CN108728379A - A kind of composite bacteria agent and preparation method thereof for feces of livestock and poultry quick composting - Google Patents
A kind of composite bacteria agent and preparation method thereof for feces of livestock and poultry quick composting Download PDFInfo
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Abstract
The invention discloses a kind of composite bacteria agent for feces of livestock and poultry quick composting, the composite bacteria agent includes husky good fortune Bacillus strain and lichem bacillus strain；And disclose preparation method.The present invention has the advantages that：Two plants of bacterium in composite bacteria agent of the present invention can symbiosis well, without antagonism between bacterial strain；The preparation method of microbial inoculum is easy to operation, and cost of manufacture is cheap；Main application is in the quick composting of low temperature season livestock excrement composting, have the function of reinforcing the decomposition of organic matter in feces of livestock and poultry, reduce nitrogen loss rate, eliminate stink, shorten the decomposed time, improve decomposed product quality, every physical and chemical index of composting production is superior to not add the compost of microbial inoculum.Compared with the control, it is added to the feces of livestock and poultry of this microbial inoculum, heap temperature ramp-up rate is apparently higher than not plus the control group of any microbial inoculum, compost moisture content decrease speed are faster than control group, H2The burst size of S gases reduces, and nitrogen loss rate reduces, and the organic carbon of composting production improves.
Description
Technical field
The present invention relates to microorganism formulation technical fields, and in particular to a kind of compound bacteria for feces of livestock and poultry quick composting
Agent and preparation method thereof.
Background technology
With the continuous development of China's livestock and poultry cultivation industry, the animal waste quantity generated every year also increases year by year, such as
These offal treatments of fruit are improper, it will environment caused seriously to pollute, and then health to human body and livestock and poultry cultivation industry
Sustainable development generates serious threat.How to greatest extent innoxious, recycling treatment fowl and animal excrement, that is, reach improvement ring
Border, and the purpose promoted economic development, it is important by having to the sustainable development of the protection of china natural resources and environment and economy
Realistic meaning.High temperature aerobic composting is processing the most frequently used most viable method of fowl and animal excrement.Compost treatment can be to greatest extent
Mitigate pollution of the feces of livestock and poultry to environment, compost product can be used as the fertilizer needed for plant growth, promote plant growth, improves and makees
Produce amount.
Contain a large amount of microorganism in the material of aerobic compost, these microorganisms are by generating catabolic enzyme in compost material
Organic substance decomposed.External source microbial inoculum is added in compost material, and micro organism quantity and type in compost material is made to increase,
Accelerate the decomposition to organic substance, improves the quality of composting production.Liu Changli etc. is added in the research that external source microbial inoculum makes compost,
The compost of microbial inoculum is added compared with not adding microbial inoculum, adds the heap temperature of microbial inoculum up to 63.0 DEG C, 55.0 DEG C or more of megathermal period
It it is 7 days, the compost maturity time than being not added with microbial inoculum shortens 17 days.The tomato seeds and tomato seedling cultivated with composting production
Growing way, the observation group for adding microbial inoculum are substantially better than the control group for not adding microbial inoculum.Also scholar is studies have shown that the addition micro- life of external source
Object microbial inoculum can eliminate stool odor and reduce nitrogen loss rate, improve organic matter, total nitrogen, full phosphorus, full potassium in composting production and contain
Amount.
Cellulose is the chief component of cow dung and agricultural crop straw and other agriculture organic solid castoffs, is cow dung
Primary raw material in composting process, the content of cellulose are to be limited in the principal element of compost, but most of lignocellulosics are difficult
With degradation, the degradation of the decomposed and cellulose family of compost is closely related.Studies have shown that being inoculated with cellulose degradation in composting process
Bacterium, can promote cellulose degradation, accelerate organic substance and mineralize speed, reduce the carbon-nitrogen ratio in composting process, improve decomposed speed
Rate.
Invention content
The purpose of the present invention provides a kind of quickly rotten for feces of livestock and poultry aiming at above-mentioned defect in the prior art
Ripe composite bacteria agent and preparation method thereof.
