CN103086771A - Bioorganic fertilizer preparation method, bioorganic fertilizer and application of bioorganic fertilizer - Google Patents
Bioorganic fertilizer preparation method, bioorganic fertilizer and application of bioorganic fertilizer Download PDFInfo
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Abstract
The invention relates to the technical field of biological fertilizers, and discloses a bioorganic fertilizer preparation method, a bioorganic fertilizer and an application of the bioorganic fertilizer. The bioorganic fertilizer is prepared by mixing high-fiber organic matters comprising rice bran, soybean meal, corn flour, peanut bran and the like and beneficial microbes according to a reasonable ratio through a technology. The prepared bioorganic fertilizer has a pitch-black and bright appearance, has a soy fragrance, and has effective living bacteria count of 0.05 billion cuf/g or more, an organic matter content of 47% or more, a water content of 25% or less, a Fecal coliform count of 0.11/g or less, and a roundworm mortality of 95%, and all the indexes are better than Chinese standards. The preparation method has the advantages of no use of animal waste, clean raw material source, and clean and odorless production workshop, creates a good work environment for production works, and accords with the environmental protection demand.
Description
Technical field
The present invention relates to the bio-feritlizer technical field, more specifically, relate to a kind of preparation method of biological organic fertilizer and the biological organic fertilizer for preparing and application.
Background technology
Biological organic fertilizer refer to microorganisms with specific functions with mainly take plant and animal residues (as feces of livestock and poultry, agricultural crop straw etc.) for originating and having the fertilizer of microbial fertilizer and fertilizer effect through the class that harmless treatment, the organic materials that becomes thoroughly decomposed are composited concurrently.The biological organic fertilizer nutritive element is complete, can improve soil, improves the soil compaction that uses chemical fertilizer to cause.Improve soil physical and chemical property, strengthen the ability of soil water-retaining, fertilizer conservation, fertilizer.Beneficial microorganism in biological organic fertilizer forms mutual symbiosis Relationship With Proliferation with Soil Microorganism after entering soil, suppressing harmful bacteria grows and is converted into probiotics, interact, mutually promote, play the synergy of colony, probiotics produces a large amount of meta-bolitess in the growth and breeding process, impel organic decomposition and inversion, can directly or indirectly for crop provides multiple nutrients and pungent, promote and the regulation and control plant growth.Improve soil porosity, penetrating exchangeability and plant survival rate, increase probiotics and soil microorganisms and population.Simultaneously, the advantage probiotics group energy that forms at crop root suppresses harmful pathogenic bacteria procreation, strengthens the degeneration-resistant resistance against diseases of crop and reduces the disease index of continuous cropping crop, uses year after year and can greatly alleviate continuous cropping obstacle.Environmental contamination reduction to people, animal, environmental safety, nontoxic, is a kind of environment-friendly type fertilizer.
But at present, biological organic fertilizer of the prior art has all used animal excrement in order to reach certain fertilizer efficiency, has used the poultry manures such as chicken manure, sheep excrement, pig manure as the bio-feritlizer in patent CN102633539, this causes production plant to produce unpleasant stink, and production environment is abominable.
Biological fermentation is subject to many factors, and the raw material of different ingredients and the proportioning resulting obvious technical effects of fermenting is different, and the physico-chemical property of fertilizer and fertilizer efficiency are also significantly different.Only in reasonably formula and proportioning and under in conjunction with simple and effective preparation method, could finally obtain environmental protection, green, biological organic fertilizer that fertilizer efficiency is good.
Summary of the invention
The object of the invention is to overcome the technical deficiency of existing biological organic fertilizer, a kind of preparation method of biological organic fertilizer is provided, adopts the inventive method, successfully prepare cleaning, fertilizer efficiency is good and has the bio-organic fertilizer of soy sauce fragrance, simple to operate, environment is good.
Another object of the present invention is to provide the fertilizer that described preparation method prepares.
A further object of the invention is to provide the application of described biological organic fertilizer.
Purpose of the present invention is achieved by the following technical programs:
A kind of preparation method of biological organic fertilizer is provided, comprises the following steps:
S1. rice bran, dregs of beans, peanut press pulp and bean dregs are mixed in proportion to get mixture, with the mixture tiling, the mixture with after inosine mother liquor nutritive medium immersion tiling adds yeast, in 50～60 ℃ of bottom fermentations, carries out turning in fermenting process and processes;
After the described fermentation of S2.S1 is completed, the temperature of mixture is down to 26～30 ℃, adds microbial nutrient solution, the microorganism in microbial nutrient solution bred in mixture 10～20 days, namely got described biological organic fertilizer.
The applicant is devoted to high fiber organism is prepared the high-quality biological fertilizer, with stench, pollution and the secondary pollution of avoiding using ight soil and municipal sludge to be caused based on years of researches analysis and summary always.But long-term a large amount of experimental summary is found, if the organic selection compatibility of high fiber is unreasonable, can not be for the multiple beneficial microorganism provide mutual utilization and the mutual good environment of symbiosis, also just be difficult to really to realize simultaneously obtaining cleaning, fertilizer efficiency is good and has the biological organic fertilizer of soy sauce fragrance.
The present invention adopts rice bran, dregs of beans, peanut press pulp and bean dregs to mix in proportion and obtains basic mixture, dregs of beans is a kind of byproduct of soybean through obtaining after extracting bean oil, different according to the method for extracting, can be divided into and soak dregs of beans and two and soak two kinds of dregs of beans, wherein the byproduct after the extraction extracting bean oil soaks dregs of beans as one, and first get oil with squeezing, then get after oil the byproduct of gained through lixiviate and be called two and soak dregs of beans.Make the bean dregs of soya-bean milk generation and almost forgotten by people because mouthfeel is poor, usually as waste.But in bean dregs, a large amount of protein are along with the process of extracting soya-bean milk is extracted away, contain protein and a large amount of Mierocrystalline cellulose of small part in bean dregs, and dregs of beans is the product after extracting bean oil, has higher protein content, and is higher than bean dregs.The present invention will be both according to dregs of beans 5～15 weight parts, the ratio of bean dregs 5～15 weight parts matches, be equipped with again rice bran 60～80 weight parts, peanut press pulp 5～15 weight parts, the indexes such as carbon-nitrogen ratio in the fermented material have been improved, experimental results show that described compatibility and proportioning have played the effect of the fermentation that heats up better, provide good biological organic fertilizer fermenting mixture material matrix.
More preferably, the parts by weight of rice bran, dregs of beans, peanut press pulp and bean dregs are: 70 parts, rice bran, 10 parts of dregs of beans, 10 parts of peanut press pulps, 10 parts, bean dregs.
S1 soaks the mixture of tiling with inosine mother liquor nutritive medium as long as guarantee that the mixed material of inosine mother liquor nutritive medium liquid surrounds.Preferably, the consumption of the described inosine mother liquor of S1 nutritive medium is according to the gross weight of mixture: the volume of inosine mother liquor nutritive medium is that 10Kg:1L determines.
Preferably, in the described fermenting process of S1, add the inedible oil fat that accounts for mixture quality 1.5～2% after the 5th～7 day; The inedible oil fat that more preferably, can add mixture quality 1.5%.Described inedible oil fat preferably glycerine or edible waste oil (waste grease that for example produces in diet processes).
Preferably, it is after 5～7 days, mixture to be carried out turning for the first time in fermentation that the described turning of S1 is processed, later on every turning in 1～3 month once; The time of described fermentation is 5～6 months.Described overthrowing according to material quantity can use iron Chinese catalpa or navvy to overthrow.
More preferably, the described inedible oil fat that accounts for mixture (material that rice bran, dregs of beans, peanut press pulp, bean dregs and microbial nutrient solution mix) quality 1.5～2% that adds when fermentation was carried out turning for the first time to mixture after 5～7 days of S1.The inedible oil fat that preferably, can add mixture quality 1.5%; Described inedible oil fat preferably glycerine or edible waste oil.
Preferably, contain phosphate solubilizing bacteria, potassium solubilizing bacteria and vinelandii in the described microbial nutrient solution of S2.Contain in the described every milliliter of microbial nutrient solution of S2: phosphate solubilizing bacteria 0.1～0.3 hundred million cuf/mL, potassium solubilizing bacteria 0.1～0.3 hundred million cuf/mL and vinelandii 0.1～0.3 hundred million cuf/mL.The present invention uses described three kinds of probioticss, and rational compatibility and addition have accurately been determined, under step of preparation process provided by the invention and condition, mutually utilize with the formed System forming of described mixture, the mutual coenocorrelation of symbiosis, guaranteed that finally the biological organic fertilizer fertilizer efficiency for preparing is good, the production process odorless.
