CN107285953A - Composite zymocyte, biological organic fertilizer and preparation method thereof - Google Patents
Composite zymocyte, biological organic fertilizer and preparation method thereof Download PDFInfo
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- CN107285953A CN107285953A CN201710519933.5A CN201710519933A CN107285953A CN 107285953 A CN107285953 A CN 107285953A CN 201710519933 A CN201710519933 A CN 201710519933A CN 107285953 A CN107285953 A CN 107285953A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention provides a kind of composite zymocyte, biological organic fertilizer and preparation method thereof, it is related to technical field of biological fertilizer, the composite zymocyte is main by bacillus subtilis, bacillus licheniformis, bacillus thuringiensis and colloid bacillus cereus composition, the composite zymocyte passes through mutually coordinated between each bacterial strain and mutual cooperation, the microflora of rapidly and efficiently fermented livestock excrement can be realized by foring one, with quickening organic fertilizer fermentation process, shorten fermentation time and improve the fertility and the advantage of disease resistance of plant of further obtained biological organic fertilizer, the biological organic fertilizer prepared using the composite zymocyte, rich in organic matter, being capable of improved soil, improve the yield and quality of crops, alleviate Traditional compost fermentation pollution environment, fermentation raw material nutrient is largely lost in and the low problem of fermentation efficiency.The organic fertilizer can be widely used in the plantation of organic food.
Description
Technical field
The present invention relates to technical field of biological fertilizer, more particularly, to a kind of composite zymocyte, biological organic fertilizer and its system
Preparation Method.
Background technology
With the fast development of China's poultry industry, considerable economic benefit is being created simultaneously for peasant, it is difficult there is also some
With the environmental problem of solution.A large amount of untreated feces of livestock and poultry can severe contamination underground water, surface water, soil and crops
Deng, and baneful influence is also brought to the living environment of resident near plant, while causing some potentially hazardous body.
The processing mode of fowl and animal excrement is varied, there is a variety of sides such as physical method, chemical method, microbe fermentation method
Formula.Wherein, the old method of tradition of fowl and animal excrement ferment making fertilizer is to allow its natural packing to ferment 5~6 months, and such stink dissipates
Hair, not only polluted environment but also caused nutrient to be largely lost in, while these without the rough organic fertilizer of fermentation process due to its own band
There are pathogenic bacteria, will also tend to turn into the major source of infection for propagating crop disease.Review and handle excrement with microbe fermentation method, not only
Fermentation process can be accelerated, shorten fermentation time, the nutritive value for excrement of avoiding waste；And temperature drying can be produced by fermenting
Excrement, saves energy consumption, with low cost, receives the welcome of vast farmerses friend.
Therefore, organic fertilizer fermentation process can be accelerated by researching and developing one kind, shorten fermentation time, and that builds up fertility is compound
Zymophyte, and then fowl and animal excrement is prepared as biological organic fertilizer using the composite zymocyte, to alleviate Traditional compost fermentation pollution
Environment, fermentation raw material nutrient is largely lost in the fertilizer efficiency not strong and low problem of fermentation efficiency, becomes very necessary and urgent.
In view of this, it is special to propose the present invention.
The content of the invention
The first object of the present invention is to provide a kind of composite zymocyte, and the composite zymocyte is uniformly mixed by a variety of bacterial strains
Close and be made, by mutually coordinated and mutual cooperation between each bacterial strain, rapidly and efficiently fermented livestock excrement can be realized by foring one
Microflora, with fermentation process is accelerated, shorten fermentation time and improve the fertilizer of further obtained biological organic fertilizer
The advantage of power and disease resistance of plant.
The second object of the present invention is to provide a kind of biological organic fertilizer, and described biological organic fertilizer contains abundant organic
Matter and nutriment being capable of improved soils, the yield and quality of raising crops.
The third object of the present invention is to provide a kind of preparation method of biological organic fertilizer, and this method is the mistake of sealing and fermenting
Journey, has the advantages that easy to operate, environment-friendly, fermentation raw material nutrient loss is small and fermentation efficiency is high.
A kind of composite zymocyte that the present invention is provided, the composite zymocyte is main by bacillus subtilis, lichens gemma
Bacillus, bacillus thuringiensis and colloid bacillus cereus composition.
Further, clump count of the above-mentioned bacillus subtilis in composite zymocyte is 5~1,000,000,000 cfu/g；Lichens bud
Clump count of the spore bacillus in composite zymocyte is 10~3,000,000,000 cfu/g；Bacterium of the bacillus thuringiensis in composite zymocyte
It is 2~600,000,000 cfu/g to fall number；Clump count of the colloid bacillus cereus in composite zymocyte is 1~500,000,000 cfu/g.
Further, clump count of the bacillus subtilis in composite zymocyte is 6~800,000,000 cfu/g；Bacillus licheniformis
Clump count in composite zymocyte is 15~2,500,000,000 cfu/g；Clump count of the bacillus thuringiensis in composite zymocyte is 3
~5 hundred million cfu/g；Clump count of the colloid bacillus cereus in composite zymocyte is 2~400,000,000 cfu/g.
Further, clump count of the bacillus subtilis in composite zymocyte is 700,000,000 cfu/g；Bacillus licheniformis
Clump count in composite zymocyte is 2,000,000,000 cfu/g；Clump count of the bacillus thuringiensis in composite zymocyte is 400,000,000
cfu/g；Clump count of the colloid bacillus cereus in composite zymocyte is 300,000,000 cfu/g.
A kind of bio-organic fertilizer that the present invention is provided, the biological organic fertilizer is mainly as follows by what is counted by weight
Raw material is prepared：Above-mentioned 20~80 parts of composite zymocyte, 200-400 parts of fowl and animal excrement, 150-200 parts of carbon source and trace element
5~40 parts.
Further, the biological organic fertilizer, the biological organic fertilizer, mainly the following raw material system by counting by weight
It is standby to obtain：Above-mentioned 40~60 parts of composite zymocyte, 260-340 parts of fowl and animal excrement, 160-180 parts of carbon source and trace element 10~30
Part.
