CN105859363B - The preparation method of compound fertilizer comprising amino acid fermentation waste liquid - Google Patents
The preparation method of compound fertilizer comprising amino acid fermentation waste liquid Download PDFInfo
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- CN105859363B CN105859363B CN201610396386.1A CN201610396386A CN105859363B CN 105859363 B CN105859363 B CN 105859363B CN 201610396386 A CN201610396386 A CN 201610396386A CN 105859363 B CN105859363 B CN 105859363B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention belongs to waste material preparation fields, disclose the preparation method of the compound fertilizer comprising amino acid fermentation waste liquid, it includes the following steps: that step 1) filters waste liquid, step 2 hydrolysis, step 3) prepares composite bacteria agent A, and step 4) prepares composite bacteria agent B, and step 5) prepares fermentation liquid A, step 6) prepares fermentation liquid B, step 7) freeze-drying.The advantages that fertilizer prepared by the present invention has fertilizer efficiency lasting, has certain weeding function, and environment friendly and pollution-free.
Description
Technical field
The invention belongs to fertilizer preparation fields, and in particular to the preparation method of the compound fertilizer comprising amino acid fermentation waste liquid.
Background technique
Currently, biofermentation enterprise generates a large amount of waste liquid during fermenting and producing various amino acid, due to the ammonia that ferments
There is COD, SO in base acid waste water4 2-And NH3The features such as N content is high and acid strong, in addition, also containing a large amount of thallus in waste liquid
Albumen, reduced sugar, ammonia nitrogen if any discharge not only causes serious environmental pollution, but also waste precious resources.If will
Waste liquid is handled, and investment is huge, does not also meet recycling economy development requirement.Fermenting and producing amino acid waste liquid is comprehensively utilized
Come, for biofermentation amino acid Sustainable Development of Enterprises, there is important strategic importance.Biofermentation production amino acid is given up
The problem of liquid is processed into organic-inorganic complex fertilizer, that is, the processing for solving waste water is difficult, pollutes environment, and bring to enterprise aobvious
The economic benefit of work.The patented technology of applicant early period is " a kind of to prepare organic and inorganic compound using biofermentation amino acid wastewater
Waste liquid is applied in fertilizer preparation by the technique of fertilizer ", solves the problems, such as liquid waste processing, and be prepared for fertilizer, but the technology
There are still certain defects, for example, mycoprotein can not be efficiently used, waste energy etc. using concentration and evaporation step.
Currently, after fermentation byproduct mycoprotein passes through flocculation sedimentation plate-frame filtering, directly as cheap forage protein pin
It sells, added value of product is lower；Waste water is expelled directly out by simple process；But the above method there is no by various waste materials respectively into
Row processing, does not utilize added value to greatest extent.How fermented waste fluid is effectively utilized, it is additional to improve industry to greatest extent
Value, is the direction that enterprise is pursued always.
It is Main Stage that the development of China's fertilizer and application history, which are from farmyard manure to inorganic fertilizer is used, because of farmyard manure dirt
Dye source is more, and freight volume is big, and operationally time-consuming and laborious and effect is not particularly evident；And inorganic fertilizer composition is single, utilization rate
It is lower, and often will cause soil hardening and with soil erosion polluted source, influence ecological environment.Currently, market is adopted more
With the inorganic composite fertilizer etc. of Nitrogen-and Phosphorus-containing potassium, the fertilizer efficiency of the compound fertilizer is comprehensive, not lasting but there is also fertilizer efficiency, is easy loss etc. and lacks
It falls into；Importantly, the price of compound fertilizer is higher at present, biggish burden is brought to peasant, how to reduce fertilizer cost, improved
Farmers' income is the agricultural modernization technical issues that need to address.
Summary of the invention
The present invention is to solve biofermentation enterprise to produce the waste liquid generated during various amino acid and compound fertilizer fertilizer efficiency
The problems such as not lasting enough, the object of the present invention is to provide the preparation method of the compound fertilizer comprising amino acid fermentation waste liquid, preparations
Fertilizer have fertilizer efficiency lasting, have certain weeding function, and it is environment friendly and pollution-free the advantages that.
The present invention completes by following technical solution:
The preparation method of compound fertilizer comprising amino acid fermentation waste liquid comprising following steps:
Step 1) filters waste liquid, step 2 hydrolysis, and step 3) prepares composite bacteria agent A, and step 4) prepares composite bacteria agent B, step
Rapid 5) to prepare fermentation liquid A, step 6) prepares fermentation liquid B, step 7) freeze-drying.