Most preferably, contain in the described every milliliter of microbial nutrient solution of S2: phosphate solubilizing bacteria 0.2 hundred million cuf/mL, potassium solubilizing bacteria 0.2 hundred million cuf/mL and vinelandii 0.2 hundred million cuf/mL.
Preferably, the preparation method of the described microbial nutrient solution of S2 is: phosphate solubilizing bacteria, potassium solubilizing bacteria and vinelandii are rendered in inosine mother liquor nutritive medium according to addition, and cellar culture obtains, and is preferable over 20～30 ℃ and cultivates 50～60 days and get final product.
In described microbial nutrient solution, described phosphate solubilizing bacteria be preferably bacillus megaterium (
Bacillus megaterium), described potassium solubilizing bacteria be preferably silicate bacteria (
Silicate bacteria), described vinelandii be preferably vinelandii (
Azotobacter sp), be all conventional the purchase.
Preferably, the consumption of the described microbial nutrient solution of S2 is according to the gross weight of mixture: the volume of microbial nutrient solution is that 10Kg:2L determines.
The invention provides the biological organic fertilizer that described preparation method prepares, simple, production environment cleaning odorless.Prepare biological organic fertilizer according to step of the present invention and technique, the technique effect that different batches obtains is highly stable.The equal outward appearance of the biological organic fertilizer for preparing is pitch-black glossy, has soy sauce fragrance, and living bacteria count is not less than 0.5 hundred million cuf/g, organic matter is not less than 47%, and moisture is not more than 25%, and the colibacillus of excrement number is not more than 0.11/g, the roundworm mortality ratio is not less than 95%, and all indexs all are better than national standard.
The present invention also provides the application of described biological organic fertilizer, is applicable to the agroforestry crop production, is specially adapted to the application aspect plantation lichee, cabbage heart, shatian pomelo or paddy rice.
The present invention has following beneficial effect:
the present invention is with rice bran, dregs of beans, Semen Maydis powder, peanut press pulp prepares biological organic fertilizer jointly as high fiber organism and beneficial microorganism, by microbial fermentation and organic physico-chemical property are creatively analyzed also in conjunction with long-term a large amount of experiment, found out suitable proportioning raw materials, success obtains biological organic fertilizer of the present invention, biological organic fertilizer has photosynthetic property, antiviral property, decompose soil nutrient, short living multiple ferment, the good microorganism biological characteristics such as fiber decomposability, fertilizer efficiency is good, distribute soy sauce fragrance, thoroughly changed the dirty of conventional fertilizers, the defective such as smelly, for applying of biological organic fertilizer provides strong technical support.
The bio-organic fertilizer that the present invention prepares does not use animal excrement, and raw material sources are clean, under suitable production method step operation, has guaranteed that production plant is clean, and odorless for the direct labor has created good Working environment, meets the environmental protection demand.
Further, the present invention to the control of critical process, has done creationary improving and optimizating to the processing step of preparation and proportioning raw materials and addition sequence, both the kill harmful microorganism in preparation process, guarantee the abundant fermentative action of beneficial microorganism, starting material are carried out slaking decompose; In biological organic fertilizer of the present invention, used the inedible oil fat of sufficient quantity, the fertilizer moistening thickness that can become, has the preserve moisture and fertility effect, the fertilizer efficiency that has guaranteed organic fertilizer of the present invention rises to 24 months, compare with the general bio-organic fertilizer fertilizer efficiency of 6 months, phenomenal growth, stability significantly improves.
After testing, biological organic fertilizer outward appearance of the present invention is pitch-black glossy, has soy sauce fragrance, living bacteria count is not less than 0.5 hundred million cuf/g, and organic matter is not less than 47%, and moisture is not more than 25%, the colibacillus of excrement number is not more than 0.11/g, and the roundworm mortality ratio is not less than 95%, and all indexs all are better than national standard.
Embodiment
Further explain the present invention below in conjunction with specific embodiment, unless stated otherwise, the raw material that the embodiment of the present invention adopts and method are the conventional raw material of the art and method.For convenience of description, the inosine mother liquor nutritive medium described in the embodiment of the present invention is the inosine liquid that directly adopts available from Xinghu Biotech Co., Ltd., Zhaoqing City, Guangdong Prov..Yeast described in the embodiment of the present invention is available from Guangdong Province microbiological analysis inspection center, and Article Number is AS2.180.The similar product that those skilled in the art of the present technique also can adopt other buyings to originate.Unless stated otherwise, the raw material that adopts in the embodiment of the present invention and equipment are raw material and the equipment in the conventional source of the art.
Embodiment 1
With bacillus megaterium (available from Guangdong Province microbiological analysis inspection center, Article Number is ACCC11011), silicate bacteria is (available from Agricultural University Of South China, Article Number is IM6), vinelandii are (available from Guangdong Province microbiological analysis inspection center, Article Number is AS1.222) render in inosine mother liquor nutritive medium and cultivate, make in every milliliter of microbial nutrient solution and contain: phosphate solubilizing bacteria 0.1 hundred million cuf/mL, potassium solubilizing bacteria 0.1 hundred million cuf/mL, vinelandii 0.1 hundred million cuf/mL.(do not need camera bellows) and cultivate the microbial nutrient solution that namely obtained containing various trace elements in 50～60 days under 25～30 ℃.Above-mentioned bacterial strains is not limited to described purchasing channel, also can be self-control or other sources.Inosine mother liquor nutritive medium mainly contains organic matter, nitrogen, phosphorus, potassium, humic acid and glucose.
Preparation biological organic fertilizer step is as follows:
S1. with 60 parts, rice bran, 5 parts of dregs of beans, get mixture after 5 parts of raw material blendings such as grade of 5 parts of peanut press pulps and bean dregs, mixture is tiled, the thickness of preferred tiling is 10cm, press the mixture (guaranteeing that the mixed material of liquid surrounds) that 1Kg:0.1L inosine mother liquor nutritive medium soaks tiling, add yeast (available from Guangdong Province microbiological analysis inspection center in mixture, Article Number is AS2.180, add-on is 6L, 8,000,000,000 cuf/L) be maintained at 55～60 ℃ of fermentations, ferment and after 5～7 days, starting material are carried out turning, later on every turning in 1～3 month 1 time, continuing fermentation 5～6 months, the mixture slaking is decomposed,
The temperature of the mixture that after the described fermentation of S2.S1 is completed, slaking is decomposed is down to 26～30 ℃, gross weight according to mixture: the volume of microbial nutrient solution is that 10Kg:2L adds microbial nutrient solution, microorganism in microbial nutrient solution bred 10～20 days in the mixture that slaking is decomposed, and namely got biological organic fertilizer of the present invention.Be powdery after the air-dry drying of fertilizer, outward appearance is pitch-black glossy, has soy sauce fragrance, machinery-free impurity.
Embodiment 2
With bacillus megaterium (available from Guangdong Province microbiological analysis inspection center, Article Number is ACCC11011), silicate bacteria is (available from Agricultural University Of South China, Article Number is IM6), vinelandii are (available from Guangdong Province microbiological analysis inspection center, Article Number is AS1.222) render in inosine mother liquor nutritive medium and cultivate, make in every milliliter of microbial nutrient solution and contain: phosphate solubilizing bacteria 0.2 hundred million cuf/mL, potassium solubilizing bacteria 0.2 hundred million cuf/mL, vinelandii 0.2 hundred million cuf/mL.(do not need camera bellows) and cultivate the microbial nutrient solution that namely obtained containing various trace elements in 50～60 days under 25～30 ℃.Above-mentioned bacterial strains is not limited to described purchasing channel, also can be self-control or other sources.Inosine mother liquor nutritive medium mainly contains organic matter, nitrogen, phosphorus, potassium, humic acid and glucose.
S1. with after the raw material blendings such as 15 parts, 80 parts, rice bran, 15 parts of dregs of beans, 15 parts of peanut press pulps and bean dregs mixture, with the mixture tiling, the thickness of preferred tiling is 12cm; Soak the mixture of tiling by 1Kg:0.1L inosine mother liquor nutritive medium (being purchased from Xinghu Biotech Co., Ltd., Zhaoqing City, Guangdong Prov.), add yeast (available from Guangdong Province microbiological analysis inspection center, Article Number is AS2.180, add-on is 6L, the same), be maintained at 55～60 ℃ of fermentations, ferment and after 5～7 days, starting material are carried out turning, later on every turning in 1～3 month once; Continuing fermentation 5～6 months, the mixture slaking is decomposed;
The temperature of the mixture that after the S2.S1 fermentation is completed, slaking is decomposed is down to 26～30 ℃, add the gross weight according to mixture: the volume of microbial nutrient solution is that 10Kg:2L adds microbial nutrient solution (preparation method is with embodiment 1), microorganism in microbial nutrient solution bred 10～20 days in the mixture that slaking is decomposed, namely get biological organic fertilizer of the present invention, be powdery after drying, outward appearance is pitch-black glossy, has soy sauce fragrance, machinery-free impurity.
Embodiment 3
With bacillus megaterium (available from Guangdong Province microbiological analysis inspection center, Article Number is ACCC11011), silicate bacteria is (available from Agricultural University Of South China, Article Number is IM6), vinelandii are (available from Guangdong Province microbiological analysis inspection center, Article Number is AS1.222) render in inosine mother liquor nutritive medium and cultivate, make in every milliliter of microbial nutrient solution and contain: phosphate solubilizing bacteria 0.3 hundred million cuf/mL, potassium solubilizing bacteria 0.3 hundred million cuf/mL, vinelandii 0.3 hundred million cuf/mL.(do not need camera bellows) and cultivate the microbial nutrient solution that namely obtained containing various trace elements in 50～60 days under 25～30 ℃.Above-mentioned bacterial strains is not limited to described purchasing channel, also can be self-control or other sources.Inosine mother liquor nutritive medium mainly contains organic matter, nitrogen, phosphorus, potassium, humic acid and glucose.
S1. with after the raw material blendings such as 10 parts, 70 parts, rice bran, 10 parts of dregs of beans, 10 parts of peanut press pulps and bean dregs mixture, with the mixture tiling, the thickness of preferred tiling is 15cm; Press the mixture that 1Kg:0.1L inosine mother liquor nutritive medium soaks tiling, add yeast (available from Guangdong Province microbiological analysis inspection center, Article Number is AS2.180, add-on is 7L), be maintained at 60～68 ℃ of fermentations, ferment and after 5～7 days, mixture is carried out turning, add the glycerine that accounts for mixture quality 1.5% in turning, the moistening thickness of material; Later on every turning in 1～3 month once, continuing fermentation 5～6 months, the mixture slaking is decomposed;
The temperature of the mixture that after the S2.S1 fermentation is completed, slaking is decomposed is down to 26～30 ℃, add the gross weight according to mixture: the volume of microbial nutrient solution is that 10Kg:2L adds microbial nutrient solution (preparation method is with embodiment 1), microorganism in microbial nutrient solution bred 10～20 days in the mixture that slaking is decomposed, namely get biological organic fertilizer of the present invention, be powdery after drying, outward appearance is pitch-black glossy, has soy sauce fragrance, machinery-free impurity.
Embodiment 4
S1. with after the raw material blendings such as 10 parts of 70 parts, rice bran, 10 parts of dregs of beans, 10 parts of peanut press pulps, 10 parts, bean dregs and Semen Maydis powder mixture, with the mixture tiling, the thickness of preferred tiling is 20cm; Press the mixture that 1Kg:0.1L inosine mother liquor nutritive medium soaks tiling, add yeast (available from Guangdong Province microbiological analysis inspection center, Article Number is AS2.180, and add-on is 7L), 60～68 ℃ of fermentations;
Ferment and after 5～7 days, mixture is carried out turning, every turning in 1～3 month once, add the inedible oil fat (selecting food waste oil, the remaining waste oil in each kitchen, hotel of originating) that accounts for mixture quality 2% in turning, the moistening thickness of material; Continuing fermentation 5～6 months, the mixture slaking is decomposed;
The temperature of the mixture that after the S2.S1 fermentation is completed, slaking is decomposed is down to 26～30 ℃, gross weight according to mixture: the volume of microbial nutrient solution is that 10Kg:2L adds microbial nutrient solution (preparation method is with embodiment 1), microorganism in microbial nutrient solution bred 10～20 days in the mixture that slaking is decomposed, and namely got biological organic fertilizer of the present invention.Be powdery after drying, outward appearance is pitch-black glossy, has soy sauce fragrance, machinery-free impurity.
The bio-organic fertilizer that embodiment 1～4 is made detects through Guangdong Province microbiological analysis inspection center and fertilizer quality supervision and inspection center of the Ministry of Agriculture, and the result of gained all is better than national standard, is shown in Table 1.
Table 1 bio-organic fertilizer detected result of the present invention
Wherein the validity period of embodiment 3 and embodiment 4 reaches 30 months.
5 kinds of application experiments of planting cabbage heart of embodiment
1. test site: test is carried out in the Da Dong of Fengcheng neighbourhood committee food market, Shaoguan City of Guangdong Province Xinfeng County, and experimental field soil property is silty loam, middle fertility, and preceding crop is fresh kidney beans.
2. test kind: slow cabbage heart.
3. for examination fertilizer: biological organic fertilizer of the present invention, living bacteria count are not less than 0.3 hundred million cuf/g, and organic matter is not less than 47%, N+P
2O
5+ K
2O＞=4%.
4. 4 processing are established in test, and each is processed 3 times and repeats, random alignment, residential quarter area 20m
2Cabbage heart is in sowing on December 30, and transplant January 30 next year, and mu is planted 2.5 ten thousand strains, and March 22, results were complete.Each Treatment Design is as follows:
Process one, before cabbage heart is transplanted, mu is executed 80kg biological organic fertilizer of the present invention (biological organic fertilizer of selecting the embodiment of the present invention 1 to prepare is used) and made base manure, topdresses: each drenched and executed biological organic fertilizer 10kg/ mu February 6 and February 19; Transplant rear 10 days every mu of points and execute three element compound fertilizer (15-15-15) 15kg, transplant rear 25 days every mu of points and execute three element compound fertilizer (15-15-15) 25kg.
Process two, before cabbage heart is transplanted, mu is executed the biological organic fertilizer of 80kg deactivation (deactivation of Autoclave inner high voltage); Topdress: February 6 and February 19, each drenched the biological organic fertilizer 10kg/ mu of executing deactivation; Transplant rear 10 days every mu of points and execute three element compound fertilizer (15-15-15) 15kg, transplant rear 25 days every mu of points and execute three element compound fertilizer (15-15-15) 25kg.
Process three, the front mu of cabbage heart transplanting executes well-rotted farmyard manure 500kg and three element compound fertilizer (15-15-15) 25kg makees base manure, spreads fertilizer over the fields after mixing, and the situation of topdressing is; Topdress: drench on February 6 and February 19 and execute urea 10kg/ mu, it is 1:100 that concentration is executed in pouring for the first time, and it is 1:50 that concentration is executed in pouring for the second time; Transplant rear 10 days every mu of points and execute three element compound fertilizer (15-15-15) 15kg, transplant rear 25 days every mu of points and execute three element compound fertilizer (15-15-15) 25kg.
Process four, blank (the full phase is not executed any fertilizer).
5. test-results:
(1) use biological organic fertilizer of the present invention to the impact of Growth of Brassica Parachinensis
Observe in process of the test, use the processing one of biological organic fertilizer of the present invention, cabbage heart leaf look dark green, and growing way is good, and blade is wider and thick, tender flower stalk is thicker, as can be seen from Table 2, the plant height increasing degree of processing a cabbage heart is 6.2%, 88.4% and 73.1%, and the width of blade increasing degree is respectively 13.3%, 76.5% and 103.3%, the blade length increasing degree is respectively 11.5%, 89.7% and 56.4%, and the thick increasing degree of stem is respectively 0.0%, 66.7% and 80.0%; The plant height increasing degree of processing two cabbage hearts is 3.3%, 77.4% and 68.2%, the width of blade increasing degree is respectively 7.1%, 66.7% and 96.0%, the blade length increasing degree is respectively 8.3%, 84.6% and 54.2%, and the thick increasing degree of stem is respectively 0.0%, 50.0% and 66.7%; The plant height increasing degree of processing three cabbage hearts is 6.1%, 87.1% and 72.5%, the width of blade increasing degree is respectively 12.5%, 77.8% and 96.9%, the blade length increasing degree is respectively 12.0%, 89.3% and 56.6%, and the thick increasing degree of stem is respectively 0.0%, 66.7% and 60.0%; The plant height increasing degree of processing four cabbage hearts is 3.6%, 70.6% and 66.7%, the width of blade increasing degree is respectively 7.7%, 64.3% and 91.3%, the blade length increasing degree is respectively 4.6%, 78.3% and 50.5%, and the thick increasing degree of stem is respectively 0.0%, 50.0% and 33.3%.During results, process a cabbage heart plant height, width of blade, length and stem rough segmentation not than the increase by 21.6%, 24.5%, 16.2% and 80.0% of processing two, plant height and width of blade are respectively than the minimizing 0.4%, 3.1% of processing three, and blade length and stem rough segmentation be not than the increase by 3.6%, 12.5% of processing three; Respectively than the increase by 36.4%, 38.6%, 36.5% and 125.0% of processing four.Presentation of results is compared with the biological organic fertilizer of using deactivation and the blank that do not apply fertilizer, uses biological organic fertilizer of the present invention and can promote Growth of Brassica Parachinensis, increases cabbage heart plant height, width of blade, length and stem thick.
Table 2 is used biological organic fertilizer of the present invention to the impact (unit: cm) of Growth of Brassica Parachinensis
(2) use biological organic fertilizer of the present invention to the impact of yield of flowering Chinese cabbage
As can be seen from Table 3, between processing one and processing two, volume variance is remarkable, and between processing three, yield of flowering Chinese cabbage difference is not remarkable, and between processing four, the poor heteropole is remarkable.Process an output the highest, process three and take second place, the 3rd of processing two is processed four output minimum.
The processing one residential quarter mean yield that cabbage heart is used biological organic fertilizer of the present invention is 28.7kg, folding per mu yield 956.7kg, and than processing two mu of volume increase 50.0kg, amplification is 5.5%, increase yield significantly; Than processing three mu of volume increase 6.7kg, amplification is 0.7%; Than processing four mu of volume increase 190.0kg, amplification is 24.8%, increases production extremely remarkable.Presentation of results is compared with processing two, three, four, and it is obvious that cabbage heart is used biological organic fertilizer production-increasing function of the present invention, and with conventional processing three contrasts of habits fertilization, both output approaches and slightly volume increase.
Table 3 is used biological organic fertilizer of the present invention to the impact of yield of flowering Chinese cabbage
The different lowercases in data back in table are test significant difference through LSR, and different capitalizations are extremely remarkable through LSR test difference.
Conclusion (of pressure testing): compare with the blank that does not apply fertilizer with the biological organic fertilizer of using deactivation, use biological organic fertilizer of the present invention and can promote Growth of Brassica Parachinensis, increase cabbage heart plant height, width of blade, length and stem thick, compared with the control, use biological organic fertilizer of the present invention and can promote the cabbage heart volume increase.
6 kinds of application experiments of planting cabbage heart of embodiment
1. test site: test is carried out in material pool village, river and mountain town, Zhaoqing City of Guangdong Province Fengkai County Red Star peasant household vegetable plot, experimental field in fertility under, soil property silty loam, preceding crop are Lettuce.
2. test kind: blue or green No. 6 cabbage hearts of oil.
3. for examination fertilizer: biological organic fertilizer of the present invention, living bacteria count are not less than 0.3 hundred million cuf/g, and organic matter is not less than 47%, N+P
2O
5+ K
2O＞=4%.
4. 4 processing are established in test, and each is processed 3 times and repeats, random alignment, residential quarter area 20m
2Cabbage heart is in sowing on February 10, and March 2 transplanted, and mu is planted 2.5 ten thousand strains, and April 6 gathered in the crops, and April 8, results were complete.Each Treatment Design is as follows:
Process one, before cabbage heart is transplanted, (March 2) mu is executed 80kg biological organic fertilizer of the present invention (biological organic fertilizer of selecting the embodiment of the present invention 2 to prepare is used) and made base manure, topdresses: each drenched and executed biological organic fertilizer 10kg/ mu March 9 and March 22; Transplant rear 10 days every mu of points and execute domestic composite fertilizer (15-15-15) 15kg, transplant rear 25 days every mu of points and execute domestic composite fertilizer (15-15-15) 25kg.
Process two, before cabbage heart is transplanted, (March 2) mu is executed the biological organic fertilizer of 80kg deactivation (deactivation of Autoclave inner high voltage); Topdress: March 9 and March 22, each drenched the biological organic fertilizer 10kg/ mu of executing deactivation; Transplant rear 10 days every mu of points and execute domestic composite fertilizer (15-15-15) 15kg, transplant rear 25 days every mu of points and execute domestic composite fertilizer (15-15-15) 25kg.
Process three, cabbage heart transplanting front (March 2) mu executes well-rotted farmyard manure 500kg and domestic composite fertilizer (15-15-15) 50kg makees base manure, spreads fertilizer over the fields after mixing, and the situation of topdressing is; Topdress: drench on March 9 and March 22 and execute urea 10kg/ mu, it is 1:100 that concentration is executed in pouring for the first time, and it is 1:50 that concentration is executed in pouring for the second time; Transplant rear 10 days every mu of points and execute domestic composite fertilizer (15-15-15) 15kg, transplant rear 25 days every mu of points and execute domestic composite fertilizer (15-15-15) 25kg.
Process four, blank (the full phase is not executed any fertilizer).
5. test-results:
(1) use biological organic fertilizer of the present invention to the impact of Growth of Brassica Parachinensis
Observe in process of the test, use the processing one of biological organic fertilizer of the present invention, cabbage heart leaf look dark green, and growing way is good, and blade is wider and thick, tender flower stalk is thicker, from the data presentation of table 4 every 7 days, the plant height increasing degree of processing a cabbage heart is 56.3%, 69.3% and 85.8%, and the width of blade increasing degree is respectively 46.4%, 78.0% and 32.9%, the blade length increasing degree is respectively 48.6%, 63.5% and 89.4%, and the thick increasing degree of stem is respectively 66.7%, 80.0% and 88.9%; The plant height increasing degree of processing two cabbage hearts is 54.2%, 67.6% and 84.7%, the width of blade increasing degree is respectively 42.9%, 75.0% and 31.4%, the blade length increasing degree is respectively 45.7%, 62.7% and 86.7%, and the thick increasing degree of stem is respectively 33.3%, 75.0% and 85.7%; The plant height increasing degree of processing three cabbage hearts is 50.0%, 66.7% and 82.5%, the width of blade increasing degree is respectively 35.7%, 71.7% and 29.2%, the blade length increasing degree is respectively 40.0%, 53.1% and 85.3%, and the thick increasing degree of stem is respectively 33.3%, 50.0% and 83.3%; The plant height increasing degree of processing four cabbage hearts is 47.9%, 66.2% and 74.6%, the width of blade increasing degree is respectively 32.17%, 70.3% and 27.0%, the blade length increasing degree is respectively 34.3%, 51.1% and 84.5%, and the thick increasing degree of stem is respectively 33.3%, 25.0% and 60.0%.
During results, process a cabbage heart plant height, width of blade, length and stem rough segmentation not than the increase by 3.1%, 5.4%, 3.9% and 30.8% of processing two, than the increase by 7.8%, 15.5%, 15.8%, 54.5% of processing three; Than the increase by 14.6%, 21.3%, 22.9% and 112.5% of processing four.Presentation of results is used biological organic fertilizer of the present invention and can be promoted Growth of Brassica Parachinensis, increases cabbage heart plant height, width of blade, length and stem thick.
Table 4 is used biological organic fertilizer of the present invention to the impact (unit: cm) of Growth of Brassica Parachinensis
(2) use biological organic fertilizer of the present invention to the impact of yield of flowering Chinese cabbage
As can be seen from Table 5, between processing one and processing two, processing three, processing four, volume variance reaches utmost point conspicuous level.Process an output the highest, process two, three and take second place, process four output minimum.
The processing one residential quarter mean yield that cabbage heart is used biological organic fertilizer of the present invention is 50.4kg, folding per mu yield 1680.0kg, and than processing two mu of volume increase 120.0kg, amplification is 7.7%, increases production extremely remarkable; Than processing three mu of volume increase 253.3kg, amplification is 17.8%, increases production extremely remarkable; Than processing four mu of volume increase 283.3kg, amplification is 20.3%, increases production extremely remarkable.Presentation of results is compared with processing two, three, four, and it is obvious that cabbage heart is used biological organic fertilizer production-increasing function of the present invention.
Table 5 is used biological organic fertilizer of the present invention to the impact of yield of flowering Chinese cabbage
The different lowercases in data back in table are test significant difference through LSR, and different capitalizations are extremely remarkable through LSR test difference.
Conclusion (of pressure testing): compare with the blank that does not apply fertilizer with the biological organic fertilizer of using deactivation, use biological organic fertilizer of the present invention and can promote Growth of Brassica Parachinensis, increase cabbage heart plant height, width of blade, length and stem thick, compared with the control, use biological organic fertilizer of the present invention and can promote the cabbage heart volume increase.
7 kinds of application experiments of planting shatian pomelo of embodiment
1. test site: test in old wind gap reservoir orchard, Taihe county village, Wuhua County of Guangdong Province east of a river town, is experimental field mountain red earth.
2. test kind: shatian pomelo.
3. for examination fertilizer: the biological organic fertilizer that the embodiment of the present invention 3 prepares, living bacteria count are not less than 0.3 hundred million cuf/g, You Ji Zhi＞=30%, N+P
2O
5+ K
2O＞=4%.
4. 4 processing are established in test, and each is processed 3 times and repeats, random alignment, and each residential quarter selects the close Shatian Pomelo strain of 4 strain growing ways to test.Each Treatment Design following (specifically with reference to embodiment 5 Treatment Design):
Process one, use biological organic fertilizer+custom and topdress;
Process two, use the biological organic fertilizer of deactivation+custom and topdress;
Process three, conventional contrast (custom base manure+custom is topdressed);
Process four, blank (the full phase is not executed any fertilizer).
Shatian pomelo is planted in spring sowing in 1998, approximately 25 strains of mu tree planting, and on November 15th, 2011 gathered in the crops.it is as follows that each processes the fertilising situation: process one and make short fertilizers for potted flowers in ditch spread 4.0kg on March 5 in 2011 biological organic fertilizer/strain of the present invention and 1.0kg45% compound potassium sulfate fertilizer (18-10-17)/strain, 1.0kg biological organic fertilizer/strain of the present invention is executed in pouring on April 8 and 0.5kg compound potassium sulfate fertilizer (18-10-17) thanks to fertilizers for potted flowers, the phase of bearing fruit May 18 is used 1.0kg biological organic fertilizer/strain of the present invention and 2.0kg compound potassium sulfate fertilizer (18-10-17)/strain, use 1.0kg biological organic fertilizer/strain of the present invention before fruit picking on October 9, use 2.0kg biological organic fertilizer/strain of the present invention and 2.0kg compound potassium sulfate fertilizer (18-10-17)/strain after fruit picking on November 27, process two and use the biological organic fertilizer of deactivation one same period with processing, process three and use the peanut press pulp that becomes thoroughly decomposed a same period with processing, the four full phases of processing are not used any fertilizer.
5. test-results:
(1) use biological organic fertilizer of the present invention to the impact of shatian pomelo Main Agronomic shape
As seen from Table 6, process one and bear fruit number respectively than processing two, three, four increases by 6.6%, 2.1%, 13.3%; During results, processing one really footpath increases by 3.9%, 5.4%, 11.7% than processing two, three, four respectively; The single fruit Mean Fruit Weight increases by 5.9%, 5.1%, 13.0% than processing two, three, four respectively; Soluble solid increases by 0.9%, 0.3%, 2.0% than processing two, three, four respectively.Presentation of results, use biological organic fertilizer of the present invention can increase shatian pomelo bear fruit the number and single fruit on average heavily wait.
Table 6 is used biological organic fertilizer of the present invention to the impact of shatian pomelo Other Main Agronomic Characters
(2) use biological organic fertilizer of the present invention to the impact of shatian pomelo output
By as seen from Table 7, process a shatian pomelo output and reach conspicuous level with processing two, three, four cell production differences.Process a shatian pomelo output the highest, process three and two and take second place, process four output minimum.The processing one shatian pomelo residential quarter mean yield of using biological organic fertilizer of the present invention is 475.4kg, and folding per mu yield 2859.4kg increases 327.5kg than processing two mu, and amplification 12.9% is increased production extremely remarkable; Increase 194.4kg, amplification 7.3%, increase yield significantly than processing three; Increase 626.3kg than processing four mu, amplification 28.0% is increased production extremely remarkable.Presentation of results biological organic fertilizer of the present invention has more significant production-increasing function to shatian pomelo.
Table 7 is used biological organic fertilizer of the present invention to the impact of shatian pomelo output
The different lowercases in data back in table are test significant difference through LSR, and different capitalizations are extremely remarkable through LSR test difference.
Conclusion (of pressure testing): compare with the blank that does not apply fertilizer with the biological organic fertilizer of using deactivation, using biological organic fertilizer of the present invention can increase bear fruit number and single fruit of shatian pomelo and on average weigh, compare with the routine contrast of habits fertilization, use that biological organic fertilizer of the present invention can increase shatian pomelo fruit footpath and single fruit is on average heavy, shatian pomelo mu increase yield significantly.
8 kinds of application experiments of planting shatian pomelo of embodiment
1. test site: test is carried out in orchard, village, southwest, stone fan town, Mei County, Guangdong Province, is experimental field red soil, middle fertility.
2. test kind: shatian pomelo.
3. for examination fertilizer: biological organic fertilizer of the present invention, living bacteria count are not less than 0.3 hundred million cuf/g, You Ji Zhi＞=30%, N+P
2O
5+ K
2O＞=4%.
4. 4 processing are established in test, and each is processed 3 times and repeats, random alignment, and each residential quarter selects the close Shatian Pomelo strain of 4 strain growing ways to test.Each Treatment Design following (specifically with reference to embodiment 5 Treatment Design):
Process one, use biological organic fertilizer+custom and topdress;
Process two, use the biological organic fertilizer of deactivation+custom and topdress;
Process three, conventional contrast (custom base manure+custom is topdressed);
Process four, blank (the full phase is not executed any fertilizer).
Shatian pomelo was planted on March 15th, 1994, approximately 22 strains of mu tree planting, and on November 6th, 2011 gathered in the crops.It is as follows that each processes the fertilising situation: all execute 9.1kg fertilizer pig manure and 1.7kg potassium sulfate type Sa Kefu composite fertilizer (17-17-17) makes base manure in strain on November 23 in 2010, custom is topdressed minutes for 5 times.wherein 0.5kg urea and the short fertilizers for potted flowers of 0.5kg potassium sulfate type Sa Kefu composite fertilizer (17-17-17) work are executed in strain on January 25 in 2011, 0.5kg urea is executed in strain on April 18 in 2011 and 1.0kg potassium sulfate type Sa Kefu composite fertilizer (17-17-17) makes steady fruit fertilizer, strain on May 16 in 2011 is executed 1.0kg potassium sulfate type Sa Kefu composite fertilizer (17-17-17) and is made fruit-promoting fertilizer, strain on June 25 in 2011 is executed 1.0kg potassium sulfate type Sa Kefu composite fertilizer (17-17-17) and is made fruit-promoting fertilizer, 1.0kg potassium sulfate type Sa Kefu composite fertilizer (17-17-17) is executed in strain on July 28 in 2011 and the 0.5kg vitriolate of tartar is made fruit-promoting fertilizer.
The full phase of processing is used 9.0kg biological organic fertilizer/strain altogether, wherein short fertilizers for potted flowers is made in ditch spread 4.0kg on January 25th, 2011 biological organic fertilizer of the present invention/strain, pouring on April 18 is executed 1.0kg biological organic fertilizer/strain of the present invention and is thanked to fertilizers for potted flowers, 1.0kg biological organic fertilizer/strain of the present invention and 2.0kg biological organic fertilizer/strain of the present invention are executed in May 16, each pouring of fruit expanding period on June 25, and fruit picking on September 25 was opened shallow ridges in front 40 days and used 1.0kg biological organic fertilizer/strain of the present invention; Process two and use the biological organic fertilizer of deactivation one same period with processing; Processing three uses base manure and topdresses according to the locality custom; The four full phases of processing are not used any fertilizer.
5. test-results:
(1) use biological organic fertilizer of the present invention to the impact of shatian pomelo Main Agronomic shape
Process of the test is observed, and shatian pomelo uses that biological organic fertilizer posterior lobe look of the present invention is dark green, and blade is thicker, falls leaves less.As seen from Table 8, after processing one flower thinning, the female flower number increases by 1.3%, 1.2%, 2.6% than processing two, three, four respectively; The number of bearing fruit increases by 2.8%, 8.0%, 129.0% than processing two, three, four respectively; During results, processing a fruit footpath ratio processing two, four increases by 4.3%, 65.5%, reduces 1.4% than processing three; Single fruit on average increases by 8.3%, 4.0%, 73.3% than processing two, three, four heavily respectively.Soluble solid improves 0.6%, 0.2%, 4.3% than processing two, three, four respectively.Presentation of results is used the single fruit that biological organic fertilizer of the present invention can increase shatian pomelo on average heavy, compares with the routine of habits fertilization, and using biological organic fertilizer of the present invention can increase the number of bearing fruit.
Table 8 is used biological organic fertilizer of the present invention to the impact of shatian pomelo Other Main Agronomic Characters
(2) use biological organic fertilizer of the present invention to the impact of shatian pomelo output
By as seen from Table 9, process a shatian pomelo output and reach utmost point conspicuous level with processing two, three, four cell production differences.Process a shatian pomelo output the highest, process two and three and take second place, process four output minimum.The processing one shatian pomelo residential quarter mean yield of using biological organic fertilizer of the present invention is 774.4kg, and folding per mu yield 4259.2kg increases 431.2kg than processing two mu, and amplification 11.3% is increased production extremely remarkable; Than processing three mu of volume increase 3186.7kg, amplification 297.1% is increased production extremely remarkable; Than processing four mu of volume increase 3186.7kg, amplification 297.1% is increased production extremely remarkable.Presentation of results biological organic fertilizer of the present invention has more significant production-increasing function to shatian pomelo.
Table 9 is used biological organic fertilizer of the present invention to the impact of shatian pomelo output
The different lowercases in data back in table are test significant difference through LSR, and different capitalizations are extremely remarkable through LSR test difference.
Conclusion (of pressure testing): using biological organic fertilizer of the present invention can significantly increase shatian pomelo output.Compare with the blank that does not apply fertilizer with the biological organic fertilizer of using deactivation, using biological organic fertilizer of the present invention, can to increase the shatian pomelo single fruit on average heavy, compares with the routine contrast of habits fertilization, and using biological organic fertilizer of the present invention can increase the shatian pomelo number of bearing fruit.
9 kinds of application experiments of planting lichee of embodiment
1. test site: test is carried out respectively in orchard, husky 4, area under control of field in Shi Li village, Guan Zhu town, Maoming Dianbai County.Be all low mountain, hills red earth.
2. test kind: lichee (white sugar small-mouthed jar).
3. for examination fertilizer: biological organic fertilizer of the present invention, living bacteria count are not less than 0.3 hundred million cuf/g, You Ji Zhi＞=30%, N+P
2O
5+ K
2O＞=4%.
4. test orchard situation is as shown in table 10:
Table 10
The average strain in orchard 1 is produced 18.75 kilograms, and 468.8 kilograms of per mu yields, strain are produced respectively and increased production 120% and 460% than orchard 3 and orchard 4, and per mu yield increases 87.5% and 300%; Orchard 2 strains are produced and are increased 77.8% and 344.7% than orchard 3 and orchard 4, and per mu yield increases 77.9% and 300% than orchard 3 and orchard 4.
The application experiment of embodiment 10 rice cultivations
The experimental plot is in field, field roadside, the pool, nine groups of big cities, Xin Tangxiang Shan Bian village, Guangzhou.Soil type is field at the bottom of pellet in a shotgun cartridge, and organic content is lower, and paddy rice is the excellent perfume (or spice) of spoke.
The experimental plot in August 8 the rice transplanting mode transplant seedlings, establish 0.5 mu of test site, use 25 kilograms of biological organic fertilizers of the present invention before rice transplanting, folding mu is executed 50 kilograms.0.52 mu of check plot area is executed 20 kilograms of carbon ammoniums, and folding mu is executed 38.5 kilograms, 30 kilograms, fused(calcium magnesium)phosphate, and folding mu is executed 59.6 kilograms.Build ridge between two processing and be separated by, perpendicular board is expressed.Each is distinguished fertilizer and keeps shoaling layer to spread down, harrows even rice transplanting.
Field management: keep shoaling layer to turn green after rice transplanting.August 16,5 kilograms, urea is executed in 0.5 mu of test site, executes 6.2 kilograms of import composite fertilizers (nitrogen 15, phosphorus 15, potassium 15); 5.2 kilograms, urea is executed in 0.52 mu of check plot, 6.5 kilograms of import composite fertilizers.Each treatment zone folding mu is executed 10 kilograms, urea, and 12.5 kilograms of composite fertilizers do disposable topdressing and use sufficient.Water layer falls driedly naturally after enough seedlings, does wet the management to booting mid-term.Rear drawing water for twice because of the autumn drought solves the heading water.Insect pest preventing and controlling in August 28, September 11 and each integrated control on October 22 once.November 10 ripe and harvested, totally 105 days breeding time of Honda.
Check and accept the front peasant household that invites and participate in stochastic sampling, one square metre of test site sampling, effectively fringe 312 fringes, roll over effectively fringe 208010 fringes of mu; One square metre of check plot stochastic sampling, effectively fringe 309 fringes, roll over effectively fringe 206010 fringes of mu.Each treatment zone harvesting on November 10 is participated in by peasant household and the villager that passes by on one's way, and 10 square metres of stochastic sampling harvestings are as shown in table 11 below:
Table 11 is used biological organic fertilizer experimental result of the present invention
Test Summary: the test site seedlings of cereal crops were just showed more dark green than the check plot after rice transplanting on the 12nd day, and fertility is fast, and precocious 2～3 days, and during being continued until Heading filling.Biological organic fertilizer of the present invention, fertilizer have soy sauce fragrance, and be not smelly, and peasant household likes using, and late rice is used and obtains 33.3 kilograms of mu volume increase, and amplification is 7.03%, and benefit is very remarkable; After using fertilizer of the present invention, the soil organism has a certain amount of increase, and arable soil colloid and crumb structure are played a protective role.Use biological organic fertilizer of the present invention, can effectively reduce the pollution to ploughing of chemical fertilizer, adhere to life-time service, can progressively repair the soil of degenerating because of long-term single use chemical fertilizer.
Comparative Examples 1
With bacillus megaterium (available from Guangdong Province microbiological analysis inspection center, Article Number is ACCC11011), silicate bacteria is (available from Agricultural University Of South China, Article Number is IM6), vinelandii are (available from Guangdong Province microbiological analysis inspection center, Article Number is AS1.222) render in inosine mother liquor nutritive medium and cultivate, make in every milliliter of microbial nutrient solution and contain: phosphate solubilizing bacteria 0.1 hundred million cuf/mL, potassium solubilizing bacteria 0.1 hundred million cuf/mL, vinelandii 0.1 hundred million cuf/mL.(do not need camera bellows) and cultivate the microbial nutrient solution that namely obtained containing various trace elements in 50～60 days under 25～30 ℃.Above-mentioned bacterial strains is not limited to described purchasing channel, also can be self-control or other sources.Inosine mother liquor nutritive medium mainly contains organic matter, nitrogen, phosphorus, potassium, humic acid and glucose.
Preparation biological organic fertilizer step is as follows:
S1. with 50 parts, rice bran, 20 parts of dregs of beans, get mixture after 20 parts of raw material blendings such as grade of 10 parts of peanut press pulps and Semen Maydis powder, mixture is tiled, the thickness of preferred tiling is 10cm, press the mixture (guaranteeing that the mixed material of liquid surrounds) that 1Kg:0.1L inosine mother liquor nutritive medium soaks tiling, add yeast (available from Guangdong Province microbiological analysis inspection center in mixture, Article Number is AS2.180, add-on is 6L), be maintained at 55～60 ℃ of fermentations, ferment and after 5～7 days, starting material are carried out turning, later on every turning in 1～3 month 1 time, continuing fermentation 5～6 months, the mixture slaking is decomposed,
The temperature of the mixture that after the described fermentation of S2.S1 is completed, slaking is decomposed is down to 26～30 ℃, gross weight according to mixture: the volume of microbial nutrient solution is that 10Kg:2L adds microbial nutrient solution, microorganism in microbial nutrient solution bred 10～20 days in the mixture that slaking is decomposed, and got biological organic fertilizer.
Comparative Examples 2
With bacillus megaterium (available from Guangdong Province microbiological analysis inspection center, Article Number is ACCC11011), silicate bacteria is (available from Agricultural University Of South China, Article Number is IM6), vinelandii are (available from Guangdong Province microbiological analysis inspection center, Article Number is AS1.222) render in inosine mother liquor nutritive medium and cultivate, make in every milliliter of microbial nutrient solution and contain: phosphate solubilizing bacteria 0.1 hundred million cuf/mL, potassium solubilizing bacteria 0.1 hundred million cuf/mL, vinelandii 0.1 hundred million cuf/mL.(do not need camera bellows) and cultivate the microbial nutrient solution that namely obtained containing various trace elements in 50～60 days under 25～30 ℃.Above-mentioned bacterial strains is not limited to described purchasing channel, also can be self-control or other sources.Inosine mother liquor nutritive medium mainly contains organic matter, nitrogen, phosphorus, potassium, humic acid and glucose.
Preparation biological organic fertilizer step is as follows:
S1. with 90 parts, rice bran, 5 parts of dregs of beans, get mixture after 5 parts of raw material blendings such as grade of 5 parts of peanut press pulps and bean dregs, mixture is tiled, the thickness of preferred tiling is 10cm, press the mixture (guaranteeing that the mixed material of liquid surrounds) that 1Kg:0.1L inosine mother liquor nutritive medium soaks tiling, add yeast (available from Guangdong Province microbiological analysis inspection center in mixture, Article Number is AS2.180, add-on is 6L), be maintained at 55～60 ℃ of fermentations, ferment and after 5～7 days, starting material are carried out turning, later on every turning in 1～3 month 1 time, continuing fermentation 5～6 months, the mixture slaking is decomposed,
The temperature of the mixture that after the described fermentation of S2.S1 is completed, slaking is decomposed is down to 26～30 ℃, gross weight according to mixture: the volume of microbial nutrient solution is that 10Kg:2L adds microbial nutrient solution, microorganism in microbial nutrient solution bred 10～20 days in the mixture that slaking is decomposed, and got biological organic fertilizer.
Comparative Examples 3
With bacillus megaterium (available from Guangdong Province microbiological analysis inspection center, Article Number is ACCC11011), silicate bacteria is (available from Agricultural University Of South China, Article Number is IM6), vinelandii are (available from Guangdong Province microbiological analysis inspection center, Article Number is AS1.222) render in inosine mother liquor nutritive medium and cultivate, make in every milliliter of microbial nutrient solution and contain: phosphate solubilizing bacteria 0.05 hundred million cuf/mL, potassium solubilizing bacteria 0.05 hundred million cuf/mL, vinelandii 0.05 hundred million cuf/mL.Cultivation obtained microbial nutrient solution in 30 days (not need camera bellows) under 25～30 ℃.Above-mentioned bacterial strains is not limited to described purchasing channel, also can be self-control or other sources.Inosine mother liquor nutritive medium mainly contains organic matter, nitrogen, phosphorus, potassium, humic acid and glucose.
Preparation biological organic fertilizer step is as follows:
S1. with after the raw material blendings such as 5 parts, 60 parts, rice bran, 5 parts of dregs of beans, 5 parts of peanut press pulps and bean dregs mixture, mixture is tiled, the thickness of preferred tiling is 10cm, press the mixture (guaranteeing that the mixed material of liquid surrounds) that 1Kg:0.05L inosine mother liquor nutritive medium soaks tiling, add yeast (available from Guangdong Province microbiological analysis inspection center in mixture, Article Number is AS2.180, add-on is 6L), be maintained at 55～60 ℃ of fermentations, ferment and after 3 days, starting material are carried out turning, continuing fermentation 5～6 months decomposes the mixture slaking;
The temperature of the mixture that after the described fermentation of S2.S1 is completed, slaking is decomposed is down to 40 ℃, gross weight according to mixture: the volume of microbial nutrient solution is that 10Kg:1L adds microbial nutrient solution, microorganism in microbial nutrient solution bred 10～20 days in the mixture that slaking is decomposed, and got biological organic fertilizer.
The fertilizer that Comparative Examples 4 Comparative Examples prepare and fertilizer of the present invention are the application contrast experiment of plantation cabbage heart
1. test site: test is carried out in the Da Dong of Fengcheng neighbourhood committee food market, Shaoguan City of Guangdong Province Xinfeng County, and experimental field soil property is silty loam, middle fertility, and preceding crop is fresh kidney beans.
2. test kind: slow cabbage heart.
3. for examination fertilizer: biological organic fertilizer of the present invention, living bacteria count are not less than 0.3 hundred million cuf/g, and organic matter is not less than 47%, N+P
2O
5+ K
2O＞=4%.
4. 4 processing are established in test, and each is processed 3 times and repeats, random alignment, residential quarter area 20m
2Cabbage heart is in sowing on December 30, and transplant January 30 next year, and mu is planted 2.5 ten thousand strains, and March 22, results were complete.Each Treatment Design is as follows:
Process one, before cabbage heart is transplanted, mu is executed 80kg biological organic fertilizer of the present invention (biological organic fertilizer of selecting the embodiment of the present invention 1 to prepare is used) and made base manure, topdresses: each drenched and executed biological organic fertilizer 10kg/ mu February 6 and February 19; Transplant rear 10 days every mu of points and execute three element compound fertilizer (15-15-15) 15kg, transplant rear 25 days every mu of points and execute three element compound fertilizer (15-15-15) 25kg.
Process two, before cabbage heart is transplanted, mu is executed 80kg biological organic fertilizer of the present invention (biological organic fertilizer of selecting Comparative Examples 1 to prepare is used) and made base manure, topdresses: each drenched and executed the biological organic fertilizer 10kg/ mu that ratio 1 prepares February 6 and February 19; Transplant rear 10 days every mu of points and execute three element compound fertilizer (15-15-15) 15kg, transplant rear 25 days every mu of points and execute three element compound fertilizer (15-15-15) 25kg.
Process three, before cabbage heart is transplanted, mu is executed 80kg biological organic fertilizer of the present invention (biological organic fertilizer of selecting Comparative Examples 2 to prepare is used) and made base manure, topdresses: each drenched and executed the biological organic fertilizer 10kg/ mu that Comparative Examples 2 prepares February 6 and February 19; Transplant rear 10 days every mu of points and execute three element compound fertilizer (15-15-15) 15kg, transplant rear 25 days every mu of points and execute three element compound fertilizer (15-15-15) 25kg.
Process four, before cabbage heart is transplanted, mu is executed 80kg biological organic fertilizer of the present invention (biological organic fertilizer of selecting Comparative Examples 3 to prepare is used) and made base manure, topdresses: each drenched and executed the biological organic fertilizer 10kg/ mu that Comparative Examples 3 prepares February 6 and February 19.
Process five, blank (the full phase is not executed any fertilizer).
5. test-results:
The data that stages cabbage heart plant height, width of blade, length and stem are thick from table 12 can find out, the fertilizer efficiency of biological organic fertilizer of the present invention is well more a lot of than the fertilizer efficiency of the standby biological organic fertilizer that obtains of Comparative Examples.
Table 12 is used biological organic fertilizer of the present invention and Comparative Examples biological organic fertilizer to the impact (unit: cm) of Growth of Brassica Parachinensis
Claims (10)
1. the preparation method of a biological organic fertilizer, is characterized in that, comprises the following steps:
S1. rice bran, dregs of beans, peanut press pulp and bean dregs are mixed in proportion to get mixture, with the mixture tiling, the mixture with after inosine mother liquor nutritive medium immersion tiling adds yeast, in 50～60 ℃ of bottom fermentations, carries out turning in fermenting process and processes;
After the described fermentation of S2.S1 is completed, the temperature of mixture is down to 26～30 ℃, adds microbial nutrient solution, the microorganism in microbial nutrient solution bred in mixture 10～20 days, namely got described biological organic fertilizer;
Wherein, in the described mixture of S1, rice bran, dregs of beans, peanut press pulp and bean dregs parts by weight are: rice bran 60～80 weight parts, dregs of beans 5～15 weight parts, peanut press pulp 5～15 weight parts, bean dregs 5～15 weight parts;
Contain phosphate solubilizing bacteria 0.1～0.3 hundred million cuf/mL, potassium solubilizing bacteria 0.1～0.3 hundred million cuf/mL and vinelandii 0.1～0.3 hundred million cuf/mL in the described microbial nutrient solution of S2.
