CN106754476A - Water-soluble vitamins microbial inoculum and its preparation method and application - Google Patents
Water-soluble vitamins microbial inoculum and its preparation method and application Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The invention discloses a kind of water-soluble vitamins microbial inoculum and its preparation method and application.The present invention discloses a kind of water-soluble vitamins microbial inoculum first, including：Bacillus subtilis microbial agent, bacillus licheniformis agent, bacillus amyloliquefaciens microbial inoculum, fulvic acid, amino acid, acetic acid and molasses.On the basis of above-mentioned water-soluble vitamins microbial inoculum, further addition lactobacillus plantarum microbial inoculum and bacillus laterosporus microbial inoculum, with preferably improvement soil microenvironment, improves crop yield and the effect of quality to the present invention.The present invention further discloses a kind of method for preparing the water-soluble vitamins microbial inoculum, including：Each composition is weighed according to the weight portion, adds water to be well mixed, anaerobic fermentation is obtained final product.Each microbial inoculum collocation is reasonable in water-soluble vitamins microbial inoculum of the present invention, there is obvious synergistic function between microbial inoculum, can effectively adjust soil texture, alleviates continuous cropping obstacle, improves soil water-reataining fertilizer-keeping ability, reaches raising crop yield and quality.
Description
Technical field
The present invention relates to a kind of water-soluble vitamins microbial inoculum, further relate to the water-soluble vitamins microbial inoculum preparation method and
Its application in facility plastic greenhouse soil, belongs to preparation and the application field of microbial bacterial agent.
Background technology
After facility plastic greenhouse due to excessively applying chemical fertilizer, agricultural chemicals for years, soil hardening is degenerated, and continuous cropping obstacle is sent out often
It is raw, cause crop disease and insect multiple.Microbial bacterial agent is poured onto in soil with water, is bred by beneficial microbe, can be promoted
Nutrient Transformation in organic materials, supplements and the balanced available nutrient of soil, promotes the decomposition of organic matter, and conversion forms having for high-quality
Machine fertilizer, reduces the soil weight, promotes the formation of soil granular structure, significantly improves the microecosystem of soil, enhancing soil
Earth gas permeability, improves soil water-reataining fertilizer-keeping ability, is finally reached the purpose fertilized the soil and increase soil fertility.
Therefore, a kind of new fertilizer in facility plastic greenhouse, persticide residue of being effectively improved is developed excessive, continuous cropping obstacle
Seriously, the water-soluble vitamins microbial inoculum of improved soil ecological environment, will be of great significance and value.
The content of the invention
Present invention technical problem to be solved first is to provide a kind of water-soluble vitamins microbial inoculum, the water-soluble vitamins
Microbial inoculum can significantly improve that fertilizer in facility plastic greenhouse soil, persticide residue are excessive, and the serious problem of continuous cropping obstacle can be improved
Ecological environment of soil, and improve the effect is significant of crop yield or quality；
Another technical problem to be solved by this invention is to provide a kind of preparation side of the water-soluble vitamins microbial inoculum
Method.
In order to solve the above technical problems, the technical solution used in the present invention is：
The present invention discloses a kind of water-soluble vitamins microbial inoculum, including following composition first：Bacillus subtilis microbial agent,
Bacillus licheniformis agent, bacillus amyloliquefaciens microbial inoculum, fulvic acid, amino acid, acetic acid and molasses.
The weight portion of each composition is：Bacillus subtilis microbial agent 1-2 parts, bacillus licheniformis agent 1-2 parts, solution starch bud
1-2 parts, fulvic acid 60-70 parts, amino acid 30-40 parts, acetic acid 5-10 parts, molasses 5-10 parts of spore bacillus microbial inoculum.Preferably, respectively into
Point weight portion be：2 parts of bacillus subtilis microbial agent, 2 parts of bacillus licheniformis agent, 2 parts of bacillus amyloliquefaciens microbial inoculum, Huang
Rotten 60 parts of acid, 40 parts of amino acid, 5 parts of acetic acid, 10 parts of molasses.
Auxiliary material fulvic acid of the present invention is solid, and amino acid, acetic acid and molasses are liquid.
Molasses are the byproduct of sugar industry, wherein mainly containing a large amount of fermentable sugars (mainly sucrose).
