CN104744182A - Tobacco water retention biological organic fertilizer and preparation method thereof - Google Patents
Tobacco water retention biological organic fertilizer and preparation method thereof Download PDFInfo
- Publication number
- CN104744182A CN104744182A CN201510031541.5A CN201510031541A CN104744182A CN 104744182 A CN104744182 A CN 104744182A CN 201510031541 A CN201510031541 A CN 201510031541A CN 104744182 A CN104744182 A CN 104744182A
- Authority
- CN
- China
- Prior art keywords
- organic fertilizer
- biological organic
- tobacco
- fermentation
- content
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 45
- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 244000061176 Nicotiana tabacum Species 0.000 title abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 66
- 238000000855 fermentation Methods 0.000 claims abstract description 41
- 230000004151 fermentation Effects 0.000 claims abstract description 41
- 241000208125 Nicotiana Species 0.000 claims abstract description 39
- 239000002361 compost Substances 0.000 claims abstract description 21
- 239000000052 vinegar Substances 0.000 claims abstract description 21
- 235000021419 vinegar Nutrition 0.000 claims abstract description 21
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 15
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000004021 humic acid Substances 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims description 21
- 239000000758 substrate Substances 0.000 claims description 21
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 claims description 12
- 235000013923 monosodium glutamate Nutrition 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 11
- 239000004223 monosodium glutamate Substances 0.000 claims description 9
- 229920002643 polyglutamic acid Polymers 0.000 claims description 9
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 8
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 7
- 235000013379 molasses Nutrition 0.000 claims description 7
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 6
- 238000011218 seed culture Methods 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 6
- 230000035784 germination Effects 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- 239000005416 organic matter Substances 0.000 claims description 5
- 239000002893 slag Substances 0.000 claims description 5
- 239000011159 matrix material Substances 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 241000726221 Gemma Species 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 230000001332 colony forming effect Effects 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000012262 fermentative production Methods 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 230000028327 secretion Effects 0.000 claims description 3
- 229940073490 sodium glutamate Drugs 0.000 claims description 3
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- 239000000047 product Substances 0.000 claims description 2
- 239000002689 soil Substances 0.000 abstract description 18
- 239000000463 material Substances 0.000 abstract description 9
- 239000002699 waste material Substances 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 239000002028 Biomass Substances 0.000 abstract 2
- 238000013329 compounding Methods 0.000 abstract 1
- 235000019504 cigarettes Nutrition 0.000 description 14
- 239000003337 fertilizer Substances 0.000 description 12
- 235000013339 cereals Nutrition 0.000 description 11
- 238000000034 method Methods 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 241000193830 Bacillus <bacterium> Species 0.000 description 4
- 241000209094 Oryza Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
- 210000003608 fece Anatomy 0.000 description 4
- 239000010903 husk Substances 0.000 description 4
- 235000009566 rice Nutrition 0.000 description 4
- 231100000252 nontoxic Toxicity 0.000 description 3
- 230000003000 nontoxic effect Effects 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 241000287828 Gallus gallus Species 0.000 description 2
- 238000009264 composting Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000002440 industrial waste Substances 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 239000002068 microbial inoculum Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 108010020346 Polyglutamic Acid Proteins 0.000 description 1
- 108010039918 Polylysine Proteins 0.000 description 1
- 241001002356 Valeriana edulis Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000002362 mulch Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000656 polylysine Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 239000000230 xanthan gum Substances 0.000 description 1
- 229940082509 xanthan gum Drugs 0.000 description 1
- 235000010493 xanthan gum Nutrition 0.000 description 1
Landscapes
- Fertilizers (AREA)
Abstract
The invention discloses a tobacco water retention biological organic fertilizer and a preparation method thereof. The preparation method comprises the following steps: fermenting wastes by using bacillus amyloliquefaciens (IAE), compounding the fermentation product with humic acid, and then secondarily fermenting vinegar residue compost to from a biological organic fertilizer which is suitable for tobaccos and has a water retention effect. In the biological organic fertilizer, the number of effective strains is more than or equal to 0.5*10<8>cfu/g, the content of an effective water retention material is more than or equal to 21g/kg in dry weight, the water content is less than or equal to 30%, and the organic content is more than or equal to 45%. A test shows that after the tobacco is transplanted for 30 days, the water content of 0-10cm of the tobacco field soil in which the biological organic fertilizer is applied is 32.7% higher than that of the soil in which the biological organic fertilizer is not applied and the tobacco plant biomass is increased by 55.6%. The water retention biological organic fertilizer disclosed by the invention can significantly increase the water content of the soil and improve the biomass of tobacco plants at the seedling stage.