To achieve the goals above, technical solution provided by the invention is：A kind of answering for feces of livestock and poultry quick composting
Combined bacteria agent, the composite bacteria agent include husky good fortune bacillus(Bacillus safensis)Bacterial strain and bacillus licheniformis（Bacillus licheniformis)Bacterial strain；
The sand good fortune Bacillus strain, deposit number is CGMCC NO.15809, is named as husky good fortune bacillus GWX1-2a,
The preservation time is on May 24th, 2018, and preservation mechanism is China General Microbiological culture presevation administrative center, and address is Beijing
The institute 3 of Chaoyang District North Star West Road 1, Institute of Microorganism, Academia Sinica；
The lichem bacillus strain, deposit number are CGMCC NO.15810, are named as bacillus licheniformis X10, preservation
Time is on May 24th, 2018, and preservation mechanism is China General Microbiological culture presevation administrative center, and address is Beijing's southern exposure
The institute 3 of area North Star West Road 1, Institute of Microorganism, Academia Sinica.
Further, above-mentioned a kind of composite bacteria agent for feces of livestock and poultry quick composting, the composite bacteria agent is by sand
Good fortune Bacillus strain and lichem bacillus strain are according to volume ratio 1:1 ratio is mixture prepared.
There is provided a kind of systems of above-mentioned composite bacteria agent for feces of livestock and poultry quick composting for second object of the present invention
Preparation Method includes the following steps：
1）The preparation of husky good fortune fermentation of bacillus liquid：
In fermentation tank, fermentation medium liquid amount is 55%~65%, and husky good fortune bacillus seed liquor access amount is 0.5%~2%,
33 DEG C~37 DEG C of fermentation temperature, mixing speed are 280~320r/min, and ventilatory capacity is 5.5~6.5 L/min, and fermentation termination is
12~33 hours, it is spare that husky good fortune fermentation of bacillus liquid was preserved after fermentation；
2）The preparation of the lichen bacillus ferments liquid：
In fermentation tank, fermentation medium liquid amount is 55%~65%, and bacillus licheniformis seed liquor access amount is 0.5%~2%,
33 DEG C~37 DEG C of fermentation temperature, mixing speed are 280~320r/min, and ventilatory capacity is 5.5~6.5 L/min, fermentation termination 6
~25 hours, it is spare that the lichen bacillus ferments liquid was preserved after fermentation；
3）The preparation of composite bacteria agent：
Step 1 is taken respectively）With step 2）Sand good fortune fermentation of bacillus liquid and the lichen bacillus ferments liquid each 100 obtained~
It 800 parts, is distributed into sterilized container and is sealed, finished product composite bacteria agent both can be obtained.
Further, the preparation method of above-mentioned a kind of composite bacteria agent for feces of livestock and poultry quick composting, the step
1）With step 2）In, the viable bacteria amount of husky good fortune fermentation of bacillus liquid and the lichen bacillus ferments liquid is more than 2 × 109cfu/mL。
Further, the preparation method of above-mentioned a kind of composite bacteria agent for feces of livestock and poultry quick composting, the step
1）In, the proportioning of fermentation medium is per addition peptone 5.0 g, NaCl 0.125g, K in 1000ml water2HPO40.5g,
MgSO40.2g, FeSO40.15g adjusts pH 7.2-7.4.
Further, the preparation method of above-mentioned a kind of composite bacteria agent for feces of livestock and poultry quick composting, the step
2）In, the proportioning of fermentation medium is that CMC-Na 5.0g, peptone 5.0 g, NaCl 0.125g is added in every 1000ml water,
K2HPO40.5g, MgSO40.2g adjusts pH 7.2-7.4.
Further, the preparation method of above-mentioned a kind of composite bacteria agent for feces of livestock and poultry quick composting, the step
3）In, husky good fortune fermentation of bacillus liquid and the lichen bacillus ferments liquid are according to volume ratio 1:What 1 ratio was uniformly mixed.
