CN1165127A - Method for producing mixed culture biological organic inorganic compound granulated fertilizer - Google Patents
Method for producing mixed culture biological organic inorganic compound granulated fertilizer Download PDFInfo
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Abstract
A method for producing granular built-up fertilizer includes such steps as preparing multi-bacterial biofertilizer and building-up with organic, inorganic and trace elements, all of which are synergistic with each other to fully use biological bacterial group and accelerate the decomposition of inorganic P and K, benefiting crops to suck and increasing Yield. Its advantages include simple production process and low cost. It is suitable as base fertilizer.
Description
The present invention relates to a kind of production method of many bacterium biological organic inorganic compound fertilizer, belong to a kind of many bacterium of use biology, inorganic, organic compound integrated approach, in organic and inorganic fertilizer material, add many bacterium bio-feritlizer, the short long agent of preferred optimum formula and interpolation farm crop finally produces the biological compounded granular fertilizer of efficient many bacterium.
Have many pieces of patent documents to disclose all methods of utilizing biological bacteria to produce compound manure in the prior art, wherein immediate documents is:
921000270 application number Chinese patents disclose the method for utilizing the biological bacteria organism to produce many bacterium biological organic complex fertilizer, it utilizes organism chicken manure, soya-bean cake and inorganics ground phosphate rock, zinc sulfate and biological bacteria thereof to mix production composite fertilizer, and its shortcoming is: the one, and biological bacteria adopts liquid, and the enlarged culturing facility investment is big step by step; Two is that enlarged culturing is not easy to operate simultaneously for 5 bacterial classifications; The 3rd, the ratio that mineral substance mineral manure adds is few, and fertilizer efficiency is low.
951148664 application number Chinese patents disclose the method for utilizing biologic inorganic, organic raw material to produce composite bio-fertilizer, it utilizes biological bacteria and organic and inorganic raw material mixing production composite fertilizer, its shortcoming is: the one, and grain and by product ratio that the bacterial manure fermentation culture adopts are big, the cost height; The 2nd, it is too high to mix back granulation bake out temperature, easily active bacterium is killed.
The 95113213X Chinese patent discloses the compound manure that utilizes humic acids and preparation of raw material such as nitrogen, phosphorus, potassium and bio-bacterial manure trace element to form, and its shortcoming is: the one, and organic matter raw material cooperates ratio few, has increased the investment of raw material; The 2nd, mentioned the adding biological bacteria, do not propose but the title of biological bacteria and preparation method are concrete, not exclusively open, be difficult to implement to produce.
China is a large agricultural country, land resources is wide, agricultural byproducts tankage agricultural crop straw and waste resource thereof are abundant especially, these raw materials are most of burned or discarded, severe contamination environment and wasted effective resource, utilize agricultural byproducts tankage and domestic animal thereof to hold ight soil and handle through biological fermentation and be mixed with multi-component composite fertilizer, can save the mineral manure raw material, improve environmental pollution, improve the vigor of soil;
The production method that the objective of the invention is to overcome the prior art deficiency and a kind of many bacterium biological organic inorganic compound fertilizer is provided, this method is used farm crop by product and domestic animal thereof to hold ight soil to be the bio-bacterial manure of many bacterium of medium preparation, with the treated and inorganics of organic waste and act synergistically polynary the cooperation mutually of plant growth accelerator, it is good to make it comprehensive fertilizer efficiency; It is big to adopt low temperature to reach the organic matter raw material ratio below 50 ℃ in granulation and drying course thereof, and bio-bacterial manure cooperates with organism earlier when mixing granulation, has effectively protected the activity of many bacterium bio-bacterial manure.
Method for producing mixed culture biological organic inorganic compound granulated fertilizer of the present invention comprises following step:
1) preparation of raw material comprises the preparation of bio-bacterial manure raw material and the preparation of bacterial classification, separation, purifying, mutagenesis;
2) prescription of organic matter raw material and preparation;
3) prescription of inorganics raw material and preparation;
4) prescription of many bacterium biologic organic, inorganic composite fertilizer and preparation;
Wherein the prescription of bio-bacterial manure raw material is:
Agricultural crop straw powder (15-20 order) 20%, domestic animal is held ight soil loam 60-70%, cottonseed cake or wheat bran 20-30%, calcium superphosphate 1-1.5%, plant ash or Coal Ash 5-10%, regulating the pH value with lime is about 7.5, the water addition ratio example is advisable 1.5kg/cm to hold agglomerating not dripping
2Pressure sterilization 30 minutes or normal-pressure sterilization 2 hours cool to and get final product inoculation fermentation or the unsterilised fermentation of increasing inoculum size below 35 ℃;
The solid spawn of inserting in the culture material is:
Bacillus megaterium ACCC10010 and bacillusmusilaginosiengineering ACCC10012 inoculum size 3-5%; Or
Bacillus megaterium ACCC10011 and bacillusmusilaginosiengineering ACCC10012 and Jingyang streptomycete ACCC40021 inoculum size 6-8%; Or
Blown-ball Azotobacter ACCC10001 and bacillus megaterium ACCC10010 and the unsterilised fermentation of bacillusmusilaginosiengineering ACCC10012 inoculum size 13-20% culture material.
The prescription of organic matter raw material and being prepared as:
1. agricultural crop straw (15-20 order) 25-30% 2. domestic animal hold poultry manure 55-60%
3. active pond sludge 15-20% is plant ash or cinder ash 5-10% 4.
5. lime 1-2%
Above material thorough mixing is even, and humidity is advisable to hold agglomerating not dripping, and piles up sealing spontaneous fermentation more than 96 hours, spreads out to dry or dry and pulverizes the 15-20 order and store standby.
The prescription of inorganics raw material and being prepared as:
1. 2. fused(calcium magnesium)phosphate 15-18% of urea 34-36%
3. 4. Repone K 30-35% of calcium superphosphate 15-18%
5. 6. lime carbonate 2-4% of sal epsom 2-4%
7. 8. zinc sulfate 0.2-0.3% of boric acid 0.1-0.2%
9. 10. ammonium molybdate 0.01-0.03% of manganous sulfate 0.3-0.4%
copper sulfate 1-1.2% Xanthohumate 0.1-0.2%
rare earth 0.08-0.1%
Above raw material thorough mixing was pulverized the 15-20 order, packed standby;
The prescription of compound manure and preparation:
1. bacillus megaterium ACCC10010 and bacillusmusilaginosiengineering ACCC10012; Or
Bacillus megaterium ACCC10011 and bacillusmusilaginosiengineering ACCC10013 and Jingyang streptomycete ACCC40021 thereof; Or
Blown-ball Azotobacter ACCC10001 and bacillus megaterium ACCC10010 and bacillusmusilaginosiengineering ACCC10012 compound bacterial fertilizer 10-15% thereof.
2. mineral manure 40-45%
3. organic corruption thing and fertilizer 40-45%
The preparation mixing granulation:
Earlier compound bacterial fertilizer is inserted in the mixer and mix with organic fertilizer; again inorganic material is added in the mixer and mix; the compound manure that mixes is arrived the tablets press granulation by transfer roller, and oven drying at low temperature promptly becomes mixed culture biological organic inorganic compound granulated fertilizer.
