CN103641605B - High-fertility long-acting anti-verticillium-wilt biological bacterial fertilizer and preparation method thereof - Google Patents
High-fertility long-acting anti-verticillium-wilt biological bacterial fertilizer and preparation method thereof Download PDFInfo
- Publication number
- CN103641605B CN103641605B CN201310618874.9A CN201310618874A CN103641605B CN 103641605 B CN103641605 B CN 103641605B CN 201310618874 A CN201310618874 A CN 201310618874A CN 103641605 B CN103641605 B CN 103641605B
- Authority
- CN
- China
- Prior art keywords
- fertilizer
- soil
- bacterial manure
- bacterial
- microbial inoculum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 57
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 239000003337 fertilizer Substances 0.000 title abstract description 81
- 210000003608 fece Anatomy 0.000 claims abstract description 107
- 239000010871 livestock manure Substances 0.000 claims abstract description 107
- 239000002689 soil Substances 0.000 claims abstract description 91
- 238000002156 mixing Methods 0.000 claims abstract description 23
- 241000082085 Verticillium <Phyllachorales> Species 0.000 claims abstract description 21
- 241000287828 Gallus gallus Species 0.000 claims abstract description 8
- 239000002245 particle Substances 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 117
- 239000002068 microbial inoculum Substances 0.000 claims description 27
- 239000008187 granular material Substances 0.000 claims description 24
- 230000035558 fertility Effects 0.000 claims description 21
- 239000000463 material Substances 0.000 claims description 20
- 230000004913 activation Effects 0.000 claims description 17
- 241000194107 Bacillus megaterium Species 0.000 claims description 12
- 241000187419 Streptomyces rimosus Species 0.000 claims description 12
- 238000000855 fermentation Methods 0.000 claims description 12
- 230000004151 fermentation Effects 0.000 claims description 12
- 239000002994 raw material Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 230000007774 longterm Effects 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 10
- 239000011230 binding agent Substances 0.000 claims description 7
- 238000005469 granulation Methods 0.000 claims description 7
- 230000003179 granulation Effects 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 239000002609 medium Substances 0.000 claims description 5
- 238000003892 spreading Methods 0.000 claims description 5
- 230000007480 spreading Effects 0.000 claims description 5
- 244000052616 bacterial pathogen Species 0.000 claims description 4
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 239000010902 straw Substances 0.000 claims description 4
- 241000881860 Paenibacillus mucilaginosus Species 0.000 claims description 3
- 239000007633 bacillus mucilaginosus Substances 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 238000013461 design Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 238000012163 sequencing technique Methods 0.000 claims description 3
- 244000046052 Phaseolus vulgaris Species 0.000 claims 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 244000061456 Solanum tuberosum Species 0.000 claims 1
- 235000002595 Solanum tuberosum Nutrition 0.000 claims 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 1
- 229930006000 Sucrose Natural products 0.000 claims 1
- 210000000582 semen Anatomy 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 239000005720 sucrose Substances 0.000 claims 1
- 244000061458 Solanum melongena Species 0.000 abstract description 35
- 230000000694 effects Effects 0.000 abstract description 33
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 32
- 235000002597 Solanum melongena Nutrition 0.000 abstract description 30
- 239000002028 Biomass Substances 0.000 abstract description 27
- 238000012360 testing method Methods 0.000 abstract description 20
- 230000009466 transformation Effects 0.000 abstract description 20
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 19
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 17
- 240000003768 Solanum lycopersicum Species 0.000 abstract description 17
- 239000011574 phosphorus Substances 0.000 abstract description 17
- 102000004190 Enzymes Human genes 0.000 abstract description 16
- 108090000790 Enzymes Proteins 0.000 abstract description 16
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 16
- 235000007688 Lycopersicon esculentum Nutrition 0.000 abstract description 15
- 241000894006 Bacteria Species 0.000 abstract description 13
- 239000005416 organic matter Substances 0.000 abstract description 12
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 235000002568 Capsicum frutescens Nutrition 0.000 abstract description 4
- 239000003895 organic fertilizer Substances 0.000 abstract description 4
- 235000013311 vegetables Nutrition 0.000 abstract description 4
- 201000010099 disease Diseases 0.000 abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 3
- 239000003795 chemical substances by application Substances 0.000 abstract description 2
- 230000004720 fertilization Effects 0.000 abstract 5
- 150000002484 inorganic compounds Chemical class 0.000 abstract 3
- 229910010272 inorganic material Inorganic materials 0.000 abstract 3
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 230000008569 process Effects 0.000 description 114
- 240000008574 Capsicum frutescens Species 0.000 description 23
- 239000002131 composite material Substances 0.000 description 20
- 235000002566 Capsicum Nutrition 0.000 description 19
- 239000001390 capsicum minimum Substances 0.000 description 18
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 13
- 241000196324 Embryophyta Species 0.000 description 10
- 102000016938 Catalase Human genes 0.000 description 7
- 108010053835 Catalase Proteins 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 230000004083 survival effect Effects 0.000 description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 6
- 230000000813 microbial effect Effects 0.000 description 6
- 229910052700 potassium Inorganic materials 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 238000003860 storage Methods 0.000 description 5
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 206010025482 malaise Diseases 0.000 description 4
- 239000011591 potassium Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000005070 ripening Effects 0.000 description 4
- 230000003442 weekly effect Effects 0.000 description 4
- 108010013043 Acetylesterase Proteins 0.000 description 3
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 3
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000000470 constituent Substances 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- 235000021049 nutrient content Nutrition 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 238000009331 sowing Methods 0.000 description 3
- 241000726221 Gemma Species 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000036983 biotransformation Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 1
- 240000008384 Capsicum annuum var. annuum Species 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000009847 Cucumis melo var cantalupensis Nutrition 0.000 description 1
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- 241001123668 Verticillium dahliae Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000003895 groundwater pollution Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Landscapes
- Fertilizers (AREA)
Abstract
The invention relates to a high-fertility long-acting anti-verticillium-wilt biological bacterial fertilizer and a preparation method thereof. The biological bacterial fertilizer is formed by mixing bacterial fertilizer carrier grains and bacterium agent particles. Compared with that of a biological bacterial fertilizer which is commonly used in production, the fertilizer efficiency of the biological bacterial fertilizer is higher. By taking chilies, eggplants and tomatoes as fertilization objects and taking no fertilization, fertilization with an inorganic compound fertilizer and fertilization with an organic fertilizer, chicken manure, as contrasts, the biological bacterial fertilizer can be used for obviously promoting biological yields of the three vegetables, and the biomass of the three vegetables applied with the bacterial fertilizer is more than 50% higher than that of vegetables which are not applied with fertilizer, applied with the inorganic compound fertilizer and applied with the organic fertilizer, chicken manure. A test on eggplants inoculated with verticillium wilt shows that the bacterial fertilizer can be used for effectively inhibiting and relieving the diseases. A soil enzyme activity test shows that the enzyme activity of soil treated by the biological bacterial fertilizer is obviously higher than that of soil without fertilization and that of soil applied with the inorganic compound fertilizer or the organic fertilizer, chicken manure; nitrogen transformation, phosphorus transformation and organic matter transformation capabilities of soil can be facilitated well.
