CN101857500B - Microbe organic fertilizer containing chelated microelements and production method thereof - Google Patents
Microbe organic fertilizer containing chelated microelements and production method thereof Download PDFInfo
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- CN101857500B CN101857500B CN2010102044133A CN201010204413A CN101857500B CN 101857500 B CN101857500 B CN 101857500B CN 2010102044133 A CN2010102044133 A CN 2010102044133A CN 201010204413 A CN201010204413 A CN 201010204413A CN 101857500 B CN101857500 B CN 101857500B
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- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 title abstract description 9
- 239000003337 fertilizer Substances 0.000 claims abstract description 42
- 241000193388 Bacillus thuringiensis Species 0.000 claims abstract description 17
- 241000223260 Trichoderma harzianum Species 0.000 claims abstract description 17
- 229940097012 bacillus thuringiensis Drugs 0.000 claims abstract description 17
- 230000003042 antagnostic effect Effects 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 7
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 5
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 58
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 30
- 238000000855 fermentation Methods 0.000 claims description 27
- 230000004151 fermentation Effects 0.000 claims description 27
- 235000013379 molasses Nutrition 0.000 claims description 23
- 244000005700 microbiome Species 0.000 claims description 20
- 239000002361 compost Substances 0.000 claims description 18
- 235000015097 nutrients Nutrition 0.000 claims description 18
- 241000894006 Bacteria Species 0.000 claims description 15
- 239000011368 organic material Substances 0.000 claims description 15
- 239000000618 nitrogen fertilizer Substances 0.000 claims description 11
- 239000002054 inoculum Substances 0.000 claims description 10
- 239000003352 sequestering agent Substances 0.000 claims description 10
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 8
- 230000000845 anti-microbial effect Effects 0.000 claims description 8
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 7
- WZLMXYBCAZZIRQ-UHFFFAOYSA-N [N].[P].[K] Chemical compound [N].[P].[K] WZLMXYBCAZZIRQ-UHFFFAOYSA-N 0.000 claims description 7
- 150000001413 amino acids Chemical class 0.000 claims description 7
- 239000011701 zinc Substances 0.000 claims description 7
- 229910052725 zinc Inorganic materials 0.000 claims description 7
- 235000009392 Vitis Nutrition 0.000 claims description 6
- 241000219095 Vitis Species 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 5
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 claims description 5
- 239000011609 ammonium molybdate Substances 0.000 claims description 5
- 235000018660 ammonium molybdate Nutrition 0.000 claims description 5
- 229940010552 ammonium molybdate Drugs 0.000 claims description 5
- 229910021538 borax Inorganic materials 0.000 claims description 5
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 5
- 239000004327 boric acid Substances 0.000 claims description 5
- 229910052796 boron Inorganic materials 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 5
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 5
- 239000011790 ferrous sulphate Substances 0.000 claims description 5
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 5
- 230000005484 gravity Effects 0.000 claims description 5
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 5
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 5
- 239000004328 sodium tetraborate Substances 0.000 claims description 5
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 5
- 230000006641 stabilisation Effects 0.000 claims description 5
- 238000011105 stabilization Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 5
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 5
- 229960001763 zinc sulfate Drugs 0.000 claims description 5
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 4
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 claims description 4
- 230000008485 antagonism Effects 0.000 claims description 4
- 229910052802 copper Inorganic materials 0.000 claims description 4
- 239000010949 copper Substances 0.000 claims description 4
- 229910052742 iron Inorganic materials 0.000 claims description 4
- 229910052750 molybdenum Inorganic materials 0.000 claims description 4
- 239000011733 molybdenum Substances 0.000 claims description 4
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 3
- 239000002921 fermentation waste Substances 0.000 claims description 3
- 229910052748 manganese Inorganic materials 0.000 claims description 3
- 239000011572 manganese Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 238000009335 monocropping Methods 0.000 abstract description 14
- 235000017060 Arachis glabrata Nutrition 0.000 abstract description 9
- 244000105624 Arachis hypogaea Species 0.000 abstract description 9
- 235000010777 Arachis hypogaea Nutrition 0.000 abstract description 9
- 235000018262 Arachis monticola Nutrition 0.000 abstract description 9
- 235000020232 peanut Nutrition 0.000 abstract description 9
- 201000010099 disease Diseases 0.000 abstract description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 8
