CN106399157A - Microbial agent with facility agricultural soil remediation function, and preparation and application of microbial agent - Google Patents

Microbial agent with facility agricultural soil remediation function, and preparation and application of microbial agent Download PDF

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Publication number
CN106399157A
CN106399157A CN201610784626.5A CN201610784626A CN106399157A CN 106399157 A CN106399157 A CN 106399157A CN 201610784626 A CN201610784626 A CN 201610784626A CN 106399157 A CN106399157 A CN 106399157A
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China
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repair function
facility agricultural
agricultural soil
bacterial agent
microbial bacterial
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闫龙翔
阚雨晨
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LULE BIOLOGICAL SCIENCE AND TECHNOLOGY Co Ltd SHANGHAI
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LULE BIOLOGICAL SCIENCE AND TECHNOLOGY Co Ltd SHANGHAI
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B09DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09CRECLAMATION OF CONTAMINATED SOIL
    • B09C1/00Reclamation of contaminated soil
    • B09C1/10Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B09DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09CRECLAMATION OF CONTAMINATED SOIL
    • B09C2101/00In situ

Abstract

The invention discloses a microbial agent with a facility agricultural soil remediation function, and preparation and application of the microbial agent. The method comprises bacterial fermentation and adsorption. According to the method, a bacterial liquid after fermentation of bacillus megaterium ACCC10010 is adsorbed according to a mass ratio of a bacterial liquid to hydrolysis residues of 0.3 to (2-4) by using the hydrolysis residues after treatment as a bacterial liquid adsorption medium, and mixing and grinding are performed to obtain the microbial agent with the facility agricultural soil remediation function. The microbial agent with the facility agricultural soil remediation function, provided by the invention, can effectively reduce nitrate nitrogen content in secondary salinity soil and greatly increase a microbial population quantity and plant yield; a method for remedying the secondary salinity soil by adopting the microbial agent belongs to in-situ remediation and is simple in operation, low in cost and easy for industrial implementation; and the remediation effect is good, the soil fertility is not destroyed, and the resource utilization rate can be increased.

Description

Facility agricultural soil repair function microbial bacterial agent and its preparation, purposes
Technical field
The present invention relates to soil microorganism technology repairs field, particularly to a kind of micro- life of facility agricultural soil repair function Thing microbial inoculum and its preparation, purposes.
Background technology
With the expansion of facility cultivation area, the usage amount of chemical nitrogen fertilizer significantly rises, and substantially exceeds the demand of plant Amount, the nitrate due to plant absorption can not reduce assimilation in time, and the accumulation in plant body is increasingly serious, plant absorption simultaneously A large amount of salinities not are accumulated in soil, cause soil secondary salinization, wherein based on the accumulation of nitrate.
Environmentally, the accumulation of N element, can cause the waste of fertilizer and affect plant growth and soil profit With can pass through rainwash and the farmland seepage in farmland, lead to widespread pollution from the overuse of fertilizers and pesticides in rural area simultaneously.For food safety angle, people After the nitrate of body excess intake, nitrate (NO in vivo3) easily it is reduced into nitrite (NO2-), nitrite can make carefully In born of the same parents the low Ferri-hemoglobin of oxygen carrying be oxidized to no the high ferritin of oxygen carrying capacity and cause tissue lack, moreover, oxidized The high ferritin becoming also can reduce the function that the HbO2 Oxyhemoglobins having carried oxygen discharge oxygen to bodily tissue, makes human body anoxia, Have, when serious, the danger making one death by suffocation.More frighteningly nitrous can be generated after nitrite and dimethylamine, trimethylamine effect Amine.Nitrosamine is a kind of compound with strong carcinogenecity, and in 120 kinds of nitrosamine compounds having been found that, 75 is true Recognizing has carcinogenecity, and nitrosamine also causes the potential danger of hereditary variation and monster, once this material enters vegetable, to people's Impact is very fearful.But the azotate pollution of victual is quite serious and universal, thus mistake in treatment facility cultivation Amount nitrate nitrogen oneself through becoming one of industrialized agriculture important and instant work.China's facility cultivation booth salt at present Stainization harm takes some treatment measures, such as washing salinity by irrigation method, the method such as soil conditioner method and semi-decomposed fertilizer method, though So these methods have the advantages that many, but there is also very big deficiency:After washing salinity by irrigation method makes nitrate nitrogen be leached subsoil water, Not only cause the loss of soil nitrogen, but also pollute subsoil water;Soil conditioner method cost is higher, it is possible to produce Secondary pollution;Semi-decomposed fertilizer method fertilizer efficiency is slow, in-convenience in use the problems such as.