Description
Technical field
The invention belongs to conservation technology field, relate to one and to grow tobacco water conservation biological organic fertilizer and preparation method thereof.
Background technology
China is the country that tobacco cultivation area is maximum in the world, the millions of mu of part province tobacco planting area, and tobacco income is an important sources of local farmers family income.The plantation time of the tobacco of some areas is 2 annual ~ May, is the time period that in 1 year, precipitation is less, and the tobacco of plantation needs pouring of manually carrying water, the manpower of at substantial.
Water keeping fertilizer is the fertilizer that a class contains water conservation material, and the water conservation material wherein added in most of water keeping fertilizer is polyacrylamide substances or humic acid material.The chemical substance of part easily causes rotten of soil compaction, plant, and partial chemical material decomposition after product has bio-toxicity.Microbial secondary meta-bolites, if polyamino acid, macromolecule polysaccharide are as polyglutamic acid, poly-lysine, xanthan gum etc., has very strong water-absorbing-retaining effect, and nontoxic.Sterling that produced by fermentable, that have water retention is widely used in the high added value industries such as food, medicine, makeup, directly adds the fertilizer being formed in fertilizer and have water retaining function to, rarely has report.
Tobacco is mainly based on ridge cultivation, and overlay film insulation soil moisture conservation on ridge, through hole-ground film on ridge, drops into cigarette seedling in hole, to be watered final singling to tobacco root by water ladle.Rainfall can rely on the water of mulch film surrounding to concentrate to Tobacco Root base portion, then infiltrates foundation soil, and tobacco fertility moisture is in earlier stage most important for surviving of cigarette seedling.Use water conservation biological organic fertilizer the normal root water of manual watering and natural precipitation can be immobilizated in cigarette shoot root portion, be conducive to cigarette seedling and survive, improve the utilising efficiency of moisture.
As can be seen here, invent and be a kind ofly applicable to ridge to plant the water conservation biological organic fertilizer of crop tobacco very necessary.
Summary of the invention
Goal of the invention: for solving problems of the prior art, the invention provides a water conservation biological organic fertilizer grown tobacco, this biological organic fertilizer is produced by fermentable waste, can improve the soil moisture that ridge plants crop tobacco.
Technical scheme: for realizing above-mentioned technical purpose, the present invention proposes one and to grow tobacco water conservation biological organic fertilizer, described tobacco water conservation biological organic fertilizer is by bacillus amyloliquefaciens IAE (the Bacillus.amyloliquefaciens IAE with secretion γ-polyglutamic acid function, on March 15th, 2013 is preserved in China typical culture collection center, preservation address is Wuhan, China Wuhan University, culture presevation number is CCTCC NO:M 2013086) obtain through fermenting twice preparation, wherein, described tobacco water conservation biological organic fertilizer contains 0.5 × 10
8cfu g
-1above bacillus amyloliquefaciens IAE, γ-polyglutamic acid content>=21g kg
-1dry weight, water content≤30%, organic content>=45%.
Particularly, the fermentation substrate of fermenting twice is respectively one grade fermemtation substrate and second order fermentation substrate, and wherein, described one grade fermemtation substrate comprises the component of following parts by weight: residue of monosodium glutamate 150 ~ 200 parts, bean cake powder 70 ~ 90 parts, molasses slag 20 ~ 30 parts and MgSO
40.5 part is prepared from; Described second order fermentation substrate comprises the component of following parts by weight: humic acid 10 ~ 15 parts and vinegar grain 20 ~ 30 parts, compost.
Preferably, described vinegar grain compost is the vinegar grain compost after becoming thoroughly decomposed, and germination index more than 98%, quality of organic matter are 30% ~ 35% than content >=45%, water content mass ratio.
Wherein, after each component mixing of described one grade fermemtation substrate, water content is regulated to be for subsequent use after 50 ~ 55%, 115 DEG C of sterilizing 30min.
