CN111960886A

CN111960886A - Compound microbial fertilizer and application thereof

Info

Publication number: CN111960886A
Application number: CN202010827731.9A
Authority: CN
Inventors: 董晓堂
Original assignee: Heilongjiang Yinmitan Biotechnology Co ltd
Current assignee: Heilongjiang Yinmitan Biotechnology Co ltd
Priority date: 2020-08-17
Filing date: 2020-08-17
Publication date: 2020-11-20

Abstract

The invention discloses a compound microbial fertilizer, wherein each ton of the compound microbial fertilizer comprises the following components: 80-120kg of penicillium bailii, 40-60kg of trichoderma harzianum, 60-80kg of yeast, 800kg of glucose powder and 700kg of composite trace elements, and the microbial fertilizer obviously improves the stress resistance of plants.

Description

Compound microbial fertilizer and application thereof

Technical Field

The invention relates to the technical field of biological fertilizers, in particular to a compound microbial fertilizer and application thereof.

Background

The microbial fertilizer is developed according to the soil microecology principle, the plant nutrition principle and the basic concept of modern organic agriculture, contains various high-activity beneficial microbial bacteria, is suitable for various crops, can activate nutrients, improves the nutrient utilization rate, has broad universality and breaks the inherent weaknesses of the specificity, the limitation and the special fertilizer of the common biological fertilizer. The microbial fertilizer is a novel fertilizer biological product which enables crops to obtain required nutrients (fertilizer) through the life activities of active (reproducible) microorganisms, and is one of the fertilizers in agricultural production (also called third generation fertilizers).

However, the current research on the microbial fertilizer is not thorough, the quality of most of the microbial fertilizers is unstable, the target efficiency of the strains is unstable, and the stress resistance and disease resistance of plants are not ideal.

Therefore, the technical problem to be solved by the technical personnel in the field is how to provide a microbial fertilizer which can effectively improve the stress resistance of plants.

Disclosure of Invention

In view of the above, the invention provides a compound microbial fertilizer, which is prepared by compounding penicillium bailii, trichoderma harzianum and saccharomycetes as microbial strains, and the stress resistance of plants is remarkably improved.

In order to achieve the purpose, the invention adopts the following technical scheme:

the compound microbial fertilizer is characterized in that each ton of the compound microbial fertilizer comprises the following components: 80-120kg of penicillium bailii, 40-60kg of trichoderma harzianum, 60-80kg of yeast, 800kg of glucose powder and 700-40 kg of composite trace elements.

As a preferred technical scheme of the invention, each ton of the compound microbial fertilizer comprises the following components: 100kg of penicillium bailii, 50kg of trichoderma harzianum, 70kg of microzyme, 750kg of glucose powder and 30kg of composite trace elements.

The technical effects achieved by the technical scheme are as follows:

white hyphae grow out in the early growth stage of the penicillium bylysine, and cyan hyphae appear, which is a branched strain with the strongest disease resistance in penicillium, has obvious inhibition effect on fusarium oxysporum and verticillium dahliae, has obvious prevention effect on fungal diseases such as soil-borne diseases, root rot, root retting, downy mildew, leaf mold, gray mold and the like, has very good effect on promoting phosphorus activation, and has certain inhibition effect on root-knot nematodes;

the trichoderma harzianum can prevent and treat leaf fungal diseases such as powdery mildew, gray mold, downy mildew, leaf mold, leaf spot and the like of crops such as vegetables, fruit trees, flowers and the like in fields and greenhouses, and has the following fundamental action mechanism: the energy transfer required by the propagation and growth of pathogenic bacteria is blocked, the permeability is increased to dry the fungal spores, and meanwhile, the cell membranes are damaged to destroy the growth of spore germination tubes;

the yeast contains special oxidative decomposition enzyme in vivo, so the yeast has strong metabolic capability, and harmful substances in soil are decomposed by hydrolase on the surface of cells, so the yeast has the effect of soil remediation;

the glucose powder provides energy and nutrient substances for the growth and metabolism of the penicillium bailii, trichoderma harzianum and saccharomycetes, so that microorganisms can fully play a role in soil;

the composite trace elements contain various trace elements, so that the stress resistance of plants can be improved, and the photosynthesis and lodging resistance of the plants can be enhanced.

As a preferred technical scheme of the invention, the composite trace elements are purchased from Xintengke biological feed Co.

The compound microbial fertilizer is applied to the preparation of fertilizers for improving the stress resistance of plants.

The compound microbial fertilizer is applied to the preparation of fertilizers for improving the crop yield.

