CN1056959C - Efficient peanut yield increasing agent and its preparation - Google Patents
Efficient peanut yield increasing agent and its preparation Download PDFInfo
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- CN1056959C CN1056959C CN94102954A CN94102954A CN1056959C CN 1056959 C CN1056959 C CN 1056959C CN 94102954 A CN94102954 A CN 94102954A CN 94102954 A CN94102954 A CN 94102954A CN 1056959 C CN1056959 C CN 1056959C
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Abstract
The present invention relates to a high-efficiency peanut yield increasing agent and a preparing method thereof. The high-efficiency peanut yield increasing agent comprises fermented liquor, various plant trace elements, plant cytokinin and an adsorbent, such as humic acid or salt thereof, etc., wherein the fermented liquor is respectively fermented and cultured by bacilli and peanut rhizobia. The high-efficiency peanut yield increasing agent can be obtained by uniformly mixing the substances in certain proportions by weight. The high-efficiency peanut yield increasing agent of the present invention has the comprehensive advantages of peanut nematodiasis prevention and control, peanut seedling season growth promotion, inversion resistance enhancement, peanut yield increase, simple and convenient application, low cost, high economic benefit, nontoxicity, harmlessness and no environmental pollution. The nematode preventing and controlling rate of the high-efficiency peanut yield increasing agent is from 21.5 to 44.4%, and the peanut yield increasing rate is from 17.1 to 25.8%.
Description
The present invention relates to efficient peanut yield increasing agent and preparation method thereof, belong to microbial bacterial agent and plant growth regulator field.
The volume increase of crops is the synthesis result that is subjected to multiple factor affecting, removes and selects improved seeds for use, rationally uses outside nitrogen, phosphorus, the potash fertilizer, also needs to supply with required constant of plant growth and trace element and carries out the extermination of disease and insect pest.The main underground pests of peanut is the peanut nematode, in the harm serious area, can make peanut underproduction 20-30%, even total crop failure fully.Now the effective ways of control peanut nematode are to use nematocide, for example use DD mixture, furans pellet, the iron gram etc. that goes out.Its shortcoming be that cost height, toxicity are big, particularly residue in said medicine in the soil and cause soil environment to pollute, chronic administration can seriously be destroyed the soil ecology balance, and is totally unfavorable to developing agricultural.
The purpose of this invention is to provide a kind of double biological control peanut eelworm harm, reduce the incidence of peanut nematodosis, can provide the peanut growth required various trace elements again, promote effective nodulation and nitrogen fixation, regulate crop metabolism, make the healthy and strong growth of Peanut Crop, improve peanut real mass and output, and be nontoxic, harmless, free of contamination multifunctional efficient peanut yield increasing agent.Reach and to prevent and treat that the peanut eelworm harm can increase production not only but also the purpose of non-environmental-pollution.
Solution of the present invention is: adopt the natural enemy of peanut nematode to carry out biological control, inoculate good peanut rhizobium simultaneously, improve the nitrogen-fixing efficiency of peanut root nodule, satisfy the required nitrogenous fertilizer of peanut self growth, use an amount of plant growth regulator and the necessary various trace elements of growth.
Efficient peanut yield increasing agent of the present invention comprises:
1. bacillus, peanut rhizobium are distinguished the mixture of the zymotic fluid of fermented and cultured, wherein said bacillus comprises: bacillus pumilus (Bacillus pumilus), bacillus subtilis (Bacillus subtilis), bacillus megaterium (Bacillus megatherium), spherical bacillus (Bacillussphaericus), bacillus licheniformis (Bacillus Licheniformis), only use is wherein a kind of, also can two or more be used in combination.Selected above-mentioned bacillus all has the effect of control peanut nematode, is the natural enemy of peanut nematode in the soil.The volume ratio of two kinds of zymotic fluids is followed successively by: 2: 1.The fermentation medium that is used for bacillus is the aqueous solution that contains soybean meal, corn flour, the female powder of feed, dipotassium hydrogen phosphate and manganese sulphate at least; The fermentation medium of peanut rhizobium (Rhizobiumarachis) is the aqueous solution that contains sucrose, feed yeast powder, soybean meal or soybean cake powder, corn flour, potassium dihydrogen phosphate, phosphoric acid hydrogen dimethylamino, magnesium sulfate, sodium chloride, calcium carbonate, ferrous sulfate, sodium molybdate, boric acid and manganese sulphate at least.In 27-33 ℃, the pH6.4-7.2 fermentation.
