CN110330370A - While a kind of turf biological organic fertilizer and its preparation method and application containing trichoderma and bacillus amyloliquefaciens - Google Patents
While a kind of turf biological organic fertilizer and its preparation method and application containing trichoderma and bacillus amyloliquefaciens Download PDFInfo
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- CN110330370A CN110330370A CN201910661199.5A CN201910661199A CN110330370A CN 110330370 A CN110330370 A CN 110330370A CN 201910661199 A CN201910661199 A CN 201910661199A CN 110330370 A CN110330370 A CN 110330370A
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- turf
- trichoderma
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a kind of turf biological organic fertilizers and its preparation method and application containing trichoderma and bacillus amyloliquefaciens simultaneously.The present invention is using turf as raw material, bacillus amyloliquefaciens SQR9 or Guizhou trichoderma NJAU4742 progress compost fermentation is added and prepares biological organic fertilizer, the carbon in turf has sufficiently been activated using bacterial strain SQR9 or NJAU4742, active humic acid content is promoted by 3.6% to 13.5% or more, it is made to be changed into " carbon living " that biology can utilize by biological unserviceable " dead carbon "；The turf biological organic fertilizer of preparation can dramatically increase crop plant height, stem thickness, promote plant growth, can significantly improve and improve crop and fruit quality.
Description
Technical field
The invention belongs to technical field of biological organic fertilizer, are related to a kind of turf containing trichoderma and bacillus amyloliquefaciens simultaneously
Biological organic fertilizer and its preparation method and application.
Background technique
Turf is also known as peat, and mud coal is the product of formation process of bog, containing the plant residue not being decomposed largely,
Humus, cellulose, lignin and a part of minerals, rich in nutrients such as N P and Ks.In addition, also containing some lifes in turf
Active material is managed, is a kind of sterile, nontoxic, nuisanceless, pollution-free, noresidue green matter.
China's turf is resourceful, and in addition to a small number of arid areas not yet find turf resource, national each province and city area is almost
There is turf resource.In agricultural production, turf can be used following with mode: 1, being directly used as fertilizer to improve soil；2,
Organic and inorganic fertilizer mixed fertilizer is blended into chemical fertilizer；3, sodium humate is developed with alkali process or extract the humic acid inside turf
To develop humic acid fertilizer；4, turf is used as medium to adsorb microorganism and produce bacterial manure etc.；5, with rock wool, coconut husk fermentation material,
The compounds such as vermiculite develop seedling medium etc..In existing Land use systems, there are also to be strengthened in terms of giving full play to its component function.
If the main component in turf is carbon, the carbon of this high molecular weight is either manured into soil, or as the adsorbent of microorganism,
All it is difficult to be utilized by the microorganism in a short time.If certain technique can be used, the carbon in turf is activated using microorganism,
Good application prospect will be had.
Summary of the invention
The purpose of the present invention is be directed to the above-mentioned deficiency of the prior art, provide a kind of while conciliating starch gemma bar containing trichoderma
The preparation method of the turf biological organic fertilizer of bacterium.
It is a further object of the present invention to provide the turf biologies containing trichoderma and bacillus amyloliquefaciens of this method preparation to have
Machine fertilizer.
It is yet another object of the invention to provide the applications of the turf biological organic fertilizer containing trichoderma and bacillus amyloliquefaciens.
The purpose of the present invention can be achieved through the following technical solutions:
The preparation method of turf biological organic fertilizer containing trichoderma and bacillus amyloliquefaciens, the preparation method be selected from
Any one lower method:
Method one the following steps are included:
(1) suitable quantity of water and alkaline matter adjustment turf pH to 4.5~6.5 are added into turf, carbon source is added, amino is added
Sour dilution adjusts water content to 40%~50%, accesses the Guizhou trichoderma that deposit number is CGMCC NO.12166
Aerobic compost is carried out after (Trichoderma guizhouense) NJAU4742 fermentation liquid, is fermented 7~20 days and is obtained containing trichoderma
Turf biological organic fertilizer；
(2) suitable quantity of water is added into turf and alkaline matter adjusts turf pH to 6.5~7.5, carbon source is added, amino is added
Sour dilution adjusts water content to 40%~50%, accesses the bacillus amyloliquefaciens that deposit number is CGMCC NO.5808
Aerobic compost is carried out after (Bacillus amyloliquefaciens) SQR9 seed liquor, is fermented 7~20 days and is obtained the starch containing solution
The turf biological organic fertilizer of bacillus；
(3) the turf biological organic fertilizer by described containing trichoderma and the turf biological organic fertilizer containing bacillus are pressed
It is uniformly mixed according to the ratio of 1:0.5~1.5, obtains the turf biological organic fertilizer containing trichoderma and bacillus amyloliquefaciens；
Method two the following steps are included:
(1) suitable quantity of water and alkaline matter adjustment turf pH to 4.5~6.5 are added into turf, carbon source is added, amino is added
Sour dilution adjusts water content to 40%~50%, accesses the Guizhou trichoderma that deposit number is CGMCC NO.12166
Aerobic compost is carried out after (Trichoderma guizhouense) NJAU4742 fermentation liquid, is fermented 7~20 days and is obtained containing trichoderma
Turf biological organic fertilizer；
(2) spore quantity is reached 1010A mL-1Bacillus amyloliquefaciens SQR9 liquid spawn add by 0.2% amount
Enter in the turf biological organic fertilizer to described containing trichoderma, mix well, control moisture is lower than 30%, obtains the reconciliation containing trichoderma and forms sediment
The turf biological organic fertilizer of afnyloliquefaciens；
Method three includes the following steps
(1) suitable quantity of water is added into turf and alkaline matter adjusts turf pH to 6.5~7.5, carbon source is added, amino is added
Sour dilution adjusts water content to 40%~50%, accesses the bacillus amyloliquefaciens that deposit number is CGMCC NO.5808
Aerobic compost is carried out after (Bacillus amyloliquefaciens) SQR9 seed liquor, is fermented 7~20 days and is obtained the starch containing solution
The turf biological organic fertilizer of bacillus；
(2) Guizhou trichoderma NJAU4742 direct fermentation crop material is utilized according to method disclosed in 201610151640.1
Trichoderma solid spawn is prepared, trichoderma concentration is 10 in prepared trichoderma solid spawn8cfu/ml；
(3) the trichoderma solid spawn of step (2) preparation is added to the life of the turf containing bacillus amyloliquefaciens by 5% amount
It in organic fertilizer, mixes well, control moisture is lower than 30%, obtains the turf biological organic containing trichoderma and bacillus amyloliquefaciens
Fertilizer.