In the present invention, many bacterium biological flora and employing agricultural byproducts tankage and domestic animal are held the substratum of ight soil as biological flora, and add of the short long agent of a spot of phosphate fertilizer as biology, preferably resolve the growth that to satisfy biological flora, also processing environment pollution problem preferably makes biological flora play synergy in compound manure.Wherein, the bacillus megaterium phosphorus bacteria bacterium that can resolve into available phosphorus to the organophosphorus of invalid attitude in the soil or inorganic phosphorus; Bacillusmusilaginosiengineering potassium bacterial fertilizer, it can decompose the potassium in the mineral out, can obtain the plain nutrition of phosphorus again from hard phosphorus ash, can also be from air fixing nitrogen, can strengthen the crop disease-resistant ability simultaneously; Blown-ball Azotobacter can be can not being changed into by the absorbent nitrogen nutriment of farm crop by 80% the nitrogen that farm crop utilize in the air; The Jingyang streptomycete can produce and irritate element and antibiotic, farm crop is had diseases prevention, keeps a full stand of seedings, loosens the soil and stimulate multiple effects such as plant growth; Mineral manure has quick-acting to farm crop, is the main raw material of this composite fertilizer, and they participate in the vital movement of crop in every way, plays a part in the vegetable cell active compound on the constituent and metabolism; Trace element has single-minded physiology function in plant-growth, they interdepend in the whole growth process of plant, condition each other, and are in a kind of equilibrium state; Fertilizer can improve the effect of fertilizer, reduces cost, and can be the carrier that the existence of biological bacteria in compound manure is played protection and nutrition is provided;
The production method of many bacterium biological organic inorganic compound fertilizer of the present invention has the following advantages and positively effect:
1, wastes of crops and domestic animal to hold the ight soil consumption many, improved fertilizer efficiency, reduced cost, and the condition of existence be provided for biological bacteria;
2, adopt many bacterial strains compound bacterial fertilizer, improved the comprehensive effectiveness of biological bacteria greatly, played collaborative mutually effect the inorganic fertilizer accelerate decomposition;
3, adopt many bacterium biology, organic and inorganic, trace element and the compound preparation of plant growth agent, improved fertilizer efficiency greatly and reduced cost, have the function of full-elementary fertilizer, be suitable for all kinds of farm crop and use;
4, be rich in bio-active substance and somatomedin, a large amount of biological bacteria apply can be a large amount of behind the land for growing field crops breeding, have stimulate increase, the effect of diseases prevention;
5, prior biological composite fertilizer to use scope little, the present invention goes for using of all kinds of farm crop, is processed into granulated fertilizer, has improved the long-lasting of fertilizer greatly and has reduced the loss of effective ingredient, is easy to store and transportation;
6, the present invention is owing to adopt many bacterium biological flora and organism, and the decomposition and the utilization that can improve mineral manure greatly improve hardening of soil property and soil, help the benign cycle in soil;
7, adopt agricultural byproducts tankage and domestic animal to hold ight soil and cooperate compositely, refuse is fully used, improved ecotope, its economic benefit and social benefit are quite remarkable.
For introducing advantage of the present invention and effect in detail, quote some charts below and be elaborated:
Accompanying drawing is technical process of the present invention
The product effective constituent table of table 1 for utilizing method of the present invention to make;
The test of products situation of table 2 for utilizing method of the present invention to make.
The present invention utilizes microorganism bacillus megaterium, bacillusmusilaginosiengineering, blown-ball Azotobacter, Jingyang streptomycete and organism domestic animal to hold ight soil, inorganics azophoska, trace element and plant growing cooperation thereof and produces mixed culture biological organic inorganic compound granulated fertilizer.
The present invention can utilize part farm crop by product to make many bacterium bio-feritlizer by the physical treatment biological fermentation, adds then that organic fertilizer and agricultural crop straw domestic animal thereof are held ight soil (through the sealing fermentative processing), add mineral manure (nitrogen, phosphorus, potassium), trace element, plant growth accelerator fit in mixed culture biological organic inorganic compound granulated fertilizer.Technology of the present invention is simple, for many bacterium biological culture material, can carry out unsterilised solid fermentation, the solid fermentation of also can sterilizing, for the former, saved the investment of sterilizing device, but inoculum size will be greatly a bit for the latter, because of need sterilizing device and boiler, invest greatlyyer, but inoculum size is little;
Adopt many bacterium biological fermentation bacterial manure, utilizing agricultural crop straw and domestic animal to hold ight soil handles through the sealing fermentating drying, fit in all element compound fertilizer with inorganic, trace element, be applicable to the fertilizer of all kinds of farm crop and cash crop thereof, its economic benefit and social benefit are quite remarkable.
The composite fertilizer that utilizes bio-bacterial manure of many bacterium and organic-inorganic and trace element thereof to be mixed with has all obtained good effect through examining then to reach to use, and every technical indicator meets national fertilizer standard, through land for growing field crops fertilizer efficiency lab scale, has all obtained good effect.Present technique from many bacterial classifications processing fermentating drying, inorganics, the trace element of making, organism agricultural crop straw and ight soil thereof fit in the biological compounded granular fertilizers of many bacterium, compare with complete first mineral manure, price has reduced more than 15%, and fertilizer efficiency improves about 10%.
The granulated fertilizer that present technique is made is through field experiment, has the mineral manure of saving, stimulates farm crop to increase, improve fertilizer utilization ratio, reduces fertilizer cost, advantage such as improve the ecological environment.
Used following bio-bacterial manure and inorganic fertilizer, fertilizer, trace element among the present invention, wherein biological inoculum needs to buy mutagenesis separation of produced bacterial classification from Chinese microorganism strain agricultural microorganism center; Mineral manure nitrogen, phosphorus, potassium are bought from market; Organism and fertilizer thereof are collected fermentating drying by relevant department and are handled; The short long agent of trace element and farm crop thereof is bought by market.The bio-bacterial manure of many bacterium has the effect of decomposing inorganic organonitrogen, phosphorus, potassium; Organic fertilizer the biological bacteria of providing existence is provided and reduces the effect of composite fertilizer's cost; Mineral manure is the main raw material of present technique composite fertilizer; The short long agent of trace element and farm crop thereof is the effect of physiology of balance.
Divide seven steps to introduce the preparation of former bacterial classification respectively below; The separation of bacterial classification and purifying mutagenesis; The preparation of bio-bacterial manure; Bacterial manure plate live bacterial count; The cooperation of inorganic raw material and trace element; Organic cooperation and processing; The prescription of composite fertilizer and making.
(1) making of bacterial classification
1) bacillus megaterium ACCC10010 bacterial classification is produced
1. slant strains substratum:
Albumen freezes 0.4-0.6%, NaCl 0.4-0.6%, extractum carnis 0.2-0.4%, agar 1.3-1.6%, distilled water 100ml, pH value nature.
2. the solid spawn substratum is:
Rice bran (wheat bran or cottonseed cake) 25-35%, straw powder 10-15%, domestic animal is held ight soil and coal ash dregs 60-70%, and calcium superphosphate 1-1.5% uses the liming adjust pH, adds water and is advisable to hold agglomerating not dripping.
