CN110521735A - Dedicated root rot prevention and treatment microbial inoculum of a kind of highland barley and preparation method thereof - Google Patents
Dedicated root rot prevention and treatment microbial inoculum of a kind of highland barley and preparation method thereof Download PDFInfo
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- CN110521735A CN110521735A CN201910981189.XA CN201910981189A CN110521735A CN 110521735 A CN110521735 A CN 110521735A CN 201910981189 A CN201910981189 A CN 201910981189A CN 110521735 A CN110521735 A CN 110521735A
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- highland barley
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
Abstract
The present invention discloses dedicated root rot prevention and treatment microbial inoculum of a kind of highland barley and preparation method thereof.Microbial inoculum of the present invention, based on parts by weight, raw material components are mainly made of 5-10 parts of fluorescent pseudomonas Pseudomonas fluorescens Xp-6 bacterial strain (CCTCC M 2018754) fermentation liquid, 5-10 parts of bacillus cereus Bacillus cereus Xp-4 bacterial strain (CCTCC M 2018751) fermentation liquid, 5-10 parts of bacillus pumilus Bacillus pumilus Xp-5 bacterial strain (CCTCC M 2018752) fermentation liquid, 40-60 parts decomposed of feces of livestock and poultry, 15~20 parts of organobentonite, 5~10 parts of potassium fulvate, 5~10 parts of humic acid.Microbial inoculum of the present invention is environmentally protective, and active bacterial strain is low temperature resistant, adapts to the environment of highland barley growing area High aititude, low temperature, and good in the survival of highland barley rhizosphere, and the efficient antagonism various plants pathogen of energy plays the role of preventing and treating highland barley root rot.In addition, the active bacterial strain in the microbial inoculum also has the multiple efficacies such as Soluble phosphorus, fixed nitrogen, secretion plant hormone, the growth of highland barley can be promoted.
Description
Technical field
The invention belongs to field of agricultural microbial technology, and in particular to a kind of dedicated root rot prevention and treatment microbial inoculum of highland barley and its
Preparation method.
Background technique
Highland barley (Hordeum vulgare L.var.nudum Hook.f.) i.e. naked barley, is Cultivate berley (Hordeum
Vulgare L.) mutation.In China Qinghai-xizang Plateau Region, highland barley is most important cereal crops, is not only the Tibetan area people's
Staple food also has brilliant medical value and Dietotherapy health value.It is recorded according to " Jingzhubencao ", highland barley has cold-dispelling, heat-clearing
Dampness elimination, peaceful lung Dingchuan and other effects, can control the diseases such as deficiency of yang deficiency of the kidney, be traditional Tibetan medicine material.Modern study then finds highland barley rich in more
The mineral elements such as kind of amino acid, dietary fiber, vitamin, beta glucan and calcium, magnesium, phosphorus, zinc, manganese, selenium, reduce blood lipid,
Increasing WeiDongLi Capsule, prevention and treatment altitude sickness, diabetes etc. has unique health-care effect.Therefore, in recent years using highland barley as raw material
Health food type it is more and more, be also in the market to increase year by year to the demand of highland barley.However, root rot causes highland barley
Severe Reduction, highland barley infect root rot after, the fresh weight and dry weight of root and bud, every plant Number of kernels all decline seriously, thus
Its total output is caused to decline.It is China, Tibetan area, western part that highland barley growing area, which is distributed mainly on, and low temperature is generally presented in these local climates
The characteristics of high humidity, under this environmental condition, plant growth is slow, and the ability for resisting pathogen infection is weak, therefore will lead to disease
Harmful big generation.Investigation discovery, the generation of highland barley root rot was more and more common in recent years, caused by endanger also increasingly
Greatly.