Compared with traditional process secondary salinization method, microorganism remediation method has the advantages that many is prominent, does not produce two Secondary pollution, does not increase surrounding burden, and low cost is easy to operate.Nitre in secondary salinization soil is processed using microbial method One of key of state nitrogen is to filter out to have the efficient strain excellent assimilating nitrate nitrogen ability.Microorganism is equal to each pollutant Have relatively strong, adaptability faster, ratio can degrade, assimilate as metabolism atural object, safety good it is easy to manage.With me The becoming increasingly conspicuous of the expansion of state's facility cultivation area and secondary salinization problem, microbial method processes research and the application of nitrate nitrogen There is wide prospect.
Bio-feritlizer is the product containing specified microorganisms live body, is applied to agricultural production, by microorganism contained by it Vital movement, increases the supply of plant nutrient or promotes plant growing, improve yield, improve quality of agricultural product and Agro-ecology Environment.According to the requirement of Ministry of Agriculture's agricultural microbial agent industry standard (GB 20287-2006), living bacteria count amount must be big In 2 × 108Individual/gram, the high microbial bacterial agent product of number of viable, can not only improve product using effect, also can substantially reduce Composite microbiological fertilizer and the production cost of bio-organic fertilizer, improve the market competitiveness of product.However, microbial inoculum to be improved The quantity of middle viable bacteria, the size of the adsorbance to bacterium solution fermentation liquid for the absorption carrier, and whether be conducive to viable bacteria survival and multiply It is the key of impact number of viable.At present, the generation of microbial bacterial agent is mainly restricted by factor once.
1st, by the use of turf or humic acidss as organic carrier, resource, indissoluble solution are consumed:Contain a large amount of in turf and humic acidss Organic matter, C/N, than the conditions of existence meeting microorganism, ensure that the number of viable of microorganism.But turf and humic acidss belong to In mineral, limited by national resources, a large amount of exploitations can destroy ecology, and apply and be difficult to dissolving in soil, therein had Machine matter and humic acidss are difficult to, by crop utilization, affect fertilizer efficiency, and the price of turf and humic acidss also goes up year by year simultaneously, the life of product Produce cost also improving year by year.
2nd, moisture is high, and number of viable is few:Carry out the R&D institution of microbial bacterial agent production and enterprise substantially all with grass Charcoal, brown coal, weathered coal are as absorption carrier, but the water content in these materials is very high, even if carrying out high temperature to dry therein containing The water yield also in 15-20%, the water content of general finished product microbial inoculum 35% about, so the adsorbance of bacterium solution is in 10-15%, if , in 25-30 hundred million/gram, after absorption, the number of viable of microbial inoculum can only be in 2.5-4.5 hundred million/gram for the number of viable of bacterium solution.
3rd, no nutrition, the viable bacteria time-to-live is short:In turf and the differentiation material such as coal and do not contain the nutrient needed for viable bacteria Matter, by complex microorganism bacterium solution direct inoculation on the carriers such as turf, differentiation coal, rice husk, As time goes on, number of viable To gradually decrease, the resting period is limited.
Content of the invention
Therefore, the technical problem to be solved in the present invention is, is to overcome the shortcomings of that above-mentioned prior art exists, provides one kind Facility agricultural soil repair function microbial bacterial agent.The invention solves the problems that another technical problem be to provide the system of this microbial inoculum Preparation Method, and the purposes of this microbial inoculum is provided.
In a first aspect, the technical scheme is that, a kind of facility agricultural soil repair function microbial bacterial agent, facility agriculture After industry soil remediation functional microorganism microbial inoculum includes bacillus megaterium (Bacillus megaterium) ACCC10010 fermentation Bacterium solution and amino acids production process hydrolysis residue, the mass ratio of bacterium solution and hydrolysis residue is 0.3-0.6:2-4, viable count is 2-10 Hundred million/gram.The present invention relates to a kind of facility agricultural soil repair function microbial bacterial agent, described facility agricultural soil repair function is micro- Bacteria agent is made up of Bacillus megaterium (Bacillus megaterium) ACCC10010 bacterium solution and carrier, and described carrier is Acid hydrolysis slag.
The deposit number of described bacillus megaterium is ACCC10010, and Classification And Nomenclature is bacillus megaterium (Bacillus megaterium), is saved in Chinese agriculture Microbiological Culture Collection administrative center (address:Haidian District, Beijing City Zhong Guan-cun South Street 12;Postcode 100081), buied by commercial sources.Chinese patent 200710037760.X,《A kind of huge The cultural method of Bacterium anthracoides and its application in processing municipal sludge》Also used bacillus megaterium (Bacillus megaterium)ACCC10010.
Preferably, the cultural method of bacillus megaterium ACCC10010 is as follows:By bacillus megaterium ACCC10010 Strain accesses in YPD culture medium and is cultivated, and cultivation temperature is set as 29-32 DEG C, and shaking speed is 230-260r/min.Described YPD culture medium is the mixed aqueous solution of following raw material:Peptone 18-23g/L, yeast powder 18-22g/L, glucose 8-12g/L, Agar 15-17g/L, the initial pH of this YPD culture medium is 7.0~8.0.