Further, the biological organic fertilizer, including the following raw material counted by weight：Above-mentioned composite zymocyte 50
Part, 300 parts of fowl and animal excrement, 170 parts of carbon source and 20 parts of trace element.
Further, above-mentioned carbon source is rice husk, chaff slag, bean dregs, dregs of beans or one kind in wheat bran or at least two mixing
Thing.
Further, above-mentioned trace element be by etc. zinc sulfate, copper sulphate and the boric acid of parts by weight mix.
A kind of preparation method for biological organic fertilizer that the present invention is provided, comprises the following steps：
Step (a)：Sterilized after fowl and animal excrement and carbon source are sufficiently mixed, compound is made, then adding water to mix
The moisture content of material is adjusted to 55-65%, and fermentation material is made；
Step (b)：Composite zymocyte is inoculated in fermentation material made from step (a) and is well mixed, then in 25-35
Sealing and fermenting 15~30 days, obtain biological organic fertilizer at a temperature of DEG C.
Further, the preparation method of the biological organic fertilizer, comprises the following steps：
Step (a)：Sterilized after fowl and animal excrement and carbon source are sufficiently mixed, compound is made, then adding water to mix
The moisture content of material is adjusted to 60%, and fermentation material is made；
Step (b)：Composite zymocyte is inoculated in fermentation material made from step (a) and is well mixed, then at 30 DEG C
At a temperature of sealing and fermenting 24 days, obtain biological organic fertilizer.
Further, the preparation method also includes step (s) drying-granulating：The biological organic fertilizer that step (b) is obtained
It is dried, and is prepared into graininess finished product.
Compared with prior art, beneficial effects of the present invention are：
A kind of composite zymocyte that the present invention is provided, the composite zymocyte is main by bacillus subtilis, lichens gemma
Bacillus, bacillus thuringiensis and colloid bacillus cereus composition.Wherein, bacillus subtilis can suppress miscellaneous during the fermentation
Bacteria growing, is formed with beneficial fermentative microflora, accelerates fermenting speed；Bacillus licheniformis is not only able to suppress the life of pathogenic microorganism
It is long, be further prepared as after fertilizer can also inducing plant itself disease resistance mechanisms so as to strengthening the effect of the disease-resistant performance of plant；Soviet Union
Cloud gold bacillus is further prepared as after fertilizer can be as microbial source low toxic pesticide；Colloid bacillus cereus has Soluble phosphorus, Potassium release
With fixed nitrogen function, be conducive to increasing the fertilizer efficiency of organic fertilizer of the present invention.Composite zymocyte of the present invention between each bacterial strain by mutually assisting
Reconcile and cooperate, foring a microflora that can realize rapidly and efficiently fermented livestock excrement has quickening organic fertilizer
Fermentation process, shortens fermentation time and improves the fertility and the advantage of disease resistance of plant of further obtained biological organic fertilizer.
The present invention provides a kind of biological organic fertilizer, the biological organic fertilizer it is main by above-mentioned composite zymocyte, fowl and animal excrement,
Carbon source and trace element are constituted, wherein, composite zymocyte, which has, accelerates organic fertilizer fermentation process, shortens fermentation time and raising
The fertility of biological organic fertilizer and the advantage of disease resistance of plant；Fowl and animal excrement, carbon source and trace element can carry for the fermentation of fertilizer
For materials such as abundant protein, amino acid, cellulose, hydrocarbon and trace elements.Therefore, it is prepared into by above-mentioned raw materials
The biological organic fertilizer arrived, being capable of improved soil, the yield and quality of raising crops rich in organic matter.
The present invention provides a kind of preparation method of biological organic fertilizer, and equipment needed for this method is simple, easy to operate, bio-safety
Property high, environmental pollution it is small, with that easy to operate, environment-friendly, fermentation raw material nutrient loss is small and fermentation efficiency is high etc. is excellent
Point, alleviates Traditional compost fermentation pollution environment, and fermentation raw material nutrient is largely lost in and the low problem of fermentation efficiency.
Embodiment
Technical scheme is clearly and completely described below in conjunction with embodiment, it is clear that described reality
It is a part of embodiment of the invention to apply example, rather than whole embodiments.Based on the embodiment in the present invention, the common skill in this area
The every other embodiment that art personnel are obtained under the premise of creative work is not made, belongs to the model that the present invention is protected
Enclose.
According to an aspect of the present invention, a kind of composite zymocyte, the composite zymocyte it is main by bacillus subtilis,
Bacillus licheniformis, bacillus thuringiensis and colloid bacillus cereus composition.
The function of each strain raw material is in composite zymocyte of the present invention：
Bacillus subtilis (Bacillus subtilis) itself can synthesize alpha-amylase, protease, lipase, fiber
The enzymes such as plain enzyme, the subtilin produced during bacillus subtilis thalli growth, polymyxins, nystatin, quarter butt
Bacterium peptide isoreactivity material, these active materials have obvious inhibitory action to all kinds of pathogenic bacteria.In composite zymocyte of the present invention
Bacillus subtilis has no pathogenicity due to it, it is possible to secrete a variety of enzymes and antibiotic, while having good fermentation base
The advantage of plinth, can suppress varied bacteria growing during the fermentation, be formed with beneficial fermentative microflora, accelerate fermenting speed.
Bacillus licheniformis (Bacillus licheniformis) is that a kind of common Gram-positive in soil is thermophilic
Hot bacterium, can produce endogenous spore, heat-resisting strong stress resistance, in soil and the surface generally existing of plant, is common in plant
A kind of endophyte, it is nontoxic to people and animals, it is free from environmental pollution.Bacillus licheniformis fast growth, nutritional need are simple, its
The growth that many kinds of substance suppresses pathogenic microorganism can be secreted during growth metabolism, simultaneously, additionally it is possible to which inducing plant itself is disease-resistant
Mechanism is so as to strengthen the effect of the disease-resistant performance of plant.
