CN103772011B - The liquid bacterial manure of a kind of cereal special composite biological - Google Patents

The liquid bacterial manure of a kind of cereal special composite biological Download PDF

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Publication number
CN103772011B
CN103772011B CN201310747136.4A CN201310747136A CN103772011B CN 103772011 B CN103772011 B CN 103772011B CN 201310747136 A CN201310747136 A CN 201310747136A CN 103772011 B CN103772011 B CN 103772011B
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bacterial manure
composite biological
special composite
liquid bacterial
cereal
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CN201310747136.4A
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CN103772011A (en
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乔长晟
钟海蛟
李雪
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Hunan Xinyu Biotechnology Co., Ltd.
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Tianjin Peiyang Biotrans Biotech Co Ltd
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Abstract

The liquid bacterial manure of a kind of cereal special composite biological, relates to the preparation of the liquid bacterial manure of applicable cereal special composite biological.Nutritive ingredient for the corn growing cycle is carried out reasonably combined, aseptically, pressed powder and fermented bacterium liquid mixing is obtained.The present invention can extend and keep the long-term lasting supply of fertilizer by the slowly-releasing of microorganism.Effectively can prevent disease and pest, improve soil constituent, strengthen crop resistibility, stabilizing carbon dioxide, strengthen soil fertility, stimulate plant growth.The present invention is extensively applied to cereals crop.

Description

The liquid bacterial manure of a kind of cereal special composite biological
Technical field
The invention belongs to field of compound fertilizer production, be specifically related to the preparation of the liquid bacterial manure of a kind of applicable cereal special composite biological.
Background technology
Common fertilizer is for cereals crop, and fertilizer efficiency period is short, and nutrient formula is unreasonable, supplies not science, more unbalanced.Long-term application chemical fertilizer, effectively nutritive substance can not be provided to crop, also can cause the loss of main component, cause the huge waste of resource and the increase of agricultural cost, produce the pollution in source, water and soil face, underground simultaneously, thus cause the eutrophication of greenhouse gases effect and water body, make the deterioration of the ecological environment.In such a situa-tion, carry out the reasonably combined of nutritive ingredient in the urgent need to the growth cycle for cereal, then extended and keep the long-term lasting supply of fertilizer by the slowly-releasing of microorganism.
Cereals mainly refer to the seed of grass.Five cereals mainly comprise rice, wheat, corn etc. and other coarse cereals, such as millet, black rice, buckwheat, oat, seed of Job's tears, Chinese sorghum etc.Cereal by being processed as staple food, for the mankind provide heat energy, the protein of 40% ~ 70%, the VITMAIN B1 of more than 60% of 50% ~ 80%.Cereal can because of the difference of kind, kind, the place of production, growth conditions and working method, and the content of its nutrient substance has very large difference.
Cereals, as Chinese traditional food, are one of indispensable food on common people's dining table for thousands of years always, occupy an important position in the meals of China, be taken as traditional staple food.Paddy, phytology belongs to the common rice subspecies in Gramineae Oryza common cultivated rice subgenus.It is one of topmost food crop of China, and at present, the sown area of China paddy rice accounts for 1/4 of the food crop total area, and output accounts for 1/2 of national total output of grain, accounts for over half in commodity food.Producing region is throughout all parts of the country.Common cultivated rice can be divided into long-grained nonglutinous rice and japonica rice two subspecies.Long-grained nonglutinous rice particle shape is elongated, and length is more than three times of width, and flat, fine hair is short and rare, and general without awns, even if there is awns also very short, rice husk is thinner, and white belly is comparatively large, and hard grain is less, and add and easily go out to crack rice man-hour, rice milling yield is lower, and property that rice matter is swollen is comparatively large and viscosity is more weak.Then particle shape is short cuts for japonica rice, length be the 1.4-2.9 of width doubly, fine hair is long and close, and awns is longer, and rice husk is thicker, and white belly is little or do not have, and hard grain is many, adds not easily to produce man-hour to crack rice, and rice milling yield is higher, and property that rice matter is swollen is less and viscosity is stronger.Long-grained nonglutinous rice or japonica rice, according to the difference of length in its, can be divided into again early rice, semilate rice and late rice three class in vegetative period.The 90-125 days in vegetative period of early rice, the vegetative period of semilate rice is 125-150 days, and the vegetative period of late rice is 150-180 days.Due to length and the difference of weather condition in vegetative period, the quality of the paddy of same type also shows some difference: early the general white belly of rice is comparatively large, and hard grain is less, and rice matter is loosened, inferior quality, otherwise late rice rice then, and quality is better.Wheat is a kind of temperate zone long day plant, and subject range is comparatively wide, and from north latitude 18 ° ~ 50 °, from Plain to height above sea level, the height (as Tibet, China) of 4000 meters all has cultivation.According to the spicate density degree of wheat, the structure of small ear, the proterties, color, lint etc. of lepicena, lemma and awns and grain, be divided into extremely many subspecies, mutation, modification and kind under kind; According to different to the requirement of temperature, point winter wheat and spring wheat two bions, different areas plantation is dissimilar.In China Dark Longjiang, the Inner Mongol and northwest plant spring wheat, in sowing in 3 ~ April of spring, 7 ~ August is ripe, and breeding time is short, about about 100 days; In the east area of the Liao River, coextensive with eastern and southern Liaoning Province, North China, southern Xinjiang, Shaanxi, Yangtze valley each province and south China one is with plantation winter wheat, and 8 ~ December of autumn is sowed, and 5 ~ July of next year is ripe, reaches about 300 days breeding time.The main planting system of corn: northeast, North of North China and Counties of North-west Five area are 1 year ripe Spring Maize Area; The North China Plain is based on double-cropping system summer corn; Southwest and southern hilly area with a varied topography, high and cold mountain area is with 1 year ripe spring maize, Hills with double-cropping system summer corn, and Plain and Middle hills are based on three crops per annual autumn corn.Planting type has nonoculture, or interplants with beans, the intercropping of potato class and Mai Long.Sowing date for early sowing is very important to its volume increase.According to fertilizer, water condition and breediness, rational close planting, ensures that enough seedling numbers and spike number are the keys of high yield.Corn needs nitrogen, potassium more, needs phosphorus less, and can utilize first crop phosphoric residue element, but soil available phosphorus amount lower than 10ppm time, execute phosphorus and significantly increase production.Responsive to zinc fertilizer, grow suppressed when lacking zinc.Full fertility water requirement 5600 ~ 6100 millimeters/hectare.Need seedling stage water less, jointing-booting stage increases, and take out male flowering period at most, grouting parameter is more.
Summary of the invention
The object of this invention is to provide the production technique of the liquid bacterial manure of a kind of cereal special composite biological, the nutritive ingredient for the corn growing cycle is carried out reasonably combined, is extended and keep the long-term lasting supply of fertilizer by the slowly-releasing of microorganism.
The present invention adopts following technical scheme:
Material content of the present invention (calculating by weight) is as follows:
Concrete technical process is as follows:
(1), under normal temperature aseptic condition, a certain amount of pressed powder is added in tempering tank in proportion and stirs.
(2) under normal temperature aseptic condition, by four kinds of bacteria containing amounts all 10 8the fermented bacterium liquid of individual/more than ml adds mixing and stirring in another tempering tank by a certain percentage, as the solvent of pressed powder raw material.
(3) the pressed powder screw feeder mixed in (1) step is delivered in the tempering tank that bacterium liquid is housed, mixing and stirring, by the storage of mixed sample sealing normal temperature lucifuge.
Described bacillus thuringiensis, bacillus megaterium, Pseudomonas fluorescens and Streptomyces jingyangensis are obtain zymocyte liquid after fermentation culture.
Beneficial effect
The present invention utilizes the material actings in conjunction such as microorganism, inorganic salt, organism, long-lastingly can provide nutritive substance to reach to cereal.The microorganism that well-chosen is suitable, one of its bacterial classification: bacillus thuringiensis is a kind of microbial source low toxic pesticide, based on stomach poison function, can effectively preventing insect pest, and to people and animals without action effect.Bacterial classification two: bacillus megaterium, can self phosphorus decomposing fixed nitrogen, organophosphorus in soil of can well degrading, and for plant provides required nutrition nutrient, improves soil constituent.Bacterial classification three: colloid bacillus cereus, also known as silicate bacteria, can secrete plant growth substance and multiple enzyme, to strengthen the resistibility of crop to some diseases.Promote the conversion of soil inavailable phosphorus potassium, increase the supply of soil phosphorus potassium, improve crop yield, the intensity of colloid bacillus cereus phosphorus decomposing, potassium decomposing, solution silicon is very good especially simultaneously.Colloid bacillus cereus also can produce carbonic anhydrase, has certain effect to the fixing tool of carbonic acid gas.Bacterial classification four: Streptomyces jingyangensis, can suppress some fungal diseases.There is the ability strengthening soil fertility, stimulate plant growth.By reasonably adjusting proportioning to their different-effect, acting synergistically between each bacterial classification, the function of bacterial classification farthest being played, with the demand of applicable corn growing.
Embodiment
Cereal composite biological fertilizer specially formula (calculating by weight, unit: part) is as follows:
Raw material Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4
The fertile EDTA-Ca of Ca 6.2 6.5 6.8 7.2
The fertile EDTA-Mn of Mn 0.15 0.18 0.21 0.25
The fertile EDTA-Cu of Cu 0.015 0.017 0.019 0.02
The fertile EDTA-Zn of Zn 0.042 0.045 0.048 0.051
The fertile EDTA-Mg of Mg 7.25 7.45 7.85 8.32
The fertile EDTA-Fe of Fe 0.15 0.18 0.21 0.24
Zinc Sulphate Heptahydrate 0.028 0.03 0.032 0.035
Magnesium sulfate heptahydrate 4.5 4.8 5.0 5.2
Manganese sulfate monohydrate 0028 003 0032 0035
Cupric sulfate pentahydrate 0.018 0.02 0.022 0.025
Boric acid 0.045 0.048 0.05 0.052
Sodium alginate 0012 0016 002 0025
Ammonium molybdate 0.022 0.023 0.026 0.028
Potassium primary phosphate 72 73 74 75
Humic acids 0.22 0.24 0.26 0.28
Soybean protein 1.15 1.28 1.4 1.5
Asparagus juice 2.25 2.3 2.4 2.5
Seaweed powder 0.32 0.38 0.46 0.55
Water glass 0.022 0.026 0.03 0.035
Anhydrous slufuric acid is ferrous 0.12 0.14 0.16 0.18
Bacillus thuringiensis 43.2 45.5 47.5 48.6
Bacillus megaterium 22.5 23.1 23.7 24.3
Colloid bacillus cereus 22.5 23.1 23.7 24.3
Streptomyces jingyangensis 2.83 3.5 4.2 4.82
Wherein, bacterial classification of the present invention is as follows:
(1) bacillus thuringiensis: bacillus thuringiensis (Bacillusthuringiensis is called for short Bt) is the biotic pesticide that current production rate is maximum, use is the widest.To insects such as lepidopteran, Coleoptera, Diptera, Hymenoptera, Homopteras, and the arthropods such as animals and plants nematode, acarian has specific cytotoxicity, and to nontarget organism safety therefore, has single-minded, efficient and to advantages such as person poultry safeties.Culture presevation is numbered: Guangdong Province's Culture Collection, numbering GIM1.151.
(2) bacillus megaterium: bacillus megaterium has effect of organophosphorus in soil of well degrading, is the conventional bacterial classification of widely used production biological organic fertilizer in the world, it is unique to raising tobacco fermentation flavouring effect it to be administered on tobacco leaf.Culture presevation is numbered: China General Microbiological culture presevation administrative center, numbering CGMCC1.223.
(3) colloid bacillus cereus: colloid bacillus cereus is also known as silicate bacteria, its key property is potassium, the silicon that can decomposite in the mineral such as feldspar, mica, also the phosphorus in phosphatic rock can be decomposited, and secrete plant growth substance and multiple enzyme, to strengthen the resistibility of crop to some diseases, be the best bacterial classification being used as bio-feritlizer at present, the conversion of soil inavailable phosphorus potassium can be promoted, increase the supply of soil phosphorus potassium, improve crop yield.It can produce carbonic anhydrase simultaneously, has certain effect to the fixing tool of carbonic acid gas.Culture presevation is numbered: Chinese industrial Microbiological Culture Collection administrative center, numbering CICC23575.
(4) Streptomyces jingyangensis: some fungal diseases can be suppressed.There is the ability strengthening soil fertility, stimulate plant growth.Culture presevation is numbered: Guangdong Province's Culture Collection, numbering GIM4.92.
Embodiment 1
(1), under normal temperature aseptic condition, pressed powder is added in tempering tank in proportion and stirs.
(2) under normal temperature aseptic condition, by four kinds of bacteria containing amounts all 10 8the fermented bacterium liquid proportional of individual/more than ml adds mixing and stirring in another tempering tank, as the solvent of pressed powder raw material.
(3) the pressed powder screw feeder mixed in (1) step is delivered in the tempering tank that bacterium liquid is housed, mixing and stirring, by the storage of mixed sample sealing normal temperature lucifuge.
Embodiment 2
(1), under normal temperature aseptic condition, pressed powder is added in tempering tank in proportion and stirs.
(2) under normal temperature aseptic condition, by four kinds of bacteria containing amounts all 10 8the fermented bacterium liquid proportional of individual/more than ml adds mixing and stirring in another tempering tank, as the solvent of pressed powder raw material.
(3) the pressed powder screw feeder mixed in (1) step is delivered in the tempering tank that bacterium liquid is housed, mixing and stirring, by the storage of mixed sample sealing normal temperature lucifuge.
Embodiment 3
(1), under normal temperature aseptic condition, pressed powder is added in tempering tank in proportion and stirs.
(2) under normal temperature aseptic condition, by four kinds of bacteria containing amounts all 10 8the fermented bacterium liquid proportional of individual/more than ml adds mixing and stirring in another tempering tank, as the solvent of pressed powder raw material.
(3) the pressed powder screw feeder mixed in (1) step is delivered in the tempering tank that bacterium liquid is housed, mixing and stirring, by the storage of mixed sample sealing normal temperature lucifuge.
Embodiment 4
(1), under normal temperature aseptic condition, pressed powder is added in tempering tank in proportion and stirs.
(2) under normal temperature aseptic condition, by four kinds of bacteria containing amounts all 10 8the fermented bacterium liquid proportional of individual/more than ml adds mixing and stirring in another tempering tank, as the solvent of pressed powder raw material.
(3) the pressed powder screw feeder mixed in (1) step is delivered in the tempering tank that bacterium liquid is housed, mixing and stirring, by the storage of mixed sample sealing normal temperature lucifuge.
Field experiment
The liquid bacterial manure of cereal special composite biological prepared by the present invention and common crop of applying fertilizer contrast, crop yield successful, concrete testing program and result as follows:
Embodiment 1 gets composite biological fertilizer 2ml of the present invention, dilute 500 times, spray on the millet seedling just sprouted, every square meter sprays the composite bio-fertilizer of the present invention of 1 ~ 1.5L amount, treats that stem and leaf of Wheat grows to 10 ~ 15cm, refertilizes once, this time every square meter sprays the composite fertilizer of 2 ~ 2.5L amount, then wait during wheat heading and execute last fertilizer, every square meter fertilising 1 ~ 1.5L amount, this time adopts the mode of filling with root to apply fertilizer to it.With the stem and leaf of Wheat ratio not executing this composite fertilizer, in whole growth cycle, the seedling of fertilising is more sturdy than common, and color is greener, and heading period is advanced by 2 ~ 3 days, and average every square meter can improve the effect of increasing production of 8%.
Embodiment 2 gets composite biological fertilizer 2ml of the present invention, dilute 500 times, spray on the maize seedling just sprouted, every square meter sprays the composite bio-fertilizer of the present invention of 1 ~ 1.5L amount, treats that maize seedling grows to 30 ~ 35cm, refertilizes once, this time every square meter sprays the composite fertilizer of 2 ~ 2.5L amount, then wait during corn heading and execute last fertilizer, every square meter fertilising 1 ~ 1.5L amount, adopts the mode of filling with root.With the maize seedling ratio not executing this composite fertilizer, in whole growth cycle, the seedling of fertilising is more solid than common seedling stalk, color is greener, the fruit of knot is than common length 2 ~ 3cm, and growth cycle shortens 6 ~ 8 days than unmanured, and the average every square meter of fruit harvest can improve the effect of increasing production of 10%.
Embodiment 3 gets composite biological fertilizer 2ml of the present invention, dilute 500 times, spray on the oat seedling just sprouted, every square meter sprays the composite bio-fertilizer of the present invention of 1 ~ 1.5L amount, treats that oat seedling grows to 20 ~ 325cm, refertilizes once, this time every square meter sprays the composite fertilizer of 2 ~ 2.5L amount, execute last fertilizer when then waiting oat to start to ear, every square meter fertilising 1 ~ 1.5L amount, adopts and fills with root mode.With the oat seedling ratio not executing this composite bio-fertilizer, in whole growth cycle, the seedling of fertilising is more solid than common seedling stalk, the unmanured many heading of every young plant average specific 5 ~ 6 wheat heads, fruit is fuller, every square meter output increased effect of 11%.

Claims (4)

1. the liquid bacterial manure of cereal special composite biological, it is characterized in that, formula number is counted:
Described bacillus thuringiensis (Bacillusthuringiensis) is numbered GIM1.151; Described bacillus megaterium (Bacillusmegaterium) is numbered CGMCC1.223; Described colloid bacillus cereus (Bacillusmucilaginosus) numbering CICC23575; Streptomyces jingyangensis (StreptomycesjingyangensisTaoetal) numbering GIM4.92.
2. the liquid bacterial manure of cereal special composite biological according to claim 1, is characterized in that, adopt following formula number to count:
3. the liquid bacterial manure of cereal special composite biological according to claim 1, is characterized in that, bacterial content>=10 in described each bacterium bacterium liquid 8individual/mL.
4., according to the using method of the liquid bacterial manure of the arbitrary described cereal special composite biological of claim 1-3, it is characterized in that:
Get the liquid bacterial manure 2mL of compound bio, dilute 500 times, spray on the oat seedling just sprouted, every square meter sprays 1 ~ 1.5L amount, treats that oat seedling grows to 20 ~ 325cm, refertilizes once, this time every square meter sprays 2 ~ 2.5L amount, execute last fertilizer when starting to ear, every square meter fertilising 1 ~ 1.5L amount, adopts and fills with root mode.
CN201310747136.4A 2013-12-27 2013-12-27 The liquid bacterial manure of a kind of cereal special composite biological Expired - Fee Related CN103772011B (en)

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CN106699416A (en) * 2017-02-23 2017-05-24 胡世洋 Multielement liquid compound microorganism fertilizer and preparation method thereof
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CN107827627A (en) * 2017-12-26 2018-03-23 天津北洋百川生物技术有限公司 A kind of strawberry special composite biological fertilizer and its preparation method and application
CN108401636A (en) * 2018-04-19 2018-08-17 中国农业大学 A kind of high-yield fertilization method of organic oat
CN110622795A (en) * 2019-09-27 2019-12-31 贵州省亚热带作物研究所 Good agricultural operation management method for coix

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CN1150133A (en) * 1996-09-20 1997-05-21 北京大学 Efficient biological compound fertilizer and its preparing process
CN101054568A (en) * 2007-04-17 2007-10-17 周文彩 Microorganism bacterium composition, special biological organic fertilizer for tobacco containing the same, preparation and use method for the microorganism bacterium composition
AU2009200226A1 (en) * 2008-01-25 2009-08-13 John Pashai Improved Fertiliser
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