It is further preferred that in above-mentioned cultural method, cultivation temperature is set as 30 DEG C, shaking speed is 250r/min.Described YPD culture medium is the mixed aqueous solution of following raw material:Peptone 20g/L, yeast powder 20g/L, glucose 10g/L, agar 16g/ L, the initial pH of this YPD culture medium is 7.0~8.0.
Second aspect, present invention also offers a kind of preparation method of facility agricultural soil repair function microbial bacterial agent, Preferably, the method comprising the steps of:
(1) strain fermentation:Bacillus megaterium (Bacillus megaterium) ACCC10010 is utilized Zymolysis Equipment Fermenting and producing, the bacterium solution after being fermented;
(2) process of hydrolysis residue:Hydrolysis residue is broken into into pieces powder or little particle, mixes with powdered lime, pulverize, powder is thin Stockpile put and allow it fully to react so as to pH reaches 5.5-8.5;
(3) preparation of microbial inoculum:Add bacterium solution in the hydrolysis residue that step (2) obtains, by the mass ratio of bacterium solution and hydrolysis residue It is 0.3-0.6:The amount of 2-4 is adsorbed, mix homogeneously;
(4) more mixed microbial inoculum is pulverized, obtain finished product facility agricultural soil repair function microbial bacterial agent.
In another embodiment, after step (2) compound fully reacts, carry out second pulverizing.The present invention A kind of method be in step (2) hydrolysis residue and Calx hybrid reaction, make its pH reach 5.5-8.5 after, directly bacterium solution is inhaled Attached, pulverized again after absorption;And second method is in step (2) hydrolysis residue and Calx hybrid reaction, so that its pH is reached After 5.5-8.5, first once pulverized, then bacterium solution is adsorbed, pulverized again after absorption.That is, first method is total Experience is pulverized twice altogether, and second method experiences three pulverizing.
The production method of the facility agricultural soil repair function microbial bacterial agent according to the present invention is it is preferred that in step (2), after compound fully reacts, carry out second pulverizing.
The production method of the facility agricultural soil repair function microbial bacterial agent according to the present invention is it is preferred that described water Solution slag:The mass ratio of Calx is 4-5:1;Or, described hydrolysis residue and lime pile put back the time answered for 12-20 hour.
The determination of the mass ratio of hydrolysis residue and Calx is that the hydrolysis residue pH after making final process reaches 5.5-8.5.Hydrolysis residue Putting back the time answered with lime pile is also not particularly limited so as to react fully, temperature drops to room temperature, preferably reaction when Between be 12-20 hour.
The production method of the facility agricultural soil repair function microbial bacterial agent according to the present invention is it is preferred that described step Suddenly the microbial inoculum moisture Control after absorption in (3), within 35%, is defined by loose caking.
The production method of the facility agricultural soil repair function microbial bacterial agent according to the present invention is it is preferred that step (1) In described bacterium solution, total living bacteria count reaches 35-60 hundred million/ml bacterium solution;Described finished product facility agricultural soil repair function microbial bacteria The number of viable of agent be 2-10 hundred million/gram.
The Elemental characters of hydrolysis residue after treatment are as follows:
Table 1:The Elemental characters of hydrolysis residue (powdery) after process
Product quality inspection:According to the requirement of Ministry of Agriculture's agricultural microbial agent industry standard (GB 20287-2006), have Effect viable count is the key technical indexes of this product, and living bacteria count amount have to be larger than 2 × 108Individual/gram, miscellaneous bacteria rate≤15%, PH value is 5.5-8.5, proves through substantial amounts of different tests, the facility agricultural soil being produced as absorption carrier by the use of hydrolysis residue Its technical specification of repair function microbial bacterial agent can reach following index, and content of beary metal is not above Ministry of Agriculture's bio-feritlizer The innoxious index of industry standard.
Table 2:The technical specification of the facility agricultural soil repair function microbial bacterial agent being produced as carrier using hydrolysis residue
Above-mentioned/gram refers to the number containing microorganism in every gram of solid;% refer to parts per hundred parts in the solid of quality plus Enter and the mass fraction containing object.
The third aspect, present invention also offers above-mentioned facility agricultural soil repair function microbial bacterial agent repairing is given birth to again Repair secondary salinization soil described in purposes in salinization soil to be specially:By micro- for described facility agricultural soil repair function life Thing microbial inoculum and conventional fertilizer application mix and impose in secondary salinization soil topsoil, apply as base manure, mix fertile full field in site preparation Spread fertilizer over the fields, spread manuer in holes or ditch spread, repairing efficiency is plant growing cycle.
Above-mentioned facility agricultural soil repair function microbial bacterial agent can also be as administration of topdressing, by conventional fertilizer application and microbial inoculum Combine, facility agricultural soil repair function microbial bacterial agent and conventional fertilizer application are also that mixing imposes on secondary salinization soil table In layer soil.