Present invention further proposes the preparation method of above-mentioned tobacco water conservation biological organic fertilizer, comprise the steps:
(1) bacillus amyloliquefaciens IAE is inoculated into liquid seed culture medium to cultivate, culture condition is: culture temperature 37 DEG C, shaking speed 130 ~ 160rpm, and incubation time 24 hours, seed bacteria containing amount is greater than 1 × 10
9cfu ml
-1then by seed according to 5% volume ratio be inoculated in liquid fermentation medium and carry out liquid fermenting production, the condition of fermentative production is: culture temperature 28 ~ 35 DEG C, dissolved oxygen air flow scope is 30 ~ 100%, 120 ~ 160rpm, fermentation latter portions bacterial strain forms gemma, wherein colony-forming unit>=1 × 10 of bacterial strain
9cfu ml
-1, obtain the fermented liquid of bacillus amyloliquefaciens IAE;
(2) by residue of monosodium glutamate 150 ~ 200g, bean cake powder 70 ~ 90g, molasses slag 20 ~ 30g and MgSO
40.5g mixes, regulate water content 50 ~ 55wt%, sterilizing 30min at 115 DEG C, obtain one-level solid fermentation matrix, the fermented liquid to bacillus amyloliquefaciens IAE that the ratio inoculation step (1) of amass according to one-level solid fermentation matrix body 5% obtains, above-mentioned fermented substrate is piled the bar buttress of 1 ~ 2 meter wide, 0.8 ~ 1.2 meter high, turning is started when bar buttress core temperature 55 ~ 60 DEG C, according to the difference of outside temperature, ferment 15 ~ 20 days, obtain one time fermentation product;
(3) by the one time fermentation product of step (2) gained and humic acid mixing, leave standstill and stablize 3 ~ 5h, leave standstill the solid substance after stablizing and the mixing of the grain of the vinegar after becoming thoroughly decomposed compost, Secondary Fermentation 3 ~ 5 days, obtain Secondary Fermentation product, wherein, the ratio of weight and number 50 ~ 55: 10 ~ 15: 40 ~ 50 of one time fermentation product, humic acid and vinegar grain compost;
(4) by the Secondary Fermentation after product cryodrying of step (3) gained to water content mass ratio≤30%, pack and obtain tobacco water conservation biological organic fertilizer.
Particularly, in step (1), the formula of described liquid seed culture medium is, according to 1L substratum: extractum carnis 3g, peptone 10g, NaCl 5g, for subsequent use after tap water 1000ml, pH scope 7.0 ~ 7.5,121 DEG C of sterilizing 20min.
In step (1), liquid fermentation medium formula used is, prepares: glucose 80g, bean powder 30g, Sodium Glutamate 2g, (NH according to 1L substratum
4)
2sO
46g, K
2hPO
42g, MgSO
40.25g, MnSO
40.2g, distilled water 1000ml, pH value nature, for subsequent use after 115 DEG C of sterilizing 30min.
Preferably, step (3) is used become thoroughly decomposed after vinegar grain compost be germination index more than 98%, quality of organic matter than content >=45%, water content mass ratio 30% ~ 35% vinegar grain compost.
Bacillus amyloliquefaciens IAE (Bacillus.amyloliquefaciens IAE), on March 15th, 2013 is preserved in China typical culture collection center, preservation address is Wuhan, China Wuhan University, and culture presevation number is CCTCC NO:M 2013086.
Beneficial effect: compared with prior art, tool of the present invention has the following advantages:
(1) the present invention adopts tankage residue of monosodium glutamate and the sugar industry industrial waste molasses the like waste of microorganism strains fermentation monosodium glutamate manufacture, and no matter be fermentation substrate, or tunning is all nontoxic, the cultivation being applicable to tobacco uses;
(2) in the present invention, Secondary Fermentation substrate adopts vinegar grain compost, fermenting process due to vinegar adds the air permeability that a large amount of rice husks increases fermented substrate, in this composting material, rice husk content is higher, product of the present invention can increase the hole of soil, compared to compost such as the cow dung become thoroughly decomposed, chicken manures, be more conducive to γ-polyglutamic acid etc. effectively water conservation material moisture stored be held in interstices of soil.
