CN108017446A - The preparation method of enzyme containing activated carbon and the nutritional agents of microorganism cenobium - Google Patents
The preparation method of enzyme containing activated carbon and the nutritional agents of microorganism cenobium Download PDFInfo
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- CN108017446A CN108017446A CN201711274538.1A CN201711274538A CN108017446A CN 108017446 A CN108017446 A CN 108017446A CN 201711274538 A CN201711274538 A CN 201711274538A CN 108017446 A CN108017446 A CN 108017446A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The present invention relates to the preparation method of a kind of enzyme containing activated carbon and the nutritional agents of microorganism cenobium, the fermentation raw material and proportion：Mushroom mushroom bran mass percent 85%, wheat bran mass percent 10%, corn flour mass percent 5%, fermentation raw material adds water after crushing make fermentation raw material moisture content mass percent be 25~35%, and adding composite bacteria agent by 1: 1000 mass ratio is uniformly mixed enzyme containing activated carbon and the nutritional agents of microorganism cenobium.The present invention make composite flora overcome colonized in ecological environment of soil during competed with other microbiologic populations so as to forming dominant population, there are enough Populations to overcome the repulsion of the soil habitat microorganism including pathogen to keep it to be colonized around plant roots, leaf, secrete various enzymes and antibiotic, the effect such as induction disease resistance of plant, play the advantage that biological pesticide killing pests and suppressing bacteria speed is fast, prevention effect is stablized and achievees the purpose that mutual supplement with each other's advantages, dosage are reduced, preventive effect strengthens by compounding synergistic effect.
Description
Technical field
The present invention relates to the preparation method of a kind of enzyme containing activated carbon and the nutritional agents of microorganism cenobium.
Background technology
With the high speed development of chemical industry, the production of various chemical fertilizer and amount of application are continuously increased, to agricultural production
Development play important impetus, for solve China food problem made distinctions won on the battlefield.But as chemical fertilizer is applied
Dosage is continuously increased, it is also gradually exposed using shortcoming, and analysis gets up to be mainly manifested in the following aspects：
1st, fertilizer efficiency utilization rate is low.
2nd, environmentally protective difference, causes soil hardening serious, deteriorates the physicochemical property of soil, blocks soil aperture, destroys soil
Earth crumb structure, causes unbalance soil nutrient elements, hardened, pollution and fertility to decline, and soil-borne disease getting worse.
3rd, heavy-metal residual, crop quality is low, directly threatens human life's safety.
For above-mentioned drawback, be sought after one kind in cultivation of fields, can activating soil, reduce the usage amount of chemical fertilizer, reduce pair
The pollution of environment.Strengthen crops to absorb conditioning agent, overcome current common micro-organisms fertilizer, function is single, effect effect
Fruit unobvious shortcoming, controls disease theoretical, the complementation of different biocontrol microorganisms, is ground by deep experiment according to bio-diversity
Study carefully and production practices repeatedly.Obtain important breakthrough, with reference to agrophysical technology, agrology, microbiology, biochemistry into
Microbial biocontrol, biological enzyme technology, agriculture waste produce CO 2 technology, biochemistry prevention are combined and answered work(
For agricultural production, mend carbon activity carbon and cure, microorganism improvement soil, carbon-based organic fertilizer quick transformation soil texture, balances salt
With moisture, the soil environment for being conducive to plant health and growing is created by rapid aging, so as to increase soil fertility, promotes crop
Growth, starts green agriculture new era.
The soil organism is that carbon important in one of important indicator of soil fertility, and terrestrial ecosystems converges.Biology
Charcoal phosphorus content is high, and carbon structure is in aromatic ring-shaped, and similar to Soil Humus structure, research both at home and abroad shows that charcoal can
To improve soil organic carbon level, there is farming area in Zuogong soil effect.Bruun etc. is using C14 mark charcoals and crop
2 years soil incubations experiment of stalk finds that the carbon loss of low temperature and high-temperature biological charcoal is respectively 9.3%, 3.1%, well below
The carbon loss of stalk (56%).Kimetu etc. also reports, the carbon loss of charcoal is far below green manure, the stability of charcoal and steady
Determining effect is more than green manure class legibility organic matter.Stalk, green manure, compost and barnyard manure are manured into soil and under no-tillages, generally
Remaining only 20% after 5-10, only very small amount is converted into humus, and Britain's Lausanne experiment centre applies barnyard manure more than 150 years
Soil organic matter amount also improve only 1 times.And the research of Amazon basin black earth (Terra Preta) soil quality shows, biology
Charcoal is more stable more than traditional organic material, is more easy to improve the organic material of soil organic matter, therefore the United Nations's chemistry pollution prevention is public
About and some governments appeal using charcoal as soil improvement and reply climate change important technology product.
