Being used for the industrial organic waste liquid waste residue handles and become thoroughly decomposed starter and the production method thereof utilized
Technical field
The present invention relates to a kind of industrial organic waste liquid waste residue that is used for and handle and become thoroughly decomposed starter and the production method thereof utilized, belong to the starter production technical field.The present invention is applicable to that the temperature that requires to have fermented is fast, cycle is short, abilities such as decomposition of cellulose, hemicellulose, xylogen, protein are strong, the material high occasion of degree of becoming thoroughly decomposed, be specially adapted to cane sugar industry in liquid and waste slag produced comprehensive treating process such as the waste that produces in its production process such as molasses alcohol waste liquid and sugarcane marrow, filter mud and the industrial production of organic fertilizer material as the special use starter that becomes thoroughly decomposed.
Background technology
The comprehensive treating process of organic liquid waste waste residue and utilization are important topics of the environmental engineering and the utilization of resources, particularly aspect the sugar industry environmental protection treatment, refractory reason be molasses alcohol waste liquid and towards buck.To the processing of sugar industry alcohol slops and waste residue, the prior art scheme has both at home and abroad: agricultural filling method, oxidation pond process, biogas method, boiler flue circulate towards grey method, dignified sedimentation filtration method, method of enrichment, concentrated spray desiccating method, combustion method, make the fertile method of compound etc.Yet, these methods be not exist working cost height, energy consumption big, administer not thoroughly, have such-and-such problems such as operability is bad, secondary pollution exactly.In recent years, the favourable microorganism that exists with industrial organic waste liquid waste residue self carries out the technical scheme that thermophilic fermentation is handled under field conditions (factors), be called " utilizing molasses alcohol waste liquid to produce the active organic compound fertilizer method " (patent No.: patent 97112080) as the Chinese patent name, microbial function microbial inoculum (vinelandii, phosphate solubilizing bacteria, potassium solubilizing bacteria etc.) is provided when providing by waste self thermophilic fermentation, thereby increases fertilizer efficiency.This method has fermentation procedure, than being progress with molasses alcohol waste liquid dry powder with other fertilizer material mixing gained mixed fertilizer, but still has following shortcoming:
1, fermenting process is subjected to environmental influence bigger.Owing to utilize in the air and waste liquid, the entrained natural aerobic bacteria of waste residue self at high temperature ferment, the bacterial strain specific aim is poor, effectively the bacterium number is low, material can not ferment according to the fertilizer direction fully, the bacterial strain that wherein can play the fermentation maturity effect is limited, therefore, do not possessing under the sufficient processing condition situation, material can not get decomposing fully and becoming thoroughly decomposed;
2, for pending material can fully be become thoroughly decomposed, this treatment process is taked forced ventilation, repeatedly prepares burden and is turned over the ridge, and inserts septic plant residue and earth, so complex process, fermentation time long (38-71 days), running cost height;
3, fermented temperature slowly, foul smell is arranged, humic acids content is low, and fertilizer efficiency is relatively poor;
4, natural material microflora can change with season, weather effect, and fermenting process instability, quality product are also unstable;
5, the mode of production also need be set up ventilation equipment etc. simultaneously for to ferment in fermentation vat, thereby initial cost is big, the production cost height:
6, insert a large amount of clays (accounting for the 25-40% of fermentation materials total amount), the effective ingredient of fertilizer is low, and unit cost is handled the liquid and waste slag produced total amount of sugar industry can not be big.
Therefore, this top technology is not applied in production practice.
Also have the another kind of example that the functional microbial bacterial classification is produced microbial fertilizer that in waste liquid, waste residue material, adds in the prior art, as Chinese training application number be 01106646, name is called " utilizing filter mud of sugar refinery and waste residue and liquid to produce the method for microbial fertilizer ".The microbial strains of the disclosed interpolation of this method also is function bacterial classifications such as vinelandii, phosphate solubilizing bacteria, potassium solubilizing bacteria, its invention of adding such bacterial classification spells out in its specification sheets: such bacterial classification is to decompose to contain the material of phosphorus, potassium, so that become the utilizable phosphorus of crop, potassium; Vinelandii then be with in the soil and crop rhizosphere in the assimilation air nitrogen, for crop provide nitrogen and the secretion plant growth hormones.But this method mainly is to utilize sugar refinery waste to come culturing micro-organisms fertilizer as substratum, ultimate aim is to pursue the living bacteria count of various function yeast, in fact its substratum (waste in sugar refinery) direct returning to farmland under the situation of fully not decomposing and becoming thoroughly decomposed is not effectively handled sugar refinery waste.Therefore this method be not, can not carry out in a large number yet sugar refinery waste, economic, effectively improvement.
In sum, need a kind of economy, effective starter, so that realize the efficient processing of industrial organic waste liquid waste residue and fully utilize.
Summary of the invention
The objective of the invention is to, to provide a kind of fermentation to play temperature fast for the comprehensive treating process of organic liquid waste waste residue and utilization, cycle is short, decomposition of cellulose, this quality, starch, proteinic ability are strong, the material degree height that becomes thoroughly decomposed, fermenting process is not smelly, and the product after the fermentation is used for preparing high the become thoroughly decomposed starter and the production method thereof of fertilizer efficiency of fertilizer or biological organic fertilizer.
The technical scheme of the starter that becomes thoroughly decomposed that is used for the processing of industrial organic waste liquid waste residue and utilizes of the present invention is: it is made up of cellulose-decomposing bacterium, hemicellulose decomposer, protein decomposing bacteria, amylolysis bacterium; Described starter preferentially is selected from following bacterial classification or their combination: aspergillus niger (Aspergillus niger), viride (Trichoderma viride), Endomycopsis Fibnligera (Endomycopsis fibnliger), subtilis (Bacillus subtilis), cereuisiae fermentum (Saccharomyces cerevisiae); The bacterial classification capacity of decomposition is one of the following or respective combination: live 50~1000 milligrams of glucose/grams hour of CMC enzyme, 500~5000 milligrams of wood sugar/grams of xylanase activity hour, live 50~500 micrograms tyrosine/gram hour of neutral protease, 100~1000 milligrams of glucose/grams of saccharifying enzyme work hour.
The production method of the starter that becomes thoroughly decomposed that is used for the processing of industrial organic waste liquid waste residue and utilizes of the present invention is: at first collect and cultivate high cellulase-producing, hemicellulase, protein enzyme, enzyme starch etc. and produce bacterial strain, composition and technical parameter according to the starter that becomes thoroughly decomposed requires to be mixed with the starter that becomes thoroughly decomposed in collected bacterial strain then, and it comprises the steps:
1), produce the bacterial classification song:
(1) obtain three-class strain: with first class inoculum is the test tube kind, through the processing of switching enlarged culturing, obtains second class inoculum, i.e. triangular flask solid kind, then with second class inoculum through tortuous or plant bent machine enlarged culturing and obtain three-class strain, promptly produce kind;
(2) will be that the substratum of main raw material adds water with wheat bran, the sterilization of goal Guo Bamboo vapour be placed on spreading for cooling on the Ventilated bed then;
(3) inoculation: the production kind of above-mentioned the first step acquisition is inoculated on the sterilization raw material of spreading for cooling, ventilated and cultivate, obtain kind of a song;
(4) check: kind of a song is tested, and wherein, water content is 25~50%;
2), the rotten hot fermentation agent of preparation:
(1) step of the starter that becomes thoroughly decomposed of promptly using of preparation wet type is: with the above-mentioned the 1st) obtained various songs of step, mix by predetermined required bacterial classification combination requirement, promptly obtain the aqueous i.e. starter that becomes thoroughly decomposed of usefulness of promptly joining;
(2) obtained various Qu Jinhang oven drying at low temperatures of the step preparation dry type commodity starter that becomes thoroughly decomposed: with the above-mentioned the 1st) requiredly mix, dry with bacterial classification combination requirements by predetermined then, i.e. the dried commodity of the acquisition starter that becomes thoroughly decomposed;
3), bacterial classification is an aspergillus niger, viride, Endomycopsis Fibnligera, subtilis, cereuisiae fermentum, its CMC enzyme work are 100 milligrams of glucose/grams hour, and xylanase activity is 500 milligrams of wood sugar/grams hour, live 100 milligrams of glucose/grams hour of saccharifying enzyme, neutral protease work 50 micrograms tyrosine/gram hour; Substratum is: wheat bran 50%, corn cob 50%; Material-water ratio is 1: 1; Ventilate and cultivated 3 days, turn over bent 3~5 times, obtain to plant bent; The bent water content of kind that requires set by step to make is 40~50%.
