CN1740122A - Composite microbial fertilizer with coal gangue as matrix and its production process - Google Patents
Composite microbial fertilizer with coal gangue as matrix and its production process Download PDFInfo
- Publication number
- CN1740122A CN1740122A CNA2004100568694A CN200410056869A CN1740122A CN 1740122 A CN1740122 A CN 1740122A CN A2004100568694 A CNA2004100568694 A CN A2004100568694A CN 200410056869 A CN200410056869 A CN 200410056869A CN 1740122 A CN1740122 A CN 1740122A
- Authority
- CN
- China
- Prior art keywords
- coal gangue
- fertilizer
- matrix
- streptomyces microflavus
- bacillus cereus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Landscapes
- Fertilizers (AREA)
Abstract
The present invention belongs to the field of microbial fertilizer producing technology, and aims at eliminating pollution caused by coal gangue and developing and utilizing coal gangue resource. The present invention produces the composite microbial fertilizer with coal gangue as matrix and carrier, no-harm treated chicken manure, pig manure and other organic wastes and proper amount of chemical fertilizer and through action of streptomycete and jelly bacillus to mineralize and decompose P, K, Ca, Mg, S, Cu, Zn, Fe and Mn, organic matter and SiO2 and to convert insoluble SiO2 into soluble silicide for crop to absorb and utilize. The composite microbial fertilizer has comprehensive nutrients and stable fertilizer effect, and may be used widely for vegetable, grain crop, etc.
Description
Technical field: the invention belongs to the microbial fertilizer production technical field, relating in particular to a kind of is matrix with the coal gangue, organic waste and an amount of chemical fertilizer such as compound chicken manure, pig manure through harmless treatment, the composite microbiological fertilizer that contains elite streptomyces microflavus (Streptomyces microflavus) and colloid bacillus cereus (Bacillusmucilaginosus), particularly a kind of a large amount of microbial fertilizers that utilize colliery waste-coal gangue.
Background technology: coal gangue is the waste in coal mining and the washing process, is one of industrial solid castoff of present quantity discharged maximum, and nearly hundred million tons of annual quantity dischargeds account for about 10% of coal mine output then.Totally pile up more than 3,400,000,000 ton at present, take up an area of about more than 20 ten thousand mu, but also with about 1.3 hundred million tons speed increase in every year.Coal gangue is stored up for a long time, takies a large amount of soils, and polluted water generates H after the spontaneous combustion
2S, SO
3Deng obnoxious flavour, polluted air, and caused the harm of acid rain.The coal gangue of bulk deposition been has also has been occupied increasing arable land, has constituted the dual destruction to ecological and environment.On the other hand, coal gangue is again a kind of natural resources for development and utilization.It is middle micro-that coal gangue (mine and coal separation ore deposit mine tailing) contains more rich organic carbon (organic matter) and the necessary silicon of crop growth, copper, zinc, iron, manganese etc., can improve Soil structure, strengthen the fertility of soil, make increasing crop yield, improve quality of agricultural product.In decades, both at home and abroad obtain many achievements that attract people's attention aspect the improvement of coal gangue and the comprehensive utilization of resources, be mainly used in the following aspects:
(1) uses coal gangue power generation;
(2) make material of construction (colliery wastes brick, cement and mosaic) with coal gangue;
(3) coal gangue is reclaimed and the backfill mine goaf;
(4) reclaim useful composition and produce Chemicals (produce Chemicals such as polymerize aluminum chloride, Tai-Ace S 150, synthesizing series molecular sieve, produce rock wool and goods);
(5) with coal gangue production infant industry filler;
Yet, in these resource conversion, all can cause secondary pollution, supervene some waste gas, sewage, cause serious environmental to pollute.Therefore, along with the enhancing of people's Environmental awareness and going deep into of research degree, pass through modern biotechnology, with coal gangue as matrix and carrier, utilize elite streptomyces microflavus and the colloid bacillus cereus mineralising Decomposition to coal gangue, producing composite microbiological fertilizer will be the optimal path of coal gangue resource conversion.Through the retrieval of patent documentation and related data, do not find close technical scheme.
Summary of the invention: the objective of the invention is for solving the pollution of coal gangue to environment, the natural resources of abundant development and utilization coal gangue, propose a kind of with coal gangue as matrix and carrier, rely on the elite streptomyces microflavus and the effect of colloid bacillus cereus, mineralising is decomposed the multiple element in the coal gangue, for the crop absorption utilization, reach the effect of volume increase and improvement quality.
Main contents of the present invention have:
(1) utilizability of coal gangue as fertilizer sources matrix:
By gathering the coal gangue sample in certain colliery, area, Changzhi, Shanxi, content through Department of Geology of Peking University, national Coal Quality supervision and inspection center, Beijing's its element of new-type fertilizer quality monitoring testing station test analysis, the results are shown in Table 1, by analyzing as can be seen, coal gangue has three effects greatly:
The first, contain the necessary P of plant-growth, K, Ca, Mg, S, Cu, Zn, 9 kinds of elements such as Fe, Mn in the coal gangue, these elements all play the important physical effect to plant.
1. these elements are that plant-growth is necessary, and when this element lacked fully, the growth of plant can not be carried out.
2. after lacking this element, plant can show distinctive illness, and other any chemical elements can not replace its effect, have only this element of replenishing after symptom just can alleviate or disappear.
3. these elements are participated in the metabolism of plant materials directly, and plant is played direct trophism.