2) bacillusmusilaginosiengineering ACCC10012 bacterial classification is produced
1. slant strains substratum:
Liver immersion liquid 100ml, NaCl 0.4-0.6%, peptone 0.8-1.2%, agar 1.8-2.3%, pH value 7.
2. the solid spawn substratum is:
Wheat bran or cottonseed cake 25-35%, straw powder 15-20%, domestic animal is held ight soil and loam 55-65%, calcium superphosphate 1-1.5%, pH value 7 adds water and is advisable to hold agglomerating not dripping.
3) bacillus megaterium ACCC10011 bacterial classification is produced
1. the slant strains substratum is:
Potato vat liquor 50ml, sucrose 1.5-2.5%, agar 1.5-2.5%, distilled water 50ml, pH value nature.
2. the solid spawn substratum is:
Rice bran (wheat bran or cottonseed cake) 25-35%, straw powder 15-20%, domestic animal is held ight soil and grey dregs 55-65%, and calcium superphosphate 1-1.5% adds water and is advisable to hold agglomerating not dripping.PH value nature.
4) bacillusmusilaginosiengineering ACCC10013 bacterial classification is produced
1. the slant strains substratum is:
Sucrose 0.8%-1.2%, K
2HPO
40.04-0.06%, MgSO
47H
2O 0.01-0.03%, MgCl 0.01-0.03%, CaCO
30.8-1.2%, yeast extract paste 0.03-0.05%, agar 1.6-2.2%, pH value 7.1, distilled water 100ml.
2. the solid spawn substratum is:
Wheat bran or cottonseed cake 25-35%, straw powder 15-20%, domestic animal is held ight soil and loam 55-65%, and calcium superphosphate 1-1.5% uses the lime adjust pH, adds water and is advisable to hold agglomerating not dripping.PH value 7.
5) Jingyang streptomycete ACCC40021 bacterial classification is produced
1. the slant strains substratum is:
Potato 15-20%, sugared 1.8-2.2%, NaCl 0.2-0.4%, calcium superphosphate 0.08-0.12%, agar 1.4-1.6%, pH value 7.4-7.9.
2. the solid spawn substratum is:
Rice bran or wheat bran 10-15%, cottonseed cake or soya-bean cake 10-15%, straw powder 10-15%, domestic animal is held ight soil and loam 60-70%, calcium superphosphate 0.8-1.2%, CaCO
30.6-0.9%, pH value 7.4-7.6 adds water and is advisable to hold agglomerating not dripping.
6) blown-ball Azotobacter ACCC10001 bacterial classification is produced
1. the slant strains substratum is:
KNO
30.08-0.12%, K
2HPO
40.04-0.06%, NaCl 0.04-0.06%, Zulkovsky starch 1.8-2.2%, MgSO
47.H
2O 0.04-0.06%, FeSO
47H
2O 0.01%, agar 1.8-2.2%, pH value 7.2-7.4.
2. the solid spawn substratum is:
Rice bran or wheat bran 10-15%, cottonseed cake or soya-bean cake 10-15%, straw powder 10-15%, starch 5-10%, domestic animal is held ight soil and loam 60-70%, calcium superphosphate 1%, pH value 7.4-7.8 adds water and is advisable to hold agglomerating not dripping.
7) the spawn culture processing step is:
Slant strains substratum or solid spawn substratum pack into test tube or triangular flask and incubator are before packing into (test tube is for packing into afterwards), at 1.5kg/cm
2When pressure sterilization 30 minutes, test tube or culture material cool to below 35 ℃, inoculate former bacterial classification, promptly get slant strains and solid spawn cultivating about 5 days about 25 ℃.
(2) separation of bacterial classification, purifying and mutagenesis:
1, material
1) adopt that above-mentioned slant strains substratum separates with it, the bacterial classification of mutagenesis, purifying adapts;
2) experimental ware: 12 secondary diameter 9cm culture dish, some in test tube, one of water-bath, one of spirit lamp, one of transfering loop, some of glass pipettes, one of incubator, transfer room or darkroom.
2, operation steps:
1) substratum is melted;
2) be ready to be diluted in accordance with regulations 10
8Bacterium liquid test tube;
3) left hand is held test tube, will ask for a ring bacterium liquid mixture in the test tube substratum by aseptic technique in sterilisable chamber;
4) micro oscillation substratum makes it and spares;
5) will pour in the culture dish with even bacterium liquid culture medium, slightly shake up, preferably culture dish will be placed on than on the planar desktop, and make substratum thickness roughly the same;
6) ultraviolet radiation mutagenesis and pure strain are cultivated;
Opened the UV-lamp preheating earlier 15 minutes, make light wave stable, then culture dish is inserted transfer room (red light is equipped with in sealing), do not waiting (red light is opened simultaneously) in 30 seconds-2 minutes from the irradiation of quartz burner 25cm place, irradiation finishes culture dish wrapped with black cloth and places in the incubator, cultivating about 7 days under the 23-25 ℃ of temperature, select in growth vigorous energetic bacterial strain access inclined-plane or the test tube substratum and cultivate, separation, mutagenesis flush until turning out, do not have till the bacterial strain of assorted bacterium so repeatedly.
(3) preparation of bio-bacterial manure:
1, the cultural method of bacillus megaterium ACCC10010 and bacillusmusilaginosiengineering ACCC10012 bio-bacterial manure is:
1) substratum: agricultural crop straw powder (15-20 order) 20-30%, domestic animal is held ight soil and loam 60-70%, cottonseed cake or wheat bran 20-30%, calcium superphosphate 1-1.5%, plant ash or coal ash earth 5-10% add the water mixing and are advisable to hold agglomerating not dripping.
2) sterilization and inoculation: after culture material stirs, with 1kg/cm
2Sterilized 30 minutes or normal temperature sterilization after 2 hours cooling insert bacillus megaterium ACCC10010 solid spawn 1-2% and bacillusmusilaginosiengineering ACCC10012 solid spawn 2-3%, with the substratum thorough mixing below 35 ℃.
3) cultivate: the culture material after inoculation is mixed is inserted fermentation machine or shallow-layer fermentation vat or deep ventilation fermentation vat, about 30 ℃ of room temperatures, indoor maintenance certain humidity, the material temperature control is between 30-35 ℃, cultivated 4-5 days, mix bacterial manure by the plate live bacterial count, more than 10,000,000,000/g, be high-quality bacterial manure.
4) oven dry with store: after bacterial manure was cultivated, timely discharging to insert dryer or local method, dry the sealing preservation below 45 ℃ standby.
2, the cultural method of bacillus megaterium ACCC10011 and bacillusmusilaginosiengineering ACCC10013 and Jingyang streptomycete ACCC40021 bio-bacterial manure thereof is:
1) substratum: agricultural crop straw powder (15-20 order) 20-30%, domestic animal is held ight soil and loam 60-70%, cottonseed cake or wheat bran 20-30%, calcium superphosphate 1-1.5%, plant ash or coal ash earth 5-10% add the water mixing and are advisable to hold agglomerating not dripping.
2) sterilization and inoculation: after culture material stirs, with 1kg/cm
2Sterilization or normal temperature sterilization are lowered the temperature after 2 hours below 35 ℃, insert bacillus megaterium ACCC10011 solid spawn 1-2 and bacillusmusilaginosiengineering ACCC10013 solid spawn 1-2% and Jingyang streptomycete ACCC400212-3% and substratum thorough mixing.
