CN102351578B - Soluble straw decomposing agent, preparation method and application thereof - Google Patents
Soluble straw decomposing agent, preparation method and application thereof Download PDFInfo
- Publication number
- CN102351578B CN102351578B CN 201110199404 CN201110199404A CN102351578B CN 102351578 B CN102351578 B CN 102351578B CN 201110199404 CN201110199404 CN 201110199404 CN 201110199404 A CN201110199404 A CN 201110199404A CN 102351578 B CN102351578 B CN 102351578B
- Authority
- CN
- China
- Prior art keywords
- soluble
- straw
- agent
- straw decomposing
- decomposing agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000010902 straw Substances 0.000 title claims abstract description 234
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 187
- 238000002360 preparation method Methods 0.000 title claims abstract description 45
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 102
- 239000000843 powder Substances 0.000 claims abstract description 79
- 241000894006 Bacteria Species 0.000 claims abstract description 72
- 239000002131 composite material Substances 0.000 claims abstract description 35
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 8
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 8
- 239000007921 spray Substances 0.000 claims description 58
- 241000233866 Fungi Species 0.000 claims description 55
- 239000007788 liquid Substances 0.000 claims description 51
- 239000000725 suspension Substances 0.000 claims description 39
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 32
- 239000000203 mixture Substances 0.000 claims description 32
- 241000228245 Aspergillus niger Species 0.000 claims description 30
- 239000004615 ingredient Substances 0.000 claims description 26
- 230000000050 nutritive effect Effects 0.000 claims description 26
- 241000196324 Embryophyta Species 0.000 claims description 24
- 241000223259 Trichoderma Species 0.000 claims description 23
- 239000002054 inoculum Substances 0.000 claims description 20
- 238000000855 fermentation Methods 0.000 claims description 19
- 230000004151 fermentation Effects 0.000 claims description 19
- 238000002156 mixing Methods 0.000 claims description 19
- 240000006439 Aspergillus oryzae Species 0.000 claims description 18
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims description 18
- 241000194108 Bacillus licheniformis Species 0.000 claims description 17
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 16
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 16
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 claims description 16
- 229920002472 Starch Polymers 0.000 claims description 16
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 16
- 229910052796 boron Inorganic materials 0.000 claims description 16
- 229910052802 copper Inorganic materials 0.000 claims description 16
- 239000010949 copper Substances 0.000 claims description 16
- 229910052742 iron Inorganic materials 0.000 claims description 16
- 229910052750 molybdenum Inorganic materials 0.000 claims description 16
- 239000011733 molybdenum Substances 0.000 claims description 16
- 235000019698 starch Nutrition 0.000 claims description 16
- 239000008107 starch Substances 0.000 claims description 16
- 239000011701 zinc Substances 0.000 claims description 16
- 229910052725 zinc Inorganic materials 0.000 claims description 16
- 239000003337 fertilizer Substances 0.000 claims description 14
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 13
- 229910052748 manganese Inorganic materials 0.000 claims description 13
- 239000011572 manganese Substances 0.000 claims description 13
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims description 12
- 150000001413 amino acids Chemical class 0.000 claims description 12
- 239000004021 humic acid Substances 0.000 claims description 12
- 210000000582 semen Anatomy 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 10
- 229930006000 Sucrose Natural products 0.000 claims description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical group O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 9
- 239000012141 concentrate Substances 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 7
- 241000209051 Saccharum Species 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 4
- 238000009264 composting Methods 0.000 claims description 3
- 230000002538 fungal effect Effects 0.000 claims description 2
- 238000000354 decomposition reaction Methods 0.000 abstract description 47
- 238000005507 spraying Methods 0.000 abstract description 17
- 238000004945 emulsification Methods 0.000 abstract description 14
- 230000001580 bacterial effect Effects 0.000 abstract description 12
- 235000015097 nutrients Nutrition 0.000 abstract description 6
- 241000607479 Yersinia pestis Species 0.000 abstract description 5
- 201000010099 disease Diseases 0.000 abstract description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 5
- 230000015556 catabolic process Effects 0.000 abstract description 4
- 238000006731 degradation reaction Methods 0.000 abstract description 4
- 230000000443 biocontrol Effects 0.000 abstract 1
- 239000001913 cellulose Substances 0.000 abstract 1
- 229920002678 cellulose Polymers 0.000 abstract 1
- 238000000034 method Methods 0.000 description 109
- 230000008569 process Effects 0.000 description 95
- 238000012545 processing Methods 0.000 description 72
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 62
- 239000004202 carbamide Substances 0.000 description 62
- 238000012360 testing method Methods 0.000 description 44
- 230000000694 effects Effects 0.000 description 33
- 240000008042 Zea mays Species 0.000 description 22
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 22
- 240000007124 Brassica oleracea Species 0.000 description 21
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 21
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 21
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 21
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 21
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 21
- 235000009973 maize Nutrition 0.000 description 21
- 239000000243 solution Substances 0.000 description 21
- 244000005700 microbiome Species 0.000 description 16
- 239000000047 product Substances 0.000 description 16
- 239000002689 soil Substances 0.000 description 16
- 235000013311 vegetables Nutrition 0.000 description 16
- 244000221633 Brassica rapa subsp chinensis Species 0.000 description 14
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 14
- 239000012466 permeate Substances 0.000 description 13
- 239000002609 medium Substances 0.000 description 12
- 230000015572 biosynthetic process Effects 0.000 description 11
- 239000003995 emulsifying agent Substances 0.000 description 11
- 230000003413 degradative effect Effects 0.000 description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 8
- 230000008859 change Effects 0.000 description 8
- 210000003608 fece Anatomy 0.000 description 8
- 239000008103 glucose Substances 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- 239000005416 organic matter Substances 0.000 description 7
- 244000068988 Glycine max Species 0.000 description 6
- 235000010469 Glycine max Nutrition 0.000 description 6
- 240000007594 Oryza sativa Species 0.000 description 6
- 235000007164 Oryza sativa Nutrition 0.000 description 6
- 230000009286 beneficial effect Effects 0.000 description 6
- 229940041514 candida albicans extract Drugs 0.000 description 6
- 230000003203 everyday effect Effects 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 239000010871 livestock manure Substances 0.000 description 6
- 235000009566 rice Nutrition 0.000 description 6
- 239000008399 tap water Substances 0.000 description 6
- 235000020679 tap water Nutrition 0.000 description 6
- 238000010998 test method Methods 0.000 description 6
- 239000012138 yeast extract Substances 0.000 description 6
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- 238000013461 design Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000011591 potassium Substances 0.000 description 5
- 229910052700 potassium Inorganic materials 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- 240000002234 Allium sativum Species 0.000 description 4
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 4
- 230000009471 action Effects 0.000 description 4
- 239000013543 active substance Substances 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 235000004611 garlic Nutrition 0.000 description 4
- 238000003973 irrigation Methods 0.000 description 4
- 230000002262 irrigation Effects 0.000 description 4
- 238000009630 liquid culture Methods 0.000 description 4
- 239000002068 microbial inoculum Substances 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- 239000011343 solid material Substances 0.000 description 4
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 4
- 238000009423 ventilation Methods 0.000 description 4
- 101710099573 Casein kinase II subunit alpha Proteins 0.000 description 3
- 101710159482 Casein kinase II subunit alpha' Proteins 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- 230000009418 agronomic effect Effects 0.000 description 3
- 230000001186 cumulative effect Effects 0.000 description 3
- 230000000593 degrading effect Effects 0.000 description 3
- 230000003628 erosive effect Effects 0.000 description 3
- 244000037666 field crops Species 0.000 description 3
- 238000009863 impact test Methods 0.000 description 3
- 238000002513 implantation Methods 0.000 description 3
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 2
- 108010059892 Cellulase Proteins 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 241000726221 Gemma Species 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 241000223260 Trichoderma harzianum Species 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- 239000013522 chelant Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000011368 organic material Substances 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 239000000375 suspending agent Substances 0.000 description 2
- 235000015099 wheat brans Nutrition 0.000 description 2
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 description 1
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 235000000981 Brassica parachinensis Nutrition 0.000 description 1
- 241001604868 Brassica rapa var. parachinensis Species 0.000 description 1
- 240000001307 Myosotis scorpioides Species 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000005669 field effect Effects 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- -1 saponins compound Chemical class 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 244000000034 soilborne pathogen Species 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003971 tillage Methods 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Abstract
The invention provides a soluble straw decomposing agent, its preparation method and application. The soluble straw decomposing agent comprises by weight: 10-20 parts of a composite bacterial agent, 20-50 parts of nutrients, 2-5 parts of a plant source emulsification penetrant, and 25-68 parts of a soluble carrier. The composite bacterial agent contains cellulose decomposition bacteria, protein decomposition bacteria, and nutrient bio-control growth-promoting bacteria. The invention also provides a preparation method of the above soluble straw decomposing agent. The soluble straw decomposing agent provided in the invention is in a powder form and is fully soluble in water, and also can be applied through both scattering and spraying, thus being suitable for mechanized operation. The soluble straw decomposing agent provided in the invention can substantially speed up degradation of a plurality of crop straw, promote crop growth, and prevent a lot of crop diseases and pests.
Description
Technical field
The present invention relates to a kind of soluble straw decomposing agent, its and its preparation method and application, specifically, the present invention relates to a kind of is again can be entirely water-soluble straw decomposing inoculant of powder dose-type and preparation method thereof, and the application of this straw decomposing inoculant in the agricultural crop straw direct returning to farmland.The microorganism belonging to genus fertilizer field.
Background technology
Organic matter decomposing inoculant is a kind ofly can accelerate the microbial inoculant that organic materials (agricultural crop straw, feces of livestock and poultry, domestic refuse, municipal sludge etc.) is degraded and become thoroughly decomposed.Employed organic matter decomposing inoculant is insoluble powderous preparations and a small amount of water suspending agent mostly in the market.Organic matter decomposing inoculant is different according to contained microbe species, function and purposes, is divided into again can be used for the together organic matter decomposing inoculant and the straw decomposing inoculant that is directly used in crops straw returning field of composting such as agricultural crop straw and feces of livestock and poultry.Effective bacterium in the organic matter decomposing inoculant generally be by can be in fermentation or process of growth the composition of the microorganisms such as fungi, bacterium and actinomycetes of eccrine fiber element enzyme and proteolytic enzyme, but because composite microbe species is different, the characteristic of its product, function and difference on effect are very large.Especially straw decomposing inoculant owing to being the crops straw returning field that is directly used in the land for growing field crops, requires very high to various enzyme system's collocation and existence ability to function and the using method in the field of contained effective bacterium in the product.Existing organic matter decomposing inoculant as for existing straw decomposing inoculant, although product is different, also is insoluble powderous preparations mostly for compost entirely, can only manually spread fertilizer over the fields on the using method of field, can not spray, and more can not big area mechanize spray.In addition, these products have only been considered the Degradation of microorganism to stalk, ignored microorganism the field surely grow fast needed nutritive ingredient and to the promoter actions of this season farm crop and to the restraining effect of soil-borne pathogen.Although the organic materials water suspending agent can spray with mechanize the land for growing field crops direct labor and spray, but the effective bacterium in the product generally is bacterium and yeast etc., and there is not fungi, the fungi that plays a major role owing to the degraded to agricultural crop straw is difficult to prolonged preservation in water, so aqua limitation in the practical application of straw-returning is larger.Therefore, invent a kind of function comparatively complete, the pulvis that can the field spreads fertilizer over the fields, again can be the entirely water-soluble soluble straw decomposing agent, its that can machinery spray is very necessary.
Summary of the invention
Single and can not the direct labor or the shortcoming that sprays of mechanize for overcoming existing straw decomposing inoculant effect, the invention provides a kind of soluble straw decomposing agent, its.It is that powder dose-type again can be entirely water-soluble, is grouped into by following one-tenth by weight:
Composite fungus agent 10-20 part;
Nutritive ingredient 20-50 part;
Plant-sourced emulsification permeate agent 2-5 part;
Soluble carrier 25-68 part.
Preferably, soluble straw decomposing agent, its of the present invention is grouped into by following one-tenth by weight:
Composite fungus agent 12-18 part;
Nutritive ingredient 22-45 part;
Plant-sourced emulsification permeate agent 2-4 part;
Soluble carrier 33-64 part.
More preferably, soluble straw decomposing agent, its of the present invention is grouped into by following one-tenth by weight:
15 parts of composite fungus agents;
25 parts of nutritive ingredients;
3 parts of plant-sourced emulsification permeate agents;
57 parts of soluble carrier.
Contain cellulose-decomposing bacterium, protein decomposing dacteria, the short living bacterium of nutrition biological and ecological methods to prevent plant disease, pests, and erosion in the described composite fungus agent.
Cellulose-decomposing bacterium in the described composite fungus agent is trichoderma harziarum, aspergillus niger and aspergillus oryzae; Described protein decomposing dacteria is subtilis; The short living bacterium of described biological and ecological methods to prevent plant disease, pests, and erosion is Bacillus licheniformis.
Described composite fungus agent comprises trichoderma harziarum 0.2-1.0 hundred million cfu/g, aspergillus niger 0.5-2.0 hundred million cfu/g, aspergillus oryzae 0.5-2.0 hundred million cfu/g, subtilis 1-5 hundred million cfu/g, Bacillus licheniformis 1-5 hundred million cfu/g.Effectively the bacterium total content is 3-15 hundred million cfu/g.
Preferably, described composite fungus agent comprises trichoderma harziarum 0.5 hundred million cfu/g, aspergillus niger 100,000,000 cfu/g, aspergillus oryzae 100,000,000 cfu/g, subtilis 500,000,000 cfu/g, Bacillus licheniformis 500,000,000 cfu/g.Effective bacterium total content 〉=1,200,000,000 cfu/g.
Described composite fungus agent comprises the steps to prepare:
Bacterium mixes the preparation of spore powder:
With subtilis and Bacillus licheniformis, original strain carries out successively respectively under aseptic condition that slant culture, shaking table are cultivated, after the fermentor cultivation, with the fermented liquid that obtains by etc. weight mix, being prepared into bacterium through concentrate drying mixes spore powder;
Fungi mixes the preparation of spore powder:
With trichoderma harziarum, aspergillus niger, aspergillus niger, original strain carries out respectively slant culture, shaking table cultivation, fermentor cultivation, solid fermentation product spore successively under aseptic condition after, the substratum that produces spore fully that obtains is immersed in respectively makes spore suspension in the clear water, thereby obtain trichoderma harziarum spore suspension, aspergillus niger spore suspension, aspergillus oryzae spore suspension, then with the trichoderma harziarum spore suspension that obtains, aspergillus niger spore suspension, aspergillus oryzae spore suspension respectively concentrate drying be prepared into the fungal spore powder; Then, be hybridly prepared into fungi according to 1: 1: 1 ratio and mix spore powder;
The above-mentioned bacterium mixing spore powder that makes and fungi mixing spore powder are mixed according to 4: 1 ratio, thereby prepare composite fungus agent.
Particularly, described composite fungus agent comprises the steps to prepare:
Bacterium mixes the preparation of spore powder:
1) slant culture: with subtilis and Bacillus licheniformis, original strain is inoculated in respectively under aseptic condition on the slant medium, cultivates 36-48 hour under 28 ± 2 ℃ of conditions;
2) bacterial classification of shaking table cultivation: with above-mentioned steps 1) cultivating is inoculated in respectively liquid nutrient medium under aseptic condition, be under 30 ℃ of conditions in pH6.5-7.0, temperature, and the 140-160r/min shaking table was cultivated 36-48 hour;
3) fermentor cultivation: the bacterial classification that above-mentioned steps 2 is cultivated is inoculated in respectively fermentation tank culture medium under aseptic condition, be 30 ℃, ventilation 1 in pH7.5-8.0, tank pressure 0.5kg, temperature: under the 0.8-1.1 condition, cultivate after 48-56 hour, the bacterium number is greater than 1.0 * 10
10/ mL, 80% thalline change at present tank of gemma, obtain fermented liquid;
4) with the fermented liquid that obtains in the step 3 by etc. weight mix, being prepared into bacterium through concentrate drying mixes spore powder;
Mix in the preparation of spore powder step 1 on above-mentioned bacterium) in the prescription of the slant medium selected as follows: glucose 15g, fish peptone 5g, yeast extract paste 5g, water 1000mL, agar 15g.
Above-mentioned steps 2) the liquid culture based formulas in is as follows: glucose 10g, extractum carnis 5g, yeast powder 5g, starch 10g, soybean cake powder 5g, K
2HPO
40.5g, MgSO
40.2g, water 1000mL.
Above-mentioned steps 3) the fermentor cultivation based formulas in is as follows: Semen Maydis powder 26kg, soybean cake powder 16kg, ammonium sulfate 4kg, glucose 8kg, yeast powder 2.5kg, peptone 1.7kg, add water to 600kg.
Fungi mixes the preparation of spore powder:
1) with trichoderma harziarum, aspergillus niger, aspergillus niger, original strain is inoculated in respectively under aseptic condition on the slant medium, cultivates 48 hours under 28 ± 2 ℃ of conditions;
2) bacterial classification of shaking table cultivation: with above-mentioned steps 1) cultivating is inoculated in respectively liquid nutrient medium under aseptic condition, be under 30 ℃ of conditions in pH6.5-6.8, temperature, and the 160-200r/min shaking table was cultivated 24 hours;
3) fermentor cultivation: the bacterial classification that above-mentioned steps 2 is cultivated is inoculated in respectively fermentation tank culture medium under aseptic condition, be that 27-28 ℃, ventilation are 1 in pH6.5-6.8, tank pressure 0.5kg, temperature: 0.6-0.8, after cultivating 48h, mycelium account for cumulative volume 20% the time stop fermentation, carry out solid fermentation and produce spore;
4) solid fermentation produces spore: the mycelium after tank is cultivated by fermentation with above-mentioned steps 3 is inoculated on the solid fermentation substratum, cultivates 48h, and 90% produces spore;
5) substratum that produces spore in the above-mentioned steps 4 fully is immersed in respectively makes spore suspension in the clear water, thereby obtain trichoderma harziarum spore suspension, aspergillus niger spore suspension, aspergillus niger spore suspension;
6) with the trichoderma harziarum spore suspension that obtains in the above-mentioned steps 5, aspergillus niger spore suspension, aspergillus oryzae spore suspension respectively concentrate drying be prepared into fungi and mix spore powder; Then, be hybridly prepared into fungi according to 1: 1: 1 ratio and mix spore powder.
Mix in the preparation of spore powder step 1 above-mentioned fungi) in the prescription of the slant medium selected as follows: glucose 20g, murphy juice 200g, agar 20g, water 1000mL, pH nature.
Above-mentioned steps 2) the liquid culture based formulas in is as follows: white sugar 20g, yeast extract paste 0.5g.Starch 20g, potassium primary phosphate 0.5g, sal epsom 0.2g, sodium-chlor 0.2g, water 1000mL.
Above-mentioned steps 3) the fermentor cultivation based formulas in is as follows: starch 12kg, soybean cake powder 1.2kg, Semen Maydis powder 3kg, white sugar 12kg, yeast extract paste 0.3kg, sal epsom 0.12kg, sodium-chlor 0.12kg, add water to 600kg.
Above-mentioned steps 4) the solid fermentation culture medium prescription in is as follows:
Solid material: cotton seed hulls 25%, Semen Maydis powder 10%, wheat bran 65%.The weight ratio of solid material and water is 1: 0.8.
The above-mentioned bacterium mixing spore powder that makes and fungi mixing spore powder are mixed according to 4: 1 ratio, thereby prepare composite fungus agent.
Described emulsifying agent is the plant-sourced tensio-active agent, and described plant-sourced tensio-active agent is the Oil-tea-cake flooding extract.Its main component is saponins compound.
Described nutritive ingredient is iron, boron, zinc, copper, manganese and the molybdenum of white sugar, brown sugar (Saccharum Sinensis Roxb.), starch, protein powder, usefulness amino acid and/or humic acid chelating.
Described nutritive ingredient is to prepare by the following method:
With iron, boron, zinc, copper, manganese and the molybdenum of white sugar, brown sugar (Saccharum Sinensis Roxb.), starch, protein powder, usefulness amino acid and/or humic acid chelating, according to 1: 2.8: 5: 1: 0.2 quality proportioning mixed and makes.
Described soluble carrier is Zulkovsky starch or wilkinite.
The present invention also provides a kind of preparation method who prepares above-mentioned soluble straw decomposing agent, its.
It is that 10-20 part semen multiplex powder, 20-50 part nutritive ingredient, 2-5 part plant source wedding agent, 25-68 part soluble carrier mixing and stirring according to the weight part meter are prepared.
Preferably, it is that 12-18 part semen multiplex powder, 22-45 part nutritive ingredient, 2-4 part plant source wedding agent, 33-64 part soluble carrier mixing and stirring according to the weight part meter are prepared.
More preferably, it is to prepare according to 15 parts of semen multiplex powder of weight part meter, 25 parts of nutritive ingredients, 3 parts of plant source wedding agents, 57 parts of soluble carrier mixing and stirring.
The present invention also provides the application of above-mentioned soluble straw decomposing agent, its in crops straw returning field.
This soluble straw decomposing agent, its can carry out spray application by atomizer in field or stalk composting, described soluble straw decomposing agent, its can spray according to 200-300 rear the using with land for growing field crops mechanize by atomizer spray of scale water dilution doubly, also can spread fertilizer over the fields by the method for pulvis.
Described soluble straw decomposing agent, its can accelerate the degradation speed of multiple kinds of crops stalk and promote crop growth and prevention multiple kinds of crops disease and pest.
All available from China Committee for Culture Collection of Microorganisms agricultural microorganism center, the preserving number of five bacterial classifications is as described below respectively for five bacterial classifications that the present invention selects::
1) trichoderma harziarum (Trichoderma harzianum) China Committee for Culture Collection of Microorganisms agricultural microorganism center ACCC 31707;
2) aspergillus niger (Aspergillus niger) China Committee for Culture Collection of Microorganisms agricultural microorganism center ACCC 30797;
3) aspergillus oryzae (Aspergillus oryzae) China Committee for Culture Collection of Microorganisms agricultural microorganism center ACCC 30788;
4) subtilis (Bacillus subtilis) China Committee for Culture Collection of Microorganisms agricultural microorganism center ACCC10118;
5) bacillus licheniformis (Bacillus licheniformis) China Committee for Culture Collection of Microorganisms agricultural microorganism center ACCC10618.
Advantage of the present invention is:
1, microbe species collocation of the present invention is reasonable, and wood is mould and the cellulase system aspergillus combination results is with strong complementarity.Although Bacillus licheniformis can not be used as the cellulose-decomposing bacteria use because not producing cellulase, it can produce multiple protein enzyme and antagonistic substance and phytokinin and dormin etc.After it and subtilis and Trichodermaharzianum Rifai collocation, can strengthen the ability of protease activity and the antagonism pathogen of crop of product, play the biological and ecological methods to prevent plant disease, pests, and erosion growth-promoting functions.
2, the advanced science of effective bacterium preparation method of the present invention, simple and feasible, spore count content is high, and the survival time is long.Add plant source wedding agent and can make the good dispersity of spore in water, the tackiness on stalk is strong, adds nutritive ingredient spore is grown on stalk fast surely.
3, soluble straw decomposing agent, its of the present invention is that powder dose-type again can be entirely water-soluble.Application process is simple and practical, can spread fertilizer over the fields, and can according to spraying after the 200-300 scale water dilution doubly, be applicable to mechanized again.
4, the field effect of soluble straw decomposing agent, its of the present invention is good: this product is through at the agricultural crop straw stacking decomposition such as paddy rice, corn, rape and application test on the field also, the result shows: the time of using the straw stacks temperature of this product to reach top temperature 65 degree will shift to an earlier date 8 to 10 days than contrast, stacking height drop to former height half namely time of straw decomposing than contrast in advance 15 to 20.Do base manure kind garlic and cabbage heart with the stalk of this soluble straw decomposing agent, its decomposition, the result shows: can significantly promote growing of garlic and cabbage heart, garlic on average increases production 6.9%, and cabbage heart on average increases production 10%; The sickness rate of garlic and cabbage heart reduces 70%.
Embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is described in further detail.Following examples are used for explanation the present invention, but are not used for limiting the scope of the invention.
The formation of embodiment 1, soluble straw decomposing agent, its
Composite fungus agent 10 weight parts;
Nutritive ingredient 20 weight parts;
Plant-sourced emulsification permeate agent 2 weight parts;
Soluble carrier 68 weight parts.
The formation of described composite fungus agent
Trichoderma harziarum 0.2 hundred million cfu/g;
Aspergillus niger 0.5 hundred million cfu/g;
Aspergillus oryzae 0.5 hundred million cfu/g;
Subtilis 1.0 hundred million cfu/g;
Bacillus licheniformis 1.0 hundred million cfu/g;
Effective bacterium total content 〉=300,000,000 cfu/g.
The microbial inoculum of above-mentioned formation is made by following steps:
Bacterium mixes the preparation of spore powder:
1) slant culture: with subtilis and Bacillus licheniformis, original strain is inoculated in respectively under aseptic condition on the slant medium, cultivates 48 hours under 28 ± 2 ℃ of conditions;
2) bacterial classification of shaking table cultivation: with above-mentioned steps 1) cultivating is inoculated in respectively liquid nutrient medium under aseptic condition, be under 30 ℃ of conditions in pH6.5, temperature, and the 160r/min shaking table was cultivated 36 hours;
3) fermentor cultivation: the bacterial classification that above-mentioned steps 2 is cultivated is inoculated in respectively fermentation tank culture medium under aseptic condition, be 30 ℃, ventilation 1 in pH8.0, tank pressure 0.5kg, temperature: under the 0.8-1.1 condition, cultivate after 56 hours, the bacterium number is greater than 1.0 * 10
10/ mL, 80% thalline change at present tank of gemma, obtain fermented liquid;
4) with the fermented liquid that obtains in the step 3 by etc. weight mix, being prepared into bacterium through concentrate drying mixes spore powder;
Mix in the preparation of spore powder step 1 on above-mentioned bacterium) in the prescription of the slant medium selected as follows: glucose 15g, fish peptone 5g, yeast extract paste 5g, water 1000mL, agar 15g.
Above-mentioned steps 2) the liquid culture based formulas in is as follows: glucose 10g, extractum carnis 5g, yeast powder 5g, starch 10g, soybean cake powder 5g, K
2HPO
40.5g, MgSO
40.2g, water 1000mL.
Above-mentioned steps 3) the fermentor cultivation based formulas in is as follows: Semen Maydis powder 26kg, soybean cake powder 16kg, ammonium sulfate 4kg, glucose 8kg, yeast powder 2.5kg, peptone 1.7kg, add water to 600kg.
Fungi mixes the preparation of spore powder:
1) with trichoderma harziarum, aspergillus niger, aspergillus niger, original strain is inoculated in respectively under aseptic condition on the slant medium, cultivates 48 hours under 28 ± 2 ℃ of conditions;
2) bacterial classification of shaking table cultivation: with above-mentioned steps 1) cultivating is inoculated in respectively liquid nutrient medium under aseptic condition, be under 30 ℃ of conditions in pH6.6, temperature, and the 160r/min shaking table was cultivated 24 hours;
3) fermentor cultivation: the bacterial classification that above-mentioned steps 2 is cultivated is inoculated in respectively fermentation tank culture medium under aseptic condition, be that 28 ℃, ventilation are 1: 0.6 in pH6.6, tank pressure 0.5kg, temperature, after cultivating 48h, mycelium account for cumulative volume 20% the time stop fermentation, carry out solid fermentation and produce spore;
4) solid fermentation produces spore: the mycelium after tank is cultivated by fermentation with above-mentioned steps 3 is inoculated on the solid fermentation substratum, cultivates 48h, and 90% produces spore;
5) substratum that produces spore in the above-mentioned steps 4 fully is immersed in respectively makes spore suspension in the clear water, thereby obtain trichoderma harziarum spore suspension, aspergillus niger spore suspension, aspergillus niger spore suspension;
6) with the trichoderma harziarum spore suspension that obtains in the above-mentioned steps 5, aspergillus niger spore suspension, aspergillus oryzae spore suspension respectively concentrate drying be prepared into fungi and mix spore powder; Then, be hybridly prepared into fungi according to 1: 1: 1 ratio and mix spore powder.
Mix in the preparation of spore powder step 1 above-mentioned fungi) in the prescription of the slant medium selected as follows: glucose 20g, murphy juice 200g, agar 20g, water 1000mL, pH nature.
Above-mentioned steps 2) the liquid culture based formulas in is as follows: white sugar 20g, yeast extract paste 0.5g.Starch 20g, potassium primary phosphate 0.5g, sal epsom 0.2g, sodium-chlor 0.2g, water 1000mL.
Above-mentioned steps 3) the fermentor cultivation based formulas in is as follows: starch 12kg, soybean cake powder 1.2kg, Semen Maydis powder 3kg, white sugar 12kg, yeast extract paste 0.3kg, sal epsom 0.12kg, sodium-chlor 0.12kg, add water to 600kg.
Above-mentioned steps 4) the solid fermentation culture medium prescription in is as follows:
Solid material: cotton seed hulls 25kg, Semen Maydis powder 10kg, wheat bran 65kg.The weight ratio of solid material and water is 1: 0.8.
The above-mentioned bacterium mixing spore powder that makes and fungi mixing spore powder are mixed according to 4: 1 ratio, thereby prepare composite fungus agent.
Described emulsifying agent is the plant-sourced tensio-active agent, and described plant-sourced tensio-active agent is the Oil-tea-cake extractive substance.
Described nutritive ingredient is to prepare by the following method:
With iron, boron, zinc, copper, manganese and the molybdenum of white sugar, brown sugar (Saccharum Sinensis Roxb.), starch, protein powder, usefulness amino acid and/or humic acid chelating, according to 1: 2.8: 5: 1: 0.2 quality proportioning mixed and makes.
Described iron, boron, zinc, copper, manganese and the molybdenum of amino acid and/or humic acid chelating used is available from Chengdu chelating Bioisystech Co., Ltd.Name of product is chelated microelement.Wherein iron, boron, zinc, copper, manganese, molybdenum are amino-acid chelate.Chelated microelements total content (iron+boron+zinc+copper+manganese+molybdenum) 〉=8%.
Described soluble carrier is Zulkovsky starch.
The formation of embodiment 2, soluble straw decomposing agent, its
Composite fungus agent 15 weight parts;
Nutritive ingredient 25 weight parts;
Plant-sourced emulsification permeate agent 3 weight parts;
Soluble carrier 57 weight parts.
