CN1150133A - Efficient biological compound fertilizer and its preparing process - Google Patents
Efficient biological compound fertilizer and its preparing process Download PDFInfo
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- CN1150133A CN1150133A CN 96109854 CN96109854A CN1150133A CN 1150133 A CN1150133 A CN 1150133A CN 96109854 CN96109854 CN 96109854 CN 96109854 A CN96109854 A CN 96109854A CN 1150133 A CN1150133 A CN 1150133A
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Abstract
An efficient biological compound fertilizer is made up through fermentations of several kinds of multifunctional bacillus cereus with P decomposing bacteria and rhizosphere azotobacteria respectively, and mixing different kinds of fermented liquids, and mixing with multiple vegetable trace elements and adsorbent, and features high number of active living bacteria, low cost and no environmental pollution. The fertilizer serves to provide nutrients, promote healthy growth of crops and formation of chlorophyll, make crops ripen earlier by 5-7 days, and increase yield by 12.16-32.56%.
Description
The present invention relates to efficient biological compound fertilizer and preparation method thereof, belong to the microorganism fertilizer field.
Using in a large number of chemical fertilizer and agricultural chemicals plays a part usefully undoubtedly to the development of agriculture production, but also brought soil compaction, and soil fertility descends, and the soil micro-ecosystem environment is damaged, the vicious cycle that crop is more and more serious to the dependency of chemical fertilizer.For this reason, the development microorganism fertilizer then is to alleviate soil compaction, recovers microecological balance, increases crop yield, the epoch trend of development environment protection ecologic agricultural.
In existing many kinds of microbial fertilizers,,, and make the fertilizer efficiency instability perhaps owing to the bacterial classification performance issue perhaps because bacterial classification is single.The purpose of this invention is to provide a kind of multi-functional, form more, use composite bio-fertilizer easily, can promote plant growth, promote chlorophyll to form, strengthen the crop photosynthesis effect, improve the crop resistance against disadvantage, and have remarkable and stable effect of increasing production.
Solution of the present invention is, there is the multiple genus bacillus of reciprocal symbiosis relation to make up from root or the leaf part of crop from filtering out with crop, and plant nutrient substances such as different types of plant hormone that their energy biosynthesizing are a large amount of and phytocytomine, they play regulates crop metabolism, promote plant growth, promote chlorophyll to form, strengthen the crop photosynthesis effect, and can make the crop premature ripening; These useful microorganisms also play the invasion and attack effect of the harmful germ of defence to crop, thereby improve the disease resistance ability of crop; Select the rhizosphere combination azotobacter of high nitrogenase activity simultaneously for use and can decompose the phosphate solubilizing bacteria of organophosphorus and inorganic phosphorus in the soil, and be furnished with the required trace element of plant growth.
Efficient biological compound fertilizer of the present invention comprises at least:
1. the mixed solution of microbial fermentation solution, wherein comprise at least: the mixture of the fermented liquid that genus bacillus and phosphate solubilizing bacteria, rhizosphere combination azotobacter are cultivated respectively, wherein said genus bacillus comprises: bacillus cereus (Bacilluscereus), bacillus brevis (Bacillus Brevis) and bacillus pumilus (Bacillus pumillus); Phosphate solubilizing bacteria comprises: huge genus bacillus of phosphorus decomposing (Bacillus megatherium phosphaticum) and pseudomonas fluorescens (Pseudomonas fluorescens); But also each only select for use in the described bacterium a kind of, two or more are used in combination.Selected bacillus cereus, bacillus brevis and bacillus pumilus, it is the genus bacillus of a large amount of plant hormones of energy biosynthesizing, Plant hormones regulators,gibberellins and various plants phytokinin, huge genus bacillus of phosphorus decomposing and pseudomonas fluorescens have the effect of decomposing organophosphorus and inorganic phosphorus in the soil.Wherein said rhizosphere combination azotobacter is klepsiella pneumoniae (Klebsiella pne umoniae) and enterobacter cloacae (Enterobacter cloacea), but also each only select for use wherein a kind of, two kinds be used in combination.The volume ratio of fermentation of bacillus liquid and phosphate solubilizing bacteria, rhizosphere association nitrogen fixation fermented liquid is followed successively by (2-3): (1-2): (3-5).The fermention medium that is used for genus bacillus and phosphate solubilizing bacteria is to contain soyflour or soybean cake powder at least, feed yeast powder or yeast decoction, dipotassium hydrogen phosphate, the aqueous solution of sodium-chlor; The fermention medium of rhizosphere combination azotobacter is the aqueous solution that contains sucrose, soyflour, potassium primary phosphate, sodium-chlor, sal epsom, Sodium orthomolybdate, boric acid and ferrous sulfate at least, and PH6.4-7.2, leavening temperature are 28-32 ℃.
2. the minimum plant element comprises at least: the compound of boron, zinc, copper, titanium, manganese, molybdenum, iodine, chlorine, potassium, silicon, iron, magnesium and sulphur, can select sodium tetraborate 1-5% for use, zinc sulfate 1-4%, copper sulfate 0.01-0.06%, titanium dioxide 0.1-0.5%, potassiumiodide 0.05-0.5%, Repone K 1-5%, water glass 0.4-0.8%, sal epsom 0.005-0.02%, ferrous sulfate 0.02-0.08%, Sodium orthomolybdate 0.01-0.05%.
