CN104276874A - Microorganism-enzyme powdery composite biological preparation for promoting crop yield increase - Google Patents
Microorganism-enzyme powdery composite biological preparation for promoting crop yield increase Download PDFInfo
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The invention discloses a microorganism-enzyme powdery composite biological preparation for promoting crop yield increase. The composite biological preparation is composed of, by weight, 30% of multiple microbial agents, 21% of multiple biological enzyme preparations, 25% of a microbial nutritional agent, 7% of seaweed, 3% of Fucus plant active ingredients, 3% of sodium humate, 2% of mordenite, 2% of dolomite and the balance moisture. The invention provides the microorganism-enzyme composite biological preparation for promoting high quality and high yield production of crops, and the composite biological preparation is formed by compounding of pure natural preferred strains and a variety of biological enzymes. Integrating the advantages of inoculants and enzyme preparations, the composite biological preparation has the functions of improving the soil structure, antagonizing soil pathogenic bacteria, fixing nitrogen, and dissolving phosphorus and potassium, releases iron, magnesium, manganese, zinc, sulfur, boron, calcium, silicon and a variety of medium trace elements in soil, balances absorption of nitrogen, phosphorus and potassium, removes fertilizer damage, secretes a variety of bioactive substances, enhances crop stress resistance, and can reach the effects of enhancing the yield and quality of crops.
Description
Technical field
The present invention relates to one and have fertilizing soil effect, and can promote the bacteria agent of increasing crop yield and the production technique of biological enzyme formulation, particularly one promotes increasing crop yield microorganism and enzyme powder composite biological agent.
Background technology
Grain is the basis of state Economic development and social stability, and grain is as a kind of important strategic materials, and it is directly connected to economic security and the national security of foreign country, and therefore grain security becomes the important component part of national security naturally.There are 18.26 hundred million mu of arable lands in China, but grain acreage is less than 1,600,000,000 mu, and the trend that people subtracts with increasing is difficult to containment.National agricultural is in the development of these decades, due to the pressure of increases in grain production, China have to improve agricultural land multiple crop index, increase the input amount of chemical fertilizer and agricultural chemicals to increase crop yield, this makes farmland becoming barren in varying degrees, degenerate, grain quality reduces, agriculture production cost increases, and China quietly enters the ranks of ternational grain net importer.The cultivated area of continuous reduction, low benefit of planting foods, the natural disaster taken place frequently, beaten again and again the alarm bell of grain security especially.In agriculture production, the usage quantity of chemical fertilizer and agricultural chemicals increases year by year, causes the problems such as the Soil degradation deterioration of the ecological environment, constitutes a threat to and challenge agricultural product security and agricultural sustainable development.
In order to improve the situation that China's grain faces at present, the exploitation of microbial technique research and microbial preparation brings wide prospect.At present biological organic fertilizer and bio-bacterial manure have had certain development and application on the market, but these bio-feritlizers to there is bacterial classification single, effect is simple, and the problem such as involve great expense, and limits penetration and promotion.Need the situation that more effect is comprehensive, bacterial classification is complete, current farmland improved by the biotechnological formulation of low cost and safety and environmental protection and farm crop face, and then advance the Sustainable development of domestic Organic farming.
Summary of the invention
For making up the weak point of some biological organic fertilizers and microbial-bacterial fertilizer in the market, the invention provides and a kind ofly gathered the advantage of traditional bacterial manure and zymin, microorganism and enzyme compound have been made a kind of promotion increasing crop yield microorganism and enzyme powder composite biological agent.
The present invention's one promotes increasing crop yield microorganism and enzyme powder composite biological agent, comprise multiple-microorganism microbial inoculum, multiple biological enzyme formulation, microbial nutrition agent, marine alga, black wrack vegetable active element, Sodium salts humic acids, mordenite, rhombspar and water, its part by weight is: 30% multiple-microorganism microbial inoculum; More than 21% kind of biological enzyme formulation; 25% microbial nutrition agent; 7% marine alga; 3% black wrack vegetable active element; 3% Sodium salts humic acids; 2% mordenite; 2% rhombspar; All the other are moisture.
Described multiple-microorganism microbial inoculum comprises dynamic coccus Planomicrobium chinense, pichia spp Pichia sp, Nitrosococcus Nitrosococcus, nitrated thorn bacterium Nitrospina, streptococcus faecium Streptococcus feacalis, soil bacteria Alternaria sp, blue-green algae Cyanobacteria, blown-ball Azotobacter Azotobacter chrooccum, cow dung branch spore Cladosporium atercorarium, purple bacteria purple bacteria, seta shell Chaetomium spinosum, India hair shell Chaetomium indicum, bacillus licheniformis Bacillus licheniformis, bacillus megaterium Bacillus megaterium, Actinomycesa lmadurae Actinomadura sp, streptomyces cellulosae Streptomycescellulose, it consists of weight ratio is in order 3: 2: 1: 2: 3: 2: 4: 3: 3: 2: 3: 4: 2: 3: 5: 2.
Described multiple biological enzyme formulation comprises cellulase, hemicellulase, polygalacturonase, lipase, steapsase, proteolytic enzyme, ALPH amylase, BETA amylase, BETA dextranase; It consists of weight ratio is in order 2: 1.5: 2: 1: 1.5: 2: 2: 1: 1.5.
Described microbial nutrition agent comprises bean powder cake, cavings, sweet potato flour, peat, wheat bran, peat, vitamins B
1, vitamins C; It consists of order weight ratio 20: 20: 15: 15: 15: 10: 3: 2.
The preparation process of described composite biological agent is as follows:
1, multiple-microorganism bacterial classification and multiple biological enzyme are selected:
Multiple biological enzyme of the present invention is that market is bought, and the former bacterial classification of various microorganisms in formula is all introduced from Chinese agriculture Microbiological Culture Collection administrative center (ACCC), ensure that survival and the quality of a bacterial classification.
2, the difference fermentative production of bacterial classification
Carry out respectively cultivating according to above-mentioned 16 kinds of strain bio habits, fermentative production.
3, multiple-microorganism microbial inoculum microbial inoculum is composite
Viable bacteria inspection is carried out to the various microorganism fermented, according to the obtained composite fungus agent of various microbial strains part by weight mixing.
4, multiple biological enzyme formulation is composite
Multiple biological enzyme formulation (meet and close national light industry standard), cellulase, hemicellulase, polygalacturonase, lipase, steapsase, proteolytic enzyme, ALPH amylase, BETA amylase, BETA dextranase are the obtained zymin of 2: 1.5: 2: 1: 1.5: 2: 2: 1: 1.5 mixing by weight.
5, the preparation of microbial nutrition agent
Bean powder cake, cavings, sweet potato flour, peat, wheat bran, peat, VITMAIN B1, vitamins C in order mass ratio 20: 20: 15: 15: 15: 10: 3: 2 mix, mix rear pulverizing, obtained microbial nutrition agent of sieving.
6, composite biological agent
Multiple-microorganism microbial inoculum and microbial nutrition agent are first mixed, then with multiple biological enzyme formulation, marine alga, black wrack vegetable active element, Sodium salts humic acids, mordenite, rhombspar and water by by weight ratio are: multiple-microorganism microbial inoculum: 30%, multiple biological enzyme formulation: 21%, microbial nutrition agent: 25%, marine alga: 7%, black wrack vegetable active element: 3%, Sodium salts humic acids: 3%, mordenite: 2%, rhombspar: 2%, all the other are moisture mixing, 100 mesh sieves were pulverized after cryodrying, it is 10 ~ 1,500,000,000/gram that sampling Detection (colony counting method) makes to contain living bacteria count in biotechnological formulation, obtain microorganism and enzyme powder composite biological agent.
The present invention is a kind of promotes that the increasing crop yield advantage of microorganism and enzyme powder composite biological agent is:
1, composite biological agent of the present invention, is composited by natural microbial and enzyme, not containing any pharmaceutical chemicals, also not containing transgenic product composition, can not cause secondary pollution.Adding of nutritive substance and mineral salt, make the nutrition of microorganism keep balance, be in high proliferation state all the time in the processing system, show unique superiority, reliability.Agriculture production can mix with fertilizer and use, irrigation water can be mixed use, can directly directly use when Soil tillage.Effect only relies on the related products such as simple microbial inoculum or simple biological enzyme to improve decades of times than conventional art.With its unique natural formula, possess the feature that effect is excellent, profitable, effectiveness is strong, purposes is wide, and active biologics is made solid powdery, be conducive to big area, production in enormous quantities, storing and application, effectively reduce use cost.
2, composite biological agent of the present invention, the cooperation of different microorganisms bacterial classification defines dominant microflora, increases profitable strain in soil, has the effect of antagonism pathogenic bacteria, produce different tethelin, stimulate the growth of crop, improve crop anti-adversity, there is fixed nitrogen, phosphorus decomposing and potassium decomposing function, promote absorption and the utilization of crop alimentary element, discharge the multiple medium trace element such as iron, magnesium, manganese, zinc, sulphur, boron, calcium, silicon in soil, the absorption of balance nitrogen, phosphorus, potassium, removes fertilizer damage simultaneously.Effective control farm crop physiological nutritional deficiency disease (early ageing, leaflet, Huang Ye, dehiscent fruit, evil mind, the rotten heart, watercore, bitter bean illness, contracting fruit etc.), improve chemical fertilizer utilization ratio, reduce fertilizer amount and fertilizer residue, there is efficient recovery soil fertility, reduce soil compaction, improve soil supplying nutrient capability, thus make farm crop reach the effect of volume increase raising quality.
3, composite biological agent of the present invention, the application of enzyme improves the decomposition of the difficult organism, stalk and the various organic waste that decompose in farmland, and for microorganism creates good propagation environment early stage, playing a role in microorganism provides good guarantee early stage.Promote that crop root part is to nutritive substance, can manufacture and assist Crop to utilize multiple nutrients material simultaneously, improve the drawback that the decomposition rate in early stage of biotechnological formulation is slow.
4, composite biological agent of the present invention, the use of microbial nutrition agent maintains microorganism in use quick active, nutritive substance and breeding environment must be obtained for microorganism provides, provide guarantee for microorganism index grows and occupy flora advantage in soil.Microorganism produces a large amount of secondary metabolites in growth and breeding process, and these products can promote the formation of soil aggregate.The formation of crumb structure makes that soil becomes loose, soft, preserve moisture and fertility performance strengthens, and water, gas, heat are coordinated more, are conducive to water conservation, fertilizer conservation, ventilation and promotion root growth and development, for farm crop provide comfortable growing environment.
5, composite biological agent of the present invention is by the Application of composite of microorganism and biological enzyme, not only inhibit the breeding of pathogenic bacteria in soil by secretory product in microbial activities process but also have stimulated the growth of crop root part, the environment of soil and farm crop is improved again at enzyme and microorganism acting in conjunction, improve the speed of growth and the resistance of farm crop, marine alga simultaneously, black wrack vegetable active element, Sodium salts humic acids, mordenite, desired nutritional material in plant growing process is provided with rhombspar, and promote that crop root part is grown and dietetic alimentation, make farm crop at nutritional sufficiency, the situation that environment is suitable for is issued to quality and improves, the effect that output increases.
Embodiment
Below in conjunction with embodiment, the present invention's one is promoted that increasing crop yield microorganism and enzyme powder composite biological agent are described in more detail.
One, multiple-microorganism bacterial classification and multiple biological enzyme are selected:
1) multiple-microorganism microbial inoculum comprises dynamic coccus Planomicrobium chinense, pichia spp Pichia sp, Nitrosococcus Nitrosococcus, nitrated thorn bacterium Nitrospina, streptococcus faecium Streptococcus feacalis, soil bacteria Alternaria sp, blue-green algae Cyanobacteria, blown-ball Azotobacter Azotobacter chrooccum, cow dung branch spore Cladosporium atercorarium, purple bacteria purple bacteria, seta shell Chaetomium spinosum, India hair shell Chaetomium indicum, bacillus licheniformis Bacillus licheniformis, bacillus megaterium Bacillus megaterium, Actinomycesa lmadurae Actinomadura sp, streptomyces cellulosae Streptomyces cellulose.
2) multiple biological enzyme formulation comprises cellulase, hemicellulase, polygalacturonase, lipase, steapsase, proteolytic enzyme, ALPH amylase, BETA amylase, BETA dextranase.
Multiple biological enzyme used in the present invention is market can be bought obtain, and the former bacterial classification of various microorganisms in formula is all introduced from Chinese agriculture Microbiological Culture Collection administrative center (ACCC), ensure that survival and the quality of a bacterial classification.
Two, the preparation of multiple-microorganism microbial inoculum:
Carry out respectively cultivating by biological nature according to above-mentioned 16 kinds of bacterial classifications, fermentative production.
1) inclined-plane seed culture
Multiple-microorganism microbial inoculum bacterial classification is single respectively carries out slant culture, cultivate 28 ~ 35 hours, then join (test tube) in sterilized water respectively, shake 30 minutes, treat to count with blood counting chamber evenly, make the bacterium number often propping up various bacterium in test tube be 1 × 10
8individual ml as deficiency, then can strengthen on the one hand and connect bacterium amount, can incubation time be lengthened on the other hand), inspection obtains bacteria suspension without after miscellaneous bacteria.
2) first order seed is cultivated
The bacteria suspension that the bacteria suspension of various bacterial classification respectively gets 1ml puts into 250ml triangular flask respectively, every triangular flask puts into nutrient solution, shaking table (180 revs/min) 30 DEG C is cultivated 30 ~ 38 hours, and with counting method of blood cell counting, in often kind of microbial inoculum, bacterium number content reaches 1 × 10
8individual/ml, inspection is without obtaining bacterial classification seed liquor after miscellaneous bacteria;
3) secondary seed is cultivated
Get bacterial classification seed liquor respectively and put into 500ml triangular flask, connecing bacterium amount is 10%, cultivates respectively, and shaking table (revolution 180 revs/min) 30 DEG C is cultivated 18 ~ 25 hours, and pH value is 6.0 ~ 7.5, and bacterium number reaches 7 ~ 9 × 10
9individual/ml, checks and obtains microbial inoculum without after miscellaneous bacteria.
4) fermentation culture
Getting microbial inoculum respectively inoculates into fermentor tank, and connecing bacterium amount is 10%, enlarged culturing in fermentor tank, and cultivate 18 ~ 24 hours for 30 DEG C, aeration-agitation is cultivated, and in fermented liquid, viable count reaches 5 ~ 8 × 10
10individual/ml, ensures without miscellaneous bacteria after inspection, obtains various multiple-microorganism bacterium liquid.
5) microbial inoculum is composite
The 16 kinds of microbiology class bacterium liquid obtained according to part by weight 3: 2: 1: 2: 3: 2: 4: 3: 3: 2: 3: 4: 2: 3: 5: 2 mixing in order of various microbial strains, viable bacteria obtains multiple-microorganism microbial inoculum after the assay was approved.
Three, the preparation of biotechnological formulation:
1) preparation of multiple biological enzyme formulation
Choose multiple biological enzyme formulation (meet and close national light industry standard), cellulase, hemicellulase, polygalacturonase, lipase, steapsase, proteolytic enzyme, ALPH amylase, BETA amylase, BETA dextranase, choose powdery enzyme preparation product, weight ratio is the obtained zymin of 2: 1.5: 2: 1: 1.5: 2: 2: 1: 1.5 mixing in order.
2) preparation of microbial nutrition agent
Bean powder cake, cavings, sweet potato flour, peat, wheat bran, peat, VITMAIN B1, vitamins C are pulverized respectively, crosses 100 mesh sieves, weight ratio 20: 20: 15: 15: 15: 10: 3: 2 mixes in order, mixes rear obtained microbial nutrition agent.
3) composite biological agent
Multiple-microorganism microbial inoculum and microbial nutrition agent are first mixed, then with multiple biological enzyme formulation, marine alga, black wrack vegetable active element, Sodium salts humic acids, mordenite, rhombspar and water mixing, 100 mesh sieves were pulverized after cryodrying, it is 20 ~ 3,000,000,000 Ge gram that sampling Detection (colony counting method) makes to contain living bacteria count in biotechnological formulation, obtains microorganism and enzyme powder composite biological agent.The weight ratio of constituents of powder composite biological agent is: multiple-microorganism microbial inoculum: 30%, multiple biological enzyme formulation: 21%, microbial nutrition agent: 25%, marine alga: 7%, black wrack vegetable active element: 3%, Sodium salts humic acids: 3%, mordenite: 2%, rhombspar: 2%, all the other are moisture.
Four, the application of composite biological agent in agriculture production:
1) application of composite bacteria agent on increasing crop yield:
After the several functions microorganism that complex micro organism fungicide includes enters soil, in growth and breeding process, produce a large amount of secondary metabolites, these products can promote the formation of soil aggregate.The formation of crumb structure makes that soil becomes loose, soft, preserve moisture and fertility performance strengthens, and water, gas, heat are coordinated more, are conducive to water conservation, fertilizer conservation, ventilation and promotion root growth and development, for farm crop provide comfortable growing environment, improve crop yield.2009 before the sowing of multiple kinds of crops farmland is carried out at agricultural technology extension center, Taiyuan City mu execute microbiobacterial agent 10 kilograms for field test and use standard, done Large Area of Crops effect of increasing production simultaneous test, experimental conditions; Since 2009, within continuous 3 years, successively to corn, 20 various crop such as Chinese sorghum, cereal, paddy rice, vegetables, herbage, carried out a large amount of experiments and demonstration, cumulative area reaches 2000 mu.Experimental result shows, the cash crop amounts of increase in production such as the increasing crop yields such as corn, Chinese sorghum, cereal, beans, paddy rice reach 15 ~ 20%, Lin Guo, castor-oil plant, beans reach 20%, and the nutrient content of product and mouthfeel significantly strengthen, detect through relevant department, meet national green product standard.
2) application of composite biological agent on agricultural land soil is fostered and apply fertilizer:
Powdery complex micro organism fungicide of the present invention has fixed nitrogen, phosphorus decomposing and potassium decomposing function, promote absorption and the utilization of crop alimentary element, discharge the multiple medium trace element such as iron, magnesium, manganese, zinc, sulphur, boron, calcium, silicon in soil, the absorption of balance nitrogen, phosphorus, potassium, removes fertilizer damage simultaneously.Before and after the agricultural land soil plantation crops of using microbial inoculum and vegetables results, the index content such as collection soil sample tests nitrogen, phosphorus, potassium wherein, organic, as following table:
| Farmland sample | N(％) | P(10 -6) | K(10 -6) | Organic carbon (%) | Organic (%) |
| Microbial preparation | 0.37 | 113.81 | 382.05 | 5.91 | 12.59 |
| Farm manure | 0.33 | 103.02 | 348.34 | 5.22 | 11.80 |
| Chemical fertilizer | 0.31 | 101.67 | 267.52 | 4.89 | 9.18 |
| Tradition bacterial manure | 0.30 | 103.68 | 282.10 | 5.16 | 10.92 |
| Element soil (not applying fertilizer) | 0.22 | 61.36 | 215.34 | 3.47 | 6.58 |
As can be seen from the above table, used the Nitrogen In Soils of complex micro organism fungicide, phosphorus, potassium, organic equal size has raising in various degree, and soil is all without the phenomenon that hardens, this illustrates that this efficient biologic-organic fertilizer can improve the physico-chemical property of soil, improve the fertility of soil, improve the structure of soil, be conducive to the growth of farm crop.Complex micro organism fungicide can significantly improve the content of soil with organic matter, can promote the release of Nutrient Elements in Soil, improves farm crop to the utilising efficiency of nutritive element, has good improvement of soil fertility effect.
3) application of composite biological agent on agriculture disease resistance:
This complex micro organism fungicide has carried out the planting experiment of multi items in multiple area, effective control farm crop physiological nutritional deficiency disease (early ageing, leaflet, Huang Ye, dehiscent fruit, evil mind, the rotten heart, watercore, bitter bean illness, contracting fruit etc.), improve chemical fertilizer utilization ratio, reduce fertilizer amount and fertilizer residue, there is efficient recovery soil fertility, reduce soil compaction, improve soil supplying nutrient capability, strengthen crop degeneration-resistant border ability.Significantly can promote the growth of farm crop, strengthen the ability of drought-resistant, resistant to lodging, the disease and insect resistance of farm crop, ensure in fertility in each of crop growth sufficient in period, improve crop quality, overcome soil compaction that Single fertilizer causes simultaneously, soil fertility declines, remain the unfavorable factors such as pest increases in farming object, thus reach increasing both production and income object.
Through showing of a large amount of lands for growing field crops kind value experiment: the application of this composite bacteria agent on corn, the highest amount of increase in production reaches 25.8%, make that corn is early emerged, jointing, heading stage, the ripening stage in advance, there is drought resisting, the excellent results such as resistant to lodging.
The planting experiment of winter wheat shows, can make that its seedling is neat, Miao Zhuan, increase tiller number, and improve drought-resistant ability, especially to prevention gaeumannomyces graminis disease successful, preventive effect reaches more than 95%, improves output 10-15%.The planting experiment of cucumber, potato class, celery shows all have effect of increasing production, also shows the effects such as resistant to lodging, disease-resistant, drought resisting simultaneously.
In sum, can find out, the aspects such as this complex micro organism fungicide has increasing both production and income, improvement soil, preventing disease and pest, resistant to lodging have excellent effect to farm crop.
Five, biotechnological formulation directly imposes on the experiment in farmland
The planting experiment that the farmland that this biotechnological formulation has carried out multi items in each department is directly used, significantly can promote the growth of farm crop, strengthen the ability of drought-resistant, resistant to lodging, the disease and insect resistance of farm crop, ensure in fertility in each of crop growth sufficient in period, improve crop quality, overcome soil compaction that Single fertilizer causes simultaneously, soil fertility declines, remain the unfavorable factors such as pest increases in farming object, thus reach increasing both production and income object.
Through showing of a large amount of land for growing field crops kind value experiments: the application of this biotechnological formulation on corn, the highest amount of increase in production reaches 21.7%, input-output ratio is 1: 10 ~ 35, make that corn is early emerged, jointing, heading stage, the ripening stage in advance, there is drought resisting, the excellent results such as resistant to lodging:
The planting experiment of winter wheat shows, can make that seedling is neat, Miao Zhuan, increase tiller number, and improve drought-resistant ability, especially to prevention gaeumannomyces graminis disease successful, preventive effect reaches more than 96%, improves output 11 ~ 13%.The planting experiment of cucumber, potato class, celery shows all have effect of increasing production, also shows the effects such as resistant to lodging, disease-resistant, drought resisting simultaneously.
But experiment also proves, above-mentioned composite biological agent only just has obvious effect on the soil that organic matter enriches, its reason only has under these conditions, the breeding that various probioticss in composite biological agent are just likely a large amount of, decomposing organic matter, activation nutritive element and produce the nutrient of a large amount of various adaptation crop growths, suggestion and farm manure mix to be used.
In sum, composite biological agent involved in the present invention adopts pure natural bacterial classification and enzyme to be composited, and directly or indirectly provides nutritive element agriculture production to farm crop thing, recovers soil micro-ecosystem balance, edatope and crops quality are improved.Can biological degradation organic, accelerate soil extensive use of chemical fertilizer effect; Purification soil, particularly suitable for cleaning uses the soil of perbromide, sterilant and weedicide; Strengthen the ability of farm crop, opposing disease and pest; Improve root system to the growth of the absorption enhancement farm crop of trace element; The natural fertility of soil can be recovered, be of value to the fast degradation of chemical pollutant, improve plant resistant boisterous ability and help it to reduce survival pressure because harsh climate causes and injury, advance the development and production of China's agricultural green product, make farm crop from quality and quantitatively improve production capacity.
The above; be only the present invention's preferably embodiment, but protection scope of the present invention is not limited thereto, is anyly familiar with those skilled in the art in the technical scope that the present invention discloses; the change that can expect easily or replacement, all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claim.
Claims (5)
1. promote increasing crop yield microorganism and an enzyme powder composite biological agent, it is characterized in that: described composite biological agent comprises multiple-microorganism microbial inoculum, multiple biological enzyme formulation, microbial nutrition agent, marine alga, black wrack vegetable active element, Sodium salts humic acids, mordenite, rhombspar He Shui, its formulation weight ratio are as follows:
Multiple-microorganism microbial inoculum: 30%;
Multiple biological enzyme formulation: 21%;
Microbial nutrition agent: 25%;
Marine alga: 7%;
Black wrack vegetable active element: 3%;
Sodium salts humic acids: 3%;
Mordenite: 2%;
Rhombspar: 2%;
Water: add to 100%.
2. one according to claim 1 promotes increasing crop yield microorganism and enzyme powder composite biological agent, it is characterized in that: described multiple-microorganism microbial inoculum component comprises by weight:
Dynamic 3 parts, coccus;
Pichia spp 2 parts;
Nitrosococcus 1 part;
Nitrated thorn bacterium 2 parts;
Streptococcus faecium 3 parts;
Soil bacteria 2 parts;
Blue-green algae 4 parts;
Blown-ball Azotobacter 3 parts;
Cow dung branch spore 3 parts;
Purple bacteria 2 parts;
3 parts, seta shell;
4 parts, India's hair shell;
Bacillus licheniformis 2 parts;
Bacillus megaterium 3 parts;
Actinomycesa lmadurae 5 parts;
Streptomyces cellulosae 2 parts.
3. one according to claim 2 promotes increasing crop yield microorganism and enzyme powder composite biological agent, it is characterized in that: described multiple biological enzyme formulation component comprises by weight:
Cellulase 2 parts;
Hemicellulase 1.5 parts;
Polygalacturonase 2 parts;
1 part, lipase;
Steapsase 1.5 parts;
2 parts, proteolytic enzyme;
ALPH amylase 2 part;
BETA amylase 1 part;
BETA dextranase 1.5 parts.
4. one according to claim 3 promotes increasing crop yield microorganism and enzyme powder composite biological agent, it is characterized in that: described microbial nutrition agent component comprises by weight:
Bean powder cake 20 parts;
Cavings 20 parts;
Sweet potato flour 15 parts;
Peat 15 parts;
15 parts, wheat bran;
10 parts, peat;
Vitamins B
13 parts;
Vitamins C 2 parts.
5. one according to claim 4 promotes increasing crop yield microorganism and enzyme powder composite biological agent, it is characterized in that: the preparation process of described composite biological agent is as follows:
1) described microbial strains and biological enzyme is selected by recipe requirements purchase;
2) carry out respectively cultivating according to described 16 kinds of strain bio habits, fermentative production bacterial classification;
3) viable bacteria inspection is carried out to the various microorganism fermented, according to the obtained multiple-microorganism microbial inoculum of described multiple-microorganism microbial inoculum component ratio mixing;
4) multiple biological enzyme formulation is prepared by described multiple biological enzyme formulation component ratio;
5) by described microbial nutrition agent component ratio preparing microorganism nutrition agent;
6) by described composite biological agent formula rate preparation composite biological agent, pulverized 100 mesh sieves after cryodrying, viable bacteria is up to the standards to obtain composite biological agent.
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