Accompanying drawing explanation
The schematic diagram of 0 ~ 10cm soil moisture content below the strain of Fig. 1 different treatment cigarette;
The schematic diagram of Fig. 2 different treatment cigarette strain fresh weight.
Embodiment
The present invention proposes one and to grow tobacco water conservation biological organic fertilizer, this tobacco water conservation biological organic fertilizer is by bacillus amyloliquefaciens IAE (the Bacillus.amyloliquefaciens IAE with secretion γ-polyglutamic acid function, on March 15th, 2013 is preserved in China typical culture collection center, preservation address is Wuhan, China Wuhan University, culture presevation number is CCTCC NO:M 2013086) obtain through fermenting twice preparation, wherein, this tobacco water conservation biological organic fertilizer contains 0.5 × 10
8cfu g
-1above bacillus amyloliquefaciens IAE, γ-polyglutamic acid content>=21g kg
-1dry weight, water content≤30%, organic content>=45%.Specifically will set forth its preparation method below.
The preparation of embodiment 1 tobacco water conservation biological organic fertilizer.
(1) microbial inoculum is produced
1) Bacillus.amyloliquefaciens IAE is inoculated into liquid seed culture medium to cultivate, culture condition is: culture temperature 37 DEG C, shaking speed 130 ~ 160rpm, and incubation time 24 hours, seed bacteria containing amount is greater than 1 × 10
9cfu ml
-1seed is inoculated into fermentor tank according to 5% volume ratio and carries out liquid fermenting production, the condition of its fermentative production is: culture temperature 28 ~ 35 DEG C, dissolved oxygen air flow scope is 30 ~ 100%, 120 ~ 160rpm, fermentation latter portions bacterial strain forms gemma, colony-forming unit>=1 × 10 of this bacterial strain of fermented liquid
9cfu ml
-1, the retrogradation of fermented liquid concentration; The formula of the seed culture medium of liquid used is, prepares: extractum carnis 3g according to 1L substratum, peptone 10g, NaCl 5g, tap water 1000ml, pH scope 7.0-7.5,121 DEG C of sterilizing 20min.Liquid fermentation medium formula used is, prepares: glucose 80g, bean powder 30g, Sodium Glutamate 2g, (NH according to 1L substratum
4)
2sO
46g, NaCl 5g, K
2hPO
42g, MgSO
40.25g, MnSO
40.2g, distilled water 1000ml, pH value nature, 115 DEG C of sterilizing 30min;
2) by residue of monosodium glutamate 150 ~ 200g, bean cake powder 70 ~ 90g, molasses slag 20 ~ 30g and MgSO
40.5g, mixing, regulate water content 50 ~ 55wt%, 115 DEG C of sterilizing 30min, according to the volume ratio inoculation B.amyloliquefaciens IAE fermented liquid of 5%, fermented substrate is piled the bar buttress of 1 ~ 2 meter wide, 0.8 ~ 1.2 meter high, when bar buttress core temperature 55 ~ 60 DEG C, start turning, according to the difference of outside temperature, ferment 15 ~ 20 days.
(2) solid fermentation
1) by step 2 that microbial inoculum is produced) tunning 50 ~ 55 parts (parts by weight, lower with) and humic acid 10 ~ 15 parts mix thoroughly, standingly stablize 3 ~ 5h.Choose germination index more than 98%, quality of organic matter than content >=45%, water content mass ratio 30% ~ 35%, become thoroughly decomposed vinegar grain compost, by all leave standstill stable after solid substance and vinegar grain 40 ~ 50 parts, compost mix thoroughly, Secondary Fermentation 3 ~ 5 days, obtains Secondary Fermentation product.
2) by the cryodrying of Secondary Fermentation product to water content mass ratio≤30%, packaging is dispatched from the factory and is tobacco water conservation biological organic fertilizer.
The potted plant experiment effect of embodiment 2 tobacco water conservation biological organic fertilizer.
Test selects tobacco bred to be K326.The method for planting of tobacco is pass hole-ground film on ridge, and cigarette seedling is put into hole, then to cigarette shoot root border pouring normal root water.Water conservation biological organic fertilizer application process is, after tobacco drops into hole, by the hole on film, adds the water conservation biological organic fertilizer of the common fertilizer of 60g or embodiment 1 preparation to every strain cigarette shoot root border, then waters 300ml normal root water.