- 239000000463 material Substances 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 241001361634 Rhizoctonia Species 0.000 abstract 1
- 230000009920 chelation Effects 0.000 abstract 1
- 239000002689 soil Substances 0.000 description 19
- 239000011573 trace mineral Substances 0.000 description 11
- 235000013619 trace mineral Nutrition 0.000 description 11
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 10
- 239000002699 waste material Substances 0.000 description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 8
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 8
- 235000011130 ammonium sulphate Nutrition 0.000 description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 7
- 229910052799 carbon Inorganic materials 0.000 description 7
- 235000001014 amino acid Nutrition 0.000 description 6
- 239000006052 feed supplement Substances 0.000 description 6
- 239000012530 fluid Substances 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 5
- 229930006000 Sucrose Natural products 0.000 description 5
- 235000019270 ammonium chloride Nutrition 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000005720 sucrose Substances 0.000 description 5
- 238000012366 Fed-batch cultivation Methods 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 125000001477 organic nitrogen group Chemical group 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000009264 composting Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 208000012639 Balance disease Diseases 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010022971 Iron Deficiencies Diseases 0.000 description 1
- 241000784728 Lycaena virgaureae Species 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 241000918585 Pythium aphanidermatum Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000003627 allelochemical Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000003967 crop rotation Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 231100000880 dysequilibrium Toxicity 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000009336 multiple cropping Methods 0.000 description 1
- 235000018343 nutrient deficiency Nutrition 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 244000000034 soilborne pathogen Species 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to a microbe organic fertilizer containing chelated microelements and a production method thereof. The fertilizer contains rich antagonistic microbes, i.e. trichoderma harzianum, bacillus subtilis and bacillus thuringiensis, and the chelated microelements with an extremely high chelation rate, and while preventing and treating continuous cropping obstacles and increasing the crop yield, the fertilizer can supply needed microelements to crops. The sources of the materials for the production of the fertilizer are wide and rich, the cost is low, the technique is simple, the product price is low, the fertilizer is highly specific, the market space is large, and the comprehensive cost performance is high. The yield of peanut can be increased by 69.9 percent, soil-borne diseases, such as root rot and rhizoctonia rot, can be reduced by 80.6 percent, and disease-preventing and yield-increasing effects are remarkable.
Description
Technical field
The present invention relates to intensive agriculture and produce biochemical technology, be specifically related to a kind of microorganism organic fertilizer and production method thereof that contains chelated microelements.This fertilizer not only contains abundant antagonistic microbe, and is rich in the trace element of chelating attitude.
Background technology
Because single, the multiple cropping rate height of modern intensive agricultural planting crop species, chemical fertilizer usage quantity are big, cause soil physico-chemical property to change, the soil micro-ecosystem dysequilibrium, and then bring out continuous cropping obstacles and frequently take place, had a strong impact on the Sustainable development of agricultural output, quality and agricultural.In recent years, not only continuous cropping obstacle constantly takes place in the facility cultivation, and the plot of cultivations such as field crop such as cotton, peanut, fruit tree also descends because of continuous cropping obstacle causes output, quality, and this phenomenon is expansion trend.
At present people often adopt measures control continuous cropping obstacles such as crop rotation, improving soil fertility, soil disinfection, but poor effect, along with people to the going deep into of soil micro-ecosystem environmental research, biological control becomes the focus of present Chinese scholars research.Behind the continuous cropping, because the variation of its soil physico-chemical property and illumination, humiture, gaseous fraction, the growth of some beneficial microorganisms (ammonium bacterium, nitrifier etc.) is suppressed, and some harmful microorganisms obtain breeding rapidly, the Balance of nature of soil microorganisms is destroyed, the obstacle that so not only causes the fertilizer decomposition course, and disease and pest take place many, spread soon, and increase the weight of year by year.Studies show that in a large number and utilize antagonistic microbe can effectively suppress soil-borne disease microbial growth and degraded allelochemical, prevent and kill off continuous cropping obstacles effectively.
