CN105061093A - Phytochelatin microbial agent and preparation method thereof - Google Patents
Phytochelatin microbial agent and preparation method thereof Download PDFInfo
- Publication number
- CN105061093A CN105061093A CN201510568818.8A CN201510568818A CN105061093A CN 105061093 A CN105061093 A CN 105061093A CN 201510568818 A CN201510568818 A CN 201510568818A CN 105061093 A CN105061093 A CN 105061093A
- Authority
- CN
- China
- Prior art keywords
- phytochelatin
- parts
- content
- microbiobacterial agent
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a phytochelatin microbial agent and a preparation method thereof. The microbial agent comprises plant active peptides, bacillus natto bacterial liquid, pichia pastoris bacterial liquid, lactobacillus casei bacterial liquid, erycibe obtusifolia benth extract, ulva powder, gallnut powder, ferrous sulfate, potassium fulvic acid and a chelating agent component. In the bacillus natto bacterial liquid, the content of bacillus natto is 40*10<9>CPU/ml; in the pichia pastoris bacterial liquid, the content of pichia pastoris is 16*10<9>CPU/ml; in the lactobacillus casei bacterial liquid, the content of lactobacillus casei is 22*10<9>CPU/ml; in the chelating agent is a mixture consisting of EDTA and calcium superphosphate at a weight ratio of 3.5:1. The content of functional bacterium in the microbial agent is (4.8 to 5.2)*10<9>/g, the content of trace elements is 42 to 45 mg/g, and the infectious microbe rate is 4.5 to 5.2 percent.
Description
Technical field
The invention belongs to microbial fertilizer field, relate to a kind of phytochelatin microbiobacterial agent and preparation method thereof.
Background technology
Microbiobacterial agent is that continuous cropping continuous cropping increases for arable farming and growing and transplanting seedling enlarged areas, the situation that diseases and pests of agronomic crop is on the rise, and adopts the disease-resistant bacterial manure of new bio that High biotechnology develops.
Beneficial microorganism, in breeding metabolic process, secretory cell mitogen, Yin can stamp plant hormone and the amino acid isoreactivity materials such as acetic acid, VITAMIN, this acetic acid, Plant hormones regulators,gibberellins, and powerfully promote extraneous root, take root, strong root, capillary radical amount double above.Water suction, fertilizer sucting ability are strengthened, and stem is thick, the features such as seedling is fast are delayed in Miao Zhuan, transplanting.
Although have many microbiobacterial agents on the market, these microbiobacterial agents, have many defects and problem more, in fertilizer, the bacteria containing amount of active bacteria is low, poor to the rejection ability of miscellaneous bacteria, and miscellaneous bacteria content is higher; Along with the prolongation in storage time, the activity of probiotics is restricted, probiotics cannot in fertilizer survive, living bacteria count can reduce day by day, miscellaneous bacteria is increasing on the contrary, very not for shelf-stable, the microbiobacterial agent failed, effect after using is had a greatly reduced quality, and does not even have corresponding effect.
In a word, there is following defect in existing microbiobacterial agent: (1) effectively viable bacteria content is low, miscellaneous bacteria rate is high; (2) not resistance to storage, effective storage time is short; (3) rear effect is used undesirable.
Summary of the invention
The present invention is directed to above deficiency, a kind of phytochelatin microbiobacterial agent and preparation method thereof be provided, realize following goal of the invention:
1, improve effective bacterial content of microbiobacterial agent, improve micronutrient levels, reduce miscellaneous bacteria rate; Effective bacterial content is made to be 4.8-5.2 × 10
9individual/g, micronutrient levels is 42-45mg/g, and miscellaneous bacteria rate is 4.5-5.2%;
2, improve the microorganism active degree of stability of microbiobacterial agent, extend effective storage time; Make storage after 980 days, effective bacterial content is 5.2-5.8 × 10
9individual/g, miscellaneous bacteria rate is 4.7-5.7%;
3, improve the application effect of microbiobacterial agent, improve the seed output and quality of peanut cultivation; Improve growing area edatope.
for solving the problems of the technologies described above, by the following technical solutions:
A kind of phytochelatin microbiobacterial agent, comprises following feed composition: plant active peptides, bacillus natto bacterium liquid, Pichia yeast liquid, lactobacterium casei bacterium liquid, Caulis Erycibes extract, ulva lactuca powder, gallnut extract, ferrous sulfate, fulvic acid potassium, sequestrant.