Dipel (Bacillus thuringiensis), is a kind of production crystal gemma bar including many mutation
Bacterium, the bacterium can produce two big toxoids, i.e. endotoxin and exotoxin, make insect stop taking food, and last insect is because of hungry and poisoning
It is dead.Therefore the bacterium can be as microbial source low toxic pesticide, for preventing and treating Orthoptera, elytrum in biological organic fertilizer of the present invention
The various pests of mesh, Diptera, Hymenoptera, particularly Lepidoptera.
Colloid bacillus cereus (Bacillus mucitaginosus) is also known as silicate bacteria, and colloid bacillus cereus is soil
A kind of middle special bacterium, it can decompose the potassium-bearing mineral of Silicon Valley hydrochlorate and aluminosilicate composition, with Soluble phosphorus, Potassium release and fixed nitrogen
Function.The bacterium can grow on the nitrogen-free agar of the feldspar containing potassium, mica, apatite, ground phosphate rock and other ores, discharge
Phosphorus, potassium and other nutrients.Due to the metabolism of thalline itself, the result of biochemical reaction produce organic acid, amino acid, polysaccharide,
The material that hormone etc. is conducive to plant absorption and utilized.
Composite zymocyte of the present invention, mainly by bacillus subtilis, bacillus licheniformis, bacillus thuringiensis and colloid
Bacillus is uniformly mixed to prepare, wherein, bacillus subtilis can suppress varied bacteria growing during the fermentation, be formed with increasingly
Yeast-like fungi group, accelerates fermenting speed；Bacillus licheniformis is not only able to suppress the growth of pathogenic microorganism, is further prepared as fertilizer
Afterwards can also inducing plant itself disease resistance mechanisms so as to strengthen plant disease-resistant performance effect；Dipel is further prepared as
Can be as microbial source low toxic pesticide after fertilizer；Colloid bacillus cereus has Soluble phosphorus, Potassium release and fixed nitrogen function, is conducive to increase
The fertilizer efficiency of organic fertilizer of the present invention.Composite zymocyte of the present invention forms one by mutually coordinated between each bacterial strain and mutual cooperation
The individual microflora that can realize rapidly and efficiently fermented livestock excrement, which has, accelerates organic fertilizer fermentation process, shortens fermentation time
And improve the fertility and the advantage of disease resistance of plant of further obtained biological organic fertilizer.
In the preferred embodiment of the present invention, clump count of the above-mentioned bacillus subtilis in composite zymocyte is
5~1,000,000,000 cfu/g；Clump count of the bacillus licheniformis in composite zymocyte is 10~3,000,000,000 cfu/g；Bacillus thuringiensis
Clump count in composite zymocyte is 2~600,000,000 cfu/g；Clump count of the colloid bacillus cereus in composite zymocyte is 1~5
Hundred million cfu/g.
In the present invention, above-mentioned bacillus subtilis typical but non-limiting clump count in composite zymocyte is：500000000
Cfu/g, 600,000,000 cfu/g, 700,000,000 cfu/g, 800,000,000 cfu/g, 900,000,000 cfu/g or 1,000,000,000 cfu/g.
In the present invention, above-mentioned bacillus licheniformis typical but non-limiting clump count in composite zymocyte is：1000000000
Cfu/g, 1,300,000,000 cfu/g, 1,600,000,000 cfu/g, 2,000,000,000 cfu/g, 2,300,000,000 cfu/g, 2,600,000,000 cfu/g or 3,000,000,000 cfu/g.
In the present invention, above-mentioned bacillus thuringiensis typical but non-limiting clump count in composite zymocyte is：200000000
Cfu/g, 300,000,000 cfu/g, 400,000,000 cfu/g, 500,000,000 cfu/g or 600,000,000 cfu/g.
In the present invention, above-mentioned colloid bacillus cereus typical but non-limiting clump count in composite zymocyte is：100000000
Cfu/g, 200,000,000 cfu/g, 300,000,000 cfu/g, 400,000,000 cfu/g or 500,000,000 cfu/g.
In above-mentioned preferred embodiment, clump count of the above-mentioned bacillus subtilis in composite zymocyte is 6~800,000,000
cfu/g；Clump count of the bacillus licheniformis in composite zymocyte is 15~2,500,000,000 cfu/g；Bacillus thuringiensis is compound
Clump count in zymophyte is 3~500,000,000 cfu/g；Clump count of the colloid bacillus cereus in composite zymocyte is 2~400,000,000 cfu/
g。
It is preferred that, clump count of the above-mentioned bacillus subtilis in composite zymocyte is 700,000,000 cfu/g；Bacillus licheniformis
Clump count in composite zymocyte is 2,000,000,000 cfu/g；Clump count of the bacillus thuringiensis in composite zymocyte is 400,000,000
cfu/g；Clump count of the colloid bacillus cereus in composite zymocyte is 300,000,000 cfu/g.
According to an aspect of the present invention, a kind of bio-organic fertilizer, mainly the following raw material system by counting by weight
It is standby to obtain：Above-mentioned 20~80 parts of composite zymocyte, 200-400 parts of fowl and animal excrement, 150-200 parts of carbon source and trace element 5~40
Part.
The function of each raw material is in biological organic fertilizer of the present invention：
Composite zymocyte is uniformly mixed to prepare by a variety of bacterial strains, by mutually coordinated and mutual cooperation between each bacterial strain, is formed
One can realize the microflora of rapidly and efficiently fermented livestock excrement, with fermentation process is accelerated, shorten fermentation time
And improve the fertility and the advantage of disease resistance of plant of further obtained biological organic fertilizer.
In the present invention, count by weight, composite zymocyte it is typical but non-limiting be：20 parts, 30 parts, 40 parts,
50 parts, 60 parts, 70 parts or 80 parts.
