CN104152149A - Novel multifunctional composite biological soil improvement agent - Google Patents
Novel multifunctional composite biological soil improvement agent Download PDFInfo
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- CN104152149A CN104152149A CN201410385438.6A CN201410385438A CN104152149A CN 104152149 A CN104152149 A CN 104152149A CN 201410385438 A CN201410385438 A CN 201410385438A CN 104152149 A CN104152149 A CN 104152149A
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Abstract
The invention relates to a green and efficient biological organic soil improvement agent, in particular to a novel composite biological soil improvement agent. The novel multifunctional composite biological soil improvement agent is characterized by being prepared by uniformly mixing the following raw materials in parts by weight: 20-40 parts of rhizopus stolonifer fermentation liquid, 20-40 parts of trichoderma viride fermentation liquid, 1.5-2.5 parts of water-soluble chitin and 1.5-4.5 parts of potassium fulvic. The soil improvement agent mainly has the functions that the photosynthesis capacity of plants can be enhanced, and the directional transportation of photosynthetic products is facilitated; the root growth is promoted, and the yield is increased by 20% to 30%; the fruits are attractive, the color is bright, and the content of sugar and vitamins is increased; the soil pH value is increased; acidified soil, hardened soil and salinity soil are improved; the soil nutrient is activated, and the soil fertility is released.
Description
Technical field
What the present invention relates to is a kind of biological organic soil conditioner of green high-efficient, particularly a kind of NEW TYPE OF COMPOSITE biological soil conditioner.
Background technology
China is a large agricultural country, and soil resource is support crop growth basic.Therefore, soil resource is the prerequisite that people depend on for existence, is also the basis of Economic development and human culture progress.Along with the develop rapidly of China's economy and the raising of population increase and living standards of the people, people increase the demand of grain, vegetables, fruit etc., and the status of soil resource is more and more outstanding.In order to meet people's demand, peasant's raising crop yield that makes in all sorts of ways, for example: use a large amount of agriculture chemicals, continuous cropping plantation, intensive cropping etc.At short notice, these agricultural measures can improve crop yield, in the long run, and for the utilization of soil, it has produced severe side effect, for example: Soil degradation, soil compaction, soil productivity reduce, crop quality declines, pesticide residue increase, poisoning fertilizer damage is serious etc.These agricultural measures seriously hinder the development of sustainable agriculture.
Under this background, for how keeping soil quality, improving soil acid-base equilibrium, remove soil toxicity, reducing the problems such as soil-borne disease propagation, raising crops quality, production organic products become the focus that people pay close attention to gradually.The correct soil improvement agent that uses can effectively be alleviated above problems, so the production of soil improvement agent, use and study the favor that is more and more subject to people, but, because China's soil type is various, lack eurytropy and narrow spectrum soil improvement agent product, soil improvement agent product category is a lot, and research and development of products material is single, and because Technology problem causes quality product, effect, unstable properties.The raw material of soil improvement agent focuses mostly in natural and synthetic material, few to the exploitation of domestic refuse, biomass waste, and in this case, a large amount of cheap soil improvement starting material are put aside as useless, and contaminated soil and water body.The biological modification agent of China is of a great variety at present, but the screening of high-efficiency strain and pure culture problem are still the bottlenecks of its development of restriction.
Current soil improvement agent on the market is mainly divided into mineral substance, natural and semi-synthetic water-soluble polymer class, synthetic macromolecular compound and effective microorganism preparation class etc.Mineral substance soil improvement agent cost is low, and still, it can have a strong impact on soil microorganisms structure, especially the structure of community of beneficial microorganism and Biocontrol microorganism.Synthetic macromolecular compound class soil improvement agent cost is high, if improper use, its meeting generating portion is residual, contaminated soil.Present effective microorganism preparation is on the market mostly the preparation of single culture, and cost is higher.
Summary of the invention
The present invention is intended to solve the problem such as the effect of Soil degradation and existing soil improvement agent is single, cost is higher, and then a kind of pollution-free, efficient, practical Multifunction compound biological soil improver is provided.
Multifunction compound biological soil improver of the present invention, centered by activity fungal, and add N,O-CMC, potassium fulvate, can regulate soil microorganisms flora structure, improve soil physico-chemical property, controlling plant diseases, promote plant root growth, for crop growth provides necessary nutritive substance, and can environment not produced and be polluted, be a kind of biological organic soil conditioner of green high-efficient.
Concrete technical scheme of the present invention is as follows:
A kind of Multifunction compound biological soil improver, its special character is, is evenly mixed and makes by the feed composition of following weight part meter:
When use, aforesaid combination dilution agent is become to suitable concentration, before field planting, be sprayed on soil surface, fill with root, this composition has spectrum effect, prevent and treat soil-borne disease, the poisoning of degraded fertilizer damage, the Plant diseases that control fungi, bacterium, virus cause, effectively improves the Soil degradation problems such as the hardening of soil, acidifying, salinification, and various crop is had to obvious production-increasing function.
Described rhizopus stolonifer fermented liquid is to be made by rhizopus stolonifer cultivation and fermentation;
Rhizopus stolonifer (Rhizopus stolonifer), preserving number is: CGMCC No.8435, preservation name is called: rhizopus stolonifer PF102 (Rhizopus stolonifer), preservation date is: on November 5th, 2013, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center.
Described Trichoderma Viride liquid is to be made by viride cultivation and fermentation;
Viride (Trichoderma viride), preserving number is: CGMCC No.8434, preservation name is called: viride PF1 (Trichoderma viride).Preservation date is: on November 5th, 2013, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center.
The cultivation and fermentation process of described rhizopus stolonifer fermented liquid and Trichoderma Viride liquid is as follows:
1. with transfering loop, cryopreserved rhizopus stolonifer bacterial classification or viride bacterial classification are inoculated in to dull and stereotyped PDA substratum (potato 200g, sucrose 20g, agar 18g, distilled water 1000mL), 3 generations of switching;
2. transfer in PDB substratum (potato 200g with transfering loop picking edge mycelia, sucrose 20g, distilled water 1000mL) in, 25 DEG C~30 DEG C of culture temperature, optimum temperuture is 28 DEG C, 120rpm, and the time is 10 days, fermented liquid 100 μ m sieves filter and obtain spore suspension, and the spore concentration of living is 5-9 × 10
8individual/ml.
3. the spore suspension 2. step being obtained is inoculated in the fermentor tank that fills PDB substratum or bran mass, carries out liquid submerged fermentation, obtains rhizopus stolonifer fermented liquid and Trichoderma Viride liquid.
Viride and rhizopus stolonifer can be secreted VITAMIN, amino acid, saccharifying enzyme, proteolytic enzyme, specific enzymes system etc. and have the active substance of certain physiological function during the fermentation, improve soil microorganisms flora structure, promote the growth of root system of plant, strengthen the receptivity of root system to nutritive ingredient, these two kinds of hypha,hyphaes can firmly adhere to and be wound around with soil particle, improve soil stability and porosity; Potassium fulvate has high capacity amount and physiologically active, and it is plant utilization better that its chelating constant and trace nutrient make it, and slow release fertilizer, improves dietetic alimentation, promotes plant germination and growth, accelerates precipitation and decomposes, and improves Soil structure.
The present invention is component centered by rhizopus stolonifer and viride, and adds potassium fulvate and N,O-CMC.By using the microorganism with Biocontrol Effect, reach the object that regulates microbial bacteria group structure and prevent and treat soil-borne disease; By using potassium fulvate, N,O-CMC, reach the disease resistance that improves farm crop, hinder the multiplication rate of soil-borne pathogen, improve soil physico-chemical property, promote the decomposition of the soil organism and the release of nutritive ingredient, regulate soil pH, strengthen the receptivity of root system of plant to nutritive substance and promote the object of its growth.Two kinds of bacterial strain reproduction speeds that use in the present invention are very fast and have high exocytosis activity, utilize liquid submerged fermentation technology to realize large-scale production, can obtain fast a large amount of thalline and spore, reduced production cost, easy to use.
The resistance of rhizopus stolonifer (Rhizopus stolonifer) is extremely strong, in Fast-propagation process, can produce the active substance of the multiple decomposition soil organism and soil effective constituent, for example: VITAMIN, amino acid, saccharifying enzyme, proteolytic enzyme, amylase etc.Rhizopus stolonifer for example can produce the outer active substance of a large amount of born of the same parents: the multiple compounds such as amino acids, nucleic acid, VITAMIN, sphingolipid, and they have effects such as decomposing organic, inhibition harmful microorganism, promotion plant root growth; The all right multiple antibacterial substance of external secretion of rhizopus stolonifer, it has and suppresses active multiple pathogenic bacteria, has spectrum, and strong stress resistance, and both preventable diseases, can promote again plant strain growth.
(Trichoderma viride) is widely distributed for viride, and its secretory product has Promoting plant growth and sterilization effect, has the great bacterial strain that improves Soil capabilities.Viride has abundant mycelia, its mycelia can be wound around soil particle, and then stability and the permeability of raising soil, break soil compaction and sclerosis, viride also can be secreted the outer macromolecule polysaccharide of abundant born of the same parents and glycoprotein and highly active specific enzymes system, regulate soil acid-base equilibrium, promote soil to discharge nutrient, promote the absorption of root system of plant to nutritive ingredient.
The adaptability of rhizopus stolonifer and viride is very strong, between 4 DEG C~42 DEG C, all can grow, and reproduction speed is surprising, and this is conducive to them and captures fast space resources and nutritional resource.In addition, these two kinds of fungal bacterial strains are easy to cultivate under artificial condition, and the kind that can be used for pathogenic bacteria control is a lot.
A kind of Multifunction compound biological soil improver of the present invention, cost is lower, mainly contains following functions: can strengthen plant photosynthesis ability, promote the directed transport of photosynthate.Promote root growth, improve output 20-30%.Fruit is attractive in appearance, and color and luster is beautiful, and sugar and vitamin contents increase; Improve soil pH value; Improvement acidified soil, soil and salinization soil harden; Activating soil nutrient, discharges soil fertility.Impel soil aggregate to increase 5%--30%, soil porosity and Gas permeability increase by 10%, optimize root growth environment, impel well developed root system; Can accelerate the breeding of soil beneficial microorganism, suppress disease microbial activities, prevent and treat soil-borne disease, strengthen crop anti-adversity, system is used can well prevent and treat the Plant diseases causing because of fungi, bacteriophage.Poisoning, the fertilizer damage that can effectively degrade in soil, have obvious prevention effect to the blossom-end rot of tomato, capsicum etc.; Reduce the harm such as samping off, blight, root knot nematode.
Embodiment
Below provide the specific embodiment of the present invention, be used for formation of the present invention to be further described.
Biological liquid preparation specific embodiment of the present invention is as follows:
Example of formulations 1 product
20 parts of rhizopus stolonifer fermented liquids, 20 parts of Trichoderma Viride liquid, 1.5 parts of N,O-CMCs, 1.5 parts of potassium fulvates.
Example of formulations 2 products
30 parts of rhizopus stolonifer fermented liquids, 30 parts of Trichoderma Viride liquid, 2 parts of N,O-CMCs, 3 parts of potassium fulvates.
Example of formulations 3 products
40 parts of rhizopus stolonifer fermented liquids, 40 parts of Trichoderma Viride liquid, 2.5 parts of N,O-CMCs, 4.5 parts of potassium fulvates.
Test example 1
The impact (Zhaoyuan, shandong) of soil improvement agent on soil pH, the soil weight and porosity
Test group is treated to 1000ml/ mu (treatment group 1), 1500ml/ mu (treatment group 2) soil improvement agent, front evenly the spraying in soil surface of turning over, and control group sprays equivalent clear water, and the follow-up processing of topdressing is identical, repeats 3 times.As shown in Table 1, use soil improvement agent after the soil weight significantly decline, soil porosity significantly increases, pH value approaches neutral, soil acidification situation is reversed, and is suitable for plant growth.
The impact of table one soil improvement agent on soil physico-chemical property
Test example 2
The impact (Shandong Shouguang) of soil improvement agent on soil enzyme activities
Soil surface sprays the soil improvement agent of 1000ml/ mu, 1500ml/ mu, front evenly the spraying in soil surface of turning over, and control group sprays equivalent clear water.Soil enzyme activities height is one of important indicator of soil fertility, the Main Function of soil enzyme: participate in material cycle and transmission ofenergy in soil, affect soil fertility, improve edatope together with soil microorganisms.Soil enzyme participates in organic decomposition and conversion, promotes the circulation of the important elements such as carbon, nitrogen phosphate and sulfur, soil characteristic is played an important role simultaneously.Can be found out by table two, the vigor of catalase, acid phosphatase, urase and four kinds of enzymes of saccharase in soil of using after soil improvement agent all has lifting, shows that biological activity of soil improves.