It is preferred that described facility agricultural soil repair function microbial bacterial agent is concrete when granting in such use For:The facility agricultural soil repair function microbial bacterial agent 5-7kg that the secondary salinization soil of every mu of area of leaf vegetabless is applied, eggplant The facility agricultural soil repair function microbial bacterial agent 6-10kg that the secondary salinization soil of every mu of area of fruit is applied;Every gram institute The number of viable stating facility agricultural soil repair function microbial bacterial agent is 2-10 hundred million.
Preferably, described facility agricultural soil repair function microbial bacterial agent is mixed fertilizer with compound fertilizer and need to be used while allocating, and keeps away Exempt to shelve impact bacterial strain effect for a long time.
It is preferred that described secondary salinization soil is facility plastic greenhouse soil in such use, canopy temperature reaches 15 More than DEG C.
Purposes is exactly directly to grant soil, and described soil to be repaired need not any be processed.Mainly lean on huge spore bar Fungi degradation salt.
The know-why of the present invention is:Produce the hydrolysis residue producing during monosodium glutamate and contain substantial amounts of organic matter, aminoacid, rotten plant Acid, protein and middle trace element, in these materials, most of nutrient substance is nutrient needed for growth of microorganism, can promote fertilizer Fast-propagation in soil for the beneficial microbe in material, and the small molecule organic matter in hydrolysis residue is dissolvable in water water, for making While thing provides nutrition, promotes plant growth, and energy is quick and soil particle combines to form soil granular, and improved soil is tied Structure, makes soil become soft, ventilative, water conservation, fertilizer conservation, effectively improves crop yield.With hydrolysis residue as production facility agricultural soil The organic carrier raw material of earth repair function microbial bacterial agent, moreover it is possible to provide while ensureing the living environment of absorption microorganism Multiple nutrients that microorganism needs, while increasing fertilizer efficiency, also substantially increase the product quality of composite bacteria agent capable, directly by bacterium Plant and be seeded on hydrolysis residue, not only reduce the utilization of the resources such as turf, differentiation coal, shorten technological process, in hydrolysis residue originally The nutrient substance of body is conducive to existence and the breeding of active bacteria, with without viable count after the inoculation of nutrient substance thing before solving Postpone the phenomenon gradually decreasing in time, be conducive to extending the resting period.
Bacillus megaterium not only can reduce content and the EC value of soil nitrate-N, and can increase the soil organism Content, improve vegetable yields and quality.
Described bacillus megaterium belongs to gram positive bacteria, elongated rod shape, aerobic, motion.Reducible nitrate and Asia Nitrate.In the case of no growth factor, can be rised in value with ammonium salt, nitrate, glucose for unique carbon source.
The mass ratio of bacterium solution and hydrolysis residue is controlled in 0.3-0.6:2-4, such setting is preferred by Case Experiments On A Data out, mainly in the culture presevation time, produces bacterium number rate, product viable count and this several respect of nutrient consider, not right Solution salt has direct impact.
The present invention compensate for deficiency of the prior art, is not only found that a kind of powder facility agricultural soil repair function is micro- Bacteria agent absorption carrier, this carrier contains the various nutrient substance of growth of microorganism, is conducive to the flourish of microorganism, And solve the early stage treatment technology of this raw material.The present invention is also by repetition test it is determined that this carrier and strain Ratio of adsorption, is more beneficial for the flourish of microorganism it is ensured that its high viable count.Because cost of material is cheap and easily-available, product valency Lattice are also relatively cheap, can dissolve again, discharge viable bacteria rapidly, make the using effect of product fast and obvious, directly adsorbed with raw material Thalline decreases technological process, shortens the production time, makes the waste residue of Gourmet Powder Factory reach disposal of resources and effectively utilizes simultaneously, becomes Waste be changed into values, makes the Environmental-protection agricultural facility agricultural soil repair function microbial bacterial agent of high-quality, decreases the pollution to environment, Realize refuse reclamation.