The second, coal gangue contains more rich organic carbon (organic matter), after being manured into soil, the organic content in the soil is increased greatly.According to the study, the soil organism is the mineral nutrition of plant and organotrophic source, is again the energy substance of heterotrophic microorganism in the soil, by regulating the growth that C/N can promote the effective microbe in the soil, also is simultaneously the principal element that forms Soil structure.Therefore, the soil organism directly affects the fertilizer tolerability of soil, soil moisture conservation property, and resiliency, tilth, the ventilative situation and the soil moisture etc. are so how much organic content is one of important symbol of soil fertility.In addition, the organic self-cleaning power that also can strengthen soil, the contamination that alleviates objectionable impurities in the soil.Because organic main component is a soil ulmin, and the dissociated functional group of multiple energy is arranged in the soil ulmin, can form the huge legendary turtle compound with multiple pollutent or heavy metal ion, it is water-soluble to strengthen it, make it to shift with water, alleviate its harm, therefore, improving the humus content of the soil is the important measures of pollution abatement harm.This shows that crop applying coal gangue fertilizer not only can not pollute, and also have the pollution of removal, the effect that improves crops quality.
The 3rd, contain a large amount of SiO in the coal gangue
2, its content nearly 50%, it is to exist with silicate and silico-aluminate form, the overwhelming majority is insoluble, and the high resistance weathering; Have only soluble silicon that plant is only effectively; The available silicon of plant mainly is with single silicic acid (H
4SiO
4) form absorbed by crop, it is actually a kind of extremely weak acid.In recent years, the research of emphasis has been carried out in application on plant to element silicon in the world, and find that its effect on plant nutrition is very important, after deliberation, silicon is indispensable nutritive element to crops such as paddy rice, barley, sugarcane and vegetables, has executed after the siliceous fertilizer, obviously promotes the growth of plant, therefore, at present plant nutrition educational circles has and element silicon is decided to be plant nutrition is necessary, the cry of the 17th kind of element.The present invention is by the beneficial microorganism effect, with the SiO of indissoluble
2Be converted into soluble silicon, analyze the rhizosphere soil available silicon content that different tests is handled through Inst. of Plant Nutrition ﹠. Resource, Beijing City Academy of Agricultural ﹠. Fo, by analyzing as can be seen, the green pepper rhizosphere soil available silicon content of amount composite microbiological fertilizer increases by 30.6% than the green pepper rhizosphere soil available silicon content of using aseptic substrate fertilizer in using, the two is except that beneficial microorganism, other compositions are identical, and visible elite streptomyces microflavus and colloid bacillus cereus are to effectively the release action of silicon is quite obvious in the coal gangue.
(2) beneficial microorganism characteristic:
The present invention is through repeatedly screening, composite, and mutually the microorganism of antagonisms is not as the target bacterial classification finally to select two kinds, and selected microbial strains is that the public is available, can buy on the market at home.They are:
1, streptomyces microflavus (Streptomyces microflavus)
(1), thalli morphology:
Sophisticated thalline should be made up of substrate mycelium, aerial mycelium, fibrillae of spores and spore.
The matrix mycelia: claim vegetative mycelium again, elongated, multi-branched, the about 0.5-0.8 μ of diameter m.Do not produce tabula, look shallow is transparent usually; Being interweaved in the growth reticulates structure, can produce the pigment of two kinds of different in kinds, i.e. water colo(u)r and fat-soluble pigment; The former infiltrates in the substratum, makes the corresponding different colours of dying of substratum, as pink, tiger fur is orange-yellow; The latter is colourless in matrix usually, only is dissolved in the organic solvent.
Aerial hyphae: be called for short the gas silk, be after the base silk grows into certain phase, part turn to the space, the aerial mycelium that grows, the gas silk usually than the thick 1-2 of base silk doubly about, color and luster is deeply very easy to be distinguished with the base silk, children age the gas silk mostly be white, after the maturation, especially form fibrillae of spores after, then be annihilated by fibrillae of spores and spore, being difficult to what be differentiated separately has been the gas silk.
Fibrillae of spores: fibrillae of spores can be considered the monogony organ; The vegetative hyphae that it is differentiated by the gas silk with generation spore ability; Fibrillae of spores has certain form, and thin yellow actinomycetes are the relaxed helix shape; Sophisticated fibrillae of spores can be white in color or pink.
Spore: produce through the tabula divisional mode by sophisticated fibrillae of spores, the spore ovalize of streptomyces microflavus, the size of spore is unsettled feature, can not be as the index of diagnostic species.
(2), colonial morphology
On agar plate, 1 spore or mycelia fragment are sprouted and are grown up to the base silk, and form the hypha body with definite shape by a base silk compact winding together and be called bacterium colony; The bacterium colony of streptomyces microflavus is bigger, has an even surface or uneven, and what have has a concentric ring, and what have has a be full of cracks; The bacterium colony surface color depends on the color of spore and spore layer, and the color at the back side depends on the color and the pigment of base silk; The form of bacterium colony and color also change and change to some extent with composition and the culture condition of Pei Ji.
(3), life cycle
Actinomycetic spore is rendered under the environment that is fit to its growth, it is water-swelling, sprout 1-3 germ tube, germ tube grows up to mycelia gradually, forms substrate mycelium, when mycelial growth arrives certain phase, a part grows aerial mycelium to the space, changes into the fibrillae of spores with reproductive performance after the gas silk maturation, and fibrillae of spores produces spore by the tabula divisional mode, spore enters cocycle again, becomes a life cycle.
