CN102442865A - Functional bacterial manure and preparation method thereof - Google Patents
Functional bacterial manure and preparation method thereof Download PDFInfo
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- CN102442865A CN102442865A CN2011102909250A CN201110290925A CN102442865A CN 102442865 A CN102442865 A CN 102442865A CN 2011102909250 A CN2011102909250 A CN 2011102909250A CN 201110290925 A CN201110290925 A CN 201110290925A CN 102442865 A CN102442865 A CN 102442865A
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- bacillusmusilaginosiengineering
- microbial inoculum
- streptomyces fradiae
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- bacterial manure
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Abstract
The invention discloses a functional bacterium agent or functional bacterial manure containing streptomyces fradiae with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.5133 and bacillus mucilaginosus. According to the preparation method disclosed by the invention, the streptomyces fradiae and the bacillus mucilaginosus are subjected to liquid-solid combined fermentation to obtain a bacterium agent; and the bacterium agent and manure are prepared according to a formula for application. According to the functional bacterial manure disclosed by the invention, on the basis of favorable disease resistance and capabilities of providing metabolites such as auxin and the like as well as fixing nitrogen and solubilizing phosphor of the streptomyces fradiae, the streptomyces fradiae is matched with the bacillus mucilaginosus to obtain favorable potassium-releasing performance, thereby forming scientific strain collocation.
Description
Technical field
The invention belongs to and be used for the soil and fertilizer field, be specifically related to a kind of agricultural microorganism preparation and application thereof.
Background technology
Agriculture prodn needs soil that the nutritive substance and the living environment of plant growth are provided.For many years, using of chemical fertilizer played keying action to getting agricultural profit.Yet; Because long-term application chemical fertilizer, bio-bacterial manure and fertilizer are under-supply, all kinds of nutrients ratio imbalances; Cause farmland ecological environment, soil physical and chemical property and soil microflora to receive destruction in various degree, influenced the quality of agricultural-food to a certain extent.Therefore, chemical fertilizer pollution has become the world today's one big public hazards, and the present soil resource present situation of China presses for through the people to measure replenishes the soil organism and beneficial microbe colony, guarantees agricultural planting level and crop products quality.
In ten big countermeasures of China's ecological environmental protection, clearly advocate and to promote " ecological agriculture "; Preserve the ecological environment and agricultural land soil; Promote the production of pollution-free healthy food; Assurance has the sufficient amount organic matter to return soil, to promote biological cycle in the ecosystem, reaches maintenance and strengthens soil fertility and biological activity purpose.Bio-feritlizer (comprising that mikrobes such as microbial inoculum, bacterial manure are the product of the main mechanism of action) can play and prevent and treat disease and nontoxic to people and animals, is the preferred production means that the high-quality non-polluted farm product is produced.Fast development along with China's modern agriculture; Particularly the green organic agricultural cultivated area constantly enlarges; The specified microorganisms bacterium is dissolved into fertilizer, produce to soil useful and function more comprehensively fertilizer product become the emphasis of current fertiliser prodn enterprise development.
Microbial inoculum/bacterial fertilizer products of selling in the market is a principal feature with raising the output and culture fertility mainly; The major technique technology of its product is to adopt solid adsorbent to adsorb behind the liquid fermenting, and on pertinent literature and market, does not find the functional microbial inoculum product processed through liquid-solid combined ferment technology by streptomyces fradiae and bacillusmusilaginosiengineering as yet.Though existing liquid fermenting absorbing process production process is simple, its bacterium number and biological activity are unsatisfactory.
Summary of the invention
The present invention at first provides a kind of functional microbial inoculum that contains streptomyces fradiae CGMCC No.5133 and bacillusmusilaginosiengineering.
Another object of the present invention also is to provide the functional bacterial manure that contains streptomyces fradiae CGMCC No.5133 and bacillusmusilaginosiengineering.
Streptomyces fradiae provided by the invention (Streptomyces fradiae) CLIMAX-VSF separates from the soil of high-yield field and get, and this bacterial strain is very disease-resistant significantly, fixed nitrogen phosphorus decomposing and secrete the growth hormone performance.(the address: No. 3, A, DaTun Road, Chaoyang District, BeiJing City at China Committee for Culture Collection of Microorganisms common micro-organisms center; Institute of Microorganism, Academia Sinica; Postcode 100101) carry out preservation, the preservation accession designation number is CGMCC No.5133, and preservation date is on August 12nd, 2010.
