CN104744182B - One grows tobacco water conservation biological organic fertilizer and preparation method thereof - Google Patents
One grows tobacco water conservation biological organic fertilizer and preparation method thereof Download PDFInfo
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 67
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 48
- 241000208125 Nicotiana Species 0.000 title claims abstract description 44
- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 41
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 238000000855 fermentation Methods 0.000 claims abstract description 53
- 230000004151 fermentation Effects 0.000 claims abstract description 53
- 239000002361 compost Substances 0.000 claims abstract description 21
- 235000021419 vinegar Nutrition 0.000 claims abstract description 21
- 239000000052 vinegar Substances 0.000 claims abstract description 21
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 14
- 239000005416 organic matter Substances 0.000 claims abstract description 9
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims abstract description 8
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- 239000007788 liquid Substances 0.000 claims description 14
- 239000000047 product Substances 0.000 claims description 12
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- 235000013923 monosodium glutamate Nutrition 0.000 claims description 10
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 9
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 8
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 8
- 229920002643 polyglutamic acid Polymers 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 7
- 239000001963 growth medium Substances 0.000 claims description 7
- 235000013379 molasses Nutrition 0.000 claims description 7
- 239000004223 monosodium glutamate Substances 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 238000011218 seed culture Methods 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- 239000002893 slag Substances 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 230000033228 biological regulation Effects 0.000 claims description 4
- 230000035784 germination Effects 0.000 claims description 4
- 239000011159 matrix material Substances 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 241000726221 Gemma Species 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 108010020346 Polyglutamic Acid Proteins 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 3
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 3
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Abstract
Grown tobacco water conservation biological organic fertilizer and preparation method thereof the invention discloses one.The present invention utilizes bacillus amyloliquefaciens IAE (Bacillus amyloliquefaciens IAE) fermentation wastes, tunning compounds humic acid again, then secondary fermentation vinegar grain compost, forms biological organic fertilizer suitable for tobacco, with Water-saving effect.Effective strain quantity >=0.5 × 10 in the biological organic fertilizer8cfu g‑1, effective water conservation content of material >=21g kg‑1Dry weight, water content≤30%, the content of organic matter >=45%.Experiment display, 30 days after tobacco transplant, soil is not applied using the moisture ratio of biological organic fertilizer vega soil, 0~10cm soil moisture contents are high by 32.7%, cigarette strain biomass increase by 55.6%, the water conservation biological organic fertilizer of the invention can significantly increase the water content of soil and improve the biomass of seedling stage cigarette strain.
Description
Technical field
The invention belongs to conservation technology field, it is related to one and grows tobacco water conservation biological organic fertilizer and preparation method thereof.
Background technology
China is the country of tobacco cultivation area maximum in the world, the millions of mus of part province tobacco planting area, tobacco
Income is an important sources of local farmers family income.The plantation time of the tobacco of some areas is 2 annual~May
Part, it is the precipitation less period in 1 year, the tobacco of plantation needs artificial pouring of carrying water, expends substantial amounts of manpower.
Water keeping fertilizer, is the water conservation material added in the fertilizer that a class contains water conservation material, wherein most water keeping fertilizer
For polyacrylamide substances or humic acid material.Partial chemical substance easily causes rotten of soil hardening, plant, and
And product has bio-toxicity after the decomposition of part chemical substance.Microbial secondary metabolite such as polyamino acid, macromolecule are more
Sugared such as polyglutamic acid, poly-D-lysine, xanthans, with very strong water-absorbing-retaining effect, and it is nontoxic.By microorganism
Fermenting and producing, the sterling with water retention be widely used in the high added value industry such as food, medicine, cosmetics, directly add
It is added in fertilizer and forms the fertilizer with water retaining function, is rarely reported.
Tobacco is main planted with ridge based on, overlay film insulation soil moisture conservation, hole-ground film is passed through on ridge, tobacco seedlings are put into hole on ridge
In, watered final singling to tobacco root by water ladle.Rainfall can be concentrated by the water of mulch film surrounding to Tobacco Root base portion, then be oozed
Enter foundation soil, the moisture of tobacco fertility early stage is most important for surviving for tobacco seedlings.Can be by using water conservation biological organic fertilizer
The root water and natural precipitation of manual watering is immobilizated in tobacco seedlings root, is conducive to tobacco seedlings to survive, and improves the utilization ratio of moisture.