Below the further improvement to technique scheme:
The weight proportion of the feed composition of described phytochelatin microbiobacterial agent is: plant active peptides 8.5 parts, bacillus natto bacterium liquid 10 parts, Pichia yeast liquid 8 parts, lactobacterium casei bacterium liquid 5 parts, Caulis Erycibes extract 7.2 parts, ulva lactuca powder 43 parts, gallnut extract 26 parts, 3.8 parts, ferrous sulfate, fulvic acid potassium 26 parts, sequestrant 21 parts.
Described bacillus natto bacterium liquid: bacillus natto content is 40 × 10
9cPU/ml;
Described Pichia yeast liquid: pichia spp content is 16 × 10
9cPU/ml;
Described lactobacterium casei bacterium liquid: lactobacillus casei content is 22 × 10
9cPU/ml.
Described sequestrant is the mixture that EDTA and calcium superphosphate form by the weight ratio of 3.5:1.
A kind of preparation method of phytochelatin microbiobacterial agent, described preparation method comprises and prepares plant active peptides step: comprise pulverizing alkali and carry, described pulverizing alkali is carried: join in aqueous sodium hydroxide solution by soybean, Tuber Fleeceflower Root mixed powder, lixiviate 45min, the concentration of described aqueous sodium hydroxide solution is 1.5mol/L.
Described plant active peptides step of preparing also comprises acid spin-on and sinks: after vat liquor is centrifugal, with the hydrochloric acid soln of mass concentration 2%, adjust pH to 4.6, leave standstill 40min, then through the centrifugal 8min of rotating speed of 4000r/min.
Described plant active peptides step of preparing also comprises washing making beating and dries: throw out through twice warm water washings of 48 DEG C, then through the centrifugal 10min of rotating speed of 3400r/min; Albumen precipitation is smashed to pieces, breaks into homogenate; With sodium hydroxide solution adjust pH to 6.8-7.0; Dried by the slurries obtained, control inlet temperature is 148-158 DEG C, and hydrofuge temperature is 95-98 DEG C.
Described preparation method also comprises and prepares Caulis Erycibes extract: being dried by Stem of Obtuseleaf Erycibe to moisture content is that 7%, 200rpm pulverizes 10min, adds the water of Stem of Obtuseleaf Erycibe fine powder 3 times of volumes, leave standstill 18h, filter, macroporous resin column in filtrate, then the ethanolic soln wash-out resin column using 70% mass concentration.
Described preparation method also comprises chelating: the ethanol adding mass concentration 70%, reaction 40min; Described preparation method also comprises spray bacterium: during spray bacterium, stirring velocity is 60rpm.
Effective bacterial content is 4.8-5.2 × 109/g, and micronutrient levels is 42-45mg/g, and miscellaneous bacteria rate is 4.5-5.2%.
the present invention compared with prior art, has following beneficial effect:
1, the effective bacterial content of microbiobacterial agent of the present invention is high, rich trace elements, and miscellaneous bacteria rate is low; Effective bacterial content is 4.8-5.2 × 10
9individual/g, micronutrient levels is 42-45mg/g, and miscellaneous bacteria rate is 4.5-5.2%;
2, the resistance to storage of microbiobacterial agent of the present invention, microorganism active good stability; Store after 980 days, effective bacterial content is 5.2-5.8 × 10
9individual/g, miscellaneous bacteria rate is 4.7-5.7%;
3, adopt phytochelatin microbiobacterial agent of the present invention plantation peanut, disease and pest incidence reduces 58-60%, and peanut yield improves 7.6-8.5%, and the protein content of peanut improves 5.8-6.5%, and vitamin A content improves 20-22%, and Zn content improves 29-35%;
4, adopt phytochelatin microbiobacterial agent of the present invention plantation peanut after 1 year, obviously improve the microbial environment of growing area soil, probiotics content increases 3-3.5 doubly, and water capacity improves 40-48%, and plantation peanut 3 years, soil compaction phenomenon does not occur continuously.
