CN103772011A - Special compound biological liquid-state bacterial fertilizer for grain - Google Patents
Special compound biological liquid-state bacterial fertilizer for grain Download PDFInfo
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- CN103772011A CN103772011A CN201310747136.4A CN201310747136A CN103772011A CN 103772011 A CN103772011 A CN 103772011A CN 201310747136 A CN201310747136 A CN 201310747136A CN 103772011 A CN103772011 A CN 103772011A
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Abstract
The invention discloses a special compound biological liquid-state bacterial fertilizer for grain, relating to the preparation of a special compound biological liquid-state bacterial fertilizer suitable for grain. The nutritional ingredients for the growth cycle of the grain are reasonably combined, and solid powder and fermentation strain liquid are mixed under aseptic conditions to obtain the special compound biological liquid-state bacterial fertilizer for grain. Long-time supply of the fertilizer can be prolonged and maintained through the sustained release of microorganisms. The special compound biological liquid-state bacterial fertilizer for grain can effectively prevent pest and disease damage, improve the soil components, enhance the resistance of crops, fix carbon dioxide, improve the soil fertility and stimulate the crop growth, and is widely applied to grain crops.
Description
Technical field
The invention belongs to field of compound fertilizer production, be specifically related to the preparation of the liquid bacterial manure of a kind of applicable cereal special composite biological.
Background technology
Common fertilizer is for cereals crop, and fertilizer efficiency period is short, and nutrient proportioning is unreasonable, supplies with not science, more unbalanced.Long-term application chemical fertilizer, can not effectively provide nutritive substance to crop, also can cause the loss of main component, cause the huge waste of resource and the increase of agricultural cost, produce the pollution in underground water and soil face source simultaneously, thereby the eutrophication that causes greenhouse gases effect and water body, makes the deterioration of the ecological environment.Under such condition, carry out the reasonably combined of nutritive ingredient in the urgent need to the growth cycle for cereal, then extend and keep the long-term lasting supply of fertilizer by the slowly-releasing of microorganism.
Cereals mainly refer to the seed of grass.Five cereals mainly comprise rice, wheat, corn etc. and other coarse cereals, such as millet, black rice, buckwheat, oat, seed of Job's tears, Chinese sorghum etc.Cereal is by being processed as staple food, for the mankind provide 50%～80% heat energy, 40%～70% protein, more than 60% VITMAIN B1.Cereal can be because of the difference of kind, kind, the place of production, growth conditions and working method, and the content of its nutrient substance has very large difference.
Cereals, as Chinese traditional food, are one of indispensable food on common people's dining table for thousands of years always, in the meals of China, occupy an important position, and are taken as traditional staple food.Paddy belongs to the common rice subspecies in Gramineae Oryza common cultivated rice subgenus in phytology.One of topmost food crop of Ta Shi China, at present, the sown area of China paddy rice accounts for 1/4 of the food crop total area, and output accounts for 1/2 of national total output of grain, accounts for over half in commodity food.Producing region spreads all over all parts of the country.Common cultivated rice can be divided into long-grained nonglutinous rice and two subspecies of japonica rice.Long-grained nonglutinous rice particle shape is elongated, and length is the more than three times of width, and flat, fine hair is short and rare, and generally without awns, even if there is awns also very short, rice husk is thinner, and white belly is larger, and hard grain is less, adds and easily goes out to crack rice man-hour, and rice milling yield is lower, rice matter swollen property compared with large and viscosity a little less than.Particle shape is short cuts for japonica rice, length be width 1.4-2.9 doubly, fine hair is long and close, awns is longer, rice husk is thicker, white belly is little or do not have, hard grain is many, adds and is difficult for man-hour producing cracking rice, rice milling yield is higher, the swollen property of rice matter is compared with little and viscosity is stronger.Long-grained nonglutinous rice or japonica rice according to the difference of length in its, can be divided into again early rice, semilate rice and late rice three classes in vegetative period.The 90-125 days in vegetative period of early rice, be 125-150 days the vegetative period of semilate rice, be 150-180 days the vegetative period of late rice.Due to the difference of vegetative period length and weather condition, the quality of the paddy of same type also shows some difference: early the general white belly of rice is larger, and hard grain is less, and rice matter is loose, inferior quality, on the contrary late rice rice is, and quality is better.Wheat is a kind of temperate zone long day plant, and subject range is wider, and from 18 °～50 ° of north