Chemical pesticide mainly is used for the prevention and treatment of highland barley root rot at present, but can not be ignored the drawbacks of chemical pesticide,
Firstly, chemical pesticide residual can threaten food safety, people's health is influenced；Secondly, chemical pesticide can cause brokenly environmental ecology
It is bad；In addition, chemical pesticide can also cause soil degradation, soil quality can be made to decline.In addition highland barley growing area is mostly High aititude
Area, temperature is low, and the decomposition of pesticide is more slow, and ecological environment is also more fragile, and pesticide endangers very caused by highland barley growing area
Greatly.Therefore, it develops and is suitable for the pesticide substitute of highland barley growing area and just seems very urgent.In recent years, biological control agents
The appearance of agent provides new thinking.Biological control microbial inoculum using with biocontrol microorganisms as main active.Biocontrol microorganisms are a major class
Has effects that the growth of inhibition plant pathogenic microorganisms, to reach the general designation of the microorganism of controlling plant diseases.These active bacterias
Strain is typically derived from natural environment, therefore not will cause environmental problem.In addition to this, certain bacterial strains with biological and ecological methods to prevent plant disease, pests, and erosion are usual
The effect of also having both Soluble phosphorus, fixed nitrogen, secretion plant hormone, plays the role of promoting plant growth.In recent years, biocontrol agent produces
Industry is quickly grown, and has multiple product currently on the market, still, existing product effect in terms of highland barley root rot prevention and treatment extremely has
Limit, one is any microorganism, the adaptable environment of institute is all limited, and highland barley due to the limitation of microorganism adaptability
Growing area height above sea level is mostly at 2500 meters or more, and growth period average daily temperature is at 10 DEG C or less.Under such circumstances, most of external micro-
Biology is difficult normal growth, and therefore, it is difficult to play activity；The second is microorganism has certain selectivity, micro- life to plant
Object microbial inoculum will play effect, and the most important condition is their ability to colonize in plant surface, and the compatibility of external microorganism and highland barley is poor,
Effect is extremely limited.
It would therefore be highly desirable to which a kind of (have efficient antagonism phytopathy with the excellent biocontrol bacterial strain being isolated from highland barley rhizospheric environment
The ability of opportunistic pathogen) it is raw material, microbial inoculum is made by fermentation technique, there is preventive effect for highland barley root rot, reach reduction pesticide
The purpose used.
Summary of the invention
The main object of the present invention is directed to both at home and abroad in the prior art, still not specifically for the biology of highland barley root rot
Microbial inoculum, existing other biological microbial inoculum are difficult to adapt to the special environmental condition of highland barley growing area, lead to the prevention and treatment of highland barley root rot
It depends on chemical pesticide unduly, is not only difficult to effective prevention and control highland barley root rot, even more causes ecological environmental pollution problem and food
New microbial resources, energy saving and protection environment are explored, excavate and utilized to the statuses such as safety problem.
The present invention screens excellent biocontrol bacterial strain, microbial inoculum is prevented and treated with certain dedicated root rot of formula test highland barley, for blueness
Highland barley production provides novel " environmentally friendly " biocontrol agent, partially to substitute chemical pesticide.Reach not only effective prevention and control disease, but also
Reduce the purpose of chemical pesticide bring environmental hazard.
To achieve the goals above, the present invention provides a kind of dedicated root rot of highland barley to prevent and treat microbial inoculum, based on parts by weight,
Raw material components are mainly by 5-10 parts of fluorescent pseudomonas Pseudomonasfluorescens Xp-6 bacterial strain fermentation liquor, waxy bud
5-10 parts of spore bacillus Bacillus cereus Xp-4 bacterial strain fermentation liquor, bacillus pumilus Bacilluspumilus Xp-5 bacterium
Strain 5-10 parts of fermentation liquid, 40-60 parts decomposed of feces of livestock and poultry, 15~20 parts of organobentonite, 5~10 parts of potassium fulvate, humic
5~10 parts of compositions of acid.
Further, the fluorescent pseudomonas Pseudomonasfluorescens Xp-6 bacterial strain has efficiently short of money
The effect of the plant pathogenic fungi of the categories such as anti-Fusarium, Rhizoctonia, Bipolaris is now preserved in Chinese Typical Representative culture
Object collection, deposit number are CCTCC M 2018754；The bacillus cereus Bacillus cereus Xp-4 bacterial strain tool
Play the role of the plant pathogenic fungi of the categories such as efficient antagonism Fusarium, Rhizoctonia, Bipolaris, have concurrently Soluble phosphorus and
Fixed nitrogen function, furthermore the bacterial strain can generate endotoxin, there is certain killing effect to pathogenic nematode, now be preserved in Chinese Typical Representative training
Object collection is supported, deposit number is CCTCC M2018751；The bacillus pumilus Bacillus pumilus Xp-5 bacterial strain
Have the function of the plant pathogenic fungi of the categories such as efficient antagonism Fusarium, Rhizoctonia, Bipolaris, have concurrently fixed nitrogen and
Secrete plant hormone function.It is now preserved in China typical culture collection center, deposit number is CCTCC M 2018752.