2. the preparation method of biological organic fertilizer according to claim 1, is characterized in that in the described fermenting process of S1, adds the inedible oil fat that accounts for mixture quality 1.5～2% after the 5th～7 day; Described inedible oil fat is glycerine or edible waste oil.
3. the preparation method of biological organic fertilizer according to claim 1, is characterized in that, the consumption of the described inosine mother liquor of S1 nutritive medium is according to the gross weight of mixture: the volume of inosine mother liquor nutritive medium is that 10Kg:1L determines;
The consumption of the described microbial nutrient solution of S2 is according to the gross weight of mixture: the volume of microbial nutrient solution is that 10Kg:2L determines.
4. the preparation method of biological organic fertilizer according to claim 1, is characterized in that containing in the described microbial nutrient solution of S2: phosphate solubilizing bacteria 0.2 hundred million cuf/mL microbial nutrient solutions; Potassium solubilizing bacteria 0.2 hundred million cuf/mL microbial nutrient solutions and vinelandii 0.2 hundred million cuf/mL microbial nutrient solutions.
5. the preparation method of biological organic fertilizer according to claim 1, is characterized in that, in the described microbial nutrient solution of S2, described phosphate solubilizing bacteria be bacillus megaterium (
Bacillus megaterium), described potassium solubilizing bacteria be silicate bacteria (
Silicate bacteria), described vinelandii be vinelandii (
Azotobacter sp).
6. the preparation method of biological organic fertilizer according to claim 5, it is characterized in that, the preparation method of the described microbial nutrient solution of S2 is: bacillus megaterium, silicate bacteria, vinelandii are rendered in inosine mother liquor nutritive medium, cultivated 50～60 days and get final product in 20～30 ℃.
7. the preparation method of biological organic fertilizer according to claim 1, is characterized in that, it is after 5～7 days, mixture to be carried out turning for the first time in fermentation that the described turning of S1 is processed, after turning for the first time every turning in 1～3 month once; The time of described fermentation is 5～6 months.
8. the preparation method of biological organic fertilizer according to claim 7, is characterized in that, S1 is described adds the inedible oil fat that accounts for mixture quality 1.5～2% when fermentation was carried out turning for the first time to mixture after 5～7 days; Described inedible oil fat is glycerine or edible waste oil.
9. the biological organic fertilizer for preparing of the described preparation method of claim 1 to 8 any one.
10. the application of the described biological organic fertilizer of claim 9 aspect plantation lichee, cabbage heart, shatian pomelo or paddy rice.
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| CN104098411A (en) * | 2014-07-29 | 2014-10-15 | 南京正美实农化有限公司 | Modified carbamide, micromolecule active organic matter and preparation method for modified carbamide |
| CN104140316A (en) * | 2014-07-04 | 2014-11-12 | 马鞍山市四季果业有限公司 | Organic fertilizer capable of improving stress resistance of plants and preparation method of organic fertilizer |
| CN104725083A (en) * | 2013-12-19 | 2015-06-24 | 王骁 | Flower fertilizer and preparation method thereof |
| CN104938280A (en) * | 2015-06-18 | 2015-09-30 | 陈祺 | Shatian pomelo cultivating method with high yield effect in successive years |
| CN105111012A (en) * | 2015-07-31 | 2015-12-02 | 区燕丽 | Special fertilizer for Shatian pomelos |
| CN105850686A (en) * | 2016-05-06 | 2016-08-17 | 钦州市长盛农业有限公司 | Method for achieving aerosol cultivation and suspension tuber formation of Chinese yams |
| CN106631432A (en) * | 2016-12-12 | 2017-05-10 | 新昌县奥而特农业科技有限公司 | Organic fertilizer for pomegranates and preparation method thereof |
| CN108658633A (en) * | 2018-07-28 | 2018-10-16 | 浙江国禾实业股份有限公司 | There is the ground fertilizer of machine operation |
| CN109438020A (en) * | 2018-12-30 | 2019-03-08 | 隆安县清水湾农业有限责任公司 | A kind of cabbage heart fertilizer special for organic and preparation method thereof |
| CN111217635A (en) * | 2019-11-26 | 2020-06-02 | 四川宸煜林业开发有限责任公司 | Cinnamomum camphora leaf bio-organic fertilizer and preparation method thereof |
| CN113214032A (en) * | 2021-06-11 | 2021-08-06 | 四川中农润泽生物科技有限公司 | Organic fertilizer for soil remediation and production process thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1389438A (en) * | 2002-07-18 | 2003-01-08 | 杨雄 | Plant organic liquid fertilizer |
| CN1434004A (en) * | 2003-03-11 | 2003-08-06 | 赵鹏 | Biological organic-inorganic compound fertilizer and productive method thereof |
| CN101113120A (en) * | 2007-07-09 | 2008-01-30 | 北京双色港农业科技开发有限公司 | Nano biological fertilizer |
| CN101508601A (en) * | 2009-03-31 | 2009-08-19 | 孙明坤 | Microorganism organic fertilizer and method of preparing the same |
-
2013
- 2013-02-05 CN CN201310045294.5A patent/CN103086771B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1389438A (en) * | 2002-07-18 | 2003-01-08 | 杨雄 | Plant organic liquid fertilizer |
| CN1434004A (en) * | 2003-03-11 | 2003-08-06 | 赵鹏 | Biological organic-inorganic compound fertilizer and productive method thereof |
| CN101113120A (en) * | 2007-07-09 | 2008-01-30 | 北京双色港农业科技开发有限公司 | Nano biological fertilizer |
| CN101508601A (en) * | 2009-03-31 | 2009-08-19 | 孙明坤 | Microorganism organic fertilizer and method of preparing the same |
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| CN104725083A (en) * | 2013-12-19 | 2015-06-24 | 王骁 | Flower fertilizer and preparation method thereof |
| CN104140316A (en) * | 2014-07-04 | 2014-11-12 | 马鞍山市四季果业有限公司 | Organic fertilizer capable of improving stress resistance of plants and preparation method of organic fertilizer |
| CN104098411A (en) * | 2014-07-29 | 2014-10-15 | 南京正美实农化有限公司 | Modified carbamide, micromolecule active organic matter and preparation method for modified carbamide |
| CN104938280A (en) * | 2015-06-18 | 2015-09-30 | 陈祺 | Shatian pomelo cultivating method with high yield effect in successive years |
| CN105111012A (en) * | 2015-07-31 | 2015-12-02 | 区燕丽 | Special fertilizer for Shatian pomelos |
| CN105850686A (en) * | 2016-05-06 | 2016-08-17 | 钦州市长盛农业有限公司 | Method for achieving aerosol cultivation and suspension tuber formation of Chinese yams |
| CN105850686B (en) * | 2016-05-06 | 2019-02-05 | 钦州市长盛农业有限公司 | The method that Chinese yam aerial fog cultivation vacantly ties potato |
| CN106631432A (en) * | 2016-12-12 | 2017-05-10 | 新昌县奥而特农业科技有限公司 | Organic fertilizer for pomegranates and preparation method thereof |
| CN108658633A (en) * | 2018-07-28 | 2018-10-16 | 浙江国禾实业股份有限公司 | There is the ground fertilizer of machine operation |
| CN109438020A (en) * | 2018-12-30 | 2019-03-08 | 隆安县清水湾农业有限责任公司 | A kind of cabbage heart fertilizer special for organic and preparation method thereof |
| CN111217635A (en) * | 2019-11-26 | 2020-06-02 | 四川宸煜林业开发有限责任公司 | Cinnamomum camphora leaf bio-organic fertilizer and preparation method thereof |
| CN113214032A (en) * | 2021-06-11 | 2021-08-06 | 四川中农润泽生物科技有限公司 | Organic fertilizer for soil remediation and production process thereof |
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