Strain in feces of livestock and poultry quick composting microbial bacterial agent of the present invention：Husky good fortune Bacillus and lichens gemma bar
Bacterium strain is the bacterial strain that Gansu Academy of Agricultural Science's soil and fertilizer independently detaches acquisition with water-saving research institute, through the Chinese Academy of Sciences
Institute of microbiology is accredited as husky good fortune bacillus and bacillus licheniformis.
A kind of feces of livestock and poultry quick composting microbial bacterial agent provided by the invention, mainly by bacillus licheniformis and Sha Fu
Bacillus carries out liquid fermentation respectively, and viable bacteria amount in zymotic fluid is made to be more than 2 × 109cfu/mL；Then in sterile environment,
Two kinds of zymotic fluids are pressed 1:1 ratio mixing, is finally dispensed into closed preservation in the molten device of sterilizing, is prepared into high-effective microorganism bacterium
Agent, the microbial inoculum are liquid bacterial agent, can directly be used.
Compared with prior art, the present invention has the advantages that：
1, the present invention is that 1 is pressed after lotion body ferment tank using husky good fortune bacillus and bacillus licheniformis as strain:1 is mixed
Conjunction is made, two plants of bacterium can symbiosis well, without antagonism between bacterial strain.
2, the preparation method of the efficient quick composting microbial inoculum of feces of livestock and poultry provided by the invention is easy to operation, cost of manufacture
It is cheap.
3, the efficient quick composting microbial inoculum of feces of livestock and poultry provided by the invention, is mainly used in low temperature season feces of livestock and poultry（Ox
Excrement）The quick composting of compost has the decomposition for reinforcing organic matter in feces of livestock and poultry, reduction nitrogen loss rate, elimination stink, shortening rotten
Ripe time, the effect for improving decomposed product quality, every physical and chemical index of composting production are superior to not add the compost of microbial inoculum.With
Control is compared, and the feces of livestock and poultry of this hair microbial bacterial agent is added to, and heap temperature ramp-up rate is apparently higher than pair for not adding any microbial inoculum
According to group, compost moisture content decrease speed is faster than control group, H2The burst size of S gases reduces 54.24%, and nitrogen loss rate reduces
58.77%, the organic carbon of composting production improves 8.61%.
Description of the drawings
Fig. 1 is shown as husky Promised Land microbial inoculum（Patent of invention）Be not added with the handling of microbial inoculum, commercially availableIt is microbial inoculum, commercially availableMicrobial inoculum, city
It sellsThe H of microbial inoculum cow dung compost2S burst sizes are compared.
Fig. 2 is shown as husky Promised Land microbial inoculum（Patent of invention）Be not added with the handling of microbial inoculum, commercially availableIt is microbial inoculum, commercially availableMicrobial inoculum, city
It sellsThe NH of microbial inoculum cow dung compost3Burst size is compared.
Fig. 3 is shown as husky Promised Land microbial inoculum（Patent of invention）Be not added with the handling of microbial inoculum, commercially availableIt is microbial inoculum, commercially availableMicrobial inoculum, city
It sellsThe germination index of microbial inoculum cow dung compost is compared.
Fig. 4 is shown as husky Promised Land microbial inoculum（Patent of invention）Be not added with the handling of microbial inoculum, commercially availableIt is microbial inoculum, commercially availableMicrobial inoculum, city
It sellsThe full nitrogen variation of microbial inoculum cow dung compost is compared.
Fig. 5 is shown as husky Promised Land microbial inoculum（Patent of invention）Be not added with the handling of microbial inoculum, commercially availableIt is microbial inoculum, commercially availableMicrobial inoculum, city
It sellsThe organic carbon variation of microbial inoculum cow dung compost is compared.
Fig. 6 is shown as husky Promised Land microbial inoculum（Patent of invention）Be not added with the handling of microbial inoculum, commercially availableIt is microbial inoculum, commercially availableMicrobial inoculum, city
It sellsThe colibacillus of excrement quantity of microbial inoculum cow dung compost is compared.
Fig. 7 is shown as husky Promised Land microbial inoculum（Patent of invention）Be not added with the handling of microbial inoculum, commercially availableIt is microbial inoculum, commercially availableMicrobial inoculum, city
It sellsThe pH value of microbial inoculum cow dung compost is compared.
Fig. 8 is shown as husky Promised Land microbial inoculum（Patent of invention）Be not added with the handling of microbial inoculum, commercially availableIt is microbial inoculum, commercially availableMicrobial inoculum, city
It sellsThe windrow water content of microbial inoculum cow dung compost is compared.
Fig. 9 is shown as husky Promised Land microbial inoculum（Patent of invention）Be not added with the handling of microbial inoculum, commercially availableIt is microbial inoculum, commercially availableMicrobial inoculum, city
It sellsThe compost temperature variation of microbial inoculum cow dung compost is compared.Wherein, gaza Promised Land microbial inoculum（Patent of invention）Afterwards, compost initial stage heap temperature
Rise soon, duration megathermal period is short.
Specific implementation mode
Embodiment 1：
A kind of formula of feces of livestock and poultry quick composting microbial bacterial agent is：500 parts of the lichen bacillus ferments liquid, husky good fortune gemma bar
500 parts of fermented liquid.
Preparation method is as follows：
（1）The preparation of husky good fortune fermentation of bacillus liquid：
It is cultivated using the fermentation medium of pH 7.2（Medium component is：Peptone 5.0 g, NaCl 0.125g,
K2HPO40.5g, MgSO40.2g, FeSO40.15g, water 1000mL）, seed liquor access amount is 1.5%, 37 DEG C of fermentation temperature,
Mixing speed is 300r/min, and best ventilatory capacity is 6.5L/min, is fermented 12 hours, and it is standby that sand good fortune fermentation of bacillus liquid is made
With；
（2）The preparation of the lichen bacillus ferments liquid：
It is cultivated using the fermentation medium of pH 7.0（Medium component is：CMC-Na 5.0g, peptone 5.0 g, NaCl
0.125g, K2HPO40.5g, MgSO40.2g, water 1000mL）, seed liquor access amount is 2%, 35 DEG C of fermentation temperature, mixing speed
For 320r/min, best ventilatory capacity is 6.5L/min, is fermented 10 hours, and it is spare to preserve zymotic fluid after fermentation.
（3）The preparation of microbial inoculum：
Take step（1）And step（2）Sand good fortune fermentation of bacillus liquid obtained and each 500 parts of the lichen bacillus ferments liquid, are mixed
Conjunction stirs evenly, and is distributed into sterilized container and is sealed, and may be either finished product microbial inoculum.
Embodiment 2：
A kind of formula of cow dung quick composting microbial bacterial agent is：800 parts of the lichen bacillus ferments liquid, husky good fortune bacillus hair
800 parts of zymotic fluid.
Preparation method is as follows：
（1）The preparation of husky good fortune fermentation of bacillus liquid：
It is cultivated using the fermentation medium of pH 7.4（Medium component is：Peptone 5.0 g, NaCl 0.125g,
K2HPO40.5g, MgSO40.2g, FeSO40.15g, water 1000mL）, seed liquor access amount is 1.5%, 35 DEG C of fermentation temperature,
Mixing speed is 280r/min, and best ventilatory capacity is 6L/min, is fermented 24 hours, and it is standby that sand good fortune fermentation of bacillus liquid is made
With；
（2）The preparation of the lichen bacillus ferments liquid：
It is cultivated using the fermentation medium of pH 7.2（Medium component is：CMC-Na 5.0g, peptone 5.0 g, NaCl
0.125g, K2HPO40.5g, MgSO40.2g, water 1000mL）, seed liquor access amount is 1%, 33 DEG C of fermentation temperature, mixing speed
For 320r/min when, best ventilatory capacity be 5.5L/min, ferment 15 hours, after fermentation preserve zymotic fluid it is spare.
（3）The preparation of microbial inoculum：
Take step（1）And step（2）Sand good fortune fermentation of bacillus liquid obtained and each 800 parts of the lichen bacillus ferments liquid, are mixed
Conjunction stirs evenly, and is distributed into sterilized container and is sealed, and may be either finished product microbial inoculum.
Embodiment 3：
A kind of formula of cow dung quick composting microbial bacterial agent is：800 parts of the lichen bacillus ferments liquid, husky good fortune bacillus hair
200 parts of zymotic fluid.
Preparation method is as follows：
（1）The preparation of husky good fortune fermentation of bacillus liquid：
It is cultivated using the fermentation medium of pH 7.3（Medium component is：Peptone 5.0 g, NaCl 0.125g,
K2HPO40.5g, MgSO40.2g, FeSO40.15g, water 1000mL）, seed liquor access amount is 2%, and 37 DEG C of fermentation temperature stirs
It is 300r/min to mix speed, and best ventilatory capacity is 6.5L/min, is fermented 30 hours, and it is spare that sand good fortune fermentation of bacillus liquid is made；
（2）The preparation of the lichen bacillus ferments liquid：
It is cultivated using the fermentation medium of pH 7.1（Medium component is：CMC-Na 5.0g, peptone 5.0 g, NaCl
0.125g, K2HPO40.5g, MgSO40.2g, water 1000mL）, seed liquor access amount is 0.5%, 37 DEG C of fermentation temperature, stirring speed
Degree is 300r/min, and best ventilatory capacity is 6.5L/min, is fermented 24 hours, and it is spare to preserve zymotic fluid after fermentation.
（3）The preparation of microbial inoculum：
Take step（1）And step（2）Sand good fortune fermentation of bacillus liquid obtained and each 200 parts of the lichen bacillus ferments liquid, are mixed
Conjunction stirs evenly, and is distributed into sterilized container and is sealed, and may be either finished product microbial inoculum.
Embodiment 4：
Microbial bacterial agent compost Contrast on effect, as shown in figs. 1 to 9：
The compost contrast test of this microbial bacterial agent mainly uses feces of livestock and poultry quick composting microbial bacteria produced by the present invention
Agent is added microbial inoculum by 1 ‰ envelope-bulk to weight ratio, and is uniformly mixed with cow dung, adjusts cow dung water content to 65% or so, builds up 1.5
Meter Kuan, 1 meter of height, 20 meters long of heap carry out compost fermentation.Main compost effect has：
（1）It is added to the cow dung of this hair microbial bacterial agent, compost temperature rose to 60.33 DEG C at the 8th day, and did not added any microbial inoculum
The 10th talent of control group cow dung rises to 61.67 DEG C, and the cow dung heap temperature ramp-up rate for adding microbial bacterial agent is apparently higher than not plus appoints
The control group cow dung of what microbial inoculum.
（2）It is added to the cow dung of microbial bacterial agent of the present invention, compost moisture content decrease speed is faster than control group.
（3）The cow dung of microbial bacterial agent of the present invention is added to compared with the control group cow dung for not adding any microbial inoculum, H2S gases
Burst size reduce 54.24%, effectively reduce cow dung compost stink.
（4）The cow dung of microbial bacterial agent of the present invention is added to not plus compared with the control group cow dung of any microbial inoculum, compost produces
The organic carbon of product improves 8.61%, and nitrogen loss rate reduces 58.77%.
Finally it should be noted that：The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
With technical scheme described in the above embodiments is modified or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in the present invention's
Within protection domain.
Claims (7)
1. a kind of composite bacteria agent for feces of livestock and poultry quick composting, which is characterized in that the composite bacteria agent includes Sha Fuya
Spore bacillus(Bacillus safensis)Bacterial strain and bacillus licheniformis（Bacillus licheniformis)Bacterial strain；
The sand good fortune Bacillus strain, deposit number is CGMCC NO.15809, is named as husky good fortune bacillus GWX1-2a,
The preservation time is on May 24th, 2018, and preservation mechanism is China General Microbiological culture presevation administrative center；
The lichem bacillus strain, deposit number are CGMCC NO.15810, are named as bacillus licheniformis X10, preservation
Time is on May 24th, 2018, and preservation mechanism is China General Microbiological culture presevation administrative center.