Embodiment
Bacillus natto of the present invention (
bacillusnatto) preserving number is CICC20637; Pichia spp (
pichiasp.) preserving number is ACCC21000; Lactobacterium casei (
lactobacilluscasei) preserving number is ACCC10639; Above-mentioned bacterial classification is all from commercially available.
embodiment 1 one kinds of phytochelatin microbiobacterial agents
The raw material of described microbiobacterial agent comprises the component of following weight proportion:
Plant active peptides 7.9 parts, bacillus natto bacterium liquid 8 parts, Pichia yeast liquid 6 parts, lactobacterium casei bacterium liquid 3 parts, Caulis Erycibes extract 5.5 parts, ulva lactuca powder 41 parts, gallnut extract 24 parts, 3 parts, ferrous sulfate, fulvic acid potassium 20 parts, sequestrant 19 parts;
Described bacillus natto bacterium liquid: bacillus natto content is 40 × 10
9cPU/ml;
Described Pichia yeast liquid: pichia spp content is 16 × 10
9cPU/ml;
Described lactobacterium casei bacterium liquid: lactobacillus casei content is 22 × 10
9cPU/ml;
Described sequestrant is the mixture that EDTA and calcium superphosphate form by the weight ratio of 3.5:1.
embodiment 2 one kinds of phytochelatin microbiobacterial agents
The raw material of described microbiobacterial agent comprises the component of following weight proportion:
Plant active peptides 8.5 parts, bacillus natto bacterium liquid 10 parts, Pichia yeast liquid 8 parts, lactobacterium casei bacterium liquid 5 parts, Caulis Erycibes extract 7.2 parts, ulva lactuca powder 43 parts, gallnut extract 26 parts, 3.8 parts, ferrous sulfate, fulvic acid potassium 26 parts, sequestrant 21 parts;
Described bacillus natto bacterium liquid: bacillus natto content is 40 × 10
9cPU/ml;
Described Pichia yeast liquid: pichia spp content is 16 × 10
9cPU/ml;
Described lactobacterium casei bacterium liquid: lactobacillus casei content is 22 × 10
9cPU/ml;
Described sequestrant is the mixture that EDTA and calcium superphosphate form by the weight ratio of 3.5:1.
embodiment 3 one kinds of phytochelatin microbiobacterial agents
The raw material of described microbiobacterial agent comprises the component of following weight proportion:
Plant active peptides 8.9 parts, bacillus natto bacterium liquid 12 parts, Pichia yeast liquid 10 parts, lactobacterium casei bacterium liquid 7 parts, Caulis Erycibes extract 8.5 parts, ulva lactuca powder 45 parts, gallnut extract 29 parts, 4.5 parts, ferrous sulfate, fulvic acid potassium 30 parts, sequestrant 23 parts;
Described bacillus natto bacterium liquid: bacillus natto content is 40 × 10
9cPU/ml;
Described Pichia yeast liquid: pichia spp content is 16 × 10
9cPU/ml;
Described lactobacterium casei bacterium liquid: lactobacillus casei content is 22 × 10
9cPU/ml;
Described sequestrant is the mixture that EDTA and calcium superphosphate form by the weight ratio of 3.5:1.
the preparation method of embodiment 4 one kinds of phytochelatin microbiobacterial agents
Step 1, prepare plant active peptides
A. pulverize alkali to carry
Be crushed to 200 orders by cleaning the soybean, the Tuber Fleeceflower Root that dry, described soybean and the mass ratio of Tuber Fleeceflower Root are 1:3; Soybean, Tuber Fleeceflower Root mixed powder are joined in aqueous sodium hydroxide solution, lixiviate 45min, through gauze coarse filtration, obtain vat liquor; The concentration of described aqueous sodium hydroxide solution is 1.5mol/L.