The nutrient content of fowl and animal excrement enriches very much, and existing substantial amounts of organic matter has the higher npk nutrient of content again,
And these nutritional ingredients are very easy to be decomposed by the microorganisms, and fowl and animal excrement is prepared as after organic fertilizer, soil can be effectively increased
The fertility of earth, the nutrient of abundance is provided for crops.
In the present invention, count by weight, fowl and animal excrement it is typical but non-limiting be：200 parts, 220 parts, 240 parts,
260 parts, 280 parts, 300 parts, 320 parts, 340 parts, 360 parts, 380 parts or 400 parts.
Carbon source is rich in element and abundant amino acid, cellulose, hydrocarbon and micro members such as nitrogen, phosphorus, potassium, calcium, magnesium
The material of element, can provide abundant nutrition for the fermentation of organic fertilizer.The content of carbon source has weight to the growth and breeding of microorganism
The influence wanted.When carbon content is too high, sources of nitrogen necessary to causing microbial growth cannot get enough supplies, and this will
The speed of microbial growth is reduced, with season organic matter decomposition rate reduction, fermentation time is long, the source loss of organic matter
Greatly, cause humification coefficient low；When carbon content is too low, though at this moment nitrogen source obtains enough supplies, development of microorganisms is bred
Required energy source is restricted, and temperature in fermentation process is gone up slowly, excessive nitrogen is sustained out in the way of ammonia,
Organic nitrogen loss is big, while producing niff.
In the present invention, count by weight, carbon source is typical but non-limiting content is 150 parts, 160 parts, 170 parts, 180
Part, 190 parts or 200 parts.
Trace element is added in biological organic fertilizer of the present invention, and the nutrition of abundance can be provided when fertilizer ferments, and then
Improve the yield and quality of agricultural product.
In the present invention, count by weight, trace element is typical but non-limiting content is 5 parts, 10 parts, 15 parts, 20 parts,
30 parts, 35 parts or 40 parts.
Biological organic fertilizer of the present invention is mainly made up of above-mentioned composite zymocyte, fowl and animal excrement, carbon source and trace element, its
In, composite zymocyte, which has, accelerates organic fertilizer fermentation process, shortens fermentation time and improves fertility and the plant of biological organic fertilizer
The advantage of thing disease resistance；Fowl and animal excrement, carbon source and trace element can provide abundant protein, amino for the fermentation of fertilizer
The materials such as acid, cellulose, hydrocarbon and trace element.Therefore, the biological organic fertilizer prepared by above-mentioned raw materials, is rich in
Organic matter, being capable of improved soil, the yield and quality of raising crops.
In the preferred embodiment of the present invention, described biological organic fertilizer, mainly by count by weight as
Lower raw material is prepared：Above-mentioned 40~60 parts of composite zymocyte, 260-340 parts of fowl and animal excrement, 160-180 parts of carbon source and micro member
10~30 parts of element.
In above-mentioned preferred embodiment, the biological organic fertilizer, including the following raw material counted by weight：It is above-mentioned multiple
Close 20 parts of 50 parts of zymophyte, 300 parts of fowl and animal excrement, 170 parts of carbon source and trace element.
In the present invention, by the further adjustment and optimization to each component raw material dosage ratio, so as to further optimize
The fertility of biological organic fertilizer of the present invention.
In the preferred embodiment of the present invention, carbon source is one kind in rice husk, chaff slag, bean dregs, dregs of beans or wheat bran
Or at least two mixture.
In the preferred embodiment of the present invention, trace element be by etc. parts by weight zinc sulfate, copper sulphate and boron
Acid is mixed.
It is above-mentioned preferred embodiment in, above-mentioned zinc sulfate, copper sulphate or boric acid etc. be rich in various trace elements,
It is added in biological organic fertilizer of the present invention, the nutrition of abundance can be provided when fertilizer ferments, be the quality of final products
Lay a solid foundation.
According to an aspect of the present invention, a kind of preparation method of biological organic fertilizer, comprises the following steps：
Step (a)：Sterilized after fowl and animal excrement and carbon source are sufficiently mixed, compound is made, then adding water to mix
The moisture content of material is adjusted to 55-65%, and fermentation material is made；
Step (b)：Composite zymocyte is inoculated in fermentation material made from step (a) and is well mixed, then in 25-35
Sealing and fermenting 15~30 days, obtain biological organic fertilizer at a temperature of DEG C.
The preparation method of biological organic fertilizer of the present invention, this method is sealing and fermenting system after each fermentation raw material is mixed
, with required equipment is simple, easy to operate, biological safety is high, environmental pollution is small, with easy to operate, environment-friendly, hair
Ferment raw material nutrient loss is small and the advantages of high fermentation efficiency, alleviates Traditional compost fermentation pollution environment, fermentation raw material nutrient
It is a large amount of to be lost in and the low problem of fermentation efficiency.
In the preferred embodiment of the present invention, the preparation method of the biological organic fertilizer, comprises the following steps：
Step (a)：Sterilized after fowl and animal excrement and carbon source are sufficiently mixed, compound is made, then adding water to mix
The moisture content of material is adjusted to 60%, and fermentation material is made；
Step (b)：Composite zymocyte is inoculated in fermentation material made from step (a) and is well mixed, then at 30 DEG C
At a temperature of sealing and fermenting 24 days, obtain biological organic fertilizer.
In the preferred embodiment of the present invention, the preparation method of the biological organic fertilizer, in addition to step (s) are dry
Dry granulation：The biological organic fertilizer that step (b) is obtained is dried, and is prepared into graininess finished product.
It is preferred that, tunning, which is dried to moisture content, is less than 20%, and is a granulated into the graininess finished product of 60-100 mesh.
Graininess finished product after the present invention is further dried is more beneficial for packaging and the sale in later stage, meanwhile, biological organic fertilizer is carried out
The loss of nutrient in organic fertilizer can also be avoided after drying.
It is described further below in conjunction with 1~12 pair of technical scheme of embodiment 1~10 and comparative example.