Wherein, the turf biological organic fertilizer preparation method containing trichoderma preferably includes: suitable quantity of water being added into turf
With alkaline matter adjustment turf pH to 4.5~6.5, carbon source is added thereto after pH stablizes, amino acid dilution is added and adjusts
Water content mixes to 40%~50%, stands 20~24 hours, the Guizhou trichoderma that deposit number is CGMCCNO.12166 is added
(Trichoderma guizhouense) NJAU4742 fermentation liquid is stacked after mixing well and carries out aerobic compost, fermentation at pile
7~20 days turf biological organic fertilizers of the acquisition containing trichoderma；Wherein the additional amount of the carbon source is the turf quality adjusted after pH
5%~10%；Effective bacterium number amount reaches 10 in the NJAU4742 fermentation liquid9CFU·mL-1, NJAU4742 fermentation liquid
Additive amount is adjust the turf weight after pH 5%~15%.
The turf biological organic fertilizer preparation method containing bacillus amyloliquefaciens preferably includes: being added into turf suitable
It measures water and alkaline matter adjusts turf pH to 6.5~7.5, carbon source is added thereto after pH stablizes, amino acid dilution is added
Water content is adjusted to 40%~50%, 20~24 hours is stood, the solution starch gemma bar that deposit number is CGMCC NO.5808 is added
Bacterium (Bacillus amyloliquefaciens) SQR9 seed liquor stacks after mixing well and carries out aerobic compost, hair at pile
Ferment 7~20 days turf biological organic fertilizers of the acquisition containing bacillus amyloliquefaciens；Wherein the additional amount of the carbon source is to adjust pH
The 5%~10% of turf quality afterwards；Effective bacterium number amount reaches 10 in SQR9 seed liquor9CFU·mL-1, SQR9 seed liquor adds
Dosage is adjust the turf weight after pH 5%~15%.
The bacillus amyloliquefaciens SQR9 liquid spawn is preferably prepared by the following method to obtain: will be stored in -80
Bacillus amyloliquefaciens SQR9 strain in DEG C refrigerator glycerol tube, activation of crossing on LB solid medium, is placed in 37 DEG C of cultures
Overnight incubation in case, inoculation SQR9 single bacterium drop down onto LB liquid medium, are subsequently placed in 30 DEG C, shake 12h on 170rpm shaking table, then
It is inoculated into after LB liquid medium by 0.1% (v/v) and shakes 36h and obtain SQR9 fermentation liquid, effective bacterium number amount reaches 109CFU·
mL-1, spare.
The alkaline matter is plant ash.When preparing the turf biological organic fertilizer containing trichoderma, the additive amount of plant ash is excellent
The 5%~10% of turf quality is selected, the pH of turf is adjusted to 4.5~6.5；In turf of the preparation containing bacillus amyloliquefaciens
The 12%~20% of the preferred turf quality of the additive amount of plant ash when biological organic fertilizer, the pH of turf is adjusted to 6.5~
7.5, convenient for more acidolysis amino acid solution can be added below, be conducive to microorganism growth, be conducive to the carbon activation in turf.
Water to turf heap body water content is added in the preparation method when preferably adjusting pH be 30%~40%, and water content is high
Point is conducive to shorten the acid-base neutralization time, as early as possible stable pH value.
The preferred rice bran of the carbon source or powdered rice hulls.A small amount of available carbon source is initially provided to added function bacterium,
Allow microbial reproduction, microorganism, gradually using the carbon in turf, the carbon in turf is activated to come during breeding.