Following composition is further added in water-soluble vitamins microbial inoculum of the present invention, with preferably improvement soil
Micro-ecological environment, increase soil fertility, improve the effect of crop yield or quality：Lactobacillus plantarum microbial inoculum and bacillus laterosporus
Microbial inoculum；The weight portion of each composition is：Lactobacillus plantarum microbial inoculum 1-2 parts, 1-5 parts of bacillus laterosporus microbial inoculum；Preferably, each composition
Weight portion be：2 parts of lactobacillus plantarum microbial inoculum, 3 parts of bacillus laterosporus microbial inoculum.
Microbial inoculum compatibility Optimal Experimental shows that the present invention conciliates in bacillus subtilis microbial agent, bacillus licheniformis agent forms sediment
Lactobacillus plantarum microbial inoculum and bacillusmusilaginosiengineering microbial inoculum, Lactobacillus plantarum are further added on the basis of afnyloliquefaciens microbial inoculum
The different microbial inoculum combination such as microbial inoculum and colloid bacillus cereus microbial inoculum, lactobacillus plantarum microbial inoculum and bacillus laterosporus microbial inoculum, is carried out
The application effect experiment of tomato in plastic greenhouse production, as a result adding prepared by lactobacillus plantarum microbial inoculum and bacillus laterosporus microbial inoculum
Microbial bacterial agent, the effect for improving tomato yield and fruit weight is the most notable, tomato production promoting rate up to 36.1%, than adding other
The treatment of microbial inoculum combination improves 19.3-34.3 percentage points；Tamato fruit rate of body weight gain is combined up to 15.1% than adding other microbial inoculums
Improve 10.2-14.9 percentage points.Result above proves, bacillus subtilis microbial agent, bacillus licheniformis agent, solution starch bud
There is obvious synergistic function between spore bacillus microbial inoculum and lactobacillus plantarum microbial inoculum, bacillus laterosporus microbial inoculum.
Bacillus subtilis microbial agent of the present invention, bacillus licheniformis agent, bacillus amyloliquefaciens microbial inoculum, plant breast
Bacillus microbial inoculum and bacillus laterosporus microbial inoculum are powdery microbial inoculum, and living bacteria count is >=1.0 × 1010Cfu/ grams, can be by normal
Rule bacterial preparation process prepares microbial inoculum, can be also commercially available from market.
The present invention is to bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens, Lactobacillus plantarum and side spore gemma
The specific bacterial strain of bacillus does not have any specifically limited, and any bacterial strain obtained by commercial sources or other approach can be suitably used for
The present invention.
All microorganism fungus kinds used in the present invention (are purchased from culture presevation administrative center of the Chinese Academy of Agricultural Sciences, China
Microbiological Culture Collection administration committee common micro-organisms center, living bacteria count is more than or equal to 200,000,000/ml, and miscellaneous bacteria rate is less than
Or equal to 10%) can be commercially available from market with other raw materials, its specification meets national sector standard.
Water-soluble vitamins microbial inoculum of the present invention is rich in soluble organic nutrient, and pH value is in subacidity, it is adaptable to secondary
Salinization soil, hardened degenerated soil, continuous cropping obstacle nutrient imbalance soil, soil texture is adjusted by microorganism, forms advantage
Flora, promotes the decomposition of organic matter, and effectively conversion forms the organic fertilizer of high-quality, reduces the soil weight, promotes soil granular knot
The formation of structure, significantly improves the microecosystem of soil, and balanced soil nutrient suppresses miscellaneous bacteria and multiplies, and strengthens the gas permeability of soil,
Effectively alleviate continuous cropping obstacle, improve soil water-reataining fertilizer-keeping ability, reach the purpose fertilized the soil and increase soil fertility.
The present invention further discloses a kind of preparation method of the water-soluble vitamins microbial inoculum, comprise the following steps：Press
Each composition is weighed according to the weight portion, adds water to be well mixed, fermentation is obtained final product.Wherein, the weight portion of the water is 65-100
Part, preferably 67 parts；The condition of the fermentation be 28-35 DEG C anaerobic fermentation 60-80 hours；Preferably 30 DEG C anaerobic fermentations 70 are small
When.