Compared with prior art, the present invention has the advantages that:
1st, provide a kind of new absorption carrier raw material for the production of microbial bacterial agent in bio-feritlizer, break tradition Use turf, differentiation coal as bacterium solution absorption carrier during production facility agricultural soil repair function microbial bacterial agent pattern, and Solve the problems, such as the production technology using this raw material, also a large amount of high pollution waste disposal for producing in glutamic acid production process Provide a brand-new disposal of resources approach;While reducing production cost, product quality is also improved;
2nd, safe and environment-friendly:This technology direct material is the hydrolysis residue of generation during production glutamic acid, eliminates discharge dirty Dye has good economic benefit because obtaining product again, and improvement is thorough, blowdown is zero, changing waste into resources, protects ecological environment;
3rd, produce and stack, be conducive to growth to breed:Directly adsorb the brand-new adsorption method of bacterium solution using hydrolysis residue, During pulverizing after bacterium solution absorption, raw material can produce certain heat makes microbial inoculum temperature raise, in enriching that hydrolysis residue contains in itself Nutrient substance under conditions of, microbes continued growth therein breeding, increased micro organism quantity in microbial inoculum product, enter one Step improves the quality of microbial inoculum;
4th, can dissolve, fertilizer efficiency is fast:With producing the micro- life of aminoacid remaining hydrolysis residue production facility agricultural soil repair function Thing microbial inoculum, wherein contains substantial amounts of aminoacid, solubility small molecule organic matter, trace element, and aminoacid can be each of plant Individual organ directly absorbs, and the beneficial microbe in product can obtain a lot of nutrition from amino acid fertilizer, fast in soil Speed breeding, product can be observed positive effect after applying in a short time;
5th, raw material be easy to get, process is simple, the industrialized implementation that is easy to, be not subject to resource limit, investment cost reduces:Due to being useless Thing utilizes, therefore material is easy to get cheaply, can be produced in enormous quantities.Can be wasted natural resources with turf, differentiation coal, exploitation destroys plants Quilt, and insoluble, and fertilizer efficiency is low.
7th, the present invention is used for carrying out microorganism remediation to secondary salinization soil, and repairing effect is good, does not destroy soil fertility Improve agricultural abandoned goods and materials source utilization rate, recycling economy.
Brief description
Fig. 1 is that hydrolysis residue generates process chart.
Fig. 2 is hydrolysis residue processing technological flow figure.
Fig. 3 is the generation process chart of facility agricultural soil repair function microbial bacterial agent.
Specific embodiment
Embodiment hydrolysis residue is as absorption carrier production facility agricultural soil repair function microbial bacterial agent
Embodiment 1
1st, strain fermentation
Bacillus megaterium (Bacillus megaterium) is sent out through slant culture, shaking table amplification culture, seed tank Ferment and ferment tank, the number of viable of zymocyte liquid reaches 4,600,000,000/ml;
2nd, sample, process
Hydrolysis residue takes from Henan "Lotus" gourmet powder company limited by shares.
Bulk hydrolysis residue is broken into into pieces powder or little particle, with powdered lime according to hydrolysis residue:Calx=4:1 ratio is mixed Close, pulverize, stockpile thin for powder is put after 12-20 hour, pack of then sieving is so as to pH reaches 6.0-7.8.
Table 3:The Elemental characters of hydrolysis residue (powdery) after process
3rd, adsorb
Bacillus megaterium (Bacillus megaterium) is adsorbed with the hydrolysis residue handled well, bacterium solution is being inhaled According to bacterium solution (L) in attached mixing plant:Hydrolysis residue (Kg)=0.3:4 ratio is adsorbed, and then stirs, moisture Control 35% about, it is defined by loose caking;
4th, pulverize
Microbial inoculum after absorption is pulverized with chain crusher, makes fineness reach 60-80 mesh.
Embodiment 2
By bacillus megaterium (Bacillus megaterium) individually through slant culture, shaking table amplification culture, seed Tank fermentation and ferment tank, the number of viable of the zymocyte liquid of bacillus megaterium reaches 5,300,000,000/ml;
Bulk hydrolysis residue is broken into into pieces powder or little particle, with powdered lime according to hydrolysis residue:Calx=4:1 ratio is mixed Close, pulverize, stockpile thin for powder is put after 12-20 hour, carries out second pulverizing, pack of then sieving, its pH reaches 7.5- 7.8.
By bacillus megaterium (Bacillus megaterium) bacterium solution according to bacterium solution (L):Hydrolysis residue (Kg)=0.3:4 Ratio adsorbed, then stir, moisture Control 35% about, is defined by loose caking;
Microbial inoculum after absorption is pulverized with chain crusher, makes fineness reach 60-80 mesh.
Embodiment 3
Bacillus megaterium (Bacillus megaterium) is sent out through slant culture, shaking table amplification culture, seed tank Ferment and ferment tank, the number of viable of zymocyte liquid reaches 4,600,000,000/ml;
Bulk hydrolysis residue is broken into into pieces powder or little particle, with powdered lime according to hydrolysis residue:Calx=5:1 ratio is mixed Close, pulverize, stockpile thin for powder is put after 12-20 hour, pack of then sieving, its pH reaches 5.5-7.0.
Bacillus megaterium (Bacillus megaterium) is adsorbed with the hydrolysis residue handled well, is stirred in absorption According to bacterium solution (L) in mixing equipment:Hydrolysis residue (Kg)=0.3:4 ratio is adsorbed, and then stirs, and moisture Control exists 35% about, it is defined by loose caking;
Microbial inoculum after absorption is pulverized with chain crusher, makes fineness reach 60-80 mesh.