3. sorbent material, carrier as absorption mixed fermentation liquid, availablely comprise at least: the humic sour soil, turfy soil, sepiolite powder, vermiculite power or brown coal powder can only select for use wherein a kind of, two kinds or more of mixing to use, the granularity of described various materials is the 80-150 order, and uses behind 90-120 ℃ of drying and sterilizing.
The weight percent of material among the above-mentioned 1-3 in described efficient biological compound fertilizer is followed successively by mixed fermentation liquid: the minimum plant element: the % of sorbent material=(26-38): (4-10) %: (60-70) %, making product through mixing, also can be that minimum plant element and the sorbent material that has adsorbed mixed bacteria liquid are packaged into product respectively in proportion.
In the efficient biological compound fertilizer of the present invention, total living bacteria count is (8-25) * 10
8Individual/gram, use Shi Jike seed dressing or seed dressing and add and spray, can add the water pouring again or mix and make base manure or topdress and use with chemical fertilizer.Efficient biological compound fertilizer of the present invention can be used for plants such as food crop, cash crop, vegetables, fruits, flowers at least.
The preparation method of efficient biological compound fertilizer of the present invention comprises at least:
One. microbial fermentation is cultivated
(1) triangular flask is cultivated
The skill ordinary method is prepared the nutrition bouillon media and is prepared each 2.5-4 liter of vinelandii substratum by following prescription (I), transfer PH6.8-7.2, be sub-packed in 500 milliliters the triangular flask the above-mentioned substratum of the bottled 120-150 milliliter of each triangle, 0.1MPa sterilization 30 minutes.
With aforesaid various genus bacillus, phosphate solubilizing bacteria, rhizosphere combination azotobacter, under aseptic condition, be inoculated in respectively in the triangular flask that nutrition bouillon media and vinelandii substratum are housed, placed on the shaking table shaking culture 2-3 days, culture temperature is 28-32 ℃.
(2) seeding tank fermentation culture
1. fermention medium preparation
In A, B, C, D, E seed fermentation jar, prepare a certain amount of substratum by following prescription (first), be used for the fermentation of genus bacillus and phosphate solubilizing bacteria, in F, G seed fermentation jar, prepare a certain amount of substratum by following prescription (second), be used for the fermentation of rhizosphere combination azotobacter.
Prescription (first) (by weight percentage): soyflour or soybean-cake flour 0.7-4%, Semen Maydis powder 0.5-2.5%, feed yeast powder 0.25-2.0% or yeast decoction 0.025-0.2%, dipotassium hydrogen phosphate 0.1-0.4%, sodium-chlor 0.25-0.6%, manganous sulfate 0.001-0.006%, other are water, transfer PH6.8-7.2,0.1MPa sterilization 30 minutes.
Prescription (second) (by weight percentage): sucrose 0.5-2.0%, feed yeast powder 0.5-2.0% or yeast decoction 0.05-0.2%, Sunmorl N 60S 0.25-1.5%, potassium primary phosphate 0.05-0.15%, sodium-chlor 0.02-0.08%, lime carbonate 0.2-0.4%, ferrous sulfate 0.001-0.003%, Sodium Glutamate 0.05-0.3%, Sodium orthomolybdate 0.001-0.003% boric acid 0.001-0.004%, manganous sulfate 0.001-0.003%, other are water, PH6.8-7.2,0.1MPa sterilization 30 minutes;
2. fermentation culture
When the substratum temperature in the seeding tank drops to 28-35 ℃, respectively with the genus bacillus not of the same race in the triangular flask, be seeded in A, B, the C seeding tank, phosphate solubilizing bacteria is seeded in D, the E fermentor tank, and the rhizosphere combination azotobacter is seeded in F, the G seeding tank, and inoculum size is (0.5-1.5) * 10
8Individual/the milliliter fermented liquid, control pH6.4-7.2, temperature 28-32 ℃, air flow 1: 0.25-1.0 treats that the bacterium number is (4-10) * 10 in A, B, C, D, the E fermentor tank
9Individual/milliliter, the bacterium number is (4-8) * 10 in F, the G fermentor tank
9Individual/when milliliter, stop fermentation.
(3) produce a jar fermentation
Except that the volumetric ratio seeding tank of producing jar enlarged 10 times, culture medium preparation, sterilization were fully identical with the preparation of above-mentioned seed tank culture base, but 10 times of culture medium preparation amount expansions.When the fermented liquid temperature in each production jar drops to 28-32 ℃, the fermented liquid in the seeding tank one by one correspondence be transferred to during A, B, C, D, E, F, G produce jar, control PH6.4-7.2, temperature 28-32 ℃, air flow 1: 0.25-1.0 treats that the bacterium number that A, B, C, D, E produce in the jar is (6-12) * 10
9Individual/milliliter, the bacterium number that F, G produce in the jar is (4-10) * 10
9During/milliliter, stop fermentation.
