CN1546400A - Decomposed leaven for the treatment and use of industrial waste liquid and waste residue and method for preparing the same - Google Patents
Decomposed leaven for the treatment and use of industrial waste liquid and waste residue and method for preparing the same Download PDFInfo
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- CN1546400A CN1546400A CNA2003101114779A CN200310111477A CN1546400A CN 1546400 A CN1546400 A CN 1546400A CN A2003101114779 A CNA2003101114779 A CN A2003101114779A CN 200310111477 A CN200310111477 A CN 200310111477A CN 1546400 A CN1546400 A CN 1546400A
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- 238000000034 method Methods 0.000 title abstract description 35
- 239000002699 waste material Substances 0.000 title description 24
- 239000007788 liquid Substances 0.000 title description 12
- 239000002440 industrial waste Substances 0.000 title 1
- 230000001580 bacterial effect Effects 0.000 claims abstract description 49
- 241000894006 Bacteria Species 0.000 claims abstract description 20
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 19
- 238000002360 preparation method Methods 0.000 claims abstract description 8
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 6
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 5
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 5
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 4
- 239000007858 starting material Substances 0.000 claims description 83
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- 229940088598 enzyme Drugs 0.000 claims description 25
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- 239000008103 glucose Substances 0.000 claims description 24
- 230000000694 effects Effects 0.000 claims description 20
- 238000004519 manufacturing process Methods 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 108090000145 Bacillolysin Proteins 0.000 claims description 13
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims description 13
- 102000035092 Neutral proteases Human genes 0.000 claims description 13
- 108091005507 Neutral proteases Proteins 0.000 claims description 13
- 239000010808 liquid waste Substances 0.000 claims description 13
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 claims description 13
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims description 11
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 claims description 11
- 229960003487 xylose Drugs 0.000 claims description 11
- 239000010815 organic waste Substances 0.000 claims description 10
- 238000012545 processing Methods 0.000 claims description 10
- 239000002775 capsule Substances 0.000 claims description 9
- 239000002054 inoculum Substances 0.000 claims description 9
- 241000196324 Embryophyta Species 0.000 claims description 8
- 238000000354 decomposition reaction Methods 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 7
- 235000015099 wheat brans Nutrition 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 238000003892 spreading Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 229920002488 Hemicellulose Polymers 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 108010059892 Cellulase Proteins 0.000 claims description 3
- 241000223261 Trichoderma viride Species 0.000 claims description 3
- 229940106157 cellulase Drugs 0.000 claims description 3
- 108010002430 hemicellulase Proteins 0.000 claims description 3
- 229940059442 hemicellulase Drugs 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 238000005304 joining Methods 0.000 claims description 3
- 238000000643 oven drying Methods 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 239000010902 straw Substances 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 2
- 240000008042 Zea mays Species 0.000 claims description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 2
- 235000005822 corn Nutrition 0.000 claims description 2
- 230000007306 turnover Effects 0.000 claims 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims 1
- 235000017491 Bambusa tulda Nutrition 0.000 claims 1
- 244000082204 Phyllostachys viridis Species 0.000 claims 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims 1
- 239000011425 bamboo Substances 0.000 claims 1
- 239000001913 cellulose Substances 0.000 abstract description 4
- 229920002678 cellulose Polymers 0.000 abstract description 4
- 238000000855 fermentation Methods 0.000 description 15
- 230000004151 fermentation Effects 0.000 description 15
- 239000003337 fertilizer Substances 0.000 description 12
- 239000000463 material Substances 0.000 description 11
- 239000002689 soil Substances 0.000 description 9
- 239000002361 compost Substances 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241000256113 Culicidae Species 0.000 description 4
- 241000255925 Diptera Species 0.000 description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 4
- 235000019219 chocolate Nutrition 0.000 description 4
- 238000009264 composting Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 239000011591 potassium Substances 0.000 description 4
- 229910052700 potassium Inorganic materials 0.000 description 4
- 230000003381 solubilizing effect Effects 0.000 description 4
- 229910019142 PO4 Inorganic materials 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 241001148470 aerobic bacillus Species 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 239000002893 slag Substances 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 206010009866 Cold sweat Diseases 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
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- 230000002349 favourable effect Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 239000004021 humic acid Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
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- -1 this quality Polymers 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention relates to a decomposed leaven for industrial organic effluent and debris treatment and process for preparation, wherein the decomposed leaven comprises one or more of cellulose disassembling bacterium, himicellulose disassembling bacterium, protein disassembling bacterium, and amylolyzing bacterium, the bacterial are preferably selected from the following bacterial or their combination, aspergillus niger, viridian, endomycopsis fibuliger, bacillus subtilis, and brewer's yeast. The preparation process of the decomposed leaven comprises two steps of preparing bacterial and formulating leaven.
Description
Technical field
The present invention relates to a kind of industrial organic waste liquid waste residue that is used for and handle and become thoroughly decomposed starter and the production method thereof utilized, belong to the starter production technical field.The present invention is applicable to that the temperature that requires to have fermented is fast, cycle is short, abilities such as decomposition of cellulose, hemicellulose, xylogen, protein are strong, the material high occasion of degree of becoming thoroughly decomposed, be specially adapted to cane sugar industry in liquid and waste slag produced comprehensive treating process such as the waste that produces in its production process such as molasses alcohol waste liquid and sugarcane marrow, filter mud and the industrial production of organic fertilizer material as the special use starter that becomes thoroughly decomposed.
Background technology
The comprehensive treating process of organic liquid waste waste residue and utilization are important topics of the environmental engineering and the utilization of resources, particularly aspect the sugar industry environmental protection treatment, refractory reason be molasses alcohol waste liquid and towards buck.To the processing of sugar industry alcohol slops and waste residue, the prior art scheme has both at home and abroad: agricultural filling method, oxidation pond process, biogas method, boiler flue circulate towards grey method, dignified sedimentation filtration method, method of enrichment, concentrated spray desiccating method, combustion method, make the fertile method of compound etc.Yet, these methods be not exist working cost height, energy consumption big, administer not thoroughly, have such-and-such problems such as operability is bad, secondary pollution exactly.In recent years, the favourable microorganism that exists with industrial organic waste liquid waste residue self carries out the technical scheme that thermophilic fermentation is handled under field conditions (factors), be called " utilizing molasses alcohol waste liquid to produce the active organic compound fertilizer method " (patent No.: patent 97112080) as the Chinese patent name, microbial function microbial inoculum (vinelandii, phosphate solubilizing bacteria, potassium solubilizing bacteria etc.) is provided when providing by waste self thermophilic fermentation, thereby increases fertilizer efficiency.This method has fermentation procedure, than being progress with molasses alcohol waste liquid dry powder with other fertilizer material mixing gained mixed fertilizer, but still has following shortcoming:
1, fermenting process is subjected to environmental influence bigger.Owing to utilize in the air and waste liquid, the entrained natural aerobic bacteria of waste residue self at high temperature ferment, the bacterial strain specific aim is poor, effectively the bacterium number is low, material can not ferment according to the fertilizer direction fully, the bacterial strain that wherein can play the fermentation maturity effect is limited, therefore, do not possessing under the sufficient processing condition situation, material can not get decomposing fully and becoming thoroughly decomposed;
2, for pending material can fully be become thoroughly decomposed, this treatment process is taked forced ventilation, repeatedly prepares burden and is turned over the ridge, and inserts septic plant residue and earth, so complex process, fermentation time long (38-71 days), running cost height;
3, fermented temperature slowly, foul smell is arranged, humic acids content is low, and fertilizer efficiency is relatively poor;
4, natural material microflora can change with season, weather effect, and fermenting process instability, quality product are also unstable;
5, the mode of production also need be set up ventilation equipment etc. simultaneously for to ferment in fermentation vat, thereby initial cost is big, the production cost height;
6, insert a large amount of clays (accounting for the 25-40% of fermentation materials total amount), the effective ingredient of fertilizer is low, and unit cost is handled the liquid and waste slag produced total amount of sugar industry can not be big.
Therefore, this technology is not applied in production practice.
Also have the another kind of example that the functional microbial bacterial classification is produced microbial fertilizer that in waste liquid, waste residue material, adds in the prior art, as Chinese training application number be 01106646, name is called " utilizing filter mud of sugar refinery and waste residue and liquid to produce the method for microbial fertilizer ".The microbial strains of the disclosed interpolation of this method also is function bacterial classifications such as vinelandii, phosphate solubilizing bacteria, potassium solubilizing bacteria, its invention of adding such bacterial classification spells out in its specification sheets: such bacterial classification is to decompose to contain the material of phosphorus, potassium, so that become the utilizable phosphorus of crop, potassium; Vinelandii then be with in the soil and crop rhizosphere in the assimilation air nitrogen, for crop provide nitrogen and the secretion plant growth hormones.But this method mainly is to utilize sugar refinery waste to come culturing micro-organisms fertilizer as substratum, ultimate aim is to pursue the living bacteria count of various function yeast, in fact its substratum (waste in sugar refinery) direct returning to farmland under the situation of fully not decomposing and becoming thoroughly decomposed is not effectively handled sugar refinery waste.Therefore this method be not, can not carry out in a large number yet sugar refinery waste, economic, effectively improvement.
In sum, need a kind of economy, effective starter, so that realize the efficient processing of industrial organic waste liquid waste residue and fully utilize.
Summary of the invention
The objective of the invention is to, to provide a kind of fermentation to play temperature fast for the comprehensive treating process of organic liquid waste waste residue and utilization, cycle is short, decomposition of cellulose, this quality, starch, proteinic ability are strong, the material degree height that becomes thoroughly decomposed, fermenting process is not smelly, and the product after the fermentation is used for preparing high the become thoroughly decomposed starter and the production method thereof of fertilizer efficiency of fertilizer or biological organic fertilizer.
The technical scheme of the starter that becomes thoroughly decomposed that is used for the processing of industrial organic waste liquid waste residue and utilizes of the present invention is: it is made up of cellulose-decomposing bacterium, hemicellulose decomposer, protein decomposing bacteria, amylolysis bacterium; Described starter preferentially is selected from following bacterial classification or their combination: aspergillus niger (Aspergillus niger), viride (Trichoderma viride), Endomycopsis Fibnligera (Endomycopsis fibnliger), subtilis (Bacillus subtilis), cereuisiae fermentum (Saccharomyces cerevisiae); The bacterial classification capacity of decomposition is one of the following or respective combination: live 50～1000 milligrams of glucose/grams hour of CMC enzyme, 500～5000 milligrams of wood sugar/grams of xylanase activity hour, live 50～500 micrograms tyrosine/gram hour of neutral protease, 100～1000 milligrams of glucose/grams of saccharifying enzyme work hour.
The production method of the starter that becomes thoroughly decomposed that is used for the processing of industrial organic waste liquid waste residue and utilizes of the present invention is: at first collect and cultivate high cellulase-producing, hemicellulase, protein enzyme, enzyme starch etc. and produce bacterial strain, composition and technical parameter according to the starter that becomes thoroughly decomposed requires to be mixed with the starter that becomes thoroughly decomposed in collected bacterial strain then, and it comprises the steps:
1), produce the bacterial classification song:
(1) obtain three-class strain: with first class inoculum is the test tube kind, through the processing of switching enlarged culturing, obtains second class inoculum, i.e. triangular flask solid kind, then with second class inoculum through tortuous or plant bent machine enlarged culturing and obtain three-class strain, promptly produce kind;
(2) will be that the substratum of main raw material(s) adds water with wheat bran, goal pot steam sterilizing be placed on spreading for cooling on the Ventilated bed then;
(3) inoculation: the production kind of above-mentioned the first step acquisition is inoculated on the sterilization raw material of spreading for cooling, ventilated and cultivate, obtain kind of a song;
(4) check: purebred song is tested, and wherein, water content is 25～50%;
2) prepare the starter that becomes thoroughly decomposed:
(1) step of the starter that becomes thoroughly decomposed of promptly using of preparation wet type is: with the above-mentioned the 1st) each obtained purebred song of step, mix by predetermined required bacterial classification combination requirement, promptly obtain the aqueous i.e. starter that becomes thoroughly decomposed of usefulness of promptly joining;
(2) each obtained purebred Qu Jinhang oven drying at low temperature of the step preparation dry type commodity starter that becomes thoroughly decomposed: with the above-mentioned the 1st) requiredly mixes, dries with bacterial classification combination requirements by predetermined then, i.e. the dried commodity of the acquisition starter that becomes thoroughly decomposed.
Description of drawings
Temperature variation curve in Fig. 1, the composting process.Annotate: A is the temperature variation curve of starter A; B is the temperature variation curve of starter B; C is the temperature variation curve of starter C; X is the temperature variation curve of contrast.
Soil ulmin change curve in Fig. 2, the composting process.Annotate: A is the soil ulmin change curve of starter A; B is the soil ulmin change curve of starter B; C is the soil ulmin change curve of starter C; X is the soil ulmin change curve of contrast.
C/N change curve in Fig. 3, the composting process.Annotate: A is the c/N change curve of starter A; B is the c/N change curve of starter B; C is the C/N change curve of starter C; X is the C/N change curve of contrast
Embodiment
The starter specific embodiment that becomes thoroughly decomposed that is used for the processing of industrial organic waste liquid waste residue and utilizes of the present invention is:
Embodiment 1.1: this example is described, and the starter that becomes thoroughly decomposed is formed by containing cellulose-decomposing bacterium, hemicellulose decomposer, protein decomposing bacteria, amylolysis bacterium; Described starter preferentially is selected from following bacterial classification or their combination: aspergillus niger (Aspergillus niger), viride (Trichoderma viride), Endomycopsis Fibnligera (Endomycopsis fibnliger), subtilis (Bacillus subtilis), cereuisiae fermentum (Saccharomyces cerevisiae); The bacterial classification capacity of decomposition is one of the following or respective combination: live 50～1000 milligrams of glucose/grams hour of CMC enzyme, 500～5000 milligrams of wood sugar/grams of xylanase activity hour, live 50～500 micrograms tyrosine/gram hour of neutral protease, 100～1000 milligrams of glucose/grams of saccharifying enzyme work hour; Wherein said bacterial classification is the arbitrary single bacterial strain of aspergillus niger, viride, Endomycopsis Fibnligera, subtilis and cereuisiae fermentum.
Embodiment 1.2: this example is described, and become thoroughly decomposed starter and embodiment 1.1 differences are: described bacterial classification is viride, Endomycopsis Fibnligera, live 1000 milligrams of glucose/grams hour of its CMC enzyme, 500 milligrams of glucose/grams of saccharifying enzyme work hour.
Embodiment 1.3: this example is described, and become thoroughly decomposed starter and embodiment 1.1 differences are: described bacterial classification is Endomycopsis Fibnligera, subtilis, live 1000 milligrams of glucose/grams hour of its saccharifying enzyme, neutral protease work 500 micrograms tyrosine/gram hour.
Embodiment 1.4: this example is described, and become thoroughly decomposed starter and embodiment 1.1 differences are: described bacterial classification is aspergillus niger, Endomycopsis Fibnligera, subtilis, its neutral protease activity 500 micrograms tyrosine/gram hour, 5000 milligrams of wood sugar/grams of xylanase activity hour.
Embodiment 1.5: this example is described, and become thoroughly decomposed starter and embodiment 1.1 differences are: described bacterial classification is Endomycopsis Fibnligera, aspergillus niger, viride, subtilis, cereuisiae fermentum, live 50 milligrams of glucose/grams hour of its CMC enzyme, 500 milligrams of wood sugar/grams of xylanase activity hour, live 100 milligrams of glucose/grams hour of saccharifying enzyme, neutral protease work 50 micrograms tyrosine/gram hour.
Embodiment 1.6: this example is described, and become thoroughly decomposed starter and embodiment 1.1 differences are: described bacterial classification is an aspergillus niger, viride, it is mould that the button capsule is intended spore, subtilis, cereuisiae fermentum is formed, and its CMC enzyme work is 500 milligrams of glucose/grams hour, and xylanase activity is 2000 milligrams of wood sugar/grams hour, live 800 milligrams of glucose/grams hour of saccharifying enzyme, neutral protease work 250 micrograms tyrosine/gram hour.
Realization of the present invention is used for the industrial organic waste liquid waste residue and handles concrete with the production method of the starter that becomes thoroughly decomposed that utilizes
Embodiment is:
Embodiment 2.1: the production method of the described starter that becomes thoroughly decomposed of this example, at first collect and cultivate the generation bacterial strain of high cellulase-producing, hemicellulase, in collected bacterial strain, screen highly active decomposition bacterial strain then, composition requirement according to the starter that becomes thoroughly decomposed is mixed with the starter that becomes thoroughly decomposed at last, the steps include:
1), produce the bacterial classification song:
(1) obtain three-class strain: with first class inoculum is the test tube kind, through the processing of switching enlarged culturing, obtains second class inoculum, i.e. solid kind in the bottle, then with second class inoculum through tortuous or plant bent machine enlarged culturing and obtain three-class strain, promptly produce kind;
(2) will be that raw-material substratum adds water with wheat bran and straw powder, goal pot steam sterilizing be placed on spreading for cooling on the Ventilated bed then;
(3) inoculation: the production kind of above-mentioned the first step acquisition is inoculated on the sterilization raw material of spreading for cooling, ventilated and cultivate, obtain kind of a song;
(4) check: purebred song is tested, and wherein, water content is 25～50%;
2) prepare the starter that becomes thoroughly decomposed:
(1) step of the starter that becomes thoroughly decomposed of promptly using of preparation wet type is: with the above-mentioned the 1st) each obtained purebred song of step, mix by predetermined required bacterial classification combination requirement, promptly obtain the aqueous i.e. starter that becomes thoroughly decomposed of usefulness of promptly joining;
(2) each obtained purebred Qu Jinhang oven drying at low temperature of the step preparation dry type commodity starter that becomes thoroughly decomposed: with the above-mentioned the 1st) requiredly mixes, dries with bacterial classification combination requirements by predetermined then, i.e. the dried commodity of the acquisition starter that becomes thoroughly decomposed.
Bacterial classification described in the present embodiment is the arbitrary single bacterial strain of aspergillus niger, viride, Endomycopsis Fibnligera, subtilis and cereuisiae fermentum, and substratum is a wheat bran 50%, dregs of beans 10%, and straw powder 40%, material-water ratio are 1: 1; The cultivation of ventilating was turned over bent 2～3 times in 2 days and is obtained to plant bent; The purebred bent water content that requires set by step to make is 25～40%, and optional purebred bent water content is 35%.
Embodiment 2.2: the production method of the described starter that becomes thoroughly decomposed of this example and embodiment 2.1 differences are: the bacterial classification of the described starter that becomes thoroughly decomposed is aspergillus niger, viride, Endomycopsis Fibnligera, subtilis and cereuisiae fermentum, live 100 milligrams of glucose/grams hour of its CMC enzyme, 500 milligrams of wood sugar/grams of xylanase activity hour, live 50 micrograms tyrosine/gram hour of neutral protease, 100 milligrams of glucose/grams of saccharifying enzyme work hour; Substratum is wheat bran 50% and corn cob 50%, and material-water ratio is 1: 1; The cultivation of ventilating was turned over bent 3～5 times in 3 days and is obtained to plant bent; The purebred bent water content that requires set by step to make is 40～50%, and optional purebred bent water content is 40%.
Obvious technical effects of the present invention has realized purpose of the present invention.Below the starter that becomes thoroughly decomposed starter and prior art of the present invention is compared, technical progress of the present invention is given prominence to:
In Fig. 1, Fig. 2, Fig. 3:
Starter A is the starter that becomes thoroughly decomposed of embodiments of the invention 2.2
Starter B is the liquid state fermentation agent of the new Messrs.Far East ﹠ Co., Ltd of Canada
Starter C is the Japanese starter of Guangdong Jin Kui Subsidiary Company
Contrast X utilizes and reaches waste liquid, the entrained natural aerobic bacteria fermentation of waste residue self in the air
(1) measures content
Temperature measuring: the temperature of measuring donor center before turning every day
The mensuration of soil ulmin, C/N: the method by regulation in " soil physical analysis " (Shanghai science tech publishing house, 1978) is carried out.
Ocular estimate method: undertaken by the method for stipulating among " Shanghai environmental science " calendar year 2001 the 20th volume the 2nd phase P85-87.
Temperature variation curve contrast situation is seen Fig. 1, and soil ulmin changes the contrast situation and sees Fig. 2; Fig. 3 is seen in the C/N variation in the composting process.
Other relevant evaluation situations, for example the compost ocular estimate result such as the following subordinate list of different fermentations agent production are put down in writing:
| Starter | Ocular estimate |
| Contrast | Chocolate; Frowziness, no humic breath; Attract a small amount of mosquitos and flies; Particle diameter is than the people, and the part caking is clamminess, and feel is thicker, gets 2 fens. |
| A | Chocolate: no stink, significantly humic breath; Do not attract mosquitos and flies; The crumb structure that drought is loose, feel is thinner: place one day rear surface adularescent mycelial growth, got 9 fens. |
| B | Chocolate; Slightly frowziness has the slight humic breath that gets; Do not attract mosquitos and flies: apparent more loose, a small amount of caking, feel is thick slightly, gets 5 fens. |
| C | Chocolate; Slight stink, significantly humic breath; Do not attract mosquitos and flies: apparent more loose, the minute quantity caking; Placing two days rear surfaces has a small amount of mycelia, gets 7 fens. |
(4) starter comparing result
A, heat up from compost fermentation, starter A can reach 54 ℃ in second day, and than other several starters fast 1-3 days, high temperature (more than 60 ℃) can be kept 9 days, than other several starters long 2-5 days.
B, through behind 16 days compost fermentations, from the content of humic substances increase, starter A is increased to 17.7% from 1.9% of beginning, effect is best.Be followed successively by starter C＞starter B＞contrast.
C, from C/N, contrast and starter B, compost is after 16 days, C/N dropped to 20.9: 1 respectively and 20.2: 1 from 28: 1, used starter A, C, compost is after 16 days, from 16.5: 1 and 16.9: 1 of decline in 28: 1, effect was more satisfactory respectively for C/N.
D, from appearance index, the compost that starter A produces got 9 fens, the compost that starter C produces got 7 fens, the compost that starter B produces got 5 fens, contrast 2 minutes.
In sum: the present invention's starter that becomes thoroughly decomposed is compared with the starter of prior art, and comprehensive treatment capability is much better than, and effect is much better.
Method and technology effect of the present invention is, technology is simple, cost is low, constant product quality, to provide a kind of fermentation to play temperature fast for the comprehensive treating process of organic liquid waste waste residue and utilization, and the cycle is short, and abilities such as decomposition of cellulose, hemicellulose, xylogen, protein are strong, the material degree height that becomes thoroughly decomposed, the production method that fermenting process is not smelly.
Claims (10)
1. be used for the industrial organic waste liquid waste residue and handle and the starter that becomes thoroughly decomposed that utilizes, it is characterized in that it is combined by one of cellulose-decomposing bacterium, hemicellulose decomposer, protein decomposing bacteria, these bacterium bacterial classification kind songs of amylolysis bacterium or its; Described bacterial classification preferentially is selected from following bacterial classification or their combination: aspergillus niger (Aspergillus niger), viride (Trichoderma viride), the button capsule is intended spore mould (Endomycopsis fibnliger), subtilis (Bacillus subtilis), cereuisiae fermentum (Saccharomyces cerevisiae); The bacterial classification capacity of decomposition is one of the following or respective combination: live 50～1000 milligrams of glucose/grams hour of CMC enzyme, 500～5000 milligrams of wood sugar/grams of xylanase activity hour, live 50～500 micrograms tyrosine/gram hour of neutral protease, 100～1000 milligrams of glucose/grams of saccharifying enzyme work hour.
2. the starter that becomes thoroughly decomposed according to claim 1 is characterized in that, described bacterial classification is an aspergillus niger, viride, and it is mould that the button capsule is intended spore, subtilis, the arbitrary single bacterial strain of cereuisiae fermentum is formed.
3. the starter that becomes thoroughly decomposed according to claim 1 is characterized in that, it is mould that it intends spore by the button capsule, and viride is formed, live 1000 milligrams of glucose/grams hour of its CMC enzyme, saccharifying enzyme 500 milligrams of glucose/grams alive hour.
4. the starter that becomes thoroughly decomposed according to claim 1 is characterized in that, it is mould that it intends spore by the button capsule, and subtilis is formed, live 1000 milligrams of glucose/grams hour of its saccharifying enzyme, neutral protease 500 micrograms tyrosine alive/gram hour.
5. the starter that becomes thoroughly decomposed according to claim 1 is characterized in that, it is by Endomycopsis Fibnligera, subtilis, and aspergillus niger is formed, and its neutral protease activity 500 micrograms tyrosine/gram hour, xylanase activity are 5000 milligrams of wood sugar/grams hour.
6. the starter that becomes thoroughly decomposed according to claim 1, it is characterized in that, it is by aspergillus niger, viride, and it is mould that the button capsule is intended spore, subtilis, cereuisiae fermentum is formed, and its CMC enzyme work is 50 milligrams of glucose/grams hour, and xylanase activity is 500 milligrams of wood sugar/grams hour, live 100 milligrams of glucose/grams hour of saccharifying enzyme, neutral protease work 50 micrograms tyrosine/gram hour.
7. the starter that becomes thoroughly decomposed according to claim 1, it is characterized in that, it is by aspergillus niger, viride, and it is mould that the button capsule is intended spore, subtilis, cereuisiae fermentum is formed, and its CMC enzyme work is 500 milligrams of glucose/grams hour, and xylanase activity is 2000 milligrams of wood sugar/grams hour, live 800 milligrams of glucose/grams hour of saccharifying enzyme, neutral protease work 250 micrograms tyrosine/gram hour.
8. produce described processing of industrial organic waste liquid waste residue and the starter production method of utilizing of becoming thoroughly decomposed of being used for of claim 1 for one kind, it is characterized in that, at first collect and cultivate the living bacterial strain of cellulase and hemicellulase, in collected bacterial strain, screen highly active decomposition bacterial strain then, composition and technical parameter according to the starter that becomes thoroughly decomposed requires to be mixed with the starter that becomes thoroughly decomposed at last, and it comprises the steps:
1), produce the bacterial classification song:
(1) obtain three-class strain: with first class inoculum is the test tube kind, through the processing of switching enlarged culturing, obtains second class inoculum, i.e. triangular flask solid kind, then with second class inoculum through tortuous or plant bent machine enlarged culturing and obtain three-class strain, promptly produce kind;
(2) will be that the substratum of main raw material(s) adds water with wheat bran, the sterilization of goal Guo Bamboo vapour be placed on spreading for cooling on the Ventilated bed then;
(3) inoculation: the production kind of above-mentioned the first step acquisition is inoculated on the sterilization raw material of spreading for cooling, ventilated and cultivate, obtain kind of a song;
(4) check: purebred song is tested, and wherein, water content is 25～50%;
2) prepare the starter that becomes thoroughly decomposed:
(1) step of the starter that becomes thoroughly decomposed of promptly using of preparation wet type is: with the above-mentioned the 1st) each obtained purebred song of step, mix by predetermined required bacterial classification combination requirement, promptly obtain the aqueous i.e. starter that becomes thoroughly decomposed of usefulness of promptly joining;
(2) each obtained purebred Qu Jinhang oven drying at low temperature of the step preparation dry type commodity starter that becomes thoroughly decomposed: with the above-mentioned the 1st) requiredly mixes, dries with bacterial classification combination requirements by predetermined then, i.e. the dried commodity of the acquisition starter that becomes thoroughly decomposed.
9. the production method of the starter that becomes thoroughly decomposed according to claim 8 is characterized in that, bacterial classification is an aspergillus niger, viride, it is mould that the button capsule is intended spore, subtilis, and the arbitrary single bacterial strain of cereuisiae fermentum, substratum is: wheat bran 50%, dregs of beans 10%, straw powder 40%, material-water ratio are 1: 1; Ventilate and cultivated 2 days, turn over bent 2～3 times, obtain to plant bent; The purebred bent water content that requires set by step to make is 25～40%.
10. the production method of the starter that becomes thoroughly decomposed according to claim 8, it is characterized in that, bacterial classification is an aspergillus niger, viride, and it is mould that the button capsule is intended spore, subtilis, cereuisiae fermentum, its CMC enzyme work are 100 milligrams of glucose/grams hour, and xylanase activity is 500 milligrams of wood sugar/grams hour, live 100 milligrams of glucose/grams hour of saccharifying enzyme, neutral protease work 50 micrograms tyrosine/gram hour; Substratum is: wheat bran 50%, corn cob 50%; Material-water ratio is 1: 1; Ventilate and cultivated 3 days, turn over bent 3～5 times, obtain to plant bent; The purebred bent water content that requires set by step to make is 40～50%.
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Assignee: WUHAN KERNEL BIO-TECH Co.,Ltd. Assignor: Huazhong Agricultural University Contract record no.: 2011420000049 Denomination of invention: Decomposed leaven for the treatment and use of industrial waste liquid and waste residue and method for preparing the same Granted publication date: 20060315 License type: Exclusive License Open date: 20041117 Record date: 20110418 |
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