Setup Experiments three process, is respectively:
(1) contrast, do not use common fertilizer or water conservation biological organic fertilizer;
(2) common fertilizer, uses common fertilizer;
(3) water retention organic fertilizer (prepared by embodiment 1), uses water conservation biological organic fertilizer.
Cigarette transplantation of seedlings is after 30 days, measures 0 ~ 10cm soil moisture content immediately below cigarette strain and measures the fresh weight of cigarette strain.
The fresh weight measuring 0 ~ 10cm soil moisture content and tobacco plant respectively as depicted in figs. 1 and 2, uses common fertilizer and biological water-keeping fertilizer can both increase the water content of 0 ~ 10cm soil and the fresh weight of tobacco plant significantly.Compared to control treatment, fertilizer process soil moisture content and plant fresh weight add 5.2% and 8.9% respectively, and water retention organic fertilizer process soil moisture content and plant fresh weight add 32.7% and 55.6% respectively.Water conservation biological organic fertilizer can increase the water content of cigarette strain rhizosphere soil and the fresh weight of cigarette strain in seedling stage significantly.
The present invention is by adopting tankage residue of monosodium glutamate and the sugar industry industrial waste molasses the like waste of microorganism strains fermentation monosodium glutamate manufacture, and no matter be fermentation substrate, or tunning is all nontoxic, the cultivation being applicable to tobacco uses; And Secondary Fermentation substrate adopts vinegar grain compost, fermenting process due to vinegar adds the air permeability that a large amount of rice husks increases fermented substrate, in this composting material, rice husk content is higher, therefore, product of the present invention can increase the hole of soil, compared to compost such as the cow dung become thoroughly decomposed, chicken manures, be more conducive to γ-polyglutamic acid etc. effectively water conservation material moisture stored be held in interstices of soil.
Claims (8)
1. one to grow tobacco water conservation biological organic fertilizer, it is characterized in that, described tobacco water conservation biological organic fertilizer is prepared through fermenting twice by the bacillus amyloliquefaciens IAE with secretion γ-polyglutamic acid function and obtains, and wherein, described tobacco water conservation biological organic fertilizer contains 0.5 × 10
8cfu g
-1above bacillus amyloliquefaciens IAE, γ-polyglutamic acid content>=21g kg
-1dry weight, water content≤30%, organic content>=45%.
2. tobacco water conservation biological organic fertilizer according to claim 1, it is characterized in that, the fermentation substrate of fermenting twice is respectively one grade fermemtation substrate and second order fermentation substrate, wherein, described one grade fermemtation substrate comprises the component of following parts by weight: residue of monosodium glutamate 150 ~ 200 parts, bean cake powder 70 ~ 90 parts, molasses slag 20 ~ 30 parts and MgSO
40.5 part is prepared from; Described second order fermentation substrate comprises the component of following parts by weight: humic acid 10 ~ 15 parts and vinegar grain 20 ~ 30 parts, compost.
3. tobacco water conservation biological organic fertilizer according to claim 2, is characterized in that, described vinegar grain compost is the vinegar grain compost after becoming thoroughly decomposed, and germination index more than 98%, quality of organic matter are 30% ~ 35% than content >=45%, water content mass ratio.
4. according to claim 2 said tobacco water conservation biological organic fertilizer, it is characterized in that, after each component mixing of described one grade fermemtation substrate, regulate water content to be for subsequent use after 50 ~ 55%, 115 DEG C of sterilizing 30min.