In order to improve crop yield, people only pay close attention to the input of macroelement such as N, P, K for a long time, have ignored micro-replenishing in the soil.In fact, there is a large amount of soil all to be in to lack or seriously lack the state of trace element in China.China's soil lacking boron area lacks the zinc area more than 20% more than 40%, and manganese deficiency, iron deficiency, scarce copper face amass and be respectively about 10%, 5%, 1%, and the practical study proof can obviously improve crop yield and quality by trace element fertilizer in enriching.The fertilizer efficiency that studies show that chelated microelements normally inorganic little fertilizer 2-5 doubly, wherein the fertilizer efficiency of chelating attitude zinc fertilizer can reach 10 times of inorganic zinc fertilizer.But the unit cost of chelated trace fertilizer expense generally exceeds 10 times than inorganic little fertilizer, is the key that chelated trace fertilizer has competitive capacity and find out sequestrant cheap and easy to get preferably.
In sum, Recent study utilizes organic waste to become focus as the carrier of antagonistic microbe in the middle of the microbial fertilizer, and seeks the key that the sequestrant inexpensive, that performance is good also becomes trace element fertilizer research.So microbial fertilizer and trace element fertilizer are effectively combined, will become the focus of following fertilizer industry research.
Summary of the invention
The object of the present invention is to provide a kind of microorganism organic fertilizer and production method thereof that contains chelated microelements.
The present invention contains the microorganism organic fertilizer of chelated microelements, contains following components in part by weight:
Nitrogen phosphorus potassium total nutrient 8～12
Organic 20～40
Chelated microelements 2～6; And
Antagonistic microbe 〉=5.0 * 10
8Cfu/g.
Preferred fertilizer of the present invention contains following components in part by weight:
Nitrogen phosphorus potassium total nutrient 10
Organic 30
Chelated microelements 3; And
Antagonistic microbe 5.0 * 10
8-1.0 * 10
10Cfu/g.
The present invention is the target germ with multiple different soil-borne disease pathogenic bacterium, employing face-off culture method filters out the antagonism bacterial classification and makes up from some microorganisms that do not belong to together, plant, and it is trichoderma harzianum (Trichoderma harzianum), subtilis (Bacillus subtilis), bacillus thuringiensis (Bacillus thuringiensis) that the result preferably makes up.
Preferably contain in the described fertilizer: trichoderma harzianum (Trichoderma harzianum) 1.8 * 10
8-3.8 * 10
8Cfu/g, subtilis (Bacillus subtilis) 1.8 * 10
8-3.8 * 10
8Cfu/g, bacillus thuringiensis (Bacillus thuringiensis) 1.4 * 10
8-3.8 * 10
8Cfu/g.
The sequestrant of described chelated microelements is preferably molasses fermented liquid, and more than preferred 35% (w/v) of its aminoacids content, described chelated microelements can be selected from: one or more in boron, molybdenum, manganese, zinc, copper, the iron.Preferred described chelated microelements is by weight: boron 1.5-2.5, molybdenum 0.5-1, manganese 0.6-0.9, zinc 0.5-1.2, copper 1-2, iron 1-2.5.
The present invention also provides the production method of above-mentioned fertilizer, and it may further comprise the steps:
1) preparation antagonism fermented liquid 1. with trichoderma harzianum, subtilis, bacillus thuringiensis activated after, be inoculated in respectively in the liquid PDA substratum, under the 20-40 ℃ of condition, 180-300r/min, shaking table is cultivated 2-3d, treats bacterium liquid OD
600Stop between the value 3.0-4.0 cultivating, make first order seed; 2. press the inoculum size of the 5-20% of liquid nutrient medium volume, the first order seed that makes is inoculated in respectively in the fermentor tank, under the 20-40 ℃ of condition, keep rotating speed 180-300r/min, air flow 1: 0.5-1.5 cultivates 2-3d, makes secondary seed; 3. press the inoculum size of the 5-20% of liquid nutrient medium volume, secondary seed is inoculated in carries out the high density fermentation cultivation in the fermentor tank, obtain high gravity fermentation liquid, put a jar condition: living bacteria count 〉=1.0 * 10
10Individual/ml;
2) with the molasses fermented liquid of sequestrant with amino acid contained and boric acid, borax, copper sulfate, ferrous sulfate, manganous sulfate, zinc sulfate, ammonium molybdate 1: 1: 0.2 in molar ratio: 0.7: 0.8: 0.3: mix at 0.6: 0.5, regulating the pH value with sodium hydroxide is 7.0-8.0, stir, 25 ± 3 ℃ of reaction 1h form the molasses fermented liquid of chelating;
3) with dry weight basis with vinasse, Pericarpium Vitis viniferae, inorganic nitrogenous fertilizer with 40-50: 50-60: 1-3 mixing secondary fermentation, treat to keep when compost temperature rises to 60-70 ℃ 5-7d, compost temperature is kept 5-7d at 50-60 ℃, after reaching 50 ℃, temperature of every 2-3d turning turns over, the 0.5-1% that press fermentation materials weight during each turning adds inorganic nitrogenous fertilizer and presses 0.5-1% (V/W) the interpolation vinasse fermentation waste liquid of fermentation materials, compost temperature stops compost behind the 50-60 ℃ of 3-5d that begins to lower the temperature, obtain antimicrobial organic materials carrier short of money;
4) in organic materials carrier per ton, add the zymocyte liquid 10-50L that step 1) makes, step 2) the molasses fermented liquid 2500～3500kg of chelating that makes, mix.
Wherein, the step 1) high density fermentation is cultivated and can be taked following condition to carry out: substratum is PDA, culture temperature 25-35 ℃, with sodium hydroxide with the pH value stabilization between 6.0-7.0, dissolved oxygen early stage 100-80%, mid-term 80-40%, later stage 60-40%, last 100%, put a jar condition: living bacteria count 〉=1.0 * 10
10Individual/ml.And can adopt the fed batch cultivation mode, used feed supplement carbon source is: glucose, sucrose or molasses; Nitrogenous source is: ammonium sulfate or ammonium chloride.
Wherein the step 3) described inorganic nitrogenous fertilizer that is used to ferment can be ammonium sulfate, ammonium nitrate, ammonium chloride or urea.
A kind of microorganism organic fertilizer that contains chelated microelements of the present invention, be to utilize in commercial fermentation waste thing, the Pericarpium Vitis viniferae in the abundant organism of fermentation short period of time a large amount of carbon sources and inorganic nitrogen changed into to contain rich soluble carbon and the higher organic materials of organonitrogen content, mix with the antagonistic microbe fermented liquid again, add simultaneously with molasses fermented liquid is the trace element of sequestrant, the microorganism organic fertilizer of making, compare with product on the market, it has the following advantages:
1) contains the efficient antagonistic strain (trichoderma harzianum, subtilis, bacillus thuringiensis) of preventing and treating continuous cropping obstacles in this fertilizer, can effectively suppress the growth of the common soil-borne pathogen of continuous cropping obstacle.Organic content is can be more than 30% in the fertilizer, rich soluble carbon, organonitrogen and mineral substance, can offer the suitable growing environment of antagonistic microbe, greatly improved the ability of the field planting of antagonistic microbe in soil and performance function, promote the breeding of soil original inhabitants profitable strain simultaneously, keep the balance of soil microenvironment.
2) contain a large amount of yeast saccharomyces cerevisiaes in the antagonistic microbe organic materials carrier of this fertilizer, need not to add other microorganisms and just can promote the rapid fermentation maturity of compost, general spring and summer 15d, winter, 20-25d just can obtain the high decomposed organic matter fertilizer of N-P-K content.
3) these fertiliser production raw material sources extensively enrich, and cost is low, and technology is simple, and the product price is low, and is with strong points, has huge social, environment and economic benefit.
4) this fertilizer contains a large amount of chelated microelements, and is in stable condition, the utilization ratio height.Can alleviate the crop nutritional deficiency symptom effectively, improve crop quality, the market space is big, comprehensive price ratio height.
A kind of microorganism organic fertilizer that contains chelated microelements of the present invention, it contains the antagonistic microbe of multiple soil-borne diseases such as can preventing and treating damping-off, southern blight, root rot, and the trace element of chelating attitude.Overcome the common disease of succession crop and change sense from poison, effect reaches more than 80%, and trace element supplement keeps soil nutrient balance, guarantees the Sustainable development of agricultural.
Description of drawings
Fig. 1 is the microorganism organic fertilizer Production Flow Chart that the present invention contains chelated microelements.
Embodiment
The microorganism organic fertilizer that the present invention contains chelated microelements is prepared according to flow process shown in Figure 1, specifically comprises the steps:
The original strain of three strain bacterial strain trichoderma harzianums, subtilis, bacillus thuringiensis is being inoculated in respectively under the aseptic condition on the PDA solid medium, is cultivating 2-3d under the 20-40 ℃ of condition, making actication of culture.The activatory bacterial classification is inoculated in respectively under aseptic condition in the liquid PDA substratum, under the 20-40 ℃ of condition, 180-300r/min, shaking table is cultivated 2-3d, treats bacterium liquid OD
600Stop between the value 3.0-4.0 cultivating, make first order seed.Press the inoculum size of the 5-20% of liquid nutrient medium volume, the first order seed that makes is inoculated in (substratum is liquid PDA) in the fermentor tank respectively, under the 20-40 ℃ of condition, keep rotating speed 180-300r/min, air flow 1: 0.5-1.5 cultivates 2-3d, makes secondary seed.Press the inoculum size of the 5-20% of liquid nutrient medium volume, secondary seed is inoculated in carries out high density fermentation in the fermentor tank and cultivate, substratum is PDA (starch, sucrose, molasses and an ammonium sulfate etc.), culture temperature 25-35 ℃, with sodium hydroxide with the pH value stabilization between 6.0-7.0, dissolved oxygen early stage 100-80% (0-10h), mid-term 80-40% (11～30h), (31～39h), last 100% (40～finish) put a jar condition: living bacteria count 〉=1.0 * 10 to later stage 60-40%
10Individual/ml, fermentation 48h obtains high gravity fermentation liquid.High density fermentation is cultivated and is adopted the fed batch cultivation mode, and wherein feed supplement carbon source is glucose, sucrose, molasses; Nitrogenous source is ammonium sulfate, ammonium chloride.
With dry weight basis with vinasse, Pericarpium Vitis viniferae, inorganic nitrogenous fertilizer with 40-50: 50-60: 1-3 mixing secondary fermentation, treat to keep when compost temperature rises to 60-70 ℃ 5-7d, compost temperature is kept 5-7d at 50-60 ℃, every 2-3d turning once, add inorganic nitrogenous fertilizer and the fermented waste fluid of 0.5-1% during each turning, compost temperature stops at once from the 50-60 ℃ of 3-5d that begins to lower the temperature, and obtains antimicrobial organic materials carrier short of money.To add fermented waste fluid be the vinasse waste liquid that is crushed to 1-5cm, the nitrogen of used antimicrobial organic materials carrier short of money is organic nitrogen more than 90%.
With the molasses fermented liquid of sequestrant (available from the Taiwan red group that distinguishes the flavor of) with contained aminoacids complex wherein and boric acid, borax, copper sulfate, ferrous sulfate, manganous sulfate, zinc sulfate, ammonium molybdate 1: 1: 0.2 in molar ratio: 0.7: 0.8: 0.3: mix at 0.6: 0.5, regulating the pH value with sodium hydroxide is 7.0-8.0, stir, 25 ± 3 ℃ of reaction 1h, form the molasses fermented liquid of chelating, wherein trace element chelated rate can reach more than 90%.
Add zymocyte liquid 10-50L in the SOLID ORGANIC material carrier per ton, the molasses fermented liquid 2500～3500kg of chelating mixes.After testing, make and contain antagonism bacterium quantity and reach 5.0 * 10
8Cfu/g-1.0 * 10
10Cfu/g, nitrogen phosphorus potassium total nutrient 8-12%, organic 20-40%, the microorganism organic fertilizer of trace element more than 2%.
Following examples further specify content of the present invention, but should not be construed as limitation of the present invention.Without departing from the spirit and substance of the case in the present invention, modification or replacement to the inventive method, step or condition are done all belong to scope of the present invention.
If do not specialize the conventional means that used technique means is well known to those skilled in the art among the embodiment.