Water-soluble vitamins microbial inoculum of the present invention can be applied in facility plastic greenhouse soil, big particularly for improving facility
The soil fertility or improved soil ecological environment of canopy, and improve crop yield or the quality of facility plastic greenhouse.Wherein, the crop
Preferably tomato.
Using microbial bacterial agent of the present invention in tomato in plastic greenhouse production, tomato production promoting rate reaches 33.7%, average single
Fruit rate of body weight gain can significantly improve the fruit quality of tomato up to 13.9%, and especially Vc contents improve 16.4%.Prove,
In the case of greenhouse fertilizer abundance, microbial bacterial agent of the present invention can improve micro-ecological environment around root system, promote root system nutrient
Absorb, improve the utilization rate of soil and fertilizer, reduce fertilizer loss, tomato yield and single fruit weight is significantly improved.
Water-soluble vitamins microbial inoculum method of the present invention and consumption：Microbial bacterial agent mu consumption 1-2 liters/mu, fill with water
Irrigate, the serious multiplicable consumption of soil hardening degeneration.
Technical solution of the present invention has the advantages that compared with prior art：
Each microbial inoculum compatibility of water-soluble vitamins microbial inoculum of the present invention is reasonable, bacillus subtilis microbial agent, lichens gemma
There is obvious association between bacillus microbial inoculum, bacillus amyloliquefaciens microbial inoculum and lactobacillus plantarum microbial inoculum, bacillus laterosporus microbial inoculum
Same synergistic effect, can effectively improve soil microenvironment, and balanced soil nutrient is effectively alleviated continuous cropping obstacle, suppressed miscellaneous
Bacterium multiplies, and strengthens the gas permeability of soil, improves soil water-reataining fertilizer-keeping ability, reaches and effectively improves agricultural output and improve agricultural product
The effect of quality.
Specific embodiment
Further describe the present invention with reference to specific embodiment, advantages of the present invention and feature will be with description and
It is apparent.It should be understood that the embodiment is only exemplary, any limitation is not constituted to the scope of the present invention.This area
Technical staff should be understood that without departing from the spirit and scope of the invention can to the details of technical solution of the present invention and
Form is modified or is replaced, but these modifications or replacement each fall within protection scope of the present invention.
1st, strain
Bacillus subtilis strain (Bacillus subtilis), purchased from China Committee for Culture Collection of Microorganisms
Common micro-organisms center, bacterium numbering AS 1.11086；Bacillus licheniformis agent (Bacillus licheniformis) is purchased
From in Chinese agriculture Microbiological Culture Collection administrative center, bacterium numbering AS 1.10314；Bacillus amyloliquefaciens strain
(Bacillus amyloliquefaciens) is purchased from China Committee for Culture Collection of Microorganisms's common micro-organisms center, bacterium
Plant numbering AS1.10901；Lactobacillus plantarum (Lactobacillus plantarum) strain is purchased from Chinese microorganism strain preservation
Administration committee's common micro-organisms center, bacterium numbering 1.3921；Bacillus laterosporus microbial inoculum, purchased from the green section's biotechnology in Jiangsu
Co., Ltd, effective viable bacteria content >=1.0 × 1010cfu/g。
Lactobacillus acidophilus (Lactobacillus acidophilus), purchased from China General Microbiological culture presevation pipe
Reason center, bacterium numbering 1.1854；Bacillus coagulans, purchased from the common micro- life of China Committee for Culture Collection of Microorganisms
Thing center, bacterium numbering 1.3220；Bacillusmusilaginosiengineering microbial inoculum, bacterium numbering CICC No.21699 are micro- purchased from Chinese industrial
Biological inoculum preservation administrative center；Colloid bacillus cereus microbial inoculum, bacterium numbering ACCC10013；Bacillus thuringiensis, strain is compiled
Number ACCC11075, purchased from Chinese agriculture Microbiological Culture Collection administrative center；Bacillus megaterium microbial inoculum, purchased from Yangzhou sea
Sincere Bioisystech Co., Ltd, viable bacteria content >=10,000,000,000/gram.
The preparation of the bacillus subtilis microbial agent of Preparative Example 1
Bacillus subtilis solid fungicide can be prepared conventionally, for example, the withered grass bud that will be commercially available
Spore bacillus strain is inoculated into culture in seed culture and obtains inoculum；Inoculum is inoculated into the fermentation in fermentation tank
Fermented in culture medium, filler and the adsorbent crushed are added after the completion of fermentation, obtain bacillus subtilis solid fungicide；Specifically
, refer to following methods and prepare：
(1) Bacillus subtilis strain (Bacillus subtilis) (bacterium numbering AS 1.11086) is seeded in flat
After being cultivated twice on plate, cultivated in 250 milliliters of eggplant bottles with nutrient agar, cultivation temperature is 37 DEG C, grown within 36 hours
With under sterilizing washing when good, as the inoculum of seeding tank；
(2) 2/3 dispensing culture medium of seed tank volume is pressed, culture medium composition is：Corn flour 3%, beancake powder 3%, wheat bran
Powder 3%, K2HPO42.15%th, KH2PO40.8%th, MgSO40.075%th, peanut oil 0.3%.Initial pH7.5；
(3) from seeding tank to production tank inoculation, fermentation is started；Fermentation condition：Cultivation temperature is 37 DEG C, and ventilation is 1:
1.1, tank pressure is 0.05mpa/cm2, mixing speed is 220rpm, and spore to be found is generated up to after more than 90%, and liquid fermentation is complete
Into to the adsorbent (explanation that filler and crushing are added in zymocyte liquid：The filler and adsorbent for being added are bacteria fermentation
During general filler and adsorbent), dry to obtain bacillus subtilis solid fungicide.Preparation-obtained withered grass gemma
Bacillus solid fungicide is more than or equal to 1.0 × 10 containing effective bacterium number10Cfu/ grams.
The preparation of the lactobacillus plantarum microbial inoculum of Preparative Example 2
Lactobacillus plantarum strain is inoculated in beef extract-peptone slant medium and cultivates (3 grams of beef extract, peptone 5
~10 grams, 5 grams of NaCl is settled to 1000 milliliters with distillation water dissolves mentioned component, pH value is adjusted to 7), by beef broth peptone
Medium slant seed bacterial strain adds 4 DEG C of interior storages of refrigerators after mineral oil, and activation is taken out twice using preceding, and the bacterial strain after activation is turned
Enter culture in 250 milliliters of eggplant bottles, cultivation temperature is 28 DEG C, is cultivated 48 hours, sterile saline when strain growth is good
Washed down, eluate is the inoculum of seeding tank.Culture medium is added in 1 ton of fermentation tank, consisting of：Corn flour
20kg, beancake powder 20kg, K2HPO4 7.2kg、KH2PO4 5.3kg、MgSO40.5kg, peanut oil 2kg, water 45kg, by above-mentioned group
Divide well mixed, adjust pH value to 6.8.From seeding tank to production tank inoculation, inoculum concentration is 50kg, starts fermentation.Fermentation condition：Training
It is 28 DEG C to support temperature, and tank pressure is 0.05mpa/cm2, periodic agitation, mixing speed is 220rpm, small every 4 after being inoculated with 20 hours
When sampling once, observation thalli growth situation, whether have pollution, after the completion of liquid fermentation, to adding adsorbent in zymocyte liquid
And filler, lactobacillus plantarum microbial inoculum is obtained, effective bacterium number is more than or equal to 1.0 × 1010Cfu/ grams.
The preparation of the bacillus licheniformis agent of Preparative Example 3
The same lactobacillus plantarum microbial inoculum of preparation method of bacillus licheniformis agent, bacillus licheniformis agent (Bacillus
Licheniformis Chinese agriculture Microbiological Culture Collection administrative center, bacterium numbering AS 1.10314, effective viable bacteria) are purchased from
Number is more than or equal to 200,000,000/ml, and miscellaneous bacteria rate is less than or equal to 10%.Preparation-obtained bacillus licheniformis agent contains effective bacterium
Number is more than or equal to 1.0 × 1010Cfu/ grams.
The preparation of the bacillus amyloliquefaciens microbial inoculum of Preparative Example 4
Bacillus amyloliquefaciens microbial inoculum can be prepared according to the method in laboratory, and filler and powder are added after the completion of fermentation
Broken adsorbent, obtains bacillus amyloliquefaciens solid fungicide；Specifically, refer to following methods preparing：
(1) bacillus amyloliquefaciens strain (Bacillus amyloliquefaciens) (is purchased from China Microbiological bacterium
Kind of preservation administration committee common micro-organisms center, bacterium numbering AS1.10901) be seeded in and cultivated twice on flat board after, with battalion
Support agar medium to be cultivated in 100mL/250mL milliliter triangular flask, cultivation temperature is 37 DEG C, with going out during 48 hours well-growns
Under bacterium washing, as the inoculum of seeding tank, shaking speed 200r/min；
(2) 2/3 dispensing culture medium of seed tank volume is pressed, culture medium composition is：Sucrose 0.25%, soyabean expeller powder 1.5%,
MgCl2It is 0.01%, MnSO4It is 0.01%, inoculum concentration 4%, initial pH value of medium 8.0；
(3) coefficient 0.6 in 5.0L fermentation tanks, inoculum concentration 6.67% plants age 18.00h, throughput 1.0L/ (L
Min), mixing speed 500r/min, 35 DEG C of starting fermentation temperature is cooled to 32 DEG C and continues to ferment extremely after fermentation 27h (stationary phase)
End.(explanation：The filler and adsorbent for being added are filler and adsorbent general during bacteria fermentation), must solve
Bacillus amyloliquefacienses microbial inoculum.Preparation-obtained bacillus amyloliquefaciens microbial inoculum containing effective bacterium number more than or equal to 1.0 ×
1010Cfu/ grams.
The preparation of the water-soluble vitamins microbial inoculum of embodiment 1
Each component (unit is weighed by following weight：kg)：
Fulvic acid (leading to Microbial Technics Ltd, trade name " fulvic acid " purchased from Shanghai) 60, amino acid powder (is purchased from
Chemical Co., Ltd. of Xinhua of Sichuan Mianzhu, trade name：" fertilizer grade amino acid powder ") 30, acetic acid 5, molasses 5, Lactobacillus plantarum
Microbial inoculum 1, bacillus laterosporus microbial inoculum 1, bacillus subtilis microbial agent 1, bacillus licheniformis agent 1, bacillus amyloliquefaciens bacterium
Agent 1, water 96.
Preparation method：Each component is well mixed, 30 DEG C of anaerobic fermentations 70 hours are obtained final product.
The preparation of the water-soluble vitamins microbial inoculum of embodiment 2
Each component (unit is weighed by following weight：kg)：
It is fulvic acid 60, amino acid 40, acetic acid 10, molasses 10, lactobacillus plantarum microbial inoculum 1, bacillus laterosporus microbial inoculum 1, withered
Careless gemma bacillus agent 1, bacillus licheniformis agent 1, bacillus amyloliquefaciens microbial inoculum 1, water 74.
Preparation method：Each component is well mixed, 30 DEG C of anaerobic fermentations 70 hours are obtained final product.
The preparation of the water-soluble vitamins microbial inoculum of embodiment 3
Each component (unit is weighed by following weight：kg)：
Fulvic acid 60, amino acid 40, acetic acid 5, molasses 10, lactobacillus plantarum microbial inoculum 2, bacillus laterosporus microbial inoculum 3, withered grass
Gemma bacillus agent 2, bacillus licheniformis agent 2, bacillus amyloliquefaciens microbial inoculum 2, water 67.
Preparation method：Each component is well mixed, 30 DEG C of anaerobic fermentations 70 hours are obtained final product.
The preparation of the water-soluble vitamins microbial inoculum of embodiment 4
Each component (unit is weighed by following weight：kg)：
Bacillus subtilis microbial agent 1, bacillus licheniformis agent 1, bacillus amyloliquefaciens microbial inoculum 1, fulvic acid 60, amino
Acid 30, acetic acid 5, molasses 5, water 65.
Preparation method：Each component is well mixed, 28 DEG C of anaerobic fermentations 60 hours are obtained final product.
The preparation of the water-soluble vitamins microbial inoculum of embodiment 5
Each component (unit is weighed by following weight：kg)：
Bacillus subtilis microbial agent 2, bacillus licheniformis agent 2, bacillus amyloliquefaciens microbial inoculum 2, fulvic acid 70, amino
Acid 40, acetic acid 10, molasses 10, water 100.
Preparation method：Each component is well mixed, 35 DEG C of anaerobic fermentations 80 hours are obtained final product.
The preparation of the water-soluble vitamins microbial inoculum of embodiment 6
Each component (unit is weighed by following weight：kg)：
Bacillus subtilis microbial agent 2, bacillus licheniformis agent 2, bacillus amyloliquefaciens microbial inoculum 2, fulvic acid 60, amino
Acid 40, acetic acid 5, molasses 10, water 67.
Preparation method：Each component is well mixed, 30 DEG C of anaerobic fermentations 70 hours are obtained final product.
The preparation of the water-soluble vitamins microbial inoculum of embodiment 7
Each component (unit is weighed by following weight：kg)：
Bacillus subtilis microbial agent 1, bacillus licheniformis agent 1, bacillus amyloliquefaciens microbial inoculum 1, Lactobacillus plantarum bacterium
Agent 1, bacillus laterosporus microbial inoculum 1, fulvic acid 60, amino acid 30, acetic acid 5, molasses 5, water 65.
Preparation method：Each component is well mixed, 80 hours of 28 DEG C of anaerobic fermentations, is obtained final product.
The preparation of the water-soluble vitamins microbial inoculum of embodiment 8
Each component (unit is weighed by following weight：kg)：
Bacillus subtilis microbial agent 2, bacillus licheniformis agent 2, bacillus amyloliquefaciens microbial inoculum 2, Lactobacillus plantarum bacterium
Agent 2, bacillus laterosporus microbial inoculum 5, fulvic acid 70, amino acid 40, acetic acid 10, molasses 10, water 100.
Preparation method：Each component is well mixed, 60 hours of 35 DEG C of anaerobic fermentations, is obtained final product.
Application effect experiment of the microbial bacterial agent of the present invention of test example 1 in the production of Beijing tomato in plastic greenhouse
First, test material
1st, for examination fertilizer：Microbial bacterial agent prepared by the embodiment of the present invention 1,2,3.
2nd, trial crops：Tomato.
2nd, test site and time
Test site：Daxing institute of agricultural sciences's transformation of scientific and technical result base；
Test period：In November, -2009 in July, 2009.
3rd, test method
Warmhouse booth is experimental field located at, soil is loess, preceding stubble watermelon fruit, soil fertility is common, and plot area is
62.8m2.Experimental field Soil ingenious fertility is shown in Table 1, and trial crops basic condition is shown in Table 2.
The experimental field Soil ingenious fertility of table 1
The trial crops basic condition of table 2
Fertilising basic condition：
Conventional fertilizer application：3500kg/ mus of base manure organic fertilizer, topdresses complex fertilizer 135kg/ mus by Chemical Mixed Fertilizer 50kg/ mus, coordinates
Conventional cultivation control measures.
Determination of yield method：Using meter product mode, yield=density × average individual plant is beared fruit number × mean fruit weight.
Fruit weight assay method：Respectively in fringe fruit, two fringes fruit, Sansui fruit period, taken per fixed point in cell 6 plants it is relative
The fruit of spike number is answered, average fruit weight is calculated.
Agricultural microbial agent cell consumption 0.5kg of the present invention, treatment 1 is present example 1, treatment 2 for the present invention is real
Example 2, treatment 3 are present example 3, and treatment 4 is control conventional fertilizer application+clear water 0.5kg/ mus for the treatment of.
4th, result and analysis
Different disposal tomato yield measurement result table 3.
The different disposal tomato yield measurement result of table 3
As seen from Table 3：Compared with spray clear water control (treatment 4), agricultural microbial agent treatment group of the present invention 1,2,3 kind
Eggplant yield is more obvious than clear water control volume increase, it is possible to increase the utilization rate of soil and fertilizer, reduces fertilizer loss.Wherein the present invention is real
The effect of increasing production for applying the microbial bacterial agent of example 3 (treatment 3) is protruded the most, volume increase 33.7%.
The tamato fruit weight measurement result of different disposal is shown in Table 4.