5. the preparation method of tobacco water conservation biological organic fertilizer according to claim 1, is characterized in that, comprise the steps:
(1) bacillus amyloliquefaciens IAE is inoculated into liquid seed culture medium to cultivate, culture condition is: culture temperature 37 DEG C, shaking speed 130 ~ 160rpm, and incubation time 24 hours, seed bacteria containing amount is greater than 1 × 10
9cfuml
-1then by seed according to 5% volume ratio be inoculated in liquid fermentation medium and carry out liquid fermenting production, the condition of fermentative production is: culture temperature 28 ~ 35 DEG C, dissolved oxygen air flow scope is 30 ~ 100%, 120 ~ 160rpm, fermentation latter portions bacterial strain forms gemma, wherein colony-forming unit>=1 × 10 of bacterial strain
9cfuml
-1, obtain the fermented liquid of bacillus amyloliquefaciens IAE;
(2) by residue of monosodium glutamate 150 ~ 200g, bean cake powder 70 ~ 90g, molasses slag 20 ~ 30g and MgSO
40.5g mixes, regulate water content 50 ~ 55wt%, sterilizing 30min at 115 DEG C, obtain one-level solid fermentation matrix, the fermented liquid to bacillus amyloliquefaciens IAE that the ratio inoculation step (1) of amass according to one-level solid fermentation matrix body 5% obtains, above-mentioned fermented substrate is piled the bar buttress of 1 ~ 2 meter wide, 0.8 ~ 1.2 meter high, turning is started when bar buttress core temperature 55 ~ 60 DEG C, according to the difference of outside temperature, ferment 15 ~ 20 days, obtain one time fermentation product;
(3) by the one time fermentation product of step (2) gained and humic acid mixing, leave standstill and stablize 3 ~ 5h, leave standstill the solid substance after stablizing and the mixing of the grain of the vinegar after becoming thoroughly decomposed compost, Secondary Fermentation 3 ~ 5 days, obtain Secondary Fermentation product, wherein, the ratio of weight and number 50 ~ 55: 10 ~ 15: 40 ~ 50 of one time fermentation product, humic acid and vinegar grain compost;
(4) by the Secondary Fermentation after product cryodrying of step (3) gained to water content mass ratio≤30%, pack and obtain tobacco water conservation biological organic fertilizer.
6. preparation method according to claim 5, it is characterized in that, in step (1), the formula of described liquid seed culture medium is, according to 1L substratum: extractum carnis 3g, peptone 10g, NaCl 5g, for subsequent use after tap water 1000ml, pH scope 7.0 ~ 7.5,121 DEG C of sterilizing 20min.
7. preparation method according to claim 5, is characterized in that, in step (1), liquid fermentation medium formula used is, prepares: glucose 80g, bean powder 30g, Sodium Glutamate 2g, (NH according to 1L substratum
4)
2sO
46g, K
2hPO
42g, MgSO
40.25g, MnSO
40.2g, distilled water 1000ml, pH value nature, for subsequent use after 115 DEG C of sterilizing 30min.
8. preparation method according to claim 5, is characterized in that, step (3) is used become thoroughly decomposed after vinegar grain compost be germination index more than 98%, quality of organic matter than content >=45%, water content mass ratio 30% ~ 35% vinegar grain compost.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510031541.5A CN104744182B (en) | 2015-01-21 | 2015-01-21 | One grows tobacco water conservation biological organic fertilizer and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510031541.5A CN104744182B (en) | 2015-01-21 | 2015-01-21 | One grows tobacco water conservation biological organic fertilizer and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104744182A true CN104744182A (en) | 2015-07-01 |
| CN104744182B CN104744182B (en) | 2017-07-18 |
Family
ID=53584545
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510031541.5A Active CN104744182B (en) | 2015-01-21 | 2015-01-21 | One grows tobacco water conservation biological organic fertilizer and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104744182B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105295934A (en) * | 2015-10-26 | 2016-02-03 | 富川富兴果蔬有限责任公司 | Novel water-retaining agent using vinegar factory liquid waste and preparation method thereof |
| CN105542778A (en) * | 2015-12-16 | 2016-05-04 | 罗永城 | Humic acid type organic water-retaining agent and preparation method thereof |