latitude, from Plain to height above sea level, the height of 4000 meters (as Tibet, China) all has cultivation.According to the spicate density degree of wheat, the structure of small ear, the proterties of lepicena, lemma and awns and grain, color, lint etc., be divided into extremely many subspecies, mutation, modification and kind under kind; According to the requirement difference to temperature, point winter wheat and two bions of spring wheat, different areas plantation is dissimilar.In China Dark Longjiang, the Inner Mongol and northwest plant spring wheat, in sowing in 3～April of spring, 7～August maturation, breeding time is short, about approximately 100 days; In the east area of the Liao River, coextensive with eastern and southern Liaoning Province, North China, southern Xinjiang, Shaanxi, Yangtze valley each province and south China one band plantation winter wheat, sowing in 8～December of autumn, 5～July of next year, maturation, reached about 300 days breeding time.The main planting system of corn: northeast, North of North China and Counties of North-west Five area are 1 year ripe Spring Maize Area; The North China Plain is take 1 year two ripe summer corn as main; Southwest and southern hills alpine terrain complexity, high and cold mountain area is with 1 year ripe spring maize, Hills with 1 year two ripe summer corn, and He Qian mountain area, Plain is take 1 year three ripe autumn corn as main.Planting type has nonoculture, or with beans, the intercropping of potato class and Mai Long interplanting etc.Sowing date for early sowing is very important to its volume increase.According to fertilizer, water condition and breediness, rational close planting, guarantees that enough seedling numbers and spike number are the keys of high yield.Corn needs nitrogen, potassium more, need phosphorus less, and can utilize first crop phosphoric residue element, but soil available phosphorus amount is during lower than 10ppm, executes phosphorus and significantly increases production.To zinc fertilizer sensitivity, while lacking zinc, grow suppressed.5600～6100 millimeters/hectare of full fertility water requirements.Need seedling stage water less, jointing-booting stage increases, and takes out hero maximum flowering period, and the grouting ripening stage is more.
Summary of the invention
The object of this invention is to provide the production technique of the liquid bacterial manure of a kind of cereal special composite biological, carry out reasonably combinedly for the nutritive ingredient in corn growing cycle, extend and keep the long-term of fertilizer to supply lastingly by the slowly-releasing of microorganism.
The present invention adopts following technical scheme:
Material content of the present invention (calculating by weight) is as follows:
Concrete technical process is as follows:
(1) under normal temperature aseptic condition, a certain amount of pressed powder is added in tempering tank and stirred in proportion.
(2) under normal temperature aseptic condition, by four kinds of bacteria containing amounts all 10
8individual/more than ml fermented bacterium liquid adds mixing and stirring in another tempering tank by a certain percentage, as the solvent of pressed powder raw material.
(3) the pressed powder screw feeder mixing in (1) step is delivered in the tempering tank that bacterium liquid is housed, mixing and stirring, by the storage of mixed sample sealing normal temperature lucifuge.
Described bacillus thuringiensis, bacillus megaterium, Pseudomonas fluorescens and Jingyang streptomycete are to obtain zymocyte liquid after fermentation culture.
Beneficial effect
The present invention utilizes the material actings in conjunction such as microorganism, inorganic salt, organism, can long-lastingly provide nutritive substance to reach to cereal.Meticulously select suitable microorganism, one of its bacterial classification: bacillus thuringiensis is a kind of microbial source low toxic pesticide, take stomach poison function as main, can effectively preventing insect pest, and to people and animals without action effect.Two of bacterial classification: bacillus megaterium, can self phosphorus decomposing fixed nitrogen, organophosphorus in the soil of can well degrading, for plant provides essential nutrition nutrient, improves soil constituent.Three of bacterial classification: colloid bacillus cereus, claim again silicate bacteria, can secrete plant growth substance and plurality of enzymes, to strengthen the resistibility of crop to some diseases.Promote the conversion of the invalid phosphorus potassium of soil, increase the supply of soil phosphorus potassium, improve crop yield, the intensity of colloid bacillus cereus phosphorus decomposing, potassium decomposing, solution silicon is very good especially simultaneously.Colloid bacillus cereus also can produce carbonic anhydrase, and the fixing tool of carbonic acid gas is had certain effect.Four of bacterial classification: Jingyang streptomycete, can suppress some fungal diseases.There is the ability that strengthens soil fertility, stimulates plant growth.By their different-effect is reasonably adjusted to proportioning, between each bacterial classification, act synergistically, the function of bacterial classification is farthest brought into play, to be applicable to the demand of corn growing.