In addition, increasing carbon dioxide to crop, the photosynthesis of crop, the yield of increase crop, improvement can be promoted
The quality of product.The compression that can be directly filled as the carbon dioxide that fertilizer is applied into greenhouse with dry ice or steel cylinder
Carbon dioxide discharges, and can also be obtained from the flue gas of burning carbonaceous material, but most convenient, most cheap, most common method are
Directly produced using strong acid and reaction containing ammonium bicarbonate soln.The compression carbon dioxide filled with dry ice or steel cylinder discharges titanium dioxide
Carbon cost is higher, is difficult to purify containing carbon monoxide in the carbon dioxide obtained from combustion product gases, with strong acid and ammonium hydrogen carbonate
Carbon dioxide is produced in reaction, although of low cost, gas purity is high, since strongly acidic solution has very strong corrosivity, is transported
It is defeated, storage is very inconvenient, and the reaction speed of strongly acidic solution and ammonium hydrogen carbonate is exceedingly fast, and is difficult to the generation speed of carbon dioxide
Control, or even can hurt sb.'s feelings because of liquid splash.Chinese patent ZL200410024965.0 and Chinese patent
ZL200710068728.8 disclose it is a kind of using agricultural organic waste ferment carry out applying CO 2 fertilizer in greenhouse method and
Device.This method is using agricultural crop straw and feces of livestock and poultry mixture as matrix, and the C/N ratios for making matrix are 40/1, and heap is filled in fermentation
In putting, directly ferment in greenhouse, fermentation process keeps fermentation temperature at 50 DEG C or so, matrix pH value 6.0~7, in greenhouse
Carbon-dioxide gasfertilizer is discharged, carbon-dioxide gasfertilizer concentration in greenhouse can be made to maintain 800ul/L.But find in the application, the party
Method in the case where canopy indoor temperature is relatively low, it is necessary to can be only achieved within 4-5 days 50 DEG C of fermentation temperature, and installation for fermenting design comparison
Complexity, cost is higher, is still to use sulfuric acid in gas absorbent assembly, peasant household is not easy purchase and dangerous property, have impact on the hair
Bright popularization and application.
At present, agriculture seedbed nutritional agents patent of invention technical solution is single, and microorganism species advantage unobvious, do not accomplish
The combination of physical method, biochemistry and microorganism, biocontrol effect unobvious cannot have complementary advantages, and be agriculture currently on the market
The people will cure the disease just to be compounded with various products, and expense is high.So it is badly in need of a kind of full-featured, application effect on agricultural materials market
Obvious product.
The content of the invention：
The object of the present invention is to provide a kind of conditioning agent content of organic matter height of production, raising ground temperature, crop can be improved
Absorption to phosphorus, potassium simultaneously has anti-soil-borne disease, Soluble phosphorus potassium decomposing fixed nitrogen, increases carbon dioxide effect, is environmentally protective containing activity
The preparation method of the seedbed nutritional agents of carbon enzyme and microbial bacterial agent.The technical scheme is that：A kind of enzyme containing activated carbon and micro-
The preparation method of the nutritional agents of biological cenobium, comprises the following steps：
(1) fermentation raw material is edible fungus culturing residue material, the fermentation raw material and proportion：Mushroom mushroom bran matter
Percentage 85% is measured, wheat bran mass percent 10%, corn flour mass percent 5%, water is added after crushing contains fermentation raw material
Water rate mass percent is 25~35%, and adding composite bacteria agent by 1: 1000 mass ratio is uniformly mixed, and the composite bacteria agent is：
Bacillus pumilus Bacillus pumilus (bacterium number：ACCC10113), thermophilic saline and alkaline bacillus
Bacillushaloalkaliphilus (ACCC10232) blown-ball Azotobacter Azotobacter chroococcum (bacterium number
), ACCC11104 bacillus laterosporus Bacillus laterosporus Laubach (bacterium number：ACCC11079), the false silk in the torrid zone
Yeast Candida tropicalis bacterium number：ACCC20275), S. cervisiae Saccharomyces cerevisiae
Hansen (bacterium number：ACCC20107), Rhodopseudomonas palustris Rhodop seudanonas palustris (bacterium number：
ACCC10149), bacillus subtilis Bacillus subtilis (bacterium number：ACCC10632), bacillus amyloliquefaciens
Bacillus amyloliquefaciens (bacterium number：ACCC10225), bacillus polymyxa Paenibacillus polymyxa
(bacterium number：ACCC1010122), bacillus licheniformis Baclicuslincheniformis (bacterium number：ACCC11080), fluorescence is false
Pseudomonas bacillus Pseudomonasfluorescens (ACCC10040).Visit and carry out mould penicillium bilii (bacterium number：
ACCC30440), penicillium oxalicum Penicillium oxalicum (bacterium number：ACCC30400), sporotrichum thermophile
Sporotrichum thermophile (bacterium number：ACCC30346), aspergillus niger Aspergillus niger (ACCC30557),
Trichoderma harzianum Trichoderma harzianum (bacterium number：ACCC30371), trichoderma reesei Trichoderma reesei (bacterium
Number：ACCC30449 composition), its form strain provide commercially available solid for Chinese agriculture Microbiological Culture Collection administrative center
Body bacterium powder, effective bacterium number bacterium are 2 × 1010More than cfug-1's, bacillus pumilus in the composition：Thermophilic saline and alkaline gemma bar
Bacterium：Blown-ball Azotobacter：Bacillus laterosporus：Candida tropicalis：S. cervisiae：Rhodopseudomonas palustris：Bacillus subtilis
Bacterium：Bacillus amyloliquefaciens：Bacillus polymyxa：Bacillus licheniformis：Pseudomonas fluorescence：Visit and carry out mould：Penicillium oxalicum：
Sporotrichum thermophile：Aspergillus niger：Trichoderma harzianum：Trichoderma reesei：Each material mass ratio is 1: 1 in composition；Keep temperature for 55~
65 DEG C, ferment 2 days, fermentation ends material water ratio is 25~35%；Contain in enzyme containing activated carbon and the nutritional agents of microorganism cenobium
Have 2 × 108Effective bacterium number bacterium of more than cfug-1, it is spare；
(2), the mixed raw material fermented of step (1) and composite bacteria agent is added into the activity that mass percent is 20%
Carbon obtains finished product；
(3), the compound biological enzyme of step (2) finished product gross mass 1% is added；
(4), step (3) finished product and compound biological enzyme are carried out in bulk after mixing or are granulated, dry, moisture content is matter
Percentage 10% is measured, packaging, is made activated carbon enzyme microbial manure.