Description
Technical field
The present invention relates to a kind of high fertility long term effective resisting verticillium bio-bacterial manure and preparation technology thereof, especially nitrogen, phosphorus element and organic matter transformation ability is high and living bacteria count is high and can the bio-bacterial manure of resisting verticillium.
Background technology
China just starts production and the research of microbial fertilizer as far back as the fifties, and makes great progress through experiment for many years and research, in long-term agricultural production application, achieve good effect.In China's microbial fertilizer, being most widely used of rhizobia fertilizer, wherein soybean, peanut, Herba Astragali Melilotoidis (Herba Astragali Sinici) and leguminous forage vaccination area are comparatively large, and effect of increasing production is obvious.Meanwhile, other kind bacterial manure of China development in recent years rapidly.In biologic compound fertilizer research and development field, many colleges and universities and research institution with industrial community cooperation, adopt industries, universities and research institutors combine Development Finance, make the rapid industrialization of scientific payoffs, achieve obvious economic benefit.At present, Biological Compound Fertilizer manufacturing enterprise of China scale, generally at 1.5 ~ 3.5 ten thousand tons/year, has 8 kinds of bio-feritlizers to obtain Ministry of Agriculture's certification first, and promotes the use of at home.But domestic only have 250 ~ 300 enterprises now, produces 600000 tons of bacterial fertilizer per year.And chemical fertilizer annual production is 1.2 hundred million tons.The output of microbial fertilizer is only 1/200 of chemical fertilizer.Relevant expert estimates, if microbial fertilizer output is 3% of chemical fertilizer, then can produce more food 50 ~ 1,000,000 tons/year.And a large amount of uses of chemical fertilizer also can cause groundwater pollution problem day by day serious, the microbial fertilizer of Development and Production high-efficiency high-quality is the eager needs developed eco-agriculture, and meets the requirement of the strategy of sustainable development.Visible, microbial fertilizer is the agricultural prods urgently developed in China, and has bright application prospect.
One in bio-bacterial manure very important index is exactly high fertilizer efficiency, key of increasing soil fertility improves the activity of soil enzyme, soil enzyme plays very important effect in the material cycle and Conversion of energy of soil ecosystem, all living things chemical reaction in its catalysis soil, reflect intensity and the direction of various Biochemical processes in soil, its active size is the important symbol of soil fertility.Crucial soil enzyme mainly contains urase, Phosphoric acid esterase, catalase.Wherein urase is a kind of lytic enzyme acting on the C-N key (non-skin) of phthalein amine, is the index evaluating soil nitrogen bio-transformation direction and intensity, and its active power has substantial connection with nitrogen transformation speed.Phosphatase activity is the index evaluating soil phosphorus bio-transformation direction and intensity, and its active size and soil available phosphorus number are proportionate.Catalase is the important indicator evaluating soil fertility organic matter transformation ability, by the analysis of enzymic activity, can indirectly understand or predict conversion situation and the soil fertility of some nutritive substance.In soil in organic substance conversion process, catalase occupies an important position.
The fertilizer efficiency of bio-bacterial manure is relevant with living bacteria count in bacterial manure, and the fertilizer efficiency of a lot of bio-bacterial manure can because long storage periods, and the viable count of bio-bacterial manure reduces and is affected.The bio-bacterial manure of how to prepare high storage period seems particularly important.
Plant verticillium wilt endangers comparatively serious vascular bundle diseases, mainly infect tomato, eggplant, green pepper, muskmelon, Chinese cabbage, radish, soya bean etc., host is very extensive, belongs to more typical soil-borne disease, causes a large amount of losses every year to cash crop such as vegetable and fruits.Use chemicals process to there is the risk of pesticide residue, self improve Soil Probiotics environment if can be utilized by bio-bacterial manure, suppress or kill pathogenic bacteria, will important biological control means be become.
Summary of the invention
In order to solve above-mentioned the deficiencies in the prior art, primary and foremost purpose of the present invention is that providing a kind of strengthens soil enzyme activities, improve transformation of soil nitrogen, phosphorus element transform and organic matter transformation ability, there is high storage period simultaneously, and can resisting verticillium bio-bacterial manure formula and preparation method thereof.