In the liquid fermenting process, a large amount of fissiparities of mycelia in 20-30 hour form rhizomorph, and mycelia separation, hollow occur, fragments into fragment then, irregular " spore " occur, and shaft-like, ball, ellipse are arranged.
(4), culture condition:
(1) optimum growth temperature is 26 ℃-32 ℃, and the diminuendo of growing more than 36 ℃ below 16 ℃ stops growing more than 50 ℃ below 12 ℃, i.e. death in 5 minutes more than 60 ℃.
(2) at various cake powder, rice bran, crack rice, wheat bran, loam etc. contain well-grown on the organic more substratum, can utilize glucose, sucrose, fructose, starch; Can utilize organonitrogen and inorganic nitrogen, growth needs P, K, Mg, Fe and other trace elements.
(3) PH 6.5-8.5 well-grown, pH value are grown more than 9 below 6 and are suppressed.
(4) require the good aeration condition.
2, colloid bacillus cereus (Bacillus mucilaginosus)
(1), thalli morphology:
Peritrichous, size is 0.9-1.5 * 2-5 μ m, and is shaft-like, the thalline list is given birth to or to life; Gramstaining reaction negative (G
-), it is inhomogeneous to dye; The gemma ellipse, end is given birth to or middle life.Thalline is given birth to the thick-walled pod film.
(2), colonial morphology
On nitrogen-free agar, bacterium colony is circular, projection, and smooth surface, neat in edge, oyster white is thick.
(3), culture condition:
Preferred PH 7.2-7.4, preferred culture temperature is 25-30 ℃.
More than two kinds of bacterial strains be known microorganisms, can both buy on the market, in " bacterial classification secure authentication layered directory " in " fertilizer data registration request " (Ministry of Agriculture announces No. 161) that the Ministry of Agriculture issues, belong to the first step, exempt to do the bacterial classification of secure authentication.And,, show that the selected two kinds of microorganisms of the present invention are to effectively the release processing effect of silicon is quite obvious in the coal gangue by the initial analysis of table 2.
(3) advantage of the present invention:
Compare with other fertilizer such as fertilizer, chemical fertilizer, common compound fertilizer, composite microbiological fertilizer has the following advantages:
1, composite microbiological fertilizer selects for use raw material mostly to be various agriculture and industry wastes, as coal gangue, chicken manure etc., has effectively realized the purpose of solution environmental pollution, " becoming useless (evil) is treasured ".
2, composite microbiological fertilizer contains large amount of organic matter, also contain the necessary nitrogen of crop growth, phosphorus, potassium element and multiple in, trace element, nutrient is comprehensive, elite streptomyces microflavus and colloid bacillus cereus microbial inoculum then as the prerequisite that transforms nutrient, make it to become a kind of complete manure.
3, composite microbiological fertilizer has effectively been realized fertilizer, inorganic fertilizer, microorganism fertilizer three fertilizer are compound, long-acting, quick-acting, the synergy triple effect is compound, for fertile, short living, disease-resistant multi-functional compound.
4, the composite microbiological fertilizer fertilizer efficiency is stablized, is supplied long fertilizers period, after being manured into soil, and the organic nutrient that it is contained, through the beneficial microorganism decomposition and inversion, can discharge various mineral nutrients and be made for absorbing of thing whole growth phase, and constantly discharge carbonic acid gas, promote the photosynthesis of crop.
5, composite microbiological fertilizer contains a large amount of organic matters and soil ulmin, after the beneficial microorganism decomposition and inversion, some soil ulmin has the soil aggregate of promotion and forms, strengthen soil water-retaining, nutrient preserving capability, and can improve the soil arability, improve the soil moisture, thereby improved the function that soil is regulated water, fertilizer, gas, heat, soil fertility also rises thereupon.
6, elite streptomyces microflavus and colloid bacillus cereus flora have improved crop rhizosphere ecological condition on every side in the advantage breeding of crop rhizosphere, the metabolic secretion thing of microorganism can stimulate crop root to grow, raising is to the receptivity of soil nutrient, the antibiont mass-energy of its generation suppresses the infringement of pathogeny bacterium, reaches sick, as the to alleviate disease and pest effect that presses down.
Description of drawings: figure one: microbiobacterial agent technological process of production figure
Wherein: 1 air compressor, 2 compressed air reservoirs, 3 oil water separators, 4 freezing type driers, 5 air total filters, 6 air divide filter 7 colloid bacillus cereus test tubes to cultivate 8 colloid bacillus cereus eggplant-shape bottles to cultivate 9 colloid bacillus cereus shaking tables and cultivate 10 colloid bacillus cereus seed tank culture, 11 colloid bacillus cereus fermentation tank culture, 12 microexamination colloid bacillus cereus qualified 13 and put tank, the peat composed of rotten mosses absorption 14 packing warehouse-in 15 peat composed of rotten mosses steam sterilizings 16 streptomyces microflavus test tubes of weighing and cultivate 17 streptomyces microflavus eggplant-shape bottles and cultivate 18 streptomyces microflavus shaking tables and cultivate the qualified 22 solid medium steam sterilizings of 19 streptomyces microflavus seed tank culture 20 streptomyces microflavus fermentation tank culture, 21 microexamination streptomyces microflavus 23 solid mediums and inoculate 24 solid fermentation casees and cultivate the 25 product dryings 26 packing warehouse-in of weighing
Figure two: composite microbiological fertilizer technological process of production figure
Wherein: high humidity fowl and animal excrements such as 1 chicken manure, pig manure use fowl and animal excrements such as rotary drum drying machine oven dry, sterilization 2 chicken manures, pig manure to use fine pulverizers to pulverize pulverizing 5 elite microbiobacterial agent 6 granulating agents, binding agent 7 various raw materials that 3 coal gangues use Raymond mill to pulverize 4 chemical fertilizer and mix 8 gardens dish tablets press granulation, the 9 oven drying at low temperature machines oven dry 10 packing warehouse-in of weighing
Embodiment: following narration embodiments of the invention:
Embodiment 1: the preparation of streptomyces microflavus microbial inoculum
The method that adopts liquid fermenting to combine with solid fermentation.