The formation of described composite fungus agent
Trichoderma harziarum 0.5 hundred million cfu/g;
Aspergillus niger 1.0 hundred million cfu/g;
Aspergillus oryzae 1.0 hundred million cfu/g;
Subtilis 5.0 hundred million cfu/g;
Bacillus licheniformis 5.0 hundred million cfu/g;
Effective bacterium total content 〉=1,200,000,000 cfu/g.
The used substratum of the used substratum of each step and embodiment 1 corresponding steps was identical when the preparation bacterium mixed spore powder and preparation fungi mixing spore powder in above-described embodiment 2, and the compound method of product is identical with ratio.Described nutritive ingredient, plant-sourced emulsification permeate agent are with embodiment 1.Described soluble carrier is wilkinite.
Described iron, boron, zinc, copper, manganese and the molybdenum of amino acid and/or humic acid chelating used is available from Chengdu chelating Bioisystech Co., Ltd.Name of product is chelated microelement.Wherein iron, boron, zinc, copper are amino-acid chelate; Manganese, molybdenum are the humic acid chelating thing.Chelated microelements total content (iron+boron+zinc+copper+manganese+molybdenum) 〉=8%.
The formation of embodiment 3, soluble straw decomposing agent, its
Composite fungus agent 20 weight parts;
Nutritive ingredient 50 weight parts;
Plant-sourced emulsification permeate agent 5 weight parts;
Soluble carrier 25 weight parts.
The formation of described composite fungus agent
Trichoderma harziarum 1.05 hundred million cfu/g;
Aspergillus niger 2.0 hundred million cfu/g;
Aspergillus oryzae 2.0 hundred million cfu/g;
Subtilis 5.0 hundred million cfu/g;
Bacillus licheniformis 5.0 hundred million cfu/g;
Effective bacterium total content 〉=1,500,000,000 cfu/g.
The used substratum of the used substratum of each step and embodiment 1 corresponding steps was identical when the preparation bacterium mixed spore powder and preparation fungi mixing spore powder in above-described embodiment 3, and the compound method of product is identical with ratio.Described nutritive ingredient, plant-sourced emulsification permeate agent are with embodiment 1.Described soluble carrier is wilkinite.
Described iron, boron, zinc, copper, manganese and the molybdenum of amino acid and/or humic acid chelating used is with embodiment 1.
The formation of embodiment 4 soluble straw decomposing agent, its
Composite fungus agent 12 weight parts;
Nutritive ingredient 22 weight parts;
Plant-sourced emulsification permeate agent 2 weight parts;
Soluble carrier 64 weight parts.
The formation of described composite fungus agent
Trichoderma harziarum 0.2 hundred million cfu/g;
Aspergillus niger 0.5 hundred million cfu/g;
Aspergillus oryzae 0.5 hundred million cfu/g;
Subtilis 1.0 hundred million cfu/g;
Bacillus licheniformis 1.0 hundred million cfu/g;
Effective bacterium total content 〉=300,000,000 cfu/g.
The used substratum of the used substratum of each step and embodiment 1 corresponding steps was identical when the preparation bacterium mixed spore powder and preparation fungi mixing spore powder in above-described embodiment 4, and the compound method of product is identical with ratio.Described nutritive ingredient, plant-sourced emulsification permeate agent are with embodiment 1.Described soluble carrier is Zulkovsky starch.
Described iron, boron, zinc, copper, manganese and the molybdenum of amino acid and/or humic acid chelating used is with embodiment 2.
The formation of embodiment 5 soluble straw decomposing agent, its
Composite fungus agent 18 weight parts;
Nutritive ingredient 45 weight parts;
Plant-sourced emulsification permeate agent 4 weight parts;
Soluble carrier 33 weight parts.
The formation of described composite fungus agent
Trichoderma harziarum 1.05 hundred million cfu/g;
Aspergillus niger 2.0 hundred million cfu/g;
Aspergillus oryzae 2.0 hundred million cfu/g;
Subtilis 5.0 hundred million cfu/g;
Bacillus licheniformis 5.0 hundred million cfu/g;
Effective bacterium total content 〉=1,500,000,000 cfu/g.
The used substratum of the used substratum of each step and embodiment 1 corresponding steps was identical when the preparation bacterium mixed spore powder and preparation fungi mixing spore powder in above-described embodiment 5, and the compound method of product is identical with ratio.Described nutritive ingredient, plant-sourced emulsification permeate agent are with embodiment 1.Described soluble carrier is Zulkovsky starch.
Described iron, boron, zinc, copper, manganese and the molybdenum of amino acid and/or humic acid chelating used is available from Chengdu chelating Bioisystech Co., Ltd.Name of product is chelated microelement.Wherein iron, boron, zinc, copper, manganese, molybdenum are the humic acid chelating thing.Chelated microelements total content (iron+boron+zinc+copper+manganese+molybdenum) 〉=8%.
Water-soluble and the spray effect test of experimental example 1, a kind of soluble straw decomposing agent, its
1, test objective:
Water-soluble and the spray effect of checking soluble straw decomposing agent, its.
2, test materials:
The soluble straw decomposing agent, its that is prepared by embodiment 2: pale yellow powder, living bacteria count 〉=1,200,000,000/gram.
Atomizer: knapsack hand sprayer (Kai Tai, model: 3WBS-16).
Urea: general application urea.
Water: the spraying water is tap water (water temperature was 10 ℃ at that time).
Test method:
3, test arranges two processing:
Process 1: by recommendation ratio soluble straw decomposing agent, its: water: urea=150: 45000: 4000 (weight ratio) preparation 15L suspension, with also timing of common sprayer spraying, observe water-soluble and spray effect.
Process 2 (contrasts): do not add soluble straw decomposing agent, its, (water: urea :=45000: 4000) (weight ratio) preparation 15L solution is observed spray effect in contrast with common sprayer spraying and timing in the recommendation ratio.
Observe respectively the spray velocity that each is processed, namely under the same pressure, sprayed the used time of same solution; Each spray effect of processing of contrast.
4, test-results:
1) water-soluble: as soluble straw decomposing agent, its to be added in the urea soln that configures, stirred 30 seconds, namely form uniform suspension, without the lumps material, illustrate that soluble straw decomposing agent, its is water-soluble good.
2) spray time:
In the lower spraying of same pressure (0.2-0.3MPa), process 1 and sprayed 21 minutes and 45 seconds 15L suspension time spent; Process 2 and sprayed 20 minutes and 56 seconds 15L solution time spent.
3) spray effect:
In the spray process, process 1 spraying smooth and easy, even, the unusual phenomenoies such as obstruction do not occur; Spray effect is similar with processing 2; After the microbial inoculum suspension had all sprayed, the pulvis precipitation was not left at the bucket end.
5, conclusion:
A kind of soluble straw decomposing agent, its, in the thin up situation, water-soluble good, spray effect is good, can use with the mode of spraying.
Experimental example 2, a kind of water-soluble and spray effect test that does not add the soluble straw decomposing agent, its of emulsifying agent
1, test objective:
Water-soluble and the spray effect of checking soluble straw decomposing agent, its (not adding emulsifying agent).
2, test materials:
2.1 the soluble straw decomposing agent, its that is prepared by embodiment 2: pale yellow powder, living bacteria count 〉=12. hundred million/gram.
2.2 do not add the soluble straw decomposing agent, its (10 parts of composite fungus agents, 20 parts of nutritions, 68 parts of soluble carrier) of emulsifying agent: pale yellow powder, living bacteria count 〉=12.0 hundred million/gram.
2.3 atomizer: knapsack hand sprayer (Kai Tai, model: 3WBS-16).
2.4 urea: general application urea.
2.5 water: the spraying water is tap water (water temperature was 10 ℃ at that time).
Test method:
3, test arranges three processing:
Process 1: by recommendation ratio soluble straw decomposing agent, its (embodiment 2 makes): water: urea=150: 45000: 4000 (weight ratio) preparation 15L suspension, with also timing of common sprayer spraying, observe water-soluble and spray effect.
Process 2: the soluble straw decomposing agent, its that does not add emulsifying agent in the recommendation ratio: water: urea=150: 45000: 4000 (weight ratio) preparation 15L suspension, with common sprayer spraying and timing, observe water-soluble and spray effect.
Process 3 (contrasts): do not add soluble straw decomposing agent, its, (water: urea :=45000: 4000) (weight ratio) preparation 15L solution is observed spray effect in contrast with common sprayer spraying and timing in the recommendation ratio.
Observe respectively the spray velocity that each is processed, namely under the same pressure, sprayed the used time of same solution; Each spray effect of processing of contrast.
4, test-results:
1) water-soluble: processing 1 adds soluble straw decomposing agent, its in the urea soln that configures, and stirs 30 seconds, namely forms uniform suspension, without the lumps material, illustrates that soluble straw decomposing agent, its is water-soluble good.Processing 2 soluble straw decomposing agent, its that will not add emulsifying agent adds in the urea soln that configures, stirred 30 seconds, namely form muddy suspension, a little thicker suspended substance is arranged, illustrate and process 1 soluble straw decomposing agent, its that adds emulsifying agent does not add the soluble straw decomposing agent, its of emulsifying agent than processing 2 good water solubility
2) spray time:
In the lower spraying of same pressure (0.2-0.3MPa), process 1 and sprayed 21 minutes and 45 seconds 15L suspension time spent; Process 2 and sprayed 35 minutes and 36 seconds 15L solution time spent; Process 3 and sprayed 20 minutes and 56 seconds 15L solution time spent.
3) spray effect:
In the spray process, process 1 spraying smooth and easy, even, the unusual phenomenoies such as obstruction do not occur; Spray effect is similar with processing 3; After the microbial inoculum suspension had all sprayed, the pulvis precipitation was not left at the bucket end.Process 2 sprayings not bery smooth and easy, a little latch up phenomenon is arranged, after the microbial inoculum suspension had sprayed, a little pulvis precipitation was left at the bucket end.
5, conclusion:
A kind of soluble straw decomposing agent, its, in the thin up situation, water-soluble good, spray effect is good, can use with the mode of spraying; The soluble straw decomposing agent, its that does not add emulsifying agent can be used with the mode of spraying, but poor than the soluble straw decomposing agent, its that adds emulsifying agent of water-soluble and spray effect.
The degrading maize straws effect test of experimental example 3, a kind of soluble straw decomposing agent, its reaches the impact experiment on vegetable growth
1, test objective:
Verify a kind of straw degradative effect of soluble straw decomposing agent, its and on the impact of vegetable growth.
2, test materials:
The soluble straw decomposing agent, its that is prepared by embodiment 2: pale yellow powder, living bacteria count 〉=1,200,000,000/gram.
Stalk: maize straw (pulverizing)
Urea: general application urea
Water: tap water
For trying vegetables: Plantula Brassicae chinensis
3, test design:
1) test site: greenhouse
Room temp control: 22 ℃-25 ℃
Medial humidity: 60%
2) the straw degradative test arranges 3 processing:
Process 1: stalk (220g)+a kind of soluble straw decomposing agent, its+urea+water
Process 2: stalk (220g)+urea+water
Process 3: stalk (220g)+water
3) the impact test of vegetable growth arranged 3 processing, each processes 3 repetitions:
Process I: stalk (50g)+a kind of soluble straw decomposing agent, its+urea+water
Process II: stalk (50g)+urea+water
Process III: do not add stalk
4, test method:
1) straw degradative test:
(1) prepare bacterium liquid or solution:
Process 1: in recommendation ratio (a kind of soluble straw decomposing agent, its: water: urea=150: 45000: 4000) (weight ratio) preparation bacterium liquid.
Process 2: do not add a kind of soluble straw decomposing agent, its, in recommendation ratio (water: urea=45000: 4000) (weight ratio) obtain solution.
Process 3: only add water.
(2) maize straw is processed 220g dress basin by each, calculate by the recommendation amount, process 1 and spray bacterium liquid 16.5ml, process 2 and spray urea soln 16.5ml, process 3 and spray water 16.5ml, with soil stalk is sealed.
(3) spray water every day, keep stalk humidity, be beneficial to microorganism growth and straw decomposing, each Treating straw injection flow rate is basically identical, and keeping water content is 60%.
(4) observed a straw decomposition degree in per three days, project comprises: color, feel, stretching resistance.
(5) with each Treating straw oven dry, take by weighing quality, calculate the decomposition rate, relatively the decomposition speed of each processing.
2) impact of vegetable growth is tested
(1) prepare bacterium liquid or solution:
Process I: in recommendation ratio (a kind of soluble straw decomposing agent, its: water: urea=150: 45000: 4000) (weight ratio) preparation bacterium liquid.
Process II: do not add a kind of soluble straw decomposing agent, its, in recommendation ratio (water: urea=45000: 4000) (weight ratio) obtain solution.
Process III: only add water.
(2) every basin fills native 5kg, and maize straw is processed 50g by each, calculates by the recommendation amount, processes I and sprays bacterium liquid 3.75ml, processes II and sprays urea soln 3.75ml, processes III and does not add stalk, maize straw is packed into even with mixing with soil in the basin.
(3) spray water every day, keep stalk humidity, be beneficial to microorganism growth and straw decomposing, each Treating straw injection flow rate is basically identical.
After (4) 15 days, the plantation Plantula Brassicae chinensis, seedling 6 strains are stayed in the rear every processing of emerging, and guarantee that agronomic measures (implantation time, crop varieties, rate of fertilizer application, irrigation quantity, pesticide dosage) is consistent between each processing.
(5) during Growth of Cabbage, measured respectively width, length, petiole length and the number of blade of its blade at the 10th, 20,30 day; When results, all gather in, weigh, calculate the output of Plantula Brassicae chinensis.
2, test materials:
The soluble straw decomposing agent, its that is prepared by embodiment 2: pale yellow powder, living bacteria count 〉=1,200,000,000/gram.
Stalk: maize straw (pulverizing)
Urea: general application urea
Water: tap water
For trying vegetables: Plantula Brassicae chinensis
3, test design:
1) test site: greenhouse
Room temp control: 22 ℃-25 ℃
Medial humidity: 60%
2) the straw degradative test arranges 3 processing:
Process 1: stalk (220g)+a kind of soluble straw decomposing agent, its+urea+water
Process 2: stalk (220g)+urea+water
Process 3: stalk (220g)+water
3) the impact test of vegetable growth arranged 3 processing, each processes 3 repetitions:
Process I: stalk (50g)+a kind of soluble straw decomposing agent, its+urea+water
Process II: stalk (50g)+urea+water
Process III: do not add stalk
4, test method:
1) straw degradative test:
(1) prepare bacterium liquid or solution:
Process 1: in recommendation ratio (a kind of soluble straw decomposing agent, its: water: urea=150: 45000: 4000) (weight ratio) preparation bacterium liquid.
Process 2: do not add a kind of soluble straw decomposing agent, its, in recommendation ratio (water: urea=45000: 4000) (weight ratio) obtain solution.
Process 3: only add water.
(2) maize straw is processed 220g dress basin by each, calculate by the recommendation amount, process 1 and spray bacterium liquid 16.5ml, process 2 and spray urea soln 16.5ml, process 3 and spray water 16.5ml, with soil stalk is sealed.
(3) spray water every day, keep stalk humidity, be beneficial to microorganism growth and straw decomposing, each Treating straw injection flow rate is basically identical, and keeping water content is 60%.
(4) observed a straw decomposition degree in per three days, project comprises: color, feel, stretching resistance.
(5) with each Treating straw oven dry, take by weighing quality, calculate the decomposition rate, relatively the decomposition speed of each processing.
2) impact of vegetable growth is tested
(1) prepare bacterium liquid or solution:
Process I: in recommendation ratio (a kind of soluble straw decomposing agent, its: water: urea=150: 45000: 4000) preparation bacterium liquid.
Process II: do not add a kind of soluble straw decomposing agent, its, in recommendation ratio (water: urea=45000: 4000) obtain solution.
Process III: only add water.
(2) every basin fills native 5kg, and maize straw is processed 50g by each, calculates by the recommendation amount, processes I and sprays bacterium liquid 3.75ml, processes II and sprays urea soln 3.75ml, processes III and does not add stalk, maize straw is packed into even with mixing with soil in the basin.
(3) spray water every day, keep stalk humidity, be beneficial to microorganism growth and straw decomposing, each Treating straw injection flow rate is basically identical.
After (4) 15 days, the plantation Plantula Brassicae chinensis, seedling 6 strains are stayed in the rear every processing of emerging, and guarantee that agronomic measures (implantation time, crop varieties, rate of fertilizer application, irrigation quantity, pesticide dosage) is consistent between each processing.
(5) during Growth of Cabbage, measured respectively width, length, petiole length and the number of blade of its blade at the 10th, 20,30 day; When results, all gather in, weigh, calculate the output of Plantula Brassicae chinensis.
5, test-results:
1) color, feel and stretching resistance change:
The straw decomposition changing conditions that compares 3 processing from color, feel and stretching resistance aspect, the straw decomposition effect that two weeks processed by a kind of soluble straw decomposing agent, its is remarkable, 1 the decomposition speed processed processes 2 and process 3 soon, checks through on-the-spot naked eyes and to find that 1 degree of becoming thoroughly decomposed of processing processes 2 and process 3 large.
2) decomposition rate:
Through calculating, aftertreatment in 15 days 1 decomposition rate is 90.5%, exceeds 52.5% than processing 2 (the decomposition rate is 38.0%), exceeds 56.4% than processing 3 (the decomposition rate is 34.1%).Illustrate and use a kind of soluble straw decomposing agent, its can significantly accelerate maize straw decomposition speed.
3) different treatment is on the impact of Plantula Brassicae chinensis economic characters and output:
Can find out from test, use a kind of processing I of soluble straw decomposing agent, its wide to plant height, the leaf of Plantula Brassicae chinensis, leaf is long and the number of blade has obvious promoter action, process I and compare with the processing II that does not use a kind of soluble straw decomposing agent, its, plant height, leaf is wide, leaf is long and the number of blade on average increases by 2.3 centimetres, 0.5 centimetre, 0.7 centimetre and 1; Compare plant height with the processing III without stalk, leaf is wide, leaf is long and the number of blade on average increases by 3.0 centimetres, 0.7 centimetre, 1.2 centimetres and 1.2.Remarkable to the Growth of Cabbage facilitation effect.
Can find out from test: use a kind of processing I of soluble straw decomposing agent, its, yield of pakchoi increases obviously, processes I and compares with the processing II that does not use a kind of soluble straw decomposing agent, its, and yield of pakchoi increases by 10.2%; Compare with the processing III without stalk, yield of pakchoi increases by 12.3%.Obvious effect of increasing production.
6, conclusion:
After a kind of soluble straw decomposing agent, its is processed, maize straw color generation considerable change, feel and stretching resistance change all remarkable than control group; As calculated, the test group decomposition rate of processing through a kind of soluble straw decomposing agent, its is 90.5%, and than processing 2 and process 3 and exceed 52.5% and 56.4%, decomposition speed is obviously faster than control group respectively.
Pot experiment proves, can significantly promote growing of Plantula Brassicae chinensis behind the maize straw after a kind of soluble straw decomposing agent, its is processed, and can significantly improve the output of Plantula Brassicae chinensis.With need not compare by a kind of soluble straw decomposing agent, its, the output of Plantula Brassicae chinensis increases by 10.2%; With do not have straw-returning to compare, yield of pakchoi increases by 12.3%.
The degrading maize straws effect test of the soluble straw decomposing agent, its of experimental example 4, a kind of not Ensure Liquid composition reaches the impact experiment on vegetable growth
1, test objective:
Verify a kind of not Ensure Liquid composition soluble straw decomposing agent, its straw degradative effect and on the impact of vegetable growth.
2, test materials:
2.1 the soluble straw decomposing agent, its that is prepared by embodiment 2: pale yellow powder, living bacteria count 〉=1,200,000,000/gram.
2.2 soluble straw decomposing agent, its of Ensure Liquid composition (10 parts of composite fungus agents, 2 parts of plant-sourced emulsification permeate agents, 88 parts of soluble carrier) not: pale yellow powder, living bacteria count 〉=1,200,000,000/gram.
2.3 stalk: maize straw (pulverizing)
2.4 urea: general application urea
2.5 water: tap water
2.6 for trying vegetables: Plantula Brassicae chinensis
3, test design:
1) test site: greenhouse.
Room temp control: 22 ℃-25 ℃
Medial humidity: 60%
2) the straw degradative test arranges 4 processing:
Process 1: stalk (220g)+a kind of soluble straw decomposing agent, its+urea+water
Process 2: the soluble straw decomposing agent, its+urea of stalk (220g)+a kind of not Ensure Liquid composition+water
Process 3: stalk (220g)+urea+water
Process 4: stalk (220g)+water
3) the impact test of vegetable growth arranged 4 processing, each processes 3 repetitions:
Process I: stalk (50g)+a kind of soluble straw decomposing agent, its+urea+water
Process II: the soluble straw decomposing agent, its+urea of stalk (50g)+a kind of not Ensure Liquid composition+water
Process III: stalk (50g)+urea+water
Process IV: do not add stalk
4, test method:
1) straw degradative test:
(1) prepare bacterium liquid or solution:
Process 1: in recommendation ratio (a kind of soluble straw decomposing agent, its: water: urea=150: 45000: 4000) (weight ratio) preparation bacterium liquid.
Process 2: in recommendation ratio (a kind of soluble straw decomposing agent, its of not Ensure Liquid composition: water: urea=150: 45000: 4000) preparation bacterium liquid.
Process 3: do not add a kind of soluble straw decomposing agent, its, in recommendation ratio (water: urea=45000: 4000) (weight ratio) obtain solution.
Process 4: only add water.
(2) maize straw is processed 220g dress basin by each, calculate by the recommendation amount, process 1 and spray bacterium liquid 16.5ml, process 2 and spray bacterium liquid 16.5ml, process 3 and spray urea soln 16.5ml, process 4 and spray water 16.5ml, with soil stalk is sealed.
(3) spray water every day, keep stalk humidity, be beneficial to microorganism growth and straw decomposing, each Treating straw injection flow rate is basically identical, and keeping water content is 60%.
(4) observed a straw decomposition degree in per three days, project comprises: color, feel, stretching resistance.
(5) with each Treating straw oven dry, take by weighing quality, calculate the decomposition rate, relatively the decomposition speed of each processing.
2) impact of vegetable growth is tested
(1) prepare bacterium liquid or solution:
Process I: in recommendation ratio (a kind of soluble straw decomposing agent, its: water: urea=150: 45000: 4000) (weight ratio) preparation bacterium liquid.
Process II: in recommendation ratio (a kind of soluble straw decomposing agent, its of not Ensure Liquid composition: water: urea=150: 45000: 4000) (weight ratio) preparation bacterium liquid.
Process III: do not add a kind of soluble straw decomposing agent, its, in recommendation ratio (water: urea=45000: 4000) (weight ratio) obtain solution.
Process IV: only add water.
(2) every basin fills native 5kg, and maize straw is processed 50g by each, calculates by the recommendation amount, process I and spray bacterium liquid 3.75ml, process II and spray bacterium liquid 3.75ml, process III and spray urea soln 3.75ml, process IV and do not add stalk, maize straw is packed into even with mixing with soil in the basin.
(3) spray water every day, keep stalk humidity, be beneficial to microorganism growth and straw decomposing, each Treating straw injection flow rate is basically identical.
After (4) 15 days, the plantation Plantula Brassicae chinensis, seedling 6 strains are stayed in the rear every processing of emerging, and guarantee that agronomic measures (implantation time, crop varieties, rate of fertilizer application, irrigation quantity, pesticide dosage) is consistent between each processing.
(5) during Growth of Cabbage, measured respectively width, length, petiole length and the number of blade of its blade at the 10th, 20,30 day; When results, all gather in, weigh, calculate the output of Plantula Brassicae chinensis.
5, test-results:
1) color, feel and stretching resistance change:
The straw decomposition changing conditions that compares 4 processing from color, feel and stretching resistance aspect, the straw decomposition effect that two weeks processed by a kind of soluble straw decomposing agent, its is remarkable, 1 the decomposition speed processed processes 2, process 3 and process 4 soon, checks through on-the-spot naked eyes and to find that 1 degree of becoming thoroughly decomposed of processing processes 2, processes 3 and process 4 large.
2) decomposition rate:
Through calculating, aftertreatment in 15 days 1 decomposition rate is 90.5%, exceeds 12.5% than processing 2 (the decomposition rate is 78.0%), exceeds 52.5% than processing 3 (the decomposition rate is 38.0%), exceeds 56.4% than processing 4 (the decomposition rate is 34.1%).Illustrate and use a kind of soluble straw decomposing agent, its can significantly accelerate maize straw decomposition speed.Process 2 one kinds not the soluble straw decomposing agent, its of Ensure Liquid composition also can significantly accelerate maize straw decomposition speed, but than the soluble straw decomposing agent, its of the Ensure Liquid composition decomposition velocity contrast to stalk.
3) different treatment is on the impact of Plantula Brassicae chinensis economic characters and output:
Can find out from test, use a kind of processing I of soluble straw decomposing agent, its wide to plant height, the leaf of Plantula Brassicae chinensis, leaf is long and the number of blade has obvious promoter action, the soluble straw decomposing agent, its of processing I and a kind of not Ensure Liquid composition is processed II and is compared, and plant height, leaf is wide, leaf is long and the number of blade on average increases by 1.0 centimetres, 0.17 centimetre, 0.5 centimetre and 0.5; Do not use a kind of processing III of soluble straw decomposing agent, its to compare, plant height, leaf is wide, leaf is long and the number of blade on average increases by 2.3 centimetres, 0.5 centimetre, 0.7 centimetre and 1; Compare plant height with the processing IV without stalk, leaf is wide, leaf is long and the number of blade on average increases by 3.0 centimetres, 0.7 centimetre, 1.2 centimetres and 1.2.Remarkable to the Growth of Cabbage facilitation effect.Process one kind of II not the soluble straw of Ensure Liquid composition become thoroughly decomposed and compare with the processing III that does not use a kind of soluble straw decomposing agent, its, plant height, leaf is wide, leaf is long and the number of blade on average increases by 0.8 centimetre, 0.2 centimetre, 0.4 centimetre and 0.2; Compare plant height with the processing IV without stalk, leaf is wide, leaf is long and the number of blade on average increases by 0.9 centimetre, 0.5 centimetre, 0.8 centimetre and 0.4.Growth to Plantula Brassicae chinensis has certain facilitation effect, but effect does not have soluble straw decomposing agent, its good of Ensure Liquid composition.
Can find out from test: use a kind of processing I of soluble straw decomposing agent, its of Ensure Liquid composition, yield of pakchoi increases obviously, and the soluble straw decomposing agent, its processing II that processes I and a kind of not Ensure Liquid composition compares, and yield of pakchoi increases by 6.8%; Do not use a kind of processing III of soluble straw decomposing agent, its to compare, yield of pakchoi increases by 10.2%; Compare with the processing IV without stalk, yield of pakchoi increases by 12.3%.Obvious effect of increasing production.
6, conclusion:
After a kind of soluble straw decomposing agent, its is processed, maize straw color generation considerable change, feel and stretching resistance change all remarkable than control group; As calculated, the test group decomposition rate of processing through a kind of soluble straw decomposing agent, its is 90.5%, and than processing 2, processing 3 and process 4 and exceed 12.5%, 52.5% and 56.4%, decomposition speed is obviously faster than control group respectively.The test group decomposition rate of processing through a kind of soluble straw decomposing agent, its of not Ensure Liquid composition is 78.0%, and respectively than processing 3 and process 4 and exceed 40% and 43.9%, but the decomposition rate significantly is lower than a kind of processing 1 of soluble straw decomposing agent, its of Ensure Liquid composition.
Pot experiment proves, can significantly promote growing of Plantula Brassicae chinensis behind the maize straw after a kind of soluble straw decomposing agent, its is processed, and can significantly improve the output of Plantula Brassicae chinensis.Compare with a kind of soluble straw decomposing agent, its of not Ensure Liquid composition, yield of pakchoi increases by 6.8%; With need not compare by a kind of soluble straw decomposing agent, its, the output of Plantula Brassicae chinensis increases by 10.2%; With do not have straw-returning to compare, yield of pakchoi increases by 12.3%.
The degrading rice straw effect test of experimental example 5, a kind of soluble straw decomposing agent, its reaches the impact experiment on vegetable growth
1, test objective:
Verify a kind of straw degradative effect of soluble straw decomposing agent, its and on the impact of vegetable growth.
2, test materials:
2.1 straw decomposing inoculant: the soluble straw decomposing agent, its that is prepared by embodiment 2: pale yellow powder, living bacteria count 〉=1,200,000,000/gram.
Stalk: rice straw
Urea: general application urea
Water: tap water
For trying vegetables: four or nine cabbage hearts.
3, test design:
1) test site: experimental field be positioned at and decide ocean, mountain behind the cities and towns, the farmland covered by contract of the Mo Chaofang of peasant household.
2) design: test arranges 3 processing:
Process 1.: total straw is field+(a kind of soluble straw decomposing agent, its+urea+water)+conventional fertilizer application (base manure 120 kg/mu) also;
Process 2. CK I: total straw is field+urea+water+conventional fertilizer application (base manure 120 kg/mu) also;
Process 3. CK II: conventional fertilizer application (base manure 120 kg/mu)+urea+water
3 processing are established in test altogether, repeat for 3 times, and totally 9 residential quarters, random district group is arranged residential quarter area 20m2.
4, test method:
(1) prepare bacterium liquid or solution:
Process 1.: in recommendation ratio (a kind of soluble straw decomposing agent, its: water: urea=150: 45000: 4000) (weight ratio) preparation bacterium liquid.
Process 2. CK I and process 3. CK II: do not add a kind of soluble straw decomposing agent, its, in recommendation ratio (water: urea=45000: 4000) (weight ratio) obtain solution.
(2) main control measures and method: this test divides two stages to carry out: the fs is that straw soaks and macerates Decomposition stage.According to testing program, process 1.: November 6 machine is received also field of the total straw pulverized, the amount wiring solution-forming of a kind of soluble straw decomposing agent, its+water+urea by 150 g/acres+45000 g/acres+4000 g/acres evenly sprayed on straw.Poured water 7 centimeters November 7, straw is soaked macerate decomposition; Process 2. CK I: the total straw of machine receive being pulverized November 6 is the field also, and the amount wiring solution-forming of water+urea by 45000 g/acres+4000 g/acres evenly sprayed on straw, uses the mechanical ramming straw again.Poured water 7 centimeters November 7, straw is soaked macerate decomposition; Process 3. CK II: the straw of machine receive being pulverized November 6 all pulls away, and straw is not executed in the field, and just water+urea evenly sprays in the field by 45000 g/acres+4000 g/acres amount wiring solution-forming.Poured water 7 centimeters November 7.The situation of observing the straw decomposition every 2 days: water colour, dry color, feel, stretching resistance, smell.When the fs finishes, measure the conventional index of respectively processing soil.Subordinate phase is manure trial.Each processes the whole ground of beginning to turn over December 2, and the straw of decomposition is turned under the soil, uses base manure in conjunction with each processing of whole ground: 150 kg/acres of fertilizers.In sowing on December 4, application rate is 450 g/acres for the examination cabbage heart.Imposed composite fertilizer December 18: 10 kg/acres.The cultivation management measures such as each thinning of processing, irrigation, fertilising, the control of sick Chinese caterpillar fungus, intertill and clean tillage are identical.The experimental plot cabbage heart began to gather on 01 7th, 2011, gathered separately, weighs separately in gradation when gathering, a minute residential quarter, gathered and finished rear each cell production of cumulative calculation.
5, test-results:
5.1 different treatment is on the impact of straw decomposition speed.The experimental observation result is as shown in table 1.
Table 1: different treatment straw decomposition condition survey table
As seen from Table 1: process 1. straw to the deliquescing in 7 days; And the processing of not executing stalk rotting agent 2. CK I straw to the 14 talentes become slightly soft; 7 to the 10 day time of straw that adds a kind of soluble straw decomposing agent, its and process 1. is degraded substantially; 28 to the 30 day time of straw of CK I just is degraded substantially and do not execute straw decomposing inoculant and process 2..
5.2 different treatment is on the impact of soil physical and chemical property
Different treatment is as shown in table 2 below on the impact of soil physical and chemical property.
Show after 2:28 days different treatment to the impact of soil physical and chemical property
As can be seen from Table 2: straw-returning was measured the proterties such as organic matter of soil in 28 days afterwards, and the result is: the processing of the using a kind of soluble straw decomposing agent, its 1. soil organism improves 2.19gkg
-1, full N improves 0.09gkg
-1, hydrolyzable nitrogen improves 2mgkg
-1, available phosphorus improves 1.5mgkg
-1, available potassium improves 2mgkg
-1Process 2. and be respectively with the increasing amount of processing the soil organism 3., full N, hydrolyzable nitrogen, available phosphorus, available potassium: 0.8mgkg
-1,-0.11mgkg
-1,-1mgkg
-1, 0.3mgkg
-1, 0.2mgkg
-1-0.2mgkg
-1,-0.02mgkg
-1, 0.6mgkg
-1-0.1mgkg
-1,-0.2mgkg
-1The processing of using a kind of soluble straw decomposing agent, its 1. with process 2., process 3., compare, the increasing amount of the soil organism, full N, hydrolyzable nitrogen, available phosphorus, available potassium all reaches conspicuous level.
5.3 different treatment is on the impact of cabbage heart economic characters
Different treatment affects the result shown in table 3 and table 4 to the cabbage heart economic characters.As can be seen from Table 3, the processing of using a kind of soluble straw decomposing agent, its is and Ye Kuanyou obvious promoter action long to plant height, the leaf of cabbage heart 1., process and 1. 3. to compare with the processing that does not have straw-returning, plant height, leaf is long and the wide average increase by 3.2 of leaf, 2.4 and 1.4 centimetres; With straw-returning without the processing of stalk rotting agent 2. compare plant height, leaf is long and the wide average increase by 2 of leaf, 0.9 and 1.2 centimetre.Difference reaches conspicuous level.
Table 3: the impact that different treatment is grown Growth of Brassica Parachinensis (unit: centimetre)
Table 4: different treatment is on the impact of yield of flowering Chinese cabbage
As can be seen from Table 4: use a kind of processing of soluble straw decomposing agent, its straw-returning 1., yield of flowering Chinese cabbage increases obviously, processes and 1. 3. compares with the processing that does not have straw-returning, and yield of flowering Chinese cabbage increases by 12.8%; 2. compare without the processing of stalk rotting agent with straw-returning, yield of flowering Chinese cabbage increases by 9.7%.Analysis of variance all reaches conspicuous level.
6 conclusion (of pressure testing)s
6.1 a soluble straw decomposing agent, its is conducive to accelerate soaking of straw and macerates decomposition speed.Rice straw also the field use a kind of soluble straw decomposing agent, its straw by the time of decomposition deliquescing than fast 15 to 20 days without straw decomposing inoculant.
6.2 the late rice straw-returning uses a kind of soluble straw decomposing agent, its can significantly promote growing of cabbage heart, can significantly improve the output of cabbage heart.With compare without straw decomposing inoculant, the output of cabbage heart increases by 9.7%.Difference reaches utmost point conspicuous level.
6.3 late rice straw-returning kind cabbage heart uses a kind of soluble straw decomposing agent, its, for the degradation speed that accelerates straw, improves the soil organism, promotes growing of cabbage heart, there is good effect the aspects such as output that improve cabbage heart.Be worth large scale application.
Claims (8)
1. a soluble straw decomposing agent, its is characterized in that, described straw decomposing inoculant is powder dose-type, can be entirely water-soluble, be grouped into by following one-tenth by weight:
Composite fungus agent 10-20 part;
Nutritive ingredient 20-50 part;
Plant source wedding agent 2-5 part;
Soluble carrier 25-68 part;
Wherein, described composite fungus agent comprises trichoderma harziarum 0.2-1.0 hundred million cfu/g, aspergillus niger 0.5-2.0 hundred million cfu/g, aspergillus oryzae 0.5-2.0 hundred million cfu/g, subtilis 1-5 hundred million cfu/g, Bacillus licheniformis 1-5 hundred million cfu/g;
Described nutritive ingredient is white sugar, brown sugar (Saccharum Sinensis Roxb.), and starch, protein powder is with iron, boron, zinc, copper, manganese and the molybdenum of amino acid and/or humic acid chelating; Described nutritive ingredient is with white sugar, brown sugar (Saccharum Sinensis Roxb.), and starch, protein powder with iron, boron, zinc, copper, manganese and the molybdenum of amino acid and/or humic acid chelating, mixes according to the ratio of 1:2.8:5:1:0.2 and to make;
Described plant source wedding agent is the Oil-tea-cake extractive substance.
2. straw decomposing inoculant according to claim 1 is characterized in that, it is grouped into by following one-tenth by weight:
Composite fungus agent 12-18 part;
Nutritive ingredient 22-45 part;
Plant source wedding agent 2-4 part;
Soluble carrier 33-64 part.
3. straw decomposing inoculant according to claim 2 is characterized in that, it is grouped into by following one-tenth by weight:
15 parts of composite fungus agents;
25 parts of nutritive ingredients;
3 parts of plant source wedding agents;
57 parts of soluble carrier.
4. each described soluble straw decomposing agent, its according to claim 1-3, it is characterized in that, described composite fungus agent comprises trichoderma harziarum 0.5 hundred million cfu/g, aspergillus niger 100,000,000 cfu/g, aspergillus oryzae 100,000,000 cfu/g, subtilis 500,000,000 cfu/g, Bacillus licheniformis 500,000,000 cfu/g.
5. each described soluble straw decomposing agent, its is characterized in that according to claim 1-3, and described soluble carrier is Zulkovsky starch or wilkinite.
6. soluble straw decomposing agent, its according to claim 1 is characterized in that, described composite fungus agent comprises the steps to prepare:
Bacterium mixes the preparation of spore powder:
With subtilis and Bacillus licheniformis, original strain carries out successively respectively under aseptic condition that slant culture, shaking table are cultivated, after the fermentor cultivation, with the fermented liquid that obtains by etc. weight mix, being prepared into bacterium through concentrate drying mixes spore powder;
Fungi mixes the preparation of spore powder:
With trichoderma harziarum, aspergillus niger, aspergillus niger, original strain carries out respectively slant culture, shaking table cultivation, fermentor cultivation, solid fermentation product spore successively under aseptic condition after, the substratum that produces spore fully that obtains is immersed in respectively makes spore suspension in the clear water, thereby obtain trichoderma harziarum spore suspension, aspergillus niger spore suspension, aspergillus oryzae spore suspension, then with the trichoderma harziarum spore suspension that obtains, aspergillus niger spore suspension, aspergillus oryzae spore suspension respectively concentrate drying be prepared into the fungal spore powder; Then, be hybridly prepared into fungi according to the 1:1:1 ratio and mix spore powder;
The above-mentioned bacterium mixing spore powder that makes and fungi are mixed spore powder according to the ratio mixing of 4:1, thereby prepare composite fungus agent.
7. preparation method who prepares each described soluble straw decomposing agent, its among the claim 1-6, it is according to aforementioned proportion semen multiplex powder, nutritive ingredient, plant source wedding agent, soluble carrier mixing and stirring to be prepared.
