CN106916021A - A kind of double source humic acid biological fertilizer and preparation method and application - Google Patents
A kind of double source humic acid biological fertilizer and preparation method and application Download PDFInfo
- Publication number
- CN106916021A CN106916021A CN201710236114.XA CN201710236114A CN106916021A CN 106916021 A CN106916021 A CN 106916021A CN 201710236114 A CN201710236114 A CN 201710236114A CN 106916021 A CN106916021 A CN 106916021A
- Authority
- CN
- China
- Prior art keywords
- humic acid
- double source
- biological fertilizer
- acid biological
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of double source humic acid biological fertilizer and preparation method and application.The double source humic acid biological fertilizer is with ganoderma lucidum mushroom bran, burdock leaf, weathered coal or peat, adjusting material, complex enzyme, composite bacteria agent as primary raw material, it is obtained through enzymolysis and microbial fermentation, chemical activation including weathered coal or peat, burdock leaf enzymolysis, then it is well mixed with ganoderma lucidum mushroom bran again, fermentation maturity is carried out using composite bacteria agent.The present invention is not only comprehensively utilized to ganoderma lucidum mushroom bran and burdock leaf, and biological humic and mineral resources humic acid Synergistic in bio-feritlizer, with improved soil, the effect for promoting plant growth, strengthening disease-resistant and anti-adversity ability, improve product quality.
Description
Technical field
The present invention relates to a kind of bio-feritlizer, more particularly to a kind of bio-feritlizer of double source humic acid and preparation method thereof with
Using belonging to agro-ecology fertilizer technology field.
Background technology
The application of chemical fertilizer promotes the fast development of China's agricultural, but long-term excessive use chemical fertilizer, result in soil plate
Knot, salination, acidifying, content of organic matter reduction, the problems such as pest and disease damage is serious.The rational application of fertilizer, reduces chemical fertilizer, enriches bio-feritlizer
It is the effective means for solving the above problems.Bio-feritlizer contain beneficial microbe, microbial metabolic products, organic matter etc. effectively into
Point, with promotion growth and development of plants, improve quality of agricultural product, enhancing plant stress-resistance ability, mitigation pest and disease damage, improved soil etc.
Effect.The application effect of microbial manure depends not only on the type and quantity of contained microorganism, additionally depends on contained organic matter
The source of matter, species and production method.Bio-feritlizer is produced using the Organic Ingredients rich in biostimulant and biological protective plant protecting agent,
Its application effect can be improved.
Humic acid is one of material base of soil fertility, including mineral resources humic acid and the major class of biological humic two.Mineral resources
Humic acid is mainly derived from the mineral resources such as lignite, weathered coal, peat, and because the sedimentary age is remote, reduction-state is high, active group
Few, activity is low, it is necessary to could be used through overactivation.Biological humic is the rotten plant that biomass material is produced through microbial fermentation
Acid, molecular weight is small, and functional group is more, and biological activity ratio's mineral resources humic acid is stronger.At present, what report was more is individually with biological rotten
The fertilizer product of phytic acid fertilizer or mineral resources humic acid, such as CN105110969A provides a kind of fertilizer based on biological humic and uses
Water conservation Synergistic slow release agent, by biological humic 40-70 parts, earthworm fibrinlytic enzyme 10-20 parts, micro- 15-25 part of complexing agent, gather
Acrylic acid 2-10 parts, dicyandiamide 1.0-2.0 parts, Nafusaku 0.4-1.2 parts.For another example CN105367204A provides a kind of water conservation and delays
Release fertilizer, the ratio of weight and number of each component is：Phosphorus, potassium fertilizer raw material 50-60, the cementing bonding material 20- of weathered coal humic acids
30, coated fertilizer 5-10, water-loss reducer 5-10, described nitrogen, phosphorus, potassium fertilizer raw material, nitrogen are urea, and phosphorus is MAP or phosphoric acid
Magnesium, potassium is potassium chloride, and described coated fertilizer is water microsolubility inorganic nutrients coated fertilizer, and the water-loss reducer is starch-series water conservation
Agent or polymeric retention agent.It follows that existing bio-fertilizer utilizes biological humic fertilizer or mineral resources humic acid, though can play certain
The effect of improved soil, but fail well by two kinds of humic acids of mineral resources humic acid and biological humic carry out organically combine so as to
Collaboration promotes to improve soil.
In recent years, the industry development of China's bio-feritlizer is rapid, but generally existing product homogeneity is serious, application effect is paid no attention to
The problems such as thinking.Product generally emphasizes the function of microorganism, and to the effect containing biostimulant and the Organic Ingredients of protective plant protecting agent
Pay attention to not enough.
The content of the invention
Part in view of the shortcomings of the prior art of the invention, there is provided a kind of double source humic acid biological fertilizer and its preparation
Method.
Term is explained：
u/g：Refer to the enzyme-activity unit number in every gram of complex enzyme formulation, i.e. enzyme activity.
cfu/g：Refer to the viable count in every gram of composite bacteria agent, cfu is CFU.
PDA slant mediums：PDA slant mediums, are the abbreviations to potato dextrose agar, formula：Horse
200 parts of bell potato, 20 parts of glucose, 15~20 parts of agar, 1000 parts of running water.
Nutrient agar slant medium：5.0 parts of peptone, 3.0 parts of powdered beef, 15.0 parts of agar, 1000 parts of running water.
Ganoderma lucidum mushroom bran：It is that ganoderma lucidum harvests the cultivation matrix discarded after fructification.