Embodiment
Cereal composite biological fertilizer specially formula (calculate by weight unit: part) as follows:
| Raw material | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 |
| The fertile EDTA-Ca of Ca | 6.2 | 6.5 | 6.8 | 7.2 |
| The fertile EDTA-Mn of Mn | 0.15 | 0.18 | 0.21 | 0.25 |
| The fertile EDTA-Cu of Cu | 0.015 | 0.017 | 0.019 | 0.02 |
| The fertile EDTA-Zn of Zn | 0.042 | 0.045 | 0.048 | 0.051 |
| The fertile EDTA-Mg of Mg | 7.25 | 7.45 | 7.85 | 8.32 |
| The fertile EDTA-Fe of Fe | 0.15 | 0.18 | 0.21 | 0.24 |
| Zinc Sulphate Heptahydrate | 0.028 | 0.03 | 0.032 | 0.035 |
| Magnesium sulfate heptahydrate | 4.5 | 4.8 | 5.0 | 5.2 |
| Manganese sulfate monohydrate | 0028 | 003 | 0032 | 0035 |
| Cupric sulfate pentahydrate | 0.018 | 0.02 | 0.022 | 0.025 |
| Boric acid | 0.045 | 0.048 | 0.05 | 0.052 |
| Sodium alginate | 0012 | 0016 | 002 | 0025 |
| Ammonium molybdate | 0.022 | 0.023 | 0.026 | 0.028 |
| Potassium primary phosphate | 72 | 73 | 74 | 75 |
| Humic acids | 0.22 | 0.24 | 0.26 | 0.28 |
| Soybean protein | 1.15 | 1.28 | 1.4 | 1.5 |
| Asparagus juice | 2.25 | 2.3 | 2.4 | 2.5 |
| Seaweed powder | 0.32 | 0.38 | 0.46 | 0.55 |
| Water glass | 0.022 | 0.026 | 0.03 | 0.035 |
| Anhydrous slufuric acid ferrous iron | 0.12 | 0.14 | 0.16 | 0.18 |
| Bacillus thuringiensis | 43.2 | 45.5 | 47.5 | 48.6 |
| Bacillus megaterium | 22.5 | 23.1 | 23.7 | 24.3 |
| Colloid bacillus cereus | 22.5 | 23.1 | 23.7 | 24.3 |
| Jingyang streptomycete | 2.83 | 3.5 | 4.2 | 4.82 |
Wherein, bacterial classification of the present invention is as follows:
(1) bacillus thuringiensis: bacillus thuringiensis (Bacillus thuringiensis is called for short Bt) is current production rate maximum, the widest biotic pesticide of use.To insects such as lepidopteran, Coleoptera, Diptera, Hymenoptera, Homopteras, and the arthropods such as animals and plants nematode, acarian has specific cytotoxicity, and to nontarget organism safety therefore, has single-minded, efficient and to advantages such as person poultry safeties.Culture presevation numbering: microbial strains preservation center, Guangdong Province, numbering GIM1.151.
(2) bacillus megaterium: bacillus megaterium has effect of organophosphorus in good degraded soil, is the conventional bacterial classification of widely used production biological organic fertilizer in the world, and it is administered on tobacco leaf improving tobacco fermentation flavouring effect uniqueness.Culture presevation numbering: Chinese common micro-organisms culture presevation administrative center, numbering CGMCC1.223.
(3) colloid bacillus cereus: colloid bacillus cereus claims again silicate bacteria, its key property is potassium, the silicon that can decomposite in the mineral such as feldspar, mica, also can decomposite the phosphorus in phosphatic rock, and secretion plant growth substance and plurality of enzymes, to strengthen the resistibility of crop to some diseases, be the best bacterial classification that is used as at present bio-feritlizer, can promote the conversion of the invalid phosphorus potassium of soil, increase the supply of soil phosphorus potassium, improve crop yield.It can produce carbonic anhydrase simultaneously, and the fixing tool of carbonic acid gas is had certain effect.Culture presevation numbering: Chinese industrial microbial strains preservation administrative center, numbering CICC23575.