Further, the feces of livestock and poultry is one of cow dung, sheep dung, horsehit, pig manure, chicken manure or combination, before use
Need to be sufficiently decomposed, it should keep moisture content between 40~65% in digest process, sealed fermenting keeps it sufficiently decomposed；After decomposed
It shines dry doubling to crush, it is spare to cross 100~200 meshes.
Further, the organobentonite, potassium fulvate and rare earth concave convex rod be both needed to use preceding crushing, cross 100~
200 meshes are spare.
The present invention also provides a kind of preparation methods of the dedicated root rot prevention and treatment microbial inoculum of highland barley, after including the following steps:
(1) preparation of root rot prevention and treatment microbial inoculum carrier:
1. adding water-water reactor to macerate feces of livestock and poultry, keeps water content between 40~65%, dried after sealed fermenting, to water content
It is spare lower than 15%；
2. the raw materials such as organic swelling, potassium fulvate, humic acid and the decomposed and feces of livestock and poultry that dries are removed miscellaneous powder respectively
After broken, 100~200 meshes are crossed；
3. each raw material is mixed in proportion；
4. 2h that the carrier of above-mentioned mixing is sterilized under 121 DEG C, 0.1MPa high-temperature and high-pressure conditions is prepared after being cooled to room temperature
It is spare for sterilized carrier；
(2) preparation of root rot prevention and treatment microbial inoculum:
1. prepare LB liquid medium, respectively be inoculated with fluorescent pseudomonas Xp-6 bacterial strain, bacillus cereus Xp-4 bacterial strain,
Bacillus pumilus Xp-5 bacterial strain, inoculum concentration are 5%, at 28~30 DEG C, 180r/min, cultivate 24~48 hours, use blood cell
Tally calculates thalline quantity in culture solution under the microscope, the concentration of thallus in culture solution is calculated with this, when in culture solution
Cell concentration reaches 109Culture is terminated when cfu/mL, and 3 kinds of bacterium solutions are mixed according to the ratio of volume ratio 1:1:1；
2. cultured mixed bacteria liquid and sterilized carrier are mixed in proportion with bacterium solution, and aseptically
Be placed in 28~30 DEG C of cultures 5-7 days it is spare, or be stored at room temperature in sterile sealed containers spare.
Further, the fluorescent pseudomonas Xp-6 bacterial strain, bacillus cereus Xp-4 bacterial strain, bacillus pumilus
Cell concentration of the Xp-5 bacterial strain in culture solution reaches 109Culture is terminated when cfu/mL or more.
Further, seed dressing uses when the microbial inoculum is suitable for plantation, usage amount and application method are as follows:
1. it is in advance that ploughing soil is soft, prepare sowing；
2. getting out highland barley seed, seed is placed on clean plastic cloth, it is anti-to add the dedicated root rot of the highland barley
Microbial inoculum is controlled, is stirred evenly, usage amount is that every double centner highland barley seed uses 5 kilograms of microbial inoculum；
3. preparing suitable cold water to be sprinkling upon on the seed of mixing, mix well, the microbial inoculum is all adhered on seed
Until；
4. it is loose to seed that the seed mixed is dried in the air on plastic cloth；
5. sowing according to a conventional method.
