CN103695321A - Microbe, application of microbe in bio-fertilizer preparation, bio-fertilizer and application of bio-fertilizer - Google Patents
Microbe, application of microbe in bio-fertilizer preparation, bio-fertilizer and application of bio-fertilizer Download PDFInfo
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Abstract
The embodiment of the invention provides a microbe, an application of the microbe in bio-fertilizer preparation, a bio-fertilizer and an application of the bio-fertilizer. Compared with the existing microbe for the bio-fertilizer and the existing bio-fertilizer in the prior art, the microbe provided by the invention is rhizopus stolonifer PF102, so that the microbe can be used for preparation of the bio-fertilizer; when the bio-fertilizer used for crop cultivation, the bio-fertilizer can improve the output of the crops, greatly reduce the morbidity of the crops and death rate due to disease, and in the meantime improve the growth conditions of the crops such as microbial population at the crop rhizosphere, soil bulk density, soil porosity, length and width of the plant leaf, and fruit quantity.
Description
Technical field
The present invention relates to biological fertilizer field, in particular to microorganism and the application in preparing bio-feritlizer, bio-feritlizer and application thereof.
Background technology
In recent years, along with the reinforcement of people's environmental consciousness and the raising that quality of life is required, bio-feritlizer, owing to can environment not being produced and be polluted, be a kind of fertilizer of green high-efficient, has obtained development fast and application widely in farm crop are cultivated.
In correlation technique, microorganism conventional in bio-feritlizer has vinelandii: as root nodule bacterium; Phosphorus-solubilizing bacteria: as bacillus megaterium, Bacillus licheniformis, cured auspicious genus bacillus, subtilis; Potassium solubilizing bacteria: as colloid bacillus cereus.
But the bio-feritlizer that contains mentioned microorganism can not be when improving crop yield, the pathogenic bacterium of effectively preventing crop.
Summary of the invention
The object of the present invention is to provide microorganism and the application in preparing bio-feritlizer, bio-feritlizer and application thereof, to solve the above problems.
First aspect provides a kind of microorganism in an embodiment of the present invention, preserving number is: CGMCC No.8435, preservation name is called: rhizopus stolonifer PF102 (Rhizopus stolonifer), preservation date is: on November 5th, 2013, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, postcode: 100101.
Second aspect provides the application of mentioned microorganism in bio-feritlizer preparation in an embodiment of the present invention.
The third aspect in an embodiment of the present invention provides a kind of bio-feritlizer, comprises mentioned microorganism or is derived from the mutant of described microorganism.
Further, described bio-feritlizer also comprises decomposed organic matter.
Particularly, described bio-feritlizer is made by the raw material of following weight part: the spore suspension of described microorganism: 5-15 part, decomposed organic matter: 30-50 part; Wherein, the spore concentration alive of described spore suspension is 3.0-5.0 * 10
10cfu/ml.
Particularly, described bio-feritlizer also comprises the chitin of 2-4 weight part.
More specifically, described bio-feritlizer also comprises the humic acid of 1-3 weight part.
Further more specifically, described bio-feritlizer also comprises the chitosan of 3-10 weight part.
Preferably, described bio-feritlizer is made by the raw material of following weight part: the spore suspension of described microorganism: 10 parts, and described decomposed organic matter: 45 parts, described chitin: 3 parts, described humic acid: 2 parts, described chitosan: 10 parts.
Fourth aspect in an embodiment of the present invention provides the application of above-mentioned bio-feritlizer in improving crop yield and controlling crop diseases and insect pests.
The microorganism that the above embodiment of the present invention provides and the application in preparing bio-feritlizer thereof, bio-feritlizer and application thereof, compare with the microorganism for bio-feritlizer of the prior art and bio-feritlizer, because microorganism is wherein rhizopus stolonifer PF102, therefore, preparation by this microorganism for bio-feritlizer, and by bio-feritlizer when the crop cultivation, not only can improve the output of crop, and the mortality ratio that can reduce greatly the sickness rate of crop and cause due to morbidity, can also improve rhizosphere microorganisms of crops quantity simultaneously, the soil weight, soil porosity, the length and width degree of plant leaf, the upgrowth situations such as fruit quantity.
Embodiment
Below by specific embodiment, the present invention is described in further detail.