Two, be mixed and made into product
A, B, C, D, E, F, G are produced the fermented liquid in the jar, A: B: C: D: E: F: G=1 by volume successively: 1: 1: 1: put into dosing vessel be mixed into mixed fermentation liquid at 1: 2: 2, mixed fermentation liquid by weight 26-38 part, 60-70 part is the aforementioned adsorbent powder of 80-150 purpose in 90-120 ℃ of drying and sterilizing, granularity, the aforesaid minimum plant element of 4-10 part, be uniformly mixed into product, pack.According to the efficient biological compound fertilizer that the present invention makes, every gram contains aforesaid genus bacillus living bacteria count and is (6-18) * 10
8Individual, the living bacteria count of rhizosphere combination azotobacter (2.5-8.0) * 10
8Individual, total living bacteria count is (8-25) * 10
8Individual.
Efficient biological compound fertilizer of the present invention outstanding advantage compared with the prior art is, adopt multi-functional excellent species combination, effective minimum plant element formula and the culture medium prescription that can produce high living bacteria count product, efficient biological compound fertilizer of the present invention, use proof through test for many years: this composite fertilizer has the metabolism of the crop of adjusting, promote chlorophyll to form, strengthen photosynthesis, premature ripening 5-7 days, the harmful germ of defence is to the invasion and attack effect of crop, promote the healthy and strong growth of crop, make various crop have remarkable and stable effect of increasing production, and bring good economic benefit.
In order to be illustrated more clearly in the present invention, enumerate following examples, but it there is not any restriction to the present invention.
Embodiment 1
Its composition of a kind of efficient biological compound fertilizer of the present invention comprises: bacillus cereus, bacillus brevis, bacillus pumilus, the huge genus bacillus of phosphorus decomposing, klepsiella pneumoniae, 1: 1: 1 of enterobacter cloacae: 1: 1: 2: 290 kilograms of 2 fermented liquid mixed solutions, with be 620 kilograms of 80-150 purpose humic sour soils through 90-120 ℃ of drying and sterilizing granularity, 31.5 kilograms in zinc sulfate, 28.5 kilograms in Repone K, 18.5 kilograms of sodium tetraborates, 4.5 kilograms of titanium dioxide, 0.25 kilogram in copper sulfate, 0.67 kilogram in ferrous sulfate, 0.78 kilogram in sal epsom, 1.5 kilograms of potassiumiodides, 5.5 kilograms of water glass, after 0.125 kilogram of Sodium orthomolybdate mixes, be divided in the plastics bag sealing and preserve.
Above-mentioned rhizosphere combination azotobacter also can be klepsiella pneumoniae or enterobacter cloacae just, but the ratio of its fermented liquid and the fermented liquid of bacillus cereus, the huge genus bacillus of phosphorus decomposing, pseudomonas fluorescens, bacillus brevis, bacillus pumilus is 4: 1: 1: 1: 1: 1.
Embodiment 2
The preparation method of efficient biological compound fertilizer in the last example:
1, triangular flask is cultivated: according to aforesaid triangular flask seed culture medium compound method, preparation is used for the substratum of genus bacillus, phosphate solubilizing bacteria and rhizosphere combination azotobacter respectively, 0.1MPa sterilization 30 minutes.When being cooled to 28-32 ℃, bacillus cereus, bacillus brevis, bacillus pumilus, the huge genus bacillus of phosphorus decomposing, pseudomonas fluorescens are seeded in the triangular flask that the nutrition bouillon media is housed, klepsiella pneumoniae and enterobacter cloacae are seeded in the triangular flask that the fixed nitrogen substratum is housed, tampon beyond the Great Wall was in 28-32 ℃ of shaking table shaking culture 2-3 days.
2, seeding tank fermentation culture
In A, the B of 100 liters of capacity, C, D, E seeding tank, each prepares 80 liters of substratum, wherein contains soyflour 2.5%, Semen Maydis powder 0.75%, feed yeast powder 0.75%, potassium primary phosphate 0.02%, sodium-chlor 0.5%, manganous sulfate 0.02%, pH6.8-7.2.In 200 liters F, G seeding tank, each prepares 160 liters of rhizosphere association nitrogen fixation bacterium culture mediums, wherein contains sucrose 1%, soyflour 1.5%, feed yeast powder 0.75%, Sunmorl N 60S 0.5%, potassium primary phosphate 0.08%, sal epsom 0.05%, sodium-chlor 0.025%, ferrous sulfate 0.002%, Sodium orthomolybdate 0.001%, boric acid 0.001%, manganous sulfate 0.001%, pH6.8-7.2,0.1MPa sterilization 30 minutes.In 28-32 ℃, during the huge genus bacillus of bacillus cereus, bacillus brevis, bacillus pumilus, phosphorus decomposing, the pseudomonas fluorescens of cultivating in the triangular flask connect in A, B, C, D, E seeding tank, klepsiella pneumoniae and enterobacter cloacae are inoculated in F, the G seeding tank, and inoculum size is (0.75-2) * 10
8Individual/the milliliter fermented liquid, control pH6.4-7.2 keeps 28-32 ℃ of temperature, air flow 1: 0.25-1.0, and fermentation culture bacterium number to A, B, C, D, the E seeding tank is (6-10) * 10
9Individual/milliliter, the bacterium number is (4-8) * 10 in F, the G seeding tank
9Individual/milliliter, stop fermentation.