Technical scheme is as follows：
A kind of double source humic acid biological fertilizer, is made up of the raw material of following parts by weight of component：
40-60 parts of ganoderma lucidum mushroom bran, burdock leaf 20-30 parts, weathered coal or peat 20-30 parts, complex enzyme formulation 0.1-0.2
Part, composite bacteria agent 0.1-0.2 parts, adjusting material 0.1-0.2 parts；Wherein,
The complex enzyme formulation is by cellulase, pectase, zytase list enzyme preparation and filler glucose compounding
Form；
The composite bacteria agent is the compound bacteria containing candida tropicalis, Paenibacillus polymyxa and bacillusmusilaginosiengineering
Agent；
The adjusting material is dodecyl sodium sulfate and neopelex 1~2：1 weight than combination.
According to currently preferred, in the complex enzyme formulation, the enzyme activity of various enzymes is：Cellulase 10000-
20000u/g, pectase 5000-10000u/g, zytase 20000-30000u/g；More preferably：Cellulase
20000u/g, pectase 10000u/g, zytase 30000u/g.
According to currently preferred, candida tropicalis, Paenibacillus polymyxa and gel-shaped bud in the composite bacteria agent
The viable count of spore bacillus is：Candida tropicalis (1-2) × 109Cfu/g, Paenibacillus polymyxa (1-2) × 109Cfu/g, glue
Freeze sample bacillus (1-2) × 109cfu/g。
According to the present invention it is further preferred that the adjusting material be dodecyl sodium sulfate and neopelex=
1.5:1 weight ratio.The present invention can be adjusted effectively using the adjusting material that dodecyl sodium sulfate is combined with neopelex
The physico-chemical properties such as interfacial tension, the wellability of material are saved, is conducive to water toward infiltration in material, be conducive to microorganism to be connect with material
Touch, improve ferment effect.
Heretofore described candida tropicalis, Paenibacillus polymyxa and bacillusmusilaginosiengineering are existing bacterium
Kind.Further preferably, Chinese agriculture Microbiological Culture Collection administrative center, deposit number be ACCC20006 candida tropicalises,
ACCC03151 Paenibacillus polymyxas and ACCC10012 bacillusmusilaginosiengineerings.
In the present invention, the ganoderma lucidum mushroom bran is that ganoderma lucidum harvests the cultivation matrix discarded after fructification.The cultivation matrix master
If cotton seed hulls, velveteen, corncob, sawdust, crop straw, wheat bran etc..
In the present invention, described burdock leaf is the burdock leaf for air-drying or drying.
In the present invention, it is preferred that the content of organic matter of the weathered coal or peat is 75-85%, humic acid content is 45-
50%；Content in the present invention is mass percent, except specified otherwise.
Currently preferred, the composite bacteria agent is by candida tropicalis, Paenibacillus polymyxa, gel-shaped gemma bar
The strain of bacterium carries out actication of culture, Shaking culture and obtains seed liquor respectively, and fermented and cultured obtains zymotic fluid, is then spray-dried
To single bacterium preparation, then mixture is obtained in proportion with glucose filler.
According to the present invention, the compound method of the composite bacteria agent presses techniques well known.The present invention is provided
Preferred scheme.The method of the preparation of composite bacteria agent, including step：
(1) candida tropicalis single bacterium preparation is prepared
A, with PDA slant mediums by Candida tropicalis kind activate；
It is prepared by b, shake-flask seed:By mass parts, 15 parts of glucose is taken, 70 parts of corn flour, 10 parts of peptone, 5 parts of dusty yeast,
1000 parts of water, sterilizes 20-30 minutes under pH value 6.0-7.0,0.11MPa pressure, is made fluid nutrient medium A；After step a is activated
Candida tropicalis access the fluid nutrient medium A that is made, cultivate 24 in 28~32 DEG C, 150-180 revs/min of shaking flask
~36 hours；Obtain Candida tropicalis seed liquor；The viable bacteria content of seed liquor is 1 × 108Cfu/mL~5 × 108cfu/mL；
It is standby；
The preparation of c, zymotic fluid：Fluid nutrient medium A described in preparation process b, loads 1m3Fermentation tank in, coefficient is
Sterilized 20-30 minutes under 0.8,0.11MPa pressure, access the candida tropicalis seed liquor of step b cultures, inoculum concentration is 2-
5%, cultivated 20~24 hours under conditions of 28~32 DEG C, 150 revs/min of speed of agitator, throughput 0.5-1.0vvm；Obtain hot
Band Candida fermented liquid；The viable count of zymotic fluid is 1 × 109Cfu/mL~5 × 109cfu/mL；It is standby；
D, preparation single bacterium preparation：Preparation process c gained Candida tropicalis zymotic fluids are in 180 DEG C of inlet temperature, outlet
Under conditions of temperature 60 C, it is spray-dried, is obtained Candida tropicalis preparation；The viable bacteria of Candida tropicalis preparation
Number is 5 × 109Cfu/g~2.0 × 1010cfu/g。
(2) Paenibacillus polymyxa single bacterium preparation is prepared
A, with nutrient agar slant medium by Paenibacillus polymyxa actication of culture；
It is prepared by b, shake-flask seed:By mass parts, 20 parts of corn flour, 70 parts of bean cake powder are taken, 10 parts of dusty yeast, 1000 parts of water,
Sterilized 20-30 minutes under pH value 6.5-7.5,0.11MPa pressure, be made fluid nutrient medium B；How viscous class after step a is activated
Bacillus is accessed in the fluid nutrient medium B being made, and 20~24 is cultivated in 35~37 DEG C, 150-180 revs/min of shaking flask small
When；Obtain Paenibacillus polymyxa seed liquor；The viable bacteria content of seed liquor is 1 × 108Cfu/mL~5 × 108cfu/mL；It is standby；
The preparation of c, zymotic fluid：Culture medium B described in preparation process b, loads 1m3Fermentation tank in, coefficient is 0.8,
Sterilized 20-30 minutes under 0.11MPa pressure, access the Paenibacillus polymyxa seed liquor of step b cultures, inoculum concentration is 2-5%,
Cultivated 20~24 hours under conditions of 35~37 DEG C, 150 revs/min of speed of agitator, throughput 0.5-1.0vvm；Much glue class
Fermentation of bacillus liquid；The viable count of zymotic fluid is 1 × 109Cfu/mL~5 × 109cfu/mL；It is standby；
D, preparation single bacterium preparation：Preparation process c gained Paenibacillus polymyxa zymotic fluids are in 220 DEG C of inlet temperature, outlet
Under conditions of temperature 70 C, it is spray-dried, is obtained Paenibacillus polymyxa preparation；The viable bacteria of Paenibacillus polymyxa preparation
Number is 5 × 109Cfu/g~2.0 × 1010cfu/g。
(3) bacillusmusilaginosiengineering single bacterium preparation is prepared
A, with nutrient agar slant medium by bacillusmusilaginosiengineering actication of culture；
It is prepared by b, shake-flask seed:By mass parts, 30 parts of corn flour, 60 parts of bean cake powder are taken, 10 parts of dusty yeast, 1000 parts of water,
Sterilized 20-30 minutes under pH value 6.5-7.5,0.11MPa pressure, be made fluid nutrient medium C；Gel-shaped after step a is activated
Bacillus is accessed in the fluid nutrient medium C being made, and 20~24 is cultivated in 35~37 DEG C, 150-180 revs/min of shaking flask small
When；Obtain bacillusmusilaginosiengineering seed liquor；The viable bacteria content of seed liquor is 1 × 108Cfu/mL~5 × 108cfu/mL；It is standby；
The preparation of c, zymotic fluid：Culture medium C described in preparation process b, loads 1m3Fermentation tank in, coefficient is 0.8,
Sterilized 20-30 minutes under 0.11MPa pressure, access the bacillusmusilaginosiengineering seed liquor of step b cultures, inoculum concentration is 2-5%,
Cultivated 20~24 hours under conditions of 35~37 DEG C, 150 revs/min of speed of agitator, throughput 0.5-1.0vvm；Obtain gel-shaped
Fermentation of bacillus liquid；The viable count of zymotic fluid is 1 × 109Cfu/mL~5 × 109cfu/mL；It is standby；
D, preparation single bacterium preparation：Preparation process c gained bacillusmusilaginosiengineering zymotic fluids are in 220 DEG C of inlet temperature, outlet
Under conditions of temperature 70 C, it is spray-dried, is obtained bacillusmusilaginosiengineering preparation；The viable bacteria of bacillusmusilaginosiengineering preparation
Number is 5 × 109Cfu/g~2.0 × 1010cfu/g。
(4) preparation of composite bacteria agent
According to candida tropicalis preparation, Paenibacillus polymyxa preparation, bacillusmusilaginosiengineering preparation viable count, take
10-30 parts of Candida tropicalis agent, Paenibacillus polymyxa 10-30 parts, bacillusmusilaginosiengineering 10-30 parts of mixing, addition
Glucose gathers together enough 100 parts, is well mixed so that candida tropicalis (1-2) × 109Cfu/g, Paenibacillus polymyxa (1-2)
×109Cfu/g, bacillusmusilaginosiengineering (1-2) × 109Cfu/g, as described composite bacteria agent.
According to the present invention, a kind of preparation method of the double source humic acid biological fertilizer, including step：
(1) by proportioning, taking during weathered coal or peat add inorganic alkali solution carries out chemical activation；The weathered coal that must activate or
Peat；
(2) by proportioning, burdock leaf and complex enzyme are taken, it is first that complex enzyme is soluble in water, then be well mixed with burdock leaf, burdock
Leaf compares 1 with the weight of water：1~1.5,40-50 DEG C of accumulation 3-5h, is digested, and obtains burdock leaf zymolyte；
(3) by proportioning, take adjusting material and during composite bacteria agent dissolves in suitable quantity of water, mix, be subsequently adding living obtained in step (1)
The weathered coal or peat of change, burdock leaf zymolyte and ganoderma lucidum mushroom bran obtained in step (2), are well mixed, and obtain fermentation substrate；
(4) the above-mentioned fermentation substrate for making is put into solid-state fermenter, coefficient 0.35-0.45, between 35-45 DEG C
Have a rest ventilating aerobic fermentation 5-8d, then stands afterripening fermentation 10-20d；
(5) after fermentation ends, fermentation materials are obtained final product through air-drying, crushing.
With the method for the invention it is preferred to, in step (1), the weight of the weathered coal or peat and inorganic alkali solution
Than being 1：1~2；Chemical activation condition is：40-50 DEG C of accumulation 3-5h.It is further preferred that described inorganic alkali solution is quality
The KOH of fraction 5~15%.
With the method for the invention it is preferred to, in step (2), the water content of the fermentation substrate is 55-60% weight hundred
Divide ratio.
The application of double source humic acid biological fertilizer of the present invention, is widely used in fruit tree, vegetables and field crop, described big
Field crop is mainly including wheat, corn, paddy rice etc.；Double source humic acid biological fertilizer of the present invention is in apple, peach, grape, white
Be can be used on the crops such as dish, cucumber, cucurbita pepo, wheat, corn, paddy rice；Both can be used as base fertilizer, can also topdress uses.
Double source humic acid biological fertilizer consumption of the present invention is as follows：
When making base fertilizer：300~500 kilograms of every mu of vegetable crop, 5~15 kilograms of every plant of fruit tree, wheat, corn or paddy rice are every
200~400 kilograms of mu；
When topdressing：150~250 kilograms of every mu of vegetable crop, 5~15 kilograms of every plant of fruit tree, wheat, corn or paddy rice are every
100-200 kilograms of mu.
Technical characterstic of the invention and beneficial effect：
1st, double source humic acid biological fertilizer of the invention, creatively by the generation of biological humic and mineral resources humic acid
Bioactivation is fused in same production process so that contain mineral resources humic acid and biological humic in product simultaneously, wherein,
Mineral resources humic acid comes from weathered coal or peat；The biomass materials such as ganoderma lucidum mushroom bran, burdock leaf can turn in digest process through biology
Change forms biological humic.The humic acid structure of both separate sources is different, functionally in the presence of complementarity, is used in conjunction with, and has
There is improved soil, increase soil fertility, the effect such as enhancing plant disease-resistant, anti-adversity ability.
2nd, double source humic acid biological fertilizer of the invention, with ganoderma lucidum mushroom bran, burdock leaf biomass material through enzymolysis, fermentation shape
Into biological humic, weathered coal or peat provide precursor substance for the formation of biological humic, and the formation to biological humic has
Facilitation.On the other hand, biomass material can produce substantial amounts of microorganism, enzyme, energy, NADH etc. in digest process,
There is bioactivation to weathered coal, further increase the activation efficiency of mineral resources humic acid.Additionally, product of the present invention also contains
Beneficial microbe and its metabolite.
3rd, the main component of burdock leaf is the macromolecular polysaccharide material such as cellulose, xylan and pectic substance, present invention discover that
These materials can be carried out very according to complex enzyme formulation prepared by specific proportioning using cellulase, pectase and zytase etc.
Hydrolyze well, be conducive to the release of the intracellular organic matters such as burdock leaf Content of Chlorogenic Acid, synanthrin；While cellulose, xylan and pectic substance
After Deng macromolecular polysaccharide Substance P hydrolysis, oligosaccharide can be partially converted into；This is all conducive to improving the application of bio-feritlizer of the present invention
Effect.Crop is promoted preferably to grow.
4th, double source humic acid biological fertilizer prepared by the present invention is rich in chlorogenic acid protective plant protecting agent, to cucumber fusarium axysporum, capsicum epidemic disease
The plurality of plant diseases such as disease have certain prevention effect；Also contain burdock oligosaccharide simultaneously, can not only promote vegetable seeds to sprout
Hair and the growth of seedling, also can induce plant and improve its resistance to multiple diseases.On the other hand, burdock is planted as medicine-food two-purpose
Thing, processing industry mainly utilizes its root and son, burdock leaf to be taken as offal treatment, and the present invention is raw material from burdock leaf, also for
The discarded object of burdock processing industry provides the approach that a resourcebility is utilized.
5th, the present invention not only increases the level of comprehensive utilization of ganoderma lucidum mushroom bran and burdock leaf, is conducive to promoting edible mushroom and ox
The sound development of burdock industry, and the double source humic acid biological fertilizer invented, with improved soil, promote plant growth, increasing
Strong disease-resistant and anti-adversity ability, the effect of improve product quality, with significant economic benefit and social benefit.
6th, double source humic acid biological fertilizer of the invention can be widely used for fruit tree, vegetables and field crop, can both make base fertilizer
Use, can also topdress uses.
Specific embodiment
The present invention is further illustrated with reference to embodiment, can make those skilled in the art that this hair is more completely understood
It is bright, but do not limit the invention in any way.% described in embodiment is mass percent, except specified otherwise.
The ganoderma lucidum mushroom bran is that the mushroom bran material bag after ganoderma lucidum fruitbody harvesting removes outsourcing film, rejects putrid and deteriorated bacterium
Chaff, machinery is smashed and can be used；
Described burdock leaf is the burdock leaf for air-drying or drying.Reject mildew and rot, rotten leaf.
The cellulase enzyme preparation, pectase enzyme preparation, xylanase enzyme preparation are commercial products.Complex enzyme formulation
With prepare it is as follows：Extracting cellulose enzyme enzyme preparation, pectase enzyme preparation, xylanase enzyme preparation and filler glucose are made into
100 weight portions so that enzyme activity is in complex enzyme formulation：Cellulase 10000-20000u/g, pectase 5000-10000u/g,
Zytase 20000-30000u/g.
Described candida tropicalis, Paenibacillus polymyxa and bacillusmusilaginosiengineering, its original seed are bought in China
Agriculture Microbiological Culture Collection administrative center, its deposit number is respectively ACCC20006, ACCC03151 and ACCC10012.It is multiple
Close preparing for microbial inoculum as follows：By aforementioned preferred method of the present invention, by candida tropicalis, Paenibacillus polymyxa, gel-shaped bud
The strain of spore bacillus carries out actication of culture, Shaking culture and obtains seed liquor respectively, and fermented and cultured obtains zymotic fluid, then sprays dry
It is dry to obtain single bacterium preparation, 10~30 parts of Candida tropicalis agent, 10~30 parts of Paenibacillus polymyxa, gel-shaped gemma will be taken
10~30 parts of mixing of bacillus, addition glucose is gathered together enough 100 parts, is well mixed, obtains final product；So that the viable count in every gram of composite bacteria agent
For：Candida tropicalis (1~2) × 109Cfu/g, Paenibacillus polymyxa (1~2) × 109Cfu/g, bacillusmusilaginosiengineering
(1~2) × 109cfu/g。
Embodiment 1：A kind of double source humic acid biological fertilizer, is made up of the raw material of following parts by weight of component：
50 parts of ganoderma lucidum mushroom bran, 25 parts of burdock leaf, weathering coal dust 25,0.1 part of complex enzyme, 0.1 part of composite bacteria agent, adjusting material
0.1 part；
The complex enzyme formulation is by cellulase, pectase, zytase list enzyme preparation and filler glucose compounding
Form；The enzyme activity of various enzymes is in complex enzyme formulation：Cellulase 20000u/g, pectase 10000u/g, zytase
30000u/g。
The weathering coal dust, its content of organic matter is 75%, and humic acid content is 45%；
The composition of the adjusting material is：By weight percentage, dodecyl sodium sulfate 60%, neopelex
40%；
The viable count of the various bacterium in the composite bacteria agent is：Candida tropicalis 2 × 109Cfu/g, more viscous class gemma bar
Bacterium 2 × 109Cfu/g, bacillusmusilaginosiengineering 2 × 109cfu/g。
A kind of preparation method of the double source humic acid biological fertilizer, step is as follows：
(1) the weathered coal powder raw material of prescription amount is taken, by weight 1：1 KOH solution for adding mass fraction 10%, mixing is equal
Even, 45 DEG C of accumulation 4h complete chemical activation treatment；
(2) burdock leaf of prescription amount and the complex enzyme of prescription amount are taken, by burdock leaf：Water=1：1 weight ratio adds water, first will be multiple
Synthase is soluble in water, then is well mixed with burdock leaf, 45 DEG C of accumulation 4h, is digested.
(3) in the adjusting material and composite bacteria agent of prescription amount being dissolved in into suitable quantity of water, mix, then with the activation wind of above-mentioned acquisition
The ganoderma lucidum mushroom bran of powdered coal, burdock leaf zymolyte and prescription amount is well mixed, as fermentation substrate, controls its water content 55-
60%；
(4) the above-mentioned fermentation substrate for modulating is put into solid-state fermenter, coefficient 0.35-0.45, in 35-45 DEG C
Intermittent aeration aerobic fermentation 7d, then stands afterripening fermentation 10-20d；
(5) after fermentation ends, fermentation materials after air-drying, crushing, as double source humic acid biological fertilizer.
Embodiment 2：
A kind of double source humic acid biological fertilizer, is made up of the raw material of following parts by weight of component：
60 parts of ganoderma lucidum mushroom bran, 20 parts of burdock leaf, 20 parts of weathering coal dust, 0.2 part of adjusting material, 0.2 part of complex enzyme, composite bacteria agent
0.2 part；
The complex enzyme formulation is by cellulase, pectase, zytase list enzyme preparation and filler glucose compounding
Form；The enzyme activity of various enzymes is in complex enzyme formulation：Cellulase 10000u/g, pectase 5000u/g, zytase
20000u/g；
The weathering coal dust, its content of organic matter is 85%, and humic acid content is 50%；
The composition of the adjusting material is：By weight percentage, dodecyl sodium sulfate 60%, neopelex
40%；
The viable count of the various bacterium in the composite bacteria agent is：Candida tropicalis 1 × 109Cfu/g, more viscous class gemma bar
Bacterium 1 × 109Cfu/g, bacillusmusilaginosiengineering 1 × 109cfu/g；
The preparation method of the double source humic acid biological fertilizer, comprises the following steps that：
(1) the weathered coal powder raw material of prescription amount is taken, by weight 1：1 KOH solution for adding mass fraction 10%, mixing is equal
Even, 50 DEG C of accumulation 3h complete chemical activation treatment；
(2) burdock leaf of prescription amount and the complex enzyme of prescription amount are taken, by burdock leaf：Water=1：1.2 mass ratioes add water, and first will
Complex enzyme is soluble in water, then is well mixed with burdock leaf, 50 DEG C of accumulation 3h, is digested.
(3) in the adjusting material and composite bacteria agent of prescription amount being dissolved in into suitable quantity of water, mix, then with the weathered coal of above-mentioned acquisition
The ganoderma lucidum mushroom bran of powder, burdock leaf and prescription amount is well mixed, as fermentation substrate, controls its water content 55-60% weight percents
Than；
(4) the above-mentioned fermentation substrate for modulating is put into solid-state fermenter, coefficient 0.35-0.45, in 35-45 DEG C
Intermittent aeration aerobic fermentation 7d, then stands afterripening fermentation 10-20d；
(5) after fermentation ends, fermentation materials obtain final product double source humic acid biological fertilizer after air-drying, crushing.
Application test example
To verify the application effect of double source humic acid biological fertilizer of the present invention, the double source prepared using embodiment 1,2
Humic acid biological fertilizer has carried out field experiment in 10 years on raw Fuji apple trees.Apple tree 18 is chosen, 3 are randomly divided into
Group, every group 6, first group of double source humic acid biological fertilizer experiment process group prepared as embodiment 1, second group used as implementation
Double source humic acid biological fertilizer experiment process group prepared by example 2, the 3rd group is applied common organic fertilizer as a control group, described common
Organic fertilizer is prepared after being become thoroughly decomposed as raw material with manioc waste and cow dung, and the content of organic matter is 55%, and N P and K total nutrient is 6%.
Experiment process group applies kilogram/plant of double source humic acid biological fertilizer 8, is applied in the way of topdressing, before the florescence, in
The outer rim of tree crown upright projection, opens at the 8-10 of cave around fruit tree surrounding, deep 20-30 centimetres, double source humic acid fertilizer is uniformly applied
Enter.Control group applies kilogram/plant of common organic fertilizer 8 in the same way.Determine single fruit weight, the apple production index such as single plant yield with
The index of quality such as total reducing sugar, total acid, vitamin C, solid content, with the application effect of overall merit double source humic acid biological fertilizer
Really.Experimental result is as shown in table 1.
Influence of the fertilizer of humic acid biological containing double source of table 1 to apple yield and quality
Compared with traditional common organic fertilizer, using the apple tree single fruit weight and individual plant of double source humic acid biological fertilizer of the present invention
Yield increases, and fruit quality is obviously improved, and shows as total acid decline, and total reducing sugar, soluble solid and Vitamin C content are improved,
Sugar-acid ratio is improved.
Claims (10)
1. a kind of double source humic acid biological fertilizer, it is characterised in that be made up of the raw material of following parts by weight of component：
40-60 parts of ganoderma lucidum mushroom bran, burdock leaf 20-30 parts, weathered coal or peat 20-30 parts are complex enzyme formulation 0.1-0.2 parts, multiple
Close 0.1-0.2 parts, adjusting material 0.1-0.2 parts of microbial inoculum；Wherein,
The complex enzyme formulation is to form cellulase, pectase, zytase list enzyme preparation and filler glucose compounding；
The composite bacteria agent is the composite bacteria agent containing candida tropicalis, Paenibacillus polymyxa and bacillusmusilaginosiengineering；
The adjusting material is dodecyl sodium sulfate and neopelex 1~2：1 weight than combination.
2. double source humic acid biological fertilizer according to claim 1, it is characterised in that in the complex enzyme formulation, each enzyme
Enzyme activity be：Cellulase 10000-20000u/g, pectase 5000-10000u/g, zytase 20000-30000u/g；
Preferably, cellulase 20000u/g, pectase 10000u/g, zytase 30000u/g.
3. double source humic acid biological fertilizer according to claim 1, it is characterised in that vacation silk in the torrid zone in the composite bacteria agent
The viable count of yeast, Paenibacillus polymyxa and bacillusmusilaginosiengineering is：Candida tropicalis (1-2) × 109It is cfu/g, many
Viscous series bacillus (1-2) × 109Cfu/g, bacillusmusilaginosiengineering (1-2) × 109cfu/g。
4. double source humic acid biological fertilizer according to claim 1, it is characterised in that the adjusting material is dodecyl sulphur
Sour sodium and neopelex=1.5:1 weight ratio.
5. double source humic acid biological fertilizer according to claim 1, it is characterised in that described candida tropicalis, many
Viscous series bacillus and bacillusmusilaginosiengineering are all from Chinese agriculture Microbiological Culture Collection administrative center, deposit number difference
It is ACCC20006, ACCC03151 and ACCC10012.
6. the preparation method of double source humic acid biological fertilizer described in any one of claim 1-5, including step：
(1) by proportioning, taking during weathered coal or peat add inorganic alkali solution carries out chemical activation；The weathered coal or mud that must be activated
Charcoal；
(2) by proportioning, burdock leaf and complex enzyme are taken, it is first that complex enzyme is soluble in water, then be well mixed with burdock leaf, burdock leaf with
The weight of water compares 1：1~1.5,40-50 DEG C of accumulation 3-5h, is digested, and obtains burdock leaf zymolyte；
(3) by proportioning, take adjusting material and during composite bacteria agent dissolves in suitable quantity of water, mix, be subsequently adding the obtained activation of step (1)
Weathered coal or peat, burdock leaf zymolyte and ganoderma lucidum mushroom bran obtained in step (2), are well mixed, and obtain fermentation substrate；
(4) the above-mentioned fermentation substrate for making is put into solid-state fermenter, coefficient 0.35-0.45, it is logical in 35-45 DEG C of interval
Wind aerobic fermentation 5-8d, then stands afterripening fermentation 10-20d；
(5) after fermentation ends, fermentation materials are obtained final product through air-drying, crushing.
7. the preparation method of double source humic acid biological fertilizer as claimed in claim 6, it is characterised in that in step (1), the wind
It is 1 to change coal or peat with the weight ratio of inorganic alkali solution：1~2；Chemical activation condition is：40-50 DEG C of accumulation 3-5h；Preferably,
Described inorganic alkali solution is the KOH of mass fraction 5~15%.
8. the preparation method of double source humic acid biological fertilizer as claimed in claim 6, it is characterised in that in step (2), the hair
The water content of ferment matrix is 55-60% percentage by weights.
9. the application of double source humic acid biological fertilizer described in any one of claim 1-5, makees for fruit tree, vegetables and/or crop field
Thing, uses as base fertilizer, or topdresses and use.
10. the application of double source humic acid biological fertilizer as claimed in claim 9, wherein, the double source humic acid biological fertilizer
Consumption is as follows：
When making base fertilizer：300~500 kilograms of every mu of vegetable crop, 5~15 kilograms of every plant of fruit tree, every mu of wheat, corn or paddy rice
200~400 kilograms；
When topdressing：150~250 kilograms of every mu of vegetable crop, 5~15 kilograms of every plant of fruit tree, every mu of wheat, corn or paddy rice
100-200 kilograms.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710236114.XA CN106916021A (en) | 2017-04-12 | 2017-04-12 | A kind of double source humic acid biological fertilizer and preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710236114.XA CN106916021A (en) | 2017-04-12 | 2017-04-12 | A kind of double source humic acid biological fertilizer and preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106916021A true CN106916021A (en) | 2017-07-04 |
Family
ID=59568144
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710236114.XA Pending CN106916021A (en) | 2017-04-12 | 2017-04-12 | A kind of double source humic acid biological fertilizer and preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106916021A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109053246A (en) * | 2018-09-20 | 2018-12-21 | 鑫宏润（天津）农业生物技术服务有限公司 | A kind of agricultural wastes enzymatic isolation method continous way rapid production of organic fertilizer technique |
| CN109182163A (en) * | 2018-08-17 | 2019-01-11 | 吉林农业大学 | A kind of composite bacteria agent of efficient cryogenic degrading maize straws and its application |
| CN109704878A (en) * | 2018-06-13 | 2019-05-03 | 湖南人文科技学院 | A method of with preparing multielement organic fertilizer by spontaneous fermentation of active humic acid |
| CN110408567A (en) * | 2019-08-01 | 2019-11-05 | 山东佐田氏生物科技有限公司 | One plant of saline-alkali tolerant Methylotrophic bacillus and its active bacteria formulation and application |
| CN110818506A (en) * | 2019-11-25 | 2020-02-21 | 史丹利农业集团股份有限公司 | Special controlled release fertilizer for peanuts and preparation method thereof |
| CN113880639A (en) * | 2021-10-26 | 2022-01-04 | 淄博新农基作物科学有限公司 | Humic acid fertilizer and preparation method thereof |
| CN114009274A (en) * | 2021-12-02 | 2022-02-08 | 浙江寿仙谷医药股份有限公司 | Moisturizing synergistic ganoderma lucidum planting matrix and preparation method and efficient recycling method thereof |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1765844A (en) * | 2004-10-26 | 2006-05-03 | 刘甫名 | Humic acid biological fertilizer and its production method |
| CN101485332A (en) * | 2009-02-16 | 2009-07-22 | 王淮勇 | Multifunctional preparation and method of use thereof |
| CN101781211A (en) * | 2009-01-18 | 2010-07-21 | 陈永胜 | Method for extracting chlorogenic acid from sunflower meal |
| CN101830804A (en) * | 2010-03-16 | 2010-09-15 | 天津中新药业集团股份有限公司中新制药厂 | Method for extracting chlorogenic acid from honeysuckle by using compound enzyme method |
| CN102242100A (en) * | 2011-04-20 | 2011-11-16 | 威海康博尔生物药业有限公司 | Method for using compound biological enzyme in plant extraction process, and conditions thereof |
| CN103058772A (en) * | 2013-01-15 | 2013-04-24 | 内蒙古永业生物技术有限责任公司 | Preparation method for high-activity humic acid biofertilizer |
| CN104557337A (en) * | 2014-12-31 | 2015-04-29 | 山东海森现代农业专业合作联合社 | Orchard soil improving type organic fertilizer, as well as preparation method and application thereof |
| CN104591927A (en) * | 2015-01-27 | 2015-05-06 | 贵州大学 | Special bio-organic fertilizer for organic tea and preparation method of special bio-organic fertilizer |
| CN104628440A (en) * | 2013-11-12 | 2015-05-20 | 天津谷丰生物科技开发有限公司 | Method for preparing special vegetable organic fertilizer by using active humic acid fermentation |
| CN104987269A (en) * | 2015-08-12 | 2015-10-21 | 生之道生态农业科技股份有限公司 | Biological organic fertilizer special for apples and preparation method thereof |
| CN106316693A (en) * | 2016-08-26 | 2017-01-11 | 山东农业大学 | Biological humic acid fertilizer and preparing method thereof |
| CN106748456A (en) * | 2016-12-16 | 2017-05-31 | 郭文英 | The anti-corn stalk rot disease organic fertilizer and preparation method prepared as raw material with Chinese medicine dreg |
| CN109134081A (en) * | 2018-09-28 | 2019-01-04 | 德州佐田康瑞生物科技有限公司 | A kind of bio-feritlizer and preparation method thereof rich in functional polysaccharide |
-
2017
- 2017-04-12 CN CN201710236114.XA patent/CN106916021A/en active Pending
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1765844A (en) * | 2004-10-26 | 2006-05-03 | 刘甫名 | Humic acid biological fertilizer and its production method |
| CN101781211A (en) * | 2009-01-18 | 2010-07-21 | 陈永胜 | Method for extracting chlorogenic acid from sunflower meal |
| CN101485332A (en) * | 2009-02-16 | 2009-07-22 | 王淮勇 | Multifunctional preparation and method of use thereof |
| CN101830804A (en) * | 2010-03-16 | 2010-09-15 | 天津中新药业集团股份有限公司中新制药厂 | Method for extracting chlorogenic acid from honeysuckle by using compound enzyme method |
| CN102242100A (en) * | 2011-04-20 | 2011-11-16 | 威海康博尔生物药业有限公司 | Method for using compound biological enzyme in plant extraction process, and conditions thereof |
| CN103058772A (en) * | 2013-01-15 | 2013-04-24 | 内蒙古永业生物技术有限责任公司 | Preparation method for high-activity humic acid biofertilizer |
| CN104628440A (en) * | 2013-11-12 | 2015-05-20 | 天津谷丰生物科技开发有限公司 | Method for preparing special vegetable organic fertilizer by using active humic acid fermentation |
| CN104557337A (en) * | 2014-12-31 | 2015-04-29 | 山东海森现代农业专业合作联合社 | Orchard soil improving type organic fertilizer, as well as preparation method and application thereof |
| CN104591927A (en) * | 2015-01-27 | 2015-05-06 | 贵州大学 | Special bio-organic fertilizer for organic tea and preparation method of special bio-organic fertilizer |
| CN104987269A (en) * | 2015-08-12 | 2015-10-21 | 生之道生态农业科技股份有限公司 | Biological organic fertilizer special for apples and preparation method thereof |
| CN106316693A (en) * | 2016-08-26 | 2017-01-11 | 山东农业大学 | Biological humic acid fertilizer and preparing method thereof |
| CN106748456A (en) * | 2016-12-16 | 2017-05-31 | 郭文英 | The anti-corn stalk rot disease organic fertilizer and preparation method prepared as raw material with Chinese medicine dreg |
| CN109134081A (en) * | 2018-09-28 | 2019-01-04 | 德州佐田康瑞生物科技有限公司 | A kind of bio-feritlizer and preparation method thereof rich in functional polysaccharide |
Non-Patent Citations (1)
| Title |
|---|
| 杨继生: "《表面活性剂原理与应用》", 31 December 2012, 东南大学出版社 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109704878A (en) * | 2018-06-13 | 2019-05-03 | 湖南人文科技学院 | A method of with preparing multielement organic fertilizer by spontaneous fermentation of active humic acid |
| CN109182163A (en) * | 2018-08-17 | 2019-01-11 | 吉林农业大学 | A kind of composite bacteria agent of efficient cryogenic degrading maize straws and its application |
| CN109182163B (en) * | 2018-08-17 | 2020-10-27 | 吉林农业大学 | Composite microbial inoculum for efficiently degrading corn straws at low temperature and application thereof |
| CN109053246A (en) * | 2018-09-20 | 2018-12-21 | 鑫宏润（天津）农业生物技术服务有限公司 | A kind of agricultural wastes enzymatic isolation method continous way rapid production of organic fertilizer technique |
| CN110408567A (en) * | 2019-08-01 | 2019-11-05 | 山东佐田氏生物科技有限公司 | One plant of saline-alkali tolerant Methylotrophic bacillus and its active bacteria formulation and application |
| CN110408567B (en) * | 2019-08-01 | 2020-12-29 | 山东佐田氏生物科技有限公司 | Saline-alkali-tolerant methylotrophic bacillus and viable bacteria preparation and application thereof |
| CN110818506A (en) * | 2019-11-25 | 2020-02-21 | 史丹利农业集团股份有限公司 | Special controlled release fertilizer for peanuts and preparation method thereof |
| CN110818506B (en) * | 2019-11-25 | 2021-11-30 | 史丹利农业集团股份有限公司 | Special controlled release fertilizer for peanuts and preparation method thereof |
| CN113880639A (en) * | 2021-10-26 | 2022-01-04 | 淄博新农基作物科学有限公司 | Humic acid fertilizer and preparation method thereof |
| CN114009274A (en) * | 2021-12-02 | 2022-02-08 | 浙江寿仙谷医药股份有限公司 | Moisturizing synergistic ganoderma lucidum planting matrix and preparation method and efficient recycling method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106916021A (en) | A kind of double source humic acid biological fertilizer and preparation method and application | |
| CN103031254B (en) | Efficient straw-decomposing inoculant and preparation method thereof | |
| CN102696458B (en) | Edible fungus compost, production method thereof and edible fungus culture process | |
| CN103922818B (en) | Utilize sugar mill mud to produce technology and the technique of biological humic acid | |
| CN104446813A (en) | Bioorganic fertilizer and preparation method thereof | |
| CN103848698A (en) | Biological organic fertilizer prepared by aerobic fermentation of biogas residue and preparation method thereof | |
| CN106748341A (en) | Potato microbial bacterial manure special and preparation method thereof | |
| CN102276301B (en) | Straw decomposing agent and production method thereof | |
| CN104276874A (en) | Microorganism-enzyme powdery composite biological preparation for promoting crop yield increase | |
| CN104725111A (en) | Paddy culture medium and preparation method thereof | |
| CN104496598A (en) | Special organic fertilizer for watermelons and preparation method of special organic fertilizer | |
| CN110183266A (en) | A kind of small molecule alginic acid microbial manure | |
| CN104671860A (en) | Bioorganic fertilizer using agricultural waste as raw materials and preparation method of bioorganic fertilizer | |
| CN108658667A (en) | Ferment liquid fertilizer and its production method | |
| CN107580818B (en) | A kind of integrated approach of soil conditioning and reparation | |
| CN108017446A (en) | The preparation method of enzyme containing activated carbon and the nutritional agents of microorganism cenobium | |
| CN105601347A (en) | Preparation method for crop organic fertilizer | |
| CN104692961A (en) | Microorganism organic material decomposition agent as well as preparation method and applications thereof | |
| CN104130068A (en) | Compound multifunctional biological foliage fertilizer | |
| CN106045677A (en) | Method for preparing organic compound biological fertilizer special for fruit trees | |
| CN103396182A (en) | Method for producing solanaceous vegetable seedling growing substrate by utilizing salvaged material of cassava processing | |
| CN113135787A (en) | Rice straw decomposition agent and preparation method thereof | |
| CN113121281A (en) | Rice seedling strengthening agent and preparation method thereof | |
| CN108516896A (en) | A kind of biomass straw organic fertilizer and preparation method thereof | |
| CN107793183A (en) | A kind of biological and ecological fermentation fertilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170704 |
|
| WD01 | Invention patent application deemed withdrawn after publication |