Bio-feritlizer is that a kind of nutrient that integrates organic, inorganic nutritive element and microorganism is comprehensive, the balanced lasting compound manure of fertilizer efficiency.Because bio-feritlizer can not only provide crop growth required organic and inorganic nutrient, also can the microorganism by wherein to organism, degrade and nutrition transforms, produce the physiologically active substance that some can Promoting plant growth; In addition, because the raw material of bio-feritlizer is agricultural crop straw, municipal sludge, feces of livestock and poultry etc., development bio-feritlizer is conducive to alleviate the processing pressure of waste.
But in correlation technique, the microorganism in bio-feritlizer mostly can not be when improving crop yield, the pathogenic bacterium of effectively preventing farm crop.
The present invention is in order to address the above problem, screening has obtained a kind of microorganism (hereinafter to be referred as this microorganism), this microorganism in November 5 in 2013 China Committee for Culture Collection of Microorganisms common micro-organisms center carry out preservation, preserving number is: CGMCC No.8435, preservation name is called: rhizopus stolonifer PF102 (Rhizopus stolonifer).
This microorganism belongs to rhizopus stolonifer, in correlation technique, also do not find rhizopus stolonifer bacterial strain for bio-feritlizer, and for preventing and treating the pathogenic bacterium of crop.
This microorganism, for the preparation of bio-feritlizer, is applied to bio-feritlizer in the soil of plant growth, thereby improves the output of crop and the pathogenic bacterium of effectively preventing crop.
The bio-feritlizer that the embodiment of the present invention provides comprises this microorganism, and exists with the form of spore suspension.Wherein, spore suspension, spore concentration alive be 3.0-5.0 * 10
10cfu/ml.
The bio-feritlizer that the embodiment of the present invention provides also comprises decomposed organic matter.
Raw material in the bio-feritlizer that the embodiment of the present invention provides has following effect:
This microorganism belongs to head mold, has the feature of head mold.Head mold, in Fast-propagation process, can produce the several physiological active substances such as VITAMIN, amino acid, saccharifying enzyme, proteolytic enzyme, amylase, promotes the decomposition of soil with organic matter and the release of soil effective constituent.This microorganism can produce the outer active substance of abundant born of the same parents, comprises the multiple compounds such as amylase, proteolytic enzyme, amino acids, nucleic acid, VITAMIN, sphingolipid, and they have effects such as decomposing organic, inhibition harmful microorganism, promotion plant root growth; 2 kinds of antibacterial substances of this microorganism external secretion, lipopeptide antibiotic Surfactin and polyenoid class, have and suppress active multiple pathogenic bacteria, its meta-bolites strong stress resistance, both preventable disease, can promote plant strain growth again.Secondly, this microorganism adaptability is very strong, between 4 ℃～42 ℃, all can grow, and reproduction speed is surprising, and this is conducive to them and captures fast space resources and nutritional resource.In addition, this microorganism is easy to cultivate under artificial condition, and the kind that can be used for pathogenic bacteria control is a lot.
Chitin has the effect of " plant vaccine ", and continuously use can induce farm crop self to produce the resistance factor to disease, strengthens the anti-adversity abilities such as disease-resistant, anti-fall, anti-low temperature of plant.Chitin also can be used as slow-release fertilizer agent, improves chemical fertilizer utilization ratio, reduces disease incidence, significantly reduces agricultural chemicals usage quantity.In addition it also has extremely strong blade face adhesion property, can urge root strong sprout, promotes fruit maturation.
Chitosan can produce chitinase by inducing plant tissue, thereby hinders the propagation of phytopathogen.Wheat after treatment with chitosan and radish can obviously reduce the infringement of disease worm, and output increases significantly.With chitosan, can also synthesize plant prevention agent, it has good insecticidal mechanism, can suppress the breeding of insect.
Humic acid can dwindle the opening degree of pore, reduces moisture transpiration, makes the more moisture of plant and Soil conservation, promotes root system development simultaneously, improves improving activity of root system, makes the more moisture of Crop and nutriment.Improve plurality of enzymes activity and the chlorophyll content of plant materials, make metabolism vigorous, photosynthesis is strengthened, and sugar and dry-matter increase, thereby reach, improves crop yield, improves quality, improves the effect of the anti-adversity ability such as cold-resistant, disease-resistant.
In the embodiment of the present invention, by preferably preparing the ratio of raw material and the parts by weight of bio-feritlizer, while making the bio-feritlizer that obtains for plant growth, not only can increase substantially the output of crop, and the mortality ratio that can reduce greatly the sickness rate of crop and cause due to morbidity, can also improve the upgrowth situations such as length and width degree, fruit quantity of rhizosphere microorganisms of crops quantity, the soil weight, soil porosity, plant leaf simultaneously.