3) cultivate: the culture material after inoculation is mixed is inserted fermentation machine or shallow-layer fermentation vat or deep ventilation fermentation vat, about 30 ℃ of room temperatures, indoor maintenance certain humidity, the material temperature control is between 30-35 ℃, cultivated 4-5 days, mix bacterial manure by the plate live bacterial count, more than 15,000,000,000/g, be high-quality bacterial manure.
4) oven dry with store: after bacterial manure was cultivated, timely discharging to insert dryer or local method, dry the sealing preservation below 45 ℃ standby.
The cultural method of blown-ball Azotobacter ACCC10001 and bacillus megaterium ACCC10010 and bacillusmusilaginosiengineering ACCC10012 bio-bacterial manure is:
1) substratum: agricultural crop straw powder (15-20 order) 20-30%, domestic animal is held ight soil and loam 60-70%, cottonseed cake or wheat husband 20-30%, calcium superphosphate 1-1.5%, plant ash or coal ash earth 5-10%, liming with 1% mixes is advisable to hold agglomerating not dripping, and substratum is piled up sealing spontaneous fermentation 48 hours.
2) inoculation: the substratum spreading for cooling after the accumulation spontaneous fermentation is mixed thoroughly, insert blown-ball Azotobacter ACCC10001 solid spawn 3-5% and bacillus megaterium ACCC10010 5-8% and bacillusmusilaginosiengineering ACCC10012 solid spawn 5-6% and substratum thorough mixing then.
3) cultivate: the culture material after inoculation is mixed is inserted fermentation machine or shallow-layer fermentation vat or deep ventilation fermentation vat, about 30 ℃ of room temperatures, indoor maintenance certain humidity, the material temperature control is between 30-35 ℃, cultivated 4-5 days, mix bacterial manure by the plate live bacterial count, more than 10,000,000,000/g, be high-quality bacterial manure.
4) oven dry with store: after bacterial manure was cultivated, timely discharging to insert dryer or local method, dry the sealing preservation below 45 ℃ standby.
(4) bacterial manure plate live bacterial count method:
1) operation steps:
Get above-mentioned bacterial manure 10g, add sterilized water 90ml, 50 of granulated glass sphere or coarse sand granules fully shook 5-10 minute, promptly got 1/10 product diluent, draw 1 milliliter of this diluent with suction pipe again, add in the test tube of containing 9 ml sterile waters, make 1% diluent, method can be diluted to 1/10000000th according to this, (multiple of dilution must decide on the general bacteria containing amount of product, and 30-100 bacterium colony of each plate generation of general requirement is for well so that 1/100000000th.Get then and be diluted to the diluent 1ml that finally requires concentration, pour into and fill that 15-20ml is chilled to non-scald on hand but in the plate substratum that do not solidify, generally can do three repetitions, fully shake up.Be placed upside down in suitable the giving birth under the temperature of this check bacterium after the cooling and cultivate, treat to count after plate grows bacterium colony.
2) calculation formula
Every g product contains the average colony number * extension rate of bacterium number=plate
(5) cooperation of inorganic raw material nitrogen, phosphorus, potassium, trace element:
1) prescription:
1. 2. fused(calcium magnesium)phosphate 15-18% of urea 34-36%
3. 4. Repone K 30-35% of calcium superphosphate 15-18%
5. 6. lime carbonate 2-4% of sal epsom 2-4%
7. 8. zinc sulfate 0.2-0.3% of boric acid 0.1-0.2%
9. 10. ammonium molybdate 0.01-0.03% of manganous sulfate 0.3-0.4%
copper sulfate 1-1.2% Xanthohumate 0.1-0.2%
rare earth 0.08-0.1%
2) prepare and mix:
Above-mentioned inorganic raw material is mixed, pulverized the 15-20 order, pack standby or directly cooperate composite fertilizer.
(6) preparation of organic raw material and prescription:
1) prescription:
1. agricultural crop straw (15-20 order) 25-30% 2. domestic animal hold poultry manure 55-65%
3. active pond sludge 15-20% is plant ash or cinder ash 5-10% 4.
5. lime 1-2%
2) preparation:
Above material thorough mixing is even, and humidity is advisable to hold agglomerating not dripping, and piles up sealing spontaneous fermentation more than 96 hours, spreads out to dry or dry and pulverizes the 15-20 order and store standby or directly cooperate composite fertilizer.
(7) prescription of composite fertilizer and preparation are divided into three groups:
1, mineral manure 45-50%, organic corruption thing 45-50%, the bacteria agent bacillus megaterium phosphorus bacteria ACCC10010 bacterial manure 5% of adding, bacillusmusilaginosiengineering ACCC10013 bacterial manure 5%; Processing step is: microbial inoculum is added in the mixing machine, add raw materials such as other inorganic fertilizer, stir, the oven dry of granulating.
2, mineral manure 45-50%, organic corruption thing 45-50%; Add bacteria agent bacillus megaterium ACCC10011 bacterial manure 4%, bacillusmusilaginosiengineering ACCC10013 bacterial manure 4%, Jingyang streptomycete ACCC40021 bacterial manure 2%; Processing step is: bacterial manure is added in the mixing machine, add raw materials such as inorganic fertilizer, stir, the oven dry of granulating.
3, mineral manure 40-45%; Organic corruption thing 40-45%; Add bio-bacterial manure blown-ball Azotobacter ACCC10001 2%, bacillus megaterium ACCC10010 bacterial manure 4%, Jingyang strepto-bacterial manure ACCC10022 4%; Processing step is: bacterial manure is added in the mixing machine, add raw materials such as inorganic fertilizer, stir the oven dry of granulating.
Specific examples:
(1) bacillus megaterium ACCC10010 and bacillusmusilaginosiengineering ACCC10012 compound bacterial fertilizer, and add mineral manure, organic fertilizer, fitting in the compound granulated fertilizer of many bacterium organic-inorganic, its operation steps is as follows:
1, compound bacterial fertilizer solid fermentation and technology:
1) substratum: 1. agricultural crop straw powder (cross 15 orders) 20%, 2. domestic animal is held ight soil and loam 50%, 3. cottonseed cake or wheat bran 20%, 4. calcium superphosphate 1.5%, and 5. plant ash or coal ash 8.5% add water and mix and be advisable to hold agglomerating not dripping;
2) sterilization and inoculation: after culture material stirs, with 1kg/cm
2Cooling is below 35 ℃ after 2 hours for sterilization or normal temperature sterilization, and access bacillus megaterium ACCC10011 solid spawn 2% and bacillusmusilaginosiengineering ACCC10012 solid spawn 2% are with the substratum thorough mixing;
3) cultivate: the culture material after inoculation is mixed is inserted fermentation machine or shallow-layer fermentation vat or deep ventilation fermentation vat, about 30 ℃ of room temperatures, indoor maintenance certain humidity, the material temperature control is between 30-35 ℃, cultivated 4-5 days, fermentation materials more than 15,000,000,000/g, is high-quality bacterial manure by the plate live bacterial count;
4) oven dry with store: after bacterial manure was cultivated, timely discharging to insert dryer or local method, dry the sealing preservation below 45 ℃ standby or directly prepare composite fertilizer.