2. a kind of composite bacteria agent for feces of livestock and poultry quick composting according to claim 1, which is characterized in that described multiple
Combined bacteria agent is by husky good fortune Bacillus strain and lichem bacillus strain according to volume ratio 1:1 ratio is mixed with to obtain
's.
3. a kind of preparation method of composite bacteria agent for feces of livestock and poultry quick composting according to claim 1, feature
It is, includes the following steps：
1）The preparation of husky good fortune fermentation of bacillus liquid：
In fermentation tank, fermentation medium liquid amount is 55%~65%, and husky good fortune bacillus seed liquor access amount is 0.5%~2%,
33 DEG C~37 DEG C of fermentation temperature, mixing speed are 280~320r/min, and ventilatory capacity is 5.5~6.5 L/min, and fermentation termination is
12~33 hours, it is spare that husky good fortune fermentation of bacillus liquid was preserved after fermentation；
2）The preparation of the lichen bacillus ferments liquid：
In fermentation tank, fermentation medium liquid amount is 55%~65%, and bacillus licheniformis seed liquor access amount is 0.5%~2%,
33 DEG C~37 DEG C of fermentation temperature, mixing speed are 280~320r/min, and ventilatory capacity is 5.5~6.5 L/min, fermentation termination 6
~25 hours, it is spare that the lichen bacillus ferments liquid was preserved after fermentation；
3）The preparation of composite bacteria agent：
Step 1 is taken respectively）With step 2）Sand good fortune fermentation of bacillus liquid and the lichen bacillus ferments liquid each 100 obtained~
It 800 parts, is distributed into sterilized container and is sealed, finished product composite bacteria agent both can be obtained.
4. a kind of preparation method of composite bacteria agent for feces of livestock and poultry quick composting according to claim 3, feature
It is, the step 1）With step 2）In, the viable bacteria amount of husky good fortune fermentation of bacillus liquid and the lichen bacillus ferments liquid is more than 2
×109cfu/mL。
5. a kind of preparation method of composite bacteria agent for feces of livestock and poultry quick composting according to claim 3, feature
It is, the step 1）In, the proportioning of fermentation medium is that peptone 5.0 g, NaCl 0.125g is added in every 1000ml water,
K2HPO40.5g, MgSO40.2g, FeSO40.15g adjusts pH 7.2-7.4.
6. a kind of preparation method of composite bacteria agent for feces of livestock and poultry quick composting according to claim 3, feature
It is, the step 2）In, the proportioning of fermentation medium is that CMC-Na 5.0g, 5.0 g of peptone is added in every 1000ml water,
NaCl 0.125g, K2HPO40.5g, MgSO40.2g adjusts pH 7.2-7.4.
7. a kind of preparation method of composite bacteria agent for feces of livestock and poultry quick composting according to claim 3, feature
It is, the step 3）In, husky good fortune fermentation of bacillus liquid and the lichen bacillus ferments liquid are according to volume ratio 1:1 ratio
Uniformly mixed.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110029073A (en) * | 2019-03-13 | 2019-07-19 | 广东工业大学 | A kind of method of feces of livestock and poultry high-efficiency aerobic compost bacterium and its aerobic compost |
| CN110699299A (en) * | 2019-11-14 | 2020-01-17 | 乌拉特前旗荣生大地生物科技饲料有限责任公司 | Bacillus licheniformis X173 strain for producing urease inhibitor and application thereof |
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Cited By (5)
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| CN110029073A (en) * | 2019-03-13 | 2019-07-19 | 广东工业大学 | A kind of method of feces of livestock and poultry high-efficiency aerobic compost bacterium and its aerobic compost |
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| CN111606762A (en) * | 2020-05-14 | 2020-09-01 | 标优美生态工程股份有限公司 | Rapid composting microbial inoculum and method for producing organic fertilizer by using same |
| CN115287215A (en) * | 2022-05-30 | 2022-11-04 | 内蒙古农业大学 | Straw degrading bacterium HXB11 and application thereof |
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