As can be seen here, a kind of water conservation biological organic fertilizer suitable for ridge cultivation crop tobacco is invented very necessary.
The content of the invention
Goal of the invention：To solve problems of the prior art, the present invention provides a water conservation biological organic grown tobacco
Fertilizer, the biological organic fertilizer is produced by microbial fermentation discarded object, it is possible to increase ridge plants the soil moisture of crop tobacco.
Technical scheme：To realize above-mentioned technical purpose, the present invention proposes that one grows tobacco water conservation biological organic fertilizer, the tobacco
Water conservation biological organic fertilizer is by the bacillus amyloliquefaciens IAE with secretion γ-polyglutamic acid function
(Bacillus.amyloliquefaciens IAE, are preserved in China typical culture collection center on March 15th, 2013, protect
Tibetan address is Wuhan, China Wuhan University, and culture presevation number is CCTCC NO：M 2013086) it is prepared through fermenting twice, its
In, the tobacco water conservation biological organic fertilizer contains 0.5 × 108cfu g-1Bacillus amyloliquefaciens IAE, γ above-poly paddy
Histidine content >=21g kg-1Dry weight, water content≤30%, the content of organic matter >=45%.
Specifically, the fermentation substrate of fermenting twice is respectively one grade fermemtation substrate and second order fermentation substrate, wherein, it is described
One grade fermemtation substrate includes the component of following parts by weight：150~200 parts of residue of monosodium glutamate, 70~90 parts of bean cake powder, molasses slag 20~
30 parts and MgSO40.5 part is prepared from；Described second order fermentation substrate includes the component of following parts by weight：Humic acid 10~
15 parts and 20~30 parts of vinegar grain compost.
Preferably, described vinegar grain compost is the vinegar grain compost after becoming thoroughly decomposed, germination index more than 98%, quality of organic matter ratio
Content >=45%, water content mass ratio are 30%~35%.
Wherein, after each component mixing of described one grade fermemtation substrate, regulation water content is 50~55%, 115 DEG C of sterilizings
It is standby after 30min.
Present invention further proposes the preparation method of above-mentioned tobacco water conservation biological organic fertilizer, comprise the following steps：
(1) bacillus amyloliquefaciens IAE is inoculated into liquid seed culture medium culture, condition of culture is：Cultivation temperature 37
DEG C, 130~160rpm of shaking speed, incubation time 24 hours, seed bacteria containing amount is more than 1 × 109cfu ml-1, then by seed
Progress liquid fermentation production in liquid fermentation medium is inoculated into according to 5% volume ratio, the condition of fermenting and producing is：Culture temperature
28~35 DEG C of degree, dissolved oxygen throughput scope is 30~100%, 120~160rpm, and fermentation latter portions bacterial strain forms gemma, its
CFU >=1 × 10 of middle bacterial strain9cfu ml-1, obtain bacillus amyloliquefaciens IAE zymotic fluid；
(2) by 150~200g of residue of monosodium glutamate, 70~90g of bean cake powder, molasses slag 20~30g and MgSO40.5g is mixed, regulation
Water content 50~55wt%, sterilize at 115 DEG C 30min, one-level solid fermentation matrix is obtained, according to one-level solid fermentation matrix body
The zymotic fluid to bacillus amyloliquefaciens IAE that the ratio inoculation step (1) of long-pending 5% is obtained, 1 is piled by above-mentioned fermentation substrate
Start turning when~2 meters wide, 0.8~1.2 meter high bar pile, 55~60 DEG C of bar pile central temperature, according to ambient temperature not
Together, ferment 15~20 days, obtain one time fermentation product；
(3) the one time fermentation product obtained by step (2) and humic acid are mixed, standing stablizes 3~5h, stood after stabilization
Solid content is mixed with the vinegar grain compost after becoming thoroughly decomposed, and secondary fermentation 3~5 days obtains secondary fermentation product, wherein, one time fermentation production
The ratio of weight and number 50~55: 10~15: 40~50 of thing, humic acid and vinegar grain compost；
(4) by product low temperature drying after the secondary fermentation obtained by step (3) to water content mass ratio≤30%, packaging is produced
Tobacco water conservation biological organic fertilizer.