Description of drawings
Temperature variation curve in Fig. 1, the composting process.Annotate: A is the temperature variation curve of starter A; B is the temperature variation curve of starter B; C is the temperature variation curve of starter C; X is the temperature variation curve of contrast.
Soil ulmin change curve in Fig. 2, the composting process.Annotate: A is the soil ulmin change curve of starter A; B is the soil ulmin change curve of starter B; C is the soil ulmin change curve of starter C; X is the soil ulmin change curve of contrast.
C/N change curve in Fig. 3, the composting process.Annotate: A is the c/N change curve of fermentation chaste tree A; B is the c/N change curve of starter B; C is the C/N change curve of starter C; X is the C/N change curve of contrast
Embodiment
The starter specific embodiment that becomes thoroughly decomposed that is used for the processing of industrial organic waste liquid waste residue and utilizes of the present invention is:
Embodiment 1.1: this example is described, and the starter that becomes thoroughly decomposed is formed by containing cellulose-decomposing bacterium, hemicellulose decomposer, protein decomposing bacteria, amylolysis bacterium; Described starter preferentially is selected from following bacterial classification or their combination: aspergillus niger (Aspergillus niger), viride (Trichoderma viride), Endomycopsis Fibnligera (Endomycopsis fibnliger), subtilis (Bacillus subtilis), cereuisiae fermentum (Saccharomyces cerevisiae); The bacterial classification capacity of decomposition is one of the following or respective combination: live 50~1000 milligrams of glucose/grams hour of CMC enzyme, 500~5000 milligrams of wood sugar/grams of xylanase activity hour, live 50~500 micrograms tyrosine/gram hour of neutral protease, 100~1000 milligrams of glucose/grams of saccharifying enzyme work hour; Wherein said bacterial classification is the arbitrary single bacterial strain of aspergillus niger, viride, Endomycopsis Fibnligera, subtilis and cereuisiae fermentum.
Embodiment 1.2: this example is described, and become thoroughly decomposed starter and embodiment 1.1 differences are: described bacterial classification is viride, Endomycopsis Fibnligera, live 1000 milligrams of glucose/grams hour of its CMC enzyme, 500 milligrams of glucose/grams of saccharifying enzyme work hour.
Embodiment 1.3: this example is described, and become thoroughly decomposed starter and embodiment 1.1 differences are: described bacterial classification is Endomycopsis Fibnligera, subtilis, live 1000 milligrams of glucose/grams hour of its saccharifying enzyme, neutral protease work 500 micrograms tyrosine/gram hour.
Embodiment 1.4: this example is described, and become thoroughly decomposed starter and embodiment 1.1 differences are: described bacterial classification is aspergillus niger, Endomycopsis Fibnligera, subtilis, its neutral protease activity 500 micrograms tyrosine/gram hour, 5000 milligrams of wood sugar/grams of xylanase activity hour.
Embodiment 1.5: this example is described, and become thoroughly decomposed starter and embodiment 1.1 differences are: described bacterial classification is Endomycopsis Fibnligera, aspergillus niger, viride, subtilis, cereuisiae fermentum, live 50 milligrams of glucose/grams hour of its CMC enzyme, 500 milligrams of wood sugar/grams of xylanase activity hour, live 100 milligrams of glucose/grams hour of saccharifying enzyme, neutral protease work 50 micrograms tyrosine/gram hour.
Embodiment 1.6: this example is described, and become thoroughly decomposed starter and embodiment 1.1 differences are: described bacterial classification is an aspergillus niger, viride, Endomycopsis Fibnligera, subtilis, cereuisiae fermentum is formed, and its CMC enzyme work is 500 milligrams of glucose/grams hour, and xylanase activity is 2000 milligrams of wood sugar/grams hour, live 800 milligrams of glucose/grams hour of saccharifying enzyme, neutral protease work 250 micrograms tyrosine/gram hour.
Realization of the present invention is used for the specific embodiment that the industrial organic waste liquid waste residue handles with the production method of the starter that becomes thoroughly decomposed that utilizes:
Embodiment 2.1: the production method of the described starter that becomes thoroughly decomposed of this example, at first collect and cultivate the generation bacterial strain of high cellulase-producing, hemicellulase, in collected bacterial strain, screen highly active decomposition bacterial strain then, composition requirement according to the starter that becomes thoroughly decomposed is mixed with the starter that becomes thoroughly decomposed at last, the steps include:
1), produce the bacterial classification song:
(1) obtain three-class strain: with first class inoculum is the test tube kind, through the processing of switching enlarged culturing, obtains second class inoculum, i.e. solid kind in the bottle, then with second class inoculum through tortuous or plant bent machine enlarged culturing and obtain three-class strain, promptly produce kind;
(2) will be that the substratum of raw material adds water with wheat bran and straw powder, the sterilization of goal Guo Bamboo vapour be placed on spreading for cooling on the Ventilated bed then;
(3) inoculation: the production kind of above-mentioned the first step acquisition is inoculated on the sterilization raw material of spreading for cooling, ventilated and cultivate, obtain kind of a song;
(4) check: kind of a song is tested, and wherein, water content is 25~50%;
2) prepare the starter that becomes thoroughly decomposed:
(1) step of the starter that becomes thoroughly decomposed of promptly using of preparation wet type is: with the above-mentioned the 1st) obtained various songs of step, mix by predetermined required bacterial classification combination requirement, promptly obtain the aqueous i.e. starter that becomes thoroughly decomposed of usefulness of promptly joining;
(2) obtained various Qu Jinhang oven drying at low temperatures of the step preparation dry type commodity starter that becomes thoroughly decomposed: with the above-mentioned the 1st) requiredly mix, dry with bacterial classification combination requirements by predetermined then, i.e. the dried commodity of the acquisition starter that becomes thoroughly decomposed;
3), the bacterial classification of the described starter that becomes thoroughly decomposed is aspergillus niger, viride, Endomycopsis Fibnligera, subtilis and cereuisiae fermentum, live 100 milligrams of glucose/grams hour of its CMC enzyme, 500 milligrams of wood sugar/grams of xylanase activity hour, live 50 micrograms tyrosine/gram hour of neutral protease, 100 milligrams of glucose/grams of saccharifying enzyme work hour; Substratum is wheat bran 50% and corn cob 50%, and material-water ratio is 1: 1; The cultivation of ventilating was turned over bent 3~5 times in 3 days and is obtained to plant bent; The bent water content of kind that requires set by step to make is 40~50%, and the bent water content of can choosing seeds is 40%.
Described bacterial classification can also be the arbitrary single bacterial strain of aspergillus niger, viride, Endomycopsis Fibnligera, subtilis and cereuisiae fermentum, and substratum is a wheat bran 50%, dregs of beans 10%, and straw powder 40%, material-water ratio are 1: 1; The cultivation of ventilating was turned over bent 2~3 times in 2 days and is obtained to plant bent; The bent water content of kind that requires set by step to make is 25~40%, and the bent water content of can choosing seeds is 35%.
Obvious technical effects of the present invention has realized purpose of the present invention.Below the starter that becomes thoroughly decomposed starter and prior art of the present invention is compared, technical progress of the present invention is given prominence to:
In Fig. 1, Fig. 2, Fig. 3:
Starter A is the starter that becomes thoroughly decomposed of embodiments of the invention 2.2
Starter B is the liquid state fermentation agent of the new Messrs.Far East ﹠ Co., Ltd of Canada
Starter C is the Japanese starter of Guangdong Jin Kui Subsidiary Company
Contrast X utilizes and reaches waste liquid, the entrained natural aerobic bacteria fermentation of waste residue self in the air
(1) measures content
Temperature measuring: the temperature of measuring donor center before turning every day
The mensuration of soil ulmin, C/N: the method by regulation in " soil physical analysis " (Shanghai science tech publishing house, 1978) is carried out.
Ocular estimate method: undertaken by the method for stipulating among " Shanghai environmental science " calendar year 2001 the 20th volume the 2nd phase P85-87.
Temperature variation curve contrast situation is seen Fig. 1, and soil ulmin changes the contrast situation and sees Fig. 2; C/N in the composting process
Change and see Fig. 3.
Other relevant evaluation situations, for example the compost ocular estimate result such as the following subordinate list of different fermentations agent production are put down in writing:
Starter | Ocular estimate |
Contrast | Chocolate; Frowziness, no humic breath; Attract a small amount of mosquitos and flies; Particle diameter is than the people, and the part caking is clamminess, and feel is thicker, gets 2 fens. |
A | Chocolate: no stink, significantly humic breath; Do not attract mosquitos and flies; The crumb structure that drought is loose, feel is thinner: place one day rear surface adularescent mycelial growth, got 9 fens. |
B | Chocolate; Slightly frowziness has the slight humic breath that gets; Do not attract mosquitos and flies: apparent more loose, a small amount of caking, feel is thick slightly, gets 5 fens. |
C | Chocolate; Slight stink, significantly humic breath; Do not attract mosquitos and flies: apparent more loose, the minute quantity caking; Placing two days rear surfaces has a small amount of mycelia, gets 7 fens. |
(4) starter comparing result
A, heat up from compost fermentation, starter A can reach 54 ℃ in second day, and than other several starters fast 1-3 days, high temperature (more than 60 ℃) can be kept 9 days, than other several starters long 2-5 days.
B, through behind 16 days compost fermentations, from the content of humic substances increase, starter A is increased to 17.7% from 1.9% of beginning, effect is best.Be followed successively by starter C>starter B>contrast.
C, from C/N, contrast and starter B, compost is after 16 days, C/N dropped to 20.9: 1 respectively and 20.2: 1 from 28: 1, used starter A, C, compost is after 16 days, from 16.5: 1 and 16.9: 1 of decline in 28: 1, effect was more satisfactory respectively for C/N.
D, from appearance index, the compost that starter A produces got 9 fens, the compost that starter C produces got 7 fens, the compost that starter B produces got 5 fens, contrast 2 minutes.
In sum: the present invention's leavening that becomes thoroughly decomposed is compared with the leavening of prior art, and comprehensive treatment capability is much better than, and effect is much better.
Method and technology effect of the present invention is, technique is simple, cost is low, constant product quality, to provide a kind of fermentation to play temperature fast for the integrated treatment of organic liquid waste waste residue and utilization, and the cycle is short, and the abilities such as decomposition of cellulose, hemicellulose, lignin, protein are strong, the material degree height that becomes thoroughly decomposed, the production method that sweat is not smelly.