The full price aminoacids solution that the amino acid dilution is preferably diluted with water has in turf biology of the preparation containing trichoderma
When machine fertilizer, the additional amount of full price aminoacids solution is adjust the turf weight after pH 5%~10%；In preparation full price aminoacids solution
Additional amount be adjust pH after turf weight 3%~8%.The full price aminoacids solution can be prepared in accordance with the following methods
Form: (1) in closed container first dead livestock and poultry animal through automation is crushed, all solid contents and liquid be transferred to automatically it is closed
Hydrolytic decomposition pot after, in initial acid c (1/2H2SO4) it is 2.5-5mol L-1, hydrolyze 2- under 80-100 DEG C and 1-2 atmospheric pressure
5 hours；(2) hydrolysis terminates when solution in hydrolytic decomposition pot is cooled to 80 DEG C or less, and stratification collects the amino acid solution in middle layer
To obtain the final product.Amino acid hydrolyticsolution contains amino acid and various peptide matters about 10% (g/100ml) or more；Containing 17 kinds of amino acid, small
Peptide and a small amount of polypeptide, solution are in highly acid, free aminoacid content >=100gL-1.Jiangsu Province Jiangyin can also directly be bought
The animal protein amino acid Water soluble fertilizer of Lian Ye Biotechnology Co., Ltd, city production.
The preparation method is preferred, work as when aerobic compost heap temperature rise to 50 DEG C of progress first time turnings；Composting mistake
Cheng Zhong carries out turning when heap temperature no longer rises.
A length of 7~15 days when the compost of the method preferred spring or summer；A length of 15 when the compost in autumn or winter
~20 days.
The turf biological organic fertilizer containing trichoderma and bacillus amyloliquefaciens prepared according to the method described in the present invention.
Turf biological organic fertilizer of the present invention is promoting plant growth and/or is improving the application in crop quality.
The utility model has the advantages that
The present invention is added bacillus amyloliquefaciens SQR9 or Guizhou trichoderma NJAU4742 and carries out compost hair using turf as raw material
Ferment prepares biological organic fertilizer, has sufficiently activated the carbon in turf using bacterial strain SQR9 or NJAU4742, active humic acid content by
3.6% is promoted to 13.5% or more, it is made to be changed into " carbon living " that biology can utilize by biological unserviceable " dead carbon "；System
Standby turf biological organic fertilizer can dramatically increase crop plant height, stem thickness, promote plant growth, can significantly improve and improve crop
Fruit quality.
1 turf Contents of Main Components (original) of table
2 turf Contents of Main Components of table (after SQR9 activation)
3 turf Contents of Main Components of table (after NJAU4742 activation)
Specific embodiment
Material used in the following embodiment:
Turf is provided by Shaanxi Hua Weifei industry development in science and technology Co., Ltd, basic physicochemical character: organic matter 70.6%, pH
3.8, full nitrogen 1.3%, full phosphorus 2.2%, full potassium 0.3%, moisture 16.4%.Total humic acid 20.2% (water-insoluble 0.2%, richness
In acid 0.7%, humic acid 2.7%, humin 16.6%), active humic acid 3.6%.
Gained after plant ash is burned by wheat stalk, pH value 8.90.
Full price amino acid (AA): it is provided by Jiangyin Lian Ye Biotechnology Co., Ltd.Full price amino acid is by giving up
It abandons livestock and poultry to form through acidolysis, contains 17 kinds of amino acid, small peptide and a small amount of polypeptide, solution is in highly acid, and pH value is after 250 times of dilution
2.5, free aminoacid content >=100gL-1。
The bacterial strain of the bacillus amyloliquefaciens SQR9 of CGMCC NO.5808 discloses in CN104130040A；CGMCC
The bacterial strain of the Guizhou trichoderma NJAU4742 of NO.12166 discloses in ZL2018101301042.
Embodiment 1
The preparation of bacterial strain NJAU4742 turf biological organic fertilizer:
It will pour into sterile petri dish after the solid PDA medium heating and melting prepared, be inoculated with after it is solidified again
Trichoderma NJAU4742 is continuously cultivated 6~7 days in 28 DEG C of constant incubators, after it covers with entire plate, is placed in 4 DEG C of refrigerators
It saves, for use.
Trichoderma fungus block of the same size is taken on the plate for the PDA for covering with trichoderma with punch, is seeded in PDA Liquid Culture
In base, at 28 DEG C, the product under the conditions of 175rpm after continuous oscillation culture 6 days is trichoderma fermentation liquid, and blood cell is used after diluting
It is 2 × 10 that tally, which measures reesei spores number,7CFU·mL-1.For use.
Suitable quantity of water (15kg) is added into 50kg turf, with plant ash (dosage is the 10% of turf dosage) adjustment turf pH
To 6.17,5kg rice bran is added thereto after pH stablizes, full price amino acid is added, and (dosage is the turf dosage adjusted after pH value
7%) dilution adjust water content to 50%, mix well, static 24 hours.The turf weight being equivalent to after adjusting pH is added
Trichoderma harzianum (Trichoderma harzianum) NJAU4742 Fermented Condensed liquid of 10% CGMCC NO.12166 of amount,
It is stacked after mixing well and carries out aerobic compost at pile, when heap temperature rise to 50 DEG C of progress first time turnings；During composting, heap temperature
Turning is carried out when no longer rising, ferment 17 days the turf biological organic fertilizer obtained containing trichoderma.
The preparation of SQR9 turf biological organic fertilizer:
The bacillus amyloliquefaciens SQR9 strain that will be stored in -80 DEG C of refrigerator glycerol tubes is drawn on LB solid medium
Line activation, is placed in overnight incubation in 37 DEG C of incubators, and the SQR9 single colonie on plate is gently scraped with oese and then is put into
In LB liquid medium, it is subsequently placed in 30 DEG C, shakes 12h on 170rpm shaking table, then is inoculated into liquid LB culture by 0.1% (v/v)
36h is shaken after base to obtain SQR9 fermentation liquid (effective bacterium number amount reaches 109CFU·mL-1), it is spare.