Specifically, in step (1), the formula of described liquid seed culture medium is, according to 1L culture mediums：Beef extract 3g,
It is standby after 7.0~7.5,121 DEG C of sterilizing 20min of peptone 10g, NaCl 5g, running water 1000ml, pH scope.
In step (1), liquid fermentation medium formula used is to be prepared according to 1L culture mediums：Glucose 80g, bean powder
30g, sodium glutamate 2g, (NH4)2SO46g, K2HPO42g, MgSO40.25g, MnSO40.2g, distilled water 1000ml, pH value naturally,
It is standby after 115 DEG C of sterilizing 30min.
Preferably, the vinegar grain compost after being become thoroughly decomposed used by step (3) is germination index more than 98%, quality of organic matter compares content
>=45%, the vinegar grain compost of water content mass ratio 30%~35%.
Bacillus amyloliquefaciens IAE (Bacillus.amyloliquefaciens IAE), on March 15th, 2013 is preserved in
China typical culture collection center, preservation address is Wuhan, China Wuhan University, and culture presevation number is CCTCC NO：M
2013086。
Beneficial effect：Compared with prior art, the invention has the advantages that：
(1) present invention is fermented under the industrial leftover bits and pieces residue of monosodium glutamate of glutamate production and sugar industry industry using microbial strains
Heel molasses the like waste, either fermentation substrate, or tunning are all nontoxic, it is adaptable to which the cultivation of tobacco is used；
(2) secondary fermentation substrate uses vinegar grain compost in the present invention, and adding substantial amounts of rice husk due to the fermentation process of vinegar increases
Plus the aeration of fermentation substrate, rice husk content is higher in the composting material, and product of the invention can increase the hole of soil, phase
Compared with compost such as the cow dungs, chicken manure become thoroughly decomposed, it is more beneficial for the effectively water conservation material such as γ-polyglutamic acid and moisture storage is held in soil
Space.
Brief description of the drawings
The schematic diagram of 0~10cm soil moisture contents below Fig. 1 different disposals cigarette strain；
The schematic diagram of Fig. 2 different disposal cigarette strain fresh weights.
Embodiment
The present invention proposes that one grows tobacco water conservation biological organic fertilizer, and the tobacco water conservation biological organic fertilizer is by with secretion γ-many
Bacillus amyloliquefaciens IAE (the Bacillus.amyloliquefaciens IAE, on March 15th, 2013 of polyglutamic acid function
China typical culture collection center is preserved in, preservation address is Wuhan, China Wuhan University, and culture presevation number is CCTCC NO：
M 2013086) it is prepared through fermenting twice, wherein, this tobacco water conservation biological organic fertilizer contains 0.5 × 108cfu g-1More than
Bacillus amyloliquefaciens IAE, γ-polyglutamic acid content >=21g kg-1Dry weight, water content≤30%, the content of organic matter >=
45%.Its preparation method will be specifically described below.
The preparation of the tobacco water conservation biological organic fertilizer of embodiment 1.
(1) microbial inoculum is produced
1) Bacillus.amyloliquefaciens IAE are inoculated into liquid seed culture medium culture, condition of culture
For：37 DEG C of cultivation temperature, 130~160rpm of shaking speed, incubation time 24 hours, seed bacteria containing amount is more than 1 × 109cfu ml-1, seed is inoculated into fermentation tank according to 5% volume ratio and carries out liquid fermentation production, and the condition of its fermenting and producing is：Cultivation temperature 28
~35 DEG C, dissolved oxygen throughput scope is 30~100%, 120~160rpm, fermentation latter portions bacterial strain formation gemma, zymotic fluid
CFU >=1 × 10 of the bacterial strain9cfu ml-1, zymotic fluid concentration retrogradation；The seed culture medium of liquid used is matched somebody with somebody
Fang Wei, is prepared according to 1L culture mediums：Beef extract 3g, peptone 10g, NaCl 5g, running water 1000ml, pH scope 7.0-7.5,
121 DEG C of sterilizing 20min.Liquid fermentation medium formula used is to be prepared according to 1L culture mediums：Glucose 80g, bean powder 30g,
Sodium glutamate 2g, (NH4)2SO46g, NaCl 5g, K2HPO42g, MgSO40.25g, MnSO40.2g, distilled water 1000ml, pH value
Naturally, 115 DEG C of sterilizing 30min；
2) by 150~200g of residue of monosodium glutamate, 70~90g of bean cake powder, molasses slag 20~30g and MgSO40.5g, is mixed, regulation
Water content 50~55wt%, 115 DEG C of sterilizing 30min, are inoculated with B.amyloliquefaciens IAE according to 5% volume ratio and send out
Zymotic fluid, fermentation substrate is piled 1~2 meter wide, 0.8~1.2 meter high bar pile, starts to turn over when 55~60 DEG C of bar pile central temperature
Heap, according to the difference of ambient temperature, ferments 15~20 days.