1, strain preparation: comprise test tube strains and eggplant-shape bottle bacterial classification, after the aseptic technique inoculation, place 28-32 ℃ constant incubator to cultivate 2-3 days.
Inclined-plane and eggplant-shape bottle culture medium prescription (Gause I substratum):
Saltpetre 1g
Sodium chloride 0.5g
Dipotassium hydrogen phosphate 0.5g
MgSO
4 7H
2O 0.5g
FeSO
4 7H
2O 0.01g
Zulkovsky starch 20g
Agar 20g
Distilled water 1000ml
PH 7.2-7.4
Sterilization pressure 1.1kg/cm
2(sterilizing 30 minutes)
Facing the time spent adds 3% potassium bichromate solution 3.3ml.
2, shaking table is cultivated: temperature 28-32 ℃, the constant temperature shaking table was cultivated 20-24 hour, shaking speed 180r/min.
Shaking table culture medium prescription: (unit: grams per liter)
Sucrose 50
Sal epsom 0.4
Dipotassium hydrogen phosphate 0.4
Potato soaks juice 200
800 milliliters of distilled water
PH 7.2--7.4
Sterilization pressure 1.1kg/cm
2(sterilizing 30 minutes)
3, seed tank culture: adopt 50 liters of mechanical stirring seeding tanks.
Seed tank culture based formulas: (unit: gram)
Sucrose 250
Starch 250
Sodium-chlor 125
Semen Maydis powder 375
25 liters in water
Before PH disappears: after 7.8--8.0 disappears: 7.2--7.5
Culture condition: the seed ullage disappears, and 140 ℃, 1 hour; Substratum is real to disappear, and 121 ℃, half an hour; Be cooled to 30 degree inoculations, inoculum size 1--5%; Culture temperature 28-32 degree; Air flow: 0-10 hour is 1: 0.5, is 1: 1 after 10 hours; Mixing speed 180-200r/min; Tank pressure: 0.5kg/cm
2Fermentation time: 20-24 hour.
4, fermentor cultivation: adopt 500 liters of mechanical stirring fermentor tanks, the same seed tank culture of culture condition and culture medium prescription is cultivated and was put jar in 24 hours.
5, solid culture: fermented liquid is inoculated in the solid medium of the bacterium of going out, inoculum size 5-8% places the solid fermentation case to cultivate culture temperature 28--30 ℃ 5--7 days.Treat that the solid fermentation material all bleaches, water ratio gets final product discharging when 10% left and right sides.
The solid fermentation substratum:
Pond soil 40%
The peat composed of rotten mosses 10%
Lime 2-3%
Water (reach hold agglomerating, that touches promptly looses) 40-50%
PH 7.6-7.8
Embodiment 2: the preparation of colloid bacillus cereus microbial inoculum
Adopt the liquid second order fermentation, fermented liquid adsorbs in 1: 4 ratio with the sterilization peat composed of rotten mosses.
1, strain preparation: comprise test tube strains and eggplant-shape bottle bacterial classification, after the aseptic technique inoculation, place 28-32 ℃ constant incubator to cultivate 2-3 days.
Test tube and eggplant-shape bottle culture medium prescription: (unit: grams per liter)
Sucrose 5.0
Sal epsom 0.5
Lime carbonate 0.1
SODIUM PHOSPHATE, MONOBASIC 2.0
Iron trichloride 0.005
Glass powder 1.0
1000 milliliters of distilled water
Agar 20
PH 7.2--7.4
Sterilization pressure 1.1kg/cm
2(sterilizing 30 minutes)
2, shaking table is cultivated: temperature 28-32 ℃, the constant temperature shaking table was cultivated 20-24 hour, shaking speed 180r/min.
Shaking table culture medium prescription: (unit: grams per liter)
Sal epsom 0.2
Lime carbonate 5.0
Calcium sulfate 0.1
Dipotassium hydrogen phosphate 0.2
Sodium-chlor 0.2
Ammonium sulfate 0.2
1000 milliliters of distilled water
PH 7.2--7.4
Sterilization pressure 1.1kg/cm
2(sterilizing 30 minutes)
3, seed tank culture: adopt 50 liters of mechanical stirring seeding tanks.
Seed tank culture based formulas: (unit: gram)
Sucrose 25
Starch 200
Sal epsom 12.5
Lime carbonate 2.5
Potassium primary phosphate 100
Iron trichloride 0.25
Ammonium sulfate 25
25 liters in water
PH 7.2-7.4
Culture condition: the seed ullage disappears, and 140 ℃, 1 hour; Substratum is real to disappear 121 ℃, half an hour; Cooling 30 degree inoculations, inoculum size 5-10%; Culture temperature 28-32 ℃; Air flow: 0-10 hour is 1: 0.5, is 1: 1 after 10 hours; Mixing speed 270-300r/min; Tank pressure: 0.5kg/cm
2Fermentation time: 20-24 hour.