The fluorescent pseudomonas Xp-6 bacterial strain, bacillus cereus Xp-4 bacterial strain, bacillus pumilus Xp-5 bacterial strain, say
It is bright as follows:
Fluorescent pseudomonas Pseudomonas fluorescens Xp-6 bacterial strain have efficient antagonism Fusarium,
The effect of the plant pathogenic fungi of the categories such as Rhizoctonia, Bipolaris is preserved in Chinese Typical Representative on November 5th, 2018
Culture collection；Preservation address: Wuhan University；Deposit number is CCTCC M 2018754；Classification naming: Pseudomonas
fluorescens Xp-6
Bacillus cereus Bacillus cereus Xp-4 bacterial strain have efficient antagonism Fusarium, Rhizoctonia,
The effect of the plant pathogenic fungi of the categories such as Bipolaris has Soluble phosphorus and fixed nitrogen function concurrently.It is preserved in on November 5th, 2018
China typical culture collection center, preservation address: Wuhan University；Deposit number is CCTCC M 2018751；Classification naming:
Bacillus cereus Xp-4。
Bacillus pumilus Bacillus pumilus Xp-5 bacterial strain have efficient antagonism Fusarium,
The effect of the plant pathogenic fungi of the categories such as Rhizoctonia, Bipolaris has fixed nitrogen and secretion plant hormone function concurrently.In
On November 5th, 2018 is preserved in China typical culture collection center, preservation address: Wuhan University；Deposit number is CCTCC M
2018752；Classification naming: Bacillus pumilus Xp-5.
The dedicated root rot of a kind of highland barley of the present invention prevents and treats microbial inoculum and preparation method, the beneficial effect is that:
(1) the dedicated root rot prevention and treatment microbial inoculum of highland barley of the present invention has the function of efficient antagonism various plants pathogen,
And the microbial inoculum is environmentally protective, will not damage to soil micro-ecosystem, will not cause pesticide residue；
(2) dedicated root rot prevention and treatment its active bacterial strain of microbial inoculum of highland barley of the present invention can be good at adapting to highland barley growing area
The environment of low temperature, High aititude, and bacterial strain is affinity with highland barley, can be good at surviving in highland barley rhizosphere, therefore its effect
Well.
(3) microbial inoculum carrier raw material of the present invention has decomposed feces of livestock and poultry, organobentonite, potassium fulvate, humic acid
It forms, five poisonous creatures: scorpion, viper, centipede, house lizard, toad itself is harmless and full of nutrition in mentioned component, provides the nutrition of general equilibrium to highland barley, can substitute part
Fertilizer；
(4) microbial inoculum of the present invention has the function of improveing soil, can prevent soil degradation and soil hardening；
(5) microbial inoculum of the present invention is low in cost, and support material feces of livestock and poultry etc. is agriculture wastes, efficient to its
It utilizes, not only saves economic cost, increase economic efficiency, and can turn waste into wealth, realize recycling for resource.
Specific embodiment
Embodiment 1
Fluorescent pseudomonas Xp-6 bacterial strain, bacillus cereus Xp-4 bacterial strain, bacillus pumilus Xp-5 bacterial strain are mixed
Culture, the experimental results showed that hybrid bacterial strain well-grown at 10~15 DEG C.To the hybrid bacterial strain system of determination, two plants of bacterium
Between without Antagonistic reaction, composite bacteria agent enhances the antagonistic ability of pathogen, individually cultivates phase with each bacterial strain using after compound strain
Than the indexs such as Soluble phosphorus, fixed nitrogen also have apparent increase.
Composite bacteria agent and single strain microbial inoculum using effect experimental result are as shown in table 1, it was demonstrated that fluorescent pseudomonas Xp-6 bacterium
Not only make between each other without antagonism when strain, bacillus cereus Xp-4 bacterial strain, bacillus pumilus Xp-5 bacterial strain mixed culture
With, and the effect also mutually promoted, achieve the effect that 1+1 is greater than 2.
Table 1: composite bacteria agent and single strain microbial inoculum using effect compare
Embodiment 2
The dedicated root rot of a kind of highland barley of the present invention prevents and treats microbial inoculum, in parts by weight, based on parts by weight, component
Mainly by 40 parts decomposed of feces of livestock and poultry, 20 parts of organobentonite, 10 parts of potassium fulvate, 10 parts of humic acid, 20 parts of mixed bacteria liquid
Composition.The mixed bacteria liquid is by fluorescent pseudomonas Xp-6 bacterial strain fermentation liquor, bacillus cereus Xp-4 bacterial strain fermentation liquor, short and small
Bacillus Xp-5 bacterial strain fermentation liquor is formed in the ratio of 1:1:1.
The microbial inoculum the preparation method is as follows:
1. adding water-water reactor to macerate feces of livestock and poultry, keeps water content between 40~65%, water content is dried to after sealed fermenting
It is spare lower than 15%；
2. the raw materials such as organic swelling, potassium fulvate, humic acid and the decomposed and feces of livestock and poultry that dries are removed miscellaneous powder respectively
After broken, 100~200 meshes are crossed；
3. each raw material is mixed；
4. 2h that the carrier of above-mentioned mixing is sterilized under 121 DEG C, 0.1MPa high-temperature and high-pressure conditions is prepared after being cooled to room temperature
It is spare for sterilized carrier；
(2) preparation of root rot prevention and treatment microbial inoculum:
1. prepare LB liquid medium, respectively be inoculated with fluorescent pseudomonas Xp-6 bacterial strain, bacillus cereus Xp-4 bacterial strain,
Bacillus pumilus Xp-5 bacterial strain, inoculum concentration are 5%, at 28~30 DEG C, 180r/min, cultivate 24~48 hours, use blood cell
Tally calculates thalline quantity in culture solution under the microscope, the concentration of thallus in culture solution is calculated with this, when in culture solution
Cell concentration reaches 109Culture is terminated when cfu/mL, and 3 kinds of bacterium solutions are mixed according to the ratio of volume ratio 1:1:1；
2. cultured bacterium solution and sterilized carrier are uniformly mixed, and aseptically it is placed in 28~30 DEG C of cultures
5-7 days stand-by, or is stored at room temperature for use in sterile sealed containers.
Field experiment
(1) experimental material: experiment is the microbial inoculum prepared according to above-mentioned formula and method with microbial inoculum, is compareed not apply any bacterium
Agent or pesticide.Plant choosing highland barley is used in experiment, and kind is dark blue.Select full, pure seed spare.
(2) test place: sample plot point is located at the new cities and towns in Gansu Province, Lintan County, Tibetan Autonomous Prefecture of Gannan.
(3) experimental method:
1. it is in advance that ploughing soil is soft, prepare sowing；
2. getting out seed, seed is placed on clean plastic cloth, adds bacterial manure, stir evenly, every double centner
Additive amount is 5 kilograms；
3. preparing suitable cold water to be sprinkling upon on the seed of mixing, mix well, notices that cold water cannot be added once, side
Side is mixed to add, until bacterial manure is all adhered on seed, but cannot be overly wet；
4. the seed mixed is dried in the air for a moment on plastic cloth, dry in the air to seed can be made loose and make to be sticked to the surface of the seed
Bacterial manure fall off；
5. sowing according to a conventional method；
(4) measurement of index
The highland barley florescence counts root rot disease incidence, plant height；The indexs such as yield, the mass of 1000 kernel of highland barley are counted after Grain Ripening.
Table 2: the using effect of special bacteria agent
As seen from the above table, the dedicated root rot prevention and treatment microbial inoculum of above-mentioned highland barley is applied, highland barley root rot can be effectively reduced
Incidence, and the growth of highland barley can be promoted, improve the yield and quality of highland barley.
The dedicated root rot prevention and treatment microbial inoculum storage of above-mentioned highland barley at room temperature, detects its viable count after 6 months, up to 6.15 ×
108cfu/g.Meet the regulation of " People's Republic of China's agricultural industry criteria (NY/T 1113-2006) ".
Embodiment 3
The dedicated root rot of a kind of highland barley of the present invention prevents and treats microbial inoculum, in parts by weight, based on parts by weight, component
Mainly it is made of 55 parts decomposed of feces of livestock and poultry, 15 parts of organobentonite, 5 parts of potassium fulvate, 5 parts of humic acid, 20 parts of bacterium solution.Institute
Bacterium solution is stated by fluorescent pseudomonas Xp-6 bacterial strain fermentation liquor, bacillus cereus Xp-4 bacterial strain fermentation liquor, bacillus pumilus Xp-
5 bacterial strain fermentation liquors are the ratio composition of 1:1:1 by volume.