Suitable quantity of water (15kg) is added into 50kg turf, with plant ash (dosage is the 14% of turf dosage) adjustment turf pH
To 6.92,5kg rice bran is added thereto after pH stablizes, full price amino acid is added, and (dosage is the turf dosage adjusted after pH value
5%) dilution adjust water content to 50%, mix well, static 24 hours.The turf weight being equivalent to after adjusting pH is added
10% deposit number of amount is the bacillus amyloliquefaciens (Bacillus amyloliquefaciens) of CGMCC NO.5808
SQR9 Fermented Condensed bacterium solution stacks after mixing well and carries out aerobic compost at pile, when heap temperature rise to 50 DEG C of progress is turned over for the first time
Heap；During composting, turning is carried out when heap temperature no longer rises, ferment the turf biology obtained containing bacillus amyloliquefaciens for 15 days
Organic fertilizer.
Turf biological organic fertilizer containing trichoderma and the turf biological organic fertilizer containing bacillus amyloliquefaciens are pressed to 50% amount
Mix well, control moisture be lower than 30%, as simultaneously the turf biological organic fertilizer containing trichoderma and bacillus amyloliquefaciens.
The turf biological organic fertilizer containing trichoderma and bacillus amyloliquefaciens meets NY 884-2012 standard simultaneously for this, leads
Want index as follows: function bacterium (Trichoderma harzianum NJAU4742+ bacillus amyloliquefaciens SQR9) quantity is 8.3 × 107CFU·g-1Fertilizer
Expect dry weight, organic matter 55.0%, humic acid total content 18.9%, active humic acid content 14.2%, moisture 27.5%, pH value
6.0, excrement colibacillus group number is not detected, no roundworm egg, validity period >=6 month.Arsenic 0.019mg/kg, cadmium 1.95mg/kg, lead
14.2mg/kg, chromium 15.6mg/kg, mercury 0.053mg/kg.
Embodiment 2
The preparation of bacterial strain NJAU4742 turf biological organic fertilizer:
It will pour into sterile petri dish after the solid PDA medium heating and melting prepared, be inoculated with after it is solidified again
Trichoderma NJAU4742 is continuously cultivated 6~7 days in 28 DEG C of constant incubators, after it covers with entire plate, is placed in 4 DEG C of refrigerators
It saves, for use.
Trichoderma fungus block of the same size is taken on the plate for the PDA for covering with trichoderma with punch, is seeded in PDA Liquid Culture
In base, at 28 DEG C, the product under the conditions of 175rpm after continuous oscillation culture 6 days is trichoderma fermentation liquid, and blood cell is used after diluting
It is 2 × 10 that tally, which measures reesei spores number,7CFU·mL-1.For use.
Suitable quantity of water (15kg) is added into 50kg turf, with plant ash (dosage is the 10% of turf dosage) adjustment turf pH
To 6.17,5kg rice bran is added thereto after pH stablizes, full price amino acid is added, and (dosage is the turf dosage adjusted after pH value
7%) dilution adjust water content to 50%, mix well, static 24 hours.The turf weight being equivalent to after adjusting pH is added
Trichoderma harzianum (Trichoderma harzianum) NJAU4742 Fermented Condensed liquid of 10% CGMCC NO.12166 of amount,
It is stacked after mixing well and carries out aerobic compost at pile, when heap temperature rise to 50 DEG C of progress first time turnings；During composting, heap temperature
Turning is carried out when no longer rising, ferment 17 days the turf biological organic fertilizer obtained containing trichoderma, to produce the turf biology containing trichoderma
Organic fertilizer.
The production of SQR9 liquid fermentation strain:
The bacillus amyloliquefaciens SQR9 strain that will be stored in -80 DEG C of refrigerator glycerol tubes is drawn on LB solid medium
Line activation, is placed in overnight incubation in 37 DEG C of incubators, and the SQR9 single colonie on plate is gently scraped with oese and then is put into
In LB liquid medium, it is subsequently placed in 30 DEG C, shakes 12h on 170rpm shaking table, then is inoculated into liquid LB culture by 0.1% (v/v)
36h is shaken after base to obtain SQR9 fermentation liquid (effective bacterium number amount reaches 109CFU·mL-1), it is spare.
Continue that bacillus amyloliquefaciens SQR9 strain is inoculated into LB liquid medium by 10% inoculum concentration, is fermenting
Ferment (30 DEG C of temperature, revolving speed 170rpm) 36h in tank, and obtaining bacterial strain SQR9 liquid spawn, (spore quantity reaches 1.6 × 1010
A mL-1), it is spare.
SQR9 fermentation liquid is added to the turf biological organic fertilizer containing trichoderma by 0.2% amount, is mixed well, moisture is controlled
Lower than 30%, as simultaneously the turf biological organic fertilizer containing trichoderma and bacillus amyloliquefaciens.