2, the prescription of inorganic fertilizer raw material and preparation:
1) prescription:
1. 2. fused(calcium magnesium)phosphate 15% of urea 34%
3. 4. Repone K 30% of calcium superphosphate 15%
5. 6. lime carbonate 2% of sal epsom 2%
7. 8. zinc sulfate 0.2% of boric acid 0.1%
9. 10. ammonium molybdate 0.01% of manganous sulfate 0.3%
copper sulfate 1-1% Xanthohumate 0.2%
rare earth 0.09%
2) be mixed with: the inorganic raw material thorough mixing was pulverized 15 orders, the standby or configuration composite fertilizer of pack sealing.
3, the prescription of fertilizer raw material and preparation
1) prescription:
1. agricultural crop straw (15 order) 25% 2. domestic animal hold poultry manure 55%
3. active pond sludge 1 4% is plant ash or cinder ash 5% 4.
5. lime 1%
2) preparation:
Above material thorough mixing is even, and humidity is advisable to hold agglomerating not dripping, and piles up sealing spontaneous fermentation more than 96 hours, spreads out to dry or dry and pulverizes the 15-20 order and store standby.
4, the prescription of the compound granulated fertilizer of many bacterium biologic organic, inorganic and preparation:
1) prescription:
1. bacillus megaterium ACCC10010 and bacillusmusilaginosiengineering ACCC10012 compound bacterial fertilizer 10%
2. the inorganic fertilizer raw material 35%
3. organic fertilizer 55%
2) be mixed with granulation:
Earlier compound bacterial fertilizer is inserted in the mixer and mix with organic fertilizer; again inorganic material is added in the mixer and mix; the compound manure that mixes is entered the tablets press granulation by transfer roller, and oven drying at low temperature promptly becomes mixed culture biological organic inorganic compound granulated fertilizer.
(2) bacillus megaterium ACCC10011 and bacillusmusilaginosiengineering ACCC10013 and Jingyang streptomycete ACCC40021 compound bacterial fertilizer, and add mineral manure, organic fertilizer, fitting in mixed culture biological organic inorganic compound granulated fertilizer, its operation steps is as follows:
1, compound bacterial fertilizer solid fermentation and technology:
1) substratum:
1. agricultural crop straw (cross 15 orders) 20% 2. domestic animal hold poultry manure 50%
3. 4. calcium superphosphate 1.5% of cottonseed cake or wheat bran 20%
5. plant ash or coal ash 8.5%
Adding the water mixing is advisable to hold agglomerating not dripping.
2) sterilization and inoculation: after culture material stirs, with 1kg/cm
2Sterilized 30 minutes or normal temperature sterilization after 2 hours cooling insert bacillus megaterium ACCC10011 solid spawn 1% and bacillusmusilaginosiengineering ACCC10013 solid spawn 1% and Jingyang streptomycete ACCC40021 2% and substratum thorough mixing below 35 ℃.
3) cultivate: the culture material after inoculation is mixed is inserted fermentation machine or shallow-layer fermentation vat or deep ventilation fermentation vat, about 30 ℃ of room temperatures, indoor maintenance certain humidity, the material temperature control is between 30-35 ℃, cultivated 4-5 days, mixed bacterium more than 15,000,000,000/g, is high-quality bacterial manure by the plate live bacterial count.
4) oven dry with store: after bacterial manure was cultivated, timely discharging to insert dryer or local method, dry the sealing preservation below 45 ℃ standby.
2, the prescription of inorganic fertilizer raw material and preparation
1) prescription:
1. 2. fused(calcium magnesium)phosphate 15% of urea 34%
3. 4. Repone K 30% of calcium superphosphate 15%
5. 6. lime carbonate 2% of sal epsom 2%
7. 8. zinc sulfate 0.2% of boric acid 0.1%
9. 10. ammonium molybdate 0.01% of manganous sulfate 0.3%
copper sulfate 1-1% Xanthohumate 0.2%
rare earth 0.09%
2) prepare and mix:
Above-mentioned inorganic raw material is mixed, pulverized the 15-20 order, pack standby.
3, the prescription of fertilizer raw material and preparation
1) prescription:
1. agricultural crop straw (15 order) 25 2. domestic animal hold poultry manure 55%
3. active pond sludge 14% is plant ash or cinder ash 5% 4.
5. lime 1%
2) preparation:
Above material thorough mixing is even, and humidity is advisable to hold agglomerating not dripping, and piles up sealing spontaneous fermentation more than 96 hours, spreads out to dry or dry and pulverizes the 15-20 order and store standby.
4, the prescription of the compound granulated fertilizer of many bacterium biologic organic, inorganic and preparation:
1) prescription:
1. bacillus megaterium ACCC10011 and bacillusmusilaginosiengineering ACCC10012 and Jingyang streptomycete ACCC42001 compound bacterial fertilizer 10%
2. the inorganic fertilizer raw material 35%
3. organic fertilizer 55%
2) preparation mixing granulation:
Earlier compound bacterial fertilizer is inserted in the mixer and mix with organic fertilizer; again inorganic material is added in the mixer and mix; the compound manure that mixes is entered the tablets press granulation by transfer roller, and oven drying at low temperature promptly becomes mixed culture biological organic inorganic compound granulated fertilizer.
(3) blown-ball Azotobacter ACCC10001 and bacillus megaterium ACCC10010 and bacillusmusilaginosiengineering ACCC10012 compound bacterial fertilizer, and add mineral manure, organic fertilizer, fitting in mixed culture biological organic inorganic compound granulated fertilizer, its operation steps is as follows:
1, compound bacterial fertilizer solid fermentation process:
1) substratum:
1. agricultural crop straw (cross 15 orders) 20%, 2. domestic animal is held ight soil and loam 50%, 3. cottonseed cake or wheat bran 20%, 4. calcium superphosphate 1.5%, 5. plant ash or coal ash 8.5%
Above raw material adds the water mixing and is advisable to hold agglomerating not dripping.
2) inoculation: the substratum spreading for cooling after the accumulation spontaneous fermentation is mixed thoroughly, insert blown-ball Azotobacter ACCC10001 solid spawn 4% and bacillus megaterium ACCC10010 5% and bacillusmusilaginosiengineering ACCC10012 solid spawn 6% and substratum thorough mixing then.
3) cultivate: the culture material after inoculation is mixed is inserted fermentation machine or shallow-layer fermentation vat or deep ventilation fermentation vat, about 30 ℃ of room temperatures, indoor maintenance certain humidity, the material temperature control is between 30-35 ℃, cultivated 4-5 days, mixed bacterium more than 10,000,000,000/g, is high-quality bacterial manure by the plate live bacterial count.
4) oven dry with store: after bacterial manure was cultivated, timely discharging to insert dryer or local method, dry the sealing preservation below 45 ℃ standby.
2, the prescription of inorganic fertilizer raw material and preparation
1) prescription:
1. 2. fused(calcium magnesium)phosphate 15% of urea 34%
3. 4. Repone K 30% of calcium superphosphate 15%
5. 6. lime carbonate 2% of sal epsom 2%
7. 8. zinc sulfate 0.2% of boric acid 0.1%
9. 10. ammonium molybdate 0.01% of manganous sulfate 0.3%
copper sulfate 1-1% Xanthohumate 0.2%
rare earth 0.09%
2) prepare and mix:
Above-mentioned inorganic raw material is mixed, pulverized the 15-20 order, pack standby.