Embodiment 1
A kind of composite zymocyte, mainly by bacillus subtilis, bacillus licheniformis, bacillus thuringiensis and colloid bud
Spore bacillus is uniformly mixed to prepare, wherein,
Clump count of the bacillus subtilis in composite zymocyte is 500,000,000 cfu/g；
Clump count of the bacillus licheniformis in composite zymocyte is 1,000,000,000 cfu/g；
Clump count of the bacillus thuringiensis in composite zymocyte is 200,000,000 cfu/g；
Clump count of the colloid bacillus cereus in composite zymocyte is 100,000,000 cfu/g.
Embodiment 2
A kind of composite zymocyte, mainly by bacillus subtilis, bacillus licheniformis, bacillus thuringiensis and colloid bud
Spore bacillus is uniformly mixed to prepare, wherein,
Clump count of the bacillus subtilis in composite zymocyte is 1,000,000,000 cfu/g；
Clump count of the bacillus licheniformis in composite zymocyte is 3,000,000,000 cfu/g；
Clump count of the bacillus thuringiensis in composite zymocyte is 600,000,000 cfu/g；
Clump count of the colloid bacillus cereus in composite zymocyte is 500,000,000 cfu/g.
Embodiment 3
A kind of composite zymocyte, mainly by bacillus subtilis, bacillus licheniformis, bacillus thuringiensis and colloid bud
Spore bacillus is uniformly mixed to prepare, wherein,
Clump count of the bacillus subtilis in composite zymocyte is 600,000,000 cfu/g；
Clump count of the bacillus licheniformis in composite zymocyte is 1,500,000,000 cfu/g；
Clump count of the bacillus thuringiensis in composite zymocyte is 300,000,000 cfu/g；
Clump count of the colloid bacillus cereus in composite zymocyte is 200,000,000 cfu/g.
Embodiment 4
A kind of composite zymocyte, mainly by bacillus subtilis, bacillus licheniformis, bacillus thuringiensis and colloid bud
Spore bacillus is uniformly mixed to prepare, wherein,
Clump count of the bacillus subtilis in composite zymocyte is 800,000,000 cfu/g；
Clump count of the bacillus licheniformis in composite zymocyte is 2,500,000,000 cfu/g；
Clump count of the bacillus thuringiensis in composite zymocyte is 500,000,000 cfu/g；
Clump count of the colloid bacillus cereus in composite zymocyte is 400,000,000 cfu/g.
Embodiment 5
A kind of composite zymocyte, mainly by bacillus subtilis, bacillus licheniformis, bacillus thuringiensis and colloid bud
Spore bacillus is uniformly mixed to prepare, wherein,
Clump count of the bacillus subtilis in composite zymocyte is 700,000,000 cfu/g；
Clump count of the bacillus licheniformis in composite zymocyte is 2,000,000,000 cfu/g；
Clump count of the bacillus thuringiensis in composite zymocyte is 400,000,000 cfu/g；
Clump count of the colloid bacillus cereus in composite zymocyte is 300,000,000 cfu/g.
Embodiment 6
A kind of biological organic fertilizer, including the following raw material counted by weight：20 parts of the composite zymocyte of embodiment 1, fowl
5 parts of just 200 parts of animal manure, 150 parts of carbon source and trace element；
The preparation method of above-mentioned biological organic fertilizer, comprises the following steps：
Step (a)：Sterilized after described fowl and animal excrement and carbon source are sufficiently mixed, compound is made, then adds water
The moisture content of compound is adjusted to 55%, fermentation material is made；
Step (b)：Above-mentioned composite zymocyte is inoculated in fermentation material made from step (a) and is well mixed, is then existed
Sealing and fermenting 15 days, obtain biological organic fertilizer at a temperature of 25 DEG C.
Embodiment 7
A kind of biological organic fertilizer, including the following raw material counted by weight：80 parts of the composite zymocyte of embodiment 2, fowl
40 parts of just 400 parts of animal manure, 200 parts of carbon source and trace element；
The preparation method of above-mentioned biological organic fertilizer, comprises the following steps：
Step (a)：Sterilized after described fowl and animal excrement and carbon source are sufficiently mixed, compound is made, then adds water
The moisture content of compound is adjusted to 65%, fermentation material is made；
Step (b)：Above-mentioned composite zymocyte is inoculated in fermentation material made from step (a) and is well mixed, is then existed
Sealing and fermenting 30 days, obtain biological organic fertilizer at a temperature of 35 DEG C.
Embodiment 8
A kind of biological organic fertilizer, including the following raw material counted by weight：40 parts of the composite zymocyte of embodiment 3, fowl
10 parts of just 260 parts of animal manure, 160 parts of carbon source and trace element；
The preparation method of above-mentioned biological organic fertilizer, comprises the following steps：
Step (a)：Sterilized after described fowl and animal excrement and carbon source are sufficiently mixed, compound is made, then adds water
The moisture content of compound is adjusted to 58%, fermentation material is made；
Step (b)：Above-mentioned composite zymocyte is inoculated in fermentation material made from step (a) and is well mixed, is then existed
Sealing and fermenting 18 days, obtain biological organic fertilizer at a temperature of 28 DEG C.
Embodiment 9
A kind of biological organic fertilizer, including the following raw material counted by weight：60 parts of the composite zymocyte of embodiment 4, fowl
30 parts of just 340 parts of animal manure, 180 parts of carbon source and trace element；
The preparation method of above-mentioned biological organic fertilizer, comprises the following steps：
Step (a)：Sterilized after described fowl and animal excrement and carbon source are sufficiently mixed, compound is made, then adds water
The moisture content of compound is adjusted to 62%, fermentation material is made；
Step (b)：Above-mentioned composite zymocyte is inoculated in fermentation material made from step (a) and is well mixed, is then existed
Sealing and fermenting 28 days, obtain biological organic fertilizer at a temperature of 32 DEG C.