The microbial inoculum the preparation method is as follows:
2. adding water-water reactor to macerate feces of livestock and poultry, water content is kept to dry water content between 40~65%, after sealed fermenting low
It is spare in 15%；
2. the raw materials such as organic swelling, potassium fulvate, humic acid and the decomposed and feces of livestock and poultry that dries are removed miscellaneous powder respectively
After broken, 100~200 meshes are crossed；
3. each raw material is mixed in proportion；
4. 2h that the carrier of above-mentioned mixing is sterilized under 121 DEG C, 0.1MPa high-temperature and high-pressure conditions is prepared after being cooled to room temperature
It is spare for sterilized carrier；
(2) preparation of root rot prevention and treatment microbial inoculum:
1. prepare LB liquid medium, respectively be inoculated with fluorescent pseudomonas Xp-6 bacterial strain, bacillus cereus Xp-4 bacterial strain,
Bacillus pumilus Xp-5 bacterial strain, inoculum concentration are 5%, at 28~30 DEG C, 180r/min, cultivate 24~48 hours, use blood cell
Tally calculates thalline quantity in culture solution under the microscope, the concentration of thallus in culture solution is calculated with this, when in culture solution
Cell concentration reaches 109Culture is terminated when cfu/mL, and 3 kinds of bacterium solutions are mixed according to the ratio of volume ratio 1:1:1；
2. cultured bacterium solution and sterilized carrier are uniformly mixed, and aseptically it is placed in 28~30 DEG C of cultures
5-7 days stand-by, or is stored at room temperature for use in sterile sealed containers.
Field experiment
(1) experimental material: the experiment microbial inoculum that microbial inoculum is that above-mentioned formula and method are prepared, compare for do not apply any microbial inoculum or
Pesticide.Plant choosing highland barley is used in experiment, and kind is dark blue 2000.Select full, pure seed spare.
(2) test place: sample plot point is located at Gansu Province, Zhuoni County, the Tibetan Autonomous Prefecture of Gannan township Shen Zang.
(3) experimental method:
1. it is in advance that ploughing soil is soft, prepare sowing；
2. getting out seed, seed is placed on clean plastic cloth, adds bacterial manure, stir evenly, every double centner
Additive amount is 5 kilograms；
3. preparing suitable cold water to be sprinkling upon on the seed of mixing, mix well, notices that cold water cannot be added once, side
Side is mixed to add, until bacterial manure is all adhered on seed, but cannot be overly wet；
4. the seed mixed is dried in the air for a moment on plastic cloth, dry in the air to seed can be made loose and make to be sticked to the surface of the seed
Bacterial manure fall off；
5. sowing according to a conventional method；
(4) measurement of index
The highland barley florescence counts root rot disease incidence, plant height；The indexs such as yield, the mass of 1000 kernel of highland barley are counted after Grain Ripening.
Table 3: the using effect of special bacteria agent
As seen from the above table, the dedicated root rot prevention and treatment microbial inoculum of above-mentioned highland barley is applied, highland barley root rot can be effectively reduced
Incidence, and the growth of highland barley can be promoted, improve the yield and quality of highland barley.
The dedicated root rot prevention and treatment microbial inoculum storage of above-mentioned highland barley at room temperature, detects its viable count after 6 months, up to 6.15 ×
108cfu/g.Meet the regulation of " People's Republic of China's agricultural industry criteria (NY/T 1113-2006) ".
Claims (6)
1. a kind of dedicated root rot of highland barley prevents and treats microbial inoculum, it is characterised in that: based on parts by weight, raw material components are mainly by fluorescence vacation
5-10 parts of sporangium Pseudomonas fluorescens Xp-6 bacterial strain fermentation liquor, bacillus cereus Bacillus
5-10 parts of cereus Xp-4 bacterial strain fermentation liquor, Brevibacillus brevis Bacillus pumilus Xp-5 bacterial strain fermentation liquor 5-10
Part, 5~10 parts of 40-60 parts decomposed of feces of livestock and poultry, 15~20 parts of organobentonite, 5~10 parts of potassium fulvate, humic acid groups
At.
2. a kind of dedicated root rot of highland barley prevents and treats microbial inoculum as described in claim 1, it is characterised in that: the feces of livestock and poultry is ox
At least one of excrement, sheep dung, horsehit, pig manure, chicken manure, need decomposed before use, moisture content should be kept 40 in digest process
Between~65%, sealed fermenting keeps it sufficiently decomposed；Dry doubling is shone after decomposed to crush, and it is spare to cross 100~200 meshes.