Embodiment 1, the screening of this microorganism
1, material
For trying soil: from the same continuous cropping cucumber planting greenhouse in Shouguang City, Shandong Province, push the rhizosphere soil that table soil gathers 3-15cm aside, the about 100g of every sample.Pedotheque is numbered respectively, packed, take back 4 ℃, laboratory Refrigerator store.
For trying substratum: PDA substratum, rose bengal medium.
2, the preparation of Soil Slurry
Pedotheque is put into sterilized mortar, in aseptic operating platform, be ground to uniform particles.Adopt 10 coubling dilutions, first take the ground soil of 1g, put into the abundant vibration of the triangular flask 30min that 99mL sterilized water is housed, make the soil diluent of 10-2, take out again 1mL and be added in 9mL sterilized water the diluent that 10-3 is made in fully vibration, by that analogy, make the diluent of 10-4 standby.
3, plating
Adopt spread plate, get respectively 10
-2, 10
-3, 10
-4diluent 0.1mL add in PDA substratum and rose bengal medium, with glass spreading rod, diluent is uniformly coated with and is opened, in 28 ℃ of constant incubators, cultivate, each soil sample repeats 3 times, each extent of dilution repeats 2 times.
4, the purifying of this microorganism and preservation
Observe colony growth situation and record original cultivation results.Growth characteristics according to microorganism on substratum and colonial morphology, picking list bacterium colony, separation and purification microorganism.Through repeatedly repeating aforesaid operations, obtain this microorganism of purifying, be then stored in-80 ℃ of refrigerators.
5, the evaluation of this microorganism
The method of identifying in conjunction with the fungi of this area routine: 1. morphological observation method; 2. genetic analysis method; The chromatographic process of the lipid acid feature of 3. analyzing and researching, determines that this microorganism is rhizopus stolonifer PF102.
6, the cultivation of going down to posterity of this microorganism
This microorganism is gone down to posterity in PDA substratum cultivation, 28 ℃, to cultivate and within 5 days, pass a generation, each culture thing all carries out amylase and proteinase activity is measured, and contrasts with the enzyme activity of original strain, usings this as index of investigating bacterial strain genetic stability.Wherein, adopt a-amylase activity measuring method to measure the amylase activity of this microorganism, adopt forint-phenol law to measure the proteinase activity of this microorganism.Result is as shown in the table.
As can be seen from the table, this microorganism strains filtering out was transferred for 10 generations continuously, obtain the bacterial strain of genetic stability.
Embodiment 2, the restraining effect of this microorganism to crop germ
For trying pathogenic bacterium: Fusarium oxysporum cucumber transformant, grey mold, verticillium dahliae, cucumber fusarium axysporum, dry thread Pyrenomycetes are (wherein, Fusarium oxysporum cucumber transformant can cause cucumber fusarium axysporum, grey mold can cause the gray mold of blade, verticillium dahliae can cause crop verticillium, and dry thread Pyrenomycetes can cause banded sclerotial blight).
For trying substratum: PDA substratum, PDB substratum, rose bengal medium.
In the embodiment of the present invention, adopt microorganism growth rate method to measure the bacteriostatic activity of the aseptic ferment filtrate of this microorganism.
This microbial inoculant of above-mentioned preservation, in PDB substratum, in 25 ℃, with the filtering with microporous membrane of 0.45 μ m is obtained to aseptic ferment filtrate after cultivating 8d under the condition of 160r/min.By the volume ratio of 1 ﹕ 4, filtrate and the PDA substratum that is cooled to the sterilizing of 50-60 ℃ are mixed, prepare aseptic ferment filtrate dull and stereotyped; Then pathogenic bacterium bacterium cake (general diameter is 7mm) is placed in to dull and stereotyped central authorities, take PDA culture medium flat plate as blank, under 25 ℃ of conditions, secretly cultivate, cultivate after 6-7 days, by right-angled intersection method, measure colony diameter, be calculated as follows relative bacteriostasis rate.With D1, represent the colony diameter obtaining on PDA culture medium flat plate, with D2, represent the colony diameter that obtains on aseptic ferment filtrate flat board to represent pathogenic bacterium bacterium cake diameter with D3.