3, produce a jar fermentation
Except that the volume of producing jar with cultivate base unit weight enlarges 10 times than respective seed jar, used culture medium prescription, processing condition such as control of pH temperature and air flow are fermented consistent with seeding tank fully.The fermented liquid in the seeding tank one by one correspondence be transferred to A, B, C, D, E, F, G and produce and jar ferment, treat that A, B, C, D, the E bacterium number in producing jar is (6-12) * 10
9Individual/milliliter, the bacterium number that F, G produce in the jar is (4-10) * 10
9During/milliliter, stop fermentation.
Two, be mixed and made into product
1, from A, B, C, D, E, F, G produce jar, A: B: C: D: E: F: G=1 by volume successively: 1: 1: 1: emit fermented liquid in dosing vessel in be mixed into mixed fermentation liquid at 1: 2: 2.
2, percentage mixed 1 mixed fermentation liquid and minimum plant element and sorbent material by 29.0: 8.97: 64.03 by weight, and sealed packet is dressed up product.Sorbent material wherein is the mixture of the humic sour soil of 40% sepiolite powder and 60%, and in 90-120 ℃ of sterilization, granularity is 100 orders.Described genus bacillus also can be only be used in combination with wherein one or both of bacillus cereus, bacillus brevis, bacillus pumilus, phosphate solubilizing bacteria also can only be selected a kind of in huge genus bacillus of phosphorus decomposing and the pseudomonas fluorescens for use, but genus bacillus, phosphate solubilizing bacteria are 3: 2: 4 with the ratio of rhizosphere association nitrogen fixation fermented liquid.
Example is used in the test of efficient biological compound fertilizer of the present invention:
Example one winter wheat, test unit: arable farming institute of the Chinese Academy of Agricultural Sciences; Experimental cultivar CA8686, test is provided with 4 processing, 4 repetitions.Handle A, seed dressing (consumption 500g/ mu); Treatments B, seed dressing is in conjunction with spraying 2% solution in jointing stage, boot stage; Handle C, seed dressing sprays 2% solution in conjunction with jointing stage, filling stage; Handle D, contrast, spray clear water.Result such as table 1.
Table 1 winter wheat is executed the efficient biological compound fertilizer effect of increasing production
| Handle | Per mu yield (kg) | Average yield per mu (kg) | Volume increase | ||||
| ?I | ?II | ?III | ?IV | Kg/ mu | ?+％ | ||
| ??A ??B ??C ??D(ck) | ?510.1 ?509.3 ?510.9 ?400.2 | ?507.2 ?511.4 ?527.0 ?404.0 | ?495.3 ?517.0 ?515.2 ?406.8 | ?494.6 ?505.5 ?516.1 ?419.0 | ??501.8 ??510.8 ??517.3 ??407.5 | ?94.3** ?103.3** ?109.8** | ?23.14 ?25.34 ?26.94 |
Variance analysis: F between processing
α=154.22＞F
0.01=5.41, reach utmost point conspicuous level.
Example two paddy rice, test unit: clay fertilizer station, Tongshan County, Hubei; Experimental cultivar " prestige excellent 64 ", test is provided with 3 processing, 3 repetitions.Handle A, contrast; Treatments B contrasts and sprays 300g/ mu ten tillering phase 3 times; Handle C, contrast and spray the peat composed of rotten mosses ten tillering phase 3 times, 300g/ mu.Result such as table 2.
Table 2 paddy rice is executed the efficient biological compound fertilizer effect of increasing production
| Handle | District's group output (kg) | Average yield per mu (kg) | Volume increase | |||
| ????I | ????II | ????III | Kg/ mu | ??+％ | ||
| ?A(ck) ?B ?C | ????29.9 ????43.9 ????32.9 | ????30.0 ????37.5 ????33.5 | ????31.2 ????35.6 ????30.7 | ??304 ??390 ??324 | ??86* ??20 | ??28.3 ??6.6 |
Variance analysis: F between processing
α=8.5＞F
0.05=6.90, reach significance level.
Example three Chinese cabbages, test unit: Changping, Beijing institute of agricultural sciences; Experimental cultivar " new No. one of capital ", test is provided with 3 processing, 3 repetitions.Handle A, every mu of this composite fertilizer of usefulness 500g sprays 3 times; Treatments B, every mu with 12 milliliters of foliar spraies of growth promotor for crop 3 times; Handle C, contrast (ck), spray clear water.Result such as table 3.
Table 3 Chinese cabbage is executed the efficient biological compound fertilizer effect of increasing production
Variance analysis: F between processing
α=39.45＞F
0.01=9.78, reach utmost point conspicuous level.