Specifically, the preparation method includes the following steps:
1) the amino acid fermentation waste liquid for generating biofermentation amino acid filters, and collects mycoprotein and waste water；
2) mycoprotein is dried, pulverizer is pulverized into powder, and is subsequently placed in reactor tank, and the salt of 5mol/L is added
Acid is subject to and does not cross raw material, and stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, uses after reaction terminating
With remaining hydrochloric acid in ammonium hydroxide, the pH for controlling solution is 6.8-7.2；
3) cultivating trichoderma aureoviride, pseudomonas aeruginosa, candidiasis to concentration respectively is 1 × 107The bacterium of a/ml
Then liquid is mixed according to the volume ratio of 2:1:1 to get composite bacteria agent A；
4) cultivating arthrobacterium, bacillus megaterium, bacillus subtilis, azotobacter chroococcum to concentration respectively is 1 × 108
Then the bacterium solution of a/ml is mixed according to the volume ratio of 3:2:2:1 to get composite bacteria agent B；
5) broomcorn straw is ground into broomcorn straw powder, be added in the solution of step 2, stirred evenly, it is small to stand 12
When, dregs of beans, rice husk, conch meal, potassium dihydrogen phosphate and glucose are then successively added, 500 revs/min are stirred 3 minutes, then with
The heating rate of 5 DEG C/min is heated to 100 DEG C, then keeps the temperature 30min, cooled to room temperature, according to 10%(v/v) inoculation
Inoculating compound bacterium agent A is measured, control is fermented 36 hours in pH6.8-7.2, the condition that temperature is 28-30 DEG C, obtains fermentation liquid A；Its
In, broomcorn straw powder, the solution of step 2, dregs of beans, rice husk, conch meal, potassium dihydrogen phosphate and glucose mass ratio be 16-
25:120-170:12-16:7-12:7-9:7-9:2-3；
6) broomcorn straw is crushed to obtain broomcorn straw powder, is then mixed with wheat bran, manioc waste and fishbone dust equal
It is even to obtain mixture, the waste water for accounting for the step 1) of twice of mass of mixture is added, impregnates 12 hours, then boil 15min, is cooled to
Room temperature, according to 8%(v/v) inoculum concentration kind composite bacteria agent B, temperature be 28-30 DEG C condition ferment 36 hours, fermented
Liquid B；Wherein, broomcorn straw powder, wheat bran, manioc waste and the mass ratio of fishbone dust are 5-9:3-5:3-5:2-3；
7) fermentation liquid A, fermentation liquid B and potassium humate are mixed according to the mass ratio of 3-5:5-9:1-2, are stirred evenly,
Then freeze-drying be prepared into dry powder to get.
Preferably, the trichoderma aureoviride is ACCC No.32248；Pseudomonas aeruginosa is CGMCC No.5721；The vacation
Silk saccharomycete is ATCC No.22023.
Preferably, the arthrobacterium is CGMCC No.7779, and the bacillus megaterium is CGMCC No.3770, described
Bacillus subtilis is CGMCC No.0954, and the azotobacter chroococcum is ATCC4412.
Above-mentioned bacterial strains can be obtained from collection or the purchase of other commercial channel.
The beneficial effect that the present invention obtains includes the following aspects:
Fermented waste fluid is applied all to avoid in fertilizer preparation and be evaporated concentration to waste liquid and subtract dirt by the present invention
Processing, reduces energy waste, improves utilization rate, and be prepared for fertilizer, kill two birds with one stone；Direct hydrolysis fermentation of the present invention is useless
Mycoprotein in liquid provides ammonium chloride and amino acid nitrogen source abundant as fermentation raw material, can not only be used for microorganism hair
Ferment nutriment, also can be used as Fertilizer application；Bacterial strain compatibility is reasonable, using different microbial inoculum different fermentations processing modes, so that different bacterium
Agent is in optimal fermentation condition, avoids part bacterial strain that from cannot surviving in culture solution prepared by waste water；The present invention passes through effective
Using the agricultural waste material of naturally occurring, in conjunction with microbial technique, so that fertilizer cost is cheap, fertilizer efficiency is more lasting；Corruption is planted
Sour potassium can have retain water and nutrients function as nutriment and carrier to nutrients release adjusting function；Microbial inoculum of the present invention is by screening
And reasonable compatibility, so that fertilizer has fixed nitrogen water conservation, increasing crop yield is increased income, and is also equipped with certain weeding function, environmental protection is without dirt
Dye.