Embodiment 4
Bacillus megaterium (Bacillus megaterium) is sent out through slant culture, shaking table amplification culture, seed tank Ferment and ferment tank, the number of viable of zymocyte liquid reaches 4,600,000,000/ml;
Bulk hydrolysis residue is broken into into pieces powder or little particle, with powdered lime according to hydrolysis residue:Calx=5:1 ratio is mixed Close, pulverize, stockpile thin for powder is put after 12-20 hour, carries out second pulverizing, pack of then sieving, its pH reaches 7.0- 7.5.
Bacillus megaterium (Bacillus megaterium) is adsorbed with the hydrolysis residue handled well respectively, is being inhaled According to bacterium solution (L) in attached mixing plant:Hydrolysis residue (Kg)=0.3:4 ratio is adsorbed, and then stirs, moisture Control 35% about, it is defined by loose caking;
Microbial inoculum after absorption is pulverized with chain crusher, makes fineness reach 60-80 mesh.
Embodiment 5
Bacillus megaterium (Bacillus megaterium) is sent out through slant culture, shaking table amplification culture, seed tank Ferment and ferment tank, the number of viable of zymocyte liquid reaches 4,600,000,000/ml;
Bulk hydrolysis residue is broken into into pieces powder or little particle, with powdered lime according to hydrolysis residue:Calx=5:1 ratio is mixed Close, pulverize, stockpile thin for powder is put after 12-20 hour, carries out second pulverizing, pack of then sieving, its pH reaches 7.0- 7.5.
Bacillus megaterium (Bacillus megaterium) is adsorbed with the hydrolysis residue handled well respectively, is being inhaled According to bacterium solution (L) in attached mixing plant:Hydrolysis residue (Kg)=0.3:3.5 ratio is adsorbed, and then stirs, moisture control System 35% about, is defined by loose caking;
Microbial inoculum after absorption is pulverized with chain crusher, makes fineness reach 60-80 mesh.
Comparative example 1 uses turf as absorption carrier production facility agricultural soil repair function microbial bacterial agent
1st, sample:Turf comes from Linfen, Shanxi;
Table 4:The Elemental characters of turf
Project Organic (%) pH Fineness (mesh) Humic acidss (%) Moisture (%) Miscellaneous bacteria rate (%)
Content 53.6 5.8 80 36.3 20.6 14.5
2nd, strain fermentation is with embodiment 1;
3rd, adsorb:Bacterium solution except single culture is being adsorbed in mixing plant according to bacterium solution (L):Turf (Kg)=1:4 ratio Outside example is adsorbed, other is ibid;
4th, mixing is with embodiment 1;
5th, pulverize with embodiment 1.
Product quality inspection
The examination and test of products is carried out according to the method for Ministry of Agriculture's agricultural microbial agent industry standard (GB 20287-2006), right With hydrolysis residue and turf (i.e. embodiment 1 and comparative example 1) as carrier adsorption facility agricultural soil repair function microbial bacteria Living bacteria count in agent is measured in different times, and data is shown in Table 5, and heavy metal is well below the row of composite microbiological fertilizer Industry standard, is not measured.
Table 5 testing result to facility agricultural soil repair function microbial bacterial agent within the different holding times
Can be seen that from the testing result of food preservation test:The preservation initial stage, using turf as the industrialized agriculture of carrier adsorption soil The quantity of effective viable bacteria at maximum up to 8.6 × 10 in earth repair function microbial bacterial agent6/ gram, the living bacteria count in embodiment In 4.5-2.7 × 106/ gram between, As time goes on, the living bacteria count in embodiment is in all in the time of first 90 days Reveal the trend of rising, 90 days afterwards living bacteria count over time slowly reduce, and the facility agriculture with turf as carrier Industry soil remediation functional microorganism microbial inoculum presents downward trend always, this is because, bacterium solution is reacted after being mixed with hydrolysis residue 30 DEG C about of temperature can be produced, after such facility agricultural soil repair function microbial bacterial agent is loaded in bag, add water The nutrient substance beneficial to microbial bacterial agent Reproduction containing in itself in solution slag, facility agricultural soil repair function microbial bacteria The living bacteria count of agent presents the trend of obvious rising, and uses and do not contain any nutrient substance in turf, and living bacteria count no increases Plus.With hydrolysis residue be carrier produce facility agricultural soil repair function microbial bacterial agent in miscellaneous bacteria quantity As time goes on Present the trend being remarkably decreased, and with miscellaneous bacteria in the facility agricultural soil repair function microbial bacterial agent that turf produces for carrier Quantity downward trend is inconspicuous.It can thus be seen that being that the facility agricultural soil repair function that carrier produces is micro- with hydrolysis residue Bacteria agent is more beneficial for survival and the breeding of living bacteria count, and the breeding of suppression miscellaneous bacteria.