The activated carbon is stalk, wooden made mechanism powdered carbon.
The complex enzyme that the compound biological enzyme compounds for two or more following any biology enzymes, including：Neutral metal egg
White enzyme, alkaline phosphatase：Carbohydrase, amylase, acid protease, pectase, glucose oxidase, catalase, ribose
Nuclease, lipase, cellulase, hesperidinase, naringinase.
Length≤1mm after the agricultural organic waste crushing.
The technology of the present invention effect
Control disease theoretical according to bio-diversity, the complementation of different microorganisms flora, is ground by deep experiment
Study carefully and production practices repeatedly.Successfully microorganism is given birth to reference to agrophysical technology, agrology, microbiology, biochemistry
Thing prevention, biological enzyme technology, agriculture waste produce CO 2 technology, biochemistry prevention are combined applied to agricultural production,
Mend carbon activity carbon to cure, microorganism improvement soil, carbon-based organic fertilizer quick transformation soil texture, balances salt and moisture, by fast
The soil environment for being conducive to plant health growth is created in speed curing, so as to increase soil fertility, is promoted plant growth, is started green
Agriculture new era.
Carbon-based fertilizer improves crop quality with adsorbing and being passivated heavy metal-polluted soil and pesticide residue.
The soil organism is that carbon important in one of important indicator of soil fertility, and terrestrial ecosystems converges.Biology
Charcoal phosphorus content is high, and carbon structure is in aromatic ring-shaped, and similar to Soil Humus structure, research both at home and abroad shows that charcoal can
To improve soil organic carbon level, there is farming area in Zuogong soil effect.Bu Luen (Bruun) etc. is using C14 mark biologies
The experiment of 2 years soil incubations of charcoal and crop material finds that the carbon loss of low temperature and high-temperature biological charcoal is respectively 9.3%, 3.1%,
Well below the carbon loss of stalk 56%.He Xusheng etc. also reports that the carbon loss of charcoal is far below green manure, the stabilization of charcoal
Property and static stabilization are more than green manure class legibility organic matter.Stalk, green manure, compost and barnyard manure are manured into soil and in no-tillages
Under, remaining only 20% after general 5-10, only very small amount is converted into humus, and Britain's Lausanne experiment centre applies barnyard manure
The soil organic matter amount of more than 150 years also improve only 1 times.And Amazon basin black earth (Terra Preta) soil quality is studied
Show, charcoal is more stable more than traditional organic material, is more easy to improve the organic material of soil organic matter, therefore the United Nations prevents
The important technology product of climate change is appealed using charcoal as soil improvement and tackled in desertification pact and some governments.
Carbon-based fertilizer nutrient can slowly be decomposed by microorganism, and nutritional ingredient can be put aside in the soil, be not easily runed off,
Humus after organic fertilizer decomposes is combined with grogs, is formed crumb structure, is added the permeability of soil, can strengthen crops pair
Carbon, hydrogen, oxygen absorb, and are conducive to chesson.The disease resistance of plant can be improved by increasing carbon-based fertilizer, mitigate plant disease
Generation.And contain a large amount of benefit materials, including：A variety of organic acids, peptides and the abundant battalion including nitrogen, phosphorus, potassium
Support element.Full nutrition can not only be provided for crops, and fertilizer efficiency is grown, and can be increased and be updated the soil organism, promote micro- life
Thing is bred, and improves the physicochemical property and bioactivity of soil, is the Main Nutrients of green and organic foods production.
Since disorderly to fertilize, soil becomes due to a lack of organic matter causes soil fertility to decline to a great extent and hinders China's agricultural can
An important factor for sustainable development, therefore country is wideling popularize application of organic fertilizers, especially carbon-based fertilizer！
Enzyme is also referred to as ferment, is the polymer substance with life catalytic action, contains their branch of thousands of enzymes in organism
Match somebody with somebody biologyMetabolism, nutrition and energy conversion etc. many catalytic process, reaction in close relations is most with life process
It is enzymic catalytic reaction, does not just have life without enzyme.Organism byCellForm, each cell due toEnzymePresence just show to plant
KindLifeActivity, internalMetabolismIt could carry out.Enzyme is metabolic in organismCatalyst, onlyEnzymeIn the presence of raw
Items could be carried out in objectBiochemical reaction.Enzyme is more in organism, more complete, its life is more healthy.OrganismDisease,
It is most of with azymia or synthesisObstacleIt is related.
The chemical nature of enzyme is protein, so the process of its synthesis is similar with the synthesis of protein.In the cell, enzyme
Synthesis also withNutrientIt is in close relations with metabolite.CertainNutrientIntake, particularly some trace element take the photograph
Enter, the synthesis of corresponding enzyme can be promoted.Thus enzyme and middle trace element are complementary.Enzyme can be divided into metal from form
Protease and nonmetallic protease, metalloproteinases are middle micro- highest morphology in itself, can promote the further of enzyme
There is catalysis, adjusting, reduction etc. to act on for synthesis and generation, nonmetallic protease.
Chinese agricultural development outlet is yeast-like fungi element, yeast-like fungi element mainly containing various beneficial bacteriums and various enzymes and in micro member
Element, the effect of biological bacteria also mainly produce various enzymes, promote crop growth, improve nutrien utilization, and suppress germ production
It is raw.Thus enzyme is Source of life, is the essential living matter of agricultural sustainable development.
Metalloprotease, is albumen state metal ion.Middle Forms of microelements is divided into four developing stage, lowermost level
For oxide, such as various ore-iron oxide-cupric oxide, these material water can not dissolve, so plant can not also absorb
Utilize, can only be mineral matter, it is impossible to be known as nutrient.
Second stage is metallic salt, for example zinc sulfate, calcium carbonate etc., these metallic salts can be dissolved in water, so
It can be absorbed and used by plants, but shortcoming is to use easily to be solidified into water by other metal ions in soil and do not dissolve, and is known as crop
Nonabsorbable nutrient.This kind of most suitable foliage fertilization of trace element.
Three phases are micro- in organic chelated class, be in salt technology by organic chelated, it is micro in
Metal element ion and the external world separate, and cure so as to avoid by other metal ions, so as to improve utilization rate.But this kind of production
Product, complex production process, the bad control of technical indicator, cost are also higher.
Four-stage is trace element in albumen state, also referred to as metalloprotein.Crop is usually micro in absorption salt
Element, by series reaction, produces amino acid and protein, a portion protein is metalloprotein, and usual crop will
1 ton of metallic salt of consumption could produce 10 grams of metalloprotein, and this process consumes a part of plant nutrient and energy.To be assorted
It is highest morphology to say metalloprotein, because on the one hand it is absorbed, saves crop and absorbs manufacture nutrient process, a side
Face reduces crop nutrition content consumption.MetalProteaseIt can directly be absorbed by plant, therefore plant can be saved and convertedMicro member ElementIn required " physical efficiency ", greatly promote growth and development of plants.Through experiment, using polypeptide fertilizer, plant can generally shift to an earlier date 5-
15d maturations (Corn carriesPreceding 5d or so, cotton shift to an earlier date 7-10d, and tomato shifts to an earlier date mono- 15d of lO), and chemical fertilizer utilization ratio can be improved
With crops quality etc..Such as insulin is also zinc albuminate, so it is also fertilizer that metalloprotein, which is enzyme,.Crop is set to be carried in Nutrient Absorption
Outside height, increase can disease resistance and antioxidant and anti-aging ability.
Enzyme control is exactly that crop is controlled to Nutrient Absorption by enzyme, if the growth busy season, crop is strengthened inhaling nutrient naturally
Receive and utilize, what nutrition lacked, additional enzyme will promote what nutrient uptake and utilization crop strengthens.So as to improve fertilizer nutrient profit
With rate, crop is promoted to absorb.
The present invention is using agricultural organic waste edible fungus bran as a kind of the main of microbial manure of enzyme containing activated carbon
Raw material, using biotechnology, physical technique, enzymology technology is combined, and has not only accomplished waste utilization, alleviates environment dirt
Dye, and the conditioning agent content of organic matter produced is high, can drastically increase the ADSORPTION STATE in phosphorus in discarded object, potassium and soil
The utilization rate of phosphorus, potassium, while microorganism can produce the work that organic acid, polysaccharide etc. are conducive to plant absorption during growth and breeding
Chemical conversion point, can improve soil soil hardening caused by chronic administration chemical fertilizer, adjust the crumb structure of soil, increase soil
Permeability, is conducive to crop root growth and the absorption to nutrient, improves the fertilizer-preserving ability and cushion performance of soil, contains
Humus there is physiological activity, crop growth can be promoted, contain the edible fungi polysaccharide in edible fungus bran in conditioning agent
There is good prophylactic-therapeutic effect to the viroses of plant, reduce disease.The dissolving phosphor and dissolving potassium bacterium contained can be by Silicon Valley hydrochlorate and aluminium silicon
The active ingredient such as hydrochlorate and phosphorus in other ores, potassium, which decomposites, to be come, and has the function of that Soluble phosphorus, Potassium release are prevented and cured diseases, fungus treatment
Function, the generation of controlling plant diseases can improve the survival rate of plant, and shorten the maturity period, can freshness date extend 5-10 times.
The flora that the present invention contains isBacillus pumilusEnergy hydrolysis starch, degradedMannosan, xylan, cellulose etc.
Prevent root rotof flax, grey mould fruit rot of strawberry.Blown-ball Azotobacter has biological nitrogen fixation ability, is provided for plant particularly cereal crops
Nitrogen, improve yield, reduce fertilizer amount and production cost, establish the ecological balance and promote agricultural sustainable development with important
Meaning.Visit and carry out mould, Soluble phosphorus penicillium oxalicum has phosphorus decomposing and prophylaxis effect, and in terms of phosphorus decomposing, the phosphorus used with the existing country is thin
Bacterium is compared, its phosphorus decomposing effect stability；In terms of diseases prevention, compared with the phosphobacteria that the existing country uses, its diseases prevention for having uniqueness is made
With.Effect is significantly inhibited to a variety of important phytopathogens.Biological and ecological methods to prevent plant disease, pests, and erosion flora is side bacillus, bacillus subtilis, solution
Bacillus amyloliquefaciens, bacillus polymyxa, Trichoderma harzianum, Trichoderma viride, by nutrition site competition, secretion resisting substance, lure
The mechanism such as crop resistance are led, soil-borne fungus is resisted and crop is infected.In addition the active matter for promoting plant growth can also be secreted
Matter, makes plant well established and vigorously developing, plays the role of disease-resistant yield-increasing.With efficient prevention various crop pest and disease damage, crop immunity is improved,
Promote crop root development, increasing yield and improving quality, environmentally protective multiple action.
Photosynthetic bacteria Rhodopseudomonas palustris has the function of the disease-resistant toadstool of fixed nitrogen, and photosynthetic bacteria is a kind of nitrogen-fixing bacteria again,
The nitrogen that can be fixed in air is translated into the nitrogen of crop needs, and nitrogen-fixing bacteria in soil Barren are administered simultaneously after photosynthetic bacteria
Quantity, which increases, further increases the effect of fixed nitrogen.Photosynthetic bacteria is a kind of growth-promoting root fungus again, using can increase crop after photosynthetic bacteria
The length of root and the quantity of Gen Mao, greatly reinforce the effect that root absorbs nutrient, this is that it can promote the another of crop yield
Kind important mechanisms.In addition it is known that some plant hormones will be added in present commercial fertilizer to improve the effect of fertilizer
Fruit, and photosynthetic bacteria can inherently be metabolized it is a variety of have remarkably promote plant growth effect important substance such as proline, urine it is phonetic
Pyridine, cytimidine, vitamin, ubiquinone, carotenoid etc. can directly be absorbed by crop, while more importantly can be significantly
The quality of crop and fruit is improved, considerably beyond the effect for applying plant hormone class.Photosynthetic bacteria by nitrate reductase and
Nitrite reductase nitrate reduction into amino acid or ammonia so as to greatly reduce the content of nitrite in fruits and vegetables grain,
The probiotics such as the favourable bacillus for suppressing pathogen again at the same time of Crops growth, increasing production disease-resistant effect can be more preferable.
Using the Expectancy that can mitigate crop after Efficiency of Photosynthetic Bacterial Manure significantly.Reason for that is as follows：Has Chinese Academy of Sciences's application ecology
Research institute reports, and has three classes bacterium in the Rhizosphere Soil Barren of crop, and one kind is to promote plant growth while and disease prevention work of having
" growth-promoting rhizosphere bacterium ", which is referred to as, beneficial to mushroom accounts for total 2% -5% of whole rhizosphere microorganism；Another kind of is that harmful bacteria accounts for
The 8%-15% of rhizosphere microorganism sum；Remaining accounts for the unhelpful harmless bacterium of category of 80%-90%.Growth-promoting root mark bacterium therein according to
The root system of harmful bacteria infringement crop is prevented by secretion antibiotic, while secrete various somatotropic hormones usually to stimulate the life of crop
It is long.They to cover crop root system and increase yield play the role of it is vital, when growth-promoting rhizosphere bacterium quantity reduce when
Disease and the underproduction will occur for crop.And it is exactly to make when crop applies most important effect caused by Efficiency of Photosynthetic Bacterial Manure
The quantity of growth-promoting rhizosphere bacterium greatly increases, and the various antibiotic that it is secreted after the quantity increase of actinomyces are (at present agriculturally
The various antibiotic product overwhelming majority for sterilization are as caused by actinomyces) play the role of suppressing pathogen,
It can also induce at the same time and improve the disease resistance of crop in itself.
The flora that soil organic matter degraded heats production carbon dioxide is sporotrichum thermophile, candida tropicalis, yeast flora,
A large amount of mycoproteins and abundant vitamin B complex are produced at the same time.Therefore, the generation of aerogen flora is loaded into fermentation substrate
Carbon-dioxide gasfertilizer, rise in value antibiosis flora, becomes new Agricultural Practical Techniques.Microorganism in the microbial fermentation strain of addition
It is thermophilic and active stronger microorganism, some more complicated organic matters can be promptly started with, such as pectin, fat
Deng.They can dramatically increase the micro organism quantity in fermentation substrate in a short time, optimize microbiological paper method therein,
Promote the degraded of degradable thing in fermentation substrate, accelerate the heating of fermentation substrate.Due to microbial activities amount in fermentation substrate
Dramatically increase, be greatly improved the programming rate and fermentation persistence of fermentation substrate so that fermentation substrate programming rate is fast, aerogenesis
It is efficient, and start to discharge great amount of carbon dioxide, up to more than 20 days, the quantity of biological and ecological methods to prevent plant disease, pests, and erosion flora sharply increased, and biocontrol effect is notable.
In conclusion the present invention mainly cures biological bacteria and enzyme using with activated carbon, mend carbon and mend enzyme and non-antagonist with other
Microorganism cooperates with the measure of diseases prevention.Make composite flora overcome colonized in ecological environment of soil during with other micropopulations
Fall competition and be allowed to enough Population so as to forming dominant population to overcome the micro- life of soil habitat including pathogen
The effects such as the repulsion of thing keeps it to be colonized around plant roots, leaf, secretes various enzymes and antibiotic, induction disease resistance of plant are at the same time
The advantage that performance biological pesticide killing pests and suppressing bacteria speed is fast, prevention effect is stablized reaches mutual supplement with each other's advantages by compounding synergistic effect, uses
Amount is reduced, the purpose of preventive effect enhancing.It is the two or more antagonistic microbes that will mutually melt that interaction is cooperateed with other antagonistic microbes
There is no the mixing of the mutex relations such as antagonism, parasitism, dissolving or competition to be beneficial to hybrid bacterial strain pair between mixed different antibiotic bacterias
The space of plant different parts capture comprehensively due to mix bacterium agent adapt to different environmental conditions can the light of bigger, temperature, gas,
In humidity luffing existence procreation be therefore expected to realize a variety of antibiotic bacterias have complementary functions, multiple diseases and the anti-, collaboration of persistent
Controlling effect, biocontrol microorganisms can be mixed and made into biological prevention and control agent with other function bacteriums to use.Activated carbon enzyme microbial manure substratess
Middle addition bacillus pumilus, thermophilic saline and alkaline bacillus, blown-ball Azotobacter, bacillus laterosporus, candida tropicalis, wine brewing ferment
It is female bacterium, Rhodopseudomonas palustris, bacillus subtilis, bacillus amyloliquefaciens, bacillus polymyxa, bacillus licheniformis, glimmering
Light Pseudomonas alba, visit and carry out the beneficial bacteriums such as mould, penicillium oxalicum, sporotrichum thermophile, aspergillus niger, Trichoderma harzianum, trichoderma reesei
Biocontrol microorganisms, compound biological enzyme and play mend carbon solidification activated carbon, thus it both containing needed for plant growth it is organic into
It is a kind of having for multi-functional controlling plant diseases that dividing again, there is prophyiaxis and promoting growth, which to act on and can improve soil physico-chemical property,
Machine carbon biological flora nutritional agents.
Charcoal can load fertilizer nutrient ion by Electrostatic Absorption.Secondly, its chemical functional group is more active, such as carboxylic
Base, hydroxyl, can be chemically reacted, therefore charcoal can load specific fertilizer nutrient by chemically reacting.Finally, charcoal can
To close storage and adsorption sticking fertilizer nutrient by hole.Significantly extend Nitrogen supplying, increase Soil Nitrogen total nitrogen, available phosphorus contain
Amount, promotes rice leaf phosphorus and potassium to absorb, and charcoal dosage height significantly improves rice leaf nitrogen.And potassium Leaching loss increase with
Charcoal is related rich in potassium, and it is due to that plant growth amount increases and increases potassium absorption and lead that available potassium in soils, which compares control,
Cause.It significantly reduces wash out amount in urea soil to charcoal, and nitrogen utilization efficiency improves 10%~25%.Using charcoal in fertilizer
In absorption method prepare charcoal based compound fertilizer, significantly delay fertilizer nutrient Static Water, the soil liquid elution under release.
Good nutrient slowly-released performance is presented in these fertilizer.Charcoal not only can load slow release chemical fertilizer nutrient, also can be used as biology
The carrier of microbial inoculum fertilizer.Biological carbon surface and hole can load inoculation bacterium, and bamboo charcoal and charcoal are inoculated with agent carrier as bio-bacterial manure
It has been ripe technology.Charcoal has similar physicochemical characteristics with bamboo charcoal and charcoal, therefore charcoal can also be used as bio-feritlizer
The carrier of Inoculant (such as rhizobium, phosphorus-solubilizing bacteria), supports its growth and release in the soil.Charcoal is conducive to be loaded
The spore germination of fungi and to parasitic plant element absorb.Charcoal and fertilizer mix apply significantly improve wheat, soybean and, sorghum and water
The yield of rice, corn and peanut, sorghum etc..Charcoal and fertilizer, which mix, to be applied or the notable fertilizer efficiency of composite application and charcoal and fertilizer
Complementation or synergistic effect it is related because charcoal extend fertilizer nutrient acquisition time, reduce nutrient loss, otherwise fertilizer eliminate
The defects of charcoal nutrient is insufficient.But after fertilising material and charcoal are mixed in lime soil, soybean biomass increase, wheat
Reduced with radish biomass, this may be related with crop nutritive peculiarity difference.Charcoal Mechanism of fertilizer efficiency charcoal contains containing mineral nutrient
Measure low, its direct nutrient effect is limited.On most of soil, charcoal to the facilitation of plant growth and yield very
Few directly nutrient is related, mostly with its improved indirectly-acting to soil physics, chemical property and microbial activity and
It is related to reducing the effect of soil and fertilizer leaching loss of nutrient.Soil pH influences soil nutrient availability, and soil nutrient availability changes
Then influence plant growth and yield.Influence of the charcoal to the pH of acid soil is obvious.Charcoal (gasification rice hull carbon 600
DEG C) promote the corn emergence rate of experiment soil and root system amount to increase.
Embodiment：
A kind of preparation method of enzyme containing activated carbon and the nutritional agents of microorganism cenobium：(1) fermentation raw material is edible fungus culturing
Residue material, the fermentation raw material and proportion：Mushroom mushroom bran mass percent 85%, wheat bran mass percent
10%, corn flour mass percent 5%, water is added after crushing makes fermentation raw material moisture content mass percent be 25~35%, by 1
: 1000 mass ratio adds composite bacteria agent and is uniformly mixed, and the composite bacteria agent is：Bacillus pumilus Bacillus pumilus
(bacterium number：ACCC10113), thermophilic saline and alkaline bacillus haloalkaliphilus (ACCC10232) blown-ball Azotobacter
Azotobacter chroococcum (bacterium ACCC11104), bacillus laterosporus Bacillus laterosporus
Laubach (bacterium number：ACCC11079), candida tropicalis Candida tropicalis bacterium number：ACCC20275), wine brewing ferment
Female bacterium Saccharomyces cerevisiae Hansen (bacterium number：ACCC20107), Rhodopseudomonas palustris Rhodop
Seudanonas palustris (bacterium number：ACCC10149), bacillus subtilis Bacillus subtilis (bacterium number：
ACCC10632), bacillus amyloliquefaciens Bacillus amyloliquefaciens (bacterium number：ACCC10225), more viscous gemma
Bacillus Paenibacillus polymyxa (bacterium number：ACCC1010122), bacillus licheniformis
Baclicuslincheniformis (bacterium number：ACCC11080), Pseudomonas fluorescence Pseudomonasfluorescens
(ACCC10040).Visit and carry out mould penicillium bilii (bacterium number：ACCC30440), penicillium oxalicum Penicillium
Oxalicum (bacterium number：ACCC30400), sporotrichum thermophile Sporotrichum thermophile (bacterium number：ACCC30346)、
Aspergillus niger Aspergillus niger (ACCC30557), Trichoderma harzianum Trichoderma harzianum (bacterium number：
ACCC30371), trichoderma reesei Trichoderma reesei (bacterium number：ACCC30449 composition), during its form strain is
The commercial solid bacterium powder that agricultural Microbiological Culture Collection administrative center of state provides, effective bacterium number bacterium are 2 × 1010More than cfug-1
, bacillus pumilus in the composition：Thermophilic saline and alkaline bacillus：Blown-ball Azotobacter：Bacillus laterosporus：Tropical vacation silk ferment
It is female：S. cervisiae：Rhodopseudomonas palustris：Bacillus subtilis：Bacillus amyloliquefaciens：Bacillus polymyxa：Lichens bud
Spore bacillus：Pseudomonas fluorescence：Visit and carry out mould：Penicillium oxalicum：Sporotrichum thermophile：Aspergillus niger：Trichoderma harzianum：Trichoderma reesei：
Each material mass ratio is 1: 1 in composition；It is 55~65 DEG C to keep temperature, is fermented 2 days, fermentation ends material water ratio for 25~
35%；Contain 2 × 10 in enzyme containing activated carbon and the nutritional agents of microorganism cenobium8Effective bacterium number bacterium of more than cfug-1, it is spare；
(2), the mixed raw material fermented of step (1) and composite bacteria agent is added into the activity that mass percent is 20%
Carbon obtains finished product；
(3), the compound biological enzyme of step (2) finished product gross mass 1% is added；
(4), step (3) finished product and compound biological enzyme are carried out in bulk after mixing or are granulated, dry, moisture content is matter
Percentage 10% is measured, packaging, is made activated carbon enzyme microbial manure.
The activated carbon is stalk, wooden made mechanism powdered carbon.
The complex enzyme that the compound biological enzyme compounds for two or more following any biology enzymes, including：Neutral metal egg
White enzyme, alkaline phosphatase：Carbohydrase, amylase, acid protease, pectase, glucose oxidase, catalase, ribose
Nuclease, lipase, cellulase, hesperidinase, naringinase.
Length≤1mm after the agricultural organic waste crushing.
Claims (4)
1. the preparation method of a kind of enzyme containing activated carbon and the nutritional agents of microorganism cenobium, it is characterized in that：Comprise the following steps：
(1) fermentation raw material is edible fungus culturing residue material, the fermentation raw material and proportion：Mushroom mushroom bran quality hundred
Divide ratio 85%, wheat bran mass percent 10%, corn flour mass percent 5%, water is added after crushing makes fermentation raw material moisture content
Mass percent is 25~35%, and adding composite bacteria agent by 1: 1000 mass ratio is uniformly mixed, and the composite bacteria agent is：It is short and small
Bacillus pumilus (bacterium number：ACCC10113), thermophilic saline and alkaline bacillus
Bacillushaloalkaliphilus (ACCC10232) blown-ball Azotobacter Azotobacter chroococcum (bacterium number
), ACCC11104 bacillus laterosporus Bacillus laterosporus Laubach (bacterium number：ACCC11079), the false silk in the torrid zone
Yeast Candida tropicalis bacterium number：ACCC20275), S. cervisiae Saccharomyces cerevisiae
Hansen (bacterium number：ACCC20107), Rhodopseudomonas palustris Rhodop seudanonas palustris (bacterium number：
ACCC10149), bacillus subtilis Bacillus subtilis (bacterium number：ACCC10632), bacillus amyloliquefaciens
Bacillus amyloliquefaciens (bacterium number：ACCC10225), bacillus polymyxa Paenibacillus polymyxa
(bacterium number：ACCC1010122), bacillus licheniformis Baclicuslincheniformis (bacterium number：ACCC11080), fluorescence is false
Pseudomonas bacillus Pseudomonasfluorescens (ACCC10040).Visit and carry out mould penicillium bilii (bacterium number：
ACCC30440), penicillium oxalicum Penicillium oxalicum (bacterium number：ACCC30400), sporotrichum thermophile
Sporotrichum thermophile (bacterium number：ACCC30346), aspergillus niger Aspergillus niger (ACCC30557),
Trichoderma harzianum Trichoderma harzianum (bacterium number：ACCC30371), trichoderma reesei Trichoderma reesei (bacterium
Number：ACCC30449 composition), its form strain provide commercially available solid for Chinese agriculture Microbiological Culture Collection administrative center
Body bacterium powder, effective bacterium number bacterium are 2 × 1010More than cfug-1's, bacillus pumilus in the composition：Thermophilic saline and alkaline gemma bar
Bacterium：Blown-ball Azotobacter：Bacillus laterosporus：Candida tropicalis：S. cervisiae：Rhodopseudomonas palustris：Bacillus subtilis
Bacterium：Bacillus amyloliquefaciens：Bacillus polymyxa：Bacillus licheniformis：Pseudomonas fluorescence：Visit and carry out mould：Penicillium oxalicum：
Sporotrichum thermophile：Aspergillus niger：Trichoderma harzianum：Trichoderma reesei：Each material mass ratio is 1: 1 in composition；Keep temperature for 55~
65 DEG C, ferment 2 days, fermentation ends material water ratio is 25~35%；Contain in enzyme containing activated carbon and the nutritional agents of microorganism cenobium
Have 2 × 108Effective bacterium number bacterium of more than cfug-1, it is spare；
(2), step (1) is added the activated carbon that mass percent is 20% with the composite bacteria agent mixed raw material fermented to obtain
To finished product；
(3), the compound biological enzyme of step (2) finished product gross mass 1% is added；
(4), step (3) finished product and compound biological enzyme are carried out in bulk after mixing or are granulated, dry, moisture content is quality hundred
Divide ratio 10%, packaging, is made activated carbon enzyme microbial manure.
2. the preparation method of a kind of enzyme containing activated carbon according to claim 1 and the nutritional agents of microorganism cenobium, its feature
It is：The activated carbon is stalk, wooden made mechanism powdered carbon.
3. the preparation method of a kind of enzyme containing activated carbon according to claim 1 and the nutritional agents of microorganism cenobium, its feature
It is：The complex enzyme that the compound biological enzyme compounds for two or more following any biology enzymes, including：Metalloprotease,
Alkaline phosphatase：Carbohydrase, amylase, acid protease, pectase, glucose oxidase, catalase, ribonucleic acid
Enzyme, lipase, cellulase, hesperidinase, naringinase.
4. the preparation method of a kind of enzyme containing activated carbon according to claim 1 and the nutritional agents of microorganism cenobium, its feature
It is：Length≤1mm after the agricultural organic waste crushing.
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| CN109206232A (en) * | 2018-10-25 | 2019-01-15 | 北京中农润田化肥有限公司 | Spore enzyme and spore enzyme series fertilizer and its application in agricultural |
| CN110577911A (en) * | 2019-09-17 | 2019-12-17 | 山东农业大学 | bacillus pumilus and application thereof |
| CN110776377A (en) * | 2018-07-30 | 2020-02-11 | 山东禹城瑞利源科技有限公司 | Polyporus agent, polyporus urea and series fertilizers thereof |
| CN111960886A (en) * | 2020-08-17 | 2020-11-20 | 黑龙江银米滩生物科技有限公司 | Compound microbial fertilizer and application thereof |
| CN112175933A (en) * | 2020-09-29 | 2021-01-05 | 华南农业大学 | Biological carbon-rose-smoky cordyceps nanoparticle and preparation method and application thereof |
| CN112573970A (en) * | 2020-11-05 | 2021-03-30 | 东北农业大学 | Solid-state compound microbial foliar fertilizer for improving saline-alkali soil and application thereof |
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| CN110577911A (en) * | 2019-09-17 | 2019-12-17 | 山东农业大学 | bacillus pumilus and application thereof |
| CN111960886A (en) * | 2020-08-17 | 2020-11-20 | 黑龙江银米滩生物科技有限公司 | Compound microbial fertilizer and application thereof |
| CN112175933A (en) * | 2020-09-29 | 2021-01-05 | 华南农业大学 | Biological carbon-rose-smoky cordyceps nanoparticle and preparation method and application thereof |
| CN112573970A (en) * | 2020-11-05 | 2021-03-30 | 东北农业大学 | Solid-state compound microbial foliar fertilizer for improving saline-alkali soil and application thereof |
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