This microorganism is to as shown in table 1 for the relative bacteriostasis rate of examination pathogenic bacterium:
This microorganism of table 1 is to the relative bacteriostasis rate for examination pathogenic bacterium
Embodiment 3, the preparation of this microbial spore suspension
Specifically be prepared as follows:
Cryopreserved microbial strains above transferred for 3 generations at PDA culture medium flat plate (potato 200g, sucrose 20g, agar 18g, distilled water 1000mL); Then use transfering loop picking the 3rd generation colony edge mycelia, transfer in PDB liquid nutrient medium (potato 200g, sucrose 20g, distilled water 1000mL), in, in 25 ℃～30 ℃, optimum temperuture is 28 ℃, 120rpm, condition under cultivate 10 days, obtain the fermented liquid of this microorganism.The fermented liquid obtaining is filtered and obtains spore suspension through 100 μ m sieves, and the spore concentration of living is 3.0
-5.0 * 10
10cfu/ml.
Embodiment 4, the preparation method of decomposed organic matter
In the embodiment of the present invention, decomposed organic matter can specifically be prepared as follows:
One or more butt raw material waters that form by 1000kg in the organic wastes such as crop material, leaf, weeds, sawdust, vinasse, furfural dregs water and irrigate, while making the water content of raw material reach 50-60%, by 5kg enzymatic microorganism powder, with the above-mentioned water content raw material blending that is 50-60%, pile the bar stack that wide 2 meters, high 1 meter, length are not limit, and build with plastic cloth.After 24 hours, make temperature in bar stack reach 60-70 ℃, after 48 hours, make temperature in bar stack reach 80-90 ℃, after 6-7 days, the temperature in bar stack is declined naturally, after 9-10 days, finish fermentation, obtain decomposed organic matter.
In order to improve the quality guaranteed period of decomposed organic matter, in the embodiment of the present invention, adopt aforesaid method, the decomposed organic matter obtaining after fermentation is air-dry in the cool, then with sack, installs standby.
As will be understood by the skilled person in the art, decomposed organic matter can also obtain according to the method for traditional organic waste retting,
Embodiment 5, comprise bio-feritlizer of this microorganism and preparation method thereof
The spore suspension that comprises this microorganism that embodiment 3 obtains, and this area conventional organic matter and inorganic fertilizer using while preparing bio-feritlizer.
As will be understood by the skilled person in the art, according to preparation method's preparation of the bio-feritlizer of this area routine, obtain.
Embodiment 6, comprise bio-feritlizer of this microorganism and preparation method thereof
The bio-feritlizer that comprises this microorganism is made by the raw material of following weight part: the spore suspension of this microorganism that embodiment 3 obtains: 5g, the decomposed organic matter that embodiment 4 obtains: 40g.
Above-mentioned raw materials is mixed according to proportioning, must contain the bio-feritlizer of this microorganism.
Embodiment 7, comprise bio-feritlizer of this microorganism and preparation method thereof
The spore suspension of this microorganism that embodiment 3 obtains: 15g, the decomposed organic matter that embodiment 4 obtains: 30g, chitin: 2g.
Above-mentioned raw materials is mixed according to proportioning, must contain the bio-feritlizer of this microorganism.
Embodiment 8, comprise bio-feritlizer of this microorganism and preparation method thereof
The spore suspension of this microorganism that embodiment 3 obtains: 10g, the decomposed organic matter that embodiment 4 obtains: 50g, chitin: 4g, humic acid: 3g.
Above-mentioned raw materials is mixed according to proportioning, must contain the bio-feritlizer of this microorganism.
Embodiment 9, comprise bio-feritlizer of this microorganism and preparation method thereof
The spore suspension of this microorganism that embodiment 3 obtains: 12g, the decomposed organic matter that embodiment 4 obtains: 40g, chitin: 3g, humic acid: 1g, chitosan: 3g.
Above-mentioned raw materials is mixed according to proportioning, must contain the bio-feritlizer of this microorganism.
Embodiment 10, comprise bio-feritlizer of this microorganism and preparation method thereof
The spore suspension of this microorganism that embodiment 3 obtains: 10g, the decomposed organic matter that embodiment 4 obtains: 45g, chitin: 3g, humic acid: 2g, chitosan: 10g.
Above-mentioned raw materials is mixed according to proportioning, must contain the bio-feritlizer of this microorganism.
It is example that embodiment of the present invention 11-17 be take the bio-feritlizer that above-described embodiment prepares, and narration bio-feritlizer is improving output and the application in control crop pathogenic bacterium of crop.
Embodiment 11, the impact of bio-feritlizer on red follicarpium sugarcane
Trial crops is divided into four groups: control group (CK, not bulk application); Fertilizer group (applying fertilizer as base manure, 100kg/ mu); Chemical fertilizer group (applying chemical fertilizer as base manure, 100kg/ mu); Bio-feritlizer group (applying bio-feritlizer that the embodiment of the present invention 5 provides as base manure, 100kg/ mu).Fertilization mode is all consistent with other farming methods, and in a conventional manner.
Every group of shared area of crop is 30m
2, every group arranges three repetitions, results averaged.Upgrowth situation and the output of red follicarpium sugarcane are as shown in table 2.
The impact of table 2 bio-feritlizer on the upgrowth situation of red follicarpium sugarcane and output
As can be seen from Table 2, upgrowth situation and the yield increased of the red follicarpium sugarcane of bio-feritlizer, fertilizer and chemical fertilizer that the use embodiment of the present invention 5 provides are significantly improved, and wherein, the bio-feritlizer that the embodiment of the present invention 9 provides is during as base manure, and it is maximum that red follicarpium sugarcane reaches best upgrowth situation and mu units increased in production.
Embodiment 12, the impact of bio-feritlizer on corn
Trial crops is divided into 2 groups: control group (CK, not bulk application); Bio-feritlizer group (applying bio-feritlizer that the embodiment of the present invention 8 provides as base manure, 100kg/ mu).Fertilization mode is all consistent with other farming methods, and in a conventional manner.
Every group of shared area of crop is 30m
2, every group arranges three repetitions, results averaged.Upgrowth situation and the output of corn are as shown in table 3.
The impact of table 3 bio-feritlizer on the upgrowth situation of corn and output
Upgrowth situation and the yield increased of the corn of the bio-feritlizer that as can be seen from Table 3, the use embodiment of the present invention 8 provides are significantly improved.
Embodiment 13, the impact of bio-feritlizer on cucumber Rhizospheric microorganisms' quantity
Trial crops is divided into two groups: control group (CK does not apply fertilizer); Bio-feritlizer group (applying the bio-feritlizer that the embodiment of the present invention 9 provides before field planting, 100kg/ mu).Fertilization mode is all consistent with other farming methods, and in a conventional manner.
Every group of shared area of crop is 30m
2, every group arranges three repetitions, results averaged.Kind and the quantity of cucumber rhizospheric microorganism are as shown in table 4.
The impact of table 4 bio-feritlizer on cucumber Rhizospheric microorganisms' quantity
As can be seen from Table 4, the bio-feritlizer that the embodiment of the present invention 9 provides apply the microbe population having improved in cucumber rhizosphere soil, increased microbial diversity.
Embodiment 14, the impact of bio-feritlizer on wheat yield and the soil weight and porosity
Trial crops is divided into 3 groups: control group (CK, not bulk application); Test group one (applying bio-feritlizer that the embodiment of the present invention 10 provides as base manure, 100kg/ mu); Test group two (applying bio-feritlizer that the embodiment of the present invention 10 provides as base manure, 150kg/ mu); Fertilization mode is all consistent with other farming methods, and in a conventional manner.
Every group of area 100m that crop is shared
2, every group arranges three repetitions, results averaged.After harvesting wheat, measure soil physico-chemical property and wheat yield, result is as shown in table 5,
The impact of table 5 bio-feritlizer on wheat yield and the soil weight and porosity
As can be seen from Table 5, compare with control group, after the bio-feritlizer that the use embodiment of the present invention 10 provides, the soil weight significantly declines, and soil porosity significantly increases, and pH value obviously increases and approaches neutral, soil acidification situation improves, and is suitable for wheat growth, and wheat yield obviously increases.
Embodiment 15, the impact of bio-feritlizer on the upgrowth situation of capsicum and output
Trial crops is divided into two groups: control group (CK does not apply fertilizer); Bio-feritlizer group (applying the bio-feritlizer that the embodiment of the present invention 10 provides before field planting, 100kg/ mu).Fertilization mode is all consistent with other farming methods, and in a conventional manner.
Every group of shared area of crop is 20m
2, every group arranges three repetitions, results averaged.Bio-feritlizer is as shown in table 6 on the impact of the upgrowth situation of capsicum and output.
The impact of table 6 bio-feritlizer on the upgrowth situation of capsicum and output
As can be seen from Table 6, compare with control group, the upgrowth situation of the capsicum of the bio-feritlizer that the use embodiment of the present invention 10 provides has clear improvement, and output is significantly increased.