(2) solid fermentation
1) step 2 for producing microbial inoculum) 10~15 parts of 50~55 parts of tunning (parts by weight, similarly hereinafter) and humic acid
Thoroughly mix, standing stablizes 3~5h.Germination index more than 98%, quality of organic matter are chosen than content >=45%, water content matter
The vinegar grain compost measure than 30%~35%, become thoroughly decomposed, 40~50 parts of solid content of all standings after stable and vinegar grain compost is thorough
The mixing at bottom, secondary fermentation 3~5 days, obtains secondary fermentation product.
2) by secondary fermentation product low temperature drying to water content mass ratio≤30%, packaging is dispatched from the factory biological as tobacco water conservation
Organic fertilizer.
The potted plant experiment effect of the tobacco water conservation biological organic fertilizer of embodiment 2.
Experiment is K326 from tobacco bred.The method for planting of tobacco is that hole-ground film is passed through on ridge, and tobacco seedlings are put into
Kong Zhong, then pours root water to tobacco seedlings rhizosphere.Water conservation biological organic fertilizer application process is after tobacco input hole, to pass through film
On hole, add water conservation biological organic fertilizer prepared by the common organic fertilizers of 60g or embodiment 1 to every plant of tobacco seedlings rhizosphere, then
Pour 300ml root waters.
Three processing of Setup Experiments, be respectively：
(1) compare, common organic fertilizer or water conservation biological organic fertilizer are not applied；
(2) common organic fertilizer, using common organic fertilizer；
(3) water retention organic fertilizer (prepared by embodiment 1), using water conservation biological organic fertilizer.
After tobacco seedlings are transplanted 30 days, determine cigarette strain underface 0~10cm soil moisture contents and determine the fresh weight of cigarette strain.
The fresh weight for determining 0~10cm soil moisture contents and tobacco plant is distinguished as depicted in figs. 1 and 2, using common organic
Fertilizer and biological water-keeping organic fertilizer can significantly increase the water content of 0~10cm soil and the fresh weight of tobacco plant.Compared to
Control treatment, organic fertilizer processing soil moisture content and plant fresh weight add 5.2% and 8.9%, water retention organic fertilizer processing respectively
Soil moisture content and plant fresh weight add 32.7% and 55.6% respectively.Water conservation biological organic fertilizer can significantly increase cigarette strain
The water content of rhizosphere soil and the fresh weight of seedling stage cigarette strain.
Leftover bits and pieces residue of monosodium glutamate and sugar industry industry of the present invention by using microbial strains fermentation glutamate production industry
Leftover bits and pieces molasses the like waste, either fermentation substrate, or tunning are all nontoxic, it is adaptable to which the cultivation of tobacco is used；And
Secondary fermentation substrate uses vinegar grain compost, and adding substantial amounts of rice husk due to the fermentation process of vinegar increases the aeration of fermentation substrate,
Rice husk content is higher in the composting material, therefore, and product of the invention can increase the hole of soil, compared to the ox become thoroughly decomposed
The compost such as excrement, chicken manure, are more beneficial for the effectively water conservation material such as γ-polyglutamic acid and moisture storage are held in into interstices of soil.
Claims (6)
1. one grows tobacco water conservation biological organic fertilizer, it is characterised in that the tobacco water conservation biological organic fertilizer is by with secretion γ-many
The bacillus amyloliquefaciens IAE of polyglutamic acid function is prepared through fermenting twice, wherein, the tobacco water conservation biological organic fertilizer
Contain 0.5 × 108cfu g-1Bacillus amyloliquefaciens IAE, γ above-polyglutamic acid content >=21g kg-1Dry weight, it is aqueous
Amount≤30%, the content of organic matter >=45%, wherein, the fermentation substrate of fermenting twice is respectively one grade fermemtation substrate and second order fermentation
Substrate, wherein, described one grade fermemtation substrate includes the component of following parts by weight：150~200 parts of residue of monosodium glutamate, bean cake powder 70
~90 parts, 20~30 parts of molasses slag and MgSO40.5 part is prepared from；Described second order fermentation substrate includes following parts by weight
Component：20~30 parts of 10~15 parts of humic acid and vinegar grain compost；Described vinegar grain compost is the vinegar grain compost after becoming thoroughly decomposed, germination
Index more than 98%, quality of organic matter are 30%~35% than content >=45%, water content mass ratio.
2. the tobacco water conservation biological organic fertilizer according to described in claim 1, it is characterised in that described one grade fermemtation substrate it is each
After component mixing, regulation water content is standby after 50~55%, 115 DEG C of sterilizing 30min.
3. the preparation method of the tobacco water conservation biological organic fertilizer described in claim 1, it is characterised in that comprise the following steps：
(1) bacillus amyloliquefaciens IAE is inoculated into liquid seed culture medium culture, condition of culture is：37 DEG C of cultivation temperature, shakes
Bed 130~160rpm of rotating speed, incubation time 24 hours, seed bacteria containing amount is more than 1 × 109cfu ml-1, then by seed according to
5% volume ratio is inoculated into progress liquid fermentation production in liquid fermentation medium, and the condition of fermenting and producing is：Cultivation temperature 28
~35 DEG C, dissolved oxygen throughput scope is 30~100%, 120~160rpm, wherein fermentation latter portions bacterial strain formation gemma, bacterium
CFU >=1 × 10 of strain9cfu ml-1, obtain bacillus amyloliquefaciens IAE zymotic fluid；
(2) by 150~200g of residue of monosodium glutamate, 70~90g of bean cake powder, molasses slag 20~30g and MgSO40.5g is mixed, and is adjusted aqueous
The 30min that sterilized at 50~55wt%, 115 DEG C is measured, one-level solid fermentation matrix is obtained, accumulated according to one-level solid fermentation matrix body
The zymotic fluid to bacillus amyloliquefaciens IAE that 5% ratio inoculation step (1) is obtained, 1~2 is piled by above-mentioned fermentation substrate
Start turning when meter Kuan, bar pile 0.8~1.2 meter high, 55~60 DEG C of bar pile central temperature, according to the difference of ambient temperature,
Fermentation 15~20 days, obtains one time fermentation product；
(3) the one time fermentation product obtained by step (2) and humic acid are mixed, standing stablizes 3~5h, stands the solid after stabilization
Thing is mixed with the vinegar grain compost after becoming thoroughly decomposed, and secondary fermentation 3~5 days obtains secondary fermentation product, wherein, one time fermentation product, corruption
Grow the ratio of weight and number 50~55: 10~15: 40~50 of acid and vinegar grain compost；
(4) by product low temperature drying after the secondary fermentation obtained by step (3) to water content mass ratio≤30%, packaging produces tobacco
Water conservation biological organic fertilizer.
4. preparation method according to claim 3, it is characterised in that in step (1), described liquid seed culture medium
It is formulated and is, according to 1L culture medium：Beef extract 3g, peptone 10g, NaCl 5g, running water 1000ml, pH scope 7.0~7.5,
It is standby after 121 DEG C of sterilizing 20min.
5. preparation method according to claim 3, it is characterised in that in step (1), liquid fermentation medium used is matched somebody with somebody
Fang Wei, is prepared according to 1L culture mediums：Glucose 80g, bean powder 30g, sodium glutamate 2g, (NH4)2SO46g, K2HPO42g, MgSO4
0.25g, MnSO40.2g, distilled water 1000ml, pH value are naturally, standby after 115 DEG C of sterilizing 30min.
6. preparation method according to claim 3, it is characterised in that the vinegar grain compost after being become thoroughly decomposed used by step (3) is hair
Bud index more than 98%, quality of organic matter are than content >=45%, the vinegar grain compost of water content mass ratio 30%~35%.
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|---|---|---|---|
| CN201510031541.5A CN104744182B (en) | 2015-01-21 | 2015-01-21 | One grows tobacco water conservation biological organic fertilizer and preparation method thereof |
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| CN105567243A (en) * | 2015-12-16 | 2016-05-11 | 罗永城 | Humic acid-type inorganic-organic water-retaining agent utilizing waste liquid of vinegar factory and preparation method for humic acid-type inorganic-organic water-retaining agent |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105295934A (en) * | 2015-10-26 | 2016-02-03 | 富川富兴果蔬有限责任公司 | Novel water-retaining agent using vinegar factory liquid waste and preparation method thereof |
| CN105542783A (en) * | 2015-12-16 | 2016-05-04 | 罗永城 | Humic acid type inorganic water-retaining agent by using edible vinegar factory waste liquid and preparation method thereof |
| CN105542778A (en) * | 2015-12-16 | 2016-05-04 | 罗永城 | Humic acid type organic water-retaining agent and preparation method thereof |
| CN105542780A (en) * | 2015-12-16 | 2016-05-04 | 罗永城 | Novel water-retaining agent by using edible vinegar factory waste liquid and preparation method thereof |
| CN105542777A (en) * | 2015-12-16 | 2016-05-04 | 罗永城 | Humic acid type water-retaining agent by using edible vinegar factory waste liquid and preparation method thereof |
| CN106518350A (en) * | 2016-10-27 | 2017-03-22 | 六安市振清生态农业有限公司 | Nutritive organic matrix for taxus chinensis planting |
| CN107058151B (en) * | 2016-11-29 | 2021-08-20 | 广东森度生态农业科技有限公司 | Microbial bacterium with fermentation water retention function and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1648107A (en) * | 2005-01-10 | 2005-08-03 | 左淑珍 | Biological organic fertilizer and its preparing method |
| CN101786916A (en) * | 2010-03-23 | 2010-07-28 | 山西晨雨科技开发连锁经营有限公司 | Bio-fertilizer produced by fermenting steel slag, vinegar residue and weathered coal through microbes and preparation method thereof |
| CN102863288A (en) * | 2012-09-28 | 2013-01-09 | 中国农业科学院烟草研究所青岛科技开发中心 | Organic-inorganic compound formula for tobacco |
| CN103172437A (en) * | 2012-12-31 | 2013-06-26 | 天津北洋百川生物技术有限公司 | Liquid compound microbial fertilizer containing polyglutamic acid, and preparation method and application thereof |
| CN103805204A (en) * | 2013-07-22 | 2014-05-21 | 河海大学 | Farmland soil microorganism water-holding product and preparation method thereof |
| CN103804067A (en) * | 2013-07-22 | 2014-05-21 | 河海大学 | Biological agent for supporting calcium to facility plantation crops as well as preparation method and application of biological agent |
-
2015
- 2015-01-21 CN CN201510031541.5A patent/CN104744182B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1648107A (en) * | 2005-01-10 | 2005-08-03 | 左淑珍 | Biological organic fertilizer and its preparing method |
| CN101786916A (en) * | 2010-03-23 | 2010-07-28 | 山西晨雨科技开发连锁经营有限公司 | Bio-fertilizer produced by fermenting steel slag, vinegar residue and weathered coal through microbes and preparation method thereof |
| CN102863288A (en) * | 2012-09-28 | 2013-01-09 | 中国农业科学院烟草研究所青岛科技开发中心 | Organic-inorganic compound formula for tobacco |
| CN103172437A (en) * | 2012-12-31 | 2013-06-26 | 天津北洋百川生物技术有限公司 | Liquid compound microbial fertilizer containing polyglutamic acid, and preparation method and application thereof |
| CN103805204A (en) * | 2013-07-22 | 2014-05-21 | 河海大学 | Farmland soil microorganism water-holding product and preparation method thereof |
| CN103804067A (en) * | 2013-07-22 | 2014-05-21 | 河海大学 | Biological agent for supporting calcium to facility plantation crops as well as preparation method and application of biological agent |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105567243A (en) * | 2015-12-16 | 2016-05-11 | 罗永城 | Humic acid-type inorganic-organic water-retaining agent utilizing waste liquid of vinegar factory and preparation method for humic acid-type inorganic-organic water-retaining agent |
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|---|---|
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