3. a kind of dedicated root rot of highland barley prevents and treats microbial inoculum as described in claim 1, it is characterised in that: the organobentonite and Huang
Rotten acid potassium is both needed to using preceding crushing, and it is spare to cross 100~200 meshes.
4. the dedicated root rot of highland barley as described in any in claim 1-3 prevents and treats microbial inoculum, it is characterised in that: preparation method
Are as follows:
(1) preparation of root rot prevention and treatment microbial inoculum carrier:
1. adding water-water reactor to macerate feces of livestock and poultry, keeps water content between 40~65%, dry after sealed fermenting, be lower than to water content
15%, it is spare；
2. the raw materials such as organic swelling, potassium fulvate, humic acid and the decomposed and feces of livestock and poultry that dries are cleaned respectively after crushing,
Cross 100~200 meshes；
3. each raw material is proportionally mixed；
4. 2h that the carrier of above-mentioned mixing is sterilized under 121 DEG C, 0.1MPa high-temperature and high-pressure conditions is prepared as after being cooled to room temperature
The carrier of sterilizing, it is spare；
(2) preparation of root rot prevention and treatment microbial inoculum:
1. prepare LB liquid medium, respectively be inoculated with fluorescent pseudomonas Pseudomonas fluorescens Xp-6 bacterial strain,
Bacillus cereus Bacillus cereus Xp-4 bacterial strain, bacillus pumilus Bacillus pumilus Xp-5 bacterial strain, connect
Kind amount is 5%, at 28~30 DEG C, 180r/min, cultivates 24~48 hours, calculates culture under the microscope with blood counting chamber
Thalline quantity in liquid calculates the concentration of thallus in culture solution with this, when the cell concentration in culture solution reaches 109It is whole when cfu/mL
It only cultivates, 3 kinds of bacterium solutions is mixed according to the ratio of volume ratio 1:1:1；
2. cultured bacterium solution and sterilized carrier are mixed in proportion with bacterium solution, and aseptically it is placed in 28~
30 DEG C culture 5-7 days it is spare, or be stored at room temperature in sterile sealed containers spare.
5. a kind of preparation method of the dedicated root rot prevention and treatment microbial inoculum of highland barley as claimed in claim 4, it is characterised in that: described glimmering
Light pseudomonas Xp-6 bacterial strain, the thallus of bacillus cereus Xp-4 bacterial strain, bacillus pumilus Xp-5 bacterial strain in culture solution
Concentration reaches 109Culture is terminated when cfu/mL or more.
6. any dedicated root rot of the highland barley prevents and treats microbial inoculum or method for claim 4 preparation in claim 1-3
Highland barley dedicated root rot prevention and treatment microbial inoculum method of administration, it is characterised in that: seed dressing uses when the microbial inoculum is suitable for plantation, uses
Amount and application method are as follows:
1. it is in advance that ploughing soil is soft, prepare sowing；
2. getting out highland barley seed, seed is placed on clean plastic cloth, adds the dedicated root rot prevention and treatment bacterium of the highland barley
Agent stirs evenly, and usage amount is that every double centner highland barley seed uses 5 kilograms of microbial inoculum；
3. preparing suitable cold water to be sprinkling upon on the seed of mixing, mix well, until the microbial inoculum is all adhered on seed；
4. it is loose to seed that the seed mixed is dried in the air on plastic cloth；
5. sowing according to a conventional method.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111418611A (en) * | 2020-04-01 | 2020-07-17 | 甘肃省农业科学院植物保护研究所 | Growth-promoting and disease-preventing microbial agent special for barley crops and preparation method and application thereof |
| CN113667617A (en) * | 2021-08-06 | 2021-11-19 | 甘肃农业大学 | Special microbial agent for natural high-cold grassland and preparation method and application thereof |
| CN113880670A (en) * | 2021-09-03 | 2022-01-04 | 西藏珠峰华绿生态农业科技有限公司 | Organic fertilizer composition special for highland barley, organic fertilizer special for highland barley and application method thereof |
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| CN113880670A (en) * | 2021-09-03 | 2022-01-04 | 西藏珠峰华绿生态农业科技有限公司 | Organic fertilizer composition special for highland barley, organic fertilizer special for highland barley and application method thereof |
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