Test example:
Citrullus vulgariss field test effect
Carry out field plot examination with the facility agricultural soil repair function microbial bacterial agent of hydrolysis residue and turf absorption respectively Test to verify its fertilizer efficiency.
Test process:
A:Mu is applied turf and is mixed as the facility agricultural soil repair function microbial bacterial agent 5kg+15kg fine earth of carrier Uniformly spread manuer in holes
B:Mu is applied hydrolysis residue and is mixed as the facility agricultural soil repair function microbial bacterial agent 5kg+15kg fine earth of carrier Close and uniformly spread manuer in holes
Plot area 20m2If, 3 repetitions, random district's groups arrangement.All test process all make base manure, when Citrullus vulgariss are transplanted seedlings Uniformly grant in Seedling cave, manuring late and management are identical with habits fertilization.
Secondary salinization soil is facility plastic greenhouse soil, and canopy temperature reaches more than 15 DEG C.
Data statistical approach:Data duncan's new multiple range method checks significance.
Table 6:The impact to Citrullus vulgariss agronomic shape for the different disposal
Table 6 can be seen that:Process B increase 3cm longer than process A Citrullus vulgariss main stem, physiology section position increases 0.4cm, knot melon number increases Plus 0.03/plant, single melon increases 0.29kg again.
The impact to soil nitrate-N for table 7 different disposal
Table 7 can be seen that:Process B and reach the level of notable volume increase than processing A reduction nitrate nitrogen 38%, effect.
Can be seen that from above result of the test:With the microbial bacterial agent that hydrolysis residue does carrier production, there is viable count height, miscellaneous Bacterium rate is low, the good feature of fertilizer efficiency, produces biological organic fertilizer with it and can be obviously promoted watermelon growing, improves soil secondary salinization, Effect is better than the microbial bacterial agent being produced with turf as carrier.
In sum, the microbial inoculum of the present invention is to acid hydrolysis slag as carrier, to be granted secondary salinization soil In topsoil, shallow plowing makes it mix with soil, gives appropriate water source, the microorganism in microbial inoculum can make full use of in carrier Nutrition carries out vital movement as the nitrate in carbon source and soil as nitrogen source, with the organic nitrogen in synthesising biological body, plays Efficiently reduce the effect of nitrified nitrogen in soil.After microorganism dead cracking, its internal biological organic nitrogen is discharged in soil Absorbed by crop root, reduce fertilizer application amount, improve crop yield, do not form secondary environmental pollution simultaneously.To application The repairing method of microorganism of secondary salinization soil proposed by the present invention repairs the repairing effect of secondary salinization soil, adopts simultaneously Evaluated with soil physico-chemical property, microbial diversity and plant products, soil physico-chemical property describes soil before and after reparation The change of middle nitrate nitrogen content, directly reflects repairing effect;Microbial diversity describes Soil Microorganism before and after reparation Bacterium colony change, show that soil microorganism is changed into state advantageously.Plant products reflect repairing effect indirectly.Knot Fruit shows, the repairing method of microorganism of secondary salinization soil proposed by the present invention can effectively reduce containing of nitrified nitrogen in soil Amount, increases Soil Microorganism colony number and plant products, has reached good repairing effect.
Above the specific embodiment of the present invention is described.It is to be appreciated that the invention is not limited in above-mentioned Particular implementation, those skilled in the art can make various modifications or modification within the scope of the claims, this not shadow Ring the flesh and blood of the present invention.
When the facility agricultural soil repair function microbial bacterial agent of the administration present invention repairs secondary salinization soil, add huge Big bud pole bacterium, to secondary salinization soil, makes the nitrate nitrogen content in soil bright in the case of not affecting plant growing Aobvious decline is it is possible to increase quantity and the plant products of microbial population.

Claims (10)

1. a kind of facility agricultural soil repair function microbial bacterial agent it is characterised in that:The micro- life of facility agricultural soil repair function Thing microbial inoculum includes bacterium solution and amino acids production after bacillus megaterium (Bacillus megaterium) ACCC10010 fermentation The mass ratio of the hydrolysis residue of process, bacterium solution and hydrolysis residue is 0.3-0.6:2-4, viable count be 2-10 hundred million/gram.
2. a kind of preparation method of facility agricultural soil repair function microbial bacterial agent it is characterised in that:Methods described include with Lower step:
(1) strain fermentation:Bacillus megaterium (Bacillus megaterium) ACCC10010 is utilized Zymolysis Equipment to ferment Produce, the bacterium solution after being fermented;
(2) process of hydrolysis residue:Hydrolysis residue is broken into into pieces powder or little particle, mixes with powdered lime, pulverize, by material thin for powder Stacking allows it fully to react so as to pH reaches 5.5-8.5;
(3) preparation of microbial inoculum:Add bacterium solution in the hydrolysis residue that step (2) obtains, the mass ratio by bacterium solution and hydrolysis residue is 0.3-0.6:The amount of 2-4 is adsorbed, mix homogeneously;
(4) more mixed microbial inoculum is pulverized, obtain finished product facility agricultural soil repair function microbial bacterial agent.
3. facility agricultural soil repair function microbial bacterial agent according to claim 1 production method it is characterised in that: After step (2) compound fully reacts, carry out second pulverizing.
4. facility agricultural soil repair function microbial bacterial agent according to claim 1 production method it is characterised in that: Described hydrolysis residue:The mass ratio of Calx is 4-5:1;Described hydrolysis residue and lime pile put back the time answered for 12-20 hour.
5. facility agricultural soil repair function microbial bacterial agent according to claim 1 production method it is characterised in that: Microbial inoculum moisture Control after absorption in described step (3), within 35%, is defined by loose caking.
6. facility agricultural soil repair function microbial bacterial agent according to claim 1 production method it is characterised in that: In the described bacterium solution of step (1), total living bacteria count reaches 35-60 hundred million/ml bacterium solution;Described finished product facility agricultural soil repair function The number of viable of microbial bacterial agent be 2-10 hundred million/gram.
7. the use in the multiple secondary salinization soil of the facility agricultural soil repair function microbial bacterial agent repairing described in claim 1 Way is it is characterised in that described reparation secondary salinization soil is specially:By described facility agricultural soil repair function microbial bacteria Agent and conventional fertilizer application mix and impose in secondary salinization soil topsoil, apply as base manure, mix in site preparation fertile full field spread fertilizer over the fields, Spread manuer in holes or ditch spread, repairing efficiency is plant growing cycle.
8. purposes according to claim 7 is it is characterised in that described facility agricultural soil repair function microbial bacterial agent exists It is specially when granting:The facility agricultural soil repair function microbial bacteria that the secondary salinization soil of every mu of area of leaf vegetabless is applied Agent 5-7kg, the facility agricultural soil repair function microbial bacterial agent 6- of the secondary salinization soil administration of every mu of area of solanaceous vegetables 10kg;The number of viable of every gram of described facility agricultural soil repair function microbial bacterial agent is 2-10 hundred million.
9. purposes as claimed in claim 7 it is characterised in that described facility agricultural soil repair function microbial bacterial agent with multiple Hefei is mixed fertilizer and need to be used while allocating.
10. purposes as claimed in claim 7 it is characterised in that described secondary salinization soil be facility plastic greenhouse soil, booth More than 15 DEG C of Nei Wenduda.
CN201610784626.5A 2016-08-30 2016-08-30 Microbial agent with facility agricultural soil remediation function, and preparation and application of microbial agent Pending CN106399157A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
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CN108017445A (en) * 2017-12-05 2018-05-11 菏泽金正大生态工程有限公司 A kind of reclamation of salinep-alkali soil type microbial bacterial agent and preparation method thereof
CN110628659A (en) * 2018-06-21 2019-12-31 上海交通大学 Bacillus megaterium, preparation of microbial inoculum thereof and application of bacillus megaterium in soil heavy metal remediation
CN111747786A (en) * 2020-06-09 2020-10-09 福建省南靖金穗生物科技有限公司 Compound fermentation soil remediation agent and preparation method thereof

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CN102174443A (en) * 2011-02-23 2011-09-07 上海绿乐生物科技有限公司 Production method of microbial inoculum
CN105198672A (en) * 2015-09-28 2015-12-30 湖南泰谷生物科技股份有限公司 Phosphorus- and potassium-solubilizing microorganism fertilizer and preparation method and application thereof
CN105255762A (en) * 2015-10-26 2016-01-20 唐睿 Micro-ecology preparation for soil conditioning

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CN102174443A (en) * 2011-02-23 2011-09-07 上海绿乐生物科技有限公司 Production method of microbial inoculum
CN105198672A (en) * 2015-09-28 2015-12-30 湖南泰谷生物科技股份有限公司 Phosphorus- and potassium-solubilizing microorganism fertilizer and preparation method and application thereof
CN105255762A (en) * 2015-10-26 2016-01-20 唐睿 Micro-ecology preparation for soil conditioning

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108017445A (en) * 2017-12-05 2018-05-11 菏泽金正大生态工程有限公司 A kind of reclamation of salinep-alkali soil type microbial bacterial agent and preparation method thereof
CN110628659A (en) * 2018-06-21 2019-12-31 上海交通大学 Bacillus megaterium, preparation of microbial inoculum thereof and application of bacillus megaterium in soil heavy metal remediation
CN110628659B (en) * 2018-06-21 2021-10-01 上海交通大学 Bacillus megaterium, preparation of microbial inoculum thereof and application of bacillus megaterium in soil heavy metal remediation
CN111747786A (en) * 2020-06-09 2020-10-09 福建省南靖金穗生物科技有限公司 Compound fermentation soil remediation agent and preparation method thereof

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