The functional microbial inoculum that provides of the present invention, contain the component of following weight part:
Mixture 5-70 part of streptomyces fradiae CGMCC No.5133 microbial inoculum and bacillusmusilaginosiengineering microbial inoculum;
Grouts 2-20 part;
Humic acid powder 10-50 part;
Zeolite powder 5-30 part;
Shale 0-20 part;
Ground phosphate rock 0-10 part;
Agricultural rare-earth 0-30 part.
Wherein, used grouts, humic acid powder, zeolite powder, shale, ground phosphate rock, agricultural rare-earth all well known to a person skilled in the art, all can commercially availablely obtain.
Functional bacterial manure provided by the invention, contain the component of following weight part:
Mixture 0.2-20 part of streptomyces fradiae CGMCC No.5133 microbial inoculum and bacillusmusilaginosiengineering microbial inoculum;
Fertilizer 30-100 part;
Humic acid powder 10-50 part;
N-p-k element 0-50 part.
Wherein, fertilizer, humic acid powder, nitrogen phosphorus potassium all are well known in the art, can commercially availablely obtain.
N-p-k element is meant the NPK raw material commonly used that is used to can be used for agricultural, and the nitrogen element can be selected from urea, ammonium sulfate, ammonium chloride or its combination; Phosphorus can be selected from monoammonium phosphate, diammonium phosphate, superphosphate of lime, potassium primary phosphate, ground phosphate rock or its combination; Potassium is optional from vitriolate of tartar, Repone K, salt of wormwood, saltpetre or its combination.
In functional microbial inoculum provided by the present invention and functional bacterial manure; Streptomyces fradiae CGMCC No.5133 microbial inoculum and bacillusmusilaginosiengineering microbial inoculum are through liquid seeds liquid being inoculated into after the solid multiplication substratum carries out multiplication culture, with what prepare after the solid multiplication substratum drying.
Wherein, Can be respectively with streptomyces fradiae CGMCC No.5133 and bacillusmusilaginosiengineering carry out liquid and solid unite cultivate solid multiplication substratum drying mix after processing microbial inoculum; Also can streptomyces fradiae CGMCC No.5133 and bacillusmusilaginosiengineering be carried out liquid culture respectively; Obtain seed liquor, again seed liquor is mixed the back function and be inoculated in after the solid multiplication substratum carries out co-cultivation, the dry preparation of the solid medium after the propagation.Preferably carry out liquid culture respectively, carry out solid culture, once cultivate the viable count that the total viable count that obtains is higher than single culture, help enhancing productivity, save production cost in function.
Wherein, streptomyces fradiae CGMCC No.5133 viable count is 10～18,000,000,000/gram in the mixture of streptomyces fradiae CGMCC No.5133 microbial inoculum and the former bacteria agent of bacillusmusilaginosiengineering, and the bacillusmusilaginosiengineering viable count is 10～30,000,000,000/gram.
The functional bacterial manure of the present invention can directly be used to mix seedbed fertilizer and organic fertilizer uses; Warm Secondary Fermentation ageing processing or as fertilizer sources synergistic agent added during functional microbial inoculum can directly use, as the Secondary Fermentation bacterial classification fertilizer carried out when use other fertilizer, made the fertilizer efficiency of fertilizer increase substantially.
The present invention processes microbial inoculum with streptomyces fradiae and bacillusmusilaginosiengineering through the liquid-solid combined ferment, and processes microbial inoculum and fertilizer is used according to prescription.The present invention through cooperating bacillusmusilaginosiengineering, obtaining good potassium decomposing performance, thereby forms the bacterial classification collocation of science on the good disease-resistant of streptomyces fradiae and the basis that metabolism product such as growth hormone and fixed nitrogen phosphorus decomposing ability are provided.What is worth mentioning is that the initiative two kinds of bacterium of having explored of the present invention possess similar culture environment condition and nutritional condition, and antagonism not mutually between this two strains bacterial strain, and is collaborative each other.Can carry out multiplication by culture respectively, also can behind the zymocyte liquid that obtains separately, mix and carry out solid culture propagation.The present invention has significantly improved production efficiency of products.
Embodiment
Following examples are used to explain the present invention, but are not used for limiting scope of the present invention.
Do not specify that " part " among the application is meant " weight part " if having.