8. each described soluble straw decomposing agent, its application in crops straw returning field of claim 1-6, it is characterized in that, the straw decomposing inoculant of this solubility is powder dose-type, can be entirely water-soluble, in field or stalk composting, can spread fertilizer over the fields and can spray, be applicable to mechanized.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110199404 CN102351578B (en) | 2011-07-15 | 2011-07-15 | Soluble straw decomposing agent, preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110199404 CN102351578B (en) | 2011-07-15 | 2011-07-15 | Soluble straw decomposing agent, preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102351578A CN102351578A (en) | 2012-02-15 |
| CN102351578B true CN102351578B (en) | 2013-02-13 |
Family
ID=45575254
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110199404 Active CN102351578B (en) | 2011-07-15 | 2011-07-15 | Soluble straw decomposing agent, preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102351578B (en) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102674961B (en) * | 2012-05-05 | 2013-09-04 | 管天球 | Organic fertilizer produced from tea cakes |
| CN102876576B (en) * | 2012-09-24 | 2014-02-19 | 湖南泰谷生物科技有限责任公司 | Acidic soil conditioner, and preparation method and application thereof |
| CN103230917B (en) * | 2013-03-14 | 2015-04-29 | 东北林业大学 | Forest floor combustible biodegradation agent and use thereof |
| CN103214317B (en) * | 2013-05-16 | 2014-07-09 | 湖南泰谷生物科技股份有限公司 | Active amino acid water-soluble fertilizer as well as preparation method and application thereof |
| CN103342609B (en) * | 2013-07-17 | 2015-03-25 | 成都华隆生物科技有限公司 | Straw thoroughly-decomposing microbial inoculum capable of being preserved for long time under normal temperature condition |
| CN103351184B (en) * | 2013-07-17 | 2014-12-17 | 成都华隆生物科技有限公司 | Addition agent for accelerating straw decomposition |
| CN104193430A (en) * | 2014-08-27 | 2014-12-10 | 武汉瑞泽园生物环保科技有限公司 | Straw rotting agent and preparation method thereof |
| CN104692961A (en) * | 2015-02-03 | 2015-06-10 | 新疆天物生态科技股份有限公司 | Microorganism organic material decomposition agent as well as preparation method and applications thereof |
| CN104649761B (en) * | 2015-02-04 | 2017-09-01 | 中国烟草总公司广东省公司 | The high carbon-based additive of soil that a kind of rape stalk that ferments is prepared |
| CN104649758B (en) * | 2015-02-04 | 2017-08-29 | 中国烟草总公司广东省公司 | The high carbon-based additive of soil that a kind of rice straw that ferments is prepared |
| CN105331563A (en) * | 2015-12-08 | 2016-02-17 | 湖北正佳微生物工程股份有限公司 | Microorganism bacterium agent for straw thorough decomposition and preparation method thereof |
| CN105837267A (en) * | 2016-03-21 | 2016-08-10 | 宁夏五丰农业科技有限公司 | Preparation and use method of soluble solid straw decomposition agent |
| CN106986670A (en) * | 2017-05-19 | 2017-07-28 | 上海绿博生物科技发展有限公司 | A kind of biological agent for promoting returning to the field rice straw to become thoroughly decomposed and preparation method thereof |
| CN109403580B (en) * | 2018-10-22 | 2019-08-16 | 嘉善汇佳乐装饰材料有限公司 | A kind of environment Protection composite floor board based on degradable raw material |
| CN110076180B (en) * | 2019-05-14 | 2022-03-15 | 汪家权 | Domestic garbage aerobic high-temperature fermentation accelerant and preparation and application methods thereof |
| CN112189535A (en) * | 2020-09-10 | 2021-01-08 | 襄阳职业技术学院 | Pepper seedling raising substrate and preparation method thereof |
| CN112450027A (en) * | 2020-11-10 | 2021-03-09 | 广东省科学院生物工程研究所 | Sugarcane light and simple cultivation management method for rapid decomposition of sugarcane leaves by full coverage and returning to fields |
| CN112683723B (en) * | 2020-12-28 | 2023-10-27 | 淮阴师范学院 | Quantitative analysis method for anaerobic decomposition strength in straw returning soil |
| CN113135787A (en) * | 2021-03-01 | 2021-07-20 | 黑龙江省农业科学院畜牧研究所 | Rice straw decomposition agent and preparation method thereof |
| CN115466140B (en) * | 2022-10-20 | 2023-09-22 | 轩凯生物科技(山东)有限公司 | Straw decomposition agent for improving moisture uniformity of organic fertilizer stack and application thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1245501C (en) * | 2003-12-01 | 2006-03-15 | 华中农业大学 | Decomposed leaven for the treatment and use of industrial waste liquid and waste residue and method for preparing the same |
| CN101139561A (en) * | 2007-07-06 | 2008-03-12 | 李海泉 | Decomposition maturing agent for fast decomposition-maturing straw |
| CN101885624B (en) * | 2010-07-16 | 2012-09-19 | 湖北金帝农业科技有限公司 | Decomposition maturing agent for degrading straw |
-
2011
- 2011-07-15 CN CN 201110199404 patent/CN102351578B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN102351578A (en) | 2012-02-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102351578B (en) | Soluble straw decomposing agent, preparation method and application thereof | |
| CN103819267B (en) | For the microbial-bacterial fertilizer and preparation method thereof of peanut cultivation | |
| CN104177137B (en) | A kind of microbial straw composing agent and preparation method thereof | |
| CN102964178B (en) | Composite microbial bactericide, and preparation method and application thereof | |
| CN103819237B (en) | Tobacco special microorganism is fertile and preparation method thereof | |
| CN103342604B (en) | Bio-control bacterium foliage fertilizer, and preparation method and application thereof | |
| CN103964937B (en) | A kind of special tea seed cake fertilizer of Oryza sativa L. | |
| CN103086771B (en) | Bioorganic fertilizer preparation method, bioorganic fertilizer and application of bioorganic fertilizer | |
| CN101691549B (en) | Antagonistic bacteria capable of preventing and curing continuous cropping melon blast disease and microorganism organic fertilizer thereof | |
| CN103740693B (en) | A kind of straw decomposing inoculant and preparation method thereof | |
| CN106495826A (en) | A kind of production method of biological bacteria fruit tree drip irrigation fertilizer | |
| CN103964946A (en) | Special compound microbial fertilizer for paddy rice and preparation method thereof | |
| CN103518781B (en) | Core bio-control bacteria and organic fertilizer containing the core bio-control bacteria | |
| CN102876576A (en) | Acidic soil conditioner, and preparation method and application thereof | |
| CN101798243A (en) | Method for manufacturing and using microbial organic/inorganic compound fertilizer | |
| CN103242083A (en) | Microbial organic fertilizer | |
| CN105924317A (en) | Composite microbial fertilizer, and preparation method and application thereof | |
| CN103880487A (en) | Three-in-one green manure fermentation nutritional agent and application method thereof | |
| CN102731213A (en) | Fertilizer for cultivation of tobacco, preparation method and application thereof | |
| CN109734524A (en) | A kind of ecological system fertilizer of biologic ferment and the preparation method and application thereof | |
| CN106242829A (en) | A kind of overcome the soil remediation fertilizer of Radix Salviae Miltiorrhizae continuous cropping obstacle, preparation method and its usage | |
| CN106518362A (en) | Compound microbial fertilizer with continuous cropping resistance and preparation method of fertilizer | |
| CN104478565A (en) | Ferment liquid fertilizer and preparation method thereof | |
| CN106278654A (en) | A kind of overcome the soil remediation fertilizer of Rhizoma Zingiberis Recens continuous cropping obstacle, preparation method and its usage | |
| CN105732136A (en) | Special bio-organic fertilizer for pepper and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: HUNAN TAIGU BIOTECHNOLOGY CO.,LTD. Free format text: FORMER NAME: HUNAN TAIGU BIOLOGY TECHNOLOGY CO., LTD. |
|
| CP01 | Change in the name or title of a patent holder |
Address after: 410205 Hunan high tech Zone Changsha Lulong Road No. 199 building 1403 mark Lugu landmark A Patentee after: Hunan Taigu Biotechnology Co., Ltd. Address before: 410205 Hunan high tech Zone Changsha Lulong Road No. 199 building 1403 mark Lugu landmark A Patentee before: Hunan Taigu Biological Technology Co.,Ltd. |
|
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20120215 Assignee: Hunan Tai Gu bio-feritlizer company limited Assignor: Hunan Taigu Biotechnology Co., Ltd. Contract record no.: 2013430000023 Denomination of invention: Soluble straw decomposing agent, its preparation method and application Granted publication date: 20130213 License type: Exclusive License Record date: 20130410 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Soluble straw decomposing agent, its preparation method and application Effective date of registration: 20150907 Granted publication date: 20130213 Pledgee: Hunan Wangcheng Rural Commercial Bank branch Limited by Share Ltd Lugu Pledgor: Hunan Taigu Biotechnology Co., Ltd. Registration number: 2015430000021 |
|
| PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model | ||
| EC01 | Cancellation of recordation of patent licensing contract |
Assignee: Hunan Tai Gu bio-feritlizer company limited Assignor: Hunan Taigu Biotechnology Co., Ltd. Contract record no.: 2013430000023 Date of cancellation: 20150824 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20160829 Granted publication date: 20130213 Pledgee: Hunan Wangcheng Rural Commercial Bank branch Limited by Share Ltd Lugu Pledgor: Hunan Taigu Biotechnology Co., Ltd. Registration number: 2015430000021 |
|
| PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Soluble straw decomposing agent, its preparation method and application Effective date of registration: 20160902 Granted publication date: 20130213 Pledgee: Hunan Wangcheng Rural Commercial Bank branch Limited by Share Ltd Lugu Pledgor: Hunan Taigu Biotechnology Co., Ltd. Registration number: 2016430000031 |
|
| PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model | ||
| PM01 | Change of the registration of the contract for pledge of patent right |
Change date: 20170731 Registration number: 2016430000031 Pledgee after: Changsha rural commercial bank Limited by Share Ltd Lugu branch Pledgee before: Hunan Wangcheng Rural Commercial Bank branch Limited by Share Ltd Lugu |
|
| PM01 | Change of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20170809 Granted publication date: 20130213 Pledgee: Changsha rural commercial bank Limited by Share Ltd Lugu branch Pledgor: Hunan Taigu Biotechnology Co., Ltd. Registration number: 2016430000031 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Soluble straw decomposing agent, its preparation method and application Effective date of registration: 20170816 Granted publication date: 20130213 Pledgee: Changsha rural commercial bank Limited by Share Ltd Lugu branch Pledgor: Hunan Taigu Biotechnology Co., Ltd. Registration number: 2017430000054 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20180706 Granted publication date: 20130213 Pledgee: Changsha rural commercial bank Limited by Share Ltd Lugu branch Pledgor: Hunan Taigu Biotechnology Co., Ltd. Registration number: 2017430000054 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| CP03 | Change of name, title or address |
Address after: 410000 Hunan Changsha national hi tech Industrial Development Zone, 27 Yuan Lu Yu Yuan Pioneer Building 1506, 1507 Patentee after: Taigu ecological science and technology group Limited by Share Ltd Address before: 410205 A landmark 1403, Lulong road 199, Changsha new and high tech Zone, Hunan Patentee before: Hunan Taigu Biotechnology Co., Ltd. |
|
| CP03 | Change of name, title or address | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Soluble straw decomposing agent, its preparation method and application Effective date of registration: 20180813 Granted publication date: 20130213 Pledgee: Changsha bank Limited by Share Ltd Xiangjiang New District sub branch Pledgor: Taigu ecological science and technology group Limited by Share Ltd Registration number: 2018430000056 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |