01172, bacillus licheniformis ACCC01958, bacillus megaterium ACCC02979, bacillus megaterium ACCC01997, bacillus cereus ACCC01547 and bacillus thuringiensis ACCC04323 according to the volume ratio of 1:0.5-1.5:0.5-1.5, and more preferably according to the volume ratio of 1:1:1:1:1:1:1.
In the present invention, the step (4) preferably further comprises a step of drying the mixture B and the mixture C before the mixing, wherein the drying temperature of the mixture B is preferably 50 to 60 ℃, more preferably 55 ℃, the drying temperature of the mixture C is preferably 20 to 30 ℃, more preferably 25 ℃, and the drying is preferably performed to a constant weight.
The invention also provides a solid composite microbial inoculum for improving the cow dung fermentation effect.
The invention also provides application of the solid composite microbial inoculum for improving the cow dung fermentation effect in the cow dung composting fermentation process.
In the invention, the inoculation amount of the solid composite microbial inoculum for improving the cow dung fermentation effect is preferably 0.90-0.95% of the total mass of cow dung compost, more preferably 0.94% of the total mass of cow dung compost, the fermentation temperature is preferably 45-55 ℃, more preferably 50 ℃, and the fermentation time is preferably 5-7 days, more preferably 6 days.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
The thermophilic bacteria (Streptococcus thermophilus ACCC10651, bacillus licheniformis ACCC 19372) and the thermophilic bacteria (Bacillus licheniformis ACCC01172, bacillus licheniformis ACCC01958, bacillus megaterium ACCC02979, bacillus megaterium ACCC01997, bacillus cereus ACCC01547, bacillus thuringiensis ACCC 04323) in the following examples were purchased from China center for type culture collection of agricultural microorganisms.
Example 1
Mixing bran and sawdust to obtain a mixture A;
mixing 350g of the mixture A with 50g of glucose and 350mL of thermophilic bacteria to obtain a mixture B;
mixing 1100g of the mixture A with 250g of glucose and 1100mL of low temperature resistant bacteria to obtain a mixture C;
and (3) drying the mixture B at 50 ℃, drying the mixture C at 20 ℃ to constant weight, and mixing the dried mixture B with the mixture C to obtain the solid composite microbial inoculum capable of improving the cow dung fermentation effect.
Example 2
Mixing bran and sawdust to obtain a mixture A;
mixing 400g of the mixture A with 100g of glucose and 400mL of thermophilic bacteria to obtain a mixture B;
mixing 1200g of the mixture A with 300g of glucose and 1200mL of low temperature resistant bacteria to obtain a mixture C;
and (3) drying the mixture B at 55 ℃, drying the mixture C at 25 ℃ to constant weight, and mixing the dried mixture B with the mixture C to obtain the solid composite microbial inoculum capable of improving the cow dung fermentation effect.
Example 3
Mixing bran and sawdust to obtain a mixture A;
mixing 450g of the mixture A with 150g of glucose and 450mL of thermophilic bacteria to obtain a mixture B;
1300g of the mixture A is mixed with 350g of glucose and 1300mL of low temperature resistant bacteria to obtain a mixture C;
and (3) drying the mixture B at 60 ℃, drying the mixture C at 30 ℃ to constant weight, and mixing the dried mixture B with the mixture C to obtain the solid composite microbial inoculum capable of improving the cow dung fermentation effect.
Taking the solid composite microbial inoculum for improving the fermentation effect of cow dung obtained in the example 2 as an example, the following experiment is carried out:
the cow dung in the following experiments is fresh cow dung with the moisture content of 60% after dry-wet separation and is provided by a Hebei agricultural university test cow farm;
experimental grouping:
t groups: uniformly mixing the composite microbial inoculum obtained in the example 2 with cow dung, wherein the inoculation amount of the composite microbial inoculum is 0.94%;
group C: equal amounts of bran, sawdust and glucose were mixed with cow dung.
The 12 months in 2020 are established for 22 days, the day of composting is recorded as 1d, the time sampling is carried out, the sampling time of physical and chemical indexes is 0, 5, 10, 15 and 20d, the test group is set as a T group, and the control group is set as a C group. The high-throughput sequencing sampling time is five periods of initial composting (T1 and C1), heating period (T2 and C2), high-temperature period (T3 and C3), cooling period (T4 and C4) and decomposing period (T5 and C5), samples are respectively taken from four corners and central parts (15 cm, 30cm and 45 cm) of a pile body, samples of different parts in the same period are uniformly mixed, one part is preserved at-20 ℃ for physical and chemical index detection, and the other part is preserved at-80 ℃ for high-throughput sequencing analysis of species composition and dominant microorganisms in each stage.
Experimental example 1
The effect of the composite microbial agent obtained in example 2 on composting temperature was studied: once per day at 9:00 and 17:00, all samples were measured for average as the stack temperature on the day and room temperature was measured. The results are shown in FIG. 1.
The composting process requires 22d, and as can be seen from fig. 1, the composting temperature change trend of the test group (T group) and the control group (C group) is basically consistent, and the composting temperature change trend is firstly increased and then decreased. However, group T and group C, group 1d stack temperatures were 28.3 and 21.0 ℃, respectively; the temperature of the T group rises rapidly, the temperature reaches a high temperature stage two days earlier than that of the C group, the temperature of the 5 th d entering the high temperature stage is 55.3 ℃, the 8 th d reaching the highest composting temperature of 59.7 ℃, and the high temperature stage lasts for 7d; the temperature of the group C7 d is 55.3 ℃, the 9d reaches the highest temperature of 56.3 ℃, and the high-temperature stage lasts for 5d, so that the composition basically accords with the 'excrement harmless sanitation standard' (GB 7959-87) in China. It can be seen that the addition of the composite microbial inoculum of example 2 can rapidly initiate the initial temperature of composting, raise the highest temperature of composting and prolong the high temperature time compared with the control group.
Experimental example 2
The effect of the complex inoculant obtained in example 2 on compost pH was studied: measured using a pH meter. The results are shown in FIG. 2.
As can be seen from FIG. 2, the pH of the compost is first raised and then lowered. The initial pH value is about 8.0, the pH value of the test group is obviously increased (p is less than 0.05) compared with that of the control group at the 5d along with the composting process, and the test group and the control group have no obvious difference in other time periods. The pH 10d reaches a maximum value, and then the pH is reduced all the time, and at the end, the pH is reduced to a minimum value, and the pH is maintained between 8.0 and 8.5. It can be seen that the addition of the complex microbial agent of example 2 increases the pH change compared to the control group.
Experimental example 3
The effect of the composite microbial agent obtained in example 2 on the water content of compost was studied: the measurement was carried out by a 105℃oven-drying method. The results are shown in FIG. 3.
As can be seen from FIG. 3, the initial water content of the compost in the test group (T group) and the control group (C group) is 60.0%, and the change trend of the water content of the compost in the two groups is basically consistent. At the end of composting, the water content of group C is reduced by 18.4%, the final water content of group T is reduced by 24%, and the two groups are obviously different at 15 d. It can be seen that the addition of the composite microbial agent of example 2 can promote a decrease in moisture content during the composting process of cow dung compared with the control group.
Experimental example 4
The effect of the composite inoculant obtained in example 2 on C/N during composting was studied: total organic carbon determination: reference is made to the national standard GB13193-91; total nitrogen determination: reference is made to the agricultural industry standard NYT 297-1995. The results are shown in FIG. 4.
As can be seen from FIG. 4, the C/N change rule in the composting process is always reduced, the initial cow dung C/N is not significantly different, the test group is significantly reduced (P < 0.01) compared with the control group at the time of 5d, and the test group is significantly reduced (P < 0.05) compared with the control group in the rest period. The initial C/N values of the experiment are 32.0 and 32.4 for the C group and the T group, and 16.4 and 15.1 for the C/N groups at the end of composting. It can be seen that the addition of the complex microbial inoculant of example 2 resulted in a faster C/N drop during composting compared to the control.
Experimental example 5
The effect of the composite microbial inoculum obtained in example 2 on organic matters in the composting process is studied: organic matter measurement: organic carbon x 1.724. The results are shown in FIG. 5.
As can be seen from fig. 5, the organic matter content in the composting process tends to decrease slowly. There was no significant difference between group T and group C at 0d and 20d, the T group was significantly reduced (P < 0.01) compared to group C at 5d and 10d, and the T group was significantly reduced (P < 0.05) compared to group C at 15 d. The initial contents of organic matters in the group C and the group T are 682.3 g and 682.5g.kg respectively -1 The final contents were 567.7 and 559g.kg, respectively -1 Respectively 114.6 and 123.5g.kg -1 A large amount of organic matters which are easy to decompose and contained in the early-stage composting cow dung are rapidly decomposed and rapidly reduced. It can be seen that the addition of the composite microbial inoculum of example 2 can promote the decomposition of organic matters in the compost compared with the control group.
Experimental example 6
The effect of the composite microbial inoculum obtained in example 2 on total phosphorus during composting was studied: total phosphorus determination: reference is made to the agricultural industry standard NYT 298-1995. The results are shown in FIG. 6.
As can be seen from fig. 6, the total phosphorus content tends to increase during composting. There was no significant difference between group T and group C at 0d, significant increase between group T and group C at 5d (P < 0.05), and very significant increase between group T and group C at 10d, 15d and 20d (P < 0.01). Initial contents of group C and group T were 54.4 and 55.3g.kg, respectively -1 Final contents were 63.1 and 72.4g.kg, respectively -1 . It can be seen that the addition of the composite microbial inoculum of example 2 increased the total phosphorus content during composting compared to the control group.
Experimental example 7
The effect of the composite microbial inoculum obtained in example 2 on total potassium in the composting process was studied: full potassium determination: reference is made to the agricultural industry standard NYT 299-1995. The results are shown in FIG. 7.
As can be seen from FIG. 7, the total potassium content was in an upward trend during composting. Initial contents of both group C and group T were 7.4g.kg -1 Final content of 11.0 and 11.6g.kg respectively -1 . As can be seen, the complex bacteria of example 2 were added during composting compared to the control groupThe agent can slightly increase the total potassium content.
Experimental example 8
The effect of the composite microbial agent obtained in example 2 on the germination index GI of seeds was studied: GI determination was carried out by the method described in "Wang Xue" development and application research of novel biological organic fertilizer for livestock and poultry feces ". The results are shown in FIG. 8.
As can be seen from fig. 8, GI tends to rise during composting. There was no significant difference between the T group at 0d and the C group, and the T group was significantly elevated (P < 0.01) at 5d, 10d, 15d and 20d compared to the C group. Initial GI of group C and T are 26% and 26.3%, final GI is 88.2% and 92.7%, respectively, and the rising amplitude of the two groups 0-10d is maximum, which indicates that the high temperature period plays a key role on GI, and the GI of the two groups at the end of composting is over 80%, which can be regarded as that the cow dung has no phytotoxicity or the cow dung has decomposed. It can be seen that the addition of the complex microbial agent of example 2 increased the germination index of seeds compared to the control group.
Experimental example 9
The complex microbial agents obtained in example 2 were subjected to high throughput analysis:
data preprocessing: performing analysis of variance by adopting a single factor (ANOVA) method and an LSD method, wherein the analysis of variance adopts SPSS software, a histogram and a line drawing adopt GraphPad software, and dilution curve, species composition and dominant microorganism analysis adopt software QIIME2;
species composition and dominant microorganisms: 16S rRNA sequencing was performed using the Illumina platform Miseq, shanghai, north Bio Inc. And analyzing the species composition and dominant bacterial colony by detecting the effective data of each sample on the machine.
The smoothness degree of the dilution curve reflects the influence of the sequencing depth on the diversity of the observed sample, and the curve is flatter, so that the sequencing result is enough to reflect the diversity contained in the current sample, and a large number of undiscovered new OTUs can not be detected by continuously increasing the sequencing depth; otherwise, it indicates that alpha diversity is not approaching saturation. The results are shown in FIG. 9. As can be seen from FIG. 9, the curves C1 and T1 are lowest, gradually rising in sequence, and highest are C5 and T5. It can be seen that the sequencing amount of this test meets the level of diversity composition reflecting the cow dung flora in this test.
The composition of the species and dominant microorganisms in group C and T portal horizontal cow dung compost samples are shown in figure 10. A total of 31 gates were detected and only the top 10 with the highest abundance are shown in fig. 10. The species composition mainly comprises: proteus (Proteus), bacteroides (Bacteroides), fimbricus (Firmides), actinomycetes (Actinobacteria), chlorella (Chloroflexi), thermi, mortierella (Teneriicus) and Microwart (Verrucom). The variation rule of the various flora diversity is basically consistent between the group C and the group T, but the abundance ratio is greatly different. Proteobacteria are dominant microorganisms in two groups at each stage, and the ratio of the Proteobacteria to the Proteobacteria is 58.0% and is not quite different; the Bactoides exist in various stages, but the abundance ratio in the high-temperature period is highest, and the abundance ratio of C3 and T3 is 32.5% and 35.5% respectively; firmics hardly exists at the end of composting, and is dominant microorganism in the early stage (C1, T1) and the heating period (C2, T2), and the abundance of C1, T1, C2 and T2 is 17.9%, 24.4%, 13.0% and 14.4% respectively; actinomycetes are dominant microorganisms in the initial stage, the cooling period (C4 and T4) and the decomposing period (C5 and T5), and have small differences of about 8.0 percent of abundance ratio; chloroflex is dominant microorganism in cooling period and decomposing period, and abundance of C4, T4, C5 and T5 is 11.2%, 12%, 13.3% and 20.3% respectively. Compared with a control group, the composite microbial agent of the embodiment 2 is added to change the growth and propagation conditions of bacteria, change the abundance ratio of each strain in different composting stages and increase the number of dominant microorganisms.
The composition of the species and dominant microorganisms in group C and group T horizontal cow dung compost samples are shown in fig. 11. A total of 41 genera were detected and only the top 10 are shown in fig. 11. The species composition mainly comprises: other bacteria (other), pseudomonas (Pseudomonas), actinobacillus (Acinetobacter), psychrophilic (Psychrobacter), filamentous bacterium (Ruminophilibacter), cellvibrio (Cellvibrio), thermus (Aequorea), garcinia (Luteimonas), B-42 and Corynebacterium (Corynebacterium). Except that other is the highest, dominant microorganisms in the Pseudomonas warming period have C2 and T2 abundances of 22.0% and 23.0%, respectively; acinetobacter, psychrobacter and Corynebacterium are the primary dominant microorganisms with C1 and T1 abundances of 10.1%, 23.4%, 17.0%, 15.0%, 4.9% and 5.2%, respectively; cellvirio is the dominant microorganism abundance in the high temperature phase of T36.0% and C35.0%; the abundance of dominant microorganisms in the Ruminofilibacter cooling and decomposing periods increases from 1.0% to 3.7% in C5 and from 7.0% to 10.0% in T5; the ratio of the xanthomonas (Luteimonas) to the B-42 is small in each group of stages, but dominant microorganisms in the high temperature period and the cooling period can still be seen. It can be seen that the addition of the complex microbial agent of example 2 before composting changed the abundance ratio of each microorganism in different periods of composting compared to the control group, resulting in an increase in the number of dominant microorganisms.
In conclusion, the solid composite microbial inoculum for improving the fermentation effect of the cow dung prepared by the invention is added into cow dung compost, so that the composting time can be effectively shortened, and the composting efficiency and the quality of the organic fertilizer can be improved. High throughput sequencing has found that composting temperature and microbial number are key to composting success. The composite microbial inoculum provided by the invention can rapidly start the initial temperature of composting, improve the highest temperature of composting and prolong the high-temperature time, can change the growth and propagation conditions of bacteria, and change the abundance ratio of each strain in different composting stages, so that the number of dominant microorganisms is increased.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (6)
1. The preparation method of the solid composite microbial inoculum for improving the fermentation effect of the cow dung is characterized by comprising the following steps of:
(1) Mixing bran and sawdust to obtain a mixture A;
(2) Mixing the mixture A with glucose and high temperature resistant bacteria to obtain a mixture B; the dosage ratio of the mixture A to glucose to the high temperature resistant bacteria is 350-450 g, 50-150 g and 350-450 mL; the high temperature resistant bacteria is prepared from streptococcus thermophilusStreptococcus thermophilus) ACCC10651 and Bacillus licheniformisBacillus licheniformis) ACCC19372 is composed according to the volume ratio of 1:0.5-1.5；
(3) Mixing the mixture A with glucose and low temperature resistant bacteria to obtain a mixture C; the dosage ratio of the mixture A to glucose to the low-temperature resistant bacteria is 1100-1300 g to 250-350 g to 1100-1300 mL; the low temperature resistant bacteria are prepared from bacillus licheniformisBacillus licheniformis) ACCC01172 and Bacillus licheniformisBacillus licheniformis) ACCC01958 and Bacillus megateriumBacillus megaterium) ACCC02979 and Bacillus megateriumBacillus megaterium) ACCC01997, bacillus cereusBacillus cereus) ACCC01547 and Bacillus thuringiensisBacillus thuringiensis) ACCC04323 is formed by the volume ratio of 1:0.5-1.5:0.5-1.5;
(4) And mixing the mixture B and the mixture C to obtain the solid composite microbial inoculum capable of improving the cow dung fermentation effect.
2. The method for preparing the solid composite microbial inoculum for improving the fermentation effect of cow dung according to claim 1, wherein in the step (1), the mass ratio of bran to sawdust is 1:0.5-1.5.
3. The method for preparing the solid composite microbial inoculum for improving the cow dung fermentation effect according to claim 2, wherein the step (4) further comprises the step of drying a mixture B and a mixture C before mixing, wherein the drying temperature of the mixture B is 50-60 ℃, the drying temperature of the mixture C is 20-30 ℃, and the drying is carried out until the weight is constant.
4. The solid composite microbial inoculum for improving the fermentation effect of cow dung, which is prepared by the preparation method according to any one of claims 1-3.
5. The use of the solid composite microbial inoculant for improving the fermentation effect of cow dung in the fermentation process of cow dung compost.
6. The application of the solid composite microbial agent for improving the cow dung fermentation effect in the cow dung composting fermentation process, which is characterized in that the inoculation amount of the solid composite microbial agent for improving the cow dung fermentation effect is 0.90-0.95% of the total mass of the cow dung compost, the fermentation temperature is 45-55 ℃, and the fermentation time is 5-7 days.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111038444.0A CN113583919B (en) | 2021-09-06 | 2021-09-06 | Solid composite microbial agent for improving cow dung fermentation effect and preparation method and application thereof |
| PCT/CN2022/093448 WO2023029597A1 (en) | 2021-09-06 | 2022-05-18 | Solid compound bacterial agent for improving cow dung fermentation effect, preparation method therefor and application thereof |
| ZA2022/06252A ZA202206252B (en) | 2021-09-06 | 2022-06-06 | A solid compound microbial inoculum for improving the effect of cow dung fermentation and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111038444.0A CN113583919B (en) | 2021-09-06 | 2021-09-06 | Solid composite microbial agent for improving cow dung fermentation effect and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113583919A CN113583919A (en) | 2021-11-02 |
| CN113583919B true CN113583919B (en) | 2023-09-01 |
Family
ID=78241238
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111038444.0A Active CN113583919B (en) | 2021-09-06 | 2021-09-06 | Solid composite microbial agent for improving cow dung fermentation effect and preparation method and application thereof |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN113583919B (en) |
| WO (1) | WO2023029597A1 (en) |
| ZA (1) | ZA202206252B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113583919B (en) * | 2021-09-06 | 2023-09-01 | 河北农业大学 | Solid composite microbial agent for improving cow dung fermentation effect and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104945136A (en) * | 2015-06-26 | 2015-09-30 | 云南福湾农业科技有限公司 | Microbial organic substrate fermenting material and method for manufacturing organic substrate by means of same |
| CN105154373A (en) * | 2015-10-08 | 2015-12-16 | 山东大学 | High-temperature cow dung decomposition agent and preparation method thereof |
| CN108624539A (en) * | 2018-06-08 | 2018-10-09 | 河北木美土里科技有限公司 | A kind of stalk and cow dung superhigh temperature decomposing agent and its preparation and application |
| CN111849804A (en) * | 2020-06-11 | 2020-10-30 | 太仓绿丰农业资源开发有限公司 | High-temperature-resistant composite microbial agent for degrading livestock and poultry manure and application thereof |
| CN112795516A (en) * | 2021-02-07 | 2021-05-14 | 天津科技大学 | Complex microbial inoculant for decomposing and fermenting chicken manure compost |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101993832A (en) * | 2009-08-10 | 2011-03-30 | 北京农学院 | Composite bacterial agent for pig manure compost treatment and application method |
| CN107177521A (en) * | 2016-03-09 | 2017-09-19 | 北京大伟嘉生物技术股份有限公司 | A kind of composite bacteria agent for the organic fertilizer that ferments and its preparation method and application |
| CN111423994B (en) * | 2020-03-24 | 2021-03-05 | 北京北农企业管理有限公司 | Microbial inoculum combination applied to livestock and poultry manure fermentation and preparation and use methods thereof |
| CN113583919B (en) * | 2021-09-06 | 2023-09-01 | 河北农业大学 | Solid composite microbial agent for improving cow dung fermentation effect and preparation method and application thereof |
-
2021
- 2021-09-06 CN CN202111038444.0A patent/CN113583919B/en active Active
-
2022
- 2022-05-18 WO PCT/CN2022/093448 patent/WO2023029597A1/en unknown
- 2022-06-06 ZA ZA2022/06252A patent/ZA202206252B/en unknown
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104945136A (en) * | 2015-06-26 | 2015-09-30 | 云南福湾农业科技有限公司 | Microbial organic substrate fermenting material and method for manufacturing organic substrate by means of same |
| CN105154373A (en) * | 2015-10-08 | 2015-12-16 | 山东大学 | High-temperature cow dung decomposition agent and preparation method thereof |
| CN108624539A (en) * | 2018-06-08 | 2018-10-09 | 河北木美土里科技有限公司 | A kind of stalk and cow dung superhigh temperature decomposing agent and its preparation and application |
| CN111849804A (en) * | 2020-06-11 | 2020-10-30 | 太仓绿丰农业资源开发有限公司 | High-temperature-resistant composite microbial agent for degrading livestock and poultry manure and application thereof |
| CN112795516A (en) * | 2021-02-07 | 2021-05-14 | 天津科技大学 | Complex microbial inoculant for decomposing and fermenting chicken manure compost |
Non-Patent Citations (1)
| Title |
|---|
| 高通量测序分析发酵牛粪细菌和真菌的组成及多样性变化;岳世林等;《黑龙江畜牧兽医》;第631卷(第19期);全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113583919A (en) | 2021-11-02 |
| WO2023029597A1 (en) | 2023-03-09 |
| ZA202206252B (en) | 2022-06-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Beffa et al. | Taxonomic and metabolic microbial diversity during composting | |
| Insam et al. | Microbiology of the composting process | |
| Taiwo et al. | Influence of composting techniques on microbial succession, temperature and pH in a composting municipal solid waste | |
| Wang et al. | Influence and characteristics of Bacillus stearothermophilus in ammonia reduction during layer manure composting | |
| CN107141047B (en) | Composting method for promoting decomposition of livestock and poultry manure through damp-heat pretreatment | |
| CN106190927B (en) | A kind of bacterial strain and its application for sludge high temperature compost | |
| Xu et al. | Effect of inoculation with newly isolated thermotolerant ammonia-oxidizing bacteria on nitrogen conversion and microbial community during cattle manure composting | |
| CN107177533A (en) | A kind of Thermophilic Bacteria compounding microbial inoculum and preparation method and application | |
| CN102010824A (en) | Microbial agent for promoting quick decomposition of agricultural wastes and preparation method | |
| Qu et al. | Effect of EM microbial agent on aerobic composting for dairy cattle manure | |
| CN113583919B (en) | Solid composite microbial agent for improving cow dung fermentation effect and preparation method and application thereof | |
| CN111471617A (en) | Cellulose degradation composite flora and screening thereof and application of saprophytic fungi residue for decomposing | |
| CN105543149A (en) | Novel bacillus megaterium and application thereof | |
| CN109665876B (en) | Method for reducing abundance of macrolide drug-resistant genes in livestock and poultry manure | |
| CN111925964B (en) | Bacillus megaterium and application thereof | |
| CN104988095A (en) | Thermophilic carbon monoxide streptomycete low-temperature subspecies Dstr3-3 and application thereof | |
| CN115786163B (en) | Bacillus licheniformis SCAU1602 and application thereof | |
| Niu et al. | Effects of different microbial agents on bedding treatment of ectopic fermentation of buffalo manure | |
| Amanullah | Nutrient release pattern during composting poultry manure | |
| CN114317323A (en) | Microbial agent for rapidly degrading organic matters in kitchen waste | |
| CN110257486B (en) | Method for characterizing compost maturity based on cellulase gene | |
| Rajbanshi et al. | Stabilization of chemical and biochemical characteristics of grass straw and leaf mix during in-vessel composting with and without seeding material | |
| HU227106B1 (en) | Compost-making method from communal waste wather sludge | |
| CN107986820B (en) | Deodorizing additive for cow dung strip stack type compost and preparation method thereof | |
| Iresha et al. | Macronutrient content of compost produced by earthworm in the utilization of livestock waste using vermicomposting method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |