CN106045701A - Compound fertilizer prepared from waste through fermentation with threonine - Google Patents
Compound fertilizer prepared from waste through fermentation with threonine Download PDFInfo
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- CN106045701A CN106045701A CN201610396382.3A CN201610396382A CN106045701A CN 106045701 A CN106045701 A CN 106045701A CN 201610396382 A CN201610396382 A CN 201610396382A CN 106045701 A CN106045701 A CN 106045701A
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- fermentation
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- wheat stalk
- threonine
- fertilizer
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Fertilizers (AREA)
Abstract
The invention belongs to the field of treatment of fermentation wastewater in the biological fermentation industry and provides compound fertilizer prepared from waste through fermentation with threonine. The preparation of the compound fertilizer comprises steps as follows: filtering of the waste, hydrolysis, preparation of fermentation broth and freeze drying. The prepared compound fertilizer has the effects of nitrogen fixation and water retention, a lasting fertilizer effect and a certain weeding function and is environment-friendly and pollution-free, and both yield and revenue of crops can be increased.
Description
Technical field
The invention belongs to biofermentation industry Wastewater treating field, be specifically related to one and utilize Threonine Fermentation to discard
Fertilizer prepared by thing.
Background technology
Threonine is a kind of essential amino acid, is the second limiting amino acid and the 3rd restriction of poultry feed of pig feed
Aminoacid, along with the extensive application in mixed feed of lysine, methionine composite, threonine is increasingly becoming affects poultry life
Long major limiting factors.Threonine is mainly used as feed additive, and its production stage is for concentrating, crystallize, extracting thalline egg
In vain, and the tropina produced during it and mother liquid disposal difficulty are relatively big, relatively costly, it is difficult to obtain the pair that sexual valence is higher
Product.Patented technology " a kind of fertilizer utilizing Threonine Fermentation garbage to prepare " before applicant uses hydrolysis tropina
Mode prepare amino acid chelate fertilizer, but the method contains metal ion and is not suitable for preparing bacterial manure.
The development of China's fertilizer and application history, be to be Main Stage from farm manure to using inorganic fertilizer, because farm manure is dirty
Dye source is many, and freight volume is big, operationally wastes time and energy and effect is not the most obvious；And inorganic fertilizer composition is single, utilization rate
Relatively low, and often cause soil compaction and with soil erosion polluted source, influence ecological environment.At present, market is adopted more
With the inorganic composite fertilizer etc. of Nitrogen-and Phosphorus-containing potassium, the fertilizer efficiency of this compound fertilizer is comprehensive, but it is not lasting to there is also fertilizer efficiency, and easy loss etc. lacks
Fall into；The more important thing is, the price of current compound fertilizer is higher, brings bigger burden to peasant, how to reduce fertilizer cost, improves
Farmers' income is the agricultural modernization technical issues that need to address.
Summary of the invention
In order to overcome the defect of prior art, the invention provides the Chemical Mixed Fertilizer utilizing Threonine Fermentation garbage to prepare,
This Chemical Mixed Fertilizer is effectively utilized amino acid fermentation garbage, reduces fertilizer production cost, it is achieved that turn waste into wealth, and fertilizer is fertile
Effect persistently, has both weeding function.
For achieving the above object, the present invention is by the following technical solutions:
Utilizing Chemical Mixed Fertilizer prepared by Threonine Fermentation garbage, it comprises the steps:
The Threonine Fermentation garbage that biofermentation threonine is produced by step 1) filters, and collects tropina and waste water；
Step 2) tropina to be dried, pulverizer is ground into powder, is subsequently placed in retort, adds the salt of 5mol/L
Acid, not have raw material to be as the criterion, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, uses after reaction terminating
With remaining hydrochloric acid in ammonia, the pH controlling solution is 6.8-7.2；
Step 3) trichoderma aureoviride, Pseudomonas aeruginosa, candidiasis are cultivated respectively to concentration be 1 × 107The bacterium of individual/ml
Liquid, then according to the volume ratio mixing of 3:2:2, obtains composite bacteria agent capable A；
Step 4) arthrobacterium, bacillus megaterium, azotobacter chroococcum are cultivated respectively to concentration be 1 × 108The bacterium solution of individual/ml,
Then according to the volume ratio mixing of 2:2:1, obtain composite bacteria agent capable B；
Wheat stalk is ground into wheat stalk powder by step 5), adds step 2 to) solution in, stir, stand 12 little
Time, add bean cake, distiller grains, conch meal, potassium dihydrogen phosphate and glucose the most successively, 500 revs/min are stirred 3 minutes, then with
The programming rate of 5 DEG C/min is heated to 100 DEG C, is then incubated 30min, naturally cools to room temperature, according to 10%(v/v) inoculation
Amount inoculating compound bacterium agent A, controls at pH6.8-7.2, and temperature is that the condition of 28-30 DEG C is fermented 36 hours, obtains fermentation liquid A；
Wheat stalk is pulverized and is obtained wheat stalk powder by step 6), then equal with Testa Tritici, rapeseed meal and fishbone powder mix and blend
The even compound that obtains, adds the waste water of the step 1) accounting for compound twice quality, soaks 12 hours, then boil 15min, be cooled to
Room temperature, according to 10%(v/v) inoculum concentration kind composite bacteria agent capable B, ferment 36 hours in condition that temperature is 28-30 DEG C, fermented
Liquid B；
Fermentation liquid A, fermentation liquid B and potassium humate are mixed by step 7) according to the mass ratio of 3-5:5-9:1-2, stir,
Then lyophilization is prepared as dry powder, to obtain final product.
Preferably,
In described step 5), described wheat stalk powder, step 2) solution, bean cake, distiller grains, conch meal, potassium dihydrogen phosphate and
The mass ratio of glucose is 10-15:80-120:10-15:5-9:5-9:4-7:1-2.
In described step 6), the mass ratio of described wheat stalk powder, Testa Tritici, rapeseed meal and fishbone powder is 7-10:4-7:
2-3:2-3。
Described trichoderma aureoviride is ACCC No.32248；Pseudomonas aeruginosa is CGMCC No.5721；Described candida mycoderma
Bacterium is ATCC No.22023.
Described arthrobacterium is CGMCC No.7779, and described bacillus megaterium is CGMCC No.3770, and described circle is brown solid
Nitrogen bacterium is ATCC4412.
Above-mentioned bacterial strains all can be bought from preservation center or other commercial channel and obtain.
The beneficial effect that the present invention obtains includes the following aspects:
Fermentation wastes is applied all in fertilizer preparation by the present invention, it is to avoid be evaporated waste liquid concentrating and subtracting at dirt
Reason, decreases energy waste, improves utilization rate, and be prepared for fertilizer, kill two birds with one stone；The bacterium that direct hydrolysis of the present invention is discarded
Body protein is as fermentation raw material, it is provided that abundant ammonium chloride and amino acid nitrogen source, can not only be used for fermentable nutriment, also
Can be as Fertilizer application；Bacterial strain compatibility is reasonable, uses different microbial inoculum different fermentations processing mode so that different microbial inoculums are in most preferably
Fermentation condition, it is to avoid part bacterial strain can not be survived in culture fluid prepared by waste water；The present invention is by effectively utilizing nature to deposit
Agricultural waste material, in conjunction with microbial technique so that fertilizer cost is cheap, fertilizer efficiency is more lasting；Potassium humate is as supporting
Material and carrier, it is possible to nutrients release adjusting function is possessed water conservation fertilizer conservation function；Microbial inoculum of the present invention passes through screening and reasonable compatibility,
Making fertilizer possess fixed nitrogen water conservation, increasing crop yield increases income, and is also equipped with certain weeding function, environment friendly and pollution-free.
Detailed description of the invention
For the technological means making the present invention realize, creation characteristic, reach purpose and be easy to understand with effect, below knot
Close specific embodiment, the present invention is expanded on further.
Embodiment 1
Utilizing Chemical Mixed Fertilizer prepared by Threonine Fermentation garbage, it comprises the steps:
1) the Threonine Fermentation garbage produced by biofermentation threonine (utilizes biological fermentation process to prepare threonine, collects Soviet Union
Garbage after propylhomoserin, the material such as including tropina, saccharide and ammonia nitrogen) filter, collect tropina and waste water；
2) being dried by tropina, pulverizer is ground into powder, is subsequently placed in retort, adds the hydrochloric acid of 5mol/L, with
Not having raw material to be as the criterion, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, uses ammonia after reaction terminating
Neutralizing remaining hydrochloric acid, the pH controlling solution is 6.8-7.2；
3) trichoderma aureoviride, Pseudomonas aeruginosa, candidiasis are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, so
After according to 3:2:2 volume ratio mix, obtain composite bacteria agent capable A；Described trichoderma aureoviride is ACCC No.32248；P. aeruginosa
Bacterium is CN103361284A seen from CGMCC No.5721()；Described candidiasis is ATCC No.22023；
4) arthrobacterium, bacillus megaterium, azotobacter chroococcum are cultivated respectively to concentration be 1 × 108The bacterium solution of individual/ml, then
Mix according to the volume ratio of 2:2:1, obtain composite bacteria agent capable B；Described arthrobacterium is that CGMCC No.7779 is (visible
CN103333837A), described bacillus megaterium is CN102021118A seen from CGMCC No.3770(), the brown fixed nitrogen of described circle
Bacterium is ATCC4412；
5) wheat stalk is ground into wheat stalk powder, adds step 2 to) solution in, stir, stand 12 hours, so
After add bean cake, distiller grains, conch meal, potassium dihydrogen phosphate and glucose successively, 500 revs/min are stirred 3 minutes, then with 5 DEG C/
The programming rate of min is heated to 100 DEG C, is then incubated 30min, naturally cools to room temperature, according to 10%(v/v) inoculum concentration connect
Planting composite bacteria agent capable A, control at pH6.8-7.2, temperature is that the condition of 28-30 DEG C is fermented 36 hours, obtains fermentation liquid A；Wherein, little
Wheat Straw powder, step 2) the mass ratio of solution, bean cake, distiller grains, conch meal, potassium dihydrogen phosphate and glucose be 10:80:
10:5:5:4:1；
6) wheat stalk pulverizing is obtained wheat stalk powder, then obtain with Testa Tritici, rapeseed meal and fishbone powder mixing and stirring
To compound, add the waste water of the step 1) accounting for compound twice quality, soak 12 hours, then boil 15min, be cooled to room
Temperature, according to 10%(v/v) inoculum concentration kind composite bacteria agent capable B, ferment 36 hours in condition that temperature is 28-30 DEG C, obtain fermentation liquid
B；Wherein, the mass ratio of wheat stalk powder, Testa Tritici, rapeseed meal and fishbone powder is 7:4:2:2；
7) fermentation liquid A, fermentation liquid B, potassium humate are mixed according to the mass ratio of 3:5:1, stir, then lyophilization system
Standby one-tenth dry powder, to obtain final product.
Embodiment 2
Utilizing Chemical Mixed Fertilizer prepared by Threonine Fermentation garbage, it comprises the steps:
1) the Threonine Fermentation garbage that biofermentation threonine produces is filtered, collect tropina and waste water；
2) being dried by tropina, pulverizer is ground into powder, is subsequently placed in retort, adds the hydrochloric acid of 5mol/L, with
Not having raw material to be as the criterion, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, uses ammonia after reaction terminating
Neutralizing remaining hydrochloric acid, the pH controlling solution is 6.8-7.2；
3) trichoderma aureoviride, Pseudomonas aeruginosa, candidiasis are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, so
After according to 3:2:2 volume ratio mix, obtain composite bacteria agent capable A；Described trichoderma aureoviride is ACCC No.32248；P. aeruginosa
Bacterium is CGMCC No.5721；Described candidiasis is ATCC No.22023；
4) arthrobacterium, bacillus megaterium, azotobacter chroococcum are cultivated respectively to concentration be 1 × 108The bacterium solution of individual/ml, then
Mix according to the volume ratio of 2:2:1, obtain composite bacteria agent capable B；Described arthrobacterium is CGMCC No.7779, described huge spore bar
Bacterium is CGMCC No.3770, and described azotobacter chroococcum is ATCC4412；
5) wheat stalk is ground into wheat stalk powder, adds step 2 to) solution in, stir, stand 12 hours, so
After add bean cake, distiller grains, conch meal, potassium dihydrogen phosphate and glucose successively, 500 revs/min are stirred 3 minutes, then with 5 DEG C/
The programming rate of min is heated to 100 DEG C, is then incubated 30min, naturally cools to room temperature, according to 10%(v/v) inoculum concentration connect
Planting composite bacteria agent capable A, control at pH6.8-7.2, temperature is that the condition of 28-30 DEG C is fermented 36 hours, obtains fermentation liquid A；Wherein, little
Wheat Straw powder, step 2) the mass ratio of solution, bean cake, distiller grains, conch meal, potassium dihydrogen phosphate and glucose be 15:120:
15:9:9:7:2；
6) wheat stalk pulverizing is obtained wheat stalk powder, then obtain with Testa Tritici, rapeseed meal and fishbone powder mixing and stirring
To compound, add the waste water of the step 1) accounting for compound twice quality, soak 12 hours, then boil 15min, be cooled to room
Temperature, according to 10%(v/v) inoculum concentration kind composite bacteria agent capable B, ferment 36 hours in condition that temperature is 28-30 DEG C, obtain fermentation liquid
B；Wherein, the mass ratio of wheat stalk powder, Testa Tritici, rapeseed meal and fishbone powder is 10:7:3:3；
7) fermentation liquid A, fermentation liquid B, potassium humate are mixed according to the mass ratio of 5:9:2, stir, then lyophilization system
Standby one-tenth dry powder, to obtain final product.
Embodiment 3
The field test effect of the Chemical Mixed Fertilizer of the embodiment of the present invention 2 preparation: as a example by Oryza sativa L..
Matched group: Common compound fertilizer (35% carbamide, 35% MAP, 30% potassium chloride)；
Test group: the Chemical Mixed Fertilizer of Common compound fertilizer (35% carbamide, 35% MAP, 30% potassium chloride)+embodiment 2 preparation.
Experimental technique: select experimental plot rice cultivation, the equal 10 mu of ground of experimental plot area that two groups of fertilizer processes respectively.
Matched group: Common compound fertilizer usage amount is 50kg, test group: Common compound fertilizer usage amount is that 40kg+10kg implements
The Chemical Mixed Fertilizer of example 2 preparation；Planting conditions is identical, gathers in the crops Oryza sativa L. simultaneously, measures Oryza sativa L. per mu yield and rate of growth；Examine simultaneously
Survey Oryza sativa L. grain number per spike, mass of 1000 kernel and unfilled gtains number.
Experimental result: see table 1
Table 1
| Group | Unfilled gtains number (grain/fringe) | Grain number per spike (grain/fringe) | Mass of 1000 kernel (g) | Per mu yield (kg) |
| Matched group | 5.3 | 67.8 | 26.63 | 466.2 |
| Test group | 3.4 | 70.1 | 30.97 | 513.4 |
Same treatment conditions, Second Year multiple cropping Semen Tritici aestivi, experimental result: with reference to table 2.
Table 2
| Group | Unfilled gtains number (grain/fringe) | Grain number per spike (grain/fringe) | Mass of 1000 kernel (g) | Per mu yield (kg) |
| Matched group | 5.7 | 66.1 | 24.93 | 451.9 |
| Test group | 3.3 | 70.5 | 31.86 | 523.7 |
Conclusion: compare discovery by table 1-2, test group Oryza sativa L. unfilled gtains number, grain number per spike, mass of 1000 kernel and per mu yield apparently higher than
Matched group, possesses statistical significance；And the effect of high-yield of Second Year is more preferably, illustrate that bacterial manure effect is more longlasting, flora and humic
Acid potassium serves the effect of fixed nitrogen water conservation weight gaining in soil.
Herbicidal effect: test group is compared with matched group, after applying fertilizer 1 month: Herba chenopodii, Herba Portulacae, barnyard grass, Herba Digitariae, Herba Acalyphae etc.
The quantity of weeds significantly reduces, and wherein, Herba Digitariae decreases 68%, and barnyard grass, Herba Acalyphae decrease 75%；Herbicidal effect is good, can replace
Or the use of minimizing herbicide, and environmentally safe.
Present disclosure is not limited to cited by embodiment, and those of ordinary skill in the art are by reading description of the invention
And the conversion of any equivalence that technical solution of the present invention is taked, the claim being the present invention is contained.
Claims (5)
1. utilizing Chemical Mixed Fertilizer prepared by Threonine Fermentation garbage, it comprises the steps:
The Threonine Fermentation garbage that biofermentation threonine is produced by step 1) filters, and collects tropina and waste water；
Step 2) tropina to be dried, pulverizer is ground into powder, is subsequently placed in retort, adds the salt of 5mol/L
Acid, not have raw material to be as the criterion, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, uses after reaction terminating
With remaining hydrochloric acid in ammonia, the pH controlling solution is 6.8-7.2；
Step 3) trichoderma aureoviride, Pseudomonas aeruginosa, candidiasis are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml,
Then according to the volume ratio mixing of 3:2:2, obtain composite bacteria agent capable A；
Step 4) arthrobacterium, bacillus megaterium, azotobacter chroococcum are cultivated respectively to concentration be 1 × 108The bacterium solution of individual/ml, so
After according to 2:2:1 volume ratio mix, obtain composite bacteria agent capable B；
Wheat stalk is ground into wheat stalk powder by step 5), adds step 2 to) solution in, stir, stand 12 little
Time, add bean cake, distiller grains, conch meal, potassium dihydrogen phosphate and glucose the most successively, 500 revs/min are stirred 3 minutes, then with
The programming rate of 5 DEG C/min is heated to 100 DEG C, is then incubated 30min, naturally cools to room temperature, according to 10%(v/v) inoculation
Amount inoculating compound bacterium agent A, controls at pH6.8-7.2, and temperature is that the condition of 28-30 DEG C is fermented 36 hours, obtains fermentation liquid A；
Wheat stalk is pulverized and is obtained wheat stalk powder by step 6), then equal with Testa Tritici, rapeseed meal and fishbone powder mix and blend
The even compound that obtains, adds the waste water of the step 1) accounting for compound twice quality, soaks 12 hours, then boil 15min, be cooled to
Room temperature, according to 10%(v/v) inoculum concentration inoculating compound bacterium agent B, ferment 36 hours in condition that temperature is 28-30 DEG C, sent out
Ferment liquid B；
Fermentation liquid A, fermentation liquid B, potassium humate are mixed by step 7) according to the mass ratio of 3-5:5-9:1-2, stir, then
Lyophilization is prepared as dry powder, to obtain final product.
Chemical Mixed Fertilizer the most according to claim 1, it is characterised in that in described step 5), described wheat stalk powder, step 2)
The mass ratio of solution, bean cake, distiller grains, conch meal, potassium dihydrogen phosphate and glucose be 10-15:80-120:10-15:5-9:
5-9:4-7:1-2。
Chemical Mixed Fertilizer the most according to claim 1, it is characterised in that in described step 6), described wheat stalk powder, Testa Tritici,
The mass ratio of rapeseed meal and fishbone powder is 7-10:4-7:2-3:2-3.
Chemical Mixed Fertilizer the most according to claim 1, it is characterised in that described trichoderma aureoviride is ACCC No.32248；Aerugo is false
Zymomonas mobilis is CGMCC No.5721；Described candidiasis is ATCC No.22023.
Chemical Mixed Fertilizer the most according to claim 1, it is characterised in that described arthrobacterium is CGMCC No.7779, described huge
Bacillus cereus is CGMCC No.3770, and described azotobacter chroococcum is ATCC4412.
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| CN106116773A (en) * | 2016-07-01 | 2016-11-16 | 内蒙古阜丰生物科技有限公司 | Amino acid fermentation is utilized to discard the technique that thalline prepares Liquid organic fertilizer |
| CN106146088A (en) * | 2016-06-30 | 2016-11-23 | 内蒙古阜丰生物科技有限公司 | Utilize the method that amino acids production waste liquid prepares fertilizer |
| CN106187423A (en) * | 2016-06-30 | 2016-12-07 | 内蒙古阜丰生物科技有限公司 | A kind of bio-bacterial manure comprising amino acid fermentation garbage |
| CN110937940A (en) * | 2019-12-21 | 2020-03-31 | 赵兰坤 | Method for producing bio-organic fertilizer by using threonine waste liquid and corn leftovers |
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| CN106116773A (en) * | 2016-07-01 | 2016-11-16 | 内蒙古阜丰生物科技有限公司 | Amino acid fermentation is utilized to discard the technique that thalline prepares Liquid organic fertilizer |
| CN106116773B (en) * | 2016-07-01 | 2019-05-24 | 内蒙古阜丰生物科技有限公司 | The technique for discarding thallus preparation Liquid organic fertilizer using amino acid fermentation |
| CN110937940A (en) * | 2019-12-21 | 2020-03-31 | 赵兰坤 | Method for producing bio-organic fertilizer by using threonine waste liquid and corn leftovers |
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