Object of the present invention is realized by following proposal:
A preparation method for high fertility long term effective resisting verticillium bio-bacterial manure is obtained than compound according to the quality of 90:1 bacterial manure carrier granule and microbial inoculum particulate;
Raw materials and the proportioning thereof of described bacterial manure carrier granule are:
50wt% chicken manure
20wt% colliery powder
15wt% crushing straw
15wt% wilkinite
The raw materials of above-mentioned bacterial manure carrier granule is ground into one by one 20 object fine powder batchings, to add in stirrer by above-mentioned design proportioning and sequencing and add binding agent and stir, the fully mixing that adds water makes water content lower than 25wt%, extruder grain, dries 15min to kill pathogenic bacterium for 80 DEG C; Namely bacterial manure carrier granule is obtained after cooling;
Described microbial inoculum particulate is by first actication of culture, spreads cultivation, the step of rear mixing granulation and obtaining, specific as follows:
(1) activation of bacterial classification: get bacillus megaterium J1(Bacillus megatherium respectively by inclined-plane), bacillusmusilaginosiengineering J2 (Bacillus mucilaginosus) and streptomyces rimosus J3(Streptomyces rimosus) respective access liquid activation medium in activate, thus obtain the culture material after three kinds of activation;
Activation culture based formulas, to add 1000ml H
2the substratum meter of O is specific as follows:
(2) the spreading cultivation of bacterial classification: the culture material after above-mentioned three kinds of activation is mixed according to the mass ratio of 1:2 with the raw materials (mixture namely obtained by this quality proportioning: 50wt% chicken manure, 20wt% colliery powder, 15wt% crushing straw and 15wt% wilkinite) of mixed bacterial manure carrier granule respectively, at 18 ~ 30 DEG C, solid fermentation 3-5 days, obtains fermentation material; Be bacterial classification again with fermentation material, inoculate the raw materials of mixed bacterial manure carrier granule, mix according to the mass ratio of 1:10, solid fermentation 15 days at 18 ~ 30 DEG C, finally makes viable count wherein all reach 10
12above, and bacillus megaterium J1 and bacillusmusilaginosiengineering J2 is cultured to the gemma ripening stage, and streptomyces rimosus J3 is cultured to the spore ripening stage; Thus obtain the microbial inoculum of three kinds of fermenting-ripenings;
Bacterial classification activates separately, spread cultivation, and is conducive to flash baking and preserves, can significantly improve viable count.
(3) mixing granulation: the streptomyces rimosus J3 microbial inoculum of the bacillus megaterium J1 microbial inoculum of fermenting-ripening step (2) obtained, the bacillusmusilaginosiengineering J2 microbial inoculum of fermenting-ripening and fermenting-ripening is according to after volume ratio 1:2:2 mixing, add water after it being mixed with binding agent stirring, controlling water content makes it lower than 25wt%, extruder grain becomes the particulate of particle diameter≤1mm, cool after 75 DEG C of oven dry 15min, obtain microbial inoculum particulate.
The oven dry of above-mentioned bacterial manure carrier granule and microbial inoculum particulate is following current dries, specifically wet granular is entered in dram by the intermediate bin of dryer front portion, meanwhile hot blast enters dram in a co-current manner, under in rotary barrel, flight drives, material is taken up, and forms point material curtain spreading uniform comparatively dense, thus adds the contact of material and warm air, not only increase heat transfer coefficient, and it also avoid the short circuit of hot gas flow.
Described dryer can select any one can complete the commercially available dryer of baking step, but the dryer of this model of preferred following manufacturer production: Zhangqiu Yulong Machine Co., Ltd., following current roller dryer GHG ф 1.0*12*1.
The present invention's claimed high fertility long term effective resisting verticillium bio-bacterial manure obtained by the preparation method of above-mentioned high fertility long term effective resisting verticillium bio-bacterial manure simultaneously.
Beneficial effect: after testing, in high fertility long term effective resisting verticillium provided by the invention bio-bacterial manure, live body bacterium number is more than 10
12cfu/g, moisture content is lower than 10%.Use the strengthened soil enzyme activities of this fertilizer, improve transformation of soil nitrogen, phosphorus element transforms and organic matter transformation ability, there is high storage period simultaneously, and the outstanding advantages of energy resisting verticillium.Owing to have employed the mode of bacterial manure carrier granule and microbial inoculum particulate compound, carrier granule can make the bacterium number in live bacteria agent particulate breed, and the fertility slowly-releasing of this bacterial manure, fertilizer efficiency period are increased.
Accompanying drawing explanation
Fig. 1 is bacterial manure carrier granule form prepared by the inventive method;
This bacterial manure carrier granule appearance beige, cylindrical, pH7.2, moisture content is lower than 8%, and organic content is higher than 55%.
Fig. 2 is microbial inoculum particulate form prepared by the inventive method;
This microbial inoculum particulate is spherical, diameter 1-2mm, and brown, living bacteria count is more than 10
11cfu/g.
Fig. 3 is the capsicum growing way figure through different modes process in test example 1;
Four kinds of process experimental results show, bio-bacterial manure process soil capsicum growing way of the present invention is the most obvious, and no matter biomass, output or nitrogen, phosphorus organic matter transformation enzymic activity and fertilizer efficiency period are all apparently higher than other three kinds process.
Fig. 4 be in test example 2 after different modes process eggplant growing way figure;
Four kinds of process experimental results show, bio-bacterial manure process soil capsicum growing way of the present invention is the most obvious, and no matter biomass or nitrogen, phosphorus and organic matter transformation enzymic activity are all apparently higher than other three kinds process.Meanwhile, resistance to blight ability is also apparently higher than other three kinds process.
Fig. 5 be in test example 3 after different modes process tomato growing way figure;
Four kinds of process experimental results show, bio-bacterial manure process soil capsicum growing way of the present invention is the most obvious, and no matter biomass or nitrogen, phosphorus and organic matter transformation enzymic activity are all apparently higher than other three kinds process.
In figs. 3-5,1-4 are respectively: 1. bio-bacterial manure process soil of the present invention, 2. fertilizer-chicken manure process soil, 3. inorganic composite fertilizer process soil, 4. soil fertility quality soil
Embodiment
Below in conjunction with enforcement illustration, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment
Bacterial manure carrier granule and microbial inoculum particulate are obtained the bio-bacterial manure of high fertility long term effective resisting verticillium according to the quality of 90:1 than compound;
Raw materials and the proportioning thereof of described bacterial manure carrier granule are:
50wt% chicken manure
20wt% colliery powder
15wt% crushing straw
15wt% wilkinite
The raw materials of above-mentioned bacterial manure carrier granule is ground into one by one 20 object fine powder batchings, to add in stirrer in above-mentioned component design ratio and sequencing and add binding agent and stir, the fully mixing that adds water makes water content lower than 25wt%, extruder grain, dries 15min to kill pathogenic bacterium for 80 DEG C; Namely bacterial manure carrier granule is obtained after cooling; Described content of binder is also unrestricted, with can smoothly granulation for standard.
Described microbial inoculum particulate is by first actication of culture, spreads cultivation, the step of rear mixing granulation and obtaining, specific as follows:
(1) activation of bacterial classification: get bacillus megaterium J1(Bacillus megatherium respectively by inclined-plane), bacillusmusilaginosiengineering J2 (Bacillus mucilaginosus) and streptomyces rimosus J3(Streptomyces rimosus) respective access liquid activation medium in activate, thus obtain the culture material after three kinds of activation;
Activation culture basigamy, to add 1000ml H
2the substratum meter of O is specific as follows:
(2) the spreading cultivation of bacterial classification: mixed according to the mass ratio of 1:2 with the raw materials of mixed bacterial manure carrier granule respectively by the culture material after above-mentioned three kinds of activation, at 18 ~ 25 DEG C, solid fermentation 3-5 days, obtains fermentation material; Be bacterial classification again with fermentation material, inoculate the raw materials of mixed bacterial manure carrier granule, mix according to the mass ratio of 1:10, solid fermentation 15 days at 18 ~ 25 DEG C, finally makes viable count wherein all reach 10
11above, and bacillus megaterium J1 and bacillusmusilaginosiengineering J2 is cultured to the gemma ripening stage, and streptomyces rimosus J3 is cultured to the spore ripening stage; Thus obtain the microbial inoculum of three kinds of fermenting-ripenings;
Adopt bacterial classification to activate separately, spread cultivation, be conducive to flash baking and preserve, can viable count be significantly improved.
(3) mixing granulation: the streptomyces rimosus J3 microbial inoculum of the bacillus megaterium J1 microbial inoculum of fermenting-ripening step (2) obtained, the bacillusmusilaginosiengineering J2 microbial inoculum of fermenting-ripening and fermenting-ripening is according to after volume ratio 1:2:2 mixing, add water after it is mixed with binding agent and be stirred to water content lower than 25wt%, extruder grain becomes the particulate of particle diameter≤1mm, cool after 75 DEG C of oven dry 15min, obtain microbial inoculum particulate.
Preferably, above-mentioned oven dry is following current and dries, specifically wet granular is entered in dram by the intermediate bin of dryer front portion, meanwhile hot blast enters dram in a co-current manner, and under in rotary barrel, flight drives, material is taken up, form point material curtain spreading uniform comparatively dense, thus add the contact of material and warm air, not only increase heat transfer coefficient, and it also avoid the short circuit of hot gas flow.
Described dryer can select any one can complete the commercially available dryer of baking step, but the dryer of this model of preferred following manufacturer production: Zhangqiu Yulong Machine Co., Ltd., following current roller dryer GHG ф 1.0*12*1.
To sieve hierarchical verification and packaging
Through vibratory screening apparatus classification, excessive and mistake small-particle are returned pulverizer, after pulverizing, mixes new material recycling.Then respectively by the carrier granule for preparing and microbial inoculum particulate according to 90:1 Homogeneous phase mixing.Sampling Detection, in bacterial manure, live body bacterium number is more than 10
12cfu/g, moisture content is lower than 10%.Quantitatively pack after the assay was approved, the double braid bag of inner membrance is preferentially selected in packing bag, is conducive to keeping moisture and preventing bacterial manure to be subject to outside contamination.
Test example 1:
The bio-bacterial manure utilizing the present invention to prepare is to the manure trial of eggplant biomass and anti-eggplant verticillium wilt thereof:
(1) test site is located in Dalian Nationality College greenhouse and carries out, and soil is normal loam, and Eggplant Varieties of participating in the experiment is distant eggplant No. 7.First use fine earth vernalization, when seedling grew to for 2 leaf phase, survival after transplant is in flowerpot respectively, each process 10 basin, every basin 2 eggplants, 4 processing modes, and 2 groups of experiments (being respectively used to biomass measuring and inoculation eggplant verticillium wilt resistant proof) add up to 160 eggplants.
(2) 4 process are established in test:
1. bio-bacterial manure process soil (hereinafter referred to as process 1) of the present invention
2. fertilizer-chicken manure process soil (hereinafter referred to as process 2)
3. inorganic composite fertilizer process soil (hereinafter referred to as process 3), described inorganic composite fertilizer is the prosperous board inorganic composite fertilizer of fringe that Shandong Henkel biotechnology company limited produces, model: N-P2O5-K2O15-12-18. active constituent content: N P and K.Total nutrient content >=: 45(%), available phosphorus content >=: 12(%), moisture content≤: 3(%), effective potassium content >=: 18(%), total nitrogen content >=: 15(%).
4. without soil fertility quality soil (hereinafter referred to as process 4)
(3) fertilizer applies mode
Inorganic composite fertilizer (process 3) process of topdressing, in transplanting after 2 weeks, eggplant seedling restore normal growth, and starting to apply inorganic composite fertilizer sowing amount is 20g/ basin, dig two seedlings centres spread manuer in holes into.
Fertilizer-chicken manure (process 2) and bio-bacterial manure of the present invention (process 1) all apply as seed manure, namely when transplanting eggplant seedling, respectively by fertilizer-chicken manure and bio-bacterial manure of the present invention with transplant mixing with soil, fertilizer-chicken manure and mixing with soil ratio are 1:3, biological bacteria of the present invention and mixing with soil ratio are 1:7, then load flowerpot, transplant eggplant seedling.
(4) manage
After transplanting, under 4 kinds of process are placed in same environment, normal management, no longer enriches any fertilizer, observes eggplant growing way weekly.
(5) test, testing data statistics and result
Biomass measuring and resistance to verticillium wilt test:
Grew into for 6 leaf phases until the eggplant through various process, carefully extract eggplant, remove eggplant root soil, measure eggplant plant height and dry weight.Latter 2 weeks of eggplant fertilising, inoculation Verticillium Wilt Pathogen of Eggplant (Verticillium dahliae), during inoculation, scratches soil with cutter, causes eggplant root system to injure, according to every basin 50ml(spore concentration more than 10 in the middle of 2 young plants
6individual/ml) inoculum size, four kinds of process are identical, a month " Invest, Then Investigate " sickness rate, and whole vegetative period observes Strain survival situation, investigation survival rate.After different modes process, eggplant growing way is shown in Fig. 3.
From table 1 experimental result, the soil eggplant growing way of bio-bacterial manure process of the present invention is vigorous, the speed of growth is obviously faster than other 3 kinds process, no matter be eggplant plant height or biomass are all more than 3 times of non-fertilizer treatment, be have a surplus without 2 times of base composite fertilizer process, be also significantly better than fertilizer-chicken manure process simultaneously.Eggplant leaf thickness is all better than other 3 kinds process simultaneously, and color is also darker compared with other 3 kinds process.The experimental result of inoculation verticillium wilt pathogen shows, although the sickness rate inoculating rear 4 kinds of process is all higher, process 1 is also up to 75%, and process 1 morbidity is more late.And observe through whole vegetative period, through the soil of bacterial manure process, although process 1 sickness rate is also higher, but final survival rate is to 75%, significantly higher than other 3 kinds process, and start normal outcome, although and other 3 kinds process also have part to survive, but there is no a strain result, illustrate that the soil through bio-bacterial manure process of the present invention has resisting verticillium and repairing effect.
The process of table 1 different fertilizer is to eggplant biomass and resistance assay result
| Process | 1 | 2 | 3 | 4 |
| Eggplant plant height (cm) | 21.4 | 16.9 | 10.5 | 7.3 |
| Eggplant biomass (weight in wet base, g) | 7.341 | 5.223 | 3.470 | 2.146 |
| Sickness rate | 75% | 85% | 80% | 100% |
| Survival rate | 75% | 45% | 35% | 5% |
Different fertilizer process is on the impact of soil enzyme activities: when the eggplant 6 leaf phase, gets each process flowerpot soil respectively.Fetch earth earth time, each potted plant is carefully pulled out (weigh biomass with), fully mixed by flowerpot soil, every basin fetches earth 200g, puts into plastics bag and takes back laboratory, each soil enzyme activities of rapid test.
From the test-results of table 2,3 kinds of enzymic activitys of process 4 are all apparently higher than other three kinds process, especially phosphatase activity and catalase activity are 2 times of the corresponding enzymic activity of no-fertilization, illustrate that its transformation of soil nitrogen of soil of bacterial manure process, phosphorus element transforms and organic matter transformation ability processes apparently higher than other, especially phosphorus element and organic matter transformation ability are significantly better than inorganic fertilizer and fertilizer.
The process of table 2 different fertilizer is on the impact (μ g/g) of eggplant root soil enzyme activities
Test example 2
The bio-bacterial manure utilizing the present invention to prepare is on the impact of capsicum biomass and output thereof:
(1) be experimental field located in Dalian Nationality College greenhouse and carry out, soil is normal loam, and capsicum variety of participating in the experiment is redly select No. 2.First use fine earth vernalization, when seedling grew to for 2 leaf phase, survival after transplant is in flowerpot respectively, each process 10 basin, every basin 2 capsicums, and 4 process are used for biomass measuring and yield monitoring) add up to 40 eggplants.
(2) 4 process are established in test:
1. bio-bacterial manure process soil (hereinafter referred to as process 1) of the present invention
2. fertilizer-chicken manure process soil (hereinafter referred to as process 2)
3. inorganic composite fertilizer process soil (hereinafter referred to as process 3), described inorganic composite fertilizer is the prosperous board inorganic composite fertilizer of fringe that Shandong Henkel biotechnology company limited produces, model: N-P2O5-K2O15-12-18. active constituent content: nitrogen, phosphorus, potassium.Total nutrient content >=: 45(%), available phosphorus content >=: 12(%), moisture content≤: 3(%), effective potassium content >=: 18(%), total nitrogen content >=: 15(%).4. without soil fertility quality soil (hereinafter referred to as process 4)
(3) fertilizer applies mode
Inorganic composite fertilizer (process 3) process of topdressing, in transplanting after 2 weeks, Hot Pepper Seedling restore normal growth, starts to apply inorganic composite fertilizer, and sowing amount is 20g/ basin, dig two seedlings centres spread manuer in holes into.
Fertilizer-chicken manure (process 2) and bio-bacterial manure of the present invention (process 1) all apply as seed manure, namely when transplanting Hot Pepper Seedling, respectively by fertilizer-chicken manure and bio-bacterial manure of the present invention with transplant mixing with soil, fertilizer-chicken manure and mixing with soil ratio are 1:3, biological bacteria of the present invention and mixing with soil ratio are 1:7, then load flowerpot, transplant Hot Pepper Seedling.
(4) manage
After transplanting, under 4 kinds of process are placed in same environment, normal management, no longer enriches any fertilizer, observes capsicum growing way weekly.
(5) investigation and result
Biomass measuring: when capsicum grows to 6 leaf, measure capsicum plant height and biomass, during whole chili growth season, capsicum reddens and gathers at any time, dries, and after whole Growing season terminates, measures yield of hot pepper.After different modes process, capsicum growing way is shown in Fig. 4.
From table 3 experimental result, the soil capsicum growing way of bio-bacterial manure process of the present invention is vigorous, the speed of growth is obviously faster than other 3 kinds process, nearly 3 times of non-fertilizer treatment that no matter to be eggplant plant height or biomass be all, be nearly 2 times without base composite fertilizer process, be also significantly better than fertilizer-chicken manure process simultaneously.Eggplant leaf thickness is all better than other 3 kinds process simultaneously, and color is also darker compared with other 3 kinds process.Determination of yield result shows, through the soil yield of hot pepper of bio-bacterial manure process of the present invention apparently higher than other 3 kinds process, the average single plant yield dry weight of bacterial manure process soil capsicum reaches 36.71g, is nearly 3 times of fertilizer chicken manure process, more than 9 times of inorganic fertilizer composite fertilizer process.And the capsicum of not executing any fertilizer soil does not almost produce fruit.
The process of table 3 different fertilizer is on the impact of capsicum plant height, Biomass and yield
| Process | 1 | 2 | 3 | 4 |
| Plant height (cm) | 19.4 | 17.2 | 8.6 | 5.7 |
| Biomass (weight in wet base, g) | 6.319 | 5.483 | 2.315 | 1.746 |
| Single plant yield (dry weight, g) | 36.71 | 12.32 | 3.94 | 0 |
| Single plant yield (weight in wet base, g) | 444.36 | 149.47 | 42.37 | 0 |
Different fertilizer process is on the impact of soil enzyme activities: when the capsicum 6 leaf phase, gets each process flowerpot soil respectively.Fetch earth earth time, each potted plant is carefully pulled out (weigh biomass with), fully mixed by flowerpot soil, every basin fetches earth 200g, puts into plastics bag and takes back laboratory, each soil enzyme activities of rapid test.
From the test-results of table 4,3 kinds of enzymes of process 1 and urase, Phosphoric acid esterase and catalase activity are all apparently higher than other three kinds process, the soil of bacterial manure process is described, its transformation of soil nitrogen, phosphorus element transforms and organic matter transformation ability processes apparently higher than other.
The process of table 4 different fertilizer is on the impact (μ g/g) of capsicum root soil enzyme activities
Fertility slowly-releasing, the fertilizer efficiency period of bacterial manure increase:
After transplanting, under 4 kinds of process are placed in same environment, normal management, whole Growing season no longer enriches any fertilizer simultaneously, observes capsicum growing way weekly, until whole Growing season terminates.Result shows, until whole Growing season terminates, the capsicum growing way of bacterial manure process is always best, is that biomass or output are all the highest, and whole advantage is retained to Growing season and terminates, and illustrates that this bacterial manure has fertility slowly-releasing, effect that fertilizer efficiency period is long.
Test example 3
The bio-bacterial manure utilizing the present invention to prepare is on the impact of tomato biological amount:
(1) be experimental field located in Dalian Nationality College greenhouse and carry out, soil is normal loam, and tomato variety of participating in the experiment is L402.First use fine earth vernalization, when seedling grew to for 2 leaf phase, survival after transplant is in flowerpot respectively, each process 10 basin, every basin 2 tomatoes, and 4 process are used for biomass measuring and yield monitoring) add up to 40 tomatoes.
(2) 4 process are established in test:
1. bio-bacterial manure process soil (hereinafter referred to as process 1) of the present invention;
2. fertilizer-chicken manure process soil (hereinafter referred to as process 2);
3. inorganic composite fertilizer process soil (hereinafter referred to as process 3), described inorganic composite fertilizer is the prosperous board inorganic composite fertilizer of fringe that Shandong Henkel biotechnology company limited produces, model: N-P2O5-K2O15-12-18. active constituent content: N P and K.Total nutrient content >=: 45(%), available phosphorus content >=: 12(%), moisture content≤: 3(%), effective potassium content >=: 18(%), total nitrogen content >=: 15(%);
4. without soil fertility quality soil (hereinafter referred to as process 4).
(3) fertilizer applies mode
Inorganic composite fertilizer (process 3) process of topdressing, in transplanting after 2 weeks, tomato seedling restore normal growth, and starting to apply inorganic composite fertilizer sowing amount is 20g/ basin, dig two seedlings centres spread manuer in holes into.
Fertilizer-chicken manure (process 2) and bio-bacterial manure of the present invention (process 1) all apply as seed manure, namely when transplanting tomato seedling, respectively by fertilizer-chicken manure and bio-bacterial manure of the present invention with transplant mixing with soil, fertilizer-chicken manure and mixing with soil ratio are 1:3, biological bacteria of the present invention and mixing with soil ratio are 1:7, then load flowerpot, transplant tomato seedling.
(4) manage
After transplanting, under 4 kinds of process are placed in same environment, normal management, no longer enriches any fertilizer, observes tomato growing way weekly.
(5) investigation and result
Biomass measuring: when tomato grows to 6 leaf, measures tomato plant height and biomass.After different modes process, tomato growing way is shown in Fig. 4.
From table 5 experimental result, the soil capsicum growing way of bio-bacterial manure process of the present invention is vigorous, the speed of growth is obviously faster than other 3 kinds process, no matter be eggplant plant height or biomass are all the nearly more than 3 times of non-fertilizer treatment, be nearly 2 times without base composite fertilizer process, be also significantly better than fertilizer-chicken manure process simultaneously.Eggplant leaf thickness is all better than other 3 kinds process simultaneously, and color is also darker compared with other 3 kinds process.
The process of table 5 different fertilizer is on tomato plant height, biomass impact
| Process | 1 | 2 | 3 | 4 |
| Plant height (cm) | 23.4 | 18.9 | 12.5 | 8.3 |
| Biomass (weight in wet base, g) | 10.53 | 7.72 | 4.28 | 3.21 |
Different fertilizer process is on the impact of soil enzyme activities: when the 6th branch's leaf appears in tomato, get each process flowerpot soil respectively.Fetch earth earth time, each potted plant is carefully pulled out (weigh biomass with), fully mixed by flowerpot soil, every basin fetches earth 200g, puts into plastics bag and takes back laboratory, each soil enzyme activities of rapid test.
From the test-results of table 6, process 43 kinds of enzymes---namely urase, Phosphoric acid esterase and catalase activity are all apparently higher than other three kinds process, especially phosphatase activity and catalase activity are 2 times of the corresponding enzymic activity of no-fertilization, the soil of bacterial manure process is described, its transformation of soil nitrogen, phosphorus element transforms and organic matter transformation ability processes apparently higher than other.
The process of table 6 different fertilizer is on the impact (μ g/g) of tomato root soil enzymic activity
Bio-bacterial manure prepared by the present invention measures;
By testing bio-bacterial manure of the present invention according to NY884-2012 " biological organic fertilizer " national standard of The Ministry of Agriculture of the People's Republic of China, MOA's issuing and implementation on September 1st, 2012, can be found out by detected result, the indices of bio-bacterial manure of the present invention is surveyed apparently higher than the national standard just issued and implemented soon, and microbial inoculum particulate living bacteria count after 12 months storages still can reach 10
11cfu/g, test-results is in table 7, table 8:
Table 7 bio-bacterial manure quality measurements of the present invention
Table 8 bio-bacterial manure living bacteria count of the present invention detected result (unit cfu/g)
Claims (2)
1. a preparation method for high fertility long term effective resisting verticillium bio-bacterial manure is obtained than compound according to the quality of 90:1 bacterial manure carrier granule and microbial inoculum particulate;
Raw materials and the proportioning thereof of described bacterial manure carrier granule are:
50wt% chicken manure
20wt% colliery powder
15wt% crushing straw
15wt% wilkinite
The raw materials of above-mentioned bacterial manure carrier granule is ground into one by one 20 object fine powder batchings, to add in stirrer in above-mentioned component design ratio and sequencing and add binding agent and stir, add water fully mixing, control water content lower than 25wt%, extruder grain, dries 15min to kill pathogenic bacterium for 80 DEG C; Namely bacterial manure carrier granule is obtained after cooling;
Described microbial inoculum particulate is by first actication of culture, spreads cultivation, the step of rear mixing granulation and obtaining, specific as follows:
(1) activation of bacterial classification: by inclined-plane get respectively bacillus megaterium J1 (
bacillus megatherium), bacillusmusilaginosiengineering J2 (
bacillus mucilaginosus) and streptomyces rimosus J3(
streptomyces rimosus) respective access liquid activation medium in activate, thus obtain the culture material after three kinds of activation;
Activation culture based formulas, to add 1000ml H
2the substratum meter of O is specific as follows:
1. bacillus megaterium J1 activation medium: Semen Maydis powder 5.5g, bean cake powder 5.5g, NaCl 5.0g, H
2o 1000mL, pH 7.0 ~ 7.2, culture condition: 30 DEG C, cultivate under pH7.0;
2. bacillusmusilaginosiengineering J2 activation medium: bean cake powder 5.0g, poach soil extract 100ml, CaCO
30.1g, Na
2hPO
42.0g, MgSO
40.5g, FeCl
30.005g, H
2o 1000mL, pH7.0 ~ 7.2, culture condition: 30 DEG C, pH7.0 ~ 7.2 time cultivation;
3. streptomyces rimosus J3 activates PDA substratum: potato 200g, sucrose 20g, H2O 1000mL, pH7.0 ~ 7.2, culture condition: 28 DEG C, cultivate under natural pH;
(2) the spreading cultivation of bacterial classification: mixed according to the mass ratio of 1:2 with the raw materials of mixed bacterial manure carrier granule respectively by the culture material after above-mentioned three kinds of activation, at 18 ~ 25 DEG C, solid fermentation 3-5 days, obtains fermentation material; Be bacterial classification again with fermentation material, inoculate the raw materials of mixed bacterial manure carrier granule, mix according to the mass ratio of 1:10, at 18 ~ 25 DEG C, solid fermentation 15 days, finally obtains the microbial inoculum of three kinds of fermenting-ripenings;
(3) mixing granulation: the streptomyces rimosus J3 microbial inoculum of the bacillus megaterium J1 microbial inoculum of fermenting-ripening step (2) obtained, the bacillusmusilaginosiengineering J2 microbial inoculum of fermenting-ripening and fermenting-ripening is according to after volume ratio 1:2:2 mixing, add water after it is mixed with binding agent and be stirred to water content lower than 25wt%, extruder grain becomes the particulate of particle diameter≤1mm, cool after 75 DEG C of oven dry 15min, obtain microbial inoculum particulate.
2. high fertility long term effective resisting verticillium bio-bacterial manure, it is characterized in that according to the obtained high fertility long term effective resisting verticillium bio-bacterial manure of method described in claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310618874.9A CN103641605B (en) | 2013-11-26 | 2013-11-26 | High-fertility long-acting anti-verticillium-wilt biological bacterial fertilizer and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310618874.9A CN103641605B (en) | 2013-11-26 | 2013-11-26 | High-fertility long-acting anti-verticillium-wilt biological bacterial fertilizer and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103641605A CN103641605A (en) | 2014-03-19 |
| CN103641605B true CN103641605B (en) | 2015-02-11 |
Family
ID=50246940
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310618874.9A Active CN103641605B (en) | 2013-11-26 | 2013-11-26 | High-fertility long-acting anti-verticillium-wilt biological bacterial fertilizer and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103641605B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105016839A (en) * | 2014-04-18 | 2015-11-04 | 河北农业大学 | Nutritional biological tablet |
| CN105016838A (en) * | 2014-04-18 | 2015-11-04 | 河北农业大学 | Biocontrol bacterium nutritional tablet |
| CN105018383B (en) * | 2015-07-27 | 2018-10-02 | 江西省林业科学院 | A kind of complex microbial inoculum and method improving the Coal Wasting Lands surrival rate of afforestation |
| CN107245461B (en) * | 2017-05-31 | 2020-09-29 | 北方民族大学 | Bacillus amyloliquefaciens B6 and application thereof |
| CN107523524B (en) * | 2017-10-12 | 2018-08-14 | 天津坤禾生物科技集团股份有限公司 | A kind of composite bacteria agent and its preparation method and application of prevention tomato verticillium wilt |
| CN107721640A (en) * | 2017-11-20 | 2018-02-23 | 芜湖继伟农业有限公司 | A kind of preparation method of the charcoal base microbe slow release fertilizer of EM bacterium fermented stalk |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1740122A (en) * | 2004-08-27 | 2006-03-01 | 阮东 | Composite microbial fertilizer with coal gangue as matrix and its production process |
| CN101195549A (en) * | 2007-11-30 | 2008-06-11 | 上海绿乐生物科技有限公司 | Method for producing multifunctional biological fertilizer |
| CN102515954A (en) * | 2011-12-16 | 2012-06-27 | 上海绿乐生物科技有限公司 | Special biological organic fertilizer for verticillium wilt and production method for same |
-
2013
- 2013-11-26 CN CN201310618874.9A patent/CN103641605B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1740122A (en) * | 2004-08-27 | 2006-03-01 | 阮东 | Composite microbial fertilizer with coal gangue as matrix and its production process |
| CN101195549A (en) * | 2007-11-30 | 2008-06-11 | 上海绿乐生物科技有限公司 | Method for producing multifunctional biological fertilizer |
| CN102515954A (en) * | 2011-12-16 | 2012-06-27 | 上海绿乐生物科技有限公司 | Special biological organic fertilizer for verticillium wilt and production method for same |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103641605A (en) | 2014-03-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104560828B (en) | Microbial agent and application in prospect of passivating heavy metals of soil | |
| CN102432399B (en) | Biological organic fertilizer for improving stress resistance and output of paddy rice and preparation method thereof | |
| CN102876576B (en) | Acidic soil conditioner, and preparation method and application thereof | |
| CN102816005B (en) | Peanut special microbial fertilizer and production method thereof | |
| CN103641605B (en) | High-fertility long-acting anti-verticillium-wilt biological bacterial fertilizer and preparation method thereof | |
| CN109369269B (en) | Biochar compound microbial fertilizer for inhibiting soil-borne diseases and preparation method thereof | |
| CN103539535B (en) | Active biological matrix product specially used for culture of cucumber seedlings | |
| CN105462878B (en) | A kind of efficient decomposing agent of feces of livestock and poultry and its fermentation process | |
| CN103992184B (en) | A kind of biological organic fertilizer | |
| CN104560045B (en) | Microbial bacterial agent and chemical fertilizer excessively use growth-promoting secondary saline and alkaline and prone soil improved application | |
| CN103848698A (en) | Biological organic fertilizer prepared by aerobic fermentation of biogas residue and preparation method thereof | |
| CN105085121B (en) | A kind of composite microbiological fertilizer | |
| CN102491802A (en) | Tea seed cake biological organic fertilizer and production method thereof | |
| CN104276874A (en) | Microorganism-enzyme powdery composite biological preparation for promoting crop yield increase | |
| CN104818233B (en) | One plant of bacillus containing Death Valley and its functional form vegetables nursery bio-matrix of preparation | |
| CN102167631B (en) | Plug seedling matrix and application thereof | |
| CN103966148A (en) | Vegetable waste composting bacterial agent, using method thereof and organic substrate prepared from vegetable waste composting bacterial agent | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN106047764A (en) | Low-temperature beneficial microorganism preparation product applied to straw application | |
| CN110305671A (en) | One kind is for facilities vegetable continuous cropping soil biological modification agent and its preparation and application method | |
| CN107244961A (en) | The method that rice seedling culture medium is produced as raw material using Jasmine slag, tea dust | |
| CN106673866A (en) | Bacillus subtilis microbial fertilizer and preparation method thereof | |
| CN102827774B (en) | Microbial inoculum and application thereof in improvement of facility agriculture | |
| CN107473786A (en) | A kind of method that rice straw returning to the field improves soil | |
| CN110092685A (en) | A kind of disease-resistant expelling parasite biological organic fertilizer and its application in improvement alkaline land soil |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 116600 science and technology office, Dalian Nationalities University, No. 18 Liaohe West Road, Dalian economic and Technological Development Zone, Liaoning Patentee after: DALIAN MINZU University Address before: 116600 science and technology office, Dalian Nationalities University, No. 18 Liaohe West Road, Dalian economic and Technological Development Zone, Liaoning Patentee before: DALIAN NATIONALITIES University |
|
| CP01 | Change in the name or title of a patent holder | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231121 Address after: 010000 No. 2, building 1, gengyu fertilizer plant, Bayan Town, Saihan District, Hohhot City, Inner Mongolia Autonomous Region Patentee after: Inner Mongolia fengzhidao Ecological Engineering Co.,Ltd. Address before: 401120 No.107 dataguzhong Road, Xiantao street, Yubei District, Chongqing Patentee before: Chongqing Super Star Technology Co.,Ltd. Effective date of registration: 20231121 Address after: 401120 No.107 dataguzhong Road, Xiantao street, Yubei District, Chongqing Patentee after: Chongqing Super Star Technology Co.,Ltd. Address before: 116600 science and technology office, Dalian Nationalities University, No. 18 Liaohe West Road, Dalian economic and Technological Development Zone, Liaoning Patentee before: DALIAN MINZU University |
|
| TR01 | Transfer of patent right |