| CN105542783A (en) * | 2015-12-16 | 2016-05-04 | 罗永城 | Humic acid type inorganic water-retaining agent by using edible vinegar factory waste liquid and preparation method thereof |
| CN105542777A (en) * | 2015-12-16 | 2016-05-04 | 罗永城 | Humic acid type water-retaining agent by using edible vinegar factory waste liquid and preparation method thereof |
| CN105542780A (en) * | 2015-12-16 | 2016-05-04 | 罗永城 | Novel water-retaining agent by using edible vinegar factory waste liquid and preparation method thereof |
| CN105567243A (en) * | 2015-12-16 | 2016-05-11 | 罗永城 | Humic acid-type inorganic-organic water-retaining agent utilizing waste liquid of vinegar factory and preparation method for humic acid-type inorganic-organic water-retaining agent |
| CN106518350A (en) * | 2016-10-27 | 2017-03-22 | 六安市振清生态农业有限公司 | Nutritive organic matrix for taxus chinensis planting |
| CN107058151A (en) * | 2016-11-29 | 2017-08-18 | 广东森度生态农业科技有限公司 | A kind of microbial bacteria with fermentation water retaining function and its application |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1648107A (en) * | 2005-01-10 | 2005-08-03 | 左淑珍 | Biological organic fertilizer and its preparing method |
| CN101786916A (en) * | 2010-03-23 | 2010-07-28 | 山西晨雨科技开发连锁经营有限公司 | Bio-fertilizer produced by fermenting steel slag, vinegar residue and weathered coal through microbes and preparation method thereof |
| CN102863288A (en) * | 2012-09-28 | 2013-01-09 | 中国农业科学院烟草研究所青岛科技开发中心 | Organic-inorganic compound formula for tobacco |
| CN103172437A (en) * | 2012-12-31 | 2013-06-26 | 天津北洋百川生物技术有限公司 | Liquid compound microbial fertilizer containing polyglutamic acid, and preparation method and application thereof |
| CN103804067A (en) * | 2013-07-22 | 2014-05-21 | 河海大学 | Biological agent for supporting calcium to facility plantation crops as well as preparation method and application of biological agent |
| CN103805204A (en) * | 2013-07-22 | 2014-05-21 | 河海大学 | Farmland soil microorganism water-holding product and preparation method thereof |
-
2015
- 2015-01-21 CN CN201510031541.5A patent/CN104744182B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1648107A (en) * | 2005-01-10 | 2005-08-03 | 左淑珍 | Biological organic fertilizer and its preparing method |
| CN101786916A (en) * | 2010-03-23 | 2010-07-28 | 山西晨雨科技开发连锁经营有限公司 | Bio-fertilizer produced by fermenting steel slag, vinegar residue and weathered coal through microbes and preparation method thereof |
| CN102863288A (en) * | 2012-09-28 | 2013-01-09 | 中国农业科学院烟草研究所青岛科技开发中心 | Organic-inorganic compound formula for tobacco |
| CN103172437A (en) * | 2012-12-31 | 2013-06-26 | 天津北洋百川生物技术有限公司 | Liquid compound microbial fertilizer containing polyglutamic acid, and preparation method and application thereof |
| CN103804067A (en) * | 2013-07-22 | 2014-05-21 | 河海大学 | Biological agent for supporting calcium to facility plantation crops as well as preparation method and application of biological agent |
| CN103805204A (en) * | 2013-07-22 | 2014-05-21 | 河海大学 | Farmland soil microorganism water-holding product and preparation method thereof |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105295934A (en) * | 2015-10-26 | 2016-02-03 | 富川富兴果蔬有限责任公司 | Novel water-retaining agent using vinegar factory liquid waste and preparation method thereof |
| CN105542778A (en) * | 2015-12-16 | 2016-05-04 | 罗永城 | Humic acid type organic water-retaining agent and preparation method thereof |
| CN105542783A (en) * | 2015-12-16 | 2016-05-04 | 罗永城 | Humic acid type inorganic water-retaining agent by using edible vinegar factory waste liquid and preparation method thereof |
| CN105542777A (en) * | 2015-12-16 | 2016-05-04 | 罗永城 | Humic acid type water-retaining agent by using edible vinegar factory waste liquid and preparation method thereof |
| CN105542780A (en) * | 2015-12-16 | 2016-05-04 | 罗永城 | Novel water-retaining agent by using edible vinegar factory waste liquid and preparation method thereof |
| CN105567243A (en) * | 2015-12-16 | 2016-05-11 | 罗永城 | Humic acid-type inorganic-organic water-retaining agent utilizing waste liquid of vinegar factory and preparation method for humic acid-type inorganic-organic water-retaining agent |
| CN106518350A (en) * | 2016-10-27 | 2017-03-22 | 六安市振清生态农业有限公司 | Nutritive organic matrix for taxus chinensis planting |
| CN107058151A (en) * | 2016-11-29 | 2017-08-18 | 广东森度生态农业科技有限公司 | A kind of microbial bacteria with fermentation water retaining function and its application |
| CN107058151B (en) * | 2016-11-29 | 2021-08-20 | 广东森度生态农业科技有限公司 | Microbial bacterium with fermentation water retention function and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104744182B (en) | 2017-07-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103964946B (en) | A kind of paddy rice Special compound microbial fertilizer and preparation method thereof | |
| CN104744182B (en) | One grows tobacco water conservation biological organic fertilizer and preparation method thereof | |
| CN102965319B (en) | Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof | |
| CN102863265A (en) | Flower planting nutrient soil and production method thereof | |
| CN107652077A (en) | A kind of agricultural ferment production method and application thereof of mass, serialization | |
| CN110330370A (en) | While a kind of turf biological organic fertilizer and its preparation method and application containing trichoderma and bacillus amyloliquefaciens | |
| CN109369228A (en) | A kind of method that can promote the decomposed speed of solid organic fertilizer | |
| CN104447054A (en) | Special biological organic fertilizer for tea | |
| CN105315080B (en) | A method of utilizing life excreta and stalk fermentation biological organic fertilizer | |
| CN106591147A (en) | Aspergillus niger NJDL-12 bacterial strain and application thereof to improvement of coastal saline-alkali soil | |
| CN111919709A (en) | Cow dung basic carrier culture medium containing plant growth-promoting bacteria and preparation method thereof | |
| CN1254696A (en) | Compound biological fertilizer | |
| CN102942420B (en) | Special microbial agent and bio-organic fertilizer for sweet cherry and preparation methods of special microbial agent | |
| CN103664251A (en) | Bio-fertilizer produced by using agricultural wastes | |
| CN106854108A (en) | A kind of preparation method of burdock fertilizer special for organic | |
| CN114702357B (en) | High-activity compound microbial fertilizer and preparation method thereof | |
| CN110591937B (en) | Antagonistic actinomycetes and biological organic fertilizer for preventing and controlling tomato bacterial wilt, method and application | |
| CN105724055B (en) | A method of improving agaricus bisporus yield using needle mushroom dreg | |
| CN103951479B (en) | Fertilizer and preparation method thereof | |
| CN108383653A (en) | A kind of method and bio-organic fertilizer preparing fertilizer using stalk | |
| CN108456651A (en) | A kind of composite bacteria agent of fermented stalk and preparation method thereof | |
| CN112745151A (en) | Composite biological organic fertilizer containing bacillus and trichoderma and preparation method thereof | |
| CN103964928A (en) | Preparation method of microorganism soil restoration agent | |
| CN107963922A (en) | A kind of preparation method of Enteromorpha ferment organic fertilizer | |
| CN107602278A (en) | The preparation method of tobacco breeding and seedling nursing with equipment matrix |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231127 Address after: 201415 No. 3258, Nanting Road, Fengxian District, Shanghai Patentee after: Shanghai Xikun Biotechnology Co.,Ltd. Address before: No. 5-18-1, Hope City Commercial Street, No. 477 Lushan North Road, Jingyang District, Deyang City, Sichuan Province, 618000 Patentee before: Sichuan Wanhe Intellectual Property Operation Co.,Ltd. Effective date of registration: 20231127 Address after: No. 5-18-1, Hope City Commercial Street, No. 477 Lushan North Road, Jingyang District, Deyang City, Sichuan Province, 618000 Patentee after: Sichuan Wanhe Intellectual Property Operation Co.,Ltd. Address before: No. 8, West Road, Buddha city, Jiangning District, Nanjing, Jiangsu Patentee before: HOHAI University |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240116 Address after: No.58 Shangmao Street, Jiangzhou Town, Huidong County, Liangshan Yi Autonomous Prefecture, Sichuan Province, 615000 Patentee after: Huidong Yinong Technology Co.,Ltd. Address before: 201415 No. 3258, Nanting Road, Fengxian District, Shanghai Patentee before: Shanghai Xikun Biotechnology Co.,Ltd. |
|
| TR01 | Transfer of patent right |