Embodiment 1
The original strain of three strain bacterial strain trichoderma harzianums, subtilis, bacillus thuringiensis is being inoculated in respectively under the aseptic condition on the PDA solid medium, is cultivating 2-3d under 35 ℃ of conditions, making actication of culture.The activatory bacterial classification is inoculated in respectively under aseptic condition in the liquid PDA substratum, under 35 ℃ of conditions, 200r/min, shaking table is cultivated 2-3d, treats bacterium liquid OD
600Value 4.0 stops to cultivate, and makes first order seed.Press 10% inoculum size of liquid nutrient medium volume, the first order seed that makes is inoculated in respectively in the fermentor tank, under 35 ℃ of conditions, keep rotating speed 200r/min, air flow 1: 1 is cultivated 3d, makes secondary seed.Press 10% inoculum size of liquid nutrient medium volume, secondary seed is inoculated in carries out high density fermentation in the fermentor tank and cultivate, substratum is PDA, 30 ℃ of culture temperature, with sodium hydroxide with the pH value stabilization between 6.0-7.0, dissolved oxygen early stage 90%, mid-term 60%, later stage 50%, last 100%, put a jar condition: living bacteria count 〉=1.0 * 10
10Individual/ml, fermentation 48h obtains high gravity fermentation liquid.High density fermentation is cultivated and is adopted the fed batch cultivation mode, and wherein feed supplement carbon source is glucose, sucrose, molasses; Nitrogenous source is ammonium sulfate, ammonium chloride.The early stage of dissolved oxygen, mid-term, later stage feed supplement, feed supplement are 5% (W/V) of fermented liquid.
With dry weight basis with vinasse, Pericarpium Vitis viniferae, inorganic nitrogenous fertilizer (ammonium sulfate) to mix secondary fermentation at 45: 53: 2, treat to keep when compost temperature rises to 65 ℃ 6d, compost temperature is kept 6d at 55 ℃, every 2d turning once, press 0.5% interpolation inorganic nitrogenous fertilizer (ammonium sulfate) and fermented waste fluid 0.5% (V/W) of composting material dry weight during each turning, compost temperature stops behind the 50-60 ℃ of 4d that begins to lower the temperature, and obtains antimicrobial organic materials carrier short of money.To add fermented waste fluid be the vinasse waste liquid that is crushed to 1-5cm, the nitrogen of used antimicrobial organic materials carrier short of money is organic nitrogen more than 90%.
With the molasses fermented liquid of sequestrant (aminoacids content＞35% (w/v)) by amino acid contained and boric acid, borax, copper sulfate, ferrous sulfate, manganous sulfate, zinc sulfate, ammonium molybdate 1: 1: 0.2 in molar ratio: 0.7: 0.8: 0.3: mix at 0.6: 0.5, regulating the pH value with sodium hydroxide is 7.5, stir, 25 ℃ of reaction 1h, form the molasses fermented liquid of chelating, wherein trace element chelated rate can reach more than 90%.
Add zymocyte liquid 30L in the SOLID ORGANIC material carrier per ton, the molasses fermented liquid 3000kg of chelating mixes.After testing, trichoderma harzianum reaches 2.2 * 10
8Cfu/g, subtilis 2.5 * 10
8Cfu/g, bacillus thuringiensis 1.8 * 10
8Cfu/g, nitrogen phosphorus potassium total nutrient 10%, organic 35%, micro-2%.
Embodiment 2
The original strain of three strain bacterial strain trichoderma harzianums, subtilis, bacillus thuringiensis is being inoculated in respectively under the aseptic condition on the PDA solid medium, is cultivating 3d under 25 ℃ of conditions, making actication of culture.The activatory bacterial classification is inoculated in respectively under aseptic condition in the liquid PDA substratum, under 30 ℃ of conditions, 180r/min, shaking table is cultivated 3d, treats bacterium liquid OD
600Value 4.0 stops to cultivate, and makes first order seed.Press 20% inoculum size of liquid nutrient medium volume, the first order seed that makes is inoculated in respectively in the fermentor tank, under 37 ℃ of conditions, keep rotating speed 300r/min, air flow 1: 1.5 is cultivated 3d, makes secondary seed.Press 20% inoculum size of liquid nutrient medium volume, secondary seed is inoculated in carries out high density fermentation in the fermentor tank and cultivate, enriched medium is PDA, 35 ℃ of culture temperature, with sodium hydroxide with the pH value stabilization 6.5, dissolved oxygen early stage 80%, mid-term 60%, later stage 50%, last 100%, put a jar condition: living bacteria count 〉=1.0 * 10
10Individual/ml, fermentation 48h obtains high gravity fermentation liquid.High density fermentation is cultivated and is adopted the fed batch cultivation mode, and wherein feed supplement carbon source is glucose, sucrose, molasses; Nitrogenous source is ammonium sulfate, ammonium chloride.
With dry weight basis with vinasse, Pericarpium Vitis viniferae, inorganic nitrogenous fertilizer to mix secondary fermentation at 40: 60: 1, treat to keep when compost temperature rises to 70 ℃ 5d, compost temperature is kept 7d at 60 ℃, every 2-3d turning once, press 0.1% interpolation inorganic nitrogenous fertilizer and fermented waste fluid of composting material weight during each turning, compost temperature stops compost at once behind the 50-60 ℃ of 3-5d that begins to lower the temperature, obtain antimicrobial organic materials carrier short of money.To add fermented waste fluid be the vinasse waste liquid that is crushed to 1-5cm, the nitrogen of used antimicrobial organic materials carrier short of money is organic nitrogen more than 90%.
With the molasses fermented liquid of sequestrant (available from the Taiwan red group that distinguishes the flavor of) by amino acid contained and boric acid, borax, copper sulfate, ferrous sulfate, manganous sulfate, zinc sulfate, ammonium molybdate 1: 1: 0.2 in molar ratio: 0.7: 0.8: 0.3: mix at 0.6: 0.5, regulating the pH value with sodium hydroxide is 7.0, stir, 28 ℃ of reaction 1h form the molasses fermented liquid of chelating.
Add zymocyte liquid 30L in the SOLID ORGANIC material carrier per ton, the molasses fermented liquid 2500kg of chelating mixes.After testing, trichoderma harzianum reaches 2.0 * 10
8Cfu/g, subtilis 1.8 * 10
8Cfu/g, bacillus thuringiensis 1.5 * 10
8Cfu/g, nitrogen phosphorus potassium total nutrient 10%, organic matter 30%, chelated microelement 2%.
Embodiment 3
The microorganism organic fertilizer that contains chelated microelements that utilizes embodiment 1 described method to make, in the Yantai City, Shandong Province, Yantai research institute carries out in the experimental plot fertilizer efficiency and detects.For examination each 60m of sub-district
2, each is handled and is respectively 5 years peanut soil of continuous cropping, does not have the normal soil of continuous cropping, and 5 years soil of continuous cropping is affixed by general goods fertilizer 8kg, and 5 years soil of continuous cropping is affixed by fertilizer 8kg of the present invention.Each handles three repetitions, totally 12 sub-districts.Peanut varieties is selected Shandong flower peanut for use, uses behind the fertilizer treatment insemination and emergence.Treat ripe back results peanut.Experimental result sees Table 1.
Table 1 contains volume increase, the protection effect of the microorganism organic fertilizer of chelated microelements to peanut
Annotate: the alphabetical different table differential of same row is different significantly in the table, and alphabetical identical table differential is different not remarkable, p＜0.05
From experimental result as can be seen, use the microorganism organic fertilizer that the present invention contains chelated microelements, compare volume increase 69.9% with continuous cropping soil plantation peanut, sickness rate reduces by 80.6%, compares volume increase 12.3% with the processing that applies fertilizer.This shows that the microorganism organic fertilizer that the present invention contains chelated microelements can effectively increase peanut yield, prevents and treats soil-borne disease.Has great market popularization value.
Claims (6)
1. microorganism organic fertilizer that contains chelated microelements is characterized in that this fertilizer contains following components in part by weight:
Nitrogen phosphorus potassium total nutrient 8～12
Organic 20～40
Chelated microelements 2～6; And
Antagonistic microbe 〉=5.0 * 10
8Cfu/g,
Wherein, described antagonistic microbe is composited by trichoderma harzianum (Trichoderma harzianum), subtilis (Bacillus subtilis), these three kinds of bacterium of bacillus thuringiensis (Bacillus thuringiensis); The sequestrant of described chelated microelements is molasses fermented liquid, and described chelated microelements is: boron, molybdenum, manganese, zinc, copper and iron.
2. fertilizer according to claim 1 is characterized in that this fertilizer contains following components in part by weight:
Nitrogen phosphorus potassium total nutrient 10
Organic 30
Chelated microelements 3; And
Antagonistic microbe 5.0 * 10
8-1.0 * 10
10Cfu/g.
3. fertilizer according to claim 1 and 2 is characterized in that, contains in this fertilizer:
Trichoderma harzianum (Trichoderma harzianum) 1.8 * 10
8-3.8 * 10
8Cfu/g
Subtilis (Bacillus subtilis) 1.8 * 10
8-3.8 * 10
8Cfu/g
Bacillus thuringiensis (Bacillus thuringiensis) 1.4 * 10
8-3.8 * 10
8Cfu/g.
4. fertilizer according to claim 1 and 2 is characterized in that, described chelated microelements is by weight: boron 1.5-2.5, molybdenum 0.5-1, manganese 0.6-0.9, zinc 0.5-1.2, copper 1-2, iron 1-2.5.
5. method for preparing the described fertilizer of claim 1 may further comprise the steps:
1) preparation antagonism fermented liquid 1. with trichoderma harzianum, subtilis, bacillus thuringiensis activated after, be inoculated in respectively in the liquid PDA substratum, under the 20-40 ℃ of condition, 180-300r/min, shaking table is cultivated 2-3d, treats bacterium liquid OD
600Stop between the value 3.0-4.0 cultivating, make first order seed; 2. press the inoculum size of the 5-20% of liquid nutrient medium volume, the first order seed that makes is inoculated in respectively in the fermentor tank, under the 20-40 ℃ of condition, keep rotating speed 180-300r/min, air flow 1: 0.5-1.5 cultivates 2-3d, makes secondary seed; 3. press the inoculum size of the 5-20% of liquid nutrient medium volume, secondary seed is inoculated in carries out the high density fermentation cultivation in the fermentor tank, obtain high gravity fermentation liquid, put a jar condition: living bacteria count 〉=1.0 * 10
10Individual/ml;
2) with the molasses fermented liquid of sequestrant with amino acid contained and boric acid, borax, copper sulfate, ferrous sulfate, manganous sulfate, zinc sulfate, ammonium molybdate 1: 1: 0.2 in molar ratio: 0.7: 0.8: 0.3: mix at 0.6: 0.5, regulating the pH value with sodium hydroxide is 7.0-8.0, stir, 25 ± 3 ℃ of reaction 1h form the molasses fermented liquid of chelating;
3) with dry weight basis with vinasse, Pericarpium Vitis viniferae, inorganic nitrogenous fertilizer with 40-50: 50-60: 1-3 mixing secondary fermentation, treat to keep when compost temperature rises to 60-70 ℃ 5-7d, compost temperature is kept 5-7d at 50-60 ℃, every 2-3d turning once, add the inorganic nitrogenous fertilizer and 0.5-1% (V/W) the vinasse fermentation waste liquid of 0.5-1% weight ratio during each turning, compost temperature stops immediately from the 50-60 ℃ of back 3-5d that begins to lower the temperature, and obtains antimicrobial organic materials carrier short of money;
4) in organic materials carrier per ton, add the zymocyte liquid 10-50L that step 1) makes, step 2) the molasses fermented liquid 2500～3500kg of chelating that makes, mix.
6. method according to claim 5, it is characterized in that, wherein step 1) high density fermentation culture condition is: substratum is PDA, culture temperature 25-35 ℃, with sodium hydroxide with the pH value stabilization between 6.0-7.0, dissolved oxygen early stage 100-80%, mid-term, 80-40%, later stage 60-40% were last 100%, put a jar condition: living bacteria count 〉=1.0 * 10
10Individual/ml.
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| CN100509709C (en) * | 2006-12-11 | 2009-07-08 | 陈南岭 | Process of twice fermenting garbage-sludge mixture to produce microbial fertilizer |
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