| Handle | District's group output (kg) | Average yield per mu (kg) | Volume increase | |||
| ????I | ??II | ??III | Kg/ mu | ??+％ | ||
| ?A ?B ?C(ck) | ??162.4 ??139.0 ??127.0 | ??168.9 ??143.9 ??132.9 | ??164.0 ??149.0 ??124.3 | ??8255 ??7215 ??6405 | ??1850** ??810* | ??28.88 ??12.64 |
Claims (4)
1. efficient biological compound fertilizer is characterized in that described efficient biological compound fertilizer comprises at least:
1) mixed solution of microbial fermentation solution wherein comprises at least: the fermented liquid that genus bacillus and phosphate solubilizing bacteria, rhizosphere combination azotobacter ferment respectively, and available genus bacillus comprises: bacillus cereus, bacillus brevis and bacillus pumilus; Phosphate solubilizing bacteria comprises: huge genus bacillus of phosphorus decomposing and pseudomonas fluorescens; The rhizosphere combination azotobacter comprises: klepsiella pneumoniae and enterobacter cloacae, in above-mentioned genus bacillus and phosphate solubilizing bacteria, rhizosphere combination azotobacter, but also each only select for use wherein a kind of, two or more are used in combination, the volume ratio of fermentation of bacillus liquid and phosphate solubilizing bacteria, rhizosphere association nitrogen fixation fermented liquid is followed successively by (2-3): (1-2): (3-5);
2) minimum plant element comprises at least: the compound of boron, zinc, copper, titanium, manganese, molybdenum, iodine, chlorine, potassium, silicon, iron, magnesium, can select sodium tetraborate 1-5% for use, zinc sulfate 1-4%, copper sulfate 0.01-0.06%, titanium dioxide 0.1-0.5%, potassiumiodide 0.05-0.5%, Repone K 1-5%, water glass 0.4-0.8%, sal epsom 0.005-0.02%, ferrous sulfate 0.02-0.08%, Sodium orthomolybdate 0.01-0.05%, wherein percentage ratio is all weight percentage;
3) sorbent material availablely comprises at least: the humic sour soil, turfy soil, sepiolite powder, vermiculite power or brown coal powder can only select for use wherein a kind of, two kinds or more of mixing to use, the granularity of described various materials is the 80-150 order, before using 90-120 ℃ of sterilization;
Above-mentioned 1) weight percent of the material-3) in described efficient biological compound fertilizer is followed successively by mixed fermentation liquid: the minimum plant element: the % of sorbent material=(26-38): (4-10) %: (60-70) %.
2. according to the described efficient biological compound fertilizer of claim 1, it is characterized in that, sorbent material in the described efficient biological compound fertilizer is that the per-cent of the two is 6: 4 through the humic sour soil powder of 90-120 ℃ of drying and sterilizing or the mixture of humic sour soil powder and sepiolite, and its granularity is the 80-150 order.
3. the preparation method of an efficient biological compound fertilizer is characterized in that the preparation method of described efficient biological compound fertilizer comprises at least:
One, microbial fermentation is cultivated:
(1) triangular flask is cultivated
Prepare the nutrition bouillon media of used various genus bacillus and phosphate solubilizing bacteria according to a conventional method and prepare each 2.5-4 liter of vinelandii substratum by following prescription (I), transfer PH6.8-7.2, be sub-packed in 500 milliliters the triangular flask the bottled 120-150 milliliter of each triangle, 0.1MPa sterilization 30 minutes.
Prescription (I) comprises at least: N.F,USP MANNITOL 5g, Sodium Glutamate 0.4g, Sunmorl N 60S 5g, potassium primary phosphate 0.8g, sodium-chlor 0.2g, sal epsom 0.2g, 1 milliliter of 1% Sodium orthomolybdate, 1 milliliter of 1% boric acid, 1 milliliter of 1% manganous sulfate, 1 milliliter in 1% ferrous sulfate, 1000 milliliters of distilled water are transferred PH6.8-7.2.
With genus bacillus, wherein available comprising: bacillus cereus, bacillus brevis and bacillus pumilus; Phosphate solubilizing bacteria comprises: huge genus bacillus of phosphorus decomposing and pseudomonas fluorescens, be seeded in the above-mentioned triangular flask that the nutrition bouillon media is housed, but each only selects for use wherein a kind ofly in above-mentioned genus bacillus and the phosphate solubilizing bacteria, also can select wherein two or more to be used in combination; Available rhizosphere combination azotobacter is klepsiella pneumoniae and enterobacter cloacae, also can only select one or both uses wherein for use, and it is seeded in the triangular flask that the vinelandii substratum is housed; On shaking table, keep 28-32 ℃ of temperature, shaking culture 2-4 days.
(2) seeding tank fermentation culture
(1). the preparation fermention medium
By following prescription (first), the substratum of preparation equivalent in A, B, C, D, E seed fermentation jar.In F, G seed fermentation jar, by the substratum of prescription (second) preparation equivalent.
Prescription (first): comprise at least: soyflour or soybean-cake flour 0.7-4%, Semen Maydis powder 0.5-2.5%, feed yeast powder 0.25-2.0% or yeast decoction 0.025-0.2%, dipotassium hydrogen phosphate 0.01-0.04%, sodium-chlor 0.25-0.6%, manganous sulfate 0.001-0.006%, other are water, transfer PH6.8-7.2,0.1MPa sterilization 30 minutes is used for the fermentation of genus bacillus and phosphate solubilizing bacteria;
Prescription (second): comprise at least: sucrose 0.5-2.0%, feed yeast powder 0.5-2.0% or yeast decoction 0.05-0.2%, Sunmorl N 60S 0.25-1.5%, potassium primary phosphate 0.05-0.15%, sodium-chlor 0.02-0.08%, sal epsom 0.02-0.08%, lime carbonate 0.2-0.4%, Sodium orthomolybdate 0.001-0.005%, boric acid 0.001-0.004%, manganous sulfate 0.001-0.003%, ferrous sulfate 0.001-0.003%, other are water, transfer PH6.8-7.2,0.1MPa sterilization 30 minutes;
(2). the seeding tank fermentation
When the substratum temperature in the above-mentioned seeding tank drops to 28-32 ℃, respectively the bacillus cereus in the triangular flask, bacillus brevis and bacillus pumilus are seeded in A, B, the C seed bottle, huge genus bacillus of phosphorus decomposing in the triangular flask and pseudomonas fluorescens are seeded in D, the E seed fermentation jar, klepsiella pneumoniae in the triangular flask and enterobacter cloacae are seeded in F, the G seeding tank, and inoculum size is (0.5-1.5) * 10
8Individual/the milliliter fermented liquid, control PH6.4-7.2, temperature 28-32 ℃, air flow 1: 0.25-1.0 treats that the bacterium number is (4-10) * 10 in A, B, C, D, the E fermentor tank
9Individual/milliliter, the bacterium number is (4-8) * 10 in F, the G fermentor tank
9Individual/when milliliter, stop fermentation.