The different disposal tamato fruit weight measurement result of table 4
As seen from Table 4：Compared with clear water control (treatment 4), agricultural microbial agent treatment group of the present invention 1,2,3 it is average
Single fruit weight is obviously improved；The single fruit weightening of the wherein microbial bacterial agent of the embodiment of the present invention 3 (treatment 3) is protruded the most, and rate of body weight gain is
13.9%.Prove that agricultural microbial agent of the present invention can improve micro-ecological environment around root system, promote root system Nutrient Absorption, produce
Amount and single fruit weight are obviously improved.
Different disposal tamato fruit Vc, soluble solids measurement result are shown in Table 5.
The different disposal tamato fruit Vc of table 5, soluble solids measurement result
As seen from Table 5：Compared with clear water control (treatment 4), the fruit quality of agricultural microbial agent treatment group 3 of the present invention
Significantly improve, especially Vc contents improve 16.4%.
By tomato in plastic greenhouse yield, mean fruit weight, fruit quality index comparing, it was demonstrated that in the feelings that greenhouse fertilizer is sufficient
Under condition, agricultural microbial agent of the present invention, the especially microbial bacterial agent of the embodiment of the present invention 3 can improve soil environment, improve
Crop yield and quality, mainly by improving the microecosystem of soil, strengthen the gas permeability of soil, improve soil water-retaining and protect
Fertile ability, reaches infertile field and supports field, volume increase, the purpose of improve quality.
The Optimal Experimental of the microbial inoculum compatibility of test example 2
Bacillusmusilaginosiengineering, colloid bacillus cereus, side spore bud that routinely the preparation method of microbial inoculum will be bought from market
Spore bacillus, bacillus megaterium, bacillus coagulans, bacillus pumilus, bacillus thuringiensis, lactobacillus acidophilus, German-style
Lactobacillus, the strain of Lactobacillus casei prepare powdering microbial inoculum, living bacteria count >=1.0 × 10 of each microbial inoculum respectively10Cfu/ grams.
Each component (unit is weighed by following weight：kg)：Fulvic acid 60, amino acid 40, acetic acid 5, molasses 10, withered grass gemma
Bacillus microbial inoculum 2, bacillus licheniformis agent 2, bacillus amyloliquefaciens microbial inoculum 2, water 67；
Following microbial inoculum combination (units/kg) is further separately added on the basis of above-mentioned each component：
1st, lactobacillus plantarum microbial inoculum 2, bacillusmusilaginosiengineering microbial inoculum 3；
2nd, lactobacillus plantarum microbial inoculum 2, colloid bacillus cereus microbial inoculum 3；
3rd, lactobacillus plantarum microbial inoculum 2, bacillus laterosporus microbial inoculum 3；
4th, lactobacillus plantarum microbial inoculum 2, bacillus megaterium microbial inoculum 3；
5th, lactobacillus plantarum microbial inoculum 2, bacillus coagulans microbial inoculum 3；
6th, lactobacillus plantarum microbial inoculum 2, bacillus pumilus microbial inoculum 3；
7th, lactobacillus plantarum microbial inoculum 2, bacillus thuringiensis microbial inoculum 3；
8th, lactobacillus acidophilus microbial inoculum 2, colloid bacillus cereus microbial inoculum 3；
9th, lactobacillus acidophilus microbial inoculum 2, bacillus laterosporus microbial inoculum 3；
10th, lactobacillus acidophilus microbial inoculum 2, bacillus megaterium microbial inoculum 3；
11st, lactobacillus acidophilus microbial inoculum 2, bacillus coagulans microbial inoculum 3；
12nd, lactobacillus acidophilus microbial inoculum 2, bacillusmusilaginosiengineering microbial inoculum 3；
13rd, German-style lactobacillus microbial inoculum 2, bacillus laterosporus microbial inoculum 3；
14th, Lactobacillus casei microbial inoculum 2, bacillus laterosporus microbial inoculum 3.
Above-mentioned each component is well mixed, 30 DEG C of anaerobic fermentations 70 hours prepare 14 kinds of microbial bacterial agents respectively, enter
Application effect experiment in the production of row tomato in plastic greenhouse, is experimental field located at warmhouse booth, and soil is loess, preceding stubble watermelon fruit, soil
Earth fertility is common, and plot area is 62.8m2, each microbial bacterial agent cell consumption is 0.5kg, and it is conventional fertilizer application+clear water to compare
0.5kg/ mus for the treatment of, specific experiment method determines the tomato yield and average fruit weight of different disposal with test example 1.