B. acid spin-on is sunk
The vat liquor that above-mentioned steps obtains, through the centrifugal 8min of rotating speed of 4000r/min, gets supernatant liquor;
In supernatant liquor, add the hydrochloric acid soln of mass concentration 2%, adjust pH to 4.6, leave standstill 40min;
Again through the centrifugal 8min of rotating speed of 4000r/min, taking precipitate.
C. making beating is washed
The throw out that above-mentioned steps obtains is through twice warm water washings of 48 DEG C, then through the centrifugal 10min of rotating speed of 3400r/min, abandoning supernatant, obtains albumen precipitation; Albumen precipitation tissue mashing machine is smashed to pieces, adds the water with albumen precipitation same volume, break into homogenate; Use sodium hydroxide solution adjust pH to 6.8-7.0 again.
D. dry
Slurries above-mentioned steps obtained carry out spray dries, and control inlet temperature is 148-158 DEG C, and hydrofuge temperature is 95-98 DEG C; Get product after oven dry plant active peptides.
Step 2, preparation bacterium liquid
Respectively bacillus natto, pichia spp, lactobacterium casei carried out the activation of bacterial classification, spread cultivation, ferment, obtained bacillus natto bacterium liquid, Pichia yeast liquid, lactobacterium casei bacterium liquid;
Described bacillus natto bacterium liquid: bacillus natto content is 40 × 10
9cPU/ml;
Described Pichia yeast liquid: pichia spp content is 16 × 10
9cPU/ml;
Described lactobacterium casei bacterium liquid: lactobacillus casei content is 22 × 10
9cPU/ml.
Step 3, prepare Caulis Erycibes extract
Being dried by Stem of Obtuseleaf Erycibe to moisture content is that 7%, 200rpm pulverizes 10min, obtains Stem of Obtuseleaf Erycibe fine powder;
Add the water of Stem of Obtuseleaf Erycibe fine powder 3 times of volumes, leave standstill 18h, through filtering, removing filter residue, retains filtrate;
By macroporous resin column in filtrate, be washed till effluent liquid with distilled water colourless;
Use the ethanolic soln wash-out resin column of 70% mass concentration again, collect elutriant;
Adopt spray-drying process that elutriant is made dry powder, obtain Caulis Erycibes extract.
Step 4, weighing
Each raw material is taken respectively by composition of raw materials, for subsequent use.
Step 5, chelating
Sequestrant is added in reactor, open and stir, spray into plant active peptides, add the ethanol of mass concentration 70%, after reaction 40min, through suction filtration, washing, drying step, obtain chelating peptide.
Step 6, spray bacterium
Ulva lactuca powder, gallnut extract, ferrous sulfate, fulvic acid potassium and the chelating peptide prepared, Caulis Erycibes extract are mixed, join in reactor, after stirring 20min with the speed of 120rpm, stirring velocity is reduced to 60rpm, limit is stirred, while spray into bacterium liquid; During spray bacterium, spray into bacillus natto bacterium liquid, Pichia yeast liquid and lactobacterium casei bacterium liquid successively;
After spray bacterium completes, leave standstill 12h, blowing, obtained finished product microbiobacterial agent.
result detects:
(1) carry out Indexs measure to microbiobacterial agent prepared by the present invention, detected result is in table 1;
Microbiobacterial agent Indexs measure result prepared by table 1 the present invention
From table 1, the effective bacterial content of microbiobacterial agent of the present invention is 4.8-5.2 × 10
9individual/g, miscellaneous bacteria rate is 4.5-5.2%, and moisture content is 22-26%, and micronutrient levels is 42-45mg/g.
(2) microbiobacterial agent of the present invention is after storing 980 days, carries out microorganism detection, the results are shown in Table 2;
Table 2 microbiobacterial agent stores the detected result after 980 days
From table 2, the resistance to storage of microbiobacterial agent of the present invention; After storing 980 days, effective bacterial content is 5.2-5.8 × 10
9individual/g, miscellaneous bacteria rate is 4.7-5.7%.