(4) Jingyang streptomycete: can suppress some fungal diseases.There is the ability that strengthens soil fertility, stimulates plant growth.Culture presevation numbering: microbial strains preservation center, Guangdong Province, numbering GIM4.92.
Embodiment 1
(1) under normal temperature aseptic condition, pressed powder is added in tempering tank and stirred in proportion.
(2) under normal temperature aseptic condition, by four kinds of bacteria containing amounts all 10
8individual/more than ml fermented bacterium liquid proportional adds mixing and stirring in another tempering tank, as the solvent of pressed powder raw material.
(3) the pressed powder screw feeder mixing in (1) step is delivered in the tempering tank that bacterium liquid is housed, mixing and stirring, by the storage of mixed sample sealing normal temperature lucifuge.
Embodiment 2
(1) under normal temperature aseptic condition, pressed powder is added in tempering tank and stirred in proportion.
(2) under normal temperature aseptic condition, by four kinds of bacteria containing amounts all 10
8individual/more than ml fermented bacterium liquid proportional adds mixing and stirring in another tempering tank, as the solvent of pressed powder raw material.
(3) the pressed powder screw feeder mixing in (1) step is delivered in the tempering tank that bacterium liquid is housed, mixing and stirring, by the storage of mixed sample sealing normal temperature lucifuge.
Embodiment 3
(1) under normal temperature aseptic condition, pressed powder is added in tempering tank and stirred in proportion.
(2) under normal temperature aseptic condition, by four kinds of bacteria containing amounts all 10
8individual/more than ml fermented bacterium liquid proportional adds mixing and stirring in another tempering tank, as the solvent of pressed powder raw material.
(3) the pressed powder screw feeder mixing in (1) step is delivered in the tempering tank that bacterium liquid is housed, mixing and stirring, by the storage of mixed sample sealing normal temperature lucifuge.
Embodiment 4
(1) under normal temperature aseptic condition, pressed powder is added in tempering tank and stirred in proportion.
(2) under normal temperature aseptic condition, by four kinds of bacteria containing amounts all 10
8individual/more than ml fermented bacterium liquid proportional adds mixing and stirring in another tempering tank, as the solvent of pressed powder raw material.
(3) the pressed powder screw feeder mixing in (1) step is delivered in the tempering tank that bacterium liquid is housed, mixing and stirring, by the storage of mixed sample sealing normal temperature lucifuge.
Field experiment
The liquid bacterial manure of cereal special composite biological and common fertilising crop prepared by the present invention contrast, crop yield successful, and concrete testing program and result are as follows:
Embodiment 1 gets composite biological fertilizer 2ml of the present invention, dilute 500 times, spray on the millet seedling just having sprouted, every square meter sprays the composite bio-fertilizer of the present invention of 1～1.5L amount, treats that stem and leaf of Wheat grows to 10～15cm, refertilizes once, this time every square meter sprays the composite fertilizer of 2～2.5L amount, then while waiting wheat heading, execute last fertilizer, every square meter fertilising 1～1.5L amount, this time adopts the mode of filling with root to apply fertilizer to it.With the stem and leaf of Wheat ratio of Wei Shigai composite fertilizer, in whole growth cycle, the seedling of fertilising is more sturdy than common, and color is greener, and heading period has shifted to an earlier date 2～3 days, and average every square meter can improve 8% effect of increasing production.
Embodiment 2 gets composite biological fertilizer 2ml of the present invention, dilute 500 times, spray on the maize seedling just having sprouted, every square meter sprays the composite bio-fertilizer of the present invention of 1～1.5L amount, treats that maize seedling grows to 30～35cm, refertilizes once, this time every square meter sprays the composite fertilizer of 2～2.5L amount, then while waiting corn heading, execute last fertilizer, every square meter fertilising 1～1.5L amount, adopts the mode of filling with root.With the maize seedling ratio of Wei Shigai composite fertilizer, in whole growth cycle, the seedling of fertilising is more solid than common seedling stalk, color is greener, the fruit of knot is than common length 2～3cm, and growth cycle has shortened 6～8 days than unmanured, and the average every square meter of fruit harvest can improve 10% effect of increasing production.