Result of the test is shown in Table 6, table 7.
The different disposal tomato yield measurement result of table 6
The different disposal tamato fruit weight measurement result of table 7
Table 6 and 7 is counted it was demonstrated that the present invention is in bacillus subtilis microbial agent, bacillus licheniformis agent, solution starch gemma bar
On the basis of bacteria agent, the microbial bacterial agent (place prepared by further addition lactobacillus plantarum microbial inoculum and bacillus laterosporus microbial inoculum
3) reason, can significantly improve tomato yield and fruit weight, and compared with control (treatment 15), tomato yield rate of growth reaches
36.1%, improve 19.3-34.3 percentage points than other treatment；Tamato fruit rate of body weight gain is improved up to 15.1% than other treatment
10.2-14.9 percentage points.
Result above proves, bacillus subtilis microbial agent, bacillus licheniformis agent, bacillus amyloliquefaciens microbial inoculum with plant
Microbial bacterial agent prepared by thing lactobacillus microbial inoculum, bacillus laterosporus microbial inoculum compounding can significantly improve tomato yield and fruit weight
Amount, illustrate bacillus subtilis microbial agent, bacillus licheniformis agent, bacillus amyloliquefaciens microbial inoculum and lactobacillus plantarum microbial inoculum,
There is obvious synergistic function between bacillus laterosporus microbial inoculum.
Claims (10)
1. a kind of water-soluble vitamins microbial inoculum, it is characterised in that including following composition：Bacillus subtilis microbial agent, lichens bud
Spore bacillus microbial inoculum, bacillus amyloliquefaciens microbial inoculum, fulvic acid, amino acid, acetic acid and molasses.
2. according to the water-soluble vitamins microbial inoculum described in claim 1, it is characterised in that the weight portion of each composition is：Withered grass bud
1-2 parts of spore bacillus microbial inoculum, bacillus licheniformis agent 1-2 parts, 1-2 parts of bacillus amyloliquefaciens microbial inoculum, fulvic acid 60-70 parts,
Amino acid 30-40 parts, acetic acid 5-10 parts, molasses 5-10 parts.
3. according to the water-soluble vitamins microbial inoculum described in claim 2, it is characterised in that the weight portion of each composition is：Withered grass bud
2 parts of spore bacillus microbial inoculum, 2 parts of bacillus licheniformis agent, 2 parts of bacillus amyloliquefaciens microbial inoculum, 60 parts of fulvic acid, amino acid 40
Part, 5 parts of acetic acid, 10 parts of molasses.
4. according to the water-soluble vitamins microbial inoculum described in claim 1, it is characterised in that also including following component：Plant breast bar
Bacteria agent and bacillus laterosporus microbial inoculum.
5. according to the water-soluble vitamins microbial inoculum described in claim 4, it is characterised in that the weight portion of each composition is：Plant breast
1-2 parts of bacillus microbial inoculum, 1-5 parts of bacillus laterosporus microbial inoculum；
Preferably, the weight portion of each composition is：2 parts of lactobacillus plantarum microbial inoculum, 3 parts of bacillus laterosporus microbial inoculum.
6. the preparation method of water-soluble vitamins microbial inoculum described in a kind of claim 1 to 5 any one, it is characterised in that including
Following steps：Each composition is weighed according to the weight portion, adds water to be well mixed, fermentation is obtained final product.
7. according to the preparation method described in claim 6, it is characterised in that the weight portion of the water is 65-100 parts, preferably
67 parts；
The condition of the fermentation be 28-35 DEG C anaerobic fermentation 60-80 hours；Preferably 30 DEG C anaerobic fermentations 70 hours.
8. water-soluble vitamins microbial inoculum described in claim 1 to 5 any one is improving the soil fertility of facility plastic greenhouse or improvement
Application in ecological environment of soil.
9. water-soluble vitamins microbial inoculum described in claim 1 to 5 any one is improving the crop yield of facility plastic greenhouse or quality
In application.
10. according to the application described in claim 9, it is characterised in that the crop is preferably tomato.
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