(3) adopt phytochelatin microbiobacterial agent of the present invention plantation peanut, replace common fertilizer, compared with routine techniques, when rate of fertilizer application is identical, obviously reduce disease and pest incidence, improve peanut yield and quality; Specific targets are in table 3;
Table 3 adopts phytochelatin microbiobacterial agent of the present invention to plant the index of peanut
From table 3, adopt phytochelatin microbiobacterial agent of the present invention to plant peanut, disease and pest incidence reduces 58-60%, peanut yield improves 7.6-8.5%, the protein content of peanut improves 5.8-6.5%, and vitamin A content improves 20-22%, and Zn content improves 29-35%.
(4) through test, use phytochelatin microbiobacterial agent of the present invention plantation peanut after 1 year, obviously improve the microbial environment of growing area soil, probiotics content increases 3-3.5 doubly, water capacity improves 40-48%, and plantation peanut 3 years, soil compaction phenomenon does not occur continuously.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a phytochelatin microbiobacterial agent, is characterized in that: comprise following feed composition: plant active peptides, bacillus natto bacterium liquid, Pichia yeast liquid, lactobacterium casei bacterium liquid, Caulis Erycibes extract, ulva lactuca powder, gallnut extract, ferrous sulfate, fulvic acid potassium, sequestrant.
2. a kind of phytochelatin microbiobacterial agent according to claim 1, is characterized in that: the weight proportion of the feed composition of described phytochelatin microbiobacterial agent is: plant active peptides 8.5 parts, bacillus natto bacterium liquid 10 parts, Pichia yeast liquid 8 parts, lactobacterium casei bacterium liquid 5 parts, Caulis Erycibes extract 7.2 parts, ulva lactuca powder 43 parts, gallnut extract 26 parts, 3.8 parts, ferrous sulfate, fulvic acid potassium 26 parts, sequestrant 21 parts.
3. a kind of phytochelatin microbiobacterial agent according to claim 1, is characterized in that: described bacillus natto bacterium liquid: bacillus natto content is 40 × 10
9cPU/ml;
Described Pichia yeast liquid: pichia spp content is 16 × 10
9cPU/ml;
Described lactobacterium casei bacterium liquid: lactobacillus casei content is 22 × 10
9cPU/ml.
4. a kind of phytochelatin microbiobacterial agent according to claim 1, is characterized in that: described sequestrant is the mixture that EDTA and calcium superphosphate form by the weight ratio of 3.5:1.
5. the preparation method of a phytochelatin microbiobacterial agent, it is characterized in that: described preparation method comprises and prepares plant active peptides step: comprise pulverizing alkali and carry, described pulverizing alkali is carried: join in aqueous sodium hydroxide solution by soybean, Tuber Fleeceflower Root mixed powder, lixiviate 45min, the concentration of described aqueous sodium hydroxide solution is 1.5mol/L.
6. the preparation method of a kind of phytochelatin microbiobacterial agent according to claim 5, it is characterized in that: described plant active peptides step of preparing also comprises acid spin-on and sinks: after vat liquor is centrifugal, with the hydrochloric acid soln of mass concentration 2%, adjust pH to 4.6, leave standstill 40min, then through the centrifugal 8min of rotating speed of 4000r/min.
7. the preparation method of a kind of phytochelatin microbiobacterial agent according to claim 5, it is characterized in that: described plant active peptides step of preparing also comprises washing making beating and dries: throw out through twice warm water washings of 48 DEG C, then through the centrifugal 10min of rotating speed of 3400r/min; Albumen precipitation is smashed to pieces, breaks into homogenate; With sodium hydroxide solution adjust pH to 6.8-7.0; Dried by the slurries obtained, control inlet temperature is 148-158 DEG C, and hydrofuge temperature is 95-98 DEG C.
8. the preparation method of a kind of phytochelatin microbiobacterial agent according to claim 5, it is characterized in that: described preparation method also comprises and prepares Caulis Erycibes extract: by Stem of Obtuseleaf Erycibe dry to moisture content be 7%, 200rpm pulverizes 10min, add the water of Stem of Obtuseleaf Erycibe fine powder 3 times of volumes, leave standstill 18h, filter, macroporous resin column in filtrate, then the ethanolic soln wash-out resin column using 70% mass concentration.
9. the preparation method of a kind of phytochelatin microbiobacterial agent according to claim 5, is characterized in that: described preparation method also comprises chelating: the ethanol adding mass concentration 70%, reaction 40min; Described preparation method also comprises spray bacterium: during spray bacterium, stirring velocity is 60rpm.
10. a kind of phytochelatin microbiobacterial agent according to claim 1, is characterized in that: effectively bacterial content is 4.8-5.2 × 10
9individual/g, micronutrient levels is 42-45mg/g, and miscellaneous bacteria rate is 4.5-5.2%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510568818.8A CN105061093B (en) | 2015-09-09 | 2015-09-09 | A kind of phytochelatin microbial bacterial agent and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510568818.8A CN105061093B (en) | 2015-09-09 | 2015-09-09 | A kind of phytochelatin microbial bacterial agent and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105061093A true CN105061093A (en) | 2015-11-18 |
| CN105061093B CN105061093B (en) | 2018-01-23 |
Family
ID=54490669
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510568818.8A Active CN105061093B (en) | 2015-09-09 | 2015-09-09 | A kind of phytochelatin microbial bacterial agent and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105061093B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108314576A (en) * | 2018-02-11 | 2018-07-24 | 荔浦县万家兴果蔬专业合作社 | A kind of diseases prevention base manure of shatian pomelo and preparation method thereof |
| CN113995142A (en) * | 2021-11-08 | 2022-02-01 | 英国纽歌芬生物科技国际集团控股有限公司 | Composition containing phytochelatin and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1840681A (en) * | 2006-02-08 | 2006-10-04 | 沈阳农业大学 | Process for producing functional soybean peptide by two-step method |
| CN101671212A (en) * | 2009-09-28 | 2010-03-17 | 上海创博现代自然农业（集团）有限公司 | Microbial agent capable of reducing leaf vegetable plant diseases and insect pests and preparing method thereof |
| KR100998788B1 (en) * | 2008-08-07 | 2010-12-06 | 주식회사 코스템 | Aquous organic fertilizer from stevia |
| CN103553828A (en) * | 2013-11-12 | 2014-02-05 | 天津北洋百川生物技术有限公司 | Liquid compound biological fertilizer with long shelf life as well as preparation method and application thereof |
| CN103772011A (en) * | 2013-12-27 | 2014-05-07 | 天津北洋百川生物技术有限公司 | Special compound biological liquid-state bacterial fertilizer for grain |
-
2015
- 2015-09-09 CN CN201510568818.8A patent/CN105061093B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1840681A (en) * | 2006-02-08 | 2006-10-04 | 沈阳农业大学 | Process for producing functional soybean peptide by two-step method |
| KR100998788B1 (en) * | 2008-08-07 | 2010-12-06 | 주식회사 코스템 | Aquous organic fertilizer from stevia |
| CN101671212A (en) * | 2009-09-28 | 2010-03-17 | 上海创博现代自然农业（集团）有限公司 | Microbial agent capable of reducing leaf vegetable plant diseases and insect pests and preparing method thereof |
| CN103553828A (en) * | 2013-11-12 | 2014-02-05 | 天津北洋百川生物技术有限公司 | Liquid compound biological fertilizer with long shelf life as well as preparation method and application thereof |
| CN103772011A (en) * | 2013-12-27 | 2014-05-07 | 天津北洋百川生物技术有限公司 | Special compound biological liquid-state bacterial fertilizer for grain |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108314576A (en) * | 2018-02-11 | 2018-07-24 | 荔浦县万家兴果蔬专业合作社 | A kind of diseases prevention base manure of shatian pomelo and preparation method thereof |
| CN113995142A (en) * | 2021-11-08 | 2022-02-01 | 英国纽歌芬生物科技国际集团控股有限公司 | Composition containing phytochelatin and preparation method thereof |
| CN113995142B (en) * | 2021-11-08 | 2022-04-26 | 英国纽歌芬生物科技国际集团控股有限公司 | Composition containing phytochelatin and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105061093B (en) | 2018-01-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102180743B (en) | Method for producing insect resistance organic compound fertilizer by fermentation of agricultural organic wastes | |
| CN104744171A (en) | Novel green biological organic fertilizer and preparation method thereof | |
| CN102180737B (en) | Method for producing organic compound fertilizer by fermenting agricultural organic waste | |
| CN105036851A (en) | Special liquid water flush fertilizer for potatoes and preparation method for special liquid water flush fertilizer for potatoes | |
| CN105218177A (en) | Utilize silkworm excrement fermentation for the method for composite microbic bacterial fertilizer | |
| CN103086771A (en) | Bioorganic fertilizer preparation method, bioorganic fertilizer and application of bioorganic fertilizer | |
| CN107162835A (en) | One plant nutrient liquor | |
| CN105367222A (en) | Biological organic fertilizer and preparation method thereof | |
| CN103360132A (en) | Bio-organic fertilizer with kitchen garbage as raw material and preparation method thereof | |
| CN106986696A (en) | Utilize soil conditioner obtained by biological fermentation bed waste material and preparation method thereof | |
| CN103664389A (en) | Method for preparing special bio-organic fertilizer for tea by using plant extract residues | |
| CN110256148A (en) | A kind of diseases prevention promoting root growth microbial-bacterial fertilizer and preparation method thereof suitable for water-fertilizer integral system | |
| CN102180716B (en) | Method for producing organic compound fertilizer by fermenting animal manure | |
| CN109824461A (en) | Compound microbial fertilizer | |
| CN105084955A (en) | Method for preparing organic fertilizer from cow dung and sheep manure | |
| CN106591147A (en) | Aspergillus niger NJDL-12 bacterial strain and application thereof to improvement of coastal saline-alkali soil | |
| CN110272315A (en) | Environment-friendly chemical fertilizer capable of improving saline-alkali soil and preparation method thereof | |
| CN106348795A (en) | Astragalus smicus/straw organic fertilizer and preparation method thereof | |
| CN103524220A (en) | Production method for rice seedling cultivation dedicated organic fertilizer | |
| CN100471824C (en) | Pricklyash seed acid organic granule fertilizer and its processing method | |
| CN106083481B (en) | A kind of production technology improveing salt-soda soil bio-organic fertilizer | |
| CN105061093A (en) | Phytochelatin microbial agent and preparation method thereof | |
| CN105272557A (en) | Fertilizer synergist for inhibiting soil borne diseases | |
| CN105272550A (en) | Lodging-resistant wheat fertilizer synergist | |
| CN105272572A (en) | Cultivation base fertilizer for juvenile stage of pear trees and preparation method of base fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 262500 1000 meters east of entrance 10, Weifang section, Jiqing Road, Qingzhou City, Weifang City, Shandong Province Patentee after: Shandong Xiangyue Bioengineering Technology Co.,Ltd. Address before: 262500, 100m east of entrance 10, Weifang section, Jiqing Road, Qingzhou City, Weifang City, Shandong Province Patentee before: SHANDONG XIANGYUE BIO ENGINEERING TECHNOLOGY Co.,Ltd. |
|
| CP03 | Change of name, title or address | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220525 Address after: 262700 No. 063, Guhuai Road, Shengcheng street, Shouguang City, Weifang City, Shandong Province Patentee after: Shandong zhongqingnong Biotechnology Co.,Ltd. Address before: 262500 1000 meters east of entrance 10, Weifang section, Jiqing Road, Qingzhou City, Weifang City, Shandong Province Patentee before: Shandong Xiangyue Bioengineering Technology Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220801 Address after: 262737 north of Zhujiang West Street and west of Beihai branch road, advanced manufacturing industrial park, Binhai District, Weifang City, Shandong Province Patentee after: China bacteria Holding Group Co.,Ltd. Address before: 262700 No. 063, Guhuai Road, Shengcheng street, Shouguang City, Weifang City, Shandong Province Patentee before: Shandong zhongqingnong Biotechnology Co.,Ltd. |
|
| TR01 | Transfer of patent right |