(3) produce a jar fermentation
Except that the volume of producing jar and fermention medium amount of preparation enlarge 10 times than seeding tank, selected fermentative medium formula, the pH value of control and sterilising conditions are consistent with the seeding tank fermentation fully, when treating that each fermented liquid temperature in producing jar drops to 28-32 ℃, the corresponding one by one fermented liquid in the seeding tank is transferred to produced in the jar, enlarge fermented liquid and cultivate, control PH6.4-7.2, temperature 28-32 ℃, air flow 1: 0.25-1.0 treats that the bacterium number that A, B, C, D, E produce in the jar is (6-12) * 10
9Individual/milliliter, the bacterium number that F, G produce in the jar is (4-10) * 10
9During/milliliter, stop fermentation.
Two, be mixed and made into product
(1). from A, B, C, D, E, F, G produce jar, A: B: C: D: E: F: G=1 by volume successively: 1: 1: 1: emit fermented liquid in dosing vessel in be mixed into mixed fermentation liquid at 1: 2: 2;
(2). by weight 1 mixed fermentation liquid and minimum plant element and sorbent material are pressed (26-38) % successively: (4-10) %: (60-70) mixed of %, the minimum plant element comprises boron at least, zinc, copper, titanium, manganese, molybdenum, iodine, chlorine, potassium, silicon, the compound of iron and magnesium, can select sodium tetraborate 1-5% for use, zinc sulfate 1-4%, copper sulfate 0.01-0.06%, titanium dioxide 0.1-0.5%, potassiumiodide 0.05-0.5%, Repone K 1-5%, water glass 0.4-0.8%, sal epsom 0.005-0.02%, ferrous sulfate 0.02-0.08%, Sodium orthomolybdate 0.01-0.05%, wherein percentage ratio is all weight percentage; Sorbent material availablely comprises at least: the humic sour soil, turfy soil, sepiolite powder, vermiculite power or brown coal powder can only select for use wherein a kind of, two kinds or more of mixing to use, the granularity of described various materials is the 80-150 order, before using 90-120 ℃ of sterilization;
(3). packing: after above-mentioned various materials mixing, be sub-packed in the sealing bag, also can be minimum plant element and the sorbent material that has adsorbed mixed bacteria liquid to be separated packing in proportion use, in the efficient biological compound fertilizer of the present invention, total living bacteria count be (8-25) * 10
8Individual/gram.
4. according to the preparation method of the described efficient biological compound fertilizer of claim 3, it is characterized in that, the genus bacillus of using in the described method is bacillus cereus, bacillus brevis and bacillus pumilus, and phosphate solubilizing bacteria is huge genus bacillus of phosphorus decomposing and pseudomonas fluorescens; The substratum of this two classes bacterium in fermentation is for containing soyflour 2.5%, Semen Maydis powder 0.75%, feed yeast powder 0.75%, dipotassium hydrogen phosphate 0.2%, sodium-chlor 0.5%, manganous sulfate 0.02%, the aqueous solution of PH6.4-7.2; Rhizosphere association nitrogen fixation bacterium culture medium is a sucrose 1.0%, soyflour 1.5%, feed yeast powder 0.75, Sunmorl N 60S 0.5%, Sodium Glutamate 0.1%, potassium primary phosphate 0.08%, sodium-chlor 0.025%, sal epsom 0.05%, Sodium orthomolybdate 0.001%, boric acid 0.001%, manganous sulfate 0.001%, ferrous sulfate 0.002%, the aqueous solution of PH6.4-7.2;
After fermentation stops, with 7 kinds of fermented liquids 1: 1: 1 by volume: 1: 1: 2: 2 mixed, and again the blended fermented liquid are mixed the mixed solution that promptly ferments by following weight ratio: the minimum plant element: sorbent material=(26-38): (4-10): (60-70);
Minimum plant element wherein comprises at least: sodium tetraborate 2.7-3.5%, zinc sulfate 1.5-3.7%, copper sulfate 0.01-0.05%, titanium dioxide 0.15-0.5%, potassiumiodide 0.1-0.5%, ferrous sulfate 0.03-0.05%, Repone K 1.5-5%, water glass 0.4-0.7%, sal epsom 0.005-0.015%, Sodium orthomolybdate 0.01-0.04%, sorbent material wherein are 60% humic sour soil and 40% sepiolite uniform mixture, through the powder of 90-120 ℃ of sterilization, granularity is the 80-150 order.