The turf biological organic fertilizer meets NY 884-2012 standard, and main indicator is as follows: function bacterium (Trichoderma harzianum
NJAU4742+ bacillus amyloliquefaciens SQR9) quantity be 9.6 × 107CFU·g-1Fertilizer dry weight, organic matter 53.3%, humic acid
Total content 18.9%, active humic acid content 14.2%, moisture 27.1%, pH value 5.9, excrement colibacillus group number are not detected, no ascarid
Worm's ovum, validity period >=6 month.Arsenic 0.019mg/kg, cadmium 1.95mg/kg, lead 14.2mg/kg, chromium 15.6mg/kg, mercury
0.053mg/kg。
Embodiment 3
The preparation of SQR9 turf biological organic fertilizer:
The bacillus amyloliquefaciens SQR9 strain that will be stored in -80 DEG C of refrigerator glycerol tubes is drawn on LB solid medium
Line activation, is placed in overnight incubation in 37 DEG C of incubators, and the SQR9 single colonie on plate is gently scraped with oese and then is put into
In LB liquid medium, it is subsequently placed in 30 DEG C, shakes 12h on 170rpm shaking table, then is inoculated into liquid LB culture by 0.1% (v/v)
36h is shaken after base to obtain SQR9 fermentation liquid (effective bacterium number amount reaches 109CFU·mL-1), it is spare.
Suitable quantity of water (15kg) is added into 50kg turf, with plant ash (dosage is the 14% of turf dosage) adjustment turf pH
To 6.92,5kg rice bran is added thereto after pH stablizes, full price amino acid is added, and (dosage is the turf dosage adjusted after pH value
5%) dilution adjust water content to 50%, mix well, static 24 hours.The turf weight being equivalent to after adjusting pH is added
10% deposit number of amount is the bacillus amyloliquefaciens (Bacillus amyloliquefaciens) of CGMCC NO.5808
SQR9 Fermented Condensed bacterium solution stacks after mixing well and carries out aerobic compost at pile, when heap temperature rise to 50 DEG C of progress is turned over for the first time
Heap；During composting, turning is carried out when heap temperature no longer rises, ferment the turf biology obtained containing bacillus amyloliquefaciens for 15 days
Organic fertilizer.
Bacterial strain NJAU4742 solid fermentation produces strain:
The plate culture of trichoderma NJAU 4742: sterile culture will be poured into after the solid PDA medium heating and melting prepared
In ware, it is inoculated with trichoderma NJAU4742 after it is solidified again, continuously cultivates 6~7 days, is covered with to it whole in 28 DEG C of constant incubators
After a plate, it is placed in 4 DEG C of refrigerators and saves, for use.
The preparation of 4742 fermentation liquid of trichoderma NJAU: it is taken on the plate for the PDA for covering with trichoderma with punch of the same size
Trichoderma fungus block, is seeded in PDA liquid medium, and at 28 DEG C, the product under the conditions of 175rpm after continuous oscillation culture 6 days is
Trichoderma fermentation liquid, measuring reesei spores number with blood counting chamber after diluting is 2 × 107CFU·mL-1。
The solid spawn of bacterial strain NJAU4742 produces: seeing a kind of (the trichoderma direct fermentation crop straw of ZL 201610151640.1
Stalk prepare trichoderma solid spawn method and preparation product) embodiment 1 bacterial strain NJAU4742 solid spawn.
The solid spawn of NJAU4742 is added to the turf biological organic fertilizer containing bacillus amyloliquefaciens by 5% amount
In, mix well, control moisture be lower than 30%, as simultaneously the turf biological organic fertilizer containing trichoderma and bacillus amyloliquefaciens.
The turf biological organic fertilizer meets NY 884-2012 standard, and main indicator is as follows: function bacterium (Trichoderma harzianum
NJAU4742+ bacillus amyloliquefaciens SQR9) quantity be 8.5 × 107CFU·g-1Fertilizer dry weight, organic matter 54.0%, humic acid
Total content 17.2%, active humic acid content 12.8%, moisture 25.9%, pH value 6.1, excrement colibacillus group number are not detected, no ascarid
Worm's ovum, validity period >=6 month.Arsenic 0.019mg/kg, cadmium 1.95mg/kg, lead 14.2mg/kg, chromium 15.6mg/kg, mercury
0.053mg/kg。
Turf biological organic fertilizer biological effect of the embodiment 4 containing trichoderma and bacillus amyloliquefaciens
4.1 for studying object
Tomato；Kind: century red hat.
4.2 for trying fertilizer
Turf biological organic fertilizer (prepared by embodiment 1, embodiment 2, embodiment 3) containing trichoderma and bacillus amyloliquefaciens,
Chemical fertilizer (urea, dipotassium hydrogen phosphate, calcium superphosphate).
4.3 for trying soil
Yellow soil, basic physical and chemical are organic matter: 41.5g/kg；Full nitrogen: 3.31g/kg；Full phosphorus: 3.31g/kg；Speed
Imitate phosphorus: 37.9mg/kg；Available potassium: 521.0mg/kg；pH:6.58.
4.4 test design
Four processing are arranged in field experiment: 1 (CK1) of processing: not applying fertilizer；Handle 2 (CK2): Single fertilizer (conventional amount used)；
3 (AOF) of processing: (conventional amount used subtracts contained by the turf biological organic fertilizer after inactivation turf biological organic fertilizer+chemical fertilizer after inactivation
Nutrient)；Handle 4 (BOF): turf biological organic fertilizer+chemical fertilizer (conventional amount used subtracts nutrient contained by turf biological organic fertilizer).Each
It handles in triplicate, each processing plot area 22.5m2(long 15.0m, 1.5 meters wide).
4.5 test method
Nursery: choosing full grains, century red hat seed of uniform size, and every bunch planting 3 stays 1 plant of healthy seedling after emergence,
Conventional water and fertilizer management.
Crop field fertilising is with management: each processing nitrogen, phosphorus and potassium fertilizer base manure amount of application is identical, i.e., and urea 21kg/ mus, calcium superphosphate
68kg/ mus, potassium sulfate: 16.5kg/ mus, turf biological organic fertilizer amount of application: every cell applies 3.5kg (dry weight) fertilizer, does
Base manure application, conventional liquid manure and plant protection management.
When tomato seedling length to 1 three leaf of bud, the seedling for selecting growing way almost the same was transplanted to greatly on March 20th, 2018
Field；On April 16th, 2018 and May 6 spray emamectin benzoate class pesticide control blueness worm, do not spray other pesticides, on May 16th, 2018
Start meter to produce.
Data processing carries out in 2016 software of office with production chart, and data analysis and significance test of difference exist
It is carried out in SPSS25.0 software.
4.6 test result
4.6.1 influence of the application turf biological organic fertilizer to tomato biological character
Influence of the different disposal to the SPAD of tomato plant height, stem thickness and blade is different (table 1, table 1, table 3).It is raw to apply turf
Organic fertilizer (BOF) can dramatically increase the plant height of tomato plant, and BOF is significantly better than Single fertilizer (CK2) and (the CK1) (P that do not apply fertilizer
=0.05), it is better than AOF；Application turf biological organic fertilizer (BOF) can obviously increase the stem thickness of tomato plant, be significantly better than CK2,
CK1 and AOF (P=0.05)；Application composite microbiological fertilizer (BOF) can significantly improve the SPAD value of tomato leaf.Show to apply
Turf biological organic fertilizer can significantly improve and improve the biological character of tomato.
The influence of 1 turf biological organic fertilizer of table hat biological character and structure of output red to century
Note: it CK1: does not apply fertilizer；CK2: Single fertilizer (conventional amount used)；
AOF1: (conventional amount used subtracts the grass after inactivation to turf biological organic fertilizer (preparation of embodiment 1)+chemical fertilizer after inactivation
Nutrient contained by charcoal biological organic fertilizer)；
BOF1: (conventional amount used subtracts contained by turf biological organic fertilizer+chemical fertilizer turf biological organic fertilizer (preparation of embodiment 1)
Nutrient)；
The influence of 2 turf biological organic fertilizer of table hat biological character and structure of output red to century
Note: it CK1: does not apply fertilizer；CK2: Single fertilizer (conventional amount used)；
AOF2: (conventional amount used subtracts the grass after inactivation to turf biological organic fertilizer (preparation of embodiment 2)+chemical fertilizer after inactivation
Nutrient contained by charcoal biological organic fertilizer)；
BOF2: (conventional amount used subtracts contained by turf biological organic fertilizer+chemical fertilizer turf biological organic fertilizer (preparation of embodiment 2)
Nutrient)；
The influence of 3 turf biological organic fertilizer of table hat biological character and structure of output red to century
Note: it CK1: does not apply fertilizer；CK2: Single fertilizer (conventional amount used)；
AOF3: (conventional amount used subtracts the grass after inactivation to turf biological organic fertilizer (preparation of embodiment 3)+chemical fertilizer after inactivation
Nutrient contained by charcoal biological organic fertilizer)；
BOF3: (conventional amount used subtracts contained by turf biological organic fertilizer+chemical fertilizer turf biological organic fertilizer (preparation of embodiment 3)
Nutrient)；
4.6.2 influence of the application turf biological organic fertilizer to Quality of Tomato Fruit
Influence of the different disposal to the Vc content of tamato fruit, organic acid content and soluble sugar content different (tables 4, table
5, table 6).Application turf biological organic fertilizer (BOF) can dramatically increase the soluble sugar content of tamato fruit, and wherein BOF is significantly excellent
In CK2, CK1 and AOF (P=0.05), increase soluble sugar content and organic acid content in tamato fruit ratio (BOF > AOF >
CK1 > CK2), show that turf biological organic fertilizer can improve Quality of Tomato Fruit.
The influence of 4 turf biological organic fertilizer of table hat fruit quality red to century
Note: it CK1: does not apply fertilizer；CK2: Single fertilizer (conventional amount used)；
AOF1: (conventional amount used subtracts the grass after inactivation to turf biological organic fertilizer (preparation of embodiment 1)+chemical fertilizer after inactivation
Nutrient contained by charcoal biological organic fertilizer)；
BOF1: (conventional amount used subtracts contained by turf biological organic fertilizer+chemical fertilizer turf biological organic fertilizer (preparation of embodiment 1)
Nutrient)；
The influence of 5 turf biological organic fertilizer of table hat fruit quality red to century
Note: it CK1: does not apply fertilizer；CK2: Single fertilizer (conventional amount used)；
AOF2: (conventional amount used subtracts the grass after inactivation to turf biological organic fertilizer (preparation of embodiment 2)+chemical fertilizer after inactivation
Nutrient contained by charcoal biological organic fertilizer)；
BOF2: (conventional amount used subtracts contained by turf biological organic fertilizer+chemical fertilizer turf biological organic fertilizer (preparation of embodiment 2)
Nutrient)；
The influence of 6 turf biological organic fertilizer of table hat fruit quality red to century
Note: it CK1: does not apply fertilizer；CK2: Single fertilizer (conventional amount used)；
AOF3: (conventional amount used subtracts the grass after inactivation to turf biological organic fertilizer (preparation of embodiment 3)+chemical fertilizer after inactivation
Nutrient contained by charcoal biological organic fertilizer)；
BOF3: (conventional amount used subtracts contained by turf biological organic fertilizer+chemical fertilizer turf biological organic fertilizer (preparation of embodiment 3)
Nutrient)；
4.6.3 influence of the application turf biological organic fertilizer to tamato fruit yield
Different disposal is different to tamato fruit yield effect, and application turf biological organic fertilizer can significantly improve cucumber fruits
Yield (table 7, table 8, table 9).By taking table 7 as an example (table 8, table 9 have similar rule): application turf biological organic fertilizer does not apply fertilizer than
Increase production 1186.7 kilograms per acre, rate of growth 32.9%；Increase production 533.3 kilograms per acre than Single fertilizer, rate of growth 12.5%, than
AOF increases production 506.7 kilograms per acre, rate of growth 11.8%.
Variance analysis discovery further is carried out to the yield result of table 7, F (1376.18) > F 0.01 (9.78) between handling
>F0.05(4.76)；And F (5.12) < F0.05 (5.14) < F0.01 (10.92) between repeating, that is, the significant (P of otherness between handling
=0.05) difference is not significant at P=0.05 and P=0.01 in, repeating.Meanwhile comparing the difference between processing between repetition
Property discovery, F handle (1376.18) > F repeat (5.12), that is, handle between significant difference be greater than repeat between variation, show difference
There are significant differences for influence of the fertilizer treatment for tamato fruit yield.
Yield is carried out to handle an otherness significance analysis, the results showed that at P=1%, BOF and other processing ratios
Relatively all have significant difference.
Table 7 applies influence of the NJAU4742 turf biological organic fertilizer to tamato fruit yield
Note: it CK1: does not apply fertilizer；CK2: Single fertilizer (conventional amount used)；
AOF1: (conventional amount used subtracts the life after inactivation to turf biological organic fertilizer (preparation of embodiment 1)+chemical fertilizer after inactivation
Nutrient contained by organic fertilizer)；
BOF1: (conventional amount used is subtracted and is supported contained by biological organic fertilizer turf biological organic fertilizer (preparation of embodiment 1)+chemical fertilizer
Point)；
Table 8 applies influence of the NJAU4742 turf biological organic fertilizer to tamato fruit yield
Note: it CK1: does not apply fertilizer；CK2: Single fertilizer (conventional amount used)；
AOF1: (conventional amount used subtracts the life after inactivation to turf biological organic fertilizer (preparation of embodiment 2)+chemical fertilizer after inactivation
Nutrient contained by organic fertilizer)；
BOF1: (conventional amount used is subtracted and is supported contained by biological organic fertilizer turf biological organic fertilizer (preparation of embodiment 2)+chemical fertilizer
Point)；
Table 9 applies influence of the NJAU4742 turf biological organic fertilizer to tamato fruit yield
Note: it CK1: does not apply fertilizer；CK2: Single fertilizer (conventional amount used)；
AOF3: (conventional amount used subtracts the life after inactivation to turf biological organic fertilizer (preparation of embodiment 3)+chemical fertilizer after inactivation
Nutrient contained by organic fertilizer)；
BOF3: (conventional amount used is subtracted and is supported contained by biological organic fertilizer turf biological organic fertilizer (preparation of embodiment 3)+chemical fertilizer
Point).
Claims (12)
1. the preparation method of the turf biological organic fertilizer containing trichoderma and bacillus amyloliquefaciens, which is characterized in that the preparation
Method is selected from any one following method:
Method one the following steps are included:
(1) suitable quantity of water and alkaline matter adjustment turf pH to 4.5~6.5 are added into turf, carbon source is added, it is dilute that amino acid is added
It releases liquid and adjusts water content to 40%~50%, access the Guizhou trichoderma (Trichoderma that deposit number is CGMCC NO.12166
Guizhouense aerobic compost) is carried out after NJAU4742 fermentation liquid, 7~20 days turf biological organics of the acquisition containing trichoderma of fermenting
Fertilizer；
(2) suitable quantity of water is added into turf and alkaline matter adjusts turf pH to 6.5~7.5, carbon source is added, it is dilute that amino acid is added
It releases liquid and adjusts water content to 40%~50%, access the bacillus amyloliquefaciens (Bacillus that deposit number is CGMCC NO.5808
Amyloliquefaciens aerobic compost) is carried out after SQR9 seed liquor, is fermented 7~20 days and is obtained containing bacillus amyloliquefaciens
Turf biological organic fertilizer；
(3) the turf biological organic fertilizer by described containing trichoderma and the turf biological organic fertilizer containing bacillus are according to 1:
0.5~1.5 ratio is uniformly mixed, and obtains the turf biological organic fertilizer containing trichoderma and bacillus amyloliquefaciens；
Method two the following steps are included:
(1) suitable quantity of water and alkaline matter adjustment turf pH to 4.5~6.5 are added into turf, carbon source is added, it is dilute that amino acid is added
It releases liquid and adjusts water content to 40%~50%, access the Guizhou trichoderma (Trichoderma that deposit number is CGMCC NO.12166
Guizhouense aerobic compost) is carried out after NJAU4742 fermentation liquid, 7~20 days turf biological organics of the acquisition containing trichoderma of fermenting
Fertilizer；
(2) spore quantity is reached 1010The bacillus amyloliquefaciens SQR9 liquid spawn of a/mL is added to institute by 0.2% amount
It in the turf biological organic fertilizer containing trichoderma stated, mixes well, control moisture is lower than 30%, obtains and conciliates starch gemma containing trichoderma
The turf biological organic fertilizer of bacillus；
Method three includes the following steps
(1) suitable quantity of water is added into turf and alkaline matter adjusts turf pH to 6.5~7.5, carbon source is added, it is dilute that amino acid is added
It releases liquid and adjusts water content to 40%~50%, access the bacillus amyloliquefaciens (Bacillus that deposit number is CGMCC NO.5808
Amyloliquefaciens aerobic compost) is carried out after SQR9 seed liquor, is fermented 7~20 days and is obtained containing bacillus amyloliquefaciens
Turf biological organic fertilizer；
(2) Guizhou trichoderma NJAU4742 direct fermentation crop material is utilized to prepare according to method disclosed in 201610151640.1
Trichoderma solid spawn, trichoderma concentration is 10 in prepared trichoderma solid spawn8cfu/ml；
(3) trichoderma solid spawn prepared by step (2) is added to the turf biology containing bacillus amyloliquefaciens by 5% amount has
It in machine fertilizer, mixes well, control moisture is lower than 30%, obtains the turf biological organic fertilizer containing trichoderma and bacillus amyloliquefaciens.
2. preparation method according to claim 1, it is characterised in that the turf biological organic fertilizer preparation containing trichoderma
Method includes: that suitable quantity of water and alkaline matter adjustment turf pH to 4.5~6.5 are added into turf, is added thereto after pH stablizes
Enter carbon source, amino acid dilution is added and adjusts water content to 40%~50%, mixes, stands 20~24 hours, deposit number is added
For Guizhou trichoderma (Trichoderma guizhouense) NJAU4742 fermentation liquid of CGMCC NO.12166, after mixing well
It stacks into pile and carries out aerobic compost, ferment 7~20 days turf biological organic fertilizers of the acquisition containing trichoderma；The wherein carbon source
Additional amount is adjust the turf quality after pH 5%~10%；Effective bacterium number amount reaches in the NJAU4742 fermentation liquid
109CFU·mL-1, the additive amount of NJAU4742 fermentation liquid is adjust the turf weight after pH 5%~15%.
3. preparation method according to claim 1, it is characterised in that the turf biology containing bacillus amyloliquefaciens
Preparation method of organic fertilizer includes: that suitable quantity of water and alkaline matter adjusting turf pH to 6.5~7.5 are added into turf, is stablized to pH
Carbon source is added thereto afterwards, amino acid dilution is added and adjusts water content to 40%~50%, stands 20~24 hours, is added and protects
Hiding number is bacillus amyloliquefaciens (Bacillus amyloliquefaciens) SQR9 seed liquor of CGMCC NO.5808, is filled
Divide to stack after mixing and carries out an aerobic compost at pile, 7~20 days turf biological organics of the acquisition containing bacillus amyloliquefaciens of fermenting
Fertilizer；Wherein the additional amount of the carbon source is adjust the turf quality after pH 5%~10%；Effective bacterium number in SQR9 seed liquor
Amount reaches 109CFU·mL-1, the additive amount of SQR9 seed liquor is adjust the turf weight after pH 5%~15%.
4. preparation method according to claim 1, it is characterised in that the bacillus amyloliquefaciens SQR9 liquid spawn
It is prepared by the following method to obtain: the bacillus amyloliquefaciens SQR9 strain that will be stored in -80 DEG C of refrigerator glycerol tubes, it is solid in LB
It crosses and activates on body culture medium, be placed in overnight incubation in 37 DEG C of incubators, inoculation SQR9 single bacterium drops down onto LB liquid medium, so
It is placed on 30 DEG C, shakes 12h on 170rpm shaking table, then be inoculated into after LB liquid medium by 0.1% (v/v) and to shake 36h and obtain
SQR9 fermentation liquid, effective bacterium number amount reach 109CFU·mL-1, spare.
5. preparation method according to any one of claims 1 to 4, it is characterised in that the alkaline matter is vegetation
Ash.
6. preparation method according to any one of claims 1 to 4, it is characterised in that water is added when adjusting pH to turf heap
Body water content is 30%~40%.
7. preparation method according to claims 1 to 4, which is characterized in that the carbon source is rice bran or powdered rice hulls or mushroom
Ground-slag.
8. preparation method according to claims 1 to 4, which is characterized in that the amino acid dilution is to be diluted with water
Full price aminoacids solution, the additional amount of full price aminoacids solution be adjust pH after turf weight 3%~10%.
9. preparation method according to claim 1, which is characterized in that work as when aerobic compost heap temperature rise to 50 DEG C of progress
First time turning；During composting, turning is carried out when heap temperature no longer rises.
10. preparation method according to claim 1, it is characterised in that a length of 7~15 days when the compost of spring or summer；Autumn
A length of 15~20 days when the compost in season or winter.
11. the turf biological organic fertilizer prepared according to method according to any one of claims 1 to 4.
12. turf biological organic fertilizer described in claim 11 is promoting plant growth and/or is improving the application in crop quality.
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