Embodiment 3 gets composite biological fertilizer 2ml of the present invention, dilute 500 times, spray on the oat seedling just having sprouted, every square meter sprays the composite bio-fertilizer of the present invention of 1～1.5L amount, treats that oat seedling grows to 20～325cm, refertilizes once, this time every square meter sprays the composite fertilizer of 2～2.5L amount, then while waiting oat to start to ear, execute last fertilizer, every square meter fertilising 1～1.5L amount, adopts the root mode of filling with.With the oat seedling ratio of not executing this composite bio-fertilizer, in whole growth cycle, the seedling of fertilising is more solid than common seedling stalk, 5～6 wheat heads of the unmanured many heading of every young plant average specific, and fruit is fuller, and every square meter output has improved 11% effect.
Claims (8)
1. the liquid bacterial manure of cereal special composite biological, is characterized in that, formula is counted by weight:
2. the liquid bacterial manure of cereal composite fertilizer special according to claim 1, is characterized in that, adopts following formula to count by weight:
3. the liquid bacterial manure of cereal composite fertilizer special according to claim 1, is characterized in that bacterial content>=10 in described each bacterium bacterium liquid
8individual/ml.
4. the liquid bacterial manure of cereal composite fertilizer special according to claim 1, is characterized in that, described bacillus thuringiensis is numbered GIM1.151.
5. the liquid bacterial manure of cereal composite fertilizer special according to claim 1, is characterized in that, bacillus megaterium is CGMCC1.223.
6. the liquid bacterial manure of cereal composite fertilizer special according to claim 1, is characterized in that, colloid bacillus cereus numbering CICC23575.
7. the liquid bacterial manure of cereal composite fertilizer special according to claim 1, is characterized in that, Jingyang streptomycete numbering GIM4.92.
8. the using method of the liquid bacterial manure of a kind of cereal special composite biological according to claim 1, is characterized in that:
Get composite biological fertilizer 2ml of the present invention, dilute 500 times, spray on the oat seedling just having sprouted, every square meter sprays the composite bio-fertilizer of the present invention of 1～1.5L amount, treats that oat seedling grows to 20～325cm, refertilizes once, this time every square meter sprays the composite fertilizer of 2～2.5L amount, then while waiting oat to start to ear, execute last fertilizer, every square meter fertilising 1～1.5L amount, adopts the root mode of filling with.
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Cited By (8)
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| CN104478525A (en) * | 2014-12-25 | 2015-04-01 | 京博农化科技股份有限公司 | Microelement effervescent granule for plant nutrition |
| CN105061093A (en) * | 2015-09-09 | 2015-11-18 | 山东乡约生物工程科技有限公司 | Phytochelatin microbial agent and preparation method thereof |
| CN105481548A (en) * | 2015-12-11 | 2016-04-13 | 天津北洋百川生物技术有限公司 | Flower special-purpose composite biological fertilizer, and preparation method thereof |
| CN106699416A (en) * | 2017-02-23 | 2017-05-24 | 胡世洋 | Multielement liquid compound microorganism fertilizer and preparation method thereof |
| CN107056421A (en) * | 2017-03-01 | 2017-08-18 | 五常市盛世兴邦农业科技有限公司 | Biological organic foliar fertilizer |
| CN107827627A (en) * | 2017-12-26 | 2018-03-23 | 天津北洋百川生物技术有限公司 | A kind of strawberry special composite biological fertilizer and its preparation method and application |
| CN108401636A (en) * | 2018-04-19 | 2018-08-17 | 中国农业大学 | A kind of high-yield fertilization method of organic oat |
| CN110622795A (en) * | 2019-09-27 | 2019-12-31 | 贵州省亚热带作物研究所 | Good agricultural operation management method for coix |
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| CN110622795A (en) * | 2019-09-27 | 2019-12-31 | 贵州省亚热带作物研究所 | Good agricultural operation management method for coix |
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| CN103772011B (en) | 2016-04-20 |
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Effective date of registration: 20190507 Address after: 410300 Jinwan Formation, Nanyue Village, Guandu Town, Liuyang City, Changsha City, Hunan Province Patentee after: Hunan Xinyu Biotechnology Co., Ltd. Address before: No. 29 Xiangan Road, Economic and Technological Development Zone, Binhai New Area, Tianjin City, 300457 Patentee before: Tianjin Peiyang Biotrans Biotech Co., Ltd. |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160420 Termination date: 20191227 |