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|---|---|---|---|
| CN 96109854 CN1150133A (en) | 1996-09-20 | 1996-09-20 | Efficient biological compound fertilizer and its preparing process |
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| CN 96109854 CN1150133A (en) | 1996-09-20 | 1996-09-20 | Efficient biological compound fertilizer and its preparing process |
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Cited By (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061684C (en) * | 1997-08-01 | 2001-02-07 | 上海农乐生物制品股份有限公司 | Anti-disease synergic bacteria and culture method thereof |
| CN1078192C (en) * | 1999-02-14 | 2002-01-23 | 王景迢 | Bio-bacterial agent and its preparing process |
| WO2010109436A1 (en) * | 2009-03-25 | 2010-09-30 | Carepro Bioscience (P) Ltd | Microbial formulation for widespread uesd in agricultural practices |
| CN101289338B (en) * | 2008-05-28 | 2010-12-01 | 杜清华 | Biological salt-decomposition bacterial manure and method for preparing same |
| CN101585736B (en) * | 2009-07-03 | 2012-07-04 | 嵇书琼 | Agricultural composite microbial preparation and preparing method thereof |
| CN103772011A (en) * | 2013-12-27 | 2014-05-07 | 天津北洋百川生物技术有限公司 | Special compound biological liquid-state bacterial fertilizer for grain |
| CN103898019A (en) * | 2014-03-29 | 2014-07-02 | 山东金利丰生物科技股份有限公司 | Continuous cropping resistant composite microbial agent and preparation method thereof |
| CN103965916A (en) * | 2014-05-07 | 2014-08-06 | 苟俊 | Soil restoration agent, method for preparing soil restoration agent and method for restoring soil |
| CN104447054A (en) * | 2014-12-21 | 2015-03-25 | 竹溪县宝田生物制品有限公司 | Special biological organic fertilizer for tea |
| CN104478616A (en) * | 2014-12-29 | 2015-04-01 | 安徽飞天农用生物科技股份有限公司 | High-content inorganic biological bacterial fertilizer and preparation method thereof |
| CN104725141A (en) * | 2015-03-25 | 2015-06-24 | 青岛海力源生物科技有限公司 | Humic acid fertilizer synergist with ammonia volatilization inhibition effect and preparation method thereof |
| CN104744133A (en) * | 2015-03-03 | 2015-07-01 | 中国烟草总公司广东省公司 | Bioactive organic fertilizer special for soybeans and application of bioactive organic fertilizer |
| CN105001021A (en) * | 2015-08-04 | 2015-10-28 | 沈阳鑫嘉捷生物制剂有限公司 | Total-element compound microorganism organic fertilizer and preparing technology thereof |
| CN105693418A (en) * | 2016-04-22 | 2016-06-22 | 广西喷施宝股份有限公司 | Bioremediation soil conditioner and preparation method thereof |
| CN106007830A (en) * | 2016-05-16 | 2016-10-12 | 江苏省家禽科学研究所 | Manure fermenting compound bacterium solution and application thereof |
| CN106190146A (en) * | 2016-07-12 | 2016-12-07 | 山东胜伟园林科技有限公司 | A kind of salt-soda soil renovation agent for Fructus Mori plantation and preparation method thereof |
| CN106810370A (en) * | 2017-02-22 | 2017-06-09 | 广州聚禅现代农业研究院有限公司 | A kind of microbial manure containing chelated microelements and preparation method thereof |
| CN107141061A (en) * | 2017-05-04 | 2017-09-08 | 秦勇义 | The double micro- suit composite fertilizers of micro- microbial bacteria |
| CN108395893A (en) * | 2017-02-08 | 2018-08-14 | 山东中创亿丰肥料集团有限公司 | A kind of improvement soil complex micro organism fungicide and preparation method thereof |
| PH12017000199A1 (en) * | 2017-07-07 | 2019-01-21 | Univ Of The Philippines Los Banos | Microbial-based foliar fertilizer |
| CN109608283A (en) * | 2018-12-26 | 2019-04-12 | 龙蟒大地农业有限公司 | A kind of biological compound fertilizer |
| CN110229767A (en) * | 2019-06-19 | 2019-09-13 | 福建师范大学福清分校 | Bacillus pumilus S1420 and its application in Soluble phosphorus |
| CN110521735A (en) * | 2018-11-26 | 2019-12-03 | 甘肃省农业科学院植物保护研究所 | Dedicated root rot prevention and treatment microbial inoculum of a kind of highland barley and preparation method thereof |
| CN114907991A (en) * | 2022-05-09 | 2022-08-16 | 漳州三炬生物技术有限公司 | Compound microorganism for decomposing banana straws, banana straw decomposing agent, banana straw organic fertilizer and application |
| CN115362924A (en) * | 2022-08-22 | 2022-11-22 | 江西省中国科学院庐山植物园 | Method for promoting aquatic plant to take root in gravel environment |
| WO2024058727A1 (en) * | 2021-08-27 | 2024-03-21 | Atatürk Üni̇versi̇tesi̇ Rektörlüğü Bi̇li̇msel Araştirma Projeleri̇ ( Bap ) Koordi̇nasyon Bi̇ri̇mi̇ | Solid carrier microbial fertilizer and biopesticide formulation |
-
1996
- 1996-09-20 CN CN 96109854 patent/CN1150133A/en not_active Withdrawn
Cited By (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1061684C (en) * | 1997-08-01 | 2001-02-07 | 上海农乐生物制品股份有限公司 | Anti-disease synergic bacteria and culture method thereof |
| CN1078192C (en) * | 1999-02-14 | 2002-01-23 | 王景迢 | Bio-bacterial agent and its preparing process |
| CN101289338B (en) * | 2008-05-28 | 2010-12-01 | 杜清华 | Biological salt-decomposition bacterial manure and method for preparing same |
| WO2010109436A1 (en) * | 2009-03-25 | 2010-09-30 | Carepro Bioscience (P) Ltd | Microbial formulation for widespread uesd in agricultural practices |
| CN101585736B (en) * | 2009-07-03 | 2012-07-04 | 嵇书琼 | Agricultural composite microbial preparation and preparing method thereof |
| CN103772011B (en) * | 2013-12-27 | 2016-04-20 | 天津北洋百川生物技术有限公司 | The liquid bacterial manure of a kind of cereal special composite biological |
| CN103772011A (en) * | 2013-12-27 | 2014-05-07 | 天津北洋百川生物技术有限公司 | Special compound biological liquid-state bacterial fertilizer for grain |
| CN103898019A (en) * | 2014-03-29 | 2014-07-02 | 山东金利丰生物科技股份有限公司 | Continuous cropping resistant composite microbial agent and preparation method thereof |
| CN103965916A (en) * | 2014-05-07 | 2014-08-06 | 苟俊 | Soil restoration agent, method for preparing soil restoration agent and method for restoring soil |
| CN104447054A (en) * | 2014-12-21 | 2015-03-25 | 竹溪县宝田生物制品有限公司 | Special biological organic fertilizer for tea |
| CN104478616A (en) * | 2014-12-29 | 2015-04-01 | 安徽飞天农用生物科技股份有限公司 | High-content inorganic biological bacterial fertilizer and preparation method thereof |
| CN104744133A (en) * | 2015-03-03 | 2015-07-01 | 中国烟草总公司广东省公司 | Bioactive organic fertilizer special for soybeans and application of bioactive organic fertilizer |
| CN104725141A (en) * | 2015-03-25 | 2015-06-24 | 青岛海力源生物科技有限公司 | Humic acid fertilizer synergist with ammonia volatilization inhibition effect and preparation method thereof |
| CN105001021A (en) * | 2015-08-04 | 2015-10-28 | 沈阳鑫嘉捷生物制剂有限公司 | Total-element compound microorganism organic fertilizer and preparing technology thereof |
| CN105693418A (en) * | 2016-04-22 | 2016-06-22 | 广西喷施宝股份有限公司 | Bioremediation soil conditioner and preparation method thereof |
| CN106007830A (en) * | 2016-05-16 | 2016-10-12 | 江苏省家禽科学研究所 | Manure fermenting compound bacterium solution and application thereof |
| CN106190146A (en) * | 2016-07-12 | 2016-12-07 | 山东胜伟园林科技有限公司 | A kind of salt-soda soil renovation agent for Fructus Mori plantation and preparation method thereof |
| CN108395893A (en) * | 2017-02-08 | 2018-08-14 | 山东中创亿丰肥料集团有限公司 | A kind of improvement soil complex micro organism fungicide and preparation method thereof |
| CN106810370A (en) * | 2017-02-22 | 2017-06-09 | 广州聚禅现代农业研究院有限公司 | A kind of microbial manure containing chelated microelements and preparation method thereof |
| CN107141061A (en) * | 2017-05-04 | 2017-09-08 | 秦勇义 | The double micro- suit composite fertilizers of micro- microbial bacteria |
| PH12017000199A1 (en) * | 2017-07-07 | 2019-01-21 | Univ Of The Philippines Los Banos | Microbial-based foliar fertilizer |
| CN110521735A (en) * | 2018-11-26 | 2019-12-03 | 甘肃省农业科学院植物保护研究所 | Dedicated root rot prevention and treatment microbial inoculum of a kind of highland barley and preparation method thereof |
| CN109608283A (en) * | 2018-12-26 | 2019-04-12 | 龙蟒大地农业有限公司 | A kind of biological compound fertilizer |
| CN110229767A (en) * | 2019-06-19 | 2019-09-13 | 福建师范大学福清分校 | Bacillus pumilus S1420 and its application in Soluble phosphorus |
| WO2024058727A1 (en) * | 2021-08-27 | 2024-03-21 | Atatürk Üni̇versi̇tesi̇ Rektörlüğü Bi̇li̇msel Araştirma Projeleri̇ ( Bap ) Koordi̇nasyon Bi̇ri̇mi̇ | Solid carrier microbial fertilizer and biopesticide formulation |
| CN114907991A (en) * | 2022-05-09 | 2022-08-16 | 漳州三炬生物技术有限公司 | Compound microorganism for decomposing banana straws, banana straw decomposing agent, banana straw organic fertilizer and application |
| CN114907991B (en) * | 2022-05-09 | 2024-03-29 | 漳州三炬生物技术有限公司 | Composite microorganism for decomposing banana straw, banana straw decomposing agent, banana straw organic fertilizer and application |
| CN115362924A (en) * | 2022-08-22 | 2022-11-22 | 江西省中国科学院庐山植物园 | Method for promoting aquatic plant to take root in gravel environment |
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