4, fermentor cultivation: adopt 500 liters of mechanical stirring fermentor tanks, culture condition is identical with seed tank culture with culture medium prescription, incubation time 24-28 hour, can put a jar absorption when thalline forms gemma more than 90% in the fermented liquid.
Embodiment 3: the preparation of composite microbiological fertilizer
The present invention also can use multiple organic wastes such as fowl and animal excrement, crop stalk, food-processing industry tankage except the waste-coal gangue of a large amount of uses colliery.
1, raw material is handled
1. high humidity organic wastes such as chicken manure, straw are sent into the cylinder dryer, under 650-700 ℃ hot environment, rapid drying moisture, thoroughly kill pathogenic bacteria reaches innoxious.
2. the organic waste after will drying is sent into ultrafine crusher and is crushed to the 80-100 order.
3. use Raymond mill that colliery powder is broken to 80 orders.
2, mix
According to fertilizer formula, the coal gangue that crushes, organic waste, chemical fertilizer, microbiobacterial agent etc. are dropped into duplex bevel-type mixer according to proportioning mix.
3, granulation: the material that mixes is sent into garden dish tablets press granulation.
4, cryodrying: fertiliser granulates is sent into the oven drying at low temperature machine, and the product water ratio is about 10%.
5, packing warehouse-in: 50 kilograms every bag, guard against damp anti-high temperature during storage
6, composite microbiological fertilizer prescription (is example with total nutrient content 15%):
Coal gangue 40%
Urea 11%
Cicken feces dried 20%
Microbiobacterial agent (elite streptomyces microflavus and colloid bacillus cereus) 5%
7, target level of product quality and technical indicator
A. total nutrient (N+P
2O
5+ K
2O) 〉=15%;
B. organic content 〉=25%;
C. living bacteria count 〉=0.2 hundred million/gram
D moisture content≤15%
E particle 2.5--4.5 millimeter
F. (As, Hg, Pb, Cr, Cd) content is all less than relative national standards (NY 227--94)
Embodiment 4: to the production-increasing function of green pepper
1, for studying the article kind: the eggplant door
2, for examination soil: soil type is a Cinnamon Soils, and quality is middle earth, and soil nutrient status is: organic 27.167g/kg, full nitrogen 0.564g/kg, available potassium 276mg/kg, available phosphorus 9.2mg/kg.
3, for the examination place: Changzhi City Huguan County, Shanxi Province seed multiplication farm
4, test Treatment Design: establish 7 processing altogether
1. ck (blank);
2. amount composite microbiological fertilizer (total nutrient content is 25%) in, mu is executed 100 kilograms and is made base fertilizer;
3. aseptic substrate contrast, mu execute 100 kilograms of composite microbiological fertilizers after the deactivation do base fertilizer (aseptic substrate fertilizer with middle amount bio-fertilizer after cobalt-60 inactivation treatment and obtain.Except that not containing the beneficial microorganism, the two is identical for other compositions.)；
4. common fertilizer of equal value is pressed habits fertilization, and mu is granted the chemical fertilizer of price such as composite microbiological fertilizer and made base fertilizer;
5. wait the nutrient chemical fertilizer, according to the nutrients ratio and the content of middle amount composite microbiological fertilizer, the chemical fertilizer that equates that mu is granted is made base fertilizer;
6. low amount composite microbiological fertilizer, mu is executed 80 kilograms and is made base fertilizer;
7. a large amount composite microbiological fertilizer, mu is executed 120 kg and is made base fertilizer.
5, sub-district, field design:
In strict accordance with the national Ministry of Agriculture " registration fertilizer efficiency experimental technique rules (provisional) " design, 7 meters of zone leaders, wide 3 meters, 21 meters of net areas
2, south-north direction is established wide 2 chis in aisle between district's group, stay 30 centimetres on the furrow ridge, and 1 meter of protection line width is taked to distinguish at random group and is arranged, 3 repetitions.
6, yield result analysis:
Result from table 3, what mean yield was the highest is to handle 2 (middle amount composite microbiological fertilizers), it is 4364.09 kilograms/mu, stimulation ratio than blank is 49.6%, stimulation ratio than the aseptic substrate contrast is 9.1%, the amount composite microbiological fertilizer has good effect of increasing production to green pepper in the explanation, and beneficial microorganism also can improve crop yield (comparing conclusion with aseptic substrate).
By table 4 L.S.D method The result of multiple comparisons, show:
1. each is handled than handling 1 (blank) output and has extremely significant fertilizer efficiency difference;
2. handle 2,5,6,7 than handling the extremely significant fertilizer efficiency difference of 3 (aseptic substrate contrast) output existence;
3. handle 2,7 than handling 4 there is extremely significant fertilizer efficiency difference in (equivalence format fertilizer) output;
4. volume variance is not remarkable between handling 2,5,6,7.