3, the prescription of fertilizer raw material and preparation
1) composition of raw materials: 1. agricultural crop straw (15 order) 25% 2. domestic animal hold poultry manure 55%
3. active pond sludge 14% is plant ash or cinder ash 5% 4.
5. lime 1%
2) prepare and mix:
Above material thorough mixing is even, and humidity is advisable to hold agglomerating not dripping, and piles up sealing spontaneous fermentation more than 96 hours, spreads out to dry or dry and pulverizes the 15-20 order and store standby.
4, the prescription of the compound granulated fertilizer of many bacterium biologic organic, inorganic and preparation:
1) prescription:
1. blown-ball Azotobacter ACCC10001 and bacillus megaterium ACCC10010 and bacillusmusilaginosiengineering ACCC10012 compound bacterial fertilizer 10%;
2. the inorganic fertilizer raw material 35%
3. organic fertilizer 55%
2) preparation mixing granulation:
Earlier compound bacterial fertilizer is inserted in the mixer and mix with organic fertilizer; again inorganic material is added in the mixer and mix; the compound manure that mixes is entered the tablets press granulation by transfer roller, and oven drying at low temperature promptly becomes mixed culture biological organic inorganic compound granulated fertilizer.
Need to prove and be numbered China Committee for Culture Collection of Microorganisms's agricultural microorganism center preserving number (Chinese agriculture bacterial classification catalogue version in 1991) behind the strain name.
In this case, the fermentation machine, fermentor tank, fermentation vat are the container of solid or liquid fermenting bacterial classification or product, have essentially identical effect according to different conditions.
Table 1 active constituent content
| Project | Standard | Remarks |
| Nitrogen | ??＞6％ | |
| Phosphorus | ??＞5％ | |
| Potassium | ??＞4％ | |
| Organic | ??＞45％ | |
| Total bacterium hundred million/g | ＞2 hundred million/g | |
| The trace element summation | ??＞4％ | |
| Moisture content | ??10-12％ | |
| The pH value | ??6.4-7.2 |
Table 2 product controlled trial effect (being used for corn topdresses)
| Title | Consumption/mu | Price/mu | Output/mu | Units increased in production | Stimulation ratio |
| Many bacterium composite fertilizers | ??40kg | 56 yuan | ????670 | ??150kg | ??34％ |
| Per tonly press 1400 yuan | |||||
| Unit's nitrogen rephosphorization mixes potash fertilizer | ??30kg | 57 yuan | ????620 | ??120kg | ??24％ |
| Per tonly press 1900 yuan | |||||
| Contrast | Do not apply fertilizer | ????500 |
Claims (14)
1, a kind of production method of many bacterium biological organic inorganic compound fertilizer comprises separation, purifying mutagenesis, the preparation of bacterium culture medium and the preparation of bacterial classification of bacterial classification, the fermentative production of bacterial manure; The quality examination of bacterial manure; The preparation of mineral manure and prescription; The organic raw material corruption is prepared and preparation; The cooperation of composite fertilizer and granulation and oven dry.
Wherein the batching of composite fertilizer is:
The 15-30 order was pulverized in the fertile oven dry of cultured multi-strain bacteria, with bio-bacterial manure 10-15%, mineral manure 45-50%, organic corruption thing 45-50%.
The bacterial classification that bio-bacterial manure is produced is:
Bacillus megaterium ACCC10010 microbial inoculum 4-8% and bacillusmusilaginosiengineering ACCC10012 microbial inoculum 4-8% compatibility; Or
Bacillus megaterium ACCC10011 microbial inoculum 4-8% and bacillusmusilaginosiengineering ACCC10013 microbial inoculum 4-8% and Jingyang streptomycete ACCC40021 microbial inoculum 2-4% compatibility; Or
Blown-ball Azotobacter ACCC10001 microbial inoculum 4-8% and bacillus megaterium ACCC10010 microbial inoculum 4-8% and bacillusmusilaginosiengineering ACCC10012 2-4% compatibility;
More than the microbial inoculum of various compatibilities add organic corruption thing 45-50%, inorganic fertilizer 45-50% again, according to quantity bacterial manure is added in the mixing machine, mix, organic and inorganicly wait granulation oven dry behind raw material thorough mixing with other.
2, the production method of the many bacterium composite fertilizers of biology according to claim 1, it is characterized in that above-mentioned bacterial manure 10-15%, mineral manure 45-50%, organic corruption thing 45-50%, the bacteria agent bacillus megaterium phosphorus bacteria ACCC10010 bacterial manure 5% that adds, bacillusmusilaginosiengineering ACCC10013 bacterial manure 5%; Processing step is: microbial inoculum is added in the mixing machine, add raw materials such as other fertilizer, stir, the oven dry of granulating.
3, the production method of the many bacterium composite fertilizers of biology according to claim 1 is characterized in that above-mentioned bacterial manure 10-15%, mineral manure 45-50%, organic corruption thing 45-50%; Add bacteria agent bacillus megaterium ACCC10011 bacterial manure 4%, bacillusmusilaginosiengineering ACCC10013 bacterial manure 4%, Jingyang streptomycete ACCC40021 bacterial manure 2%; Processing step is: bacterial manure is added in the mixing machine, add raw materials such as fertilizer, stir, the oven dry of granulating.
4, many fungi compound fertilizers of biology production method according to claim 1 is characterized in that above-mentioned bacterial manure 5-10%, mineral manure 40-45%; Organic corruption thing 40-45%; Add bio-bacterial manure blown-ball Azotobacter ACCC10001 2%, bacillus megaterium ACCC10010 bacterial manure 4%, Jingyang strepto-bacterial manure ACCC10022 4%; Processing step is: bacterial manure is added in the mixing machine, add raw materials such as fertilizer, stir the oven dry of granulating.
5, the production method of the many fungi compound fertilizers of biology according to claim 2 is characterized in that the separation and the purifying of above-mentioned bacterial classification:
1) bouillon media is melted;
2) will melt test tube dries;
3) left hand is held test tube, in aseptic processing room or darkroom, will ask for the above-mentioned bacterium liquid mixture of a ring in substratum by aseptic technique;
4) micro oscillation substratum makes it and spares;
5) will pour in the culture dish with the substratum of even sample to be separated, shake up,, former bacteria culture fluid can be diluted in advance if cell count is excessive in the bacterium liquid;
6) uviolizing processing and pure strain are cultivated:
Open ultraviolet preheating 15 minutes earlier, make light wave stable, then culture dish is inserted darkroom or transfer room, from about UV-lamp 20cm, locating to shine 30 seconds to 2 minutes (deciding) on the bacterial strain difference, open red light simultaneously, then culture dish is wrapped with black cloth and inserted in the incubator, under 28-30 ℃ of constant temperature, cultivated 24-48 hour, select in the vigorous energetic bacterial strain access of the growth test tube slant substratum and cultivate, separate several times so repeatedly, in test tube slant or culture dish, do not have assorted bacterium, strain growth vigorous energetic till, insert then and cultivate preservation in the slant tube or as producing bacterial classification.