Embodiment 10
A kind of biological organic fertilizer, including the following raw material counted by weight：50 parts of the composite zymocyte of embodiment 5, fowl
20 parts of just 300 parts of animal manure, 170 parts of carbon source and trace element；
The preparation method of above-mentioned biological organic fertilizer, comprises the following steps：
Step (a)：Sterilized after described fowl and animal excrement and carbon source are sufficiently mixed, compound is made, then adds water
The moisture content of compound is adjusted to 60%, fermentation material is made；
Step (b)：Above-mentioned composite zymocyte is inoculated in fermentation material made from step (a) and is well mixed, is then existed
Sealing and fermenting 24 days, obtain biological organic fertilizer at a temperature of 30 DEG C.
Comparative example 1
A kind of composite zymocyte, is mainly uniformly mixed by bacillus licheniformis, bacillus thuringiensis and colloid bacillus cereus
Close and be made, wherein,
Clump count of the bacillus licheniformis in composite zymocyte is 2,000,000,000 cfu/g；
Clump count of the bacillus thuringiensis in composite zymocyte is 400,000,000 cfu/g；
Clump count of the colloid bacillus cereus in composite zymocyte is 300,000,000 cfu/g.
Comparative example 2
A kind of composite zymocyte, is mainly uniformly mixed by bacillus subtilis, bacillus thuringiensis and colloid bacillus cereus
Close and be made, wherein,
Clump count of the bacillus subtilis in composite zymocyte is 700,000,000 cfu/g；
Clump count of the bacillus thuringiensis in composite zymocyte is 400,000,000 cfu/g；
Clump count of the colloid bacillus cereus in composite zymocyte is 300,000,000 cfu/g.
Comparative example 3
A kind of composite zymocyte, mainly by bacillus subtilis, bacillus licheniformis, bacillus thuringiensis and colloid bud
Spore bacillus is uniformly mixed to prepare, wherein,
Clump count of the bacillus subtilis in composite zymocyte is 400,000,000 cfu/g；
Clump count of the bacillus licheniformis in composite zymocyte is 800,000,000 cfu/g；
Clump count of the bacillus thuringiensis in composite zymocyte is 400,000,000 cfu/g；
Clump count of the colloid bacillus cereus in composite zymocyte is 300,000,000 cfu/g.
Comparative example 4
A kind of composite zymocyte, mainly by bacillus subtilis, bacillus licheniformis, bacillus thuringiensis and colloid bud
Spore bacillus is uniformly mixed to prepare, wherein,
Clump count of the bacillus subtilis in composite zymocyte is 700,000,000 cfu/g；
Clump count of the bacillus licheniformis in composite zymocyte is 700,000,000 cfu/g；
Clump count of the bacillus thuringiensis in composite zymocyte is 100,000,000 cfu/g；
Clump count of the colloid bacillus cereus in composite zymocyte is 100,000,000 cfu/g.
Comparative example 5
A kind of biological organic fertilizer, including the following raw material counted by weight：50 parts of the composite zymocyte of comparative example 1, fowl
20 parts of just 300 parts of animal manure, 170 parts of carbon source and trace element；
The preparation method of above-mentioned biological organic fertilizer, comprises the following steps：
Step (a)：Sterilized after described fowl and animal excrement and carbon source are sufficiently mixed, compound is made, then adds water
The moisture content of compound is adjusted to 60%, fermentation material is made；
Step (b)：Above-mentioned composite zymocyte is inoculated in fermentation material made from step (a) and is well mixed, is then existed
Sealing and fermenting 24 days, obtain biological organic fertilizer at a temperature of 30 DEG C.
Comparative example 6
A kind of biological organic fertilizer, including the following raw material counted by weight：50 parts of the composite zymocyte of comparative example 2, fowl
20 parts of just 300 parts of animal manure, 170 parts of carbon source and trace element；
The preparation method of above-mentioned biological organic fertilizer, comprises the following steps：
Step (a)：Sterilized after described fowl and animal excrement and carbon source are sufficiently mixed, compound is made, then adds water
The moisture content of compound is adjusted to 60%, fermentation material is made；
Step (b)：Above-mentioned composite zymocyte is inoculated in fermentation material made from step (a) and is well mixed, is then existed
Sealing and fermenting 24 days, obtain biological organic fertilizer at a temperature of 30 DEG C.
Comparative example 7
A kind of biological organic fertilizer, including the following raw material counted by weight：50 parts of the composite zymocyte of comparative example 3, fowl
20 parts of just 300 parts of animal manure, 170 parts of carbon source and trace element；
The preparation method of above-mentioned biological organic fertilizer, comprises the following steps：
Step (a)：Sterilized after described fowl and animal excrement and carbon source are sufficiently mixed, compound is made, then adds water
The moisture content of compound is adjusted to 60%, fermentation material is made；
Step (b)：Above-mentioned composite zymocyte is inoculated in fermentation material made from step (a) and is well mixed, is then existed
Sealing and fermenting 24 days, obtain biological organic fertilizer at a temperature of 30 DEG C.
Comparative example 8
A kind of biological organic fertilizer, including the following raw material counted by weight：50 parts of the composite zymocyte of comparative example 4, fowl
20 parts of just 300 parts of animal manure, 170 parts of carbon source and trace element；
The preparation method of above-mentioned biological organic fertilizer, comprises the following steps：
Step (a)：Sterilized after described fowl and animal excrement and carbon source are sufficiently mixed, compound is made, then adds water
The moisture content of compound is adjusted to 60%, fermentation material is made；
Step (b)：Above-mentioned composite zymocyte is inoculated in fermentation material made from step (a) and is well mixed, is then existed
Sealing and fermenting 24 days, obtain biological organic fertilizer at a temperature of 30 DEG C.
Effect example 1
To show biological organic fertilizer of the present invention, rich in organic matter, nutrient is complete, nutrient conversion efficiency and organic matter transformation
The characteristics of rate is high, biological organic fertilizer prepared by existing special Example 6~10 and comparative example 5~8 is supported to the total of organic fermentation fertilizer
Sub-prime amount percentage fractional, quality of organic matter percentage fractional, moisture content percentage are observed and detected respectively, knot
It is really as shown in the table：
As seen from the above table, the embodiment of the present invention 6~10 in the composite zymocyte of comparative example 5 with not containing bacillus subtilis
Biological organic fertilizer is contrasted, and the index such as total nutrient and the content of organic matter of comparative example 5 is significantly less than the embodiment of the present invention 6~10
Content；Biological organic fertilizer contrast with not containing bacillus licheniformis in the composite zymocyte of comparative example 6, the total nutrient of comparative example 6
It is significantly less than the content of the embodiment of the present invention 6~10 with the index such as the content of organic matter.Illustrate comparative example 5 and right by the above results
The fertilizer fermentation of ratio 6 is simultaneously insufficient, bacillus subtilis is also illustrated in composite zymocyte of the present invention from another point of view and withered
Careless bacillus can be very good to accelerate the speed of fertilizer fermentation, make fermentation more abundant rapid.
The embodiment of the present invention 6~10 is with the clump count of comparative example 7 and the bacterial strain of comparative example 8 in composite zymocyte not in this hair
Composite zymocyte in bright scope is contrasted, and the index such as total nutrient and the content of organic matter of comparative example 7 and comparative example 8 is obvious
Less than the content of the embodiment of the present invention 6~10.Thus it can be explained, be mutually coordinated and phase between each bacterial strain of composite zymocyte of the present invention
One mutually coordinated can realize the microflora of rapidly and efficiently fermented livestock excrement, be due to the mutual cooperation between each bacterial strain
Composite zymocyte of the present invention, which has, caused by relation accelerates fermentation process, shortens the function of fermentation time.
Comparative example 9
A kind of composite zymocyte, is mainly uniformly mixed by bacillus subtilis, bacillus licheniformis and colloid bacillus cereus
It is made, wherein,
Clump count of the bacillus subtilis in composite zymocyte is 700,000,000 cfu/g；
Clump count of the bacillus licheniformis in composite zymocyte is 2,000,000,000 cfu/g；
Clump count of the colloid bacillus cereus in composite zymocyte is 300,000,000 cfu/g.
Comparative example 10
A kind of composite zymocyte, is mainly uniformly mixed by bacillus subtilis, bacillus licheniformis and bacillus thuringiensis
Close and be made, wherein,
Clump count of the bacillus subtilis in composite zymocyte is 700,000,000 cfu/g；
Clump count of the bacillus licheniformis in composite zymocyte is 2,000,000,000 cfu/g；
Clump count of the bacillus thuringiensis in composite zymocyte is 400,000,000 cfu/g.
Comparative example 11
A kind of biological organic fertilizer, including the following raw material counted by weight：50 parts of the composite zymocyte of comparative example 5, fowl
20 parts of just 300 parts of animal manure, 170 parts of carbon source and trace element；
The preparation method of above-mentioned biological organic fertilizer, comprises the following steps：
Step (a)：Sterilized after described fowl and animal excrement and carbon source are sufficiently mixed, compound is made, then adds water
The moisture content of compound is adjusted to 60%, fermentation material is made；
Step (b)：Above-mentioned composite zymocyte is inoculated in fermentation material made from step (a) and is well mixed, is then existed
Sealing and fermenting 24 days, obtain biological organic fertilizer at a temperature of 30 DEG C.
Comparative example 12
A kind of biological organic fertilizer, including the following raw material counted by weight：50 parts of the composite zymocyte of comparative example 6, fowl
20 parts of just 300 parts of animal manure, 170 parts of carbon source and trace element；
The preparation method of above-mentioned biological organic fertilizer, comprises the following steps：
Step (a)：Sterilized after described fowl and animal excrement and carbon source are sufficiently mixed, compound is made, then adds water
The moisture content of compound is adjusted to 60%, fermentation material is made；
Step (b)：Above-mentioned composite zymocyte is inoculated in fermentation material made from step (a) and is well mixed, is then existed
Sealing and fermenting 24 days, obtain biological organic fertilizer at a temperature of 30 DEG C.
Effect example 2
9 pieces of soya bean plantation examinations are chosen using the soya bean field of Tieli City of Heilongjiang Province Suihua Area as testing site, in testing site
Field, every piece of 5 mu of experimental plot are tested, test group 1~5 applies the biological organic fertilizer that embodiment 6~10 is provided, and control group 1 applies contrast
Biological organic fertilizer prepared by example 11, control group 2 applies biological organic fertilizer prepared by comparative example 12, and control group 3 applies commercially available Hebei
The auspicious fertile board organic fertilizer of Rui Wofei industry Co., Ltd production, control group 4 does not apply any fertilizer, is used as blank control group.
The last week is sowed in soya bean to be applied fertilizer, every group of dose is 400kg/ mus.Nothing is topdressed and sprayed during growth of soybean
Agricultural chemicals is spilt, the field management during experiment is managed collectively according to local soya bean management mode.Test index is the per mu yield of soya bean
The situation of soil when amount, the 100-grain weight of soya bean and harvest.Result of the test is as shown in the table：
As can be seen from the above table, the test group 1-5 of the biological organic fertilizer provided using the present invention soya bean per mu yield is reached
More than 430kg/ mus, the 100-grain weight of soya bean beats more than 30.5g, and leaf during harvest on soya bean rhizome is still cyan, illustrates soil
Earth still also has fertilizer efficiency, and it is not hardened to detect soil.