Embodiment 16, the application of bio-feritlizer in control cucumber fusarium axysporum
Trial crops is divided into three groups: control group (CK does not apply fertilizer); Commercially available bio-feritlizer group (applying bio-feritlizer before field planting, 100kg/ mu); The bio-feritlizer group that the embodiment of the present invention 7 provides (applying bio-feritlizer before field planting, 100kg/ mu).Fertilization mode is all consistent with other farming methods, and in a conventional manner.
Every group of shared area of crop is 30m
2, every group arranges three repetitions, results averaged.The prevention effect of cucumber fusarium axysporum and the variation of output are as shown in table 7.
The prevention effect of table 7 cucumber fusarium axysporum and the variation of output
As can be seen from Table 7, use bio-feritlizer of the present invention to be all significantly improved than the commercially available bio-feritlizer of use to the prevention effect of cucumber fusarium axysporum and output.
Embodiment 17, the application of bio-feritlizer in control green pepper Powdery Mildew
Trial crops is divided into three groups: control group (CK does not apply fertilizer); Commercially available bio-feritlizer group (applying bio-feritlizer before field planting, 100kg/ mu); The bio-feritlizer group that the embodiment of the present invention 6 provides (applying bio-feritlizer before field planting, 100kg/ mu).Fertilization mode is all consistent with other farming methods, and in a conventional manner.
Every group of shared area of crop is 30m
2, every group arranges three repetitions, results averaged.The prevention effect of green pepper Powdery Mildew and the variation of output are as shown in table 8.
The prevention effect of table 8 green pepper Powdery Mildew and the variation of output
As can be seen from Table 8, use bio-feritlizer of the present invention to be all significantly improved than the commercially available bio-feritlizer of use to the prevention effect of green pepper Powdery Mildew and output.
It is example that above-described embodiment 11-17 be take the bio-feritlizer that the embodiment of the present invention provides, and the application to bio-feritlizer provided by the invention in improving soil physico-chemical property is described.
The raw material of using in above-described embodiment 1-18 is commercially available prod, comprises chemical fertilizer, fertilizer etc.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (10)
1. a microorganism, is characterized in that, preserving number is: CGMCC No.8435, preservation name is called: rhizopus stolonifer PF102 (Rhizopus stolonifer), preservation date is: on November 5th, 2013.
2. the application of microorganism claimed in claim 1 in bio-feritlizer preparation.
3. a bio-feritlizer, is characterized in that, comprises microorganism claimed in claim 1.
4. bio-feritlizer according to claim 3, is characterized in that, also comprises decomposed organic matter.
5. bio-feritlizer according to claim 4, is characterized in that, is made: the spore suspension of described microorganism: 5-15 part, decomposed organic matter: 30-50 part by the raw material of following weight part; Wherein, in described spore suspension, spore concentration alive is 3.0-5.0 * 10
10cfu/ml.
6. bio-feritlizer according to claim 5, is characterized in that, also comprises the chitin of 2-4 weight part.
7. bio-feritlizer according to claim 6, is characterized in that, also comprises the humic acid of 1-3 weight part.
8. bio-feritlizer according to claim 7, is characterized in that, also comprises the chitosan of 3-10 weight part.
9. bio-feritlizer according to claim 8, is characterized in that, is made: the spore suspension of described microorganism: 10 parts by the raw material of following weight part, described decomposed organic matter: 45 parts, described chitin: 3 parts, described humic acid: 2 parts, described chitosan: 10 parts.
10. the bio-feritlizer described in claim 3-9 any one is improving crop yield and the application in control crop pathogenic bacterium.
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| CN104130068A (en) * | 2014-08-06 | 2014-11-05 | 山东普方生物科技有限公司 | Compound multifunctional biological foliage fertilizer |
| CN104152149A (en) * | 2014-08-06 | 2014-11-19 | 山东普方生物科技有限公司 | Novel multifunctional composite biological soil improvement agent |
| CN104130068B (en) * | 2014-08-06 | 2016-08-17 | 山东普方生物科技有限公司 | A kind of composite multi-functional biological foliage fertilizer |
| CN105693385A (en) * | 2016-01-26 | 2016-06-22 | 陈琳 | Microbial fertilizer |
| CN109456900A (en) * | 2017-03-17 | 2019-03-12 | 山东普方生物科技有限公司 | A kind of composite biological agent and application thereof |
| CN109456900B (en) * | 2017-03-17 | 2022-09-20 | 山东普方生物科技有限公司 | Composite biological preparation and application thereof |
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