6, the production method of the many bacterium composite fertilizers of biology according to claim 1 and 2 is characterized in that above-mentioned:
1) bacillus megaterium ACCC10010 bacterial classification is produced, and its slant medium is: albumen freezes 0.4-0.6%, NaCl 0.4-0.6%, extractum carnis 0.2-0.4%, agar 1.3-1.6%, distilled water 100ml, pH value nature; The solid spawn substratum is: rice bran (wheat bran or cottonseed cake) 25-35%, straw powder 10-15%, domestic animal hold class just with grey dregs 60-70%, calcium superphosphate 1-1.5% adds water and is advisable to hold agglomerating not dripping.
2) bacillusmusilaginosiengineering ACCC10012 bacterial classification is produced, and its slant medium is: liver immersion liquid 100ml, NaCl 0.4-0.6%, peptone 0.8-1.2%, agar 1.8-2.3%, pH value 7; The solid spawn substratum is: wheat bran or cottonseed cake 25-35%, and straw powder 15-20%, domestic animal is held ight soil and loam 55-65%, calcium superphosphate 1-1.5%, pH value 7 adds water and is advisable to hold agglomerating not dripping.
3) the spawn culture processing step is:
The interior 1.5klg/cm of test tube, triangular flask or gunnysack packs into behind the substratum thorough mixing
2When pressure sterilization 30 minutes, culture material cooled to below 35 ℃, inoculation culture promptly got bacterial classification about 5 days.
7, according to the production method of claim 1 or 3 described many bacterium biologic compound fertilizers, it is characterized in that above-mentioned:
1) bacillus megaterium ACCC10011 bacterial classification is produced, and its slant medium is: potato vat liquor 50ml, sucrose 1.5-2.5%, agar 1.5-2.5%, distilled water 50ml, pH value nature; The solid spawn substratum is: rice bran (wheat bran or cottonseed cake) 25-35%, and straw powder 15-20%, domestic animal is held ight soil and grey dregs 55-65%, and calcium superphosphate 1-1.5% regulates the pH value with lime, adds water and is advisable to hold agglomerating not dripping.
2) bacillusmusilaginosiengineering ACCC10013 bacterial classification is produced its slant medium and is: sucrose 0.8%-1.2%, K
2HPO
40.04-0.06%, MgSO
47H
2O 0.01-0.03%, MgCl 0.01-0.03%, CaCO
30.8-1.2%, yeast extract paste 0.03-0.05%, agar 1.6-2.2%, pH value 7.1, distilled water 100ml; The solid spawn substratum is: wheat bran or cottonseed cake 25-35%, and straw powder 15-20%, domestic animal is held ight soil and loam 55-65%, and calcium superphosphate 1-1.5% uses the lime adjust pH, adds water and is advisable to hold agglomerating not dripping.
3) Jingyang streptomycete ACCC40021 bacterial classification production slant medium is: potato 15-20%, sugared 1.8-2.2%, NaCl 0.2-0.4%, calcium superphosphate 0.08-0.12%, agar 1.4-1.6%, pH value 7.4-7.9; The solid spawn substratum is: rice bran or wheat bran 10-15%, and cottonseed cake or soya-bean cake 10-15%, straw powder 10-15%, domestic animal is held ight soil and loam 60-70%, calcium superphosphate 0.8-1.2%, CaCO
30.6-0.9%, pH value 7.4-7.6 adds water and is advisable to hold agglomerating not dripping.
4) the bacterial classification production craft step is: 1.5kg/cm in pack into behind the substratum thorough mixing test tube, triangular flask or the gunnysack
2When pressure sterilization 30 minutes, culture material cool to below 35 ℃, promptly got bacterial classification in inoculation culture 5-6 days.
8, according to claim 1 or 4 described many fungi compound fertilizers of biology production methods, it is characterized in that above-mentioned:
1) blown-ball Azotobacter ACCC10001 bacterial classification is produced, and its slant medium is: KNO
30.08-0.12%, K
2HPO
40.04-0.06%, NaCl 0.04-0.06%, Zulkovsky starch 1.8-2.2%, MgSO
47.H
2O 0.04-0.06%, FeSO
47H
2O 0.01%, agar 1.8-2.2%, pH value 7.2-7.4; The solid spawn substratum is: rice bran or wheat bran 10-15%, and cottonseed cake or soya-bean cake 10-15%, straw powder 10-15%, starch 5-10%, domestic animal is held ight soil and loam 60-70%, calcium superphosphate 1%, pH value 7.4-7.8 adds water and is advisable to hold agglomerating not dripping.
2) bacillus megaterium ACCC10010 bacterial classification is produced, and its slant strains substratum is: albumen freezes 0.4-0.6%, NaCl 0.4-0.6%, extractum carnis 0.2-0.4%, agar 1.6-1.8%, distilled water 100ml, pH value 7; The solid spawn substratum is: rice bran (wheat bran or cottonseed cake) 25-35%, and straw powder 10-15%, domestic animal is held ight soil and grey dregs 60-70%, and calcium superphosphate 1-1.5% is with liming adjust pH (the pH value is 7).
3) bacillusmusilaginosiengineering ACCC10012 bacterial classification is produced, and its slant medium is: liver immersion liquid 100ml, NaCl 0.4-0.6%, peptone 0.8-1.2%, agar 1.8-2.3%, pH value 7; The solid spawn substratum is: wheat bran or cottonseed cake 25-35%, and straw powder 15-20%, domestic animal is held ight soil and loam 55-65%, calcium superphosphate 1-1.5%, pH value 7 adds water and is advisable to hold agglomerating not dripping.
4) the spawn culture processing step is: behind the substratum thorough mixing, and 1.5kg/cm in the test tube of packing into, triangular flask or the gunnysack
2When pressure sterilization 30 minutes, culture material cooled to below 35 ℃, inoculation culture promptly got bacterial classification about 5 days.
9,, it is characterized in that the cultural method of above-mentioned bacillus megaterium ACCC10010 and bacillusmusilaginosiengineering ACCC10012 bio-bacterial manure is according to the production method of claim 1 or 2 or 6 described many bacterium biological organic inorganic compound fertilizers:
1) substratum: agricultural crop straw powder (15-20 order) 20-30%, domestic animal is held ight soil and loam 60-70%, cottonseed cake or wheat bran 20-30%, calcium superphosphate 1-1.5%, plant ash or coal ash earth 5-10% add the water mixing and are advisable to hold agglomerating not dripping.
2) sterilization and inoculation: after culture material stirs, with 1kg/cm
2Sterilized 30 minutes or normal temperature sterilization after 2 hours cooling insert bacillus megaterium ACCC10010 solid spawn 1-2% and bacillusmusilaginosiengineering ACCC10012 solid spawn 2-3%, with the substratum thorough mixing below 35 ℃.
3) cultivate: the culture material after inoculation is mixed is inserted fermentation machine or shallow-layer fermentation vat or deep ventilation fermentation vat, about 30 ℃ of room temperatures, indoor maintenance certain humidity, the material temperature control is between 30-35 ℃, cultivated 4-5 days, mixed bacterium more than 10,000,000,000/g, is high-quality bacterial manure by the plate live bacterial count.