Table 1: various constituent content analysis tables in the coal gangue
| Sequence number | Interventions Requested | Unit | Measured |
| 1 | SiO 2 | ％ | 48.57 |
| 2 | Fe 2O 3 | ％ | 1.77 |
| 3 | CaO | ％ | 0.81 |
| 4 | MgO | ％ | 0.54 |
| 5 | K 2O | ％ | 1.31 |
| 6 | P 2O 5 | ％ | 0.20 |
| 7 | Full sulphur | ％ | 0.02 |
| 8 | MnO | ％ | 0.04 |
| 9 | Cu | ppm | 35 |
| 10 | Zn | ppm | 55 |
| 11 | Total amount of organic | ％ | 14.1 |
Table 2: different fertilizer is handled the influence to available silicon content in the green pepper rhizosphere soil (coal gangue)
| The check numbering | The sample title | Effective silicon (Si) mg/ |
| 1 | Green pepper rhizosphere soil (amount bio-fertilizer in executing) | 299 |
| 2 | Green pepper rhizosphere soil nutrient chemical fertilizer such as () executing | 232 |
| 3 | Green pepper rhizosphere soil (executing aseptic substrate fertilizer) | 229 |
Table 3: green pepper test yield result table
Unit: kilogram/mu
The output L.S.D method comparative analysis of table 4 green pepper experimental plot
Unit: kilogram
| Handle title | Mean yield (X) (having big) to little | X-91.8 | X-125.9 | X-130.2 | X-136.2 | X-136.5 | X-137.0 |
| Measure composite microbiological fertilizer in 2 | 137.4 | 45.6 ** | 11.5 ** | 7.2 ** | 1.2 | 0.9 | 0.4 |
| 7 a large amount composite microbiological fertilizers | 137.0 | 45.2 ** | 11.1 ** | 6.8 ** | 0.8 | 0.5 | |
| 6 low amount composite microbiological fertilizers | 136.5 | 44.7 ** | 10.6 ** | 6.3 * | 0.3 | ||
| 5 chemical fertilizer such as nutrient such as grade | 136.2 | 44.2 ** | 10.3 ** | 6 * | |||
| The 4 equivalences fertilizer of formatting | 130.2 | 38.4 ** | 4.1 | ||||
| 3 aseptic substrates (contrast) | 125.9 | 34.1 ** | |||||
| 1CK (blank) | 91.8 | ||||||
| Annotate: 5%L.S.D=T 0.05XS X1-X2＝4.71 1％L.S.D＝T 0.01XS X1-X2＝6.61 S X1-X2On behalf of significant difference * * ,=2.16 * represent utmost point significant difference | |||||||
Claims (4)
1, a kind of is the composite microbiological fertilizer of matrix with the coal gangue, it is characterized in that: it is as matrix and carrier with colliery waste-coal gangue, organic waste and an amount of chemical fertilizer such as compound chicken manure, pig manure through harmless treatment, rely on the elite streptomyces microflavus and the effect of colloid bacillus cereus, mineralising is decomposed the necessary P of plant-growth in the coal gangue, K, Ca, Mg, S, Cu, Zn, 9 kinds of elements such as Fe, Mn and organic and a large amount of SiO
2, with the SiO of indissoluble
2Be converted into soluble silicon,, reach the effect of volume increase and improvement quality for the crop absorption utilization.
2, described according to claim 1 is the composite microbiological fertilizer of matrix with the coal gangue, and it is characterized in that: said elite streptomyces microflavus and colloid bacillus cereus are followed successively by:
1. streptomyces microflavus (Streptomyces microflavus)
2. colloid bacillus cereus (Bacillus mucilaginosus)
3, according to claim 1 or 2 described be the composite microbiological fertilizer of matrix with the coal gangue, it is characterized in that: the green pepper rhizosphere soil available silicon content of amount composite microbiological fertilizer increases by 30.6% than the green pepper rhizosphere soil available silicon content of using aseptic substrate fertilizer in using, the two is except that beneficial microorganism, other compositions are identical, and visible elite streptomyces microflavus and colloid bacillus cereus are to effectively the decomposition release action of silicon is quite obvious in the coal gangue.
4, according to claim 1 or 2 described be the composite microbiological fertilizer of matrix with the coal gangue, it is characterized in that: said composite microbiological fertilizer is by elite streptomyces microflavus, colloid bacillus cereus, coal gangue, fowl and animal excrement such as chicken manure, pig manure, chemical fertilizer such as nitrogen, phosphorus, potassium, binding agent is formed, and wherein the weight percent of each material is: (is example with total nutrient content 15%):
Coal gangue 40%
Urea 11%
Coarse whiting 9%
Repone K 10%
Cicken feces dried 20%
Microbiobacterial agent (elite streptomyces microflavus and colloid bacillus cereus) 5%
Binding agent 5%
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100568694A CN1740122A (en) | 2004-08-27 | 2004-08-27 | Composite microbial fertilizer with coal gangue as matrix and its production process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100568694A CN1740122A (en) | 2004-08-27 | 2004-08-27 | Composite microbial fertilizer with coal gangue as matrix and its production process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1740122A true CN1740122A (en) | 2006-03-01 |
Family
ID=36092677
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2004100568694A Pending CN1740122A (en) | 2004-08-27 | 2004-08-27 | Composite microbial fertilizer with coal gangue as matrix and its production process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1740122A (en) |