The compound microbial fertilizer is applied to preparing a fertilizer for improving soil quality.

In conclusion, the compound microbial fertilizer has the following effects:

1) the composite fungi produce a large amount of organic phosphorus decomposing enzyme, so that the organic phosphorus in the soil can be fully absorbed and utilized by plants. The solubility of calcium phosphate and the like in water can be improved by 500 times, and the in-season utilization rate of phosphate fertilizers such as diammonium hydrogen phosphate and the like is improved by more than 3 times; the application amount of phosphate fertilizer can be reduced by 40-60% on different crops; the phosphorus content in the organic-inorganic compound fertilizer or the compound fertilizer can be 6-8%; 4-6% of phosphorus content can be achieved on the bio-organic fertilizer;

2) the composite fungus in the invention generates enzyme, capsular polysaccharide and biological acid substances, can decompose and convert mineral potassium and fixed potassium in soil into effective potassium which can be absorbed by plants, and can be complexed with silicon dioxide in soil to promote degradation of minerals, thereby releasing silicon and potassium surrounded by mineral crystal lattices. The utilization rate of potassium fertilizers such as potassium chloride, potassium sulfate and the like in season is improved by 30-50%; the application amount of potash fertilizer can be reduced by 30-40% on different crops; the potassium content in the organic-inorganic compound fertilizer or the compound fertilizer can be 3-5 percent; can be used as 3-4% of potassium content in the bio-organic fertilizer.

3) The metabolic activity of the composite fungi can generate phytohormones such as gibberellin, indoleacetic acid, cytokinin, phenolic compounds and derivatives thereof, and the like, and can also generate acidic substances such as nicotinic acid, pantothenic acid and the like and active substances such as vitamins and the like, and the substances can play a role in stimulating the growth of crops. The fungi and the plant root system are symbiotic to form endogenetic and ectogenic mycorrhiza, so that the growth of the plant root system is promoted, and the root system is stout and flourishing; the plant is tall and big, the leaves are fat and thick, and the color and luster are dark green; the growth period is shortened; full seeds and full fruits. Can obviously strengthen the synthesis and operation of the plants on sugar, starch, protein and various vitamins, and increases the synthesis and accumulation of starch, polysaccharide and monosaccharide. The content of sugar and VC in vegetables and fruits are improved, the content of nitrate in the vegetables and fruits is reduced, the dry matter content of the vegetables and fruits is increased, the increase of amylase and sucrose acid invertase in plants is promoted, and the soluble solid of the fruits is improved.

4) The composite fungus can induce crops to generate substances such as superoxide dismutase, peroxidase and the like with the effect of improving the stress resistance of the crops, and when the crops are stressed by adverse conditions such as drought, salt and alkali, insect pests, diseases, aging and the like, the damage of environmental stress to the crops is relieved by eliminating free radicals of the crops. The drought resistance of dry field crops such as corn, wheat and soybean is improved; the saline-alkali resistance of crops planted in saline-alkali soil is improved; the disease resistance of rice seedlings such as rice seedbeds is enhanced. Strengthen the plants of fruits and vegetables, improve the transportation and transformation rate of water and mineral nutrition by multiple times, promote the activity of root systems, improve the oxidizing capacity of the root systems, keep the micro-ecological environment balance of the root systems and prevent the occurrence of root diseases.

5) The beneficial microorganisms in the composite fungi degrade harmful substances, complex heavy metals and decompose residual harmful substances of organic pesticides and herbicides through life activities. The harm of continuous cropping to the growth of crops is reduced by degrading harmful pollutants in soil, and the soil is purified. The saline-alkali soil and hardened soil can be gradually fluffed, the physical and chemical properties of the soil can be thoroughly improved, the organic components of the soil are increased, ventilation is facilitated, and the purpose of water storage and fertilizer conservation of the soil is achieved.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Trichoderma harzianum used in the present invention was purchased from Shandong Changtai Biotech Co., Ltd; penicillium bailii was purchased from Shanghai Egyiki Biotech limited; the yeast is purchased from subnata; the composite trace elements were purchased from biological feeds, Inc. of Xintengke, Guangzhou.

Example 1

The compound microbial fertilizer is characterized in that each ton of the compound microbial fertilizer comprises the following components: 120kg of penicillium bailii, 60kg of trichoderma harzianum, 80kg of microzyme, 700kg of glucose powder and 40kg of composite trace elements.

Example 2

The compound microbial fertilizer is characterized in that each ton of the compound microbial fertilizer comprises the following components: 80kg of penicillium bailii, 40kg of trichoderma harzianum, 60kg of microzyme, 800kg of glucose powder and 20kg of composite trace elements.

Example 3

The compound microbial fertilizer is characterized in that each ton of the compound microbial fertilizer comprises the following components: 100kg of penicillium bailii, 50kg of trichoderma harzianum, 70kg of microzyme, 750kg of glucose powder and 30kg of composite trace elements.

The preparation method of the compound microbial fertilizer in the embodiments 1-3 comprises the following steps:

weighing the raw materials according to the weight parts, mixing, and fully and uniformly stirring to obtain the compound microbial fertilizer.

Example 4

The compound bacterial manure prepared in the examples 1 to 3 is dissolved in water to prepare a compound bacterial manure solution with the concentration of 50 mug/ml, and the following experiments are carried out:

test groups: spraying the leaves in the fruit expanding period and the coloring period of the crown pears respectively, wherein the spraying standard is that the leaves are full of water drops but the water drops are not left;

control group: spraying clean water with the same volume;

when the fruits are ripe, 10 fruits are adopted in each treatment, the yield and the quality of the fruits are observed, and the average weight (g) and the specific gravity (g/ml) of each fruit are counted; and counting the disease index of the drought blight of the Huangguan pears in the mature period, which is shown in table 1;

TABLE 1

As can be seen from Table 1, the weight and specific gravity of a single fruit can be obviously improved by spraying the composite bacterial fertilizer disclosed by the invention, and the disease index of the fruit in the mature period of the drought blight can be reduced.

Example 5

Respectively dissolving the compound microbial fertilizer prepared in the examples 1-3 with water to prepare a compound microbial fertilizer solution with the concentration of 50 mug/ml, performing root irrigation treatment on tomato seedlings, and counting the weight of each fruit of the tomato, the incidence index of bacterial wilt of the seedlings and the total yield of the tomato per mu; and using clear water as a reference; the results are shown in Table 2;

TABLE 2

As can be seen from the table 2, the compound microbial fertilizer disclosed by the invention can obviously improve the yield of single fruits and the yield per mu of tomatoes and effectively reduce the morbidity index of bacterial wilt.

Example 6

Taking the compound fertilizer in example 3 as an example, the components in example 3 are mixed to obtain a compound microbial fertilizer, the compound microbial fertilizer is applied to the soil of the saline-alkali soil (the pH value of the soil and the organic matter content in the soil are measured before the compound microbial fertilizer is applied), the application amount is 250 kg/mu, and the pH value and the organic matter content of the soil are measured after 6 months, and the results are shown in table 3;

TABLE 3

As can be seen from Table 3, the compound microbial fertilizer of the invention can significantly improve the physicochemical properties of soil.

Example 7

7kg of yeast, 10kg of lysine penicillium, 75kg of glucose powder and 8kg of composite trace elements form a composite microbial fertilizer 1;

7kg of yeast, 5kg of trichoderma harzianum, 75kg of glucose powder and 13kg of composite trace elements form a composite microbial fertilizer 2;

preparing the compound microbial fertilizer 1 and the compound microbial fertilizer 2 into a compound microbial fertilizer solution with the concentration of 50 mu g/ml, performing root irrigation treatment on tomato seedlings, and counting the single fruit weight and the acre yield of the tomatoes and the incidence rate of tomato bacterial wilt; the results are shown in Table 4;

TABLE 4

As is clear from Table 4, the synergistic effect between the Penicillium lypocarum and Trichoderma harzianum of the present invention is shown by referring to example 5.

The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. The compound microbial fertilizer is characterized in that each ton of the compound microbial fertilizer comprises the following components: 80-120kg of penicillium bailii, 40-60kg of trichoderma harzianum, 60-80kg of yeast, 800kg of glucose powder and 700-40 kg of composite trace elements.

2. The compound microbial fertilizer according to claim 1, wherein each ton of compound microbial fertilizer comprises the following components: 100kg of penicillium bailii, 50kg of trichoderma harzianum, 70kg of microzyme, 750kg of glucose powder and 30kg of composite trace elements.

3. The compound microbial fertilizer according to any one of claims 1-2, wherein said compound trace elements are obtained from biological feeds of the department of protradae, guangzhou.

4. The application of the compound microbial fertilizer as claimed in claim 3 in preparing a fertilizer for improving stress resistance of plants.

5. Use of the compound microbial fertilizer as defined in claim 3 in the preparation of a fertilizer for increasing crop yield.

6. Use of the compound microbial fertilizer as defined in claim 3 in the preparation of a fertilizer for improving soil quality.