4) oven dry with store: after bacterial manure was cultivated, timely discharging to insert dryer or local method, dry the sealing preservation below 45 ℃ standby.
10,, it is characterized in that the cultural method of above-mentioned bacillus megaterium ACCC10011 and bacillusmusilaginosiengineering ACCC10013 and Jingyang streptomycete ACCC40021 bio-bacterial manure thereof is according to the production method of claim 1 or 3 or 7 described many bacterium biologic organic, inorganic composite fertilizers:
1) substratum: agricultural crop straw powder (15-20 order) 20-30%, domestic animal is held ight soil and loam 60-70%, cottonseed cake or wheat bran 20-30%, calcium superphosphate 1-1.5%, plant ash or coal ash earth 5-10% add the water mixing and are advisable to hold agglomerating not dripping.
2) sterilization and inoculation: after culture material stirs, with 1kg/cm
2Sterilization or normal temperature sterilization are lowered the temperature after 2 hours below 35 ℃, insert bacillus megaterium ACCC10011 solid spawn 1-2% and bacillusmusilaginosiengineering ACCC10013 solid spawn 1-2% and Jingyang streptomycete ACCC40021 2-3% and substratum thorough mixing.
3) cultivate: the culture material after inoculation is mixed is inserted fermentation machine or shallow-layer fermentation vat or deep ventilation fermentation vat, about 30 ℃ of room temperatures, indoor maintenance certain humidity, the material temperature control is between 30-35 ℃, cultivated 4-5 days, mix bacterial manure by the plate live bacterial count, more than 15,000,000,000/g, be high-quality bacterial manure.
4) oven dry with store: after bacterial manure was cultivated, timely discharging to insert dryer or local method, dry the sealing preservation below 45 ℃ standby.
11,, it is characterized in that the cultural method of above-mentioned blown-ball Azotobacter ACCC10001 and bacillus megaterium ACCC10010 and bacillusmusilaginosiengineering ACCC10012 bio-bacterial manure is according to the production method of claim 1 or 4 or 8 described many bacterium biological organic inorganic compound fertilizers:
1) substratum: agricultural crop straw powder (15-20 order) 20-30%, domestic animal is held ight soil and loam 60-70%, cottonseed cake or wheat husband 20-30%, calcium superphosphate 1-1.5%, plant ash or coal ash earth 5-10%, liming with 1% mixes is advisable to hold agglomerating not dripping, and substratum is piled up sealing spontaneous fermentation 48 hours.
2) inoculation: the substratum spreading for cooling after the accumulation spontaneous fermentation is mixed thoroughly, insert blown-ball Azotobacter ACCC10001 solid spawn 3-5% and bacillus megaterium ACCC10010 5-8% and bacillusmusilaginosiengineering ACCC10012 solid spawn 5-6% and substratum thorough mixing then.
3) cultivate: the culture material after inoculation is mixed is inserted fermentation machine or shallow-layer fermentation vat or deep ventilation fermentation vat, about 30 ℃ of room temperatures, indoor maintenance certain humidity, the material temperature control is between 30-35 ℃, cultivated 4-5 days, mixed bacterium more than 10,000,000,000/g, is high-quality bacterial manure by the plate live bacterial count.
4) oven dry with store: after bacterial manure was cultivated, timely discharging to insert dryer or local method, dry the sealing preservation below 45 ℃ standby.
12, according to claim 1 or 9 or the production method of 10 or 11 described many bacterium biological organic inorganic compound fertilizers, it is characterized in that above-mentioned bacterial manure plate live bacterial count method is:
Get above-mentioned bacterial manure 10g, add sterilized water 90ml, 50 of granulated glass sphere or coarse sand granules fully shook 5-10 minute, promptly got 1/10 product diluent, draw 1 milliliter of this diluent with suction pipe again, add in the test tube of containing 9 ml sterile waters, make 1% diluent, method can be diluted to 1/10000000th according to this, (multiple of dilution must decide on the general bacteria containing amount of product, and 30-100 bacterium colony of each plate generation of general requirement is for well so that 1/100000000th.Get then and be diluted to the diluent 1ml that finally requires concentration, pour into and fill that 15-20ml is chilled to non-scald on hand but in the plate substratum that do not solidify, generally can do three repetitions, fully shake up.Be placed upside down in suitable the giving birth under the temperature of this check bacterium after the cooling and cultivate, treat to count after plate grows bacterium colony.Its calculation formula is:
Every g product contains the average colony number * extension rate of bacterium number=plate.
13, the production method of many bacterium biological organic inorganic compound fertilizer according to claim 1 is characterized in that the preparation of above-mentioned mineral manure and prescription are:
1) prescription:
1. 2. fused(calcium magnesium)phosphate 15-18% of urea 34-36%
3. 4. Repone K 30-35% of calcium superphosphate 15-18%
5. 6. lime carbonate 2-4% of sal epsom 2-4%
7. 8. zinc sulfate 0.2-0.3% of boric acid 0.1-0.2%
9. 10. ammonium molybdate 0.01-0.03% of manganous sulfate 0.3-0.4%
copper sulfate 1-1.2% Xanthohumate 0.1-0.2%
rare earth 0.08-0.1%
2) prepare and mix:
Above-mentioned inorganic raw material is mixed, pulverized the 15-20 order, pack standby.
14, the production method of many bacterium biological organic inorganic compound fertilizer according to claim 1 is characterized in that the preparation of above-mentioned organic raw material and prescription are:
1) prescription:
1. agricultural crop straw (15-20 order) 25-30% 2. domestic animal hold poultry manure 55-65%
3. active pond sludge 15-20% is plant ash or cinder ash 5-10% 4.
5. lime 1-2%
2) preparation:
Above material thorough mixing is even, and humidity is advisable to hold agglomerating not dripping, and piles up sealing spontaneous fermentation more than 96 hours, spreads out to dry or dry and pulverizes the 15-20 order and store standby.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96114316 CN1165127A (en) | 1996-12-13 | 1996-12-13 | Method for producing mixed culture biological organic inorganic compound granulated fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96114316 CN1165127A (en) | 1996-12-13 | 1996-12-13 | Method for producing mixed culture biological organic inorganic compound granulated fertilizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1165127A true CN1165127A (en) | 1997-11-19 |
Family
ID=5122075
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 96114316 Pending CN1165127A (en) | 1996-12-13 | 1996-12-13 | Method for producing mixed culture biological organic inorganic compound granulated fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1165127A (en) |
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| CN1099394C (en) * | 1999-11-12 | 2003-01-22 | 高明金葵子植物营养有限公司 | Microbe-P-K fertilizer and its preparing process |
| WO2009003341A1 (en) * | 2007-07-04 | 2009-01-08 | Qingli Yang | Composite chemical fertilizer comprising bioactive materials |
| CN101962622B (en) * | 2009-07-24 | 2013-06-26 | 许民强 | Complex bacteria inoculant for preparing organic fertilizer from effluents of animal slaughter houses and breeding plants serving as raw materials and preparation method thereof |
| US20110247378A1 (en) * | 2009-11-13 | 2011-10-13 | Begley Paul V | Low-Carbon Fertilizers and Processes for Producing the Same |
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