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100569904C (en) * | 2007-04-30 | 2009-12-16 | 西北农林科技大学 | Storage-expanding compatibilizer prepared from coal gangue and production method thereof |
| CN101913950A (en) * | 2010-08-19 | 2010-12-15 | 范鸿基 | Organic fertilizer preparation method and multi-strain breeding of biodegradable coal gangue |
| CN102173917A (en) * | 2011-01-06 | 2011-09-07 | 四川大学 | Preparation method of afforestation matrices for sandstone rock slopes |
| CN102173919A (en) * | 2011-01-07 | 2011-09-07 | 四川大学 | Preparation method of afforestation matrices for mudstone rock slopes |
| CN103319248A (en) * | 2013-06-27 | 2013-09-25 | 苏州禾润农业科技有限公司 | Microorganism fertilizer for preventing and treating valsa mali of lettuce and preparation method thereof |
| CN103641605A (en) * | 2013-11-26 | 2014-03-19 | 大连民族学院 | High-fertility long-acting anti-verticillium-wilt biological bacterial fertilizer and preparation method thereof |
| CN104788265A (en) * | 2015-04-30 | 2015-07-22 | 山西省农业科学院农业环境与资源研究所 | Saline-alkali soil biological improved fertilizer based on high-sulfur gangue and preparation method thereof |
| CN105018383A (en) * | 2015-07-27 | 2015-11-04 | 江西省林业科学院 | Microbial compound bacterium agent and method for improving coal gangue wasting land afforestation survival rate |
| CN107142230A (en) * | 2017-05-16 | 2017-09-08 | 贵州大学 | A kind of M. luteus bacterial strain and application thereof |
| CN107236683A (en) * | 2017-05-16 | 2017-10-10 | 贵州大学 | A kind of slow aureus strain and application thereof |
| CN107602243A (en) * | 2017-10-17 | 2018-01-19 | 广西平乐农药厂 | A kind of microbial manure and preparation method thereof |
| CN108794108A (en) * | 2018-02-06 | 2018-11-13 | 安徽新安古建园林建设股份有限公司 | A kind of afforestation organic fertilizer and preparation method thereof |
| CN109896840A (en) * | 2019-04-26 | 2019-06-18 | 珠海三豫环保新材料科技有限公司 | A method of porous biological carrier is prepared using gangue |
| CN109928782A (en) * | 2019-04-26 | 2019-06-25 | 珠海三豫环保新材料科技有限公司 | A kind of method that gangue prepares siliceous fertilizer |
| CN109942347A (en) * | 2019-01-31 | 2019-06-28 | 贵州大学 | A method of making microbial manure using coal gangue as raw material |
| WO2019127119A1 (en) * | 2017-12-27 | 2019-07-04 | 深圳市大富科技股份有限公司 | Soilless planting method for graphite-based organic fertilizer slurry |
| CN110999753A (en) * | 2019-12-26 | 2020-04-14 | 北京林业大学 | Coal gangue-based artificial ecological matrix and preparation method thereof |
| CN111792952A (en) * | 2020-07-09 | 2020-10-20 | 北京锦绣新技术发展有限公司 | Active coal gangue micro powder, active coal gangue mineral fertilizer and preparation method |
| CN112056177A (en) * | 2020-09-17 | 2020-12-11 | 曲靖市麒麟区绿宝肥料制造有限公司 | Method for preparing rice seedling raising matrix by using solid waste |
-
2004
- 2004-08-27 CN CNA2004100568694A patent/CN1740122A/en active Pending
Cited By (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100569904C (en) * | 2007-04-30 | 2009-12-16 | 西北农林科技大学 | Storage-expanding compatibilizer prepared from coal gangue and production method thereof |
| CN101913950A (en) * | 2010-08-19 | 2010-12-15 | 范鸿基 | Organic fertilizer preparation method and multi-strain breeding of biodegradable coal gangue |
| CN102173917A (en) * | 2011-01-06 | 2011-09-07 | 四川大学 | Preparation method of afforestation matrices for sandstone rock slopes |
| CN102173919A (en) * | 2011-01-07 | 2011-09-07 | 四川大学 | Preparation method of afforestation matrices for mudstone rock slopes |
| CN102173919B (en) * | 2011-01-07 | 2013-01-30 | 四川大学 | Preparation method of afforestation matrices for mudstone rock slopes |
| CN103319248B (en) * | 2013-06-27 | 2015-02-11 | 苏州禾润农业科技有限公司 | Microorganism fertilizer for preventing and treating valsa mali of lettuce and preparation method thereof |
| CN103319248A (en) * | 2013-06-27 | 2013-09-25 | 苏州禾润农业科技有限公司 | Microorganism fertilizer for preventing and treating valsa mali of lettuce and preparation method thereof |
| CN103641605A (en) * | 2013-11-26 | 2014-03-19 | 大连民族学院 | High-fertility long-acting anti-verticillium-wilt biological bacterial fertilizer and preparation method thereof |
| CN103641605B (en) * | 2013-11-26 | 2015-02-11 | 大连民族学院 | High-fertility long-acting anti-verticillium-wilt biological bacterial fertilizer and preparation method thereof |
| CN104788265A (en) * | 2015-04-30 | 2015-07-22 | 山西省农业科学院农业环境与资源研究所 | Saline-alkali soil biological improved fertilizer based on high-sulfur gangue and preparation method thereof |
| CN104788265B (en) * | 2015-04-30 | 2017-07-14 | 山西省农业科学院农业环境与资源研究所 | Salt-soda soil biological modification fertilizer and its preparation based on high sulfur coal gangue |
| CN105018383A (en) * | 2015-07-27 | 2015-11-04 | 江西省林业科学院 | Microbial compound bacterium agent and method for improving coal gangue wasting land afforestation survival rate |
| CN105018383B (en) * | 2015-07-27 | 2018-10-02 | 江西省林业科学院 | A kind of complex microbial inoculum and method improving the Coal Wasting Lands surrival rate of afforestation |