The preparation of the former bacteria agent of embodiment 1 streptomyces fradiae CLIMAX-VSF
The present invention adopts liquid-solid combined ferment culture process to carry out the cultivation of streptomyces fradiae CLIMAX-VSF, is linked into the solid culture multiplication culture again through first liquid multiplication culture, and concrete steps comprise:
1) seed preparation and liquid multiplication culture: the freeze-drying pipe that will preserve streptomyces fradiae CLIMAX-VSF earlier carries out activation.Concrete grammar is to be linked into earlier in the inclined-plane of Gause I substratum to cultivate 15 days at 30 ± 2 ℃; Behind the bacterium colony that secures good health; It is inserted in the PDA substratum 30 ℃ cultivated 5 days; Select then grow healthy bacterial classification be linked into through sterilization by in 20 parts of sucrose, 5 parts in sodium-chlor, 20 parts of peptones, 5 parts in sal epsom, 3 parts of potassium primary phosphates, 1000 parts of liquid nutrient mediums of forming of water; And then 150r/min, 30 ± 2 ℃ cultivated 3 days, obtains the streptomyces fradiae seed liquor.
(2) solid multiplication is cultivated: the streptomyces fradiae seed liquor is linked into by in 60 parts in wheat bran, 5 parts in brown sugar, 30 parts of cottonseed cakes, 1.5 parts in sal epsom, 1.5 parts in sodium-chlor, 2 parts of solid culture mediums (pH7.5) through high-temperature sterilization more than 120 ℃ of forming of lime carbonate, under 30 ± 2 ℃ aseptic aerobic conditions, cultivated 7 days.
(3) drying treatment: after solid multiplication is cultivated and finished it is put in kiln and carries out drying.Dried streptomyces fradiae detects living bacteria count through colony counting method, is 8,000,000,000/gram, preserves as the former bacteria preparation packing of streptomyces fradiae warehouse-in.
The preparation of embodiment 2 bacillusmusilaginosiengineering ACCC 10013 former bacteria agents
1) seed preparation and liquid multiplication culture: the freeze-drying pipe that will preserve bacillusmusilaginosiengineering earlier carries out activation, and concrete grammar is on the aseptic technique platform, the bacterial classification in the freeze-drying pipe to be linked in the inclined-plane of PDA substratum earlier to cultivate 5 days at 30 ± 2 ℃; Behind the bacterium colony that secures good health; It is inserted by in 5 parts of sucrose, 2 parts of Sodium phosphate, dibasics, 0.5 part of MAGNESIUM SULPHATE HEPTAHYDRATE 99.5,0.1 part in lime carbonate, 0.005 part of iron trichloride, 18 parts in agar, 1000 parts of substratum of forming of water; Cultivated 5 days for 30 ± 2 ℃; Select then grow healthy bacterial classification be linked into through sterilization by in 10 parts of murphy juices, 5 parts in sodium-chlor, 10 parts of Carnis Bovis seu Bubali creams, 5 parts in sal epsom, 0.5 part of potassium primary phosphate, 0.01 part in ferrous sulfate, 1000 parts of liquid nutrient mediums of forming of water; And cultivated 3 days 150r/min, 30 ± 2 ℃, obtain bacillusmusilaginosiengineering bacterium liquid.
2) solid multiplication is cultivated: bacillusmusilaginosiengineering bacterium liquid is linked into by in 60 parts in wheat bran, 5 parts in brown sugar, 30 parts of cottonseed cakes, 1.5 parts in sal epsom, 1.5 parts in sodium-chlor, 2 parts of solid culture mediums (pH7.5) through the above high-temperature sterilization of 120 degree of forming of lime carbonate, under 30 ± 2 ℃ aseptic aerobic conditions, cultivated 7 days.
(3) drying treatment: after solid multiplication is cultivated and finished it is put in kiln and carries out drying.Dried microbial inoculum detects living bacteria count through colony counting method, is 10,000,000,000/gram, preserves as the former bacteria preparation packing of bacillusmusilaginosiengineering warehouse-in.
The preparation of embodiment 3 functional microbial inoculums
The former bacteria agent of streptomyces fradiae CLIMAX-VSF of embodiment 1 preparation and the bacillusmusilaginosiengineering ACCC 10013 former bacteria agents of embodiment 2 preparations were mixed by weight 1: 1, be prepared into mix bacterium agent.Press the functional microbial inoculum of following formulation:
70 parts of mix bacterium agents;
2 parts of grouts;
50 parts of humic acid powder;
5 parts of zeolite powders;
20 parts in shale;
30 parts of agricultural rare-earths.
The said components mixing is promptly got microbial inoculum of the present invention.
The preparation of embodiment 4 functional microbial inoculums
Prepare mix bacterium agent by embodiment 3 identical methods.Press the functional microbial inoculum of following formulation:
5 parts of mix bacterium agents;
20 parts of grouts;
10 parts of humic acid powder;
30 parts of zeolite powders;
10 parts in ground phosphate rock.
The preparation of embodiment 5 functional microbial inoculums
Prepare mix bacterium agent by embodiment 3 identical methods.Press the functional microbial inoculum of following formulation:
50 parts of mix bacterium agents;
10 parts of grouts;
30 parts of humic acid powder;
15 parts of zeolite powders;
10 parts in shale;
10 parts in ground phosphate rock;
1 part of agricultural rare-earth.
The preparation of embodiment 6 functional microbial inoculums
The seed liquor for preparing streptomyces fradiae CGMCC No.5133 by the method for embodiment 1; The seed liquor for preparing bacillusmusilaginosiengineering ACCC10013 by the method for embodiment 2.The seed liquor of the two was mixed in 1: 1 by volume; Be inoculated in like embodiment 1 or 2 described solid multiplication substratum; And carried out multiplication culture 7 days by same procedure; Promptly get the mix bacterium agent of streptomyces fradiae CGMCC No.5133 and bacillusmusilaginosiengineering ACCC10013 after the drying, wherein measure the viable count result and be: streptomyces fradiae 6,200,000,000/gram, bacillusmusilaginosiengineering 4,600,000,000/gram.
The mix bacterium agent of above-mentioned preparation is prepared the functional microbial inoculum like embodiment 5 said prescriptions.
The preparation of embodiment 7 functional bacterial manure
The former bacteria agent of streptomyces fradiae CLIMAX-VSF of embodiment 1 preparation and the bacillusmusilaginosiengineering ACCC 10013 former bacteria agents of embodiment 2 preparations were mixed by weight 1: 1, be prepared into mix bacterium agent.Press the functional bacterial manure of following formulation:
10 parts of mix bacterium agents;
50 parts of fertilizers;
30 parts of humic acid powder;
Urea by 10 parts in nitrogen, monoammonium phosphate by 10 parts in phosphorus, Repone K by 10 parts in potassium.
The said components mixing is promptly got bacterial manure of the present invention.
The preparation of embodiment 8 functional bacterial manure
Prepare mix bacterium agent by embodiment 7 identical methods.Press the functional bacterial manure of following formulation:
20 parts of mix bacterium agents;
30 parts of fertilizers;
50 parts of humic acid powder.
The said components mixing is promptly got bacterial manure of the present invention.
The preparation of embodiment 9 functional bacterial manure
Prepare mix bacterium agent by embodiment 7 identical methods.Press the functional bacterial manure of following formulation:
0.2 part of mix bacterium agent;
100 parts of fertilizers;
10 parts of humic acid powder.
Ammonium chloride by 20 parts in nitrogen, superphosphate of lime by 20 parts in phosphorus, vitriolate of tartar by 10 parts in potassium.
The said components mixing is promptly got bacterial manure of the present invention.
Test Example 1 functional microbial inoculum
The functional microbial inoculum of embodiment 3～5 is used as seed manure on the tobacco seedbed, and 200 grams are used in every square metre of seedbed.Do not contain streptomyces fradiae CGMCC No.5133 microbial inoculum and compare with using with other moity of bacillusmusilaginosiengineering ACCC 10013 microbial inoculums; Can make tobacco date of emerging shift to an earlier date 1-2 days after using, the unit surface digital display work of emerging increases the fibrous root showed increased; It is vigorous to show growth; Later stage is followed the trail of its single leaf mass ratio contrast significantly to be increased, and in addition, indexs such as plant height, stem girth, blade length and width are all compared according to significantly increasing (table 1).
The effect of the functional microbial inoculum of table 1 the present invention
Test Example 2 functional bacterial manure
The functional bacterial manure of embodiment 7～9 is used as the seedbed is fertile on the tobacco seedbed, and every square metre is used 200 grams.Do not contain streptomyces fradiae CGMCC No.5133 microbial inoculum and compare with using with other moity of bacillusmusilaginosiengineering ACCC 10013 microbial inoculums; Later stage follows the tracks of and to show that better-than-average cigarette compares respectively according to increasing by 10.2%, 11.7%, 13.0%; And effectively prevent tobacco mosaic disease, make disease reduce more than 30%.
Through Test Example 1 and 2; Show that bacterial classification compatibility of the present invention is a science; On the basis of having given play to the disease-resistant of streptomyces fradiae and promotion growth and significant fixed nitrogen phosphate solubilization, the activity of bacillusmusilaginosiengineering in soil further improved the supply of potassium element to plant.Finally show as the excellent properties that improves output, raising quality, reduces disease three aspects.
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from know-why of the present invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (10)
1. the functional microbial inoculum that contains streptomyces fradiae CGMCC No.5133 and bacillusmusilaginosiengineering.
2. microbial inoculum according to claim 1 is characterized in that, contains the component of following weight part:
Mixture 5-70 part of streptomyces fradiae CGMCC No.5133 microbial inoculum and bacillusmusilaginosiengineering microbial inoculum;
Grouts 2-20 part;
Humic acid powder 10-50 part;
Zeolite powder 5-30 part;
Shale 0-20 part;
Ground phosphate rock 0-10 part;
Agricultural rare-earth 0-30 part.
3. microbial inoculum according to claim 2; It is characterized in that; Streptomyces fradiae CGMCCNo.5133 microbial inoculum and bacillusmusilaginosiengineering microbial inoculum are through liquid seeds liquid being inoculated into after the solid multiplication substratum carries out multiplication culture, with what prepare after the solid multiplication substratum drying.
4. according to each described microbial inoculum of claim 1～3, it is characterized in that described bacillusmusilaginosiengineering is bacillusmusilaginosiengineering ACCC10013.
5. according to each described microbial inoculum of claim 1～3; It is characterized in that; Streptomyces fradiae CGMCC No.5133 viable count is 10-180 hundred million/gram in the mixture of streptomyces fradiae CGMCC No.5133 microbial inoculum and the former bacteria agent of bacillusmusilaginosiengineering, and the bacillusmusilaginosiengineering viable count is 10～30,000,000,000/gram.
6. the functional bacterial manure that contains streptomyces fradiae CGMCC No.5133 and bacillusmusilaginosiengineering.
7. bacterial manure according to claim 6 is characterized in that, contains the component of following weight part:
Mixture 0.2-20 part of streptomyces fradiae CGMCC No.5133 microbial inoculum and bacillusmusilaginosiengineering microbial inoculum;
Fertilizer 30-100 part;
Humic acid powder 10-50 part;
N-p-k element 0-50 part.
8. bacterial manure according to claim 7; It is characterized in that; Streptomyces fradiae CGMCCNo.5133 microbial inoculum and bacillusmusilaginosiengineering microbial inoculum are through liquid seeds liquid being inoculated into after the solid multiplication substratum carries out multiplication culture, with what prepare after the solid multiplication substratum drying.
9. according to each described bacterial manure of claim 6～8, it is characterized in that described bacillusmusilaginosiengineering is bacillusmusilaginosiengineering ACCC10013.
10. according to each described bacterial manure of claim 6～8; It is characterized in that; Streptomyces fradiae CGMCC No.5133 viable count is 10～18,000,000,000/gram in the mixture of streptomyces fradiae CGMCC No.5133 microbial inoculum and the former bacteria agent of bacillusmusilaginosiengineering, and the bacillusmusilaginosiengineering viable count is 10～30,000,000,000/gram.
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Application publication date: 20120509 Assignee: BEIJING JINGGENG TIANXIA AGRICULTURAL SCIENCE AND TECHNOLOGY CO.,LTD. Assignor: BEIJING CLIMAX BIOTECHNOLOGY Co.,Ltd. Contract record no.: 2014990000085 Denomination of invention: Functional bacterial manure and preparation method thereof Granted publication date: 20130605 License type: Exclusive License Record date: 20140226 |
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Effective date of registration: 20230302 Address after: 102200 No.11, Huatong Road, science and Technology Park, Changping District, Beijing Patentee after: BEIJING JINGGENG TIANXIA AGRICULTURAL SCIENCE AND TECHNOLOGY CO.,LTD. Address before: No. 11, Changping Yuanhua Road, Zhongguancun Science Park, Beijing 102200 Patentee before: BEIJING CLIMAX BIOTECHNOLOGY Co.,Ltd. |