Control group 1 applies the biological organic fertilizer that bacillus thuringiensis is not contained in the composite zymocyte of comparative example 11, soya bean
Per mu yield is 402kg/ mus, and the 100-grain weight of soya bean is 26.7g, is below test group 1-5；Control group 2 is applied comparative example 12 and is combined
The biological organic fertilizer of colloid bacillus cereus is not contained in zymophyte, soya bean per mu yield is 402kg/ mus, and the 100-grain weight of soya bean is
26.4g, is below test group 1-5, and soya bean rhizome has begun to wither during harvest, illustrates soil without fertilizer efficiency, but examine
Measuring soil does not have hardened, and the high fertilizer efficiency for illustrating the biological organic fertilizer of the present invention is determined by its raw material components and proportioning, is needed
Want each raw material to combine, it is mutually coordinated, produce actively synergy, allow the biological organic fertilizer of production that there is high fertilizer efficiency.
Control group 3 uses the auspicious fertile board organic fertilizer that commercially available Hebei Rui Wofei industry Co., Ltd produces, and soya bean per mu yield is
380kg/ mus, the 100-grain weight of soya bean is 25.7g, and soya bean rhizome has begun to wither during harvest, illustrates soil without fertilizer efficiency,
But it is not hardened to detect soil.
Control group 4 is unmanured blank control group, and soya bean per mu yield and 100-grain weight be minimum, and soya bean rhizome has been during harvest
Through withered, illustrate soil already without fertilizer efficiency, and it is hardened to detect soil.
In summary, composite zymocyte of the present invention forms one by mutually coordinated between each bacterial strain and mutual cooperation
Can realize rapidly and efficiently fermented livestock excrement microflora have accelerate organic fertilizer fermentation process, shorten fermentation time with
And improve the fertility and the advantage of disease resistance of plant of further obtained biological organic fertilizer.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；To the greatest extent
The present invention is described in detail with reference to foregoing embodiments for pipe, it will be understood by those within the art that：Its according to
The technical scheme described in foregoing embodiments can so be modified, or which part or all technical characteristic are entered
Row equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme.
Claims (10)
1. a kind of composite zymocyte, it is characterised in that the composite zymocyte is main by bacillus subtilis, lichens gemma bar
Bacterium, bacillus thuringiensis and colloid bacillus cereus composition.
2. composite zymocyte according to claim 1, it is characterised in that the bacillus subtilis is in composite zymocyte
Clump count be 5~1,000,000,000 cfu/g；Clump count of the bacillus licheniformis in composite zymocyte is 10~3,000,000,000 cfu/g；
Clump count of the bacillus thuringiensis in composite zymocyte is 2~600,000,000 cfu/g；The colloid bacillus cereus is compound
Clump count in zymophyte is 1~500,000,000 cfu/g.
3. composite zymocyte according to claim 2, it is characterised in that the bacillus subtilis is in composite zymocyte
Clump count be 6~800,000,000 cfu/g；Clump count of the bacillus licheniformis in composite zymocyte is 15~2,500,000,000 cfu/g；
Clump count of the bacillus thuringiensis in composite zymocyte is 3~500,000,000 cfu/g；The colloid bacillus cereus is compound
Clump count in zymophyte is 2~400,000,000 cfu/g；
It is preferred that, clump count of the bacillus subtilis in composite zymocyte is 700,000,000 cfu/g；The bacillus licheniformis
Clump count in composite zymocyte is 2,000,000,000 cfu/g；Clump count of the bacillus thuringiensis in composite zymocyte is 4
Hundred million cfu/g；Clump count of the colloid bacillus cereus in composite zymocyte is 300,000,000 cfu/g.
4. a kind of biological organic fertilizer, it is characterised in that the biological organic fertilizer, the main the following raw material system by counting by weight
It is standby to obtain：200-400 parts of 20~80 parts of composite zymocyte, fowl and animal excrement, carbon source 150- described in any one of claims 1 to 3
200 parts and 5~40 parts of trace element.
5. biological organic fertilizer according to claim 4, it is characterised in that the biological organic fertilizer, mainly by by weight
The following raw material of number meter is prepared：40~60 parts of composite zymocyte, fowl and animal excrement described in any one of claims 1 to 3
10~30 parts of 260-340 parts, 160-180 parts of carbon source and trace element；
It is preferred that, the biological organic fertilizer, including the following raw material counted by weight：Described in any one of claims 1 to 3
20 parts of 50 parts of composite zymocyte, 300 parts of fowl and animal excrement, 170 parts of carbon source and trace element.
6. the biological organic fertilizer according to claim 4 or 5, it is characterised in that the carbon source is rice husk, chaff slag, bean dregs, beans
One kind or at least two mixture in the dregs of rice or wheat bran.
7. the biological organic fertilizer according to claim 4 or 5, it is characterised in that trace element be by etc. parts by weight sulfuric acid
Zinc, copper sulphate and boric acid are mixed.
8. the preparation method of a kind of biological organic fertilizer according to any one of claim 4~7, it is characterised in that including such as
Lower step：
Step (a)：Sterilized after described fowl and animal excrement and carbon source are sufficiently mixed, compound is made, then adding water will be mixed
The moisture content for closing material is adjusted to 55-65%, and fermentation material is made；
Step (b)：Composite zymocyte is inoculated in fermentation material made from step (a) and is well mixed, then at 25-35 DEG C
At a temperature of sealing and fermenting 15~30 days, obtain biological organic fertilizer.
9. the preparation method of biological organic fertilizer according to claim 8, it is characterised in that comprise the following steps：
Step (a)：Sterilized after described fowl and animal excrement and carbon source are sufficiently mixed, compound is made, then adding water will be mixed
The moisture content for closing material is adjusted to 60%, and fermentation material is made；
Step (b)：Composite zymocyte is inoculated in fermentation material made from step (a) and is well mixed, then in 30 DEG C of temperature
The lower sealing and fermenting of degree 24 days, obtains biological organic fertilizer.
10. preparation method according to claim 8 or claim 9, it is characterised in that also including step (s) drying-granulating：By step
(b) biological organic fertilizer obtained is dried, and is prepared into graininess finished product.
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| CN114317379A (en) * | 2022-01-28 | 2022-04-12 | 重庆融极浩瀚生物技术有限公司 | Complex microbial inoculant for producing high-activity cellulase as well as preparation method and application thereof |
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