Table two: the impact of soil improvement agent on soil enzyme activities
Test example 3
The impact (Zhaoyuan, shandong) of soil improvement agent on Soil Microorganism quantity
Soil surface sprays the soil improvement agent of 1000ml/ mu, 1500ml/ mu, front evenly the spraying in soil surface of turning over, and control group sprays equivalent clear water.Soil microorganisms is the important indicator of soil quality.Soil Microorganism quantity is many, breeding is fast, reactivity is strong, and plant is had to very important impact.Soil microorganisms not only can regulate plant strain growth, and has vital role at the aspect such as maintenance, raising and Conversion of energy and material cycle of soil fertility.As shown in Table 3, this product has improved Soil Microorganism quantity, has increased microbial diversity.
The impact of table three soil improvement agent on peanut Rhizospheric microorganisms' quantity
Test example 4
The impact (Distributions in Liaocheng of Shandong Province) of soil improvement agent on cucumber soil nutrient
Soil surface sprays the soil improvement agent of 1000ml/ mu, front evenly the spraying in soil surface of turning over, and control group sprays equivalent clear water.Soil nutrient is the important symbol of soil fertility, is the basis of plant growth.As shown in Table 4, this product is improved to nutrient content in soil, has promoted the release of soil nutrient.
The impact of table four soil improvement agent on cucumber yield and soil nutrient content
Test example 5
The impact (Distributions in Liaocheng of Shandong Province) of soil improvement agent on Tomato Quality
Soil surface sprays the soil improvement agent of 1000ml/ mu, front evenly the spraying in soil surface of turning over, and control group sprays equivalent clear water.Use after soil improvement agent, can make to a certain extent nitrate content contained in tomato reduce, the generation that significantly improve soluble proteins in tomato, soluble sugar, Vitamin C content, can effectively alleviate blight, is that Speed of disease slows down.
The impact of table five soil improvement agent on Tomato Quality
Test example 6
The prevention effect (Shandong Yantai) of soil improvement agent to apple tree canker
Process autumn, and every 1000ml soil improvement agent for apple tree (diluting 100 times) carries out root irrigation, and control group sprays equivalent clear water.The multiple sickness rate of scab is low, and prevention effect is good.
The prevention effect of table 6 soil improvement agent to apple tree canker
Test example 7
The impact (Jining of Shandong Province) of soil improvement agent on Yield Traits In Corn and soil phosphorus
Soil surface sprays the soil improvement agent of 1000ml/ mu, front evenly the spraying in soil surface of turning over, and at Maize at Seedling Stage, with 1000ml/ mu soil improvement agent (the diluting 100 times) processing of spraying, control group sprays equivalent clear water.After results, the measurement result of soil phosphorus is shown, this soil improvement agent can activating soil insoluble phosphorus, improves phosphate fertilizer utilization efficiency.
The impact of table 7 soil improvement agent on Yield Traits In Corn and soil phosphorus
Claims (4)
1. a Multifunction compound biological soil improver, is characterized in that, includes rhizopus stolonifer fermented liquid and Trichoderma Viride liquid.
2. a Multifunction compound biological soil improver, is characterized in that, is evenly mixed and makes by the feed composition of following weight part meter:
。
3. a kind of Multifunction compound biological soil improver as claimed in claim 2, is characterized in that, the cultivation and fermentation process of described rhizopus stolonifer fermented liquid and Trichoderma Viride liquid is as follows:
1. with transfering loop, cryopreserved rhizopus stolonifer bacterial classification or viride bacterial classification are inoculated in to dull and stereotyped PDA substratum (potato 200g, sucrose 20g, agar 18g, distilled water 1000mL), 3 generations of switching;
2. transfer in PDB substratum (potato 200g with transfering loop picking edge mycelia, sucrose 20g, distilled water 1000mL) in, 25 DEG C~30 DEG C of culture temperature, optimum temperuture is 28 DEG C, 120rpm, and the time is 10 days, fermented liquid 100 μ m sieves filter and obtain spore suspension, and the spore concentration of living is 5-9 × 10
8individual/ml.
3. the spore suspension 2. step being obtained is inoculated in the fermentor tank that fills PDB substratum or bran mass, carries out liquid submerged fermentation, obtains rhizopus stolonifer fermented liquid and Trichoderma Viride liquid.
4. a kind of Multifunction compound biological soil improver as claimed in claim 3, it is characterized in that, described rhizopus stolonifer (Rhizopus stolonifer), preserving number is: CGMCC No.8435, preservation name is called: rhizopus stolonifer PF102 (Rhizopus stolonifer), preservation date is: on November 5th, 2013, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center;
Described viride (Trichoderma viride), preserving number is: CGMCC No.8434, preservation name is called: viride PF1 (Trichoderma viride), preservation date is: on November 5th, 2013, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center.
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Cited By (6)
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CN106867542A (en) * | 2015-12-14 | 2017-06-20 | 灵武市森保科技开发有限公司 | A kind of facilities vegetable continuous cropping soil conditioner and preparation method thereof |
CN106957870A (en) * | 2017-03-17 | 2017-07-18 | 山东普方生物科技有限公司 | A kind of composite biological agent and application thereof |
MD4486C1 (en) * | 2016-01-21 | 2017-12-31 | Национальный Центр Общественного Здоровья Министерства Здравоохранения Республики Молдова | Strain of Rhizopus stolonifer fungi for biodegradation of cobalt and nickel compounds |
CN108587962A (en) * | 2018-05-01 | 2018-09-28 | 西安德龙生物科技有限公司 | Purposes of Bacillus subtillis B2-3-2 and the B19 composite bacteria agent in resisting banana vascular wilt |
CN111205136A (en) * | 2020-03-09 | 2020-05-29 | 杜得信 | Soil conditioner containing sodium glutamate and amino-oligosaccharin |
CN116348434A (en) * | 2020-08-04 | 2023-06-27 | 美国赛多美科技有限公司 | Plant fertilizer compositions for improving phosphorus solubility and related methods |
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Cited By (10)
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CN106867542A (en) * | 2015-12-14 | 2017-06-20 | 灵武市森保科技开发有限公司 | A kind of facilities vegetable continuous cropping soil conditioner and preparation method thereof |
CN106867542B (en) * | 2015-12-14 | 2020-04-21 | 灵武市森保科技开发有限公司 | Soil conditioner for facility vegetable continuous cropping and preparation method thereof |
MD4486C1 (en) * | 2016-01-21 | 2017-12-31 | Национальный Центр Общественного Здоровья Министерства Здравоохранения Республики Молдова | Strain of Rhizopus stolonifer fungi for biodegradation of cobalt and nickel compounds |
CN106957870A (en) * | 2017-03-17 | 2017-07-18 | 山东普方生物科技有限公司 | A kind of composite biological agent and application thereof |
CN109456900A (en) * | 2017-03-17 | 2019-03-12 | 山东普方生物科技有限公司 | A kind of composite biological agent and application thereof |
CN109456900B (en) * | 2017-03-17 | 2022-09-20 | 山东普方生物科技有限公司 | Composite biological preparation and application thereof |
CN108587962A (en) * | 2018-05-01 | 2018-09-28 | 西安德龙生物科技有限公司 | Purposes of Bacillus subtillis B2-3-2 and the B19 composite bacteria agent in resisting banana vascular wilt |
CN108587962B (en) * | 2018-05-01 | 2021-09-28 | 西安德龙生物科技有限公司 | Application of bacillus subtilis B2-3-2 and B19 composite microbial inoculum in resisting banana wilt |
CN111205136A (en) * | 2020-03-09 | 2020-05-29 | 杜得信 | Soil conditioner containing sodium glutamate and amino-oligosaccharin |
CN116348434A (en) * | 2020-08-04 | 2023-06-27 | 美国赛多美科技有限公司 | Plant fertilizer compositions for improving phosphorus solubility and related methods |
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Application publication date: 20141119 |