2. minimum plant element, comprise: the compound of zinc, boron, manganese, molybdenum, iron, cobalt, potassium and chlorine, be intended to improve effective dross rate of peanut and the nitrogenase activity of rhizobium, wherein zinc, potassium and chlorine and phytocytomine produce synergistic effect, can quicken chlorophyllous formation in several times ground, strengthen photosynthesis of plants.Can select zinc sulphate 1-6%, sodium tetraborate 1-4%, cobalt chloride 0.01-0.06%, potassium chloride 2-5%, sodium molybdate 0.03-0.2%, iron sulfate 0.05-0.3%, manganese sulphate 0.3-2% for use, wherein percentage is all percetage by weight.
3. phytocytomine, available comprising: hypoxanthine, N
6-isopentenyl gland purine, N
6-IPA, zeatin, dihydro zeatin or zeatin ribose can only be selected for use wherein a kind ofly, also can select for use wherein two or more to mix and use, and content is 5-60ppm.Selected above-mentioned phytocytomine has the acceleration plant cell division, promotes plant growth and chlorophyllous formation, regulates crop metabolism, improves the function of crop resistance against disadvantage.
4. adsorbent is used to adsorb mixed culture fermentation liquid, available comprising: humic acid or its alkali metal salt or humic acid, peat composed of rotten mosses powder, brown coal powder, kaolin, precipitated calcium carbonate powder or vermiculite power, can only select for use wherein a kind of or select for use wherein two or more to mix and use.The granularity of above-mentioned various materials is the 100-120 order, and uses after 120 ℃ of-160 ℃ of sterilizations.
The weight ratio of material among the above-mentioned 1-4 in efficient peanut yield increasing agent of the present invention is followed successively by mixed culture fermentation liquid: minimum plant element: phytocytomine: adsorbent=(25-35%): (4-10%): (5-60ppm): (65-72%), make product through mixing, pack.
Efficient peanut yield increasing agent of the present invention contains bacillus and adds up to (1-1.5) * 10
9Individual/gram, peanut rhizobium (5-8) * 10
8Individual/gram.When using, but both water distribution pourings, and also can dress seed or dress seed adds foliage-spray.
The preparation method of efficient peanut yield increasing agent of the present invention comprises:
One, microbial fermentation is cultivated:
(1) triangular flask is cultivated:
Prepare each 2-4 of nutrition bouillon media and YMA medium according to a conventional method
Rise: regulate pH7.0-7.2, be sub-packed in the triangular flask of 500 milliliters.Each
Bottled 150 milliliters of triangle, 15 pounds/inch
2The 20 fens kinds of sterilizing.
With aforesaid bacillus and peanut rhizobium kind, on superclean bench
Be seeded in respectively in the triangular flask that nutrition bouillon media and YMA medium are housed,
28-30 ℃ of temperature of control placed on the shaking table shaken cultivation 3-5 days.
(2) seeding tank fermented and cultured
1. fermentation medium preparation
In A, B, three seed fermentation jars of C, by following prescription (I)
Prepare a certain amount of medium, and its average mark is loaded on A, B fermentation tank
In, be used for the fermentation of bacillus.Press prescription (II) in the C fermentation tank,
The medium of preparation and A or B jar equivalent is used for the peanut rhizobium fermentation.
Prescription (I) (percentage by weight): soybean meal 0.5-3.0%, jade
Ground rice 0.5-2.0%, feed yeast powder 0.5-1.5%, dipotassium hydrogen phosphate
0.2-0.4%, sodium chloride 0.025-0.05%, manganese sulphate 0.001-
0.005%, other are water.PH7.0-7.2,15 pounds/inch
2Sterilization
20 clocks.
Prescription (II) (percentage by weight): sucrose 0.5-2.5%, feed ferment
Female powder 0.5-1.5%, soybean meal 1.0-2.8%, corn flour 0.25-0.7%,
Dipotassium hydrogen phosphate 0.05-0.2%, magnesium sulfate 0.05-0.1%, sodium chloride
0.05-0.1%, calcium carbonate 0.15-0.3%, ferrous sulfate 0.002%,
Sodium molybdate 0.001%, boric acid 0.001%, manganese sulphate 0.001%, all the other
Be water.PH6.8-7.0,15 pounds/inch
2Sterilized 20 minutes.
2, inoculation fermentation:
When the temperature for the treatment of the fermentation cylinder for fermentation medium drops to 28-32 ℃,
Bacillus in the triangular flask is seeded in A and the B fermentation tank peanut root
The knurl bacterium is seeded in the C fermentation tank, and inoculum concentration is (0.6-2) * 10
8Individual/Bo
Rise zymotic fluid, control pH6.4-7.0, temperature 28-32 ℃, throughput 1:
0.25-0.7) fermentation, the bacterium number is in microscope inspection A, B fermentation tank
(1-1.5) * 10
10Individual/milliliter, C fermentation tank bacterium number is (8-10) * 10
9
Individual/milliliter, stop fermentation.
When a large amount of production, can reach at the bacterium number of A, B, C fermentation tank
(1-5) * 10
8Individual/when milliliter, by same inoculation method, this fermentation
Liquid is inoculated in the production fermentation tank that a large amount of corresponding medium are housed, and with
Same condition is fermented, and ends when its bacterium number reaches above-mentioned requirements.
Two, be mixed and made into product
With the zymotic fluid in A, B, the C fermentation tank in batching is poor by volume
Mix at 1: 1: 1, the aforesaid phytocytomine that adds 5-60ppm stirs well even.
By weight the mixed culture fermentation liquid of 25-35 part, 120-160 ℃ of oven dry of 65-72 part
Sterilization, granularity is the aforementioned adsorbent powder of 100-120 purpose, 4-10 part is aforesaid
The minimum plant element is mixed into product, packs.Give birth to according to the present invention
The efficient peanut yield increasing agent that produces, every gram contains described bacillus (1-1.5)
* 10
9Individual, peanut rhizobium (5-8) * 10
8Individual.
Efficient peanut yield increasing agent of the present invention outstanding advantage compared with the prior art is, adopt the harm of the natural enemy control nematode of nematode to peanut, thereby do not need to re-use agricultural chemicals and avoided its pollution soil and peanut fruit, can regulate the metabolism of crop again, strengthen the resistance against disadvantage of crop, make the healthy and strong growth of crop, promote nodulation and nitrogen fixation, improve peanut yield, reduce the peanut production cost simultaneously.
In order to be illustrated more clearly in the present invention, exemplify following examples, but it there is not any restriction to the present invention.
Embodiment 1
A kind of efficient peanut yield increasing agent of the present invention, its composition comprises:
Through 120-150 ℃ of drying and sterilizing, granularity is 1400 kilograms in a 100-110 purpose humic acid potassium powder, 50 kilograms in zinc sulphate, 41 kilograms of sodium tetraborates, 42 kilograms in potassium chloride, 1.25 kilograms of sodium molybdates, 1.42 kilograms in iron sulfate, 0.8 kilogram of cobalt chloride, 4.20 kilograms of manganese sulphates and bacillus pumilus, bacillus subtilis, 525 kilograms in 1: 1: 1 zymotic fluid mixture of peanut rhizobium and in wherein adding 45ppm zeatin, hypoxanthine and N
6The mixture of-isopentenyl gland purine three mixed in equal amounts.Above-mentioned various material is divided in the sealed bag after evenly mixing and preserves.
Above-described bacillus also can only be used bacillus pumilus or bacillus subtilis wherein, but the volumetric mixture ratio of its zymotic fluid and peanut bacillus radicicola zymotic fluid is 2: 1.
Embodiment 2
The preparation method of efficient peanut yield increasing agent in the last example:
1. triangular flask is cultivated: three described in cultivating according to aforesaid microbial fermentation
Angle bottle seed culture medium compound method, the preparation medium.Then, will lack
Bacillus pumilus and withered grass spore-bearing bacillus are seeded in respectively nutrition gravy are housed
In the triangular flask of medium.Peanut rhizobium is seeded in YMA training is housed
Support in the triangular flask of base, cotton plug beyond the Great Wall is in 28-32 ℃, on the shaking table
Shaken cultivation 4 days.
2. in 300 liters of A, B fermentation tank, respectively prepare 175 premium on currency water culture bases, its
In contain soybean meal 2%, corn flour 1.5%, feed yeast powder 1%, phosphorus
Acid hydrogen dipotassium 0.25%, sodium chloride 0.03%, manganese sulphate 0.002%,
PH7.0.0-7.2,15 pounds/inch
2Sterilized 20 minutes.
Preparation peanut rhizobium aqueous solution medium in 300 liters of C fermentation tanks
175 liters, wherein contain sucrose 1.5%, feed yeast powder 1.0%, soybean
Powder 2.0%, corn flour 0.5%, potassium dihydrogen phosphate 0.07%, phosphoric acid hydrogen
Dipotassium 0.067%, sodium chloride 0.08%, calcium carbonate 0.2%, sulfuric acid Asia
Iron 0.002%, sodium molybdate 0.001%, boric acid 0.001%, manganese sulphate
0.001% and magnesium sulfate 0.09%.PH6.8-7.0 is in 15 pounds/inch
2
Sterilized 20 minutes.
In 28-32 ℃, with the bacillus pumilus cultivated in the triangular flask and
Withered grass bud bacillus is seeded in respectively in A and the B fermentation tank; With peanut rhizobium
Be seeded in the C fermentation tank, inoculum concentration is (1-2) * 10
8Individual/the milliliter fermentation
Liquid, control pH6.4-7.0 keeps 28-32 ℃ of temperature, throughput 1:
(0.25-0.5), ferment to A, B jar bacterium number and be (1-1.5) * 10
10Individual/
Milliliter, C jar bacterium number is (8-10) * 10
9Individual/milliliter stops.
3. the zymotic fluid in A, B, the C fermentation tank was mixed in 1: 1: 1 by volume, add
Go into 45ppm zeatin, hypoxanthine and N
6-isopentenyl gland purine equivalent
The mixture that mixes.By weight with 25.42% above-mentioned mixed culture fermentation
Liquid, 67.77% in the humic acid of 120-150 ℃ of drying and sterilizing and
6.81% minimum plant element mixes, and mixes, with moulding
Pocket packs.Wherein bacillus also can only be used bacillus pumilus
Or bacillus subtilis, but the body of its zymotic fluid and peanut root nodule zymotic fluid
Long-pending mixing ratio is 2: 1.
Efficient peanut yield increasing agent of the present invention use example:
Example one, every mu with above-mentioned efficient peanut yield increasing agent 1000 grams of the present invention, are made into 1/200 concentration of aqueous solution respectively, water in the sowing cave.Disease index control efficiency to eelworm harm is listed in table one.
Table one
| The test region | 1 | 2 | 3 | 4 | On average | |
| Disease index (%) | The pouring of 1/200 solution | 50.95 | 48.00 | 37.50 | 36.65 | 43.28 |
| Clear water | 64.90 | 75.00 | 67.50 | 50.75 | 64.53 | |
| Control efficiency (%) | 21.50 | 36.00 | 44.40 | 27.70 | 34.68 | |
Become the logarithm statistical analysis through disease index, the t value is 5.023＞t0.05=3.182,5% significance level.
Example two, on ground, the barren sandstone mountain range of soil property, every mu of usefulness 1000 grams efficient peanut yield increasing agent seed dressing of the present invention, the solution of florescence with 1/100 concentration sprays once, is white sand 1016 for trying peanut varieties.Result of the test is listed in table two.
Table two
| Handle | Cell production (kilogram) | On average (kilogram) | Folding per mu yield (kilogram) | Volume increase (%) | ||
| I | II | III | ||||
| 1. dress seed+spray | 8.87 | 9.20 | 9.06 | 9.04 | 151.00 | 25.83 |
| 2. seed dressing | 8.92 | 8.76 | 8.59 | 8.76 | 145.00 | 21.58 |
| 3. contrast (spray clear water) | 7.06 | 7.35 | 7.19 | 7.20 | 120.00 | |
Through variance analysis, F value 113.58＞F0.01=18.0 between processing, 1% utmost point significance level.
Example three, at smooth loam, every cave is with the pouring of efficient peanut yield increasing agent of the present invention 20 milliliters of 2% concentration, the florescence sprays once with 1/100 concentration solution, is the 79-266 major peanut for trying peanut varieties, result of the test is listed in table three.
Table three
| Handle | Cell production (kilogram) | On average (kilogram) | Folding per mu yield (kilogram) | Volume increase (%) | ||
| I | II | III | ||||
| 1. pouring | 8.748 | 9.831 | 10.933 | 9.831 | 328.36 | 17.08 |
| 2. water+spray | 9.749 | 9.918 | 10.092 | 9.918 | 331.26 | 18.11 |
| 3. contrast (spray clear water) | 8.434 | 8.397 | 5.360 | 8.397 | 280.46 | |
Through variance analysis, F value 6.83＞F0.05=4.76 between processing, 5% significance level.
From table one, table two, table three as can be seen, using efficient peanut yield increasing agent of the present invention, is 21.5-44.4% to the control efficiency of peanut nematode, and average out to 34.68% is 5% significance level.At barren sandstone ground volume increase 21.58-25.83%, at smooth loam ground volume increase 17.08-18.11%, every mu has a net increase of peanut 47.9-50.8 kilogram, folding per hectare volume increase peanut 718.5-762 kilogram.Illustrate and use efficient peanut yield increasing agent of the present invention, can control the incidence of peanut nematodosis effectively and significantly increase peanut yield, amount of application is few, and it is low to use cost, and non-environmental-pollution is a kind of efficient peanut yield increasing agent that develops the high-quality ecological agriculture.
Claims (6)
1, a kind of efficient peanut yield increasing agent is characterized in that, described efficient peanut yield increasing agent comprises:
(1). bacillus, peanut rhizobium be the zymotic fluid of fermented and cultured respectively, and be wherein optional
With bacillus comprise: bacillus pumilus, bacillus subtilis, huge bud
Spore bacillus, spherical bacillus or bacillus licheniformis, only usefulness is wherein a kind of,
Also optional wherein two or more are used in combination, fermentation of bacillus liquid and peanut root
The volume ratio of knurl fermented liquid is followed successively by: 2: 1;
(2). the minimum plant element comprises: zinc, boron, manganese, molybdenum, iron, cobalt, potassium and chlorine
Compound, can select zinc sulphate 1-6%, sodium tetraborate 1-4%, cobalt chloride for use
0.01-0.06%, potassium chloride 2-5%, sodium molybdate 0.03-0.2%, iron sulfate
0.05-0.3%, manganese sulphate 0.2-2%, wherein percentage is all percetage by weight;
(3). phytocytomine, available comprising: hypoxanthine, N
6-iso-amylene
Base adenine, N
6-IPA, zeatin, dihydro zeatin or corn
Plain ribose can only be selected for use wherein a kind ofly, also can select wherein two or more mixing for use
Use, content is 5-60ppm;
(4). adsorbent, available comprising: humic acid or its alkali metal salt or humic acid,
Peat composed of rotten mosses powder, brown coal powder, kaolin, precipitated calcium carbonate powder and vermiculite power, only choosing
With wherein a kind of or select for use wherein two or more to mix and use described various materials
Granularity be the 100-120 order;
The weight ratio of material in described efficient peanut yield increasing agent in above-mentioned (1)-(4) is followed successively by mixed culture fermentation liquid: minimum plant element: phytocytomine: adsorbent=(25-35%): (4-10%): (5-60ppm): (65-72%).
According to the described efficient peanut yield increasing agent of claim 1, it is characterized in that 2, described efficient peanut yield increasing agent comprises:
(1 '). the training of fermenting respectively of short and small gemma village bacterium, bacillus subtilis and peanut rhizobium
The zymotic fluid of supporting, the mixing ratio of three kinds of zymotic fluids is 1: 1: 1;
(2 '). the minimum plant element, comprise zinc sulphate 2-3%, sodium tetraborate 1.5-2.5%,
Potassium chloride 2-3.5%, sodium molybdate 0.05-0.1%, iron sulfate 0.05-0.15%,
Cobalt chloride 0.03-0.05%, manganese sulphate 0.20-1%;
(3 '). phytocytomine comprises: 40-50ppm zeatin, hypoxanthine and
N
6The mixture of-isopentenyl gland purine equivalent;
(4 '). adsorbent, through the humic acid potassium powder of 120-150 ℃ of drying and sterilizing, its granularity is
The 100-110 order;
Wherein (1 '), (2 '), (3 '), the weight mixing ratio of (4 ') is followed successively by (25-30): (6-7.5): (40-50ppm): (6.5-70.7).
According to the described efficient peanut yield increasing agent of claim 1, it is characterized in that 3, the fermentation of bacillus liquid in the described efficient peanut yield increasing agent is the zymotic fluid of bacillus pumilus or bacillus subtilis.
4, a kind of preparation method of efficient peanut yield increasing agent is characterized in that, the preparation method of described efficient peanut yield increasing agent comprises:
First, microbial fermentation is cultivated:
(a) triangular flask is cultivated:
According to conventional method preparation nutrition bouillon media and each 2-4 of YMA medium
Rising tune joint pH7.0-7.2 is sub-packed in the triangular flask of 500 milliliters; Each is three years old
Bottled 150 milliliters in angle, 15 pounds/inch
2The 20 fens kinds of sterilizing;
With bacillus, wherein available comprising: bacillus pumilus, withered grass
Bacillus, bacillus megaterium, spherical bacillus or bacillus licheniformis,
Can only select for use wherein a kind ofly, can select also that wherein two or more are used in combination,
Be seeded in the above-mentioned triangular flask that the nutrition bouillon media is housed; With the peanut root nodule
Bacterium is seeded in the above-mentioned triangular flask that YMA medium is housed, and keeps on shaking table
Temperature 28-32 ℃, shaken cultivation 3-5 days;
(b) seeding tank fermented and cultured:
(b
1). the preparation fermentation medium:
By following prescription (I), in A, B seed fermentation jar, respectively join equivalent
Medium, in C seed fermentation jar by prescription (II) preparation and A or B
The medium of jar equivalent:
Prescription (I): soybean meal 0.5-3.0%, corn flour 0.5-2.0% raises
Material dusty yeast 0.5-1.5%, dipotassium hydrogen phosphate 0.2-0.4%, sodium chloride
0.025-0.05%, manganese sulphate 0.001-0.005%, other are water,
PH7.0-7.2,15 pounds/inch
220 clocks of sterilizing are used for bacillus and send out
Ferment;
Prescription (II): sucrose 0.5-2.5%, feed yeast powder 0.5-1.5%,
Soybean meal 1.0-2.8%, corn flour 0.25-0.7%, potassium dihydrogen phosphate 0.05
0.1%, dipotassium hydrogen phosphate 0.05-0.2%, magnesium sulfate 0.05-0.1%,
Sodium chloride 0.05-0.1%, calcium carbonate 0.15-0.3%, ferrous sulfate 0.002%,
Sodium molybdate 0.001%, boric acid 0.001%, manganese sulphate 0.001%, all the other
Be water; PH6.8-7.0,15 pounds/inch
2Sterilized 20 minutes, and be used for flower
Living rhizobium fermentation;
(b
2). inoculation fermentation:
In the time of 28-32 ℃, bacillus is seeded in A, the B fermentation tank,
Peanut rhizobium is seeded in the C fermentation tank, and inoculum concentration is (0.6-2) * 10
8
Individual bacterium/milliliter zymotic fluid, control pH6.4-6.8, temperature 28-32 ℃, ventilation
Amount is 1: (0.25-0.75) fermentation, and until microscope inspection A, B fermentation tank bud
Spore bacillus bacterium number is (1-15) * 10
10Individual/milliliter, C fermentation tank peanut root
Bacterium bacterium number is (8-10) * 10
9Individual/milliliter, stop fermentation; Second, be mixed and made into product
(second
1). with the zymotic fluid in A, B, the C fermentation tank in batching is poor by volume
Mix at 1: 1: 1, and wherein add the 5-60ppm phytocytomine, optional
With comprise: zeatin, dihydro zeatin, hypoxanthine, N
6-isoamyl
Thiazolinyl adenine, N
6-IPA or zeatin ribose, only choosing
With wherein a kind of, also can select two or more to mix and use;
(second
2). with (second
1) fermentation mixed liquor and minimum plant element and adsorbent press
(25-35%): (4-10%): ratio (65-72%) is mixed, and wherein plants
The thing trace element comprises: zinc, boron, manganese, molybdenum, iron, cobalt, potassium and chlorine
Compound, can select zinc sulphate 1-6%, sodium tetraborate 1-4%, chlorine for use
Change cobalt 0.01-0.06%, potassium chloride 2-5%, sodium molybdate 0.03-0.2%,
Iron sulfate 0.05-0.3%, manganese sulphate 0.3-2%, wherein percentage is all
Percentage by weight; Described adsorbent is available to be comprised: humic acid or its alkali
Slaine or humic acid, peat composed of rotten mosses powder, brown coal powder, kaolin, lightweight
Paris white or vermiculite power can only be selected for use wherein a kind of or select wherein two kinds for use
Or multiple mixing is used; The granularity of described various materials is the 100-120 order,
(second
3). pack, after above-mentioned various materials mixing are stirred evenly, be sub-packed in sealed bag
In.
According to the preparation method of the described efficient peanut yield increasing agent of claim 4, it is characterized in that 5, the bacillus of using in the described method is bacillus pumilus and bacillus subtilis,
The medium of these two kinds of bacillus in fermentation tank is the aqueous solution that contains soybean meal 2%, corn flour 1.5%, feed yeast powder 1%, dipotassium hydrogen phosphate 0.25%, sodium chloride 0.03%, manganese sulphate 0.002%; The medium of peanut rhizobium in fermentation tank is the aqueous solution that contains sucrose 1.5%, feed yeast powder 1.0%, soybean meal 2.0%, corn flour 0.5%, potassium dihydrogen phosphate 0.07%, dipotassium hydrogen phosphate 0.067%, sodium chloride 0.08%, calcium carbonate 0.2%, ferrous sulfate 0.002%, sodium molybdate 0.001%, boric acid 0.001%, manganese sulphate 0.001% and magnesium sulfate 0.09%
After fermentation stops 1: 1: 1 by volume mixed being incorporated in of three kinds of zymotic fluids is wherein added 40-50ppm zeatin, hypoxanthine and N
6The mixture of-isopentenyl gland purine mixed in equal amounts, this mixed liquor is mixed by following weight ratio: the fermentation mixed liquor: the minimum plant element: adsorbent equals (25-30): (6-7.5): (65-70) again;
Wherein the minimum plant element comprises: zinc sulphate 2-3%, sodium tetraborate 1.5-2.5%, potassium chloride 2-3.5%, sodium molybdate 0.06%, iron sulfate 0.5-0.15%, cobalt chloride 0.03-0.05%, manganese sulphate 0.2-1%; Adsorbent is that granularity is the 100-110 order through the humic acid powder of 120-150 ℃ of oven dry, sterilization.
6, in accordance with the method for claim 4, it is characterized in that the bacillus in the described method is bacillus pumilus or bacillus subtilis.
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|---|---|---|---|
| CN94102954A CN1056959C (en) | 1994-03-28 | 1994-03-28 | Efficient peanut yield increasing agent and its preparation |
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|---|---|---|---|
| CN94102954A CN1056959C (en) | 1994-03-28 | 1994-03-28 | Efficient peanut yield increasing agent and its preparation |
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| CN1056959C true CN1056959C (en) | 2000-10-04 |
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| DK2603086T3 (en) * | 2010-08-10 | 2017-01-30 | Chr Hansen As | Nematocid composition containing bacillus subtilis and bacillus licheniformis |
| CN102038004A (en) * | 2010-10-11 | 2011-05-04 | 山东郁茏生物科技有限公司 | Composite microorganism live bacterium preparation for killing nematodes |
| CN102173924B (en) * | 2011-01-10 | 2013-03-27 | 艾应伟 | Preparation method of special biofertilizer for tobaccos |
| CN102557245B (en) * | 2011-12-26 | 2013-04-17 | 新疆德蓝股份有限公司 | Microbial promotion nutrition agent for improving zoogloea structure and activity of sludge |
| CN104046578A (en) * | 2014-04-30 | 2014-09-17 | 路域生态工程有限公司 | Preparation method of slow-growth nodule bacteria medium and medium prepared by preparation method |
| CN105884487A (en) * | 2014-10-31 | 2016-08-24 | 马振宇 | Method utilizing dry yeast powder to produce composite microbial fertilizer for increasing fruit weight, sugar content and ground temperature, coloring fruits, and improving fruit quality |
| AU2018246232B2 (en) | 2017-03-27 | 2023-12-07 | Tenfold Technologies, LLC | Methods and agricultural compositions for preventing or controlling plant diseases |
| CN109121778B (en) * | 2018-08-07 | 2022-01-28 | 云南元江大有为食品有限公司 | Extraction method of bradyrhizobium extract and application of bradyrhizobium extract in mango grafting |
| CN110092693A (en) * | 2019-05-30 | 2019-08-06 | 杜英杰 | One cultivate peanut high yield volume increase regulator |
| CN112432933B (en) * | 2019-08-26 | 2021-11-19 | 北京大学 | Ultrahigh time-space resolution imaging system and method of multi-excitation light source photoelectron microscope |
-
1994
- 1994-03-28 CN CN94102954A patent/CN1056959C/en not_active Expired - Lifetime
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| CN1095892A (en) | 1994-12-07 |
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