Specific embodiment
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie below
Specific embodiment is closed, the present invention is further explained.
Embodiment 1
The preparation method of compound fertilizer comprising amino acid fermentation waste liquid comprising following steps:
1) the amino acid fermentation waste liquid that biofermentation amino acid generates (is prepared amino acid using biological fermentation process, collected
The substances such as the remaining waste after amino acid, including mycoprotein, carbohydrate and ammonia nitrogen) filtering, collect mycoprotein and waste water；
2) mycoprotein is dried, pulverizer is pulverized into powder, and is subsequently placed in reactor tank, and the salt of 5mol/L is added
Acid is subject to and does not cross raw material, and stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, uses after reaction terminating
With remaining hydrochloric acid in ammonium hydroxide, the pH for controlling solution is 6.8-7.2；
3) cultivating trichoderma aureoviride, pseudomonas aeruginosa, candidiasis to concentration respectively is 1 × 107The bacterium of a/ml
Then liquid is mixed according to the volume ratio of 2:1:1 to get composite bacteria agent A；The trichoderma aureoviride is ACCC No.32248；Verdigris is false
Monad is the visible CN103361284A of CGMCC No.5721()；The candidiasis is ATCC No.22023；
4) cultivating arthrobacterium, bacillus megaterium, bacillus subtilis, azotobacter chroococcum to concentration respectively is 1 × 108
Then the bacterium solution of a/ml is mixed according to the volume ratio of 3:2:2:1 to get composite bacteria agent B；The arthrobacterium is CGMCC
No.7779 (CN103333837A), the bacillus megaterium are CGMCC No.3770(CN102021118A), the withered grass
Bacillus is CGMCC No.0954 (CN1554744A), and the azotobacter chroococcum is ATCC4412；
5) broomcorn straw is ground into broomcorn straw powder, be added in the solution of step 2, stirred evenly, it is small to stand 12
When, dregs of beans, rice husk, conch meal, potassium dihydrogen phosphate and glucose are then successively added, 500 revs/min are stirred 3 minutes, then with
The heating rate of 5 DEG C/min is heated to 100 DEG C, then keeps the temperature 30min, cooled to room temperature, according to 10%(v/v) inoculation
Inoculating compound bacterium agent A is measured, control is fermented 36 hours in pH6.8-7.2, the condition that temperature is 28-30 DEG C, obtains fermentation liquid A；Its
In, broomcorn straw powder, the solution of step 2, dregs of beans, rice husk, conch meal, potassium dihydrogen phosphate and glucose mass ratio be 25:
170:16:12:9:9:3；
6) broomcorn straw is crushed to obtain broomcorn straw powder, is then mixed with wheat bran, manioc waste and fishbone dust equal
It is even to obtain mixture, the waste water for accounting for the step 1) of twice of mass of mixture is added, impregnates 12 hours, then boil 15min, is cooled to
Room temperature, according to 8%(v/v) inoculum concentration kind composite bacteria agent B, temperature be 28-30 DEG C condition ferment 36 hours, fermented
Liquid B；Wherein, broomcorn straw powder, wheat bran, manioc waste and the mass ratio of fishbone dust are 9:5:5:3；
7) fermentation liquid A, fermentation liquid B and potassium humate are mixed according to the mass ratio of 5:9:2, is stirred evenly, it is then cold
Be lyophilized it is dry be prepared into dry powder to get.
Embodiment 2
The preparation method of compound fertilizer comprising amino acid fermentation waste liquid comprising following steps:
1) the amino acid fermentation waste liquid for generating biofermentation amino acid filters, and collects mycoprotein and waste water；
2) mycoprotein is dried, pulverizer is pulverized into powder, and is subsequently placed in reactor tank, and the salt of 5mol/L is added
Acid is subject to and does not cross raw material, and stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, uses after reaction terminating
With remaining hydrochloric acid in ammonium hydroxide, the pH for controlling solution is 6.8-7.2；
3) cultivating trichoderma aureoviride, pseudomonas aeruginosa, candidiasis to concentration respectively is 1 × 107The bacterium of a/ml
Then liquid is mixed according to the volume ratio of 2:1:1 to get composite bacteria agent A；The trichoderma aureoviride is ACCC No.32248；Verdigris is false
Monad is CGMCC No.5721；The candidiasis is ATCC No.22023；
4) cultivating arthrobacterium, bacillus megaterium, bacillus subtilis, azotobacter chroococcum to concentration respectively is 1 × 108
Then the bacterium solution of a/ml is mixed according to the volume ratio of 3:2:2:1 to get composite bacteria agent B；The arthrobacterium is CGMCC
No.7779, the bacillus megaterium are CGMCC No.3770, and the bacillus subtilis is CGMCC No.0954, described
Azotobacter chroococcum is ATCC4412；
5) broomcorn straw is ground into broomcorn straw powder, be added in the solution of step 2, stirred evenly, it is small to stand 12
When, dregs of beans, rice husk, conch meal, potassium dihydrogen phosphate and glucose are then successively added, 500 revs/min are stirred 3 minutes, then with
The heating rate of 5 DEG C/min is heated to 100 DEG C, then keeps the temperature 30min, cooled to room temperature, according to 10%(v/v) inoculation
Inoculating compound bacterium agent A is measured, control is fermented 36 hours in pH6.8-7.2, the condition that temperature is 28-30 DEG C, obtains fermentation liquid A；Its
In, broomcorn straw powder, the solution of step 2, dregs of beans, rice husk, conch meal, potassium dihydrogen phosphate and glucose mass ratio be 16:
120:12:7:7:7:2；
6) broomcorn straw is crushed to obtain broomcorn straw powder, is then mixed with wheat bran, manioc waste and fishbone dust equal
It is even to obtain mixture, the waste water for accounting for the step 1) of twice of mass of mixture is added, impregnates 12 hours, then boil 15min, is cooled to
Room temperature, according to 8%(v/v) inoculum concentration kind composite bacteria agent B, temperature be 28-30 DEG C condition ferment 36 hours, fermented
Liquid B；Wherein, broomcorn straw powder, wheat bran, manioc waste and the mass ratio of fishbone dust are 5:3:3:2；
7) fermentation liquid A, fermentation liquid B and potassium humate are mixed according to the mass ratio of 3:5:1, is stirred evenly, it is then cold
Be lyophilized it is dry be prepared into dry powder to get.
Embodiment 3
The field test effect of compound fertilizer prepared by the embodiment of the present invention 1: by taking wheat as an example.
Control group: Common compound fertilizer (N-P-K content 15: 15: 15)；
Test group: the compound fertilizer of Common compound fertilizer (N-P-K content 15: 15: 15)+embodiment 1 preparation.
Experimental method: experimental plot Winter Wheat Planted, the equal 10 mu of ground of the experimental plot area that two groups of fertilizer are handled respectively are selected.
Control group: Common compound fertilizer usage amount is 40kg, and test group: Common compound fertilizer usage amount is 30kg+10kg implementation
Compound fertilizer prepared by example 1；Other planting conditions are identical；Wheat is harvested simultaneously, measures wheat per mu yield and rate of growth；Together
When detect wheat grains per spike, mass of 1000 kernel.Experimental result: referring to table 1.
Table 1
| Group | Grain number per spike | Mass of 1000 kernel (g) | Per mu yield (kg) |
| Control group | 27.5 | 46.4 | 365 |
| Test group | 31.9 | 47.8 | 393 |
Same treatment conditions, second year multiple cropping wheat, experimental result: referring to table 2.
Table 2
| Group | Grain number per spike | Mass of 1000 kernel (g) | Per mu yield (kg) |
| Control group | 26.9 | 46.3 | 359 |
| Test group | 32.7 | 48.3 | 403 |
Conclusion: by table 1-2, it was found that, test group wheat grains per spike, mass of 1000 kernel and per mu yield are apparently higher than control
Group has statistical significance；And the effect of high-yield of second year is more preferably, illustrates that bacterial manure effect is more longlasting, flora and potassium humate
The effect of fixed nitrogen water conservation getting fat is played in soil.
Herbicidal effect: test group compared with the control group, fertilising 1 month after: Chenopodiaceae, purslane, barnyard grass, herba digitariae, iron amaranth grass etc.
The quantity of weeds significantly reduces, wherein herba digitariae reduces 65%, and barnyard grass reduces 73%；Herbicidal effect is good, can replace or reduce to remove
The use of careless agent, and no pollution to the environment.
The contents of the present invention are not limited to cited by embodiment, and those of ordinary skill in the art are by reading description of the invention
And to any equivalent transformation that technical solution of the present invention is taken, all are covered by the claims of the invention.
Claims (1)
1. the preparation method of the compound fertilizer comprising amino acid fermentation waste liquid comprising following steps:
1) the amino acid fermentation waste liquid for generating biofermentation amino acid filters, and collects mycoprotein and waste water；
2) mycoprotein to be dried, pulverizer is pulverized into powder, and is subsequently placed in reactor tank, the hydrochloric acid of 5mol/L is added, with
It does not cross subject to raw material, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, uses ammonium hydroxide after reaction terminating
Remaining hydrochloric acid is neutralized, the pH for controlling solution is 6.8-7.2；
3) cultivating trichoderma aureoviride, pseudomonas aeruginosa, candidiasis to concentration respectively is 1 × 107The bacterium solution of a/ml, then
It mixes according to the volume ratio of 2:1:1 to get composite bacteria agent A；
4) cultivating arthrobacterium, bacillus megaterium, bacillus subtilis, azotobacter chroococcum to concentration respectively is 1 × 108A/ml
Bacterium solution, then according to 3:2:2:1 volume ratio mix to get composite bacteria agent B；
5) broomcorn straw is ground into broomcorn straw powder, be added in the solution of step 2, stirred evenly, stand 12 hours, so
Successively add dregs of beans, rice husk, conch meal, potassium dihydrogen phosphate and glucose afterwards, 500 revs/min are stirred 3 minutes, then with 5 DEG C/
The heating rate of min is heated to 100 DEG C, then keeps the temperature 30min, and cooled to room temperature is compound according to 10% inoculum concentration inoculation
Microbial inoculum A, control are fermented 36 hours in pH6.8-7.2, the condition that temperature is 28-30 DEG C, obtain fermentation liquid A；Wherein, broomcorn straw
Powder, the solution of step 2, dregs of beans, rice husk, conch meal, potassium dihydrogen phosphate and glucose mass ratio be 16-25:120-170:
12-16:7-12:7-9:7-9:2-3；
6) broomcorn straw is crushed to obtain broomcorn straw powder, is then mixed evenly with wheat bran, manioc waste and fishbone dust
To mixture, the waste water for accounting for the step 1) of twice of mass of mixture is added, impregnates 12 hours, then boil 15min, is cooled to room
Temperature ferments 36 hours in the condition that temperature is 28-30 DEG C according to 8% inoculum concentration inoculating compound bacterium agent B, obtains fermentation liquid B；Its
In, broomcorn straw powder, wheat bran, manioc waste and the mass ratio of fishbone dust are 5-9:3-5:3-5:2-3；
7) fermentation liquid A, fermentation liquid B and potassium humate are mixed according to the mass ratio of 3-5:5-9:1-2, is stirred evenly, then
Freeze-drying be prepared into dry powder to get；
The trichoderma aureoviride is ACCC No.32248；Pseudomonas aeruginosa is CGMCC No.5721；The candidiasis is
ATCC No.22023；
The arthrobacterium is CGMCC No.7779, and the bacillus megaterium is CGMCC No.3770, the bacillus subtilis
Bacterium is CGMCC No.0954, and the azotobacter chroococcum is ATCC4412.
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| CN105948972A (en) * | 2016-04-25 | 2016-09-21 | 韩德辉 | Amino acid fertilizer and preparation method and application thereof |
| CN105831163A (en) * | 2016-05-17 | 2016-08-10 | 高秀兰 | Compound weed killer special for crops and preparing method thereof |
| CN106187423B (en) * | 2016-06-30 | 2019-05-24 | 内蒙古阜丰生物科技有限公司 | A kind of bio-bacterial manure comprising amino acid fermentation waste |
| CN106146088B (en) * | 2016-06-30 | 2019-05-24 | 内蒙古阜丰生物科技有限公司 | The method for preparing fertilizer using amino acids production waste liquid |
| CN109970495A (en) * | 2017-12-28 | 2019-07-05 | 许传高 | A kind of technique preparing organic fertilizer using amino acid fermentation tail washings |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104261947A (en) * | 2014-10-07 | 2015-01-07 | 内蒙古阜丰生物科技有限公司 | Fertilizer prepared by utilizing threonine fermented wastes |
| CN104725147A (en) * | 2015-02-12 | 2015-06-24 | 新疆阜丰生物科技有限公司 | Process for preparing organic-inorganic compound fertilizer from bio-fermented amino acid wastewater |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104261947A (en) * | 2014-10-07 | 2015-01-07 | 内蒙古阜丰生物科技有限公司 | Fertilizer prepared by utilizing threonine fermented wastes |
| CN104725147A (en) * | 2015-02-12 | 2015-06-24 | 新疆阜丰生物科技有限公司 | Process for preparing organic-inorganic compound fertilizer from bio-fermented amino acid wastewater |
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