| CN107236683B (en) * | 2017-05-16 | 2020-05-26 | 贵州大学 | Slow staphylococcus strain and application thereof |
| CN107142230A (en) * | 2017-05-16 | 2017-09-08 | 贵州大学 | A kind of M. luteus bacterial strain and application thereof |
| CN107236683A (en) * | 2017-05-16 | 2017-10-10 | 贵州大学 | A kind of slow aureus strain and application thereof |
| CN107142230B (en) * | 2017-05-16 | 2020-05-26 | 贵州大学 | Garcinia microsphere strain and application thereof |
| CN107602243A (en) * | 2017-10-17 | 2018-01-19 | 广西平乐农药厂 | A kind of microbial manure and preparation method thereof |
| WO2019127119A1 (en) * | 2017-12-27 | 2019-07-04 | 深圳市大富科技股份有限公司 | Soilless planting method for graphite-based organic fertilizer slurry |
| CN108794108A (en) * | 2018-02-06 | 2018-11-13 | 安徽新安古建园林建设股份有限公司 | A kind of afforestation organic fertilizer and preparation method thereof |
| CN109942347A (en) * | 2019-01-31 | 2019-06-28 | 贵州大学 | A method of making microbial manure using coal gangue as raw material |
| CN109942347B (en) * | 2019-01-31 | 2021-06-15 | 贵州大学 | Method for preparing microbial fertilizer by taking coal gangue as raw material |
| CN109928782A (en) * | 2019-04-26 | 2019-06-25 | 珠海三豫环保新材料科技有限公司 | A kind of method that gangue prepares siliceous fertilizer |
| CN109896840A (en) * | 2019-04-26 | 2019-06-18 | 珠海三豫环保新材料科技有限公司 | A method of porous biological carrier is prepared using gangue |
| CN110999753A (en) * | 2019-12-26 | 2020-04-14 | 北京林业大学 | Coal gangue-based artificial ecological matrix and preparation method thereof |
| CN110999753B (en) * | 2019-12-26 | 2021-07-27 | 北京林业大学 | Coal gangue-based artificial ecological matrix and preparation method thereof |
| CN111792952A (en) * | 2020-07-09 | 2020-10-20 | 北京锦绣新技术发展有限公司 | Active coal gangue micro powder, active coal gangue mineral fertilizer and preparation method |
| CN112056177A (en) * | 2020-09-17 | 2020-12-11 | 曲靖市麒麟区绿宝肥料制造有限公司 | Method for preparing rice seedling raising matrix by using solid waste |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1054362C (en) | Method for producing organic and inorganic mixed microbe fertilizer | |
| CN101037370A (en) | Whole-fertilizer compound fertilizer and preparation method | |
| CN1030761C (en) | Compound organic fertilizer containing multiple bacterial spawns and preparation thereof | |
| CN1740122A (en) | Composite microbial fertilizer with coal gangue as matrix and its production process | |
| CN1304912A (en) | Process for preparing microecological organic fertilizer | |
| CN110184220B (en) | Efficient phosphate and potassium solubilizing bacterium and application thereof | |
| CN102212496B (en) | Bacillus mucilaginosus and applications thereof | |
| CN1974492A (en) | Process of twice fermenting garbage-sludge mixture to produce microbial fertilizer | |
| CN1618772A (en) | High efficiency biological organic fertilizer and its production technology | |
| CN102336614A (en) | Nutrient matrix for improving saline-alkali soil and preparation method thereof | |
| CN102199051A (en) | Trichoderma microbial fertilizer production by reutilizing livestock and poultry manure | |
| CN113321547A (en) | Rhizosphere growth promoting water-soluble microbial fertilizer and preparation method thereof | |
| CN1165127A (en) | Method for producing mixed culture biological organic inorganic compound granulated fertilizer | |
| CN101696391B (en) | Rapid composting microbial inoculum of agricultural wastes and method for preparing organic fertilizer from the same | |
| WO2023124450A1 (en) | Bacillus velezensis strain and use thereof | |
| CN1163446C (en) | Microbial streptomycete fertilizer and production process | |
| CN104926535A (en) | Spirulina peptide bio-organic fertilizer | |
| CN113773986B (en) | Microbial inoculum for rapid temperature rise of organic solid waste aerobic fermentation in winter and preparation method thereof | |
| CN1056959C (en) | Efficient peanut yield increasing agent and its preparation | |
| CN102432366A (en) | Method for producing organic-inorganic compound fertilizer with silkworm excrement as organic raw material | |
| CN104909846A (en) | Bacillus amyloliquefaciens HFJ-7-containing composite chicken manure decomposed starter and application thereof | |
| CN1286776C (en) | Composite microbial manure and method for production thereof | |
| CN103772008B (en) | A kind of composite microbiological fertilizer and production method thereof with efficient disease-proof functions | |
| Gopalakrishnan et al. | Composting of sweet sorghum bagasse and its impact on plant growth promotion | |
| CN1445200A (en) | Method for producing fertilizer by using acid or alkali materials to process vegetal materials |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20060224 Address after: 046204 Hou Town, Xiangyuan County, Changzhi, Shanxi Applicant after: Shanxi Lu'an Mining Industry (Group) Co., Ltd. Co-applicant after: LU'AN VOCATIONAL & TECHNICAL COLLEGE Address before: Beijing city Tongzhou District five Shop No. 6 West xinhualianjinyuan 3-741 Applicant before: Ruan Dong |
|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |