CN106699416A - Multielement liquid compound microorganism fertilizer and preparation method thereof - Google Patents
Multielement liquid compound microorganism fertilizer and preparation method thereof Download PDFInfo
- Publication number
- CN106699416A CN106699416A CN201710100820.1A CN201710100820A CN106699416A CN 106699416 A CN106699416 A CN 106699416A CN 201710100820 A CN201710100820 A CN 201710100820A CN 106699416 A CN106699416 A CN 106699416A
- Authority
- CN
- China
- Prior art keywords
- fertilizer
- high density
- bacterial strain
- liquid composite
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Pest Control & Pesticides (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Soil Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a multielement liquid compound microorganism fertilizer and a preparation method thereof and relates to an organic fertilizer containing bacterial culture, mycelia or other analogues and a preparation method thereof. The objective of the invention is to provide the method for preparing the novel multielement liquid compound microorganism fertilizer. According to the method, a high and new synthetic technology is adopted to composite beneficial microorganisms, humic acid (organic substances) and inorganic nutritive elements to form a novel fertilizer. The number of effective living bacteria in the multielement liquid compound microorganism fertilizer is larger than or equal to 0.1 billion /ml. The multielement liquid compound microorganism fertilizer and the preparation method thereof are used for the technical field of agriculture.
Description
Technical field
The present invention relates to agricultural technology field, and in particular to one kind is containing adding bacterial cultures, mycelia or other are similar
Organic fertilizer of thing and preparation method thereof.
Background technology
Growing for crop needs the supply of various nutrients, and the source of nutrient is mainly fertilizer, fertilizer
It is the guarantee of agricultural sustainable development, is the basis of crop yield.In the last century five, sixties, chemical fertilizer and agricultural chemicals
Using making the development of China's agricultural achieve significant progress, while also bringing huge benefit to peasant.But, it is long-term big
Amount uses chemical fertilizer and agricultural chemicals, can cause soil hardening；Soil water holding capacity declines；Fertility is unbalanced in soil, and fertility declines；
Input and output ratio declines.More seriously, these chemical substances can be accumulated in animal and plant body, cause animal-plant material
Noxious material is exceeded, has a strong impact on product quality.At the same time, the ground water regime that the chemical substance of residual passes through soil, it is dirty
Dye water resource, causes irreversible destruction in ecological circulation.In the case, the application of bio-feritlizer turns into agricultural development
In focus.Wherein microbial manure is a kind of new-type fertilizer that recent decades grow up.
Microbial manure refers to the particular product containing active microorganism.The major advantage of microbial manure is can conditioned soil
Earth, it is free from environmental pollution, have no toxic side effect, be produce " pollution-free food " preferable fertilizer.Microbial manure is applied to production, can
Promote soil fertility, assist plant absorption nutrition, strengthen plant disease-resistant and drought-resistant ability, energy saving reduces production cost, subtracts
Few environmental pollution.
The country has been achieved for certain progress for the research of microbial manure at present.But, the subject matter for existing
It is also the key for restricting microbial manure development.Mainly have it is following some：One is that the strain that microbial-bacterial fertilizer is used is single, and it should
It is restricted with effect.Two is that nutritive element content is unbalanced in microbial manure, constrains microbial-bacterial fertilizer and promotes on a large scale.
Three be microbial-bacterial fertilizer production technology management and control it is not tight, cause miscellaneous bacteria rate higher, practical application effect is undesirable.Four is microorganism
The market confusion of bacterial manure, mixes the genuine with the fictitious, product quality it cannot be guaranteed that.
Therefore, it is necessary to develop a kind of polynary liquid composite microbiological fertilizer, the product can provide polynary microorganism
Effect, moreover it is possible to which nutrient in a balanced way is provided, and its production technology management and control is simple and direct is easy to enterprise managing product quality.
The content of the invention
It is an object of the invention to provide a kind of preparation method of new multicomponent liquid composite microbe fertilizer, the method is used
A kind of new fertilizer that be composited for beneficial microbe, humic acid (organic substance) and inorganic nutritive element by high-new synthetic technology
Material.The product can provide effect of polynary microorganism, moreover it is possible to provide nutrient in a balanced way, and its production technology management and control letter
Enterprise managing product quality is easy in victory.
The present invention relates to a kind of polynary liquid composite microbiological fertilizer, the fertilizer includes：Complex microorganism, a large amount of nutrition
Element, Meso- and micro-nutrients and humic acid；
Complex microorganism includes bacillus megaterium, bacillus licheniformis and streptomyces microflavus；
Living bacteria count >=1.0 hundred million/mL in the fertilizer；
The macronutrient includes：Nitrogen, P elements and potassium element；
The Meso- and micro-nutrients include：Manganese element, boron element, Zn-ef ficiency.
Preferably, a great number of elements in the fertilizer is by ammonium sulfate (analysis is pure), potassium dihydrogen phosphate (analysis is pure), potassium sulfate
(analysis is pure), diammonium hydrogen phosphate (analysis is pure) and urea (analysis is pure) are provided.
Preferably, (N+P in the fertilizer2O5+K2O) >=200g/L, it is preferred that N+P2O5+K2O ﹕ 8-8-4 (S).
Above-mentioned N+P2O5+K2O ﹕ 8-8-4 (S) refer to the N containing 80g/L in polynary liquid composite microbiological fertilizer,
The P of 80g/L2O5, 40g/L K2O, and the nutritive salt of above-mentioned addition is formulated with the salt of sulfate ion, without
It is that addition chlorion salt is formulated, so, like sulphion for some, belong to excellent for the plant sensitive to chlorion
Select fertilizer.
Preferably, the Meso- and micro-nutrients in the fertilizer are by manganese sulfate (analysis is pure), boric acid (analysis is pure) and sulfuric acid
Zinc (analysis is pure) is provided, and Mn+Zn+B >=4g/L in the fertilizer.
Preferably, in the fertilizer humic acid amount >=30g/L.
Bacillus megaterium (Bacillus is with the addition of in polynary liquid composite microbe fertilizer of the invention
Megaterium), bacillus licheniformis (Bacilluslicheniformis) and streptomyces microflavus (Streptomyces
Microflavus), effect of these three strains is as follows：
Bacillus megaterium belongs to bacillus.It can form gemma, and the capability of resistance to radiation of its gemma is large intestine bar
36 times of bacterium.It is commercially used for producing glucose isomerase, while be also the decomposer of organophosphor, therefore, agriculturally can use
In manufacture phosphorous bacterial fertilizer.Using crop：Ginger, orchid；Controlling object：Ginger bacterial wilt, cymbidium anthracnose disease.
Bacillus licheniformis can produce antibacterial substance, kill pathogenic bacteria, and take oxygen effect machine by force with unique biology
System, can suppress the growth and breeding of pathogenic bacteria, there is promotion growth to beneficial bacterium.Bacillus licheniformis can produce various active
Enzyme, such as protease, amylase, lipase, pectase, dextranase, cellulase.Bacillus licheniformis is in certain bar simultaneously
Resistance endospore can be produced under part, lipopeptid class, peptides, phospholipid, many alkenes and amino acids etc. can be produced various anti-
Various pathogens are played good inhibiting effect by raw element.Bacillus licheniformis can produce various nutriments such as to tie up life simultaneously
Element, amino acid, organic acid, somatomedin etc., for plant provides nutriment.
Streptomyces microflavus are streptomyces actinomyces.Contain auxin, antibiotic, phenylacetic acid, amber in its metabolite
The growth regulator composition necessary to plant growth such as acid and the basic element of cell division, with conversion Nitrogen In Soils, phosphorus, potassium, improves soil
Earth fertility, reduces fertilizer amount；Suppress germ reproduction, diseases prevention is kept a full stand of seedings；Stimulate cell division, promote crop take root, germinate, into
It is ripe, improve crop yield；Promote active principle synthesis, significantly improve the effect such as quality of agricultural product, reach " increasing both production and income, guarantor
Water support field, suppress disease, improve quality " quadruple effect unification effect.
Bacillus megaterium, the three kinds of strains of bacillus licheniformis and streptomyces microflavus added in the present invention, belong to
Exempting from the middle of NY/T 1109-2006 microbial manure bio-safety current techique criterions makees toxicology test strain, also, often
A kind of bacterial strain has all been widely used in the middle of the production of bacteria agent, the invention reside in by effect set of above-mentioned three kinds of strains
Together, many effect purposes of product are realized.
The invention further relates to a kind of preparation method of polynary liquid composite microbiological fertilizer, methods described includes：
(1) the bacterial strain activation of bacillus megaterium, bacillus licheniformis and streptomyces microflavus and bacterial strain High Density Cultivation, point
Streptomyces microflavus high density bacterium solution, bacillus licheniformis high density bacterium solution and Bacillus megatherium high density bacterium solution are not obtained；
(2) NKP macronutrients are allocated, NKP macronutrient solution is obtained；
(3) Meso- and micro-nutrients are allocated, Meso- and micro-nutrients solution is obtained；
(4) humic acid is allocated：By obtained NKP macronutrients solution in humic acid and above-mentioned steps, in micro battalion
Support Element Solution, streptomyces microflavus high density bacterium solution, bacillus licheniformis high density bacterium and Bacillus megatherium high density bacterium solution
It is well mixed, that is, polynary liquid composite microbiological fertilizer is obtained.
Main processes of the invention include bacterial strain activate and bacterial strain High Density Cultivation, allotment NKP macronutrients,
Allotment Meso- and micro-nutrients and the allotment big process of humic acid four, mainly by Intermittent fermentation technology (seed spreads cultivation) and in batches
Mixing technology (in batches dissolve) is combined, thus ensure product and meanwhile have high density living bacteria count, multielement nutrition element with
And high-concentration raw active substances.
Preferably, in the step (1) bacillus megaterium, bacillus licheniformis and streptomyces microflavus bacterial strain activation and
Bacterial strain High Density Cultivation is comprised the following steps：
1. bacterial strain activation and fermentation medium are prepared：Potassium dihydrogen phosphate (chemistry is pure) 5g ± 0.1g, magnesium sulfate are weighed respectively
(chemistry is pure) 0.5g ± 0.05g, sodium chloride (chemistry is pure) 5g ± 0.1g, glucose (chemistry is pure) 10g ± 0.1g, peptone are (raw
Change reagent) 10g ± 0.1g, yeast extract (biochemical reagents) 4g ± 0.1g and Tween-80 (chemistry is pure) 3g ± 0.1g, it is subsequently adding
1000mL ± 10mL purified waters (by life water after 1 μm of pp filter cores and extruded activated carbon filter element filtering), after stirring,
Under conditions of 110 DEG C~121 DEG C, sterilize 20min~60min, is cooled to standby after room temperature；
2. bacterial strain activation：Aseptically, the purifying bacterial strain deposited using ring~5 environmental protection of oese picking 3, is inoculated in
In 100mL culture mediums (300mL triangular flasks), shaking flask activation culture (first order seed culture) is carried out, condition of culture is：Cultivation temperature
28 DEG C~37 DEG C, rotating speed 160r/min~200r/min, pH value 6.2~7.5 terminates activation, and sample after culture 16h~24h
Microscopy, analyzes thalline quantity and microbiological contamination situation.The one-level bacterial strain that will have been activated is in 2wt%~10wt% ratios (for being inoculated with
Level bacterial strain accounts for the total amount 2wt%~10wt% of inoculation after fermentation liquid), being inoculated in 300mL culture mediums (1000mL triangular flasks) is carried out
Bacterial strain Amplification Culture (secondary seed culture), condition of culture ibid, after culture 16h~24h, terminates bacterial strain Amplification Culture, and take
Sample microscopy, analyzes thalline quantity and microbiological contamination situation；
3. bacterial strain High Density Cultivation：The two grades of bacterial strains that will have been activated are inoculated in fermentation tank in 5wt%~10wt% ratios
The High Density Cultivation of bacterial strain is carried out, condition of culture is：28 DEG C~37 DEG C of cultivation temperature, speed of agitator 160r/min~200r/
Min, throughput 1.0vvm~1.5vvm, pH value 6.2~7.5 terminate fermentation, and zymotic fluid is stored in after culture 20h~60h
It is standby in 4 DEG C of refrigerating boxes.And using colony counting method to zymotic fluid in effective bacterium number be analyzed, take 1mL zymotic fluids dilution
10-5~10-8After times, at 28 DEG C~37 DEG C, after solid culture 18h~48h, the clump count on flat board is counted, it is ensured that
Living bacteria count is 5.0 × 10 in zymotic fluid7CFU/mL~2.0 × 109Within the scope of CFU/mL.
Bacterial strain in the present invention in bacillus megaterium, bacillus licheniformis and streptomyces microflavus is activated and bacterial strain high density
In incubation, the formula of the activation fermentation mediums of three kinds of strains is identical, activation fermentation process parameter control scope is consistent,
The saving dispensing time is advantageous in that, facilitates technical staff to manage control, save cost of labor.
Preferably, allotment NKP macronutrients are comprised the following steps in the step (2)：
As a example by preparing many first liquid composite microbiological fertilizers of 1L, the concocting method of NKP macronutrients is as follows：It is first
First, ammonium sulfate (analysis is pure) 80g~85g, potassium dihydrogen phosphate (analysis is pure) 85g~90g, potassium sulfate are weighed respectively (analysis is pure)
20g~25g, diammonium hydrogen phosphate (analysis pure) 80g~85g and urea (analysis is pure) 110g~120g, be subsequently adding 600mL~
650mL purified waters, water temperature, with 300r/min~400r/min rotating speeds, stirs 15min~40min, to reagent at 45 DEG C~60 DEG C
All dissolvings, liquor capacity increases to 800mL~850mL, solution ph 5.0~6.5, (N+P2O5+K2O) >=200g/L, N+
P2O5+K2O ﹕ 8-8-4 (S), allotment completes standby.
Preferably, allotment Meso- and micro-nutrients are comprised the following steps in the step (3)：
As a example by preparing many first liquid composite microbiological fertilizers of 1L, the concocting method of Meso- and micro-nutrients is as follows：First,
Manganese sulfate (analysis is pure) 2g~2.5g, boric acid (analysis is pure) 2g~2.5g and zinc sulfate (analysis is pure) 10g~11g are weighed respectively,
60mL~62mL purified waters are subsequently adding, water temperature, with 300r/min~400r/min rotating speeds, stirs 10min at 20 DEG C~30 DEG C
~15min, is all dissolved to reagent, and liquor capacity increases to 65mL~67mL, and pH value is adjusted in 4.5~5.0, Mn+Zn+B >=4g/L
It is standby with completing.
Preferably, allotment humic acid is comprised the following steps in the step (4)：
As a example by preparing many first liquid composite microbiological fertilizers of 1L, the concocting method of humic acid is as follows：First, weigh respectively
Humic acid (chemistry pure) 40g~45g, be then slowly added to successively NKP macronutrients solution 800mL~850mL, in it is micro
Nutrient solution 65mL~67mL, streptomyces microflavus high density bacterium solution 20mL~25mL, bacillus licheniformis high density bacterium solution
40mL~45mL, Bacillus megatherium high density bacterium solution 40mL~45mL and appropriate purified water to overall solution volume reach
1000mL~1050mL, afterwards, then adds appropriate NaOH (analysis pure) pulvis, makes pH value in the range of 5.5~6.5.Most
Afterwards, at room temperature, with 300r/min~400r/min rotating speeds, 40min~60min is stirred, humic acid >=30g/L reaches fully
Mixing and quick formation humic acid chelate purpose.
The technical indicator of obtained product (with many first liquid composite microbiological fertilizers of the 1L for being prepared) according to the method described above
It is as follows：
1st, NKP macronutrients：(N+P2O5+K2O) >=200g/L, N+P2O5+K2O ﹕ 8-8-4 (S)；
2nd, micronutrient element：Mn+Zn+B≥4g/L；
3rd, humic acid >=30g/L；
4th, pH value 5.5~6.5；
5th, living bacteria count >=1.0 hundred million/mL.
The purpose and meaning of concrete operations of the present invention：
(1) raw material used in the present invention need to be the pure and mild biochemical reagents grade reagents of analysis, and allotment water need to be through 1 μm of pp
Used after filter core and extruded activated carbon filter element filtering, it is therefore an objective to reduce fertilizer poisonous and harmful substance intake, also help reduction
The miscellaneous bacteria rate of fertilizer.
(2) in the present invention bacterial strain High Density Cultivation technology can realize the living bacteria count of bacillus megaterium 1.5 ×
109CFU/mL~2.0 × 109CFU/mL, the living bacteria count of bacillus licheniformis is 1.5 × 109CFU/mL~2.0 ×
109CFU/mL, the living bacteria count of streptomyces microflavus is 5.0 × 107CFU/mL~6.0 × 107CFU/mL。
(3), it is necessary to add streptomyces microflavus high density bacterium when preparing many first liquid composite microbiological fertilizers of 1L in the present invention
Liquid 20mL~25mL, bacillus licheniformis high density bacterium solution 40mL~45mL and Bacillus megatherium high density bacterium solution 40mL~
45mL, it is therefore an objective to ensure the living bacteria count >=1.0 hundred million/mL in polynary liquid composite microbiological fertilizer.
(4) present invention in mixed bacteria liquid adding proportion >=10%, it is therefore an objective to ensure polynary liquid composite microbiological fertilizer
In, contain various active enzyme, such as protease, amylase, lipase, pectase, dextranase, cellulase；Also containing various
Growth regulator, such as antibiotic, auxin, antibiotic, phenylacetic acid, butanedioic acid, bacteriocin and the basic element of cell division；And various battalion
Support material, such as vitamin, amino acid, organic acid, somatomedin material.
(5) in the present invention using high-purity sulfuric acid root class nutritive salt (analysis pure) as compounding raw material, its benefit be reduction arsenic,
Cadmium, lead, chromium, the addition of mercury harmful element simultaneously realize the exploitation that fertilizer product is added without chlorion.
Polynary liquid composite microbiological fertilizer of the invention using high-new synthetic technology by beneficial microbe, organic substance and
A kind of new-type fertilizer that inorganic nutritive element is composited.Its feature is as follows：1st, can to plant growth provide needed for NPK and
Middle trace element, moreover it is possible to for crop provides organic matter and beneficial microbe active bacteria.2nd, soil beneficial microbe bacterium can be increased
Quantity, realize chesson, Soluble phosphorus potassium decomposing, effect of culture fertility.3rd, after microbial bacteria is bred in soil, produce a large amount of
Plant hormone and organic acid, stimulate root growth and development, strengthen the rate of photosynthisis of crops, reach strong plantlets and rootage, reduce sub-
Nitrate content, improve quality realizes increasing both production and income.4th, microbial bacteria can speed up the degraded of soil with organic matter, be farming
Thing growth provides more organic nutrient substances, after microbial bacteria breeding, secretes substantial amounts of chitinase, ectoenzyme and antibiotic etc.
Material, can effectively crack the conidial cell wall of harmful fungoid, and the growth of line eggs wall and suppression harmful bacteria efficiently controls native transmissibility
The generation of pest and disease damage, plays effect of diseases prevention insect prevention and anti-continuous cropping.
The inventive method has the following advantages that：
1st, its total nutrient of the product (N+P prepared by the present invention2O5+K2O) >=200g/L, N+P2O5+K2O ﹕ 8-8-4 (S),
Mn+Zn+B >=4g/L, humic acid >=30g/L.The beneficial effect of this product is many needed for containing abundant organic matter and plant
Nutrient is planted, nutrient content is high, fertilizer efficiency is fast, obvious effect of increasing production.
2nd, product its living bacteria count >=1.0 hundred million/mL prepared by the present invention, improves soil beneficial microbe bacterium
Quantity, realizes chesson, and Soluble phosphorus potassium decomposing, culture fertility, improvement soil texture, raising soil nutrient capacity, enhancing soil are protected
Fertile water holding capacity, regulation soil acidity or alkalinity, promotion soil microbial activity, raising soil nutrient availability, the corresponding nutrition of reduction
The dose of element.
3rd, the microbial bacteria prepared by the present invention can produce substantial amounts of plant hormone and organic acid, stimulate root growth to send out
Educate, strengthen the rate of photosynthisis of crops, reach strong plantlets and rootage, reduce content of nitrite, improve quality realizes increasing both production and income.
4th, the microbial bacteria prepared by the present invention can speed up the degraded of soil with organic matter, for crop growth is provided more
Many organic nutrient substances, after microbial bacteria breeding, secrete the materials, Ke Yiyou such as substantial amounts of chitinase, ectoenzyme and antibiotic
The conidial cell wall of effect cracking harmful fungoid, the growth of line eggs wall and suppression harmful bacteria efficiently controls the hair of native transmissibility pest and disease damage
It is raw, play effect of diseases prevention insect prevention and anti-continuous cropping.
Brief description of the drawings
Fig. 1 is the flow chart of the inventive method.
Specific embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.
Embodiment 1
As shown in figure 1, the polynary liquid composite microbiological fertilizer of the present embodiment is prepared according to the following steps：
(1) the step of High Density Cultivation of bacillus megaterium：
Aseptically, the bacillus megaterium deposited using the environmental protection of oese picking 5 is inoculated in 100mL culture mediums
In (300mL triangular flasks), shake flask fermentation culture (first order seed culture) is carried out, condition of culture is：30 DEG C of cultivation temperature, during fermentation
Between 20h, rotating speed 180r/min, pH value 6.5.By above-mentioned cultured first order seed, 2L culture mediums are inoculated in 5% ratio
In (3.7L fermentation tanks), the fermented and cultured of bacillus megaterium is carried out, condition of culture is：30 DEG C of fermentation temperature, throughput
1.0vvm, rotating speed 180r/min, pH value 6.5, fermentation time 20h after fermentation ends, zymotic fluid are stored in standby in 4 DEG C of refrigerators
With.Colony counting method is used, 1mL zymotic fluids is taken and is diluted 10-8After times, at 30 DEG C, after culture 24h, on flat board
Bacterium colony counted, living bacteria count 1.5 × 10 in zymotic fluid9CFU/mL~2.0 × 109CFU/mL。
The composition of seed and fermentation medium includes：Potassium dihydrogen phosphate (chemistry is pure) 5g, magnesium sulfate (chemistry is pure) 0.5g, chlorine
Change sodium (chemistry is pure) 5g, glucose (chemistry is pure) 10g, peptone (biochemical reagents) 10g, yeast extract (biochemical reagents) 4g and tell
Temperature -80 (chemistry is pure) 3g, is subsequently adding 1000mL purified waters, and after stirring, under the conditions of 121 DEG C, sterilize 20min, cooling
It is standby after to room temperature.
(2) the step of High Density Cultivation of bacillus licheniformis：
Aseptically, the bacillus licheniformis deposited using the environmental protection of oese picking 5 is inoculated in 100mL culture mediums
In (300mL triangular flasks), shake flask fermentation culture (first order seed culture, i.e. first class inoculum culture) is carried out, condition of culture is：Culture
30 DEG C of temperature, fermentation time 20h, rotating speed 180r/min, pH value 6.5.By above-mentioned cultured first order seed, connect in 5% ratio
Plant in 2L culture mediums (3.7L fermentation tanks), carry out the fermented and cultured of bacillus licheniformis, condition of culture is：Fermentation temperature 30
DEG C, zymotic fluid after fermentation ends, is stored in 4 DEG C by throughput 1.5vvm, rotating speed 160r/min, pH value 6.5, fermentation time 20h
It is standby in refrigerator.Colony counting method is used, 1mL zymotic fluids is taken and is diluted 10-8After times, at 30 DEG C, after culture 24h,
Bacterium colony on flat board is counted, living bacteria count 1.5 × 10 in zymotic fluid9CFU/mL~2.0 × 109CFU/mL。
The composition of seed and fermentation medium includes：Potassium dihydrogen phosphate (chemistry is pure) 5g, magnesium sulfate (chemistry is pure) 0.5g, chlorine
Change sodium (chemistry is pure) 5g, glucose (chemistry is pure) 10g, peptone (biochemical reagents) 10g, yeast extract (biochemical reagents) 4g and tell
Temperature -80 (chemistry is pure) 3g, is subsequently adding 1000mL purified waters, and after stirring, under the conditions of 121 DEG C, sterilize 20min, cooling
It is standby after to room temperature.
(3) the step of High Density Cultivation of streptomyces microflavus：
Aseptically, the streptomyces microflavus deposited using the environmental protection of oese picking 5 are inoculated in 100mL culture mediums (300mL
Triangular flask) in, shake flask fermentation culture (first order seed culture) is carried out, condition of culture is：30 DEG C of cultivation temperature, fermentation time 20h,
Rotating speed 180r/min, pH value 6.5.By above-mentioned cultured first order seed, (3.7L ferments to be inoculated in 2L culture mediums in 5% ratio
Tank) in, the fermented and cultured of streptomyces microflavus is carried out, condition of culture is：30 DEG C of fermentation temperature, throughput 1.5vvm, rotating speed 180r/
Min, pH value 6.5, fermentation time 60h after fermentation ends, zymotic fluid is stored in standby in 4 DEG C of refrigerators.Use flat board meter
Number method, takes 1mL zymotic fluids and is diluted 10-6After times, at 30 DEG C, after culture 48h, the bacterium colony on flat board is counted, sent out
Living bacteria count 5.0 × 10 in zymotic fluid7CFU/mL~6.0 × 107CFU/mL。
The composition of seed and fermentation medium includes：Potassium dihydrogen phosphate (chemistry is pure) 5g, magnesium sulfate (chemistry is pure) 0.5g, chlorine
Change sodium (chemistry is pure) 5g, glucose (chemistry is pure) 10g, peptone (biochemical reagents) 10g, yeast extract (biochemical reagents) 4g and tell
Temperature -80 (chemistry is pure) 3g, is subsequently adding 1000mL purified waters, and after stirring, under the conditions of 121 DEG C, sterilize 20min, cooling
It is standby after to room temperature.
(4) adaptation step of NKP macronutrients：(preparing many first liquid composite microbiological fertilizers of 1L)
Ammonium sulfate (analysis is pure) 80g, potassium dihydrogen phosphate (analysis is pure) 85g, potassium sulfate (analysis is pure) 20g, phosphorus are weighed respectively
The ammonium of sour hydrogen two (analysis pure) 80g and urea (analysis is pure) 110g, is subsequently adding 600mL purified waters, water temperature at 45 DEG C, with 400r/
Min rotating speeds, stir 20min, and reagent all dissolves, and liquor capacity increases to 790mL, pH value 6.0~6.5, (N+P2O5+K2O)≥
200g/L, N+P2O5+K2O ﹕ 8-8-4 (S), allotment completes standby.
(5) the step of allotment of Meso- and micro-nutrients (preparing many first liquid composite microbiological fertilizers of 1L)
Manganese sulfate (analysis is pure) 2g, boric acid (analysis is pure) 2g and zinc sulfate (analysis is pure) 10g are weighed respectively, are subsequently adding
60mL purified waters, water temperature, with 400r/min rotating speeds, stirs 10min at 30 DEG C or so, and reagent is all dissolved, and liquor capacity is increased to
65mL, pH value completes standby in 4.5~5.0, Mn+Zn+B >=4g/L, allotment.
(6) the step of allotment of humic acid：(preparing many first liquid composite microbiological fertilizers of 1L)
First, weigh humic acid (chemistry pure) 40g, and be slowly added to successively NKP macronutrient solution 790mL, in
It is micronutrient element solution 65mL, streptomyces microflavus high density bacterium solution 20mL, bacillus licheniformis high density bacterium solution 40mL, huge
Bacillus high density bacterium solution 40mL and 40mL purified water makes overall solution volume reach 1000mL.And at room temperature, with 300r/
Min rotating speeds, stir 50min, and regulation pH value is 6.2, that is, polynary liquid composite microbiological fertilizer, polynary liquid composite microbial is obtained
Content of humic acid >=30g/L in thing fertilizer.
Embodiment 2
The polynary liquid composite microbiological fertilizer and the difference of embodiment 1 of the present embodiment be：
The composition of seed and fermentation medium includes in step (1)：Potassium dihydrogen phosphate (chemistry is pure) 4.9g, magnesium sulfate (are changed
Learn pure) 0.45g, sodium chloride (chemistry pure) 4.9g, glucose (chemistry is pure) 9.9g, peptone (biochemical reagents) 9.9g, yeast leaching
Powder (biochemical reagents) 3.9g and Tween-80 (chemistry is pure) 2.9g, is subsequently adding 990mL purified waters, after stirring, at 110 DEG C
Under the conditions of, sterilize 40min.
The composition of seed and fermentation medium includes in step (2)：Potassium dihydrogen phosphate (chemistry is pure) 5.1g, magnesium sulfate (are changed
Learn pure) 0.55g, sodium chloride (chemistry pure) 5.1g, glucose (chemistry is pure) 10.1g, peptone (biochemical reagents) 10.1g, yeast
Leaching powder (biochemical reagents) 4.1g and Tween-80 (chemistry is pure) 3.1g, is subsequently adding 1010mL purified waters, after stirring, 115
Under the conditions of DEG C, sterilize 60min.
The composition of seed and fermentation medium includes in step (3)：Potassium dihydrogen phosphate (chemistry is pure) 5.1g, magnesium sulfate (are changed
Learn pure) 0.55g, sodium chloride (chemistry pure) 5.1g, glucose (chemistry is pure) 10.1g, peptone (biochemical reagents) 10.1g, yeast
Leaching powder (biochemical reagents) 4.1g and Tween-80 (chemistry is pure) 3.1g, is subsequently adding 1010mL purified waters, after stirring, 116
Under the conditions of DEG C, sterilize 40min.
Embodiment 3
The polynary liquid composite microbiological fertilizer and the difference of embodiment 2 of the present embodiment be：
The allotment of NKP macronutrients is carried out according to the following steps in step (4)：
Ammonium sulfate (analysis is pure) 85g, potassium dihydrogen phosphate (analysis is pure) 90g, potassium sulfate (analysis is pure) 25g, phosphorus are weighed respectively
The ammonium of sour hydrogen two (analysis pure) 85g and urea (analysis is pure) 120g, is subsequently adding 650mL purified waters, water temperature at 60 DEG C, with 300r/
Min rotating speeds, stir 15min, reagent all dissolve, pH value 5.0~6.0, (N+P2O5+K2O) >=200g/L, N+P2O5+K2O﹕
8-8-4 (S), allotment completes standby.
Embodiment 4
The polynary liquid composite microbiological fertilizer and the difference of embodiment 3 of the present embodiment be：
The allotment of step (5) Meso- and micro-nutrients is carried out according to the following steps：
Manganese sulfate (analysis is pure) 2.5g, boric acid (analysis is pure) 2.5g and zinc sulfate (analysis is pure) 11g, Ran Houjia are weighed respectively
Enter 62mL purified waters, water temperature with 300r/min rotating speeds, stirs 15min at 20 DEG C, and reagent all dissolves, pH value 4.5~5.0,
Mn+Zn+B >=4g/L, allotment completes standby.
Embodiment 5
The polynary liquid composite microbiological fertilizer and the difference of embodiment 1 of the present embodiment be：
The allotment of step (6) humic acid is carried out according to the following steps：
First, weigh humic acid (chemistry pure) 45g, and be slowly added to successively NKP macronutrient solution 850mL, in
It is micronutrient element solution 67mL, streptomyces microflavus high density bacterium solution 25mL, bacillus licheniformis high density bacterium solution 45mL, huge
Bacillus high density bacterium solution 45mL, being subsequently adding purified water makes overall solution volume reach 1050mL.And at room temperature, with
400r/min rotating speeds, stir 40min, and regulation pH value is 5.5, that is, polynary liquid composite microbiological fertilizer is obtained, and polynary liquid is answered
Close content of humic acid >=30g/L in microbial manure.
The culture presevation used in above-described embodiment is thin yellow in Chinese industrial Microbiological Culture Collection administrative center (CICC)
Streptomycete (Streptomycesmicroflavus), deposit number is CICC11006；Bacillus licheniformis
(Bacilluslicheniformis), deposit number is CICC10092；Bacillus megatherium (Bacillusmegaterium),
Deposit number is CICC20665.
The requirement of the reagent, raw material and the water that are used in above-described embodiment：Diammonium phosphate (DAP) (analysis pure), urea (analysis is pure),
Ammonium sulfate (analysis pure), potassium dihydrogen phosphate (analysis is pure), potassium sulfate (analysis is pure), boric acid (analysis is pure), zinc sulfate (analysis is pure),
Manganese sulfate (analysis pure), humic acid (chemistry is pure), NaOH (analysis is pure), magnesium sulfate (analysis is pure), sodium chloride (analysis is pure),
Glucose (analysis is pure), peptone (biochemical reagents), yeast extract (biochemical reagents), Tween-80 (analysis is pure), purified water (warp
Cross 1 μm pp filter cores and extruded activated carbon filter element filtering after life water).
The many first liquid composite microbiological fertilizers of 1L that above example 1-5 is prepared are detection object, the technology of its product
Index is as follows：
1st, NKP macronutrients：(N+P2O5+K2O) >=200g/L, N+P2O5+K2O ﹕ 8-8-4 (S)；
2nd, micronutrient element：Mn+Zn+B≥4g/L；
3rd, humic acid >=30g/L；
4th, pH value 6.2；
5th, living bacteria count >=1.0 hundred million/mL；
Although the present invention is described in detail above to have used general explanation and specific embodiment, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (10)
1. a kind of polynary liquid composite microbiological fertilizer, it is characterised in that the fertilizer includes：Complex microorganism, a large amount of nutrition
Element, Meso- and micro-nutrients and humic acid；
Complex microorganism includes bacillus megaterium, bacillus licheniformis and streptomyces microflavus；
Living bacteria count >=1.0 hundred million/mL in the fertilizer；
The macronutrient includes：Nitrogen, P elements and potassium element；
The Meso- and micro-nutrients include：Manganese element, boron element, Zn-ef ficiency.
2. polynary liquid composite microbiological fertilizer according to claim 1, it is characterised in that：A large amount of battalion in the fertilizer
Element is supported to be provided by ammonium sulfate, potassium dihydrogen phosphate, potassium sulfate, diammonium hydrogen phosphate and urea.
3. polynary liquid composite microbiological fertilizer according to claim 2, it is characterised in that：(N+P in the fertilizer2O5+
K2O)≥200g/L。
4. polynary liquid composite microbiological fertilizer according to claim 1, it is characterised in that：It is micro in the fertilizer
Nutrient is provided by manganese sulfate, boric acid and zinc sulfate, and Mn+Zn+B >=4g/L in the fertilizer.
5. polynary liquid composite microbiological fertilizer according to claim 1, it is characterised in that：Humic acid in the fertilizer
Amount >=30g/L.
6. a kind of preparation method of polynary liquid composite microbiological fertilizer, it is characterised in that：The described method comprises the following steps：
(1) the bacterial strain activation of bacillus megaterium, bacillus licheniformis and streptomyces microflavus and bacterial strain High Density Cultivation, respectively
To streptomyces microflavus high density bacterium solution, bacillus licheniformis high density bacterium solution and Bacillus megatherium high density bacterium solution；
(2) NKP macronutrients are allocated, NKP macronutrient solution is obtained；
(3) Meso- and micro-nutrients are allocated, Meso- and micro-nutrients solution is obtained；
(4) humic acid is allocated：By obtained NKP macronutrients solution, middle micronutrient unit in humic acid and above-mentioned steps
The mixing of plain solution, streptomyces microflavus high density bacterium solution, bacillus licheniformis high density bacterium and Bacillus megatherium high density bacterium solution
Uniformly, that is, polynary liquid composite microbiological fertilizer is obtained.
7. the preparation method of polynary liquid composite microbiological fertilizer according to claim 6, it is characterised in that：The step
(1) the bacterial strain activation of bacillus megaterium, bacillus licheniformis and streptomyces microflavus and bacterial strain High Density Cultivation include following in
Step：
1. bacterial strain activation and fermentation medium are prepared：Weigh respectively potassium dihydrogen phosphate 5g ± 0.1g, magnesium sulfate 0.5g ± 0.05g,
Sodium chloride 5g ± 0.1g, glucose 10g ± 0.1g, peptone 10g ± 0.1g, yeast extract 4g ± 0.1g and Tween-80 3g ±
0.1g, is subsequently adding 1000mL ± 10mL purified waters, after stirring, under conditions of 110 DEG C~121 DEG C, sterilizing 20min~
60min, is cooled to standby after room temperature；
2. bacterial strain activation：Aseptically, the purifying bacterial strain deposited using ring~5 environmental protection of oese picking 3, is inoculated in 100mL
In culture medium, shaking flask activation culture is carried out, condition of culture is：28 DEG C~37 DEG C of cultivation temperature, rotating speed 160r/min~200r/
Min, pH value is 6.2~7.5, cultivates 16h~24h, obtains the one-level bacterial strain for having activated；The one-level bacterial strain that will have been activated is pressed
2wt%~10wt% ratios, being inoculated in 300mL culture mediums carries out bacterial strain Amplification Culture, and condition of culture is activated with one-level bacterial strain
Condition of culture it is identical, cultivate 16h~24h, obtain the two grades of bacterial strains for having activated；
3. bacterial strain High Density Cultivation：In 5wt%~10wt% ratios, be inoculated in fermentation tank is carried out the two grades of bacterial strains that will have been activated
The High Density Cultivation of bacterial strain, condition of culture is：28 DEG C~37 DEG C of cultivation temperature, speed of agitator 160r/min~200r/min leads to
Tolerance 1.0vvm~1.5vvm, pH value is 6.2~7.5, cultivates 20h~60h, that is, high density bacterium solution is obtained；
The bacterial strain is bacillus megaterium, bacillus licheniformis or streptomyces microflavus.
8. the preparation method of polynary liquid composite microbiological fertilizer according to claim 7, it is characterised in that：The step
(2) allotment NKP macronutrients are comprised the following steps in：
First, ammonium sulfate 80g~85g, potassium dihydrogen phosphate 85g~90g, potassium sulfate 20g~25g, diammonium hydrogen phosphate are weighed respectively
80g~85g and urea 110g~120g；
600mL~650mL purified waters are subsequently adding, water temperature, with 300r/min~400r/min rotating speeds, is stirred at 45 DEG C~60 DEG C
15min~40min, all dissolves to reagent, that is, NKP macronutrient solution is obtained.
9. the preparation method of polynary liquid composite microbiological fertilizer according to claim 8, it is characterised in that：The step
(3) allotment Meso- and micro-nutrients are comprised the following steps in：
First, manganese sulfate 2g~2.5g, boric acid 2g~2.5g and zinc sulfate 10g~11g are weighed respectively；
60mL~62mL purified waters are subsequently adding, water temperature, with 300r/min~400r/min rotating speeds, is stirred at 20 DEG C~30 DEG C
10min~15min, all dissolves to reagent, that is, Meso- and micro-nutrients solution is obtained.
10. the preparation method of polynary liquid composite microbiological fertilizer according to claim 9, it is characterised in that：The step
Suddenly allotment humic acid is comprised the following steps in (4)：
First, weigh humic acid 40g~45g, be then slowly added to successively NKP macronutrients solution 800mL~850mL,
Meso- and micro-nutrients solution 65mL~67mL, streptomyces microflavus high density bacterium solution 20mL~25mL, bacillus licheniformis are highly dense
Degree bacterium solution 40mL~45mL and Bacillus megatherium high density bacterium solution 40mL~45mL；
Then plus purified water is settled to 1000mL~1050mL, it is 5.5~6.5 to adjust pH value with NaOH,
Finally, with 300r/min~400r/min rotating speeds, 40min~60min is stirred, that is, polynary liquid composite microbe fertilizer is obtained
Material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710100820.1A CN106699416A (en) | 2017-02-23 | 2017-02-23 | Multielement liquid compound microorganism fertilizer and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710100820.1A CN106699416A (en) | 2017-02-23 | 2017-02-23 | Multielement liquid compound microorganism fertilizer and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106699416A true CN106699416A (en) | 2017-05-24 |
Family
ID=58916923
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710100820.1A Pending CN106699416A (en) | 2017-02-23 | 2017-02-23 | Multielement liquid compound microorganism fertilizer and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106699416A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108101646A (en) * | 2017-12-29 | 2018-06-01 | 林立森 | A kind of preparation method of flowers fruits and vegetables biological organic Liquid Fertilizer |
| CN110256148A (en) * | 2019-06-27 | 2019-09-20 | 东莞一翔液体肥料有限公司 | A kind of diseases prevention promoting root growth microbial-bacterial fertilizer and preparation method thereof suitable for water-fertilizer integral system |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101857500A (en) * | 2010-06-21 | 2010-10-13 | 山东宝源生物有限公司 | Microbe organic fertilizer containing chelated microelements and production method thereof |
| CN103772011A (en) * | 2013-12-27 | 2014-05-07 | 天津北洋百川生物技术有限公司 | Special compound biological liquid-state bacterial fertilizer for grain |
| CN104817393A (en) * | 2015-04-24 | 2015-08-05 | 漳州三本肥料工业有限公司 | Method of preparing composite full nutrition type microbial liquid fertilizer |
| CN105237228A (en) * | 2015-11-02 | 2016-01-13 | 山西年马肥业有限公司 | Liquid biological organic fertilizer with high content of organic substances and preparation method |
-
2017
- 2017-02-23 CN CN201710100820.1A patent/CN106699416A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101857500A (en) * | 2010-06-21 | 2010-10-13 | 山东宝源生物有限公司 | Microbe organic fertilizer containing chelated microelements and production method thereof |
| CN103772011A (en) * | 2013-12-27 | 2014-05-07 | 天津北洋百川生物技术有限公司 | Special compound biological liquid-state bacterial fertilizer for grain |
| CN104817393A (en) * | 2015-04-24 | 2015-08-05 | 漳州三本肥料工业有限公司 | Method of preparing composite full nutrition type microbial liquid fertilizer |
| CN105237228A (en) * | 2015-11-02 | 2016-01-13 | 山西年马肥业有限公司 | Liquid biological organic fertilizer with high content of organic substances and preparation method |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108101646A (en) * | 2017-12-29 | 2018-06-01 | 林立森 | A kind of preparation method of flowers fruits and vegetables biological organic Liquid Fertilizer |
| CN110256148A (en) * | 2019-06-27 | 2019-09-20 | 东莞一翔液体肥料有限公司 | A kind of diseases prevention promoting root growth microbial-bacterial fertilizer and preparation method thereof suitable for water-fertilizer integral system |
| CN110256148B (en) * | 2019-06-27 | 2022-04-22 | 东莞一翔液体肥料有限公司 | Disease-preventing root-promoting microbial fertilizer applicable to water-fertilizer integrated system and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100486941C (en) | Whole-nutrition biological flushing and spreading fertilizer and preparation method thereof | |
| CN102229900B (en) | Compound probiotics preparation for biological organic fertilizers and preparation method thereof | |
| CN101659934B (en) | Antagonistic bacteria preventing and removing continuous cropping banana Panama wilt disease and microbial organic fertilizer thereof | |
| CN101857500B (en) | Microbe organic fertilizer containing chelated microelements and production method thereof | |
| CN103011961B (en) | Active organic bacterial manure and preparation method thereof | |
| CN103570434B (en) | A kind of microbial inoculum, liquid microbe fertilizer, its production method and application | |
| CN101671205B (en) | Composite microbial preparation for promoting growth of rape and preparation method thereof | |
| CN102001870A (en) | Inorganic-organic microbial compound fertilizer and preparation method thereof | |
| CN105330486B (en) | A kind of the solanaceous vegetables special nutrient set fertilizer and its method of administration of biological and ecological methods to prevent plant disease, pests, and erosion synergy | |
| CN102382810A (en) | Preparation method of probiotic inoculum for preventing replant disease of greenhouse cucumbers | |
| CN106544302A (en) | A kind of complex micro organism fungicide for soil improvement and preparation method thereof | |
| CN103834565A (en) | Micro-ecological flora of crop root system probiotics and application thereof | |
| CN102210332A (en) | Microbial agent for promoting rootage, preventing continuous cropping and performing ecological restoration on soil for planting bananas and preparation method thereof | |
| CN102021118A (en) | Preparation method of liquid inoculant of aboriginal probiotic bacillus megaterium, bacillus mucilaginosus and azotobacter chroococcum | |
| CN106699474A (en) | Novel bio-organic fertilizer and preparation method thereof | |
| CN102559564B (en) | Method for culturing paenibacillus polymyxa at high density | |
| CN104447054A (en) | Special biological organic fertilizer for tea | |
| CN105505843B (en) | One plant of Photosynthetic bacterium strain, the Liquid Fertilizer containing the bacterial strain and preparation method, application | |
| CN103420710A (en) | Special fertilizer for ornamental flower plants | |
| CN102242075A (en) | Semi-continuous fermentation method for bacillus used for microbial fertilizer | |
| CN106699416A (en) | Multielement liquid compound microorganism fertilizer and preparation method thereof | |
| CN104478498B (en) | It is a kind of for microorganism formulation of middle-and-low-yielding fields restoration of the ecosystem and preparation method thereof | |
| CN102598978A (en) | Method for improving chromium resistance and cadmium resistance of Festuca arundinacea with compost fermentation liquid | |
| CN102598977B (en) | Method for improving chromium resistance and cadmium resistance of ryegrass with compost fermentation liquid | |
| CN102191205B (en) | Bacterial